TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBJ23f06.xg.2.1
(683 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ465022.1|BQ465022 HU02G23r HU Hordeum vulgare subsp. v... 274 4e-073
gb|BI948612.1|BI948612 HVSMEl0010E03f Hordeum vulgare spike... 236 9e-062
gb|BF258077.2|BF258077 HVSMEf0014L07f Hordeum vulgare seedl... 186 1e-046
gb|BF262816.1|BF262816 HV_CEa0005A16f Hordeum vulgare seedl... 110 5e-037
gb|AL506006.1|AL506006 AL506006 Hordeum vulgare Barke devel... 91 4e-033
gb|CA025160.1|CA025160 HZ51G03r HZ Hordeum vulgare subsp. v... 91 8e-033
gb|CA020774.1|CA020774 HZ37K18r HZ Hordeum vulgare subsp. v... 91 2e-032
gb|BF256073.3|BF256073 HVSMEf0008K14f Hordeum vulgare seedl... 137 8e-032
gb|BF254331.2|BF254331 HVSMEf0003K19f Hordeum vulgare seedl... 135 2e-031
gb|AL450690.1|AL450690 AL450690 Hordeum vulgare Barke etiol... 91 3e-031
gb|BI957573.1|BI957573 HVSMEn0010D05f Hordeum vulgare rachi... 91 3e-031
gb|BI958463.1|BI958463 HVSMEn0015C15f Hordeum vulgare rachi... 74 5e-028
gb|BU994896.1|BU994896 HM08I11r HM Hordeum vulgare subsp. v... 91 3e-027
gb|BU989284.1|BU989284 HF21F01r HF Hordeum vulgare subsp. v... 118 3e-026
gb|CA022440.1|CA022440 HZ43D05r HZ Hordeum vulgare subsp. v... 91 3e-026
gb|AV926474.1|AV926474 AV926474 K. Sato unpublished cDNA li... 91 3e-026
gb|AL507314.1|AL507314 AL507314 Hordeum vulgare Barke devel... 91 4e-026
gb|BF629785.2|BF629785 HVSMEb0013M23f Hordeum vulgare seedl... 93 2e-025
gb|BG309045.2|BG309045 HVSMEc0001K22f Hordeum vulgare seedl... 68 5e-025
gb|BQ459986.1|BQ459986 HA07I14r HA Hordeum vulgare subsp. v... 95 2e-024
>gb|BQ465022.1|BQ465022 HU02G23r HU Hordeum vulgare subsp. vulgare cDNA clone HU02G23
5-PRIME, mRNA sequence
Length = 700
Score = 274 bits (592), Expect = 4e-073
Identities = 114/135 (84%), Positives = 123/135 (91%)
Frame = +1 / +2
Query: 40 AMASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHE 219
AMASLTLPPA NPRQDAIDLQKAFKGFGCDSTTV NIL HRDS+QRG IQQEY+ MY E
Sbjct: 296 AMASLTLPPAPPNPRQDAIDLQKAFKGFGCDSTTVSNILAHRDSMQRGYIQQEYKTMYSE 475
Query: 220 ELSHRISSELNGNHKKAMLLWILDPAGRDXTVLREALSGDTMDLRAATDIICSRTPSQLX 399
ELS RISSEL+GNHKKAM LWILDPAGRD TVL+EALS +++DL+AATDIICSRTPSQL
Sbjct: 476 ELSRRISSELSGNHKKAMSLWILDPAGRDATVLKEALSAESLDLKAATDIICSRTPSQLQ 655
Query: 400 IMKQTYYARFGTYLE 444
IMKQTYYA+FGTYLE
Sbjct: 656 IMKQTYYAKFGTYLE 700
Score = 238 bits (514), Expect = 3e-062
Identities = 108/135 (80%), Positives = 116/135 (85%)
Frame = -1 / -2
Query: 443 SR*VPNLA**VCFIIXNCDGVLEHIISVAALRSIVSPLRASLKTVXSRPAGSRIHSNIAF 264
SR*VPN A* VCFII +CDGVLEHIISVAA RS +S LRAS +TV SRPAGSRI S+IAF
Sbjct: 699 SR*VPNFA*YVCFIICSCDGVLEHIISVAAFRSRLSALRASFRTVASRPAGSRIQSDIAF 520
Query: 263 LWFPLSSDEMRWESSS*YIALYSC*MSPRCTESR*VSMFITVVLSQPNPLKAFWRSMASC 84
LWFPLSSD++R ESSS YI LYSC*M PRC ESR* S+F TVVLSQPNPLKAFWRSMAS
Sbjct: 519 LWFPLSSDDIRRESSSEYIVLYSC*MYPRCIESR*ASIFETVVLSQPNPLKAFWRSMASW 340
Query: 83 RGFAIAGGRVRLAIA 39
RGF AGG+VRLAIA
Sbjct: 339 RGFGGAGGKVRLAIA 295
Score = 171 bits (369), Expect = 3e-042
Identities = 81/117 (69%), Positives = 86/117 (73%)
Frame = -2 / -3
Query: 442 QDKYQI*HNKSVSLFXTVMVSWNI*YQWLLSDP*CHHLELLSKQXXHVQQDQESTAT*LS 263
QDKYQ HN SVSLF VMVS NI YQ LLSD H ELLS+Q HVQQDQES AT LS
Sbjct: 698 QDKYQTLHNMSVSLFAAVMVSSNILYQ*LLSDQDFQH*ELLSEQSHHVQQDQESRATLLS 519
Query: 262 CGFH*VQMKCDGRVPHDT*PCIPVE*VHAALNRDE*VCLSLLYCHSQTL*RPFGGRW 92
CGFH*VQ D +VP +T* CIPVE +H AL+ E V L LLY HSQTL*RPFGGRW
Sbjct: 518 CGFH*VQTIYDAKVPRNT*FCIPVECIHVALSHGEQVYLKLLYYHSQTL*RPFGGRW 348
Score = 171 bits (368), Expect = 3e-042
Identities = 83/122 (68%), Positives = 92/122 (75%)
Frame = +2 / +3
Query: 77 THGRTPSTSKRPSKGLAVTVQQ**TYLLIAIQCSVDSFNRNTGLCIMRNSPIAFHLNSME 256
T RTPSTSKRPSKGLAV VQQ YLL QC+VD+FNRNT LCI RN + + LNS+E
Sbjct: 333 TPARTPSTSKRPSKGLAVIVQQFQIYLLTVTQCNVDTFNRNTKLCIPRNFRVVYRLNSVE 512
Query: 257 TTRKLCCCGFLILLDVXXLF*EKL*VVTLWI*EQPLILYVPGHHHSXK**NRLIMLDLVL 436
TTRK C GFLILLDV LF*+KL V+ + I*+Q LI YV GHHHS K**NR IM LVL
Sbjct: 513 TTRKQCRSGFLILLDVMRLF*KKLSVLKVLI*KQLLI*YVRGHHHSCK**NRHIMQSLVL 692
Query: 437 IL 442
IL
Sbjct: 693 IL 698
Score = 159 bits (342), Expect(2) = 2e-047
Identities = 68/98 (69%), Positives = 75/98 (76%)
Frame = +3 / +1
Query: 39 GDGEPHPAAGDGEPTAGRHRPPKGLQRVWL*QYNSDKHTYSSRFSAAWTHSTGIQGYVS* 218
G+GEPH AAG EP GRHRPPKGLQRVWL* YNS K+T S +A W HSTGIQ YV
Sbjct: 295 GNGEPHLAAGAPEPPPGRHRPPKGLQRVWL**YNSFKYTCSP*LNATWIHSTGIQNYVFR 474
Query: 219 GTLPSHFI*TQWKPQESYVAVDS*SCWT*XXCFERSSK 332
GT S+ +*TQWKPQES VA+DS*SCWT* C ERSS+
Sbjct: 475 GTFASYIV*TQWKPQESNVALDS*SCWT*CDCSERSSQ 588
Score = 156 bits (336), Expect = 9e-038
Identities = 69/112 (61%), Positives = 78/112 (69%)
Frame = -3 / -1
Query: 411 LFHYXQL*WCPGTYNISGCSQIHSVXXXXXXXXXXXXXSRIKNPQQHSFLVVSIEFR*NA 232
LFHY QL*WCP TY IS C QI + SRIKNP++H FLVVS EFR
Sbjct: 667 LFHYLQL*WCPRTYYISSCFQIKTFSTESFFQNSRITSSRIKNPERHCFLVVSTEFRRYT 488
Query: 231 MGEFLMIHSPVFLLNESTLH*IAMSKYVYHCCTVTAKPFEGLLEVDGVLPWV 76
+FL IHS VFLLN STLH*+ +SKY+ +CCT+TAKPFEGLLEVDGVL V
Sbjct: 487 TRKFLGIHSFVFLLNVSTLH*VTVSKYI*NCCTITAKPFEGLLEVDGVLAGV 332
Score = 49.6 bits (102), Expect(2) = 2e-047
Identities = 18/24 (75%), Positives = 21/24 (87%)
Frame = +3 / +1
Query: 372 MFQDTITVXNNETDLLC*IWYLS* 443
MF+DTIT NNETD+LC +WYLS*
Sbjct: 628 MFEDTITAANNETDILCKVWYLS* 699
>gb|BI948612.1|BI948612 HVSMEl0010E03f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0010E03f, mRNA sequence
Length = 812
Score = 236 bits (510), Expect = 9e-062
Identities = 92/146 (63%), Positives = 119/146 (81%)
Frame = +1 / +2
Query: 34 LPAMASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMY 213
L +MASL++PP + +PR DAI L +AFKGFGCDSTTVINIL HRD+ QR LI QEYRA+Y
Sbjct: 41 LRSMASLSVPPVLTSPRHDAIALHRAFKGFGCDSTTVINILAHRDATQRALIMQEYRAIY 220
Query: 214 HEELSHRISSELNGNHKKAMLLWILDPAGRDXTVLREALSGDTMDLRAATDIICSRTPSQ 393
H++L HR+S+EL+GNHKKAMLLW+LDP GRD +L ++L+GD DLRAAT++ICS+TP Q
Sbjct: 221 HQDLYHRLSTELSGNHKKAMLLWVLDPVGRDAAILNQSLNGDITDLRAATEVICSQTP*Q 400
Query: 394 LXIMKQTYYARFGTYLEHDIGHHTSG 471
L +M+Q Y ARFG YL+ + + +G
Sbjct: 401 LHMMRQGYRARFGCYLKRTVPYALTG 478
Score = 167 bits (359), Expect = 6e-041
Identities = 81/137 (59%), Positives = 91/137 (66%)
Frame = -1 / -3
Query: 440 R*VPNLA**VCFIIXNCDGVLEHIISVAALRSIVSPLRASLKTVXSRPAGSRIHSNIAFL 261
R* P A * C I+ +C GV E + SVAA RS++SPLR L+ SRP GS HS+IAFL
Sbjct: 447 R*HPKRAR*PCLIMCSC*GVWEQMTSVAARRSVMSPLRDWLRMAASRPTGSSTHSSIAFL 268
Query: 260 WFPLSSDEMRWESSS*YIALYSC*MSPRCTESR*VSMFITVVLSQPNPLKAFWRSMASCR 81
WFPLSS E RW S +ALYSC MS RC SR M ITVVLSQP PLKA WR+MAS R
Sbjct: 267 WFPLSSVERRWYRSWW*MALYSCMMSARCVASRCARMLITVVLSQPKPLKALWRAMASWR 88
Query: 80 GFAIAGGRVRLAIAGNR 30
G GG +RLAI R
Sbjct: 87 GDVSTGGTLRLAIDRRR 37
>gb|BF258077.2|BF258077 HVSMEf0014L07f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0014L07f, mRNA sequence
Length = 573
Score = 186 bits (401), Expect = 1e-046
Identities = 74/112 (66%), Positives = 86/112 (76%)
Frame = +1 / +3
Query: 346 DLRAATDIICSRTPSQLXIMKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYVGIPRYEGP 525
DLRAAT++ICSRTPSQL IMKQTY ARFG YLEHDI T GDHQ LLLAY+G+PR EGP
Sbjct: 3 DLRAATEVICSRTPSQLQIMKQTYRARFGCYLEHDITERTYGDHQKLLLAYLGVPRNEGP 182
Query: 526 EVDPTIXTHDAKDLYKAGEXRLGTDEXXXXXXFTXRXWAHXASXSSAXHHMY 681
EVDP++ T DA++LY+ GE R+GTDE F+ R WAH AS + A HMY
Sbjct: 183 EVDPSVVTDDARELYRTGEKRVGTDERAFIRIFSERSWAHLASVAKAYQHMY 338
Score = 112 bits (240), Expect = 2e-024
Identities = 62/113 (54%), Positives = 67/113 (59%)
Frame = -1 / -3
Query: 683 SYI*WXADXXDAXCAQXRXVXXXXXXXXXXXXXXXPALYKSFASCVXMVGSTSGPS*RGI 504
+Y+ W A DA CAQ R + P Y S AS V GSTSGPS RG
Sbjct: 340 AYMCW*ALATDARCAQLRSLKMRMKARSSVPTLFSPVRYSSLASSVTTEGSTSGPSFRGT 161
Query: 503 PT*ASKSXW*SPDVW*PMSCSR*VPNLA**VCFIIXNCDGVLEHIISVAALRS 345
P ASKS *SP V MSCSR* PNLA *VCF+I +CDGVLE I SVAALRS
Sbjct: 160 PRYASKSF**SP*VRSVMSCSR*QPNLAR*VCFMICSCDGVLEQITSVAALRS 2
>gb|BF262816.1|BF262816 HV_CEa0005A16f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0005A16f, mRNA sequence
Length = 868
Score = 110 bits (235), Expect(2) = 5e-037
Identities = 46/65 (70%), Positives = 51/65 (78%)
Frame = +1 / +1
Query: 286 LDPAGRDXTVLREALSGDTMDLRAATDIICSRTPSQLXIMKQTYYARFGTYLEHDIGHHT 465
L AGRD T+L +AL+GD DLRAAT++ICSRTPSQL IMKQTY ARFG YLE DI T
Sbjct: 19 LKHAGRDATILNQALNGDITDLRAATEVICSRTPSQLQIMKQTYRARFGCYLERDITERT 198
Query: 466 SGDHQ 480
GDHQ
Sbjct: 199 YGDHQ 213
Score = 66.1 bits (138), Expect(2) = 5e-037
Identities = 26/40 (65%), Positives = 33/40 (82%)
Frame = +1 / +2
Query: 484 LLLAYVGIPRYEGPEVDPTIXTHDAKDLYKAGEXRLGTDE 603
LLLAY+G+PR EGPEVDP+ T DA++L + GE R+GTDE
Sbjct: 338 LLLAYLGVPRNEGPEVDPSGVTDDARELDRTGEKRVGTDE 457
>gb|AL506006.1|AL506006 AL506006 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY01G09T 5',
mRNA sequence
Length = 700
Score = 91.3 bits (193), Expect(2) = 4e-033
Identities = 38/94 (40%), Positives = 60/94 (63%)
Frame = +1 / +1
Query: 43 MASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEE 222
MA+L +P + D +L+KAF+G+G + +I+IL HRD+ QR I++ Y Y EE
Sbjct: 106 MATLKVPSNVPALADDCDNLRKAFQGWGTNEALIISILGHRDAAQRRAIRKHYADTYGEE 285
Query: 223 LSHRISSELNGNHKKAMLLWILDPAGRDXTVLRE 324
L I+ E++G+ ++A++LW LDPA RD + E
Sbjct: 286 LLRSITDEISGDFERAVILWTLDPAERDAVLANE 387
Score = 69.8 bits (146), Expect(2) = 4e-033
Identities = 31/80 (38%), Positives = 47/80 (58%)
Frame = +1 / +1
Query: 364 DIICSRTPSQLXIMKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYVGIPRYEGPEVDPTI 543
+I CSR +QL ++Q Y+ RF LE D+ H +GD + LL+ V RYEGPEV+ +
Sbjct: 427 EIACSRGSAQLFAVRQAYHDRFKRSLEEDVAAHVTGDFRKLLVPLVSSYRYEGPEVNTRL 606
Query: 544 XTHDAKDLYKAGEXRLGTDE 603
+AK L++ E + D+
Sbjct: 607 AHSEAKILHEKIEHKAYGDD 666
>gb|CA025160.1|CA025160 HZ51G03r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ51G03
5-PRIME, mRNA sequence
Length = 636
Score = 91.3 bits (193), Expect(2) = 8e-033
Identities = 38/94 (40%), Positives = 60/94 (63%)
Frame = +1 / +3
Query: 43 MASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEE 222
MA+L +P + D +L+KAF+G+G + +I+IL HRD+ QR I++ Y Y EE
Sbjct: 87 MATLKVPSNVPALADDCDNLRKAFQGWGTNEALIISILGHRDAAQRRAIRKHYADTYGEE 266
Query: 223 LSHRISSELNGNHKKAMLLWILDPAGRDXTVLRE 324
L I+ E++G+ ++A++LW LDPA RD + E
Sbjct: 267 LLRSITDEISGDFERAVILWTLDPAERDAVLANE 368
Score = 68.9 bits (144), Expect(2) = 8e-033
Identities = 30/75 (40%), Positives = 45/75 (60%)
Frame = +1 / +3
Query: 364 DIICSRTPSQLXIMKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYVGIPRYEGPEVDPTI 543
+I CSR +QL ++Q Y+ RF LE D+ H +GD + LL+ V RYEGPEV+ +
Sbjct: 408 EIACSRGSAQLFAVRQAYHDRFKRSLEEDVAAHVTGDFRKLLVPLVSSYRYEGPEVNTRL 587
Query: 544 XTHDAKDLYKAGEXR 588
+AK L++ E +
Sbjct: 588 AHSEAKILHEKIEHK 632
>gb|CA020774.1|CA020774 HZ37K18r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ37K18
5-PRIME, mRNA sequence
Length = 618
Score = 91.3 bits (193), Expect(2) = 2e-032
Identities = 38/94 (40%), Positives = 60/94 (63%)
Frame = +1 / +1
Query: 43 MASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEE 222
MA+L +P + D +L+KAF+G+G + +I+IL HRD+ QR I++ Y Y EE
Sbjct: 91 MATLKVPSNVPALADDCDNLRKAFQGWGTNEALIISILGHRDAAQRRAIRKHYADTYGEE 270
Query: 223 LSHRISSELNGNHKKAMLLWILDPAGRDXTVLRE 324
L I+ E++G+ ++A++LW LDPA RD + E
Sbjct: 271 LLRSITDEISGDFERAVILWTLDPAERDAVLANE 372
Score = 68.0 bits (142), Expect(2) = 2e-032
Identities = 29/69 (42%), Positives = 42/69 (60%)
Frame = +1 / +1
Query: 364 DIICSRTPSQLXIMKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYVGIPRYEGPEVDPTI 543
+I CSR +QL ++Q Y+ RF LE D+ H +GD + LL+ V RYEGPEV+ +
Sbjct: 412 EIACSRGSAQLFAVRQAYHDRFKRSLEEDVAAHVTGDFRKLLVPLVSSYRYEGPEVNTRL 591
Query: 544 XTHDAKDLY 570
+AK L+
Sbjct: 592 AHSEAKILH 618
>gb|BF256073.3|BF256073 HVSMEf0008K14f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0008K14f, mRNA sequence
Length = 836
Score = 137 bits (293), Expect = 8e-032
Identities = 56/92 (60%), Positives = 65/92 (70%)
Frame = +1 / +2
Query: 370 ICSRTPSQLXIMKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYVGIPRYEGPEVDPTIXT 549
ICS TPSQL IMKQTY ARFG YLEHDI T GDHQ LLLA +G+PR EGPEVDP+ T
Sbjct: 2 ICSTTPSQLQIMKQTYRARFGCYLEHDITERTYGDHQKLLLACLGVPRNEGPEVDPSEVT 181
Query: 550 HDAKDLYKAGEXRLGTDEXXXXXXFTXRXWAH 645
DA++L + G+ R+GTDE + R W H
Sbjct: 182 DDARELCRTGKKRVGTDERAFVRVLSERDWTH 277
>gb|BF254331.2|BF254331 HVSMEf0003K19f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0003K19f, mRNA sequence
Length = 524
Score = 135 bits (290), Expect = 2e-031
Identities = 60/160 (37%), Positives = 91/160 (56%)
Frame = +1 / +3
Query: 22 PAQRLPAMASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEY 201
P + P MA+L++P A+ +D L+KAF G+G + +I+IL HRD+ QR I++ Y
Sbjct: 39 PDRTGPEMATLSVPAAVPAVAEDCEQLRKAFAGWGTNERLIISILAHRDAAQRRAIRKHY 218
Query: 202 RAMYHEELSHRISSELNGNHKKAMLLWILDPAGRDXTVLREALSGDTMDLRAATDIICSR 381
Y EEL I+ E++G ++A++ W LDPA RD + E RA +I C+R
Sbjct: 219 ADAYGEELLRSITDEIHGKFERAVIQWTLDPAERDAVLANEEAKKWNPGGRALVEIACAR 398
Query: 382 TPSQLXIMKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYV 501
TP+ L KQ Y+ R LE D+ H +GD + L++ V
Sbjct: 399 TPAHLFAAKQAYHDRLKRSLEEDVAAHVTGDFRKLVVPLV 518
>gb|AL450690.1|AL450690 AL450690 Hordeum vulgare Barke etiolated leaves Hordeum vulgare
subsp. vulgare cDNA clone HK03M19u 5', mRNA sequence
Length = 657
Score = 91.3 bits (193), Expect(2) = 3e-031
Identities = 38/94 (40%), Positives = 60/94 (63%)
Frame = +1 / +2
Query: 43 MASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEE 222
MA+L +P + D +L+KAF+G+G + +I+IL HRD+ QR I++ Y Y EE
Sbjct: 89 MATLKVPSNVPALADDCDNLRKAFQGWGTNEALIISILGHRDAAQRRAIRKHYADTYGEE 268
Query: 223 LSHRISSELNGNHKKAMLLWILDPAGRDXTVLRE 324
L I+ E++G+ ++A++LW LDPA RD + E
Sbjct: 269 LLRSITDEISGDFERAVILWTLDPAERDAVLANE 370
Score = 63.8 bits (133), Expect(2) = 3e-031
Identities = 27/62 (43%), Positives = 37/62 (59%)
Frame = +1 / +2
Query: 364 DIICSRTPSQLXIMKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYVGIPRYEGPEVDPTI 543
+I CSR +QL ++Q Y+ RF LE D+ H +GD + LL+ V RYEGPEV+
Sbjct: 410 EIACSRGSAQLFAVRQAYHDRFKRSLEEDVAAHVTGDFRKLLVPLVSSYRYEGPEVNTRF 589
Query: 544 XT 549
T
Sbjct: 590 GT 595
>gb|BI957573.1|BI957573 HVSMEn0010D05f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0010D05f, mRNA sequence
Length = 628
Score = 91.3 bits (193), Expect(2) = 3e-031
Identities = 38/94 (40%), Positives = 60/94 (63%)
Frame = +1 / +1
Query: 43 MASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEE 222
MA+L +P + D +L+KAF+G+G + +I+IL HRD+ QR I++ Y Y EE
Sbjct: 58 MATLKVPSNVPALADDCDNLRKAFQGWGTNEALIISILGHRDAAQRRAIRKHYADTYGEE 237
Query: 223 LSHRISSELNGNHKKAMLLWILDPAGRDXTVLRE 324
L I+ E++G+ ++A++LW LDPA RD + E
Sbjct: 238 LLRSITDEISGDFERAVILWTLDPAERDAVLANE 339
Score = 63.8 bits (133), Expect(2) = 3e-031
Identities = 27/62 (43%), Positives = 37/62 (59%)
Frame = +1 / +1
Query: 364 DIICSRTPSQLXIMKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYVGIPRYEGPEVDPTI 543
+I CSR +QL ++Q Y+ RF LE D+ H +GD + LL+ V RYEGPEV+
Sbjct: 379 EIACSRGSAQLFAVRQAYHDRFKRSLEEDVAAHVTGDFRKLLVPLVSSYRYEGPEVNTRF 558
Query: 544 XT 549
T
Sbjct: 559 GT 564
>gb|BI958463.1|BI958463 HVSMEn0015C15f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0015C15f, mRNA sequence
Length = 656
Score = 74.4 bits (156), Expect(2) = 5e-028
Identities = 29/69 (42%), Positives = 45/69 (65%)
Frame = +1 / +1
Query: 118 GFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEELSHRISSELNGNHKKAMLLWILDPA 297
G+G + +I+IL HRD+ QR I++ Y Y EEL I+ E++G+ ++A++LW LDPA
Sbjct: 34 GWGTNEALIISILGHRDAAQRRAIRKHYADTYGEELLRSITDEISGDFERAVILWTLDPA 213
Query: 298 GRDXTVLRE 324
RD + E
Sbjct: 214 ERDAVLANE 240
Score = 69.8 bits (146), Expect(2) = 5e-028
Identities = 31/80 (38%), Positives = 47/80 (58%)
Frame = +1 / +1
Query: 364 DIICSRTPSQLXIMKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYVGIPRYEGPEVDPTI 543
+I CSR +QL ++Q Y+ RF LE D+ H +GD + LL+ V RYEGPEV+ +
Sbjct: 280 EIACSRGSAQLFAVRQAYHDRFKRSLEEDVAAHVTGDFRKLLVPLVSSYRYEGPEVNTRL 459
Query: 544 XTHDAKDLYKAGEXRLGTDE 603
+AK L++ E + D+
Sbjct: 460 AHSEAKILHEKIEHKAYGDD 519
>gb|BU994896.1|BU994896 HM08I11r HM Hordeum vulgare subsp. vulgare cDNA clone HM08I11
5-PRIME, mRNA sequence
Length = 568
Score = 91.3 bits (193), Expect(2) = 3e-027
Identities = 38/94 (40%), Positives = 60/94 (63%)
Frame = +1 / +1
Query: 43 MASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEE 222
MA+L +P + D +L+KAF+G+G + +I+IL HRD+ QR I++ Y Y EE
Sbjct: 94 MATLKVPSNVPALADDCDNLRKAFQGWGTNEALIISILGHRDAAQRRAIRKHYADTYGEE 273
Query: 223 LSHRISSELNGNHKKAMLLWILDPAGRDXTVLRE 324
L I+ E++G+ ++A++LW LDPA RD + E
Sbjct: 274 LLRSITDEISGDFERAVILWTLDPAERDAVLANE 375
Score = 50.1 bits (103), Expect(2) = 3e-027
Identities = 21/51 (41%), Positives = 30/51 (58%)
Frame = +1 / +1
Query: 364 DIICSRTPSQLXIMKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYVGIPRY 516
+I CSR +QL ++Q Y+ RF LE D+ H +GD + LL+ V RY
Sbjct: 415 EIACSRGSAQLFAVRQAYHDRFKRSLEEDVAAHVTGDFRKLLVPLVSSYRY 567
>gb|BU989284.1|BU989284 HF21F01r HF Hordeum vulgare subsp. vulgare cDNA clone HF21F01
5-PRIME, mRNA sequence
Length = 536
Score = 118 bits (253), Expect = 3e-026
Identities = 52/135 (38%), Positives = 77/135 (57%)
Frame = +1 / +3
Query: 166 DSVQRGLIQQEYRAMYHEELSHRISSELNGNHKKAMLLWILDPAGRDXTVLREALSGDTM 345
D+ QR I++ Y Y EEL I+ E++G ++A++ W LDPA RD + E
Sbjct: 3 DAAQRRAIRKHYADAYGEELLRSITDEIHGKFERAVIQWTLDPAERDAVLANEEAKKWNP 182
Query: 346 DLRAATDIICSRTPSQLXIMKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYVGIPRYEGP 525
RA +I C+RTP+ L KQ Y+ RF LE D+ H +GD + LL+ V RY+GP
Sbjct: 183 GGRALVEIACARTPAHLFAAKQAYHDRFKRSLEEDVAAHVTGDFRKLLVPLVSAYRYDGP 362
Query: 526 EVDPTIXTHDAKDLY 570
EV+ ++ +AK L+
Sbjct: 363 EVNTSLAHSEAKILH 407
>gb|CA022440.1|CA022440 HZ43D05r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ43D05
5-PRIME, mRNA sequence
Length = 575
Score = 91.3 bits (193), Expect(2) = 3e-026
Identities = 38/94 (40%), Positives = 60/94 (63%)
Frame = +1 / +2
Query: 43 MASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEE 222
MA+L +P + D +L+KAF+G+G + +I+IL HRD+ QR I++ Y Y EE
Sbjct: 110 MATLKVPSNVPALADDCDNLRKAFQGWGTNEALIISILGHRDAAQRRAIRKHYADTYGEE 289
Query: 223 LSHRISSELNGNHKKAMLLWILDPAGRDXTVLRE 324
L I+ E++G+ ++A++LW LDPA RD + E
Sbjct: 290 LLRSITDEISGDFERAVILWTLDPAERDAVLANE 391
Score = 46.9 bits (96), Expect(2) = 3e-026
Identities = 19/46 (41%), Positives = 28/46 (60%)
Frame = +1 / +2
Query: 364 DIICSRTPSQLXIMKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYV 501
+I CSR +QL ++Q Y+ RF LE D+ H +GD + LL+ V
Sbjct: 431 EIACSRGSAQLFAVRQAYHDRFKRSLEEDVAAHVTGDFRKLLVPLV 568
>gb|AV926474.1|AV926474 AV926474 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd19i03 5', mRNA sequence
Length = 546
Score = 91.3 bits (193), Expect(2) = 3e-026
Identities = 38/94 (40%), Positives = 60/94 (63%)
Frame = +1 / +2
Query: 43 MASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEE 222
MA+L +P + D +L+KAF+G+G + +I+IL HRD+ QR I++ Y Y EE
Sbjct: 77 MATLKVPSNVPALADDCDNLRKAFQGWGTNEALIISILGHRDAAQRRAIRKHYADTYGEE 256
Query: 223 LSHRISSELNGNHKKAMLLWILDPAGRDXTVLRE 324
L I+ E++G+ ++A++LW LDPA RD + E
Sbjct: 257 LLRSITDEISGDFERAVILWTLDPAERDAVLANE 358
Score = 46.9 bits (96), Expect(2) = 3e-026
Identities = 19/46 (41%), Positives = 28/46 (60%)
Frame = +1 / +2
Query: 364 DIICSRTPSQLXIMKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYV 501
+I CSR +QL ++Q Y+ RF LE D+ H +GD + LL+ V
Sbjct: 398 EIACSRGSAQLFAVRQAYHDRFKRSLEEDVAAHVTGDFRKLLVPLV 535
>gb|AL507314.1|AL507314 AL507314 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY01N11V 5',
mRNA sequence
Length = 568
Score = 90.9 bits (192), Expect(2) = 4e-026
Identities = 38/94 (40%), Positives = 60/94 (63%)
Frame = +1 / +3
Query: 43 MASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEYRAMYHEE 222
MA+L +P + D +L+KAF+G+G + +I+IL HRD+ QR I++ Y Y EE
Sbjct: 78 MATLKVPSNVPALADDCDNLRKAFQGWGTNEALIISILGHRDAAQRRAIRKHYADTYGEE 257
Query: 223 LSHRISSELNGNHKKAMLLWILDPAGRDXTVLRE 324
L I+ E++G+ ++A++LW LDPA RD + E
Sbjct: 258 LLRSITDEISGDFERAVILWTLDPAERDAVLAXE 359
Score = 46.9 bits (96), Expect(2) = 4e-026
Identities = 20/46 (43%), Positives = 28/46 (60%)
Frame = +1 / +3
Query: 364 DIICSRTPSQLXIMKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYV 501
+I CSR +QL ++Q Y+ RF LE D+ H +GD + LL A V
Sbjct: 399 EIACSRGSAQLFAVRQAYHDRFKRSLEEDVAAHVTGDFRKLLGALV 536
>gb|BF629785.2|BF629785 HVSMEb0013M23f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0013M23f, mRNA sequence
Length = 811
Score = 93.2 bits (197), Expect(3) = 2e-025
Identities = 43/96 (44%), Positives = 60/96 (62%)
Frame = +1 / +2
Query: 13 RSAPAQRLPAMASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQ 192
R+ + L +M S+++P +A R A+ L +A +GFG +S I+IL HRD+ QR LI
Sbjct: 65 RATHIRILLSMESVSVPAVLACARAHALALHRACEGFG*ESAREIDILAHRDATQRALIM 244
Query: 193 QEYRAMYHEELSHRISSELNGNHKKAMLLWILDPAG 300
EYR +YH +L R+S L+GNHKKAML P G
Sbjct: 245 HEYRGIYHHDLYLRLSMYLSGNHKKAMLRVGAGPRG 352
Score = 31.3 bits (62), Expect(3) = 2e-025
Identities = 13/24 (54%), Positives = 16/24 (66%)
Frame = +1 / +2
Query: 463 TSGDHQXLLLAYVGIPRYEGPEVD 534
T G L LAY+G+ +EGPEVD
Sbjct: 518 TYGHPHILPLAYLGVHGHEGPEVD 589
Score = 28.6 bits (56), Expect(3) = 2e-025
Identities = 13/32 (40%), Positives = 19/32 (59%)
Frame = +1 / +1
Query: 358 ATDIICSRTPSQLXIMKQTYYARFGTYLEHDI 453
A +ICSRT S + ++ Q Y AR L+ D+
Sbjct: 412 ARRLICSRTLSHVHMLTQAYRARSRCSLDLDL 507
>gb|BG309045.2|BG309045 HVSMEc0001K22f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0001K22f, mRNA sequence
Length = 862
Score = 67.5 bits (141), Expect(3) = 5e-025
Identities = 27/60 (45%), Positives = 40/60 (66%)
Frame = +1 / +2
Query: 145 INILTHRDSVQRGLIQQEYRAMYHEELSHRISSELNGNHKKAMLLWILDPAGRDXTVLRE 324
I+IL HRD+ QR I++ Y Y EEL I+ E++G+ ++A++LW LDPA RD + E
Sbjct: 179 ISILGHRDAAQRRAIRKHYADTYGEELLRSITDEISGDFERAVILWTLDPAERDAVLANE 358
Score = 58.3 bits (121), Expect(3) = 5e-025
Identities = 27/66 (40%), Positives = 38/66 (57%)
Frame = +1 / +2
Query: 364 DIICSRTPSQLXIMKQTYYARFGTYLEHDIGHHTSGDHQXLLLAYVGIPRYEGPEVDPTI 543
+I CSR +QL ++Q Y+ RF LE D H +GD + LL+ RYEGPEV+ +
Sbjct: 398 EIACSRGSAQLFAVRQAYHDRFKRSLEEDGPAHVTGDLRKLLVPLGSSYRYEGPEVNTRL 577
Query: 544 XTHDAK 561
+AK
Sbjct: 578 AHSEAK 595
Score = 25.8 bits (50), Expect(3) = 5e-025
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = +1 / +3
Query: 43 MASLTLPPAMANPRQDAIDLQKAFKGFG 126
MA+L +P + D +L+KAF+G+G
Sbjct: 75 MATLKVPSNVPALADDCDNLRKAFQGWG 158
>gb|BQ459986.1|BQ459986 HA07I14r HA Hordeum vulgare subsp. vulgare cDNA clone HA07I14
5-PRIME, mRNA sequence
Length = 481
Score = 95.5 bits (202), Expect(2) = 2e-024
Identities = 40/101 (39%), Positives = 62/101 (61%)
Frame = +1 / +2
Query: 22 PAQRLPAMASLTLPPAMANPRQDAIDLQKAFKGFGCDSTTVINILTHRDSVQRGLIQQEY 201
P + P MA+L++P A+ +D L+KAF G+G + +I+IL HRD+ QR I++ Y
Sbjct: 53 PDRTGPEMATLSVPAAVPAVAEDCEQLRKAFAGWGTNERLIISILAHRDAAQRRAIRKHY 232
Query: 202 RAMYHEELSHRISSELNGNHKKAMLLWILDPAGRDXTVLRE 324
Y EEL I+ E++G ++A++ W LDPA RD + E
Sbjct: 233 ADAYGEELLRSITDEIHGKFERAVIQWTLDPAERDAVLANE 355
Score = 36.8 bits (74), Expect(2) = 2e-024
Identities = 15/31 (48%), Positives = 19/31 (61%)
Frame = +1 / +1
Query: 352 RAATDIICSRTPSQLXIMKQTYYARFGTYLE 444
RA +I C+RTP+ L KQ Y+ RF LE
Sbjct: 385 RALVEIACARTPAHLFAAKQAYHDRFKRSLE 477
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 285,446,302
Number of Sequences: 312970
Number of extensions: 3925611
Number of successful extensions: 34139
Number of sequences better than 1.0e-020: 29
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7465340784
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)