TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAZ3f05.yg.3.1
(1626 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU985234.1|BU985234 HF06I14r HF Hordeum vulgare subsp. v... 438 e-122
gb|BF064493.2|BF064493 HV_CEb0015G10f Hordeum vulgare seedl... 424 e-118
gb|BQ469437.1|BQ469437 HM04L06r HM Hordeum vulgare subsp. v... 207 e-102
gb|BQ659809.1|BQ659809 HF01M14w HF Hordeum vulgare subsp. v... 363 2e-099
gb|CA017868.1|CA017868 HV06J10r HV Hordeum vulgare subsp. v... 360 1e-098
gb|AV920015.1|AV920015 AV920015 K. Sato unpublished cDNA li... 336 3e-091
gb|AV917216.1|AV917216 AV917216 K. Sato unpublished cDNA li... 319 4e-089
gb|BQ661666.1|BQ661666 HM04L06u HM Hordeum vulgare subsp. v... 278 5e-074
gb|CV054336.1|CV054336 BNEL109d2 Barley EST endosperm libra... 149 2e-048
gb|AV833861.1|AV833861 AV833861 K. Sato unpublished cDNA li... 116 2e-044
gb|AV834608.1|AV834608 AV834608 K. Sato unpublished cDNA li... 153 3e-043
gb|BQ664507.1|BQ664507 HV03K06u HV Hordeum vulgare subsp. v... 175 5e-043
gb|BI951455.1|BI951455 HVSMEl0025P22f Hordeum vulgare spike... 155 9e-037
>gb|BU985234.1|BU985234 HF06I14r HF Hordeum vulgare subsp. vulgare cDNA clone HF06I14
5-PRIME, mRNA sequence
Length = 615
Score = 438 bits (950), Expect = e-122
Identities = 172/201 (85%), Positives = 194/201 (96%)
Frame = +3 / +3
Query: 408 EFIVGPIPVDDGNGKEVATEIVTNMVTNKTFYTDSSGRDFIKRIRDYRSEWKIEVHQPIA 587
EFIVGPIPVDD NGKE++TEI+T+M TNKTFYTDSSGRDFIKRIRDYRSEWKIEV+QP+A
Sbjct: 12 EFIVGPIPVDDENGKELSTEIITSMATNKTFYTDSSGRDFIKRIRDYRSEWKIEVNQPVA 191
Query: 588 GNYYPVNLGIYVEDGSKELSVLVDRSIGGSSIKDGQIELMLHRRLLHDDGKGVAEALNET 767
GNYYP+NLGIYVEDG+KELS+LVDRS+GGSSIKDGQIELMLHRRLL+DDG+GVAEAL+E
Sbjct: 192 GNYYPINLGIYVEDGNKELSILVDRSVGGSSIKDGQIELMLHRRLLNDDGRGVAEALDEK 371
Query: 768 VCLDKQCEGLIIQGKYYVKIDPQGEGARWRRTFGQEIYSPLLLAFTEQDGGNWANSHVAK 947
VCLD QCEGL+I+GKYY+KIDPQG+GARWRRTFGQE+YSPLLLAF EQDGGNWANSHV+
Sbjct: 372 VCLDDQCEGLVIEGKYYLKIDPQGDGARWRRTFGQELYSPLLLAFAEQDGGNWANSHVSS 551
Query: 948 FSAMDSTYSLPDNVAMLTLQE 1010
FSAMD TYSLP+NVA+LTL+E
Sbjct: 552 FSAMDPTYSLPENVALLTLEE 614
Score = 314 bits (680), Expect = 9e-085
Identities = 141/201 (70%), Positives = 160/201 (79%)
Frame = -2 / -2
Query: 1007 LKGKHCNIIRQAVS*IHRREFCNM*IRPVASILLCESKKERGVYFLAKCPTPPSSFSLWV 828
LK K +I R+ VS IH RE NM*IRPVASILLCE KK+RGV FL KC TPPS +LWV
Sbjct: 611 LKRKQRDIFRKTVSWIHCRER*NM*IRPVASILLCERKKKRGVQFLTKCTTPPSPVTLWV 432
Query: 827 YFDIVFSLYNESFTLLIETDCFI*RLGDAFPIIME*PPV*H*LYLSILYTGTSN*PVYQY 648
F+IVFS N SFTL+I+TD FI* + II+E*PPV*H*LYLSILY TSN PVY+Y
Sbjct: 431 NFEIVFSFNN*SFTLIIQTDFFI*CFRNTTTIIIE*PPV*H*LYLSILYARTSNRPVYKY 252
Query: 647 RQLFAAILHIDAKINRIIVSSNRLVHFNLPLRSIISYPFDEITSTGISVECFVCHHVCNN 468
RQLF AI HI+AKINRIIVSSNRLV+FNLPLR+II YPFDEITSTGISV+ FVC H N+
Sbjct: 251 RQLFVAIFHINAKINRIIVSSNRLVYFNLPLRAIIPYPFDEITSTGISVKRFVCCHASND 72
Query: 467 FSCYFFSISIIYWYGPNYKFS 405
FS FFSI ++YWY PNY+ +
Sbjct: 71 FS*KFFSIFVVYWYWPNYELT 9
Score = 308 bits (666), Expect = 7e-083
Identities = 137/201 (68%), Positives = 151/201 (75%)
Frame = +2 / +2
Query: 407 *IYSWAHTSR*WKWKRSSN*NCYKHGDKQNILH*FQWT*FHQKDKRLSIGVED*SAPAYC 586
*++SWA+TSR KWKR+ N*N Y HG+KQN+LH*FQWT*FHQKD LS+GVED*S P C
Sbjct: 11 *VHSWANTSRRRKWKRTFN*NHY*HGNKQNVLH*FQWT*FHQKDTGLSLGVED*SKPTCC 190
Query: 587 WKLLSC*SWHLCGGWQQRAVCTGRQVNWRFQYKGWTNRANATQEATP**WERRRRGAK*N 766
WKLLS *SWHLCG WQQR V T RQV WRF++KGWTNRANATQEAT **W +*
Sbjct: 191 WKLLSY*SWHLCGRWQQRVVYTCRQVCWRFEHKGWTNRANATQEATQ**WSWCCGSTR*K 370
Query: 767 SLSR*AM*RTHYTRKILCQNRPTRRRSSVASDIWPGNILPSPSCFHRAGWRQLGEFTCCK 946
SL *+M*RT Y RKIL QN PTR R VAS IW G +LPS SC RAGWRQLGEFTC
Sbjct: 371 SLFG*SM*RTSY*RKILSQN*PTR*RG*VASYIWSGTVLPSSSCVRRAGWRQLGEFTCFI 550
Query: 947 ILCDGFNLQLA**CCNAYPSG 1009
LC+G NLQ +* C AY G
Sbjct: 551 FLCNGSNLQSS*KCRVAYA*G 613
Score = 272 bits (589), Expect = 3e-072
Identities = 120/177 (67%), Positives = 132/177 (74%)
Frame = -1 / -1
Query: 1008 PEG*ALQHYQASCKLNPSQRILQHVNSPSCLHPAL*KQEGEGSIFPGQMSDATELLLLVG 829
P+ *A +H+Q CKL+P QR ++HVNSPSCLHPAL QE EGS P QM DAT LVG
Sbjct: 612 PQA*ATRHFQEDCKLDPLQRKMKHVNSPSCLHPALRTQEEEGSTVPDQMYDAT*PRHLVG 433
Query: 828 LF*HSIFLV**VLHIAYRDRLFHLAPRRRLSHHHGVASCVALALFVHPLYWNLQLTCLPV 649
F* SIFL * VLHI + +RLFHL + HHH VASCVALALFVHPL NLQ TCL V
Sbjct: 432 QF*DSIFLQ*LVLHIDHPNRLFHLVLPQHHDHHH*VASCVALALFVHPLCSNLQQTCLQV 253
Query: 648 QTALCCHPPHRCQD*QDNSFQQ*AGALQSSTPIDNLLSF**NHVHWNQCRMFCLSPC 478
T LCCH PH+CQD* DNSFQQ G LQSSTP DN +SF**NHVHWNQC+ FCL PC
Sbjct: 252 *TTLCCHLPHKCQD**DNSFQQQVGLLQSSTPSDNPVSF**NHVHWNQCKTFCLLPC 82
Score = 234 bits (505), Expect = 1e-060
Identities = 115/177 (64%), Positives = 124/177 (70%)
Frame = +1 / +1
Query: 481 W*QTKHSTLIPVDVISSKG*EIIDRSGRLKCTSXXXXXXXXXXXASMWRMAAKSCLYW*T 660
W QTK TLIPVDVISSKG II RSGRLK T+ A MW+MA KSCLY *T
Sbjct: 85 WQQTKRFTLIPVDVISSKGYGIIARSGRLK*TNLLLETIILLILAFMWKMATKSCLYL*T 264
Query: 661 GQLEVPV*RMDK*S*CYTGGYSMMMGKASPRR*MKQSVSISNVKDSLYKENTMSK*THKE 840
G LEV *RMDK*S*CYTGGYSMMM + *MK+SV + NVKD L KENT+SK THK
Sbjct: 265 GLLEVRA*RMDK*S*CYTGGYSMMMVVVLRKH*MKKSVWMINVKD*LLKENTISKLTHKV 444
Query: 841 KELGGVGHLARKYTPLSFLLSQSRMEATGRIHMLQNSLRWIQLTACLIMLQCLPFRS 1011
LGGV HL R TPL FL SQSRMEATGRIHM SL+WIQLT L M +CL R+
Sbjct: 445 TGLGGVVHLVRNCTPLFFLRSQSRMEATGRIHMFHLSLQWIQLTVFLKMSRCLRLRN 615
Score = 132 bits (282), Expect(2) = 3e-058
Identities = 58/75 (77%), Positives = 60/75 (80%)
Frame = -3 / -3
Query: 991 ATLSGKL*VESIAENFATCEFAQLPPSCSVKARRRGEYISWPNVRRHRAPSPCGSILT*Y 812
AT SG+L*V SIAE TCEFAQLPPSCS ARRRGEY S PNVRRH APSPCGSIL *Y
Sbjct: 595 ATFSGRL*VGSIAEKDETCEFAQLPPSCSANARRRGEYSS*PNVRRHLAPSPCGSILR*Y 416
Query: 811 FPCIMSPSHCLSRQT 767
FP I SPSH S+QT
Sbjct: 415 FPSITSPSH*SSKQT 371
Score = 117 bits (251), Expect(2) = 3e-058
Identities = 53/65 (81%), Positives = 57/65 (87%)
Frame = -3 / -3
Query: 601 G**FPAIGWCTSIFHSDR*SLILLMKSRPLESV*NVLFVTMFVTISVATSFPFPSSTGMG 422
G**FPA GW TSIFHS+R*S ILLMKSRPLESV*NVLFV M V ISV +SFPF SSTG+G
Sbjct: 205 G**FPATGWFTSIFHSER*SRILLMKSRPLESV*NVLFVAMLVMISVESSFPFSSSTGIG 26
Query: 421 PTINS 407
PT+NS
Sbjct: 25 PTMNS 11
>gb|BF064493.2|BF064493 HV_CEb0015G10f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0015G10f, mRNA sequence
Length = 806
Score = 424 bits (921), Expect = e-118
Identities = 174/227 (76%), Positives = 199/227 (87%)
Frame = +3 / +2
Query: 444 NGKEVATEIVTNMVTNKTFYTDSSGRDFIKRIRDYRSEWKIEVHQPIAGNYYPVNLGIYV 623
NGKE++TEI+T+M TNKTFYTDSSGRDFIKRIRDYRSEWKIEV+QP+AGNYYP+NLGIYV
Sbjct: 5 NGKELSTEIITSMATNKTFYTDSSGRDFIKRIRDYRSEWKIEVNQPVAGNYYPINLGIYV 184
Query: 624 EDGSKELSVLVDRSIGGSSIKDGQIELMLHRRLLHDDGKGVAEALNETVCLDKQCEGLII 803
EDG+KELS+L DRS+GGSSIKDGQI LMLH RLL+DDG+GVAEALNE VC D QCEGL+I
Sbjct: 185 EDGNKELSILADRSVGGSSIKDGQI*LMLHMRLLNDDGRGVAEALNEKVCSDDQCEGLLI 364
Query: 804 QGKYYVKIDPQGEGARWRRTFGQEIYSPLLLAFTEQDGGNWANSHVAKFSAMDSTYSLPD 983
+GKYY+KIDPQG+GARWR T GQE+YSPLLLAF+EQDGGNW NSHV+ SAM+ TYSLP
Sbjct: 365 EGKYYLKIDPQGDGARWRPTLGQELYSPLLLAFSEQDGGNWPNSHVSFLSAMEPTYSLPV 544
Query: 984 NVAMLTLQELEDGSVLLRFAHLYEAGEDKDLSSLASIDLKRVFPEKK 1124
+LTL+ELEDGSVLLR AHLYEA + KDLS+LA + FP K
Sbjct: 545 IGTLLTLEELEDGSVLLRLAHLYEAWKHKDLSTLAIAYPQEGFPRIK 685
Score = 299 bits (647), Expect(2) = 1e-081
Identities = 144/220 (65%), Positives = 163/220 (74%)
Frame = -2 / -2
Query: 1103 LEVDTR*RRKILVLPGLI*MSKPKENTSIFKLLKGKHCNIIRQAVS*IHRREFCNM*IRP 924
L V R +IL+ P L+*+S+PKENTS+FK LK K R+ VS H RE NM*IRP
Sbjct: 664 LRVSNRQS*EILMFPSLV*VSQPKENTSVFKFLKRKQRTNYRKTVSWFHCREK*NM*IRP 485
Query: 923 VASILLCESKKERGVYFLAKCPTPPSSFSLWVYFDIVFSLYNESFTLLIETDCFI*RLGD 744
VASILL E KK+RGV FL +C TPPS +LWV F IVFS SFTL+I TD FI* +
Sbjct: 484 VASILL*ERKKKRGVQFLTQCRTPPSPVTLWVNFKIVFSFNK*SFTLIIRTDFFI*CFRN 305
Query: 743 AFPIIME*PPV*H*LYLSILYTGTSN*PVYQYRQLFAAILHIDAKINRIIVSSNRLVHFN 564
II+E*P V*H LYLSILY TSN PV +YRQLF AI HI+AKINRIIVSSNRLV+FN
Sbjct: 304 TTTIIIE*PHV*HKLYLSILYARTSNRPVCKYRQLFVAIFHINAKINRIIVSSNRLVYFN 125
Query: 563 LPLRSIISYPFDEITSTGISVECFVCHHVCNNFSCYFFSI 444
LPLR+II YPFDEITSTGISV+ FVC H N+FS FFSI
Sbjct: 124 LPLRAIIPYPFDEITSTGISVKRFVCCHASNDFS*KFFSI 5
Score = 275 bits (595), Expect = 5e-073
Identities = 127/207 (61%), Positives = 141/207 (68%)
Frame = -1 / -1
Query: 1098 GRYSLKTKDPCPPRPHIDEQTEGEHFHLQAPEG*ALQHYQASCKLNPSQRILQHVNSPSC 919
G+ S + +DP +P I E TEGEHF LQ P+ *A Q CKL P QR ++HVN SC
Sbjct: 659 GKQSPELRDPYVSKPRIGEPTEGEHFRLQVPQA*ATYQLQEDCKLVPLQREMKHVN*ASC 480
Query: 918 LHPAL*KQEGEGSIFPGQMSDATELLLLVGLF*HSIFLV**VLHIAYRDRLFHLAPRRRL 739
LH AL QE EGS M DAT LVG F* SIFL * VLHI + +RLFHL +
Sbjct: 479 LHLALRTQEEEGSTVLDPM*DAT*PRHLVGQF*DSIFLQ*VVLHIDHPNRLFHLVLPQHH 300
Query: 738 SHHHGVASCVALALFVHPLYWNLQLTCLPVQTALCCHPPHRCQD*QDNSFQQ*AGALQSS 559
HHH VASCVA A+FVHPL NLQ TCL V T LCCH PH+CQD* DNSFQQ G LQSS
Sbjct: 299 DHHH*VASCVA*AIFVHPLCSNLQQTCLQV*TTLCCHLPHKCQD**DNSFQQQVGLLQSS 120
Query: 558 TPIDNLLSF**NHVHWNQCRMFCLSPC 478
TP DN +SF**NHVHWNQC+ FCL PC
Sbjct: 119 TPSDNPVSF**NHVHWNQCKTFCLLPC 39
Score = 252 bits (545), Expect(3) = 1e-070
Identities = 117/177 (66%), Positives = 126/177 (71%)
Frame = +2 / +1
Query: 443 KWKRSSN*NCYKHGDKQNILH*FQWT*FHQKDKRLSIGVED*SAPAYCWKLLSC*SWHLC 622
KWKR+ N*N Y HG+KQN+LH*FQWT*FHQKD LS+GVED*S P CWKLLS *SWHLC
Sbjct: 4 KWKRTFN*NHY*HGNKQNVLH*FQWT*FHQKDTGLSLGVED*SKPTCCWKLLSY*SWHLC 183
Query: 623 GGWQQRAVCTGRQVNWRFQYKGWTNRANATQEATP**WERRRRGAK*NSLSR*AM*RTHY 802
G WQQR V T RQV WRF++KGWTN A AT EAT **W K* SL *+M*RT Y
Sbjct: 184 GRWQQRVVYTCRQVCWRFEHKGWTNIAYATHEATQ**WSWCCGSTK*KSLFG*SM*RTTY 363
Query: 803 TRKILCQNRPTRRRSSVASDIWPGNILPSPSCFHRAGWRQLGEFTCCKILCDGFNLQ 973
RKIL N PTR R VAS I +LPS SC RA WRQL FTC LC+G NLQ
Sbjct: 364 *RKILS*N*PTR*RG*VASYIGSRTVLPSSSCVLRARWRQLA*FTCFISLCNGTNLQ 534
Score = 238 bits (515), Expect = 5e-062
Identities = 119/201 (59%), Positives = 133/201 (66%)
Frame = +1 / +3
Query: 481 W*QTKHSTLIPVDVISSKG*EIIDRSGRLKCTSXXXXXXXXXXXASMWRMAAKSCLYW*T 660
W QTK TLIPVDVISSKG II RSGRLK T+ A MW+MA KSCLY T
Sbjct: 42 WQQTKRFTLIPVDVISSKGYGIIARSGRLK*TNLLLETIILLILAFMWKMATKSCLYLQT 221
Query: 661 GQLEVPV*RMDK*S*CYTGGYSMMMGKASPRR*MKQSVSISNVKDSLYKENTMSK*THKE 840
G LEV *RMDK S CYT GYSMMM + *MK+SV + NVKD L KENT+ K THK
Sbjct: 222 GLLEVRA*RMDKYSLCYT*GYSMMMVVVLRKH*MKKSVRMINVKDYLLKENTILKLTHKV 401
Query: 841 KELGGVGHLARKYTPLSFLLSQSRMEATGRIHMLQNSLRWIQLTACLIMLQCLPFRSLKM 1020
LGGV H + TPL FL SQS+MEATG IHM SL+W QLT L +++CL R+LK
Sbjct: 402 TGLGGVLHWVKNCTPLFFLRSQSKMEATGLIHMFHFSLQWNQLTVFL*LVRCLRLRNLKT 581
Query: 1021 EVFSFGLLIYMRPGRTRIFRL 1083
EVFSFG L Y R G RI +L
Sbjct: 582 EVFSFGWLTYTRLGNIRISQL 644
Score = 135 bits (290), Expect(2) = 1e-052
Identities = 65/108 (60%), Positives = 70/108 (64%)
Frame = -3 / -3
Query: 1090 LAKDERSLSSPASYR*ANXXXXXXXXXXXXXSIATLSGKL*VESIAENFATCEFAQLPPS 911
+A+ ERSL ASYR*AN S ++G+L*V SIAE TCE QLPPS
Sbjct: 651 IARVERSLCFQASYR*ANRRRTLPSSSSSSVSNVPITGRL*VGSIAERNETCELGQLPPS 472
Query: 910 CSVKARRRGEYISWPNVRRHRAPSPCGSILT*YFPCIMSPSHCLSRQT 767
CS ARRRGEY S PNV RH APSPCGSIL *YFP I SPSH S QT
Sbjct: 471 CSENARRRGEYSS*PNVGRHLAPSPCGSILR*YFPSISSPSH*SSEQT 328
Score = 95.9 bits (203), Expect(2) = 1e-052
Identities = 44/53 (83%), Positives = 46/53 (86%)
Frame = -3 / -3
Query: 601 G**FPAIGWCTSIFHSDR*SLILLMKSRPLESV*NVLFVTMFVTISVATSFPF 443
G**FPA GW TSIFHS+R*S ILLMKSRPLESV*NVLFV M V ISV +SFPF
Sbjct: 162 G**FPATGWFTSIFHSER*SRILLMKSRPLESV*NVLFVAMLVMISVESSFPF 4
Score = 27.6 bits (54), Expect(3) = 1e-070
Identities = 14/21 (66%), Positives = 14/21 (66%)
Frame = +2 / +1
Query: 995 AYPSGA*RWKCSPSVCSSI*G 1057
AY G *R KCSPSV S I G
Sbjct: 556 AYA*GT*RRKCSPSVGSPIRG 618
Score = 26.7 bits (52), Expect(3) = 1e-070
Identities = 10/14 (71%), Positives = 12/14 (85%)
Frame = +3 / +3
Query: 1092 IDLKRVFPEKKIGK 1133
+ LKRVFPE K+GK
Sbjct: 654 LTLKRVFPE*KVGK 695
Score = 25.8 bits (50), Expect(2) = 1e-081
Identities = 9/14 (64%), Positives = 12/14 (85%)
Frame = -3 / -2
Query: 1138 MTLPIFFSGNTLLR 1097
++LP F+SG TLLR
Sbjct: 700 LSLPTFYSGKTLLR 659
>gb|BQ469437.1|BQ469437 HM04L06r HM Hordeum vulgare subsp. vulgare cDNA clone HM04L06
5-PRIME, mRNA sequence
Length = 618
Score = 207 bits (447), Expect(2) = e-102
Identities = 87/124 (70%), Positives = 100/124 (80%)
Frame = +2 / +2
Query: 20 KKSAHISSKSTQYSPQGSESSNLQVGQGNLKLQYNEAGKLSLYSDSKTMVQANFEQKYKY 199
+K + S + + QGS+S+NLQVGQG LKLQY+ AG LS YSDSKT V+ANFEQKYKY
Sbjct: 8 RKRSASISSKSTLNSQGSKSTNLQVGQGYLKLQYDAAGALSHYSDSKTRVEANFEQKYKY 187
Query: 200 YIGQDGNGSDPQASGAYIFRPNGTVPISTDGQVSLTVLRGPILDEVHQQINSWIYQITRR 379
Y+GQDG G+DPQASGAYIFRP VPI TDGQV +LRGPILDEVHQQINSWIYQITR
Sbjct: 188 YVGQDGYGNDPQASGAYIFRPKDVVPIKTDGQVPPMILRGPILDEVHQQINSWIYQITRV 367
Query: 380 LQGE 391
+G+
Sbjct: 368 YKGK 379
Score = 186 bits (401), Expect(2) = e-102
Identities = 72/84 (85%), Positives = 81/84 (96%)
Frame = +3 / +2
Query: 378 VYKEKDFLETEFIVGPIPVDDGNGKEVATEIVTNMVTNKTFYTDSSGRDFIKRIRDYRSE 557
VYK KD++ETEFIVGPIPVDD NGKE++TEI+T+M TNKTFYTDSSGRDFIKRIRDYRSE
Sbjct: 365 VYKGKDYVETEFIVGPIPVDDENGKELSTEIITSMATNKTFYTDSSGRDFIKRIRDYRSE 544
Query: 558 WKIEVHQPIAGNYYPVNLGIYVED 629
WKIEV+QP+AGNYYP+NLGIYVED
Sbjct: 545 WKIEVNQPVAGNYYPINLGIYVED 616
Score = 180 bits (388), Expect(3) = 2e-084
Identities = 81/108 (75%), Positives = 85/108 (78%)
Frame = -1 / -1
Query: 387 PCKRLVI**IHEFICWCTSSNMGPRRTVRETCPSVLIGTVPLGRNMYAPEA*GSLPFPSC 208
P LVI* IHE ICWCTSSNMGPRR + T PSVLIGT LGRNMYAPEA*GS P+PSC
Sbjct: 375 PL*TLVI*YIHELICWCTSSNMGPRRIMGGT*PSVLIGTTSLGRNMYAPEA*GSFPYPSC 196
Query: 207 PI*YLYFCSKFA*TIVLLSEYRDNFPASLY*SFKLP*PTCRLLLSLP* 64
P *YLYFCSKFA T VLLSE DN PA+ Y*SFK P PTCR +L LP*
Sbjct: 195 PT*YLYFCSKFASTRVLLSE*WDNAPAASY*SFKYPCPTCRFVLLLP* 52
Score = 156 bits (336), Expect(3) = 8e-079
Identities = 63/84 (75%), Positives = 71/84 (84%)
Frame = +2 / +1
Query: 380 LQGEGLFGN*IYSWAHTSR*WKWKRSSN*NCYKHGDKQNILH*FQWT*FHQKDKRLSIGV 559
LQG+GL GN*++SWA+TSR KWKR+ N*N Y HG+KQN+LH*FQWT*FHQKD LS+GV
Sbjct: 367 LQGKGLCGN*VHSWANTSRRRKWKRTFN*NHY*HGNKQNVLH*FQWT*FHQKDTGLSLGV 546
Query: 560 ED*SAPAYCWKLLSC*SWHLCGGW 631
ED*S P CWKLLS *SWHLCG W
Sbjct: 547 ED*SKPTCCWKLLSY*SWHLCGRW 618
Score = 149 bits (319), Expect(3) = 8e-079
Identities = 67/102 (65%), Positives = 76/102 (74%)
Frame = +3 / +3
Query: 63 LKEVKVVIYKLVKAT*NFNIMKLENYPCTPIVRLWFKQTLSRNTSIILDRMEMEVILRLL 242
L+EVKV KL K *NFN+M+LE+YP TPI RL KQ SR+TSI D+M+ E+ILRLL
Sbjct: 51 LREVKVRTCKLGKGI*NFNMMQLEHYPTTPIARLGLKQISSRSTSIT*DKMDTEMILRLL 230
Query: 243 EHTYSALMAQFQSVLMGRFLSQFCVGPYWMKCTSR*IHGFIR 368
E TY AL F S LM RFL FCVGPYWMKCTSR IHG+IR
Sbjct: 231 ERTYFALRTSFLSKLMVRFLP*FCVGPYWMKCTSRLIHGYIR 356
Score = 148 bits (317), Expect(3) = 2e-084
Identities = 62/85 (72%), Positives = 71/85 (83%)
Frame = -2 / -3
Query: 629 ILHIDAKINRIIVSSNRLVHFNLPLRSIISYPFDEITSTGISVECFVCHHVCNNFSCYFF 450
I HI+AKINRIIVSSNRLV+FNLPLR+II YPFDEITSTGISV+ FVC H N+FS FF
Sbjct: 616 IFHINAKINRIIVSSNRLVYFNLPLRAIIPYPFDEITSTGISVKRFVCCHASNDFS*KFF 437
Query: 449 SISIIYWYGPNYKFSFQKVLLLVNA 375
SI ++YWY PNY+ SF VL LVN+
Sbjct: 436 SIFVVYWYWPNYELSFHIVLSLVNS 362
Score = 147 bits (315), Expect(3) = 2e-068
Identities = 67/103 (65%), Positives = 77/103 (74%)
Frame = -2 / -2
Query: 371 *SDKSMNLSAGALHPIWAHAEL*EKPAHQY*LELCH*GGICMLQKPEDHFHFHPVQYNTC 192
*SD SMN SAGALHPIWAHAE E+P HQ+* E *G IC LQKPEDHF H V NTC
Sbjct: 359 *SDISMN*SAGALHPIWAHAESWEEPDHQF**ERRP*GEICTLQKPEDHFRIHLVLRNTC 180
Query: 191 ISAQSLLEP*SYYRSTGIIFQLHYIEVSSCLDQLVDYYFHFLE 63
SA++LL+P S YRS+GI+ QLH+IEVS+ L QL YF+F E
Sbjct: 179 TSARNLLQPESCYRSSGIMLQLHHIEVSNTLAQLAGSYFYFPE 51
Score = 141 bits (302), Expect(3) = 3e-048
Identities = 68/103 (66%), Positives = 75/103 (72%)
Frame = +1 / +1
Query: 64 SRK*K**STSWSRQLETSI**SWKIIPVLR**DYGSSKL*AEIQVLYWTGWKWK*SSGFW 243
S K*K SW+R ETSI* SW IIP+LR* D G SK AE+QVL T W K*SSGFW
Sbjct: 52 SGK*KYEPASWARVFETSI*CSWSIIPLLR*QDSG*SKFRAEVQVLRRTRWIRK*SSGFW 231
Query: 244 SIHIPP*WHSSNQY*WAGFSHSSAWAHIG*SAPADKFMDLSDY 372
S+HI P* S Q *W+G SH SAWAHIG*SAPAD FMD+SD+
Sbjct: 232 SVHISP*GRRSYQN*WSGSSHDSAWAHIG*SAPAD*FMDISDH 360
Score = 127 bits (273), Expect(3) = 2e-068
Identities = 60/75 (80%), Positives = 64/75 (85%)
Frame = -3 / -1
Query: 601 G**FPAIGWCTSIFHSDR*SLILLMKSRPLESV*NVLFVTMFVTISVATSFPFPSSTGMG 422
G**FPA GW TSIFHS+R*S ILLMKSRPLESV*NVLFV M V ISV +SFPF SSTG+G
Sbjct: 588 G**FPATGWFTSIFHSER*SRILLMKSRPLESV*NVLFVAMLVMISVESSFPFSSSTGIG 409
Query: 421 PTINSVSKKSFSL*T 377
PT+NSVS SF L*T
Sbjct: 408 PTMNSVST*SFPL*T 364
Score = 100 bits (212), Expect(4) = 3e-049
Identities = 39/51 (76%), Positives = 42/51 (82%)
Frame = -1 / -2
Query: 630 HPPHRCQD*QDNSFQQ*AGALQSSTPIDNLLSF**NHVHWNQCRMFCLSPC 478
H PH+CQD* DNSFQQ G LQSSTP DN +SF**NHVHWNQC+ FCL PC
Sbjct: 617 HLPHKCQD**DNSFQQQVGLLQSSTPSDNPVSF**NHVHWNQCKTFCLLPC 465
Score = 64.8 bits (135), Expect(4) = 3e-049
Identities = 31/47 (65%), Positives = 34/47 (72%)
Frame = -3 / -3
Query: 388 SL*TPGNLINP*IYLLVHFIQYGPTQNCERNLPISTDWNCAIRAEYV 248
SL +LI P*I LLVHFIQYGPTQN RNL IS D N +RA+YV
Sbjct: 376 SLVNSSDLIYP*INLLVHFIQYGPTQNHGRNLTISFDRNDVLRAKYV 236
Score = 59.3 bits (123), Expect(3) = 3e-048
Identities = 29/50 (58%), Positives = 31/50 (62%)
Frame = +1 / +3
Query: 481 W*QTKHSTLIPVDVISSKG*EIIDRSGRLKCTSXXXXXXXXXXXASMWRM 630
W QTK TLIPVDVISSKG II RSGRLK T+ A MW+M
Sbjct: 468 WQQTKRFTLIPVDVISSKGYGIIARSGRLK*TNLLLETIILLILAFMWKM 617
Score = 57.0 bits (118), Expect(4) = 3e-049
Identities = 26/40 (65%), Positives = 30/40 (75%)
Frame = -3 / -3
Query: 181 KVCLNHSLTIGVQG*FSSFIILKFQVALTNL*ITTFTSLR 62
++C N SL IGV G* SS IILKFQ+ L NL + TFTSLR
Sbjct: 169 EICFNPSLAIGVVG*CSSCIILKFQIPLPNLQVRTFTSLR 50
Score = 31.3 bits (62), Expect(3) = 3e-048
Identities = 12/17 (70%), Positives = 13/17 (76%)
Frame = +1 / +3
Query: 379 FTRRRTFWKLNL*LGPY 429
FTR RT WKL+ *LG Y
Sbjct: 366 FTRERTMWKLSS*LGQY 416
Score = 31.3 bits (62), Expect(4) = 3e-049
Identities = 13/17 (76%), Positives = 13/17 (76%)
Frame = -1 / -2
Query: 429 VWAQL*IQFPKSPSPCK 379
V AQL* QFP SP PCK
Sbjct: 416 VLAQL*TQFPHSPFPCK 366
Score = 28.6 bits (56), Expect(3) = 8e-079
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = +1 / +1
Query: 10 RKLEEISTYFFEVHSVLTSR 69
R EEIS Y FE+HS L+ +
Sbjct: 1 RHEEEISIYLFEIHSKLSGK 60
Score = 24.9 bits (48), Expect(3) = 2e-084
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = -2 / -2
Query: 77 FHFLEVSTEWTSKKYVLISSS 15
+ + S EW SK+ +LISSS
Sbjct: 68 YFYFPESLEWISKR*MLISSS 6
Score = 24.4 bits (47), Expect(3) = 2e-068
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = -1 / -1
Query: 60 EY*VDFEEICADFF 19
E+ VDFEEI AD F
Sbjct: 51 EFRVDFEEIDADLF 10
>gb|BQ659809.1|BQ659809 HF01M14w HF Hordeum vulgare subsp. vulgare cDNA clone HF01M14
3-PRIME, mRNA sequence
Length = 656
Score = 363 bits (786), Expect = 2e-099
Identities = 146/177 (82%), Positives = 162/177 (91%)
Frame = +3 / -3
Query: 789 EGLIIQGKYYVKIDPQGEGARWRRTFGQEIYSPLLLAFTEQDGGNWANSHVAKFSAMDST 968
EGL+I+GKYY+KIDPQG+GARWRRTFGQE+YSPLLLAF EQDGGNWANSHV+ FSAMD T
Sbjct: 654 EGLVIEGKYYLKIDPQGDGARWRRTFGQELYSPLLLAFAEQDGGNWANSHVSSFSAMDPT 475
Query: 969 YSLPDNVAMLTLQELEDGSVLLRFAHLYEAGEDKDLSSLASIDLKRVFPEKKIGKVIETS 1148
YSLP+NVA+LTL+ELEDGSVLLR AHLYEAGE KDLS+LAS+DLKRVFP+KK+GK+IETS
Sbjct: 474 YSLPENVALLTLEELEDGSVLLRLAHLYEAGEHKDLSALASVDLKRVFPDKKVGKIIETS 295
Query: 1149 LSANQERAAMEKKRLKWKAQGSAADEKVVRGGPVDPSKLVVELGPMEIRTFIVSFDH 1319
LSANQERAAMEKKRLKWK G E V RGGPVDPSKLVVEL PMEIRTF++SF H
Sbjct: 294 LSANQERAAMEKKRLKWKVAGPPPKENVARGGPVDPSKLVVELAPMEIRTFVISFTH 124
Score = 274 bits (592), Expect = 1e-072
Identities = 124/175 (70%), Positives = 129/175 (73%)
Frame = -3 / +2
Query: 1312 KLTMNVRISIGPSSTTSFEGSTGPPRTTFSSAAEPCAFHFKRFFSMAARSWFADKLVSMT 1133
KL NVRIS+G SSTTS EGSTGPPR TFS P FHF FFSMAARSWFAD+LVS+
Sbjct: 131 KLMTNVRISMGASSTTSLEGSTGPPRATFSFGGGPATFHFSLFFSMAARSWFADRLVSII 310
Query: 1132 LPIFFSGNTLLRSILAKDERSLSSPASYR*ANXXXXXXXXXXXXXSIATLSGKL*VESIA 953
LP F SGNTLLRS LA+ ERSL SPASYR*AN S AT SG+L*V SIA
Sbjct: 311 LPTFLSGNTLLRSTLARAERSLCSPASYR*ANRRRTLPSSSSSSVSNATFSGRL*VGSIA 490
Query: 952 ENFATCEFAQLPPSCSVKARRRGEYISWPNVRRHRAPSPCGSILT*YFPCIMSPS 788
E TCEFAQLPPSCS ARRRGEY S PNVRRH APSPCGSIL *YFP I SPS
Sbjct: 491 EKDETCEFAQLPPSCSANARRRGEYSS*PNVRRHLAPSPCGSILR*YFPSITSPS 655
Score = 208 bits (449), Expect = 6e-053
Identities = 102/175 (58%), Positives = 113/175 (64%)
Frame = +2 / -2
Query: 788 *RTHYTRKILCQNRPTRRRSSVASDIWPGNILPSPSCFHRAGWRQLGEFTCCKILCDGFN 967
*RT Y RKIL QN PTR R VAS IW G +LPS SC RAGWRQLGEFTC LC+G N
Sbjct: 655 *RTSY*RKILSQN*PTR*RG*VASYIWSGTVLPSSSCVRRAGWRQLGEFTCFIFLCNGSN 476
Query: 968 LQLA**CCNAYPSGA*RWKCSPSVCSSI*GRGGQGSFVFSEYRPQESVPGEEDWQGHRDK 1147
LQ +* C AY G *RWKCSPSV S I G GS E RPQE +P + Q + +K
Sbjct: 475 LQSS*KCRVAYA*GT*RWKCSPSVGSPIRGWRA*GSLSSGECRPQEGIPR*KGRQDN*NK 296
Query: 1148 LIGKPRTRSHGEEAFEMEGARFCGGREGGSGWPCGSFEARR*AGANGDPHIHRQL 1312
I +PR RSHGEE EMEG R R+ GS G +ARR*AGA+GDPHI QL
Sbjct: 295 PISEPRARSHGEEEAEMEGGRASTKRKCGSWRTRGPLQARR*AGAHGDPHIRHQL 131
Score = 202 bits (436), Expect = 4e-051
Identities = 98/171 (57%), Positives = 114/171 (66%)
Frame = -1 / +3
Query: 1299 MCGSPLAPAQRRASKDPQGHPEPPSRPPQNLAPSISNASSPWLRVLGLPISLSR*PCQSS 1120
MCGSP APAQRRA + P+ EP + L PSIS +SSPWLR LG I L + C+
Sbjct: 144 MCGSPWAPAQRRAWRGPRVLHEPHFLLVEALPPSISASSSPWLRALGSLIGLFQLSCRPF 323
Query: 1119 SPGTLS*GRYSLKTKDPCPPRPHIDEQTEGEHFHLQAPEG*ALQHYQASCKLNPSQRILQ 940
G S*GR+S + +DP +P I E TEGEHFHLQ P+ *A +H+Q CKL+P QR ++
Sbjct: 324 YLGIPS*GRHSPELRDPYALQPRIGEPTEGEHFHLQVPQA*ATRHFQEDCKLDPLQRKMK 503
Query: 939 HVNSPSCLHPAL*KQEGEGSIFPGQMSDATELLLLVGLF*HSIFLV**VLH 787
HVNSPSCLHPAL QE EGS P QM DAT LVG F* SIFL * VLH
Sbjct: 504 HVNSPSCLHPALRTQEEEGSTVPDQMYDAT*PRHLVGQF*DSIFLQ*LVLH 656
Score = 151 bits (324), Expect(2) = 2e-052
Identities = 71/108 (65%), Positives = 82/108 (75%)
Frame = -2 / +1
Query: 1112 EHSLEVDTR*RRKILVLPGLI*MSKPKENTSIFKLLKGKHCNIIRQAVS*IHRREFCNM* 933
E+ LEVDTR +IL+L L+*+S+PKENTSIFK LK K +I R+ VS IH RE NM*
Sbjct: 331 EYPLEVDTRQS*EILMLSSLV*VSQPKENTSIFKFLKRKQRDIFRKTVSWIHCRER*NM* 510
Query: 932 IRPVASILLCESKKERGVYFLAKCPTPPSSFSLWVYFDIVFSLYNESF 789
IRPVASILLCE KK+RGV FL KC TPPS +LWV F+IVFS N SF
Sbjct: 511 IRPVASILLCERKKKRGVQFLTKCTTPPSPVTLWVNFEIVFSFNN*SF 654
Score = 135 bits (290), Expect(2) = 3e-048
Identities = 65/99 (65%), Positives = 70/99 (70%)
Frame = +1 / -1
Query: 787 VKDSLYKENTMSK*THKEKELGGVGHLARKYTPLSFLLSQSRMEATGRIHMLQNSLRWIQ 966
VKD L KENT+SK THK LGGV HL R TPL FL SQSRMEATGRIHM SL+WIQ
Sbjct: 656 VKD*LLKENTISKLTHKVTGLGGVVHLVRNCTPLFFLRSQSRMEATGRIHMFHLSLQWIQ 477
Query: 967 LTACLIMLQCLPFRSLKMEVFSFGLLIYMRPGRTRIFRL 1083
LT L M +CL R+LKMEVFSFG L Y R RI +L
Sbjct: 476 LTVFLKMSRCLRLRNLKMEVFSFGWLTYTRLESIRISQL 360
Score = 77.6 bits (163), Expect(2) = 3e-048
Identities = 36/68 (52%), Positives = 42/68 (61%)
Frame = +1 / -1
Query: 1144 QAYRQTKNAQPWRRSV*NGRRKVLRRTRRWFGVALWILRSSSLSWGQWRSAHSSSALITS 1323
QAY++TK+AQPWRR *NGR + L + + W W SSSLSW WRSAHSSSA T
Sbjct: 299 QAYQRTKSAQPWRRRG*NGRWQGLHQKKMWLVEDPWTPPSSSLSWRPWRSAHSSSASPTD 120
Query: 1324 VTSNSSNR 1347
S R
Sbjct: 119 SPHVRSER 96
Score = 76.2 bits (160), Expect(2) = 2e-052
Identities = 31/52 (59%), Positives = 37/52 (71%)
Frame = -2 / +1
Query: 1313 KADDECADLHWPQLNDELRRIHRATPNHLLVRRRTLRLPFQTLLLHGCAFLV 1158
+ADDECADLH QLNDEL +H ++ +H+ R LPFQ LLLHGCA LV
Sbjct: 130 EADDECADLHGRQLNDELGGVHGSSTSHIFFWWRPCHLPFQPLLLHGCALLV 285
>gb|CA017868.1|CA017868 HV06J10r HV Hordeum vulgare subsp. vulgare cDNA clone HV06J10
5-PRIME, mRNA sequence
Length = 500
Score = 360 bits (780), Expect = 1e-098
Identities = 141/164 (85%), Positives = 159/164 (96%)
Frame = +3 / +1
Query: 402 ETEFIVGPIPVDDGNGKEVATEIVTNMVTNKTFYTDSSGRDFIKRIRDYRSEWKIEVHQP 581
E EFIVGPIPVDD NGKE++TEI+T+M TNKTFYTDSSGRDFIKRIRDYRSEWKIEV+QP
Sbjct: 7 EAEFIVGPIPVDDENGKELSTEIITSMATNKTFYTDSSGRDFIKRIRDYRSEWKIEVNQP 186
Query: 582 IAGNYYPVNLGIYVEDGSKELSVLVDRSIGGSSIKDGQIELMLHRRLLHDDGKGVAEALN 761
+AGNYYP+NLGIYVEDG+KELS+LVDRS+GGSSIKDGQIELMLHRRLL+DDG+GVAEAL+
Sbjct: 187 VAGNYYPINLGIYVEDGNKELSILVDRSVGGSSIKDGQIELMLHRRLLNDDGRGVAEALD 366
Query: 762 ETVCLDKQCEGLIIQGKYYVKIDPQGEGARWRRTFGQEIYSPLL 893
E VCLD QCEGL+I+GKYY+KIDPQG+GARWRRTFGQE+YSPLL
Sbjct: 367 EKVCLDDQCEGLVIEGKYYLKIDPQGDGARWRRTFGQELYSPLL 498
Score = 267 bits (577), Expect = 1e-070
Identities = 116/163 (71%), Positives = 132/163 (80%)
Frame = -2 / -3
Query: 893 KERGVYFLAKCPTPPSSFSLWVYFDIVFSLYNESFTLLIETDCFI*RLGDAFPIIME*PP 714
K+RGV FL KC TPPS +LWV F+IVFS N SFTL+I+TD FI* + II+E*PP
Sbjct: 498 KKRGVQFLTKCTTPPSPVTLWVNFEIVFSFNN*SFTLIIQTDFFI*CFRNTTTIIIE*PP 319
Query: 713 V*H*LYLSILYTGTSN*PVYQYRQLFAAILHIDAKINRIIVSSNRLVHFNLPLRSIISYP 534
V*H*LYLSILY TSN PVY+YRQLF AI HI+AKINRIIVSSNRLV+FNLPLR+II YP
Sbjct: 318 V*H*LYLSILYARTSNRPVYKYRQLFVAIFHINAKINRIIVSSNRLVYFNLPLRAIIPYP 139
Query: 533 FDEITSTGISVECFVCHHVCNNFSCYFFSISIIYWYGPNYKFS 405
FDEITSTGISV+ FVC H N+FS FFSI ++YWY PNY+ S
Sbjct: 138 FDEITSTGISVKRFVCCHASNDFS*KFFSIFVVYWYWPNYELS 10
Score = 253 bits (548), Expect = 1e-066
Identities = 113/163 (69%), Positives = 125/163 (76%)
Frame = +2 / +3
Query: 407 *IYSWAHTSR*WKWKRSSN*NCYKHGDKQNILH*FQWT*FHQKDKRLSIGVED*SAPAYC 586
*++SWA+TSR KWKR+ N*N Y HG+KQN+LH*FQWT*FHQKD LS+GVED*S P C
Sbjct: 12 *VHSWANTSRRRKWKRTFN*NHY*HGNKQNVLH*FQWT*FHQKDTGLSLGVED*SKPTCC 191
Query: 587 WKLLSC*SWHLCGGWQQRAVCTGRQVNWRFQYKGWTNRANATQEATP**WERRRRGAK*N 766
WKLLS *SWHLCG WQQR V T RQV WRF++KGWTNRANATQEAT **W +*
Sbjct: 192 WKLLSY*SWHLCGRWQQRVVYTCRQVCWRFEHKGWTNRANATQEATQ**WSWCCGSTR*K 371
Query: 767 SLSR*AM*RTHYTRKILCQNRPTRRRSSVASDIWPGNILPSPS 895
SL *+M*RT Y RKIL QN PTR R VAS IW G +LPS S
Sbjct: 372 SLFG*SM*RTSY*RKILSQN*PTR*RG*VASYIWSGTVLPSSS 500
Score = 212 bits (457), Expect = 5e-054
Identities = 96/139 (69%), Positives = 102/139 (73%)
Frame = -1 / -2
Query: 894 EGEGSIFPGQMSDATELLLLVGLF*HSIFLV**VLHIAYRDRLFHLAPRRRLSHHHGVAS 715
E EGS P QM DAT LVG F* SIFL * VLHI + +RLFHL + HHH VAS
Sbjct: 499 EEEGSTVPDQMYDAT*PRHLVGQF*DSIFLQ*LVLHIDHPNRLFHLVLPQHHDHHH*VAS 320
Query: 714 CVALALFVHPLYWNLQLTCLPVQTALCCHPPHRCQD*QDNSFQQ*AGALQSSTPIDNLLS 535
CVALALFVHPL NLQ TCL V T LCCH PH+CQD* DNSFQQ G LQSSTP DN +S
Sbjct: 319 CVALALFVHPLCSNLQQTCLQV*TTLCCHLPHKCQD**DNSFQQQVGLLQSSTPSDNPVS 140
Query: 534 F**NHVHWNQCRMFCLSPC 478
F**NHVHWNQC+ FCL PC
Sbjct: 139 F**NHVHWNQCKTFCLLPC 83
Score = 178 bits (383), Expect = 8e-044
Identities = 89/138 (64%), Positives = 95/138 (68%)
Frame = +1 / +2
Query: 481 W*QTKHSTLIPVDVISSKG*EIIDRSGRLKCTSXXXXXXXXXXXASMWRMAAKSCLYW*T 660
W QTK TLIPVDVISSKG II RSGRLK T+ A MW+MA KSCLY *T
Sbjct: 86 WQQTKRFTLIPVDVISSKGYGIIARSGRLK*TNLLLETIILLILAFMWKMATKSCLYL*T 265
Query: 661 GQLEVPV*RMDK*S*CYTGGYSMMMGKASPRR*MKQSVSISNVKDSLYKENTMSK*THKE 840
G LEV *RMDK*S*CYTGGYSMMM + *MK+SV + NVKD L KENT+SK THK
Sbjct: 266 GLLEVRA*RMDK*S*CYTGGYSMMMVVVLRKH*MKKSVWMINVKD*LLKENTISKLTHKV 445
Query: 841 KELGGVGHLARKYTPLSF 894
LGGV HL R TPL F
Sbjct: 446 TGLGGVVHLVRNCTPLFF 499
Score = 119 bits (255), Expect(2) = 3e-043
Identities = 54/67 (80%), Positives = 58/67 (86%)
Frame = -3 / -1
Query: 601 G**FPAIGWCTSIFHSDR*SLILLMKSRPLESV*NVLFVTMFVTISVATSFPFPSSTGMG 422
G**FPA GW TSIFHS+R*S ILLMKSRPLESV*NVLFV M V ISV +SFPF SSTG+G
Sbjct: 206 G**FPATGWFTSIFHSER*SRILLMKSRPLESV*NVLFVAMLVMISVESSFPFSSSTGIG 27
Query: 421 PTINSVS 401
PT+NS S
Sbjct: 26 PTMNSAS 6
Score = 79.9 bits (168), Expect(2) = 3e-043
Identities = 34/43 (79%), Positives = 35/43 (81%)
Frame = -3 / -1
Query: 895 RRRGEYISWPNVRRHRAPSPCGSILT*YFPCIMSPSHCLSRQT 767
RRRGEY S PNVRRH APSPCGSIL *YFP I SPSH S+QT
Sbjct: 500 RRRGEYSS*PNVRRHLAPSPCGSILR*YFPSITSPSH*SSKQT 372
>gb|AV920015.1|AV920015 AV920015 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags8p06 3', mRNA sequence
Length = 561
Score = 336 bits (727), Expect = 3e-091
Identities = 136/164 (82%), Positives = 149/164 (90%)
Frame = +3 / -1
Query: 828 DPQGEGARWRRTFGQEIYSPLLLAFTEQDGGNWANSHVAKFSAMDSTYSLPDNVAMLTLQ 1007
DPQG+GARWRRTFGQE+YSPLLLAF EQDGGNWANSHV+ FSAMD TYSLP+NVA+LTL+
Sbjct: 561 DPQGDGARWRRTFGQELYSPLLLAFAEQDGGNWANSHVSSFSAMDPTYSLPENVALLTLE 382
Query: 1008 ELEDGSVLLRFAHLYEAGEDKDLSSLASIDLKRVFPEKKIGKVIETSLSANQERAAMEKK 1187
ELEDGSVLLR AHLYEAGE KDLS+LAS+DLKRVFP+KK+GK+IETSLSANQERAAMEKK
Sbjct: 381 ELEDGSVLLRLAHLYEAGEHKDLSALASVDLKRVFPDKKVGKIIETSLSANQERAAMEKK 202
Query: 1188 RLKWKAQGSAADEKVVRGGPVDPSKLVVELGPMEIRTFIVSFDH 1319
RLKWK G E V RGGPVDPSKLVVEL PMEIRTF++SF H
Sbjct: 201 RLKWKVAGPPPKENVARGGPVDPSKLVVELAPMEIRTFVISFTH 70
Score = 251 bits (543), Expect = 7e-066
Identities = 113/161 (70%), Positives = 118/161 (73%)
Frame = -3 / +2
Query: 1312 KLTMNVRISIGPSSTTSFEGSTGPPRTTFSSAAEPCAFHFKRFFSMAARSWFADKLVSMT 1133
KL NVRIS+G SSTTS EGSTGPPR TFS P FHF FFSMAARSWFAD+LVS+
Sbjct: 77 KLMTNVRISMGASSTTSLEGSTGPPRATFSFGGGPATFHFSLFFSMAARSWFADRLVSII 256
Query: 1132 LPIFFSGNTLLRSILAKDERSLSSPASYR*ANXXXXXXXXXXXXXSIATLSGKL*VESIA 953
LP F SGNTLLRS LA+ ERSL SPASYR*AN S AT SG+L*V SIA
Sbjct: 257 LPTFLSGNTLLRSTLARAERSLCSPASYR*ANRRRTLPSSSSSSVSNATFSGRL*VGSIA 436
Query: 952 ENFATCEFAQLPPSCSVKARRRGEYISWPNVRRHRAPSPCG 830
E TCEFAQLPPSCS ARRRGEY S PNVRRH APSPCG
Sbjct: 437 EKDETCEFAQLPPSCSANARRRGEYSS*PNVRRHLAPSPCG 559
Score = 187 bits (402), Expect = 2e-046
Identities = 90/161 (55%), Positives = 101/161 (62%)
Frame = +2 / -3
Query: 830 PTRRRSSVASDIWPGNILPSPSCFHRAGWRQLGEFTCCKILCDGFNLQLA**CCNAYPSG 1009
PTR R VAS IW G +LPS SC RAGWRQLGEFTC LC+G NLQ +* C AY G
Sbjct: 559 PTR*RG*VASYIWSGTVLPSSSCVRRAGWRQLGEFTCFIFLCNGSNLQSS*KCRVAYA*G 380
Query: 1010 A*RWKCSPSVCSSI*GRGGQGSFVFSEYRPQESVPGEEDWQGHRDKLIGKPRTRSHGEEA 1189
*RWKCSPSV S I G GS E RPQE +P + Q + +K I +PR R HGEE
Sbjct: 379 T*RWKCSPSVGSPIRGWRA*GSLSSGECRPQEGIPR*KGRQDN*NKPISEPRARRHGEEE 200
Query: 1190 FEMEGARFCGGREGGSGWPCGSFEARR*AGANGDPHIHRQL 1312
EMEG R R+ G G +ARR*AGA+GDPHI QL
Sbjct: 199 AEMEGGRASTKRKCGPWRTRGPLQARR*AGAHGDPHIRHQL 77
Score = 181 bits (390), Expect = 9e-045
Identities = 86/157 (54%), Positives = 102/157 (64%)
Frame = -1 / +3
Query: 1299 MCGSPLAPAQRRASKDPQGHPEPPSRPPQNLAPSISNASSPWLRVLGLPISLSR*PCQSS 1120
MCGSP APAQRRA + P+ P + L PSIS +SSPW R LG I L + C+
Sbjct: 90 MCGSPWAPAQRRAWRGPRVLHGPHFLLVEALPPSISASSSPWRRALGSLIGLFQLSCRPF 269
Query: 1119 SPGTLS*GRYSLKTKDPCPPRPHIDEQTEGEHFHLQAPEG*ALQHYQASCKLNPSQRILQ 940
G S*GR+S + +DP +P I E TEGEHFHLQ P+ *A +H+Q CKL+P QR ++
Sbjct: 270 YLGIPS*GRHSPELRDPYALQPRIGEPTEGEHFHLQVPQA*ATRHFQEDCKLDPLQRKMK 449
Query: 939 HVNSPSCLHPAL*KQEGEGSIFPGQMSDATELLLLVG 829
HVNSPSCLHPAL QE EGS P QM DAT LVG
Sbjct: 450 HVNSPSCLHPALRTQEEEGSTVPDQMYDAT*PRHLVG 560
Score = 135 bits (290), Expect(2) = 5e-046
Identities = 63/95 (66%), Positives = 73/95 (76%)
Frame = -2 / +1
Query: 1112 EHSLEVDTR*RRKILVLPGLI*MSKPKENTSIFKLLKGKHCNIIRQAVS*IHRREFCNM* 933
E+ LEVDTR +IL+L L+*+S+PKENTSIFK LK K +I R+ VS IH RE NM*
Sbjct: 277 EYPLEVDTRQS*EILMLSSLV*VSQPKENTSIFKFLKRKQRDIFRKTVSWIHCRER*NM* 456
Query: 932 IRPVASILLCESKKERGVYFLAKCPTPPSSFSLWV 828
IRPVASILLCE KK+RGV FL KC TPPS +LWV
Sbjct: 457 IRPVASILLCERKKKRGVQFLTKCTTPPSPVTLWV 561
Score = 116 bits (249), Expect(2) = 3e-041
Identities = 55/85 (64%), Positives = 59/85 (69%)
Frame = +1 / -2
Query: 829 THKEKELGGVGHLARKYTPLSFLLSQSRMEATGRIHMLQNSLRWIQLTACLIMLQCLPFR 1008
THK LGGV HL R TPL FL SQSRMEATGRIHM SL+WIQLT L M +CL R
Sbjct: 560 THKVTGLGGVVHLVRNCTPLFFLRSQSRMEATGRIHMFHLSLQWIQLTVFLKMSRCLRLR 381
Query: 1009 SLKMEVFSFGLLIYMRPGRTRIFRL 1083
+LKMEVFSFG L Y R RI +L
Sbjct: 380 NLKMEVFSFGWLTYTRLESIRISQL 306
Score = 73.0 bits (153), Expect(2) = 3e-041
Identities = 35/68 (51%), Positives = 41/68 (60%)
Frame = +1 / -2
Query: 1144 QAYRQTKNAQPWRRSV*NGRRKVLRRTRRWFGVALWILRSSSLSWGQWRSAHSSSALITS 1323
QAY++TK+A PWRR *NGR + L + + W W SSSLSW WRSAHSSSA T
Sbjct: 245 QAYQRTKSAPPWRRRG*NGRWQGLHQKKMWPVEDPWTPPSSSLSWRPWRSAHSSSASPTD 66
Query: 1324 VTSNSSNR 1347
S R
Sbjct: 65 SPHVRSER 42
Score = 70.3 bits (147), Expect(2) = 5e-046
Identities = 30/52 (57%), Positives = 35/52 (67%)
Frame = -2 / +1
Query: 1313 KADDECADLHWPQLNDELRRIHRATPNHLLVRRRTLRLPFQTLLLHGCAFLV 1158
+ADDECADLH QLNDEL +H ++ H+ R LPFQ LLLHG A LV
Sbjct: 76 EADDECADLHGRQLNDELGGVHGSSTGHIFFWWRPCHLPFQPLLLHGGALLV 231
>gb|AV917216.1|AV917216 AV917216 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags8p06 5', mRNA sequence
Length = 518
Score = 319 bits (690), Expect(2) = 4e-089
Identities = 127/150 (84%), Positives = 141/150 (94%)
Frame = +3 / +2
Query: 762 ETVCLDKQCEGLIIQGKYYVKIDPQGEGARWRRTFGQEIYSPLLLAFTEQDGGNWANSHV 941
E VCLD QCEGL+I+GKYY+KIDPQG+GARWRRTFGQE+YSPLLLAF EQDGGNWANSHV
Sbjct: 62 EKVCLDDQCEGLVIEGKYYLKIDPQGDGARWRRTFGQELYSPLLLAFAEQDGGNWANSHV 241
Query: 942 AKFSAMDSTYSLPDNVAMLTLQELEDGSVLLRFAHLYEAGEDKDLSSLASIDLKRVFPEK 1121
+ FSAMD TYSLP+NVA+LTL+ELEDGSVLLR AHLYEAGE KDLS+LAS+DLKRVFP+K
Sbjct: 242 SSFSAMDPTYSLPENVALLTLEELEDGSVLLRLAHLYEAGEHKDLSALASVDLKRVFPDK 421
Query: 1122 KIGKVIETSLSANQERAAMEKKRLKWKAQG 1211
K+GK+IETSLSANQERAAMEKKRLKWK G
Sbjct: 422 KVGKIIETSLSANQERAAMEKKRLKWKVAG 511
Score = 230 bits (496), Expect = 2e-059
Identities = 104/148 (70%), Positives = 109/148 (73%)
Frame = -3 / -3
Query: 1210 PCAFHFKRFFSMAARSWFADKLVSMTLPIFFSGNTLLRSILAKDERSLSSPASYR*ANXX 1031
P FHF FFSMAARSWFAD+LVS+ LP F SGNTLLRS LA+ ERSL SPASYR*AN
Sbjct: 510 PATFHFSLFFSMAARSWFADRLVSIILPTFLSGNTLLRSTLARAERSLCSPASYR*ANRR 331
Query: 1030 XXXXXXXXXXXSIATLSGKL*VESIAENFATCEFAQLPPSCSVKARRRGEYISWPNVRRH 851
S AT SG+L*V SIAE TCEFAQLPPSCS ARRRGEY S PNVRRH
Sbjct: 330 RTLPSSSSSSVSNATFSGRL*VGSIAEKDETCEFAQLPPSCSANARRRGEYSS*PNVRRH 151
Query: 850 RAPSPCGSILT*YFPCIMSPSHCLSRQT 767
APSPCGSIL *YFP I SPSH S+QT
Sbjct: 150 LAPSPCGSILR*YFPSITSPSH*SSKQT 67
Score = 183 bits (394), Expect = 2e-045
Identities = 87/150 (58%), Positives = 102/150 (68%)
Frame = -1 / -1
Query: 1209 LAPSISNASSPWLRVLGLPISLSR*PCQSSSPGTLS*GRYSLKTKDPCPPRPHIDEQTEG 1030
L PSIS +SSPW R LG I L + C+ G S*GR+S + +DP +P I E TEG
Sbjct: 509 LPPSISASSSPWRRALGSLIGLFQLSCRPFYLGIPS*GRHSPELRDPYALQPRIGEPTEG 330
Query: 1029 EHFHLQAPEG*ALQHYQASCKLNPSQRILQHVNSPSCLHPAL*KQEGEGSIFPGQMSDAT 850
EHFHLQ P+ *A +H+Q CKL+P QR ++HVNSPSCLHPAL QE EGS P QM DAT
Sbjct: 329 EHFHLQVPQA*ATRHFQEDCKLDPLQRKMKHVNSPSCLHPALRTQEEEGSTVPDQMYDAT 150
Query: 849 ELLLLVGLF*HSIFLV**VLHIAYRDRLFH 760
LVG F* SIFL * VLHI + +RLFH
Sbjct: 149 *PRHLVGQF*DSIFLQ*LVLHIDHPNRLFH 60
Score = 177 bits (382), Expect = 1e-043
Identities = 88/150 (58%), Positives = 97/150 (64%)
Frame = +2 / +1
Query: 761 *NSLSR*AM*RTHYTRKILCQNRPTRRRSSVASDIWPGNILPSPSCFHRAGWRQLGEFTC 940
* SL *+M*RT Y RKIL QN PTR R VAS IW G +LPS SC RAGWRQLGEFTC
Sbjct: 61 *KSLFG*SM*RTSY*RKILSQN*PTR*RG*VASYIWSGTVLPSSSCVRRAGWRQLGEFTC 240
Query: 941 CKILCDGFNLQLA**CCNAYPSGA*RWKCSPSVCSSI*GRGGQGSFVFSEYRPQESVPGE 1120
LC+G NLQ +* C AY G *RWKCSPSV S I G GS E RPQE +P
Sbjct: 241 FIFLCNGSNLQSS*KCRVAYA*GT*RWKCSPSVGSPIRGWRA*GSLSSGECRPQEGIPR* 420
Query: 1121 EDWQGHRDKLIGKPRTRSHGEEAFEMEGAR 1210
+ Q + +K I +PR R HGEE EMEG R
Sbjct: 421 KGRQDN*NKPISEPRARRHGEEEAEMEGGR 510
Score = 166 bits (356), Expect(2) = 5e-043
Identities = 77/117 (65%), Positives = 90/117 (76%)
Frame = -2 / -2
Query: 1112 EHSLEVDTR*RRKILVLPGLI*MSKPKENTSIFKLLKGKHCNIIRQAVS*IHRREFCNM* 933
E+ LEVDTR +IL+L L+*+S+PKENTSIFK LK K +I R+ VS IH RE NM*
Sbjct: 412 EYPLEVDTRQS*EILMLSSLV*VSQPKENTSIFKFLKRKQRDIFRKTVSWIHCRER*NM* 233
Query: 932 IRPVASILLCESKKERGVYFLAKCPTPPSSFSLWVYFDIVFSLYNESFTLLIETDCF 762
IRPVASILLCE KK+RGV FL KC TPPS +LWV F+IVFS N SFTL+I+TD F
Sbjct: 232 IRPVASILLCERKKKRGVQFLTKCTTPPSPVTLWVNFEIVFSFNN*SFTLIIQTDFF 62
Score = 145 bits (311), Expect(2) = 6e-039
Identities = 70/108 (64%), Positives = 77/108 (71%)
Frame = +1 / +3
Query: 760 MKQSVSISNVKDSLYKENTMSK*THKEKELGGVGHLARKYTPLSFLLSQSRMEATGRIHM 939
MK+SV + NVKD L KENT+SK THK LGGV HL R TPL FL SQSRMEATGRIHM
Sbjct: 60 MKKSVWMINVKD*LLKENTISKLTHKVTGLGGVVHLVRNCTPLFFLRSQSRMEATGRIHM 239
Query: 940 LQNSLRWIQLTACLIMLQCLPFRSLKMEVFSFGLLIYMRPGRTRIFRL 1083
SL+WIQLT L M +CL R+LKMEVFSFG L Y R RI +L
Sbjct: 240 FHLSLQWIQLTVFLKMSRCLRLRNLKMEVFSFGWLTYTRLESIRISQL 383
Score = 36.8 bits (74), Expect(2) = 6e-039
Identities = 14/20 (70%), Positives = 17/20 (85%)
Frame = +1 / +3
Query: 1144 QAYRQTKNAQPWRRSV*NGR 1203
QAY++TK+A PWRR *NGR
Sbjct: 444 QAYQRTKSAPPWRRRG*NGR 503
Score = 30.9 bits (61), Expect(2) = 4e-089
Identities = 12/14 (85%), Positives = 14/14 (100%)
Frame = +3 / +1
Query: 717 RLLHDDGKGVAEAL 758
RLL+DDG+GVAEAL
Sbjct: 16 RLLNDDGRGVAEAL 57
Score = 29.9 bits (59), Expect(2) = 5e-043
Identities = 13/19 (68%), Positives = 13/19 (68%)
Frame = -2 / -2
Query: 1214 RTLRLPFQTLLLHGCAFLV 1158
R LPFQ LLLHG A LV
Sbjct: 514 RPCHLPFQPLLLHGGALLV 458
>gb|BQ661666.1|BQ661666 HM04L06u HM Hordeum vulgare subsp. vulgare cDNA clone HM04L06
3-PRIME, mRNA sequence
Length = 579
Score = 278 bits (602), Expect = 5e-074
Identities = 114/140 (81%), Positives = 125/140 (89%)
Frame = +3 / -3
Query: 900 FTEQDGGNWANSHVAKFSAMDSTYSLPDNVAMLTLQELEDGSVLLRFAHLYEAGEDKDLS 1079
F EQDGGNWANSHV+ FSAMD TYSLP+NVA+LTL+ELEDGSVLLR AHLYEAGE KDLS
Sbjct: 577 FAEQDGGNWANSHVSSFSAMDPTYSLPENVALLTLEELEDGSVLLRLAHLYEAGEHKDLS 398
Query: 1080 SLASIDLKRVFPEKKIGKVIETSLSANQERAAMEKKRLKWKAQGSAADEKVVRGGPVDPS 1259
+LAS+DLKRVFP+KK+GK+IETSLSANQERAAMEKKRLKWK G E V RGGPVDPS
Sbjct: 397 ALASVDLKRVFPDKKVGKIIETSLSANQERAAMEKKRLKWKVAGPPPKENVARGGPVDPS 218
Query: 1260 KLVVELGPMEIRTFIVSFDH 1319
KLVVEL PMEIRTF++SF H
Sbjct: 217 KLVVELAPMEIRTFVISFTH 158
Score = 203 bits (438), Expect = 2e-051
Identities = 93/136 (68%), Positives = 98/136 (72%)
Frame = -3 / +3
Query: 1312 KLTMNVRISIGPSSTTSFEGSTGPPRTTFSSAAEPCAFHFKRFFSMAARSWFADKLVSMT 1133
KL NVRIS+G SSTTS EGSTGPPR TFS P FHF FFSMAARSWFAD+LVS+
Sbjct: 165 KLMTNVRISMGASSTTSLEGSTGPPRATFSFGGGPATFHFSLFFSMAARSWFADRLVSII 344
Query: 1132 LPIFFSGNTLLRSILAKDERSLSSPASYR*ANXXXXXXXXXXXXXSIATLSGKL*VESIA 953
LP F SGNTLLRS LA+ ERSL SPASYR*AN S AT SG+L*V SIA
Sbjct: 345 LPTFLSGNTLLRSTLARAERSLCSPASYR*ANRRRTLPSSSSSSVSNATFSGRL*VGSIA 524
Query: 952 ENFATCEFAQLPPSCS 905
E TCEFAQLPPSCS
Sbjct: 525 EKDETCEFAQLPPSCS 572
Score = 166 bits (356), Expect = 4e-040
Identities = 74/132 (56%), Positives = 90/132 (68%)
Frame = -1 / +1
Query: 1299 MCGSPLAPAQRRASKDPQGHPEPPSRPPQNLAPSISNASSPWLRVLGLPISLSR*PCQSS 1120
MCGSP APAQRRA + P+ EP + L PSIS +SSPWLR LG I L + C+
Sbjct: 178 MCGSPWAPAQRRAWRGPRVLHEPHFLLVEALPPSISASSSPWLRALGSLIGLFQLSCRPF 357
Query: 1119 SPGTLS*GRYSLKTKDPCPPRPHIDEQTEGEHFHLQAPEG*ALQHYQASCKLNPSQRILQ 940
G S*GR+S + +DP +P I E TEGEHFHLQ P+ *A +H+Q CKL+P QR ++
Sbjct: 358 YLGIPS*GRHSPELRDPYALQPRIGEPTEGEHFHLQVPQA*ATRHFQEDCKLDPLQRKMK 537
Query: 939 HVNSPSCLHPAL 904
HVNSPSCLHPAL
Sbjct: 538 HVNSPSCLHPAL 573
Score = 158 bits (340), Expect = 7e-038
Identities = 77/136 (56%), Positives = 87/136 (63%)
Frame = +2 / -2
Query: 905 RAGWRQLGEFTCCKILCDGFNLQLA**CCNAYPSGA*RWKCSPSVCSSI*GRGGQGSFVF 1084
RAGWRQLGEFTC LC+G NLQ +* C AY G *RWKCSPSV S I G GS
Sbjct: 572 RAGWRQLGEFTCFIFLCNGSNLQSS*KCRVAYA*GT*RWKCSPSVGSPIRGWRA*GSLSS 393
Query: 1085 SEYRPQESVPGEEDWQGHRDKLIGKPRTRSHGEEAFEMEGARFCGGREGGSGWPCGSFEA 1264
E RPQE +P + Q + +K I +PR RSHGEE EMEG R R+ GS G +A
Sbjct: 392 GECRPQEGIPR*KGRQDN*NKPISEPRARSHGEEEAEMEGGRASTKRKCGSWRTRGPLQA 213
Query: 1265 RR*AGANGDPHIHRQL 1312
RR*AGA+GDPHI QL
Sbjct: 212 RR*AGAHGDPHIRHQL 165
Score = 95.5 bits (202), Expect(2) = 8e-036
Identities = 47/71 (66%), Positives = 55/71 (77%)
Frame = -2 / +2
Query: 1112 EHSLEVDTR*RRKILVLPGLI*MSKPKENTSIFKLLKGKHCNIIRQAVS*IHRREFCNM* 933
E+ LEVDTR +IL+L L+*+S+PKENTSIFK LK K +I R+ VS IH RE NM*
Sbjct: 365 EYPLEVDTRQS*EILMLSSLV*VSQPKENTSIFKFLKRKQRDIFRKTVSWIHCRER*NM* 544
Query: 932 IRPVASILLCE 900
IRPVASILLCE
Sbjct: 545 IRPVASILLCE 577
Score = 86.3 bits (182), Expect(2) = 2e-033
Identities = 40/61 (65%), Positives = 44/61 (72%)
Frame = +1 / -1
Query: 901 SQSRMEATGRIHMLQNSLRWIQLTACLIMLQCLPFRSLKMEVFSFGLLIYMRPGRTRIFR 1080
SQSRMEATGRIHM SL+WIQLT L M +CL R+LKMEVFSFG L Y R RI +
Sbjct: 576 SQSRMEATGRIHMFHLSLQWIQLTVFLKMSRCLRLRNLKMEVFSFGWLTYTRLESIRISQ 397
Query: 1081 L 1083
L
Sbjct: 396 L 394
Score = 77.6 bits (163), Expect(2) = 2e-033
Identities = 36/68 (52%), Positives = 42/68 (61%)
Frame = +1 / -1
Query: 1144 QAYRQTKNAQPWRRSV*NGRRKVLRRTRRWFGVALWILRSSSLSWGQWRSAHSSSALITS 1323
QAY++TK+AQPWRR *NGR + L + + W W SSSLSW WRSAHSSSA T
Sbjct: 333 QAYQRTKSAQPWRRRG*NGRWQGLHQKKMWLVEDPWTPPSSSLSWRPWRSAHSSSASPTD 154
Query: 1324 VTSNSSNR 1347
S R
Sbjct: 153 SPHVRSER 130
Score = 76.2 bits (160), Expect(2) = 8e-036
Identities = 31/52 (59%), Positives = 37/52 (71%)
Frame = -2 / +2
Query: 1313 KADDECADLHWPQLNDELRRIHRATPNHLLVRRRTLRLPFQTLLLHGCAFLV 1158
+ADDECADLH QLNDEL +H ++ +H+ R LPFQ LLLHGCA LV
Sbjct: 164 EADDECADLHGRQLNDELGGVHGSSTSHIFFWWRPCHLPFQPLLLHGCALLV 319
>gb|CV054336.1|CV054336 BNEL109d2 Barley EST endosperm library Hordeum vulgare subsp. vulgare
cDNA clone BNEL109d2 5' similar to Zea mays PCO061004
mRNA sequence
Length = 595
Score = 149 bits (320), Expect = 4e-035
Identities = 76/155 (49%), Positives = 92/155 (59%)
Frame = +3 / +1
Query: 669 GGSSIKDGQIELMLHRRLLHDDGKGVAEALNETVCLDKQCEGLIIQGKYYVKIDPQGEGA 848
G +K M +++ H G A E LD Q I KY++KI PQ + A
Sbjct: 130 GRFELKGWPNRAMAPQKVTHCYWPGGAGTFKEKSWLDYQWGRPSIGRKYHLKIGPQWDRA 309
Query: 849 RWRRTFGQEIYSPLLLAFTEQDGGNWANSHVAKFSAMDSTYSLPDNVAMLTLQELEDGSV 1028
W TFGQE PL AF EQDGGN ANSHV+ FSAMD TY LP+NVA+LTL+ELE+ +V
Sbjct: 310 WWPPTFGQEPDFPLFFAFAEQDGGNCANSHVSSFSAMDPTYRLPENVALLTLEELENRNV 489
Query: 1029 LLRFAHLYEAGEDKDLSSLASIDLKRVFPEKKIGK 1133
LLR AHLYEAGE DLS L + L KK+ +
Sbjct: 490 LLRVAHLYEAGEHMDLS*LWRV*L*EGITYKKVAR 594
Score = 143 bits (307), Expect = 2e-033
Identities = 62/111 (55%), Positives = 79/111 (71%)
Frame = +3 / +2
Query: 540 RDYRSEWKIEVHQPIAGNYYPVNLGIYVEDGSKELSVLVDRSIGGSSIKDGQIELMLHRR 719
RDYRSEWKIEV+QP+AGNYYP+NLGIYVEDG+KELS+LV++++ GSS+KDGQIEL LHRR
Sbjct: 2 RDYRSEWKIEVNQPVAGNYYPINLGIYVEDGNKELSILVNKAVEGSSLKDGQIELWLHRR 181
Query: 720 LLHDDGKGVAEALNETVCLDKQCEGLIIQGKYYVKIDPQGEGARWRRTFGQ 872
LL G+GV E L + E L++ +K+ G G R G+
Sbjct: 182 LLIVIGRGVPEHLKKKAGWIINGEDLVLDENTILKLAHNGTGLGGLRHLGK 334
Score = 122 bits (260), Expect(2) = 2e-048
Identities = 65/126 (51%), Positives = 78/126 (61%)
Frame = -2 / -2
Query: 1103 LEVDTR*RRKILVLPGLI*MSKPKENTSIFKLLKGKHCNIIRQAVS*IHRREFCNM*IRP 924
LE+ + R+I +L L+*+S KEN SIFK LK K +I R++VS IHRRE NM*IR
Sbjct: 564 LELHSPELREIHMLSSLV*VSHAKENISIFKFLKCKQGHIFRKSVSWIHRRER*NM*IRT 385
Query: 923 VASILLCESKKERGVYFLAKCPTPPSSFSLWVYFDIVFSLYNESFTLLIETDCFI*RLGD 744
VASILLCE KK+R V FL KC PPS LW F +VFS S L+I+ F G
Sbjct: 384 VASILLCERKKKREVRFLPKCRRPPSPVPLWANFKMVFSSNTRSSPLIIQPAFFFKCSGT 205
Query: 743 AFPIIM 726
PI M
Sbjct: 204 PRPITM 187
Score = 109 bits (233), Expect(2) = 3e-046
Identities = 53/110 (48%), Positives = 68/110 (61%)
Frame = +1 / +2
Query: 727 MMMGKASPRR*MKQSVSISNVKDSLYKENTMSK*THKEKELGGVGHLARKYTPLSFLLSQ 906
+++G+ P K++ I N +D + ENT+ K H LGG+ HL + T L FL SQ
Sbjct: 188 IVIGRGVPEHLKKKAGWIINGEDLVLDENTILKLAHNGTGLGGLRHLGKNRTSLFFLRSQ 367
Query: 907 SRMEATGRIHMLQNSLRWIQLTACLIMLQCLPFRSLKMEVFSFGLLIYMR 1056
S+MEAT RIHM SLRWIQLT L M CL R+LK+E+FSF L Y R
Sbjct: 368 SKMEATVRIHMFHLSLRWIQLTDFLKMWPCLHLRNLKIEMFSFAWLTYTR 517
Score = 106 bits (227), Expect(2) = 1e-043
Identities = 55/103 (53%), Positives = 61/103 (59%)
Frame = -3 / -3
Query: 1078 ERSLSSPASYR*ANXXXXXXXXXXXXXSIATLSGKL*VESIAENFATCEFAQLPPSCSVK 899
ERS+ SPASYR*A S AT SG L*V SIAE TCEFAQLPPSCS
Sbjct: 539 ERSICSPASYR*ATRRRTFLFSSSSSVSKATFSGSL*VGSIAEKDETCEFAQLPPSCSAN 360
Query: 898 ARRRGEYISWPNVRRHRAPSPCGSILT*YFPCIMSPSHCLSRQ 770
A++RG+ S PNV H+A S CG IL YF I+ H S Q
Sbjct: 359 AKKRGKSGSCPNVGGHQARSHCGPILRWYFRPILGLPH**SNQ 231
Score = 104 bits (222), Expect(2) = 6e-027
Identities = 51/98 (52%), Positives = 64/98 (65%)
Frame = -1 / -1
Query: 1077 KDPCPPRPHIDEQTEGEHFHLQAPEG*ALQHYQASCKLNPSQRILQHVNSPSCLHPAL*K 898
+DP +P I E EGEHF+ Q P+ *A H+Q CKL+PSQR ++HVNS SCLH AL
Sbjct: 538 RDPYALQPRIGEPREGEHFYFQVPQV*ARPHFQEVCKLDPSQRKMKHVNSHSCLHLALRT 359
Query: 897 QEGEGSIFPGQMSDATELLLLVGLF*HSIFLV**VLHI 784
Q+ EGS QMS+AT+ +VG F* IF+ *V I
Sbjct: 358 QKKEGSPVLAQMSEATKPGPIVGQF*DGIFVQY*VFPI 245
Score = 97.3 bits (206), Expect(2) = 3e-046
Identities = 41/64 (64%), Positives = 45/64 (70%)
Frame = +2 / +1
Query: 545 LSIGVED*SAPAYCWKLLSC*SWHLCGGWQQRAVCTGRQVNWRFQYKGWTNRANATQEAT 724
LS+GVED*S P CWKLLS *SWHLCG WQQR V T +Q RF+ KGW NRA A Q+ T
Sbjct: 7 LSLGVED*SKPTCCWKLLSY*SWHLCGRWQQRVVYTCKQGCGRFELKGWPNRAMAPQKVT 186
Query: 725 P**W 736
W
Sbjct: 187 HCYW 198
Score = 92.7 bits (196), Expect(2) = 1e-030
Identities = 51/100 (51%), Positives = 56/100 (56%)
Frame = +2 / +3
Query: 782 AM*RTHYTRKILCQNRPTRRRSSVASDIWPGNILPSPSCFHRAGWRQLGEFTCCKILCDG 961
+M +T Y KI N PT VASDIW LPS C RA WRQL EFTC LCDG
Sbjct: 243 SMGKT*YWTKIPS*NWPTMGPGLVASDIWARTGLPSFFCVRRARWRQLCEFTCFIFLCDG 422
Query: 962 FNLQLA**CCNAYPSGA*RWKCSPSVCSSI*GRGGQGSFV 1081
NLQ +* C AY G *+ KCSPS S I G GS +
Sbjct: 423 SNLQTS*KCGLAYT*GT*K*KCSPSRGSPIRGWRAYGSLL 542
Score = 92.3 bits (195), Expect(2) = 2e-048
Identities = 40/60 (66%), Positives = 42/60 (70%)
Frame = -1 / -3
Query: 723 VASCVALALFVHPLYWNLQLTCLPVQTALCCHPPHRCQD*QDNSFQQ*AGALQSSTPIDN 544
V C A+ALF HPL NL CL V T LCCH PH+CQD* DNSFQQ G LQSSTP DN
Sbjct: 185 VTFCGAIALFGHPLSSNLPQPCLQV*TTLCCHLPHKCQD**DNSFQQQVGLLQSSTPSDN 6
Score = 90.9 bits (192), Expect(2) = 1e-043
Identities = 44/61 (72%), Positives = 50/61 (81%)
Frame = -2 / -1
Query: 725 E*PPV*H*LYLSILYTGTSN*PVYQYRQLFAAILHIDAKINRIIVSSNRLVHFNLPLRSI 546
E*P V *LYL+IL T + VY+YRQLF AI HI+AKINRIIVSSNRLV+FNLPLR+I
Sbjct: 187 E*PSVEP*LYLAIL*ARTFHSLVYKYRQLFVAIFHINAKINRIIVSSNRLVYFNLPLRAI 8
Query: 545 I 543
I
Sbjct: 7 I 5
Score = 61.6 bits (128), Expect(2) = 1e-030
Identities = 36/80 (45%), Positives = 44/80 (55%)
Frame = +1 / +3
Query: 544 IIDRSGRLKCTSXXXXXXXXXXXASMWRMAAKSCLYW*TGQLEVPV*RMDK*S*CYTGGY 723
II RSGRLK T+ A MW+MA KSCLY *T +V *RM K*S T GY
Sbjct: 6 IIARSGRLK*TNLLLETIILLILAFMWKMATKSCLYL*TRLWKVRA*RMAK*SYGSTEGY 185
Query: 724 SMMMGKASPRR*MKQSVSIS 783
S+++ * K+ V +S
Sbjct: 186 SLLLAGGCRNI*RKKLVGLS 245
Score = 40.5 bits (82), Expect(2) = 6e-027
Identities = 17/20 (85%), Positives = 18/20 (90%)
Frame = -3 / -2
Query: 601 G**FPAIGWCTSIFHSDR*S 542
G**FPA GW TSIFHS+R*S
Sbjct: 63 G**FPATGWFTSIFHSER*S 4
>gb|AV833861.1|AV833861 AV833861 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags18d19, mRNA sequence
Length = 551
Score = 116 bits (248), Expect(2) = 2e-044
Identities = 42/65 (64%), Positives = 53/65 (81%)
Frame = +3 / +2
Query: 378 VYKEKDFLETEFIVGPIPVDDGNGKEVATEIVTNMVTNKTFYTDSSGRDFIKRIRDYRSE 557
+YK+KD E E+ +GPIPVDDG GKEV T + NMVTN TFYTDS+GRDF+KR+RDYR +
Sbjct: 353 LYKDKDHAEVEYTIGPIPVDDGIGKEVITRLTANMVTNHTFYTDSNGRDFLKRVRDYRED 532
Query: 558 WKIEV 572
W ++V
Sbjct: 533 WDLQV 547
Score = 84.0 bits (177), Expect(2) = 2e-044
Identities = 31/59 (52%), Positives = 41/59 (69%)
Frame = +2 / +2
Query: 200 YIGQDGNGSDPQASGAYIFRPNGTVPISTDGQVSLTVLRGPILDEVHQQINSWIYQITR 376
+ G D QASGAYIFRP+G+ P + + L V+RGP++DEVHQQ + WIYQ+TR
Sbjct: 176 WYGSRTGDGDSQASGAYIFRPDGSTPTAVSRSIPLKVIRGPLVDEVHQQFSPWIYQVTR 352
>gb|AV834608.1|AV834608 AV834608 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp. vulgare
cDNA clone bags18d19, mRNA sequence
Length = 609
Score = 153 bits (329), Expect(2) = 3e-043
Identities = 60/116 (51%), Positives = 85/116 (73%)
Frame = +3 / -1
Query: 837 GEGARWRRTFGQEIYSPLLLAFTEQDGGNWANSHVAKFSAMDSTYSLPDNVAMLTLQELE 1016
G GA WRRT GQ++YSP LL F +D +W + V K S MD+ YSLPDNVA++TLQ L+
Sbjct: 609 GHGAHWRRTQGQKVYSPFLLGFAHEDESSWKSYSVVKESMMDANYSLPDNVAIITLQNLD 430
Query: 1017 DGSVLLRFAHLYEAGEDKDLSSLASIDLKRVFPEKKIGKVIETSLSANQERAAMEK 1184
DG+ LLR HL++A ED S +A ++LK++F ++ I ++ ET+LSANQ+++AM K
Sbjct: 429 DGTTLLRLGHLFQAAEDPQYSVMAKVELKKLFGKRTIKELTETNLSANQKKSAMRK 262
Score = 43.2 bits (88), Expect(2) = 3e-043
Identities = 18/25 (72%), Positives = 20/25 (80%)
Frame = +3 / -1
Query: 1233 VRGGPVDPSKLVVELGPMEIRTFIV 1307
V G PV+ LVVELGPMEIRTF+V
Sbjct: 216 VTGSPVNDKVLVVELGPMEIRTFLV 142
>gb|BQ664507.1|BQ664507 HV03K06u HV Hordeum vulgare subsp. vulgare cDNA clone HV03K06
3-PRIME, mRNA sequence
Length = 409
Score = 175 bits (377), Expect = 5e-043
Identities = 72/90 (80%), Positives = 79/90 (87%)
Frame = +3 / -2
Query: 1050 YEAGEDKDLSSLASIDLKRVFPEKKIGKVIETSLSANQERAAMEKKRLKWKAQGSAADEK 1229
YEAGE KDLS+LAS+DLKRVFP+KK+GK+IETSLSANQERAAMEKKRLKWK G E
Sbjct: 408 YEAGEHKDLSALASVDLKRVFPDKKVGKIIETSLSANQERAAMEKKRLKWKVAGPPPKEN 229
Query: 1230 VVRGGPVDPSKLVVELGPMEIRTFIVSFDH 1319
V RGGPVDPSKLVVEL PMEIRTF++SF H
Sbjct: 228 VARGGPVDPSKLVVELAPMEIRTFVISFTH 139
Score = 150 bits (322), Expect = 2e-035
Identities = 65/88 (73%), Positives = 69/88 (78%)
Frame = -3 / +2
Query: 1312 KLTMNVRISIGPSSTTSFEGSTGPPRTTFSSAAEPCAFHFKRFFSMAARSWFADKLVSMT 1133
KL NVRIS+G SSTTS EGSTGPPR TFS P FHF FFSMAARSWFAD+LVS+
Sbjct: 146 KLMTNVRISMGASSTTSLEGSTGPPRATFSFGGGPATFHFSLFFSMAARSWFADRLVSII 325
Query: 1132 LPIFFSGNTLLRSILAKDERSLSSPASY 1049
LP F SGNTLLRS LA+ ERSL SPASY
Sbjct: 326 LPTFLSGNTLLRSTLARAERSLCSPASY 409
>gb|BI951455.1|BI951455 HVSMEl0025P22f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0025P22f, mRNA sequence
Length = 523
Score = 155 bits (332), Expect = 9e-037
Identities = 60/69 (86%), Positives = 67/69 (97%)
Frame = +3 / +3
Query: 402 ETEFIVGPIPVDDGNGKEVATEIVTNMVTNKTFYTDSSGRDFIKRIRDYRSEWKIEVHQP 581
ETEFIVGPIPVDD NGKE++TEI+T+M TNKTFYTDSSGRDFIKRIRDYRSEWKIEV+QP
Sbjct: 3 ETEFIVGPIPVDDENGKELSTEIITSMATNKTFYTDSSGRDFIKRIRDYRSEWKIEVNQP 182
Query: 582 IAGNYYPVN 608
+AGNYYPV+
Sbjct: 183 VAGNYYPVS 209
Score = 126 bits (270), Expect = 3e-028
Identities = 51/68 (75%), Positives = 58/68 (85%)
Frame = +2 / +2
Query: 401 GN*IYSWAHTSR*WKWKRSSN*NCYKHGDKQNILH*FQWT*FHQKDKRLSIGVED*SAPA 580
GN*++SWA+TSR KWKR+ N*N Y HG+KQN+LH*FQWT*FHQKD LS+GVED*S P
Sbjct: 2 GN*VHSWANTSRR*KWKRTVN*NHY*HGNKQNVLH*FQWT*FHQKDTGLSLGVED*SKPT 181
Query: 581 YCWKLLSC 604
CWKLLSC
Sbjct: 182 CCWKLLSC 205
Score = 125 bits (267), Expect = 8e-028
Identities = 51/70 (72%), Positives = 59/70 (84%)
Frame = -2 / -3
Query: 611 KINRIIVSSNRLVHFNLPLRSIISYPFDEITSTGISVECFVCHHVCNNFSCYFFSISIIY 432
+ +RIIVSSNRLV+FNLPLR+II YPFDEITSTGISV+ FVC H N+FS FFSI I+Y
Sbjct: 212 RAHRIIVSSNRLVYFNLPLRAIIPYPFDEITSTGISVKRFVCCHASNDFS*QFFSIFIVY 33
Query: 431 WYGPNYKFSF 402
WY PNY+ SF
Sbjct: 32 WYWPNYELSF 3
Score = 121 bits (258), Expect = 1e-026
Identities = 55/67 (82%), Positives = 59/67 (88%)
Frame = -3 / -1
Query: 601 G**FPAIGWCTSIFHSDR*SLILLMKSRPLESV*NVLFVTMFVTISVATSFPFPSSTGMG 422
G**FPA GW TSIFHS+R*S ILLMKSRPLESV*NVLFV M V ISV +SFPF SSTG+G
Sbjct: 202 G**FPATGWFTSIFHSER*SRILLMKSRPLESV*NVLFVAMLVMISVDSSFPFSSSTGIG 23
Query: 421 PTINSVS 401
PT+NSVS
Sbjct: 22 PTMNSVS 2
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 800,768,693
Number of Sequences: 312970
Number of extensions: 14300505
Number of successful extensions: 141031
Number of sequences better than 1.0e-020: 14
length of database: 58,378,179
effective HSP length: 54
effective length of database: 41,477,799
effective search space used: 20199688113
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)