TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAZ4d09.yg.2.1
(542 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM371229.2|BM371229 EBro04_SQ003_O11_R root, 3 week, sal... 216 6e-056
gb|BM373233.2|BM373233 EBma04_SQ003_K04_R maternal, 10 DPA,... 213 5e-055
gb|BQ767981.1|BQ767981 EBro08_SQ010_J07_R root, 3 week, dro... 208 2e-053
gb|BM097354.2|BM097354 EBro01_SQ004_O08_R root, 3 week, hyd... 191 3e-048
gb|BF260721.2|BF260721 HVSMEf0022M07f Hordeum vulgare seedl... 179 9e-045
gb|BQ758396.1|BQ758396 EBma05_SQ001_L05_R maternal, 12 DPA,... 156 7e-038
gb|BI955333.1|BI955333 HVSMEm0022J13f Hordeum vulgare green... 136 8e-032
gb|CA014589.1|CA014589 HT11M09r HT Hordeum vulgare subsp. v... 126 9e-029
gb|BF623053.2|BF623053 HVSMEa0012M22f Hordeum vulgare seedl... 121 4e-027
gb|BF628008.2|BF628008 HVSMEb0002J11f Hordeum vulgare seedl... 116 9e-026
gb|BQ763476.1|BQ763476 EBro02_SQ008_B17_R root, 3 week, hyd... 116 1e-025
gb|AL507983.1|AL507983 AL507983 Hordeum vulgare Barke devel... 102 2e-021
gb|BF259970.1|BF259970 HVSMEf0020M03f Hordeum vulgare seedl... 101 2e-021
>gb|BM371229.2|BM371229 EBro04_SQ003_O11_R root, 3 week, salt-stressed, cv Optic, EBro04
Hordeum vulgare subsp. vulgare cDNA clone
EBro04_SQ003_O11 5', mRNA sequence
Length = 597
Score = 216 bits (467), Expect = 6e-056
Identities = 91/143 (63%), Positives = 113/143 (79%)
Frame = +1 / +1
Query: 73 PYMAMVFLQFGFAGMFLISVASLRQGMSHYVLVVYRNVVAAIVMAPFALWFERKTRPKMS 252
PY+AMV LQ GFAGM++I+VASL++GMSH+VLVVYRN+VA VMAPFAL+FER RPKM+
Sbjct: 169 PYLAMVLLQVGFAGMYIIAVASLKRGMSHFVLVVYRNLVATAVMAPFALYFERGLRPKMT 348
Query: 253 LPVFIKILALGLLEPVLDQNFIYMGVNSTSASFSSALTNILPALTFVNAIILRMERIVIX 432
+ +FIKI+ L LEPVLDQN YMG N TSA F++AL NILPA+TFV A+ILRME++ +
Sbjct: 349 ITIFIKIMGLAFLEPVLDQNLYYMGANLTSAGFATALVNILPAVTFVLALILRMEKVRLR 528
Query: 433 XRRSQAXXXGTAXTVXXXLLMIL 501
SQA GT TV +LM+L
Sbjct: 529 SLHSQAKIAGTVLTVAGAVLMVL 597
Score = 103 bits (219), Expect = 9e-022
Identities = 58/142 (40%), Positives = 65/142 (45%)
Frame = -1 / -2
Query: 494 ISKXXXTVXAVPXXXAWXXXXXXXXXXXXSMMAFTKVRAGNMLVNAEEKLADVLFTPM*M 315
IS TV VP AW M A TKV AG ML +A A+V F PM*
Sbjct: 590 ISTAPATVRTVPAILAWLCRLRSRTFSMRRMSANTKVTAGRMLTSAVANPAEVRFAPM** 411
Query: 314 KFWSSTGSSNPKARILMNTGRLILGLVFLSNHSANGXXXXXXXXXXXXXXX*WLIPCRRD 135
+FWSSTGS N ILM +I GL LS A G WLIP RD
Sbjct: 410 RFWSSTGSRNASPMILMKIVIVIFGLSPLSK*RAKGAMTAVATRLR*TTRTKWLIPRLRD 231
Query: 134 ATEMRNIPAKPN*RNTMAM*GI 69
AT M IPA P +T+A G+
Sbjct: 230 ATAMMYIPANPTCSSTIARYGL 165
>gb|BM373233.2|BM373233 EBma04_SQ003_K04_R maternal, 10 DPA, no treatment, cv Optic, EBma04
Hordeum vulgare subsp. vulgare cDNA clone
EBma04_SQ003_K04 5', mRNA sequence
Length = 577
Score = 213 bits (460), Expect = 5e-055
Identities = 91/151 (60%), Positives = 114/151 (75%)
Frame = +1 / +1
Query: 67 VMPYMAMVFLQFGFAGMFLISVASLRQGMSHYVLVVYRNVVAAIVMAPFALWFERKTRPK 246
V PYM M+ LQ G+AGM+++SVASL++GMSHYVLV YRN+VA +VM PFAL FE+ RPK
Sbjct: 10 VKPYMMMILLQIGYAGMYIVSVASLKRGMSHYVLVTYRNLVATLVMLPFALVFEKGVRPK 189
Query: 247 MSLPVFIKILALGLLEPVLDQNFIYMGVNSTSASFSSALTNILPALTFVNAIILRMERIV 426
M+L +FIKIL L LLEPVLDQN Y+G TSASFSSAL NILPA+TF+ AI+LRME++
Sbjct: 190 MTLRIFIKILGLALLEPVLDQNLYYVGNKLTSASFSSALVNILPAVTFIMAIVLRMEKLR 369
Query: 427 IXXRRSQAXXXGTAXTVXXXLLMILXXXXII 519
+ SQA GT TV +LMI+ ++
Sbjct: 370 LRSSHSQAKVAGTICTVIGAVLMIMYHGPVV 462
Score = 100 bits (212), Expect = 9e-021
Identities = 57/144 (39%), Positives = 67/144 (46%)
Frame = -1 / -3
Query: 497 IISKXXXTVXAVPXXXAWXXXXXXXXXXXXSMMAFTKVRAGNMLVNAEEKLADVLFTPM* 318
II TV VP AW S MA KV AG ML +A+EKLA+V P *
Sbjct: 440 IIRTAPMTVQMVPATLAWLCELRSRSFSIRSTMAMMKVTAGRMLTSADEKLAEVSLLPT* 261
Query: 317 MKFWSSTGSSNPKARILMNTGRLILGLVFLSNHSANGXXXXXXXXXXXXXXX*WLIPCRR 138
+FWSSTGSS+ +ILM +ILGL S A G *WL+P
Sbjct: 260 YRFWSSTGSSSASPKILMKMRSVILGLTPFSKTRAKGSMTSVATRLR*VTRT*WLMPRLS 81
Query: 137 DATEMRNIPAKPN*RNTMAM*GIT 66
+ATE IPA P + M G T
Sbjct: 80 EATETMYIPAYPICSRIIIMYGFT 9
>gb|BQ767981.1|BQ767981 EBro08_SQ010_J07_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ010_J07 5', mRNA sequence
Length = 611
Score = 208 bits (449), Expect = 2e-053
Identities = 87/145 (60%), Positives = 111/145 (76%)
Frame = +1 / +3
Query: 67 VMPYMAMVFLQFGFAGMFLISVASLRQGMSHYVLVVYRNVVAAIVMAPFALWFERKTRPK 246
V PY+AMV LQ GFAGM++I+VASL+ GMSH+VLVVYRN+VA VM PFA++FER RPK
Sbjct: 177 VKPYLAMVLLQVGFAGMYIIAVASLKAGMSHFVLVVYRNLVATAVMMPFAIYFERGLRPK 356
Query: 247 MSLPVFIKILALGLLEPVLDQNFIYMGVNSTSASFSSALTNILPALTFVNAIILRMERIV 426
M++ +FIKI+ L LEPV+DQN +MG TSA F++AL NILPA+TFV A+ILRME++
Sbjct: 357 MTITIFIKIMGLAFLEPVIDQNLYFMGAKMTSAGFATALVNILPAVTFVLALILRMEKVR 536
Query: 427 IXXRRSQAXXXGTAXTVXXXLLMIL 501
+ SQA GT TV +LM+L
Sbjct: 537 LRSLHSQAKIAGTLLTVAGAVLMVL 611
Score = 104 bits (222), Expect = 4e-022
Identities = 57/143 (39%), Positives = 65/143 (45%)
Frame = -1 / -2
Query: 494 ISKXXXTVXAVPXXXAWXXXXXXXXXXXXSMMAFTKVRAGNMLVNAEEKLADVLFTPM*M 315
IS TV +VP AW M A TKV AG ML +A A+V+F PM
Sbjct: 604 ISTAPATVRSVPAILAWLWRLRSRTFSMRRMRASTKVTAGRMLTSAVANPAEVIFAPMKY 425
Query: 314 KFWSSTGSSNPKARILMNTGRLILGLVFLSNHSANGXXXXXXXXXXXXXXX*WLIPCRRD 135
+FWS TGS N ILM +I GL LS A G WLIP RD
Sbjct: 424 RFWSITGSRNASPMILMKMVIVIFGLSPLSK*MAKGIMTAVATRLR*TTRTKWLIPALRD 245
Query: 134 ATEMRNIPAKPN*RNTMAM*GIT 66
AT M IPA P +T+A G T
Sbjct: 244 ATAMMYIPANPTCSSTIARYGFT 176
>gb|BM097354.2|BM097354 EBro01_SQ004_O08_R root, 3 week, hydroponic grown, no treatment, cv
Optic, EBro01 Hordeum vulgare subsp. vulgare cDNA clone
EBro01_SQ004_O08 5', mRNA sequence
Length = 534
Score = 191 bits (411), Expect = 3e-048
Identities = 80/116 (68%), Positives = 96/116 (82%)
Frame = +1 / +2
Query: 67 VMPYMAMVFLQFGFAGMFLISVASLRQGMSHYVLVVYRNVVAAIVMAPFALWFERKTRPK 246
V PYM M+ LQ G+AGM+++SVASL++GMSHYVLV YRN+VA +VM PFAL FE+ RPK
Sbjct: 185 VKPYMMMILLQIGYAGMYIVSVASLKRGMSHYVLVTYRNLVATLVMLPFALVFEKGVRPK 364
Query: 247 MSLPVFIKILALGLLEPVLDQNFIYMGVNSTSASFSSALTNILPALTFVNAIILRM 414
M+L +FIKIL L LLEPVLDQN Y+G TSASFSSAL NILPA+TF+ AI+LRM
Sbjct: 365 MTLRIFIKILGLALLEPVLDQNLYYVGNKLTSASFSSALVNILPAVTFIMAIVLRM 532
>gb|BF260721.2|BF260721 HVSMEf0022M07f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0022M07f, mRNA sequence
Length = 542
Score = 179 bits (386), Expect = 9e-045
Identities = 74/119 (62%), Positives = 93/119 (78%)
Frame = +1 / +2
Query: 67 VMPYMAMVFLQFGFAGMFLISVASLRQGMSHYVLVVYRNVVAAIVMAPFALWFERKTRPK 246
V PY+AMV LQ GFAGM++I+VASL GMSH+VLVVYRN+V VM PF ++F R RPK
Sbjct: 170 VKPYLAMVLLQVGFAGMYIIAVASLMAGMSHFVLVVYRNLVGTAVMMPFVIYFVRGLRPK 349
Query: 247 MSLPVFIKILALGLLEPVLDQNFIYMGVNSTSASFSSALTNILPALTFVNAIILRMERI 423
M++ +FIKI+ L LEPV+DQN +MG TSA F++AL NILPA+TFV A+ILRME +
Sbjct: 350 MTITMFIKIMGLAFLEPVIDQNLYFMGAKMTSAGFATALVNILPAVTFVLALILRMENV 526
>gb|BQ758396.1|BQ758396 EBma05_SQ001_L05_R maternal, 12 DPA, no treatment, cv Optic, EBma05
Hordeum vulgare subsp. vulgare cDNA clone
EBma05_SQ001_L05 5', mRNA sequence
Length = 472
Score = 156 bits (336), Expect = 7e-038
Identities = 65/96 (67%), Positives = 78/96 (81%)
Frame = +1 / +3
Query: 67 VMPYMAMVFLQFGFAGMFLISVASLRQGMSHYVLVVYRNVVAAIVMAPFALWFERKTRPK 246
V PYM M+ LQ G+AGM+++SVASL++GMSHYVLV YRN+VA +VM PFAL FE+ RPK
Sbjct: 183 VKPYMMMILLQIGYAGMYIVSVASLKRGMSHYVLVTYRNLVATLVMLPFALVFEKGVRPK 362
Query: 247 MSLPVFIKILALGLLEPVLDQNFIYMGVNSTSASFS 354
M+L +FIKIL L LLEPVLDQN Y+G TSASFS
Sbjct: 363 MTLRIFIKILGLALLEPVLDQNLYYVGNKLTSASFS 470
>gb|BI955333.1|BI955333 HVSMEm0022J13f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0022J13f, mRNA sequence
Length = 659
Score = 136 bits (292), Expect = 8e-032
Identities = 61/109 (55%), Positives = 75/109 (68%)
Frame = +1 / +2
Query: 67 VMPYMAMVFLQFGFAGMFLISVASLRQGMSHYVLVVYRNVVAAIVMAPFALWFERKTRPK 246
V PYM M+ LQ G+AGM+++SVASL++GMSHYVLV YRN+VA +VM PFAL FE+ RPK
Sbjct: 278 VKPYMMMILLQIGYAGMYIVSVASLKRGMSHYVLVTYRNLVATLVMLPFALVFEKGVRPK 457
Query: 247 MSLPVFIKILALGLLEPVLDQNFIYMGVNSTSASFSSALTNILPALTFV 393
M+L +FIKIL L + G TS FSSAL NIL A T +
Sbjct: 458 MTLRIFIKILGWRCSSLCLTRTCTTWGTKLTSGHFSSALGNILAAGTLI 604
>gb|CA014589.1|CA014589 HT11M09r HT Hordeum vulgare subsp. vulgare cDNA clone HT11M09
5-PRIME, mRNA sequence
Length = 671
Score = 126 bits (270), Expect = 9e-029
Identities = 57/151 (37%), Positives = 93/151 (61%)
Frame = +1 / +1
Query: 43 ELAGTWRKVMPYMAMVFLQFGFAGMFLISVASLRQGMSHYVLVVYRNVVAAIVMAPFALW 222
++A ++ +P +AMV +Q GFAGM ++S +L GMS YVL+ YRN++A + +AP A +
Sbjct: 118 QIAAMGKECLPTVAMVLVQLGFAGMNVLSKLALDTGMSPYVLIAYRNLIAGMFLAPGAYY 297
Query: 223 FERKTRPKMSLPVFIKILALGLLEPVLDQNFIYMGVNSTSASFSSALTNILPALTFVNAI 402
FER++ ++ V I+I + L+Q ++G+ ST+ + + AL+N LPALTFV A
Sbjct: 298 FERRSGMVITKQVLIQIFFSSIFGATLNQVLYFVGLKSTTPTVACALSNTLPALTFVMAA 477
Query: 403 ILRMERIVIXXRRSQAXXXGTAXTVXXXLLM 495
IL+ME + + +A GT V ++M
Sbjct: 478 ILKMETVRLKTSSGKAKVLGTVVCVGGSMVM 570
>gb|BF623053.2|BF623053 HVSMEa0012M22f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0012M22f, mRNA sequence
Length = 833
Score = 121 bits (258), Expect = 4e-027
Identities = 52/146 (35%), Positives = 89/146 (60%)
Frame = +1 / +2
Query: 64 KVMPYMAMVFLQFGFAGMFLISVASLRQGMSHYVLVVYRNVVAAIVMAPFALWFERKTRP 243
KV +M ++ LQF AG ++S A+L G+S V +VYRNV++ ++APFA + E+K RP
Sbjct: 122 KVKLFMGVLALQFLLAGFHIVSRAALNMGISKLVFIVYRNVISLALLAPFAYFLEKKDRP 301
Query: 244 KMSLPVFIKILALGLLEPVLDQNFIYMGVNSTSASFSSALTNILPALTFVNAIILRMERI 423
++L + + L L+ +Q F +G+ S +++SA+ N++PA+TFV A +LR+E++
Sbjct: 302 PLTLSLLAEFFVLALIGITANQGFYLLGLYHLSPTYASAIQNMVPAITFVLAAVLRLEQV 481
Query: 424 VIXXRRSQAXXXGTAXTVXXXLLMIL 501
+ R A GT +V ++ L
Sbjct: 482 DLGRRHGVAKVVGTVVSVGGATVITL 559
>gb|BF628008.2|BF628008 HVSMEb0002J11f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0002J11f, mRNA sequence
Length = 665
Score = 116 bits (248), Expect = 9e-026
Identities = 52/146 (35%), Positives = 87/146 (59%)
Frame = +1 / +3
Query: 64 KVMPYMAMVFLQFGFAGMFLISVASLRQGMSHYVLVVYRNVVAAIVMAPFALWFERKTRP 243
KV +M ++ LQF AG ++S A+L G+S V +VYRNV++ ++APFA + E+K RP
Sbjct: 42 KVKLFMGVLALQFLLAGFHIVSRAALNMGISKLVFIVYRNVISLALLAPFAYFLEKKDRP 221
Query: 244 KMSLPVFIKILALGLLEPVLDQNFIYMGVNSTSASFSSALTNILPALTFVNAIILRMERI 423
++L + + L L+ +Q F +G+ S +++SA+ N+ PA+TFV A LR+E++
Sbjct: 222 PLTLSLLAEFFVLALIGITANQGFYLLGLYHLSPTYASAIQNMEPAITFVLAAELRLEQV 401
Query: 424 VIXXRRSQAXXXGTAXTVXXXLLMIL 501
+ R A GT +V ++ L
Sbjct: 402 DLGRRHGVANVVGTVVSVGGATVITL 479
>gb|BQ763476.1|BQ763476 EBro02_SQ008_B17_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ008_B17 5', mRNA sequence
Length = 536
Score = 116 bits (247), Expect = 1e-025
Identities = 49/135 (36%), Positives = 83/135 (61%)
Frame = +1 / +3
Query: 34 DRMELAGTWRKVMPYMAMVFLQFGFAGMFLISVASLRQGMSHYVLVVYRNVVAAIVMAPF 213
D + G K ++AM+ LQFG+AG ++S +L G+S V VYRN++A ++ PF
Sbjct: 78 DARRVCGMPEKAQLHVAMLALQFGYAGFHVVSRLALNMGISKLVFPVYRNIIALFLLVPF 257
Query: 214 ALWFERKTRPKMSLPVFIKILALGLLEPVLDQNFIYMGVNSTSASFSSALTNILPALTFV 393
A + E+K RP+++L ++ L L +Q F +G+++TS +F+SA+ N +PA+TF
Sbjct: 258 AYFLEKKDRPRLTLGFAVQFFFLALCGITANQGFYLLGLDNTSPTFASAIQNSVPAITFG 437
Query: 394 NAIILRMERIVIXXR 438
A LR+E++ + R
Sbjct: 438 MAAALRIEKVRLDRR 482
>gb|AL507983.1|AL507983 AL507983 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY07G20V 5',
mRNA sequence
Length = 700
Score = 102 bits (217), Expect = 2e-021
Identities = 44/141 (31%), Positives = 74/141 (52%)
Frame = +1 / +3
Query: 73 PYMAMVFLQFGFAGMFLISVASLRQGMSHYVLVVYRNVVAAIVMAPFALWFERKTRPKMS 252
PY+ V +Q + GMF++S A+ QG + YV + YR A++++ P A+ FERK MS
Sbjct: 93 PYVIAVIIQVIYTGMFVVSKAAFNQGFNTYVFIFYRQAAASLLLLPLAIAFERKNARAMS 272
Query: 253 LPVFIKILALGLLEPVLDQNFIYMGVNSTSASFSSALTNILPALTFVNAIILRMERIVIX 432
+ +K+ L+ N + + TSA+ SA +N +P +TF A++LRME + +
Sbjct: 273 FRLLLKLFLFALIGTTFSLNLYNVSLKLTSATVGSATSNSMPVVTFFIALLLRMEVVKLR 452
Query: 433 XRRSQAXXXGTAXTVXXXLLM 495
A G + L++
Sbjct: 453 SPSGMAKVAGVGLCLAGVLVI 515
>gb|BF259970.1|BF259970 HVSMEf0020M03f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0020M03f, mRNA sequence
Length = 549
Score = 101 bits (216), Expect = 2e-021
Identities = 45/103 (43%), Positives = 62/103 (60%)
Frame = +1 / +2
Query: 232 KTRPKMSLPVFIKILALGLLEPVLDQNFIYMGVNSTSASFSSALTNILPALTFVNAIILR 411
K RP+M+ VF+ I L L+ PV+DQNF Y G+ TS +FS A++N+LPA+TFV A+I R
Sbjct: 23 KVRPRMTPLVFLNIFLLALMGPVIDQNFYYAGLKYTSPTFSCAMSNMLPAMTFVMAVIFR 202
Query: 412 MERIVIXXRRSQAXXXGTAXTVXXXLLMILXXXXIIXXXXSXH 540
ME++ + R A GT TV +LM L + S H
Sbjct: 203 MEKVDLKKARCLAKVAGTLVTVAGAMLMTLYKGRAVQMVWSRH 331
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 247,667,724
Number of Sequences: 312970
Number of extensions: 3842771
Number of successful extensions: 69232
Number of sequences better than 1.0e-020: 14
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 5554858270
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)