TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBS9h08.xg.2.1
(770 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF260956.1|BF260956 HVSMEf0023G04f Hordeum vulgare seedl... 105 9e-030
gb|BG309831.1|BG309831 HVSMEc0013A09f Hordeum vulgare seedl... 65 1e-027
gb|BE060120.2|BE060120 HVSMEg0011I12f Hordeum vulgare pre-a... 98 1e-027
gb|BF623091.2|BF623091 HVSMEa0014D14f Hordeum vulgare seedl... 85 3e-026
gb|BF631171.2|BF631171 HVSMEb0015C20f Hordeum vulgare seedl... 80 3e-022
gb|BG415849.1|BG415849 HVSMEk0008C14f Hordeum vulgare testa... 93 3e-022
>gb|BF260956.1|BF260956 HVSMEf0023G04f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0023G04f, mRNA sequence
Length = 746
Score = 105 bits (223), Expect(2) = 9e-030
Identities = 40/80 (50%), Positives = 53/80 (66%)
Frame = +1 / +1
Query: 334 SFVELFIRESEAVQSKAGRYQSLQEIPFFAYRVPSALAPPYNFTSDLYPAAXSVNVNDAI 513
S VE +RE+E + +K GRY+S E PFF +P L PP + + L+P A S+N+N I
Sbjct: 463 SLVEFMVRETEKLHAKVGRYKSPDEHPFFPEDLPETLLPPIQYPTVLHPIADSININKEI 642
Query: 514 WSMYFDELLPRLXKXGDDGN 573
W MYFDE++PRL K G DGN
Sbjct: 643 WKMYFDEVIPRLVKEGSDGN 702
Score = 45.1 bits (92), Expect(2) = 9e-030
Identities = 18/35 (51%), Positives = 25/35 (71%)
Frame = +1 / +1
Query: 193 LSLDTVRDFLTREEDTIVFSLIERAKYPLNRPAYD 297
L+LD++R L R ED+I+F L+ERA+Y N YD
Sbjct: 325 LTLDSIRTSLIRLEDSIIFGLLERAQYCYNADTYD 429
>gb|BG309831.1|BG309831 HVSMEc0013A09f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0013A09f, mRNA sequence
Length = 792
Score = 65.2 bits (136), Expect(3) = 1e-027
Identities = 30/68 (44%), Positives = 34/68 (50%)
Frame = +1 / +2
Query: 448 PPYNFTSDLYPAAXSVNVNDAIWSMYFDELLPRLXKXGDDGNYAVXXXXXXXXXXXXSXX 627
P + L+P A SV VNDAIW MYF+ELLP GDDGNYA S
Sbjct: 293 PTKGYPKVLHPFASSVCVNDAIWKMYFNELLPLFTADGDDGNYAETVALDFACLQALSRR 472
Query: 628 XNYXXYVA 651
+ YVA
Sbjct: 473 IHCGKYVA 496
Score = 55.6 bits (115), Expect(3) = 1e-027
Identities = 25/34 (73%), Positives = 27/34 (79%)
Frame = +1 / +1
Query: 193 LSLDTVRDFLTREEDTIVFSLIERAKYPLNRPAY 294
LSL VRD L REED+IVF+LIERAK P N PAY
Sbjct: 55 LSLAAVRDQLIREEDSIVFALIERAKRPRNAPAY 156
Score = 40.0 bits (81), Expect(3) = 1e-027
Identities = 13/34 (38%), Positives = 23/34 (67%)
Frame = +1 / +1
Query: 334 SFVELFIRESEAVQSKAGRYQSLQEIPFFAYRVP 435
S E F+RE++ + +KAG YQ +++PF + +P
Sbjct: 178 SVAEFFLREAQVLHAKAGHYQKPEDVPFLSQDLP 279
>gb|BE060120.2|BE060120 HVSMEg0011I12f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0011I12f, mRNA sequence
Length = 592
Score = 97.7 bits (207), Expect(2) = 1e-027
Identities = 38/80 (47%), Positives = 51/80 (63%)
Frame = +1 / +3
Query: 334 SFVELFIRESEAVQSKAGRYQSLQEIPFFAYRVPSALAPPYNFTSDLYPAAXSVNVNDAI 513
S VE +RE+E + +K GRY+S E PFF +P L PP + + L+P A S+N+N I
Sbjct: 147 SLVEFMVRETEKLHAKVGRYKSPDEHPFFPEDLPETLLPPIQYPTVLHPIADSININKEI 326
Query: 514 WSMYFDELLPRLXKXGDDGN 573
W MYFDE++ RL K DGN
Sbjct: 327 WKMYFDEVISRLVKERSDGN 386
Score = 45.1 bits (92), Expect(2) = 1e-027
Identities = 18/35 (51%), Positives = 25/35 (71%)
Frame = +1 / +3
Query: 193 LSLDTVRDFLTREEDTIVFSLIERAKYPLNRPAYD 297
L+LD++R L R ED+I+F L+ERA+Y N YD
Sbjct: 9 LTLDSIRTSLIRLEDSIIFGLLERAQYCYNADTYD 113
>gb|BF623091.2|BF623091 HVSMEa0014D14f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0014D14f, mRNA sequence
Length = 903
Score = 85.4 bits (180), Expect(2) = 3e-026
Identities = 32/82 (39%), Positives = 49/82 (59%)
Frame = +1 / +2
Query: 334 SFVELFIRESEAVQSKAGRYQSLQEIPFFAYRVPSALAPPYNFTSDLYPAAXSVNVNDAI 513
S E F+RE++ + +KAG YQ +++PF ++ +P + P + L+P+A S NDA
Sbjct: 152 SVAEFFVREAQVLHAKAGHYQKPEDVPFLSHDLPPPVFPTKGYPKVLHPSATSACENDAF 331
Query: 514 WSMYFDELLPRLXKXGDDGNYA 579
W MY +ELLP +GNYA
Sbjct: 332 WKMYLNELLPLFTADWHEGNYA 397
Score = 52.8 bits (109), Expect(2) = 3e-026
Identities = 24/34 (70%), Positives = 26/34 (76%)
Frame = +1 / +2
Query: 193 LSLDTVRDFLTREEDTIVFSLIERAKYPLNRPAY 294
LSL VRD L RE+D IVF+LIERAK P N PAY
Sbjct: 29 LSLAAVRDQLIREQDHIVFALIERAKRPRNAPAY 130
>gb|BF631171.2|BF631171 HVSMEb0015C20f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0015C20f, mRNA sequence
Length = 777
Score = 79.9 bits (168), Expect(2) = 3e-022
Identities = 30/65 (46%), Positives = 41/65 (63%)
Frame = +1 / +2
Query: 334 SFVELFIRESEAVQSKAGRYQSLQEIPFFAYRVPSALAPPYNFTSDLYPAAXSVNVNDAI 513
S VE +RE+E + +K GRY+S E PFF +P L PP + + L+P A S+N+N I
Sbjct: 482 SLVEFMVRETEKLHAKVGRYKSPDEHPFFPEDLPETLLPPIQYPTGLHPIADSININKEI 661
Query: 514 WSMYF 528
W MYF
Sbjct: 662 WKMYF 676
Score = 45.1 bits (92), Expect(2) = 3e-022
Identities = 18/35 (51%), Positives = 25/35 (71%)
Frame = +1 / +2
Query: 193 LSLDTVRDFLTREEDTIVFSLIERAKYPLNRPAYD 297
L+LD++R L R ED+I+F L+ERA+Y N YD
Sbjct: 344 LTLDSIRTSLIRLEDSIIFGLLERAQYCYNADTYD 448
>gb|BG415849.1|BG415849 HVSMEk0008C14f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0008C14f, mRNA sequence
Length = 660
Score = 93.2 bits (197), Expect(2) = 3e-022
Identities = 37/80 (46%), Positives = 50/80 (62%)
Frame = +1 / +1
Query: 334 SFVELFIRESEAVQSKAGRYQSLQEIPFFAYRVPSALAPPYNFTSDLYPAAXSVNVNDAI 513
S VE +RE E + + GRY+S E PFF +P L PP T+ L+P A S+N+N I
Sbjct: 166 SLVEYMVREPETLHPRVGRYKSPYEHPFFPEDLPDTLWPPIQHTAVLHPIADSLNINRQI 345
Query: 514 WSMYFDELLPRLXKXGDDGN 573
W M +DE++PRL K G +GN
Sbjct: 346 WKMSYDEVIPRLAKEGSEGN 405
Score = 31.8 bits (63), Expect(2) = 3e-022
Identities = 12/35 (34%), Positives = 21/35 (60%)
Frame = +1 / +1
Query: 193 LSLDTVRDFLTREEDTIVFSLIERAKYPLNRPAYD 297
L+LD++ R +D+ +F L+ERA++ YD
Sbjct: 28 LTLDSITTSWIRLQDSFIFGLLERAQFCYKADTYD 132
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 271,879,548
Number of Sequences: 312970
Number of extensions: 4202392
Number of successful extensions: 72271
Number of sequences better than 1.0e-020: 8
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 8589162756
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)