TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBJ4h04.xg.2.1
(393 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM442464.2|BM442464 EBan01_SQ003_E04_R anther, yellow st... 147 3e-035
gb|BQ753427.1|BQ753427 EBan01_SQ004_D21_R anther, yellow st... 147 3e-035
gb|BM442006.2|BM442006 EBan01_SQ001_K04_R anther, yellow st... 130 3e-030
gb|BQ753289.1|BQ753289 EBan01_SQ001_D10_R anther, yellow st... 130 3e-030
gb|BQ764354.1|BQ764354 EBan01_SQ005_I18_R anther, yellow st... 130 3e-030
gb|BM442216.2|BM442216 EBan01_SQ002_I23_R anther, yellow st... 108 2e-023
>gb|BM442464.2|BM442464 EBan01_SQ003_E04_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_E04 5', mRNA sequence
Length = 507
Score = 147 bits (315), Expect = 3e-035
Identities = 62/108 (57%), Positives = 64/108 (59%)
Frame = +3 / +1
Query: 69 MSFTGXXXKCTXCDKXXHFIDLLTXXXXIYHKTXXXXXHCKGXXSMXXYSSMDXXLYCKT 248
MSFTG KC+ C+K HFIDLLT IYHKT HCKG SM YSSMD LYCKT
Sbjct: 70 MSFTGTQDKCSACEKTVHFIDLLTADGVIYHKTCFKCSHCKGILSMCSYSSMDGVLYCKT 249
Query: 249 HFXQLFKXTGSFSKNFTPXXXXSXXXXLTRXPSKXXXXXSXXQDXXXA 392
HF QLFK TGSFSK FTP LTR PSK S QD A
Sbjct: 250 HFEQLFKETGSFSKKFTPGNKSGDKSELTRAPSKLSAAFSGTQDKCAA 393
Score = 107 bits (229), Expect = 2e-023
Identities = 56/109 (51%), Positives = 57/109 (52%)
Frame = -3 / -2
Query: 391 AXXLSWXXEXXXXXLLGXLVSXXLXDXXXXGVKFLEKLPVXLKSCXKWVLQYXXXSMEE* 212
A LS E LLG LVS L GV F EKLPV LKSC K VLQ SMEE*
Sbjct: 392 AAHLS*VPEKAADSLLGALVSSLLSPDLLPGVNFFEKLPVSLKSCSKCVLQ*RTPSMEE* 213
Query: 211 XXIEXXPLQWXXXXXVLW*XXXXXVRRSMKXXXLSXXVHLXXXPVKDMV 65
IE PLQW VLW* VRRSMK S HL PVKDM+
Sbjct: 212 LHIERIPLQWLHLKQVLW*MTPSAVRRSMKWTVFSHAEHLSCVPVKDML 66
Score = 100 bits (213), Expect = 4e-021
Identities = 50/94 (53%), Positives = 52/94 (55%)
Frame = +1 / +2
Query: 70 CLSPARXTSAXXATXXXTSSTSSXXXXXSTIRHXXXXXTAKGXSRCXATPPWTXXCTARP 249
CLSPAR TSA A TSSTSS ST R TAKG SRC A PWT TAR
Sbjct: 71 CLSPARRTSARRAKRRSTSSTSSRPTASSTTRPASNAATAKGSSRCAAILPWTVSSTARR 250
Query: 250 TSXSSSXXPGASPRTSRQXXXXQXXXN*QGXQAS 351
TS SSS GAS R+S + N*QG QAS
Sbjct: 251 TSSSSSRRQGASQRSSHRVTNLATRVN*QGPQAS 352
>gb|BQ753427.1|BQ753427 EBan01_SQ004_D21_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ004_D21 5', mRNA sequence
Length = 633
Score = 147 bits (315), Expect = 3e-035
Identities = 62/108 (57%), Positives = 64/108 (59%)
Frame = +3 / +2
Query: 69 MSFTGXXXKCTXCDKXXHFIDLLTXXXXIYHKTXXXXXHCKGXXSMXXYSSMDXXLYCKT 248
MSFTG KC+ C+K HFIDLLT IYHKT HCKG SM YSSMD LYCKT
Sbjct: 68 MSFTGTQDKCSACEKTVHFIDLLTADGVIYHKTCFKCSHCKGILSMCSYSSMDGVLYCKT 247
Query: 249 HFXQLFKXTGSFSKNFTPXXXXSXXXXLTRXPSKXXXXXSXXQDXXXA 392
HF QLFK TGSFSK FTP LTR PSK S QD A
Sbjct: 248 HFEQLFKETGSFSKKFTPGNKSGDKSELTRAPSKLSAAFSGTQDKCAA 391
Score = 107 bits (229), Expect = 2e-023
Identities = 56/109 (51%), Positives = 57/109 (52%)
Frame = -3 / -1
Query: 391 AXXLSWXXEXXXXXLLGXLVSXXLXDXXXXGVKFLEKLPVXLKSCXKWVLQYXXXSMEE* 212
A LS E LLG LVS L GV F EKLPV LKSC K VLQ SMEE*
Sbjct: 390 AAHLS*VPEKAADSLLGALVSSLLSPDLLPGVNFFEKLPVSLKSCSKCVLQ*RTPSMEE* 211
Query: 211 XXIEXXPLQWXXXXXVLW*XXXXXVRRSMKXXXLSXXVHLXXXPVKDMV 65
IE PLQW VLW* VRRSMK S HL PVKDM+
Sbjct: 210 LHIERIPLQWLHLKQVLW*MTPSAVRRSMKWTVFSHAEHLSCVPVKDML 64
Score = 100 bits (213), Expect = 4e-021
Identities = 50/94 (53%), Positives = 52/94 (55%)
Frame = +1 / +3
Query: 70 CLSPARXTSAXXATXXXTSSTSSXXXXXSTIRHXXXXXTAKGXSRCXATPPWTXXCTARP 249
CLSPAR TSA A TSSTSS ST R TAKG SRC A PWT TAR
Sbjct: 69 CLSPARRTSARRAKRRSTSSTSSRPTASSTTRPASNAATAKGSSRCAAILPWTVSSTARR 248
Query: 250 TSXSSSXXPGASPRTSRQXXXXQXXXN*QGXQAS 351
TS SSS GAS R+S + N*QG QAS
Sbjct: 249 TSSSSSRRQGASQRSSHRVTNLATRVN*QGPQAS 350
>gb|BM442006.2|BM442006 EBan01_SQ001_K04_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ001_K04 5', mRNA sequence
Length = 647
Score = 130 bits (279), Expect = 3e-030
Identities = 53/79 (67%), Positives = 53/79 (67%)
Frame = +3 / +3
Query: 66 TMSFTGXXXKCTXCDKXXHFIDLLTXXXXIYHKTXXXXXHCKGXXSMXXYSSMDXXLYCK 245
TMSFTG KC CDK HFIDLLT YHKT HCKG SM YSSMD LYCK
Sbjct: 108 TMSFTGTQDKCKTCDKTVHFIDLLTADGVSYHKTCFKCSHCKGTLSMCNYSSMDGVLYCK 287
Query: 246 THFXQLFKXTGSFSKNFTP 302
THF QLFK TGSFSK FTP
Sbjct: 288 THFEQLFKETGSFSKKFTP 344
Score = 104 bits (221), Expect = 3e-022
Identities = 48/79 (60%), Positives = 49/79 (62%)
Frame = -3 / -2
Query: 301 GVKFLEKLPVXLKSCXKWVLQYXXXSMEE*XXIEXXPLQWXXXXXVLW*XXXXXVRRSMK 122
GV F EKLPV LKSC KWVLQ SMEE* IE PLQW VLW* VRRSMK
Sbjct: 343 GVNFFEKLPVSLKSCSKWVLQ*RTPSMEE*LHIERVPLQWLHLKHVLW*ETPSAVRRSMK 164
Query: 121 XXXLSXXVHLXXXPVKDMV 65
LS +HL PVKDMV
Sbjct: 163 WTVLSHVLHLSCVPVKDMV 107
>gb|BQ753289.1|BQ753289 EBan01_SQ001_D10_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ001_D10 5', mRNA sequence
Length = 634
Score = 130 bits (279), Expect = 3e-030
Identities = 53/79 (67%), Positives = 53/79 (67%)
Frame = +3 / +3
Query: 66 TMSFTGXXXKCTXCDKXXHFIDLLTXXXXIYHKTXXXXXHCKGXXSMXXYSSMDXXLYCK 245
TMSFTG KC CDK HFIDLLT YHKT HCKG SM YSSMD LYCK
Sbjct: 114 TMSFTGTQDKCKTCDKTVHFIDLLTADGVSYHKTCFKCSHCKGTLSMCNYSSMDGVLYCK 293
Query: 246 THFXQLFKXTGSFSKNFTP 302
THF QLFK TGSFSK FTP
Sbjct: 294 THFEQLFKETGSFSKKFTP 350
Score = 104 bits (221), Expect = 3e-022
Identities = 48/79 (60%), Positives = 49/79 (62%)
Frame = -3 / -1
Query: 301 GVKFLEKLPVXLKSCXKWVLQYXXXSMEE*XXIEXXPLQWXXXXXVLW*XXXXXVRRSMK 122
GV F EKLPV LKSC KWVLQ SMEE* IE PLQW VLW* VRRSMK
Sbjct: 349 GVNFFEKLPVSLKSCSKWVLQ*RTPSMEE*LHIERVPLQWLHLKHVLW*ETPSAVRRSMK 170
Query: 121 XXXLSXXVHLXXXPVKDMV 65
LS +HL PVKDMV
Sbjct: 169 WTVLSHVLHLSCVPVKDMV 113
>gb|BQ764354.1|BQ764354 EBan01_SQ005_I18_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ005_I18 5', mRNA sequence
Length = 598
Score = 130 bits (279), Expect = 3e-030
Identities = 53/79 (67%), Positives = 53/79 (67%)
Frame = +3 / +3
Query: 66 TMSFTGXXXKCTXCDKXXHFIDLLTXXXXIYHKTXXXXXHCKGXXSMXXYSSMDXXLYCK 245
TMSFTG KC CDK HFIDLLT YHKT HCKG SM YSSMD LYCK
Sbjct: 117 TMSFTGTQDKCKTCDKTVHFIDLLTADGVSYHKTCFKCSHCKGTLSMCNYSSMDGVLYCK 296
Query: 246 THFXQLFKXTGSFSKNFTP 302
THF QLFK TGSFSK FTP
Sbjct: 297 THFEQLFKETGSFSKKFTP 353
Score = 104 bits (221), Expect = 3e-022
Identities = 48/79 (60%), Positives = 49/79 (62%)
Frame = -3 / -1
Query: 301 GVKFLEKLPVXLKSCXKWVLQYXXXSMEE*XXIEXXPLQWXXXXXVLW*XXXXXVRRSMK 122
GV F EKLPV LKSC KWVLQ SMEE* IE PLQW VLW* VRRSMK
Sbjct: 352 GVNFFEKLPVSLKSCSKWVLQ*RTPSMEE*LHIERVPLQWLHLKHVLW*ETPSAVRRSMK 173
Query: 121 XXXLSXXVHLXXXPVKDMV 65
LS +HL PVKDMV
Sbjct: 172 WTVLSHVLHLSCVPVKDMV 116
>gb|BM442216.2|BM442216 EBan01_SQ002_I23_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ002_I23 5', mRNA sequence
Length = 284
Score = 108 bits (230), Expect = 2e-023
Identities = 43/76 (56%), Positives = 47/76 (61%)
Frame = +3 / +1
Query: 69 MSFTGXXXKCTXCDKXXHFIDLLTXXXXIYHKTXXXXXHCKGXXSMXXYSSMDXXLYCKT 248
M+F G KC C+K HFIDLLT YHK HCK SM YSSMD L+CKT
Sbjct: 49 MTFYGTQDKCKACNKTVHFIDLLTADSVPYHKYCFKCSHCKSTLSMCSYSSMDGVLFCKT 228
Query: 249 HFXQLFKXTGSFSKNF 296
HF QLFK TG+F KNF
Sbjct: 229 HFEQLFKETGTFKKNF 276
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 114,366,990
Number of Sequences: 312970
Number of extensions: 1169845
Number of successful extensions: 90405
Number of sequences better than 1.0e-020: 6
length of database: 58,378,179
effective HSP length: 49
effective length of database: 43,042,649
effective search space used: 3486454569
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)