TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= QAS4f06.yg.3.1
         (768 letters)

Database: Hordeum_nucl_with_EST.fasta 
           312,970 sequences; 175,134,539 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|BF256838.2|BF256838  HVSMEf0011C11f Hordeum vulgare seedl...   108   4e-035

>gb|BF256838.2|BF256838 HVSMEf0011C11f Hordeum vulgare seedling root EST library HVcDNA0007
           (Etiolated and unstressed) Hordeum vulgare subsp.
           vulgare cDNA clone HVSMEf0011C11f, mRNA sequence
          Length = 582

 Score =  108 bits (231), Expect(4) = 4e-035
 Identities = 42/87 (48%), Positives = 50/87 (57%)
 Frame = -3 / -2

                                                                       
Query: 526 HLESGNGRDCCWNRSWNGWQGSGNRGHWWQGNRDCRNSWYGGWNHRVWERWDCASSCWWQ 347
           HLE  NGRDCCW    NG     + GHWW G+R  RN  +GGW+ R W+ W+ A  C   
Sbjct: 443 HLELRNGRDCCWYHDRNGRNSGWD*GHWWHGSRQSRNGRHGGWDRRNWK*WNRARGCRCH 264

                                      
Query: 346 G*LWDRNGRDWWDCHLGDLRNGRHWRQ 266
           G L DRN      C L + RNGR+WRQ
Sbjct: 263 GDLRDRNEWYRRHCQLRNCRNGRNWRQ 183




 Score =  100 bits (213), Expect(3) = 3e-032
 Identities = 39/96 (40%), Positives = 58/96 (60%)
 Frame = +1 / +2

                                                                       
Query: 268 ASNAFHSGGPQDDNPTNPVHSYPKASPATNSCWRNPISPKPGGSNHRTSYFCSPGYPATN 447
           ASN+FHS   + DN  + +HSYP+    T+S W   I+   G  NH   +     Y ATN
Sbjct: 185 ASNSFHSCSSEADNAADTIHSYPEGRHGTDSPWHGSITSNSGDPNHHADHSYFACYHATN 364

                                               
Query: 448 ALDSHYLAIHSNYDSNNNPYHSRTPGASIHRPIPTN 555
           AL+ ++ ++HS++D+NNNPYHS  P A+    +PT+
Sbjct: 365 ALNPNHYSVHSDHDTNNNPYHS*VPDATYSIHVPTS 472




 Score = 45.1 bits (92), Expect(3) = 3e-032
 Identities = 18/38 (47%), Positives = 23/38 (60%)
 Frame = +1 / +2

                                                 
Query: 145 STSLGHSSCARGHACSAVHSSGSNQTASSADHTSGSNH 258
           S+  GH SC RGH   A ++ GSN TAS A +   SN+
Sbjct: 68  SSPFGHGSCTRGHTSPAFYT*GSNDTASDAYNCGSSNY 181




 Score = 39.1 bits (79), Expect(4) = 4e-035
 Identities = 20/45 (44%), Positives = 24/45 (53%)
 Frame = -3 / -2

                                                        
Query: 265 RQRGWNRWYGRHWRQFGWNRWNGRLSRRGLWRSCCVQEMSSCKAA 131
           RQ  WN    RH RQ  W+    RL   GLW SC VQ+ +S + A
Sbjct: 188 RQCSWNCRNCRHRRQCHWSLRCRRLGWCGLWCSCRVQKATSRRGA 54




 Score = 32.2 bits (64), Expect(4) = 4e-035
 Identities = 12/21 (57%), Positives = 15/21 (71%)
 Frame = -2 / -1

                                
Query: 590 KSPATSNFKGWWFVGMGR*ME 528
           +S A + F GWWFV +G *ME
Sbjct: 513 QSYAMTTFMGWWFVEVGT*ME 451




 Score = 30.9 bits (61), Expect(3) = 3e-032
 Identities = 12/20 (60%), Positives = 14/20 (70%)
 Frame = +1 / +2

                               
Query: 529 SIHRPIPTNHQPLKFDVAGD 588
           SIH P  TNHQP+K  +A D
Sbjct: 452 SIHVPTSTNHQPMKVVIA*D 511




 Score = 23.5 bits (45), Expect(4) = 4e-035
 Identities = 7/12 (58%), Positives = 10/12 (83%)
 Frame = -1 / -3

                       
Query: 666 QNKHGTHTENDH 631
           QNKH  HT+N++
Sbjct: 574 QNKHFNHTDNNY 539



  Database: Hordeum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:16 PM
  Number of letters in database: 175,134,539
  Number of sequences in database:  312,970
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 442,521,493
Number of Sequences: 312970
Number of extensions: 9916187
Number of successful extensions: 144934
Number of sequences better than 1.0e-020: 1
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 8653008636
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)