TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAR2a06.yg.3.1
(612 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE213874.2|BE213874 HV_CEb0001I06f Hordeum vulgare seedl... 87 2e-024
gb|BU967692.1|BU967692 HB05B18r BC Hordeum vulgare subsp. v... 78 4e-014
gb|BU986569.1|BU986569 HF12E06r HF Hordeum vulgare subsp. v... 78 4e-014
gb|BG365524.1|BG365524 HVSMEi0003A10f Hordeum vulgare 20 DA... 77 1e-013
gb|BJ549532.1|BJ549532 BJ549532 K. Sato unpublished cDNA li... 64 6e-010
gb|AY946216.1| Hordeum vulgare subsp. vulgare 3' flanking s... 60 1e-008
gb|BJ471441.1|BJ471441 BJ471441 K. Sato unpublished cDNA li... 60 1e-008
gb|AY505378.1| Hordeum vulgare subsp. vulgare cultivar Oreg... 60 1e-008
gb|AY505380.1| Hordeum vulgare subsp. vulgare cultivar Gold... 60 1e-008
gb|AY505379.1| Hordeum vulgare subsp. vulgare cultivar Oreg... 59 2e-008
gb|AV832414.1|AV832414 AV832414 K. Sato unpublished cDNA li... 51 6e-006
gb|BU971420.1|BU971420 HB17J01r BC Hordeum vulgare subsp. v... 49 3e-005
gb|BI949839.1|BI949839 HVSMEl0016G04f Hordeum vulgare spike... 48 4e-005
gb|AY551436.1| Hordeum vulgare subsp. vulgare ent-kaurene s... 48 4e-005
gb|BF617606.2|BF617606 HVSMEc0018E08f Hordeum vulgare seedl... 36 0.20
gb|AV917014.1|AV917014 AV917014 K. Sato unpublished cDNA li... 36 0.20
gb|BJ465148.1|BJ465148 BJ465148 K. Sato unpublished cDNA li... 36 0.20
gb|BU982036.1|BU982036 HA25F11r HA Hordeum vulgare subsp. v... 36 0.20
gb|CA020116.1|CA020116 HV14G03r HV Hordeum vulgare subsp. v... 36 0.20
gb|CA027382.1|CA027382 HZ58L22r HZ Hordeum vulgare subsp. v... 36 0.20
gb|CA027436.1|CA027436 HZ58O16r HZ Hordeum vulgare subsp. v... 36 0.20
gb|BU977582.1|BU977582 HA11K22r HA Hordeum vulgare subsp. v... 29 0.47
gb|BF620481.2|BF620481 HVSMEc0020A13f Hordeum vulgare seedl... 33 1.9
gb|BE422193.1|BE422193 HWM021cD.02r ITEC HWM Barley Leaf Li... 32 3.5
gb|BJ462720.1|BJ462720 BJ462720 K. Sato unpublished cDNA li... 32 3.5
gb|BQ463095.1|BQ463095 HI04A09r HI Hordeum vulgare subsp. v... 32 3.5
gb|BF624529.2|BF624529 HVSMEa0017A16f Hordeum vulgare seedl... 31 4.8
gb|BI946880.1|BI946880 HVSMEl0002K05f Hordeum vulgare spike... 31 4.8
gb|BM369327.2|BM369327 EBem07_SQ003_E06_R embryo, 28 DPA, n... 25 5.4
gb|AY551435.1| Hordeum vulgare subsp. vulgare copalyl dipho... 26 6.3
gb|BF628648.2|BF628648 HVSMEb0006O08f Hordeum vulgare seedl... 31 6.7
gb|BF259174.2|BF259174 HVSMEf0017P17f Hordeum vulgare seedl... 31 6.7
gb|BI952785.1|BI952785 HVSMEm0007M02f Hordeum vulgare green... 31 6.7
gb|BI955720.1|BI955720 HVSMEm0024D12f Hordeum vulgare green... 31 6.7
gb|BI959084.1|BI959084 HVSMEn0018B10f Hordeum vulgare rachi... 31 6.7
gb|BQ467336.1|BQ467336 HS03H02r HS Hordeum vulgare subsp. v... 31 6.7
gb|BM100136.2|BM100136 EBma01_SQ001_E20_R maternal, 4 DPA, ... 31 6.7
gb|CA011985.1|CA011985 HT04B15r HT Hordeum vulgare subsp. v... 31 6.7
gb|BQ755057.1|BQ755057 EBed02_SQ003_J13_R endosperm, 8 DPA,... 25 7.7
gb|BG367403.1|BG367403 HVSMEi0012C09f Hordeum vulgare 20 DA... 30 9.1
gb|BG369394.1|BG369394 HVSMEi0024D17f Hordeum vulgare 20 DA... 30 9.1
gb|AV909006.1|AV909006 AV909006 K. Sato unpublished cDNA li... 30 9.1
gb|BQ462547.1|BQ462547 HI01D21T HI Hordeum vulgare subsp. v... 30 9.1
gb|BM369331.2|BM369331 EBem07_SQ003_E11_R embryo, 28 DPA, n... 30 9.1
gb|CA023634.1|CA023634 HZ46O10r HZ Hordeum vulgare subsp. v... 30 9.1
>gb|BE213874.2|BE213874 HV_CEb0001I06f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0001I06f, mRNA sequence
Length = 918
Score = 87.2 bits (184), Expect(2) = 2e-024
Identities = 37/93 (39%), Positives = 58/93 (62%)
Frame = +1 / +1
Query: 103 AHEVRSSLEIPLPKRGRIYESKYYIHAYETSGTLHEKVLQLAKLNSNVLQLHHQQELKIL 282
A +V+ +L +PL + + E+ +YI Y+ + +L+LAKL+ N+LQ H +ELK L
Sbjct: 301 AEQVKRALRVPLSRTLKRVETLHYISEYKHEERHNPTLLELAKLDFNLLQHVHLKELKYL 480
Query: 283 TRWWEDMQIGSKLPFARDRIVECFLWILGVYYE 381
TRWW D+ L +ARDR+ E ++W VY+E
Sbjct: 481 TRWWHDLYTCVGLNYARDRLGEGYIWCYAVYHE 579
Score = 44.6 bits (91), Expect(2) = 2e-024
Identities = 18/33 (54%), Positives = 23/33 (69%)
Frame = +1 / +1
Query: 1 AHFRTHGETILDEAIAFARSRLEMMLPYVKGPL 99
AH HGET ++EAI+FAR LE+M +K PL
Sbjct: 202 AHLLVHGETSVEEAISFARHHLELMRETLKSPL 300
Score = 29.0 bits (57), Expect(2) = 0.012
Identities = 26/78 (33%), Positives = 34/78 (43%)
Frame = -2 / -2
Query: 335 SRAKGSFDPICISSHHLVRIFSSC**WSCRTLEFSLASCSTFSCSVPLVSYAWM*YFDS* 156
SRA S + S HHLVR SS CR L+ S AS + Y+ +* S
Sbjct: 533 SRA*FSPTHVYKSCHHLVRYLSSFKCTCCRRLKSSFASSNRVGLCRSSCLYSEI*CNVST 354
Query: 155 ILPLLGSGISSEDLTSCA 102
+ +L +G LT A
Sbjct: 353 LFNVLDNGTRRARLTCSA 300
Score = 28.6 bits (56), Expect(2) = 0.012
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = -2 / -2
Query: 110 SCAHNGPFT*GSIISRRLLANAMASSSIVSPW 15
+C+ G I SR LA +ASS+ VSPW
Sbjct: 311 TCSAKGDLRVSLINSR*CLAKEIASSTDVSPW 216
>gb|BU967692.1|BU967692 HB05B18r BC Hordeum vulgare subsp. vulgare cDNA clone HB05B18
5-PRIME, mRNA sequence
Length = 348
Score = 78.0 bits (164), Expect = 4e-014
Identities = 32/107 (29%), Positives = 64/107 (59%)
Frame = +1 / +2
Query: 157 YESKYYIHAYETSGTLHEKVLQLAKLNSNVLQLHHQQELKILTRWWEDMQIGSKLPFARD 336
Y+++Y++ ++ T + + +LA + +L HQ+E++ + RWW D+ + ++ ARD
Sbjct: 26 YKARYHLSYLQSLPTRNTAMEELAVAEFYLNKLQHQKEMREVERWWMDLGLTREIRTARD 205
Query: 337 RIVECFLWILGVYYEPCHSRARIILTMVIAIVTLLDDIFDSFATPQE 477
++++ ++W + V SR RI +T +I+ V + DDIFD T +E
Sbjct: 206 QVLKWYMWAMTVVQGFSLSRYRIEITKIISFVYIXDDIFDLVGTQEE 346
>gb|BU986569.1|BU986569 HF12E06r HF Hordeum vulgare subsp. vulgare cDNA clone HF12E06
5-PRIME, mRNA sequence
Length = 683
Score = 78.0 bits (164), Expect = 4e-014
Identities = 31/93 (33%), Positives = 53/93 (56%)
Frame = +1 / +3
Query: 334 DRIVECFLWILGVYYEPCHSRARIILTMVIAIVTLLDDIFDSFATPQECEILTNCIESWD 513
DR+VEC+ W YYE ++RAR+IL +IAI+ + DD +D AT EC+ L I+ W+
Sbjct: 12 DRVVECYFWAYTAYYEKEYTRARMILAKIIAIIIMTDDTYDVRATLVECKQLNEAIQRWE 191
Query: 514 PKGAQDLPGCMKFALEKILDSYQTITNMLHRED 612
LP ++ K++ +++ + L ++
Sbjct: 192 ESATSLLPEYLQKFYLKLMSTFKEFQDELKPDE 290
Score = 36.8 bits (74), Expect = 0.11
Identities = 25/68 (36%), Positives = 33/68 (48%)
Frame = -2 / -2
Query: 536 GRS*APLGSQLSMQLVSISHSCGVAKESKISSKSVTMAITIVNIIRALE*HGS*YTPKIH 357
GR L S L + +S HS VA+ S +SS + +AI + II A S*Y
Sbjct: 214 GRREVALSSHLCIASLSCLHSTKVARTS*VSSVIIIIAIILARIILARVYSFS*YAVYAQ 35
Query: 356 RKHSTILS 333
+ HST S
Sbjct: 34 K*HSTTRS 11
>gb|BG365524.1|BG365524 HVSMEi0003A10f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0003A10f, mRNA sequence
Length = 743
Score = 76.7 bits (161), Expect = 1e-013
Identities = 32/114 (28%), Positives = 60/114 (52%)
Frame = +1 / +2
Query: 268 ELKILTRWWEDMQIGSKLPFARDRIVECFLWILGVYYEPCHSRARIILTMVIAIVTLLDD 447
E++ + RWW D+ + ++ ARD++++ ++W + V SR RI +T +I+ V ++DD
Sbjct: 2 EMREVERWWMDLGLTREIRTARDQVLKWYMWAMTVVQGFSLSRYRIEITKIISFVYIVDD 181
Query: 448 IFDSFATPQECEILTNCIESWDPKGAQDLPGCMKFALEKILDSYQTITNMLHRE 609
IFD T +E + + I+ WD LP M + + I ++ +E
Sbjct: 182 IFDLVGTQEELSLFSEAIKRWDLAAVGSLPSYMLSCYKALYTVTNEIADVAKKE 343
>gb|BJ549532.1|BJ549532 BJ549532 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags37i03 5', mRNA sequence
Length = 566
Score = 64.3 bits (134), Expect = 6e-010
Identities = 30/91 (32%), Positives = 48/91 (52%)
Frame = +1 / +1
Query: 337 RIVECFLWILGVYYEPCHSRARIILTMVIAIVTLLDDIFDSFATPQECEILTNCIESWDP 516
+I++ +++ + + SR RI +T +IAIV+++DDIFD AT +E T I+ WD
Sbjct: 13 QILKWYMFPMTILQGQSFSRYRIEITKIIAIVSIVDDIFDVIATQEELSRFTEAIKMWDL 192
Query: 517 KGAQDLPGCMKFALEKILDSYQTITNMLHRE 609
A LP M+ + I +M RE
Sbjct: 193 AAAASLPSYMRSCYSALYTITNDIADMAERE 285
>gb|AY946216.1| Hordeum vulgare subsp. vulgare 3' flanking sequence from Ds
insertion in TNP-22
Length = 662
Score = 60.2 bits (125), Expect = 1e-008
Identities = 23/69 (33%), Positives = 42/69 (60%)
Frame = +1 / -1
Query: 286 RWWEDMQIGSKLPFARDRIVECFLWILGVYYEPCHSRARIILTMVIAIVTLLDDIFDSFA 465
RWW D+ + ++ ARD++++ ++W + V SR RI +T +I+ V ++DDIFD
Sbjct: 341 RWWMDLGLTREIRTARDQVLKWYMWAMTVVQGFSLSRYRIEITKIISFVYIVDDIFDLVG 162
Query: 466 TPQECEILT 492
T +E + +
Sbjct: 161 TQEELSLFS 135
>gb|BJ471441.1|BJ471441 BJ471441 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal18g20 5', mRNA sequence
Length = 479
Score = 59.7 bits (124), Expect = 1e-008
Identities = 24/78 (30%), Positives = 42/78 (53%)
Frame = +1 / +2
Query: 358 WILGVYYEPCHSRARIILTMVIAIVTLLDDIFDSFATPQECEILTNCIESWDPKGAQDLP 537
W VY+E + +RI LT + +++L+DD +D+ AT +EC L I+ WD LP
Sbjct: 20 WCYAVYHEKEFALSRIFLTKQLMLISLMDDTYDAHATIEECRQLNAAIQRWDESATSILP 199
Query: 538 GCMKFALEKILDSYQTIT 591
+K +++ ++ T
Sbjct: 200 DYLKRFYTELMRVFKDAT 253
>gb|AY505378.1| Hordeum vulgare subsp. vulgare cultivar Oregon Wolfe Barley
Dominant genomic sequence corresponding to Ds insertion
site in TNP-22
Length = 381
Score = 59.7 bits (124), Expect = 1e-008
Identities = 23/67 (34%), Positives = 41/67 (61%)
Frame = +1 / -3
Query: 286 RWWEDMQIGSKLPFARDRIVECFLWILGVYYEPCHSRARIILTMVIAIVTLLDDIFDSFA 465
RWW D+ + ++ ARD++++ ++W + V SR RI +T +I+ V ++DDIFD
Sbjct: 202 RWWMDLGLTREIRTARDQVLKWYMWAMTVVQGFSLSRYRIEITKIISFVYIVDDIFDLVG 23
Query: 466 TPQECEI 486
T +E +
Sbjct: 22 TQEELSL 2
>gb|AY505380.1| Hordeum vulgare subsp. vulgare cultivar Golden Promise genomic
sequence corresponding to Ds insertion site in TNP-22
Length = 381
Score = 59.7 bits (124), Expect = 1e-008
Identities = 23/67 (34%), Positives = 41/67 (61%)
Frame = +1 / -3
Query: 286 RWWEDMQIGSKLPFARDRIVECFLWILGVYYEPCHSRARIILTMVIAIVTLLDDIFDSFA 465
RWW D+ + ++ ARD++++ ++W + V SR RI +T +I+ V ++DDIFD
Sbjct: 202 RWWMDLGLTREIRTARDQVLKWYMWAMTVVQGFSLSRYRIEITKIISFVYIVDDIFDLVG 23
Query: 466 TPQECEI 486
T +E +
Sbjct: 22 TQEELSL 2
>gb|AY505379.1| Hordeum vulgare subsp. vulgare cultivar Oregon Wolfe Barley
Recessive genomic sequence corresponding to Ds insertion
site in TNP-22
Length = 381
Score = 59.3 bits (123), Expect = 2e-008
Identities = 23/67 (34%), Positives = 41/67 (61%)
Frame = +1 / -3
Query: 286 RWWEDMQIGSKLPFARDRIVECFLWILGVYYEPCHSRARIILTMVIAIVTLLDDIFDSFA 465
RWW D+ + ++ ARD++++ ++W + V SR RI +T +I+ V ++DDIFD
Sbjct: 202 RWWMDLGLTREVRTARDQVLKWYMWAMTVVQGFSLSRYRIEITKIISFVYIVDDIFDLVG 23
Query: 466 TPQECEI 486
T +E +
Sbjct: 22 TQEELSL 2
>gb|AV832414.1|AV832414 AV832414 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare leaves vegetative stage Hordeum vulgare subsp.
vulgare cDNA clone baak12o06, mRNA sequence
Length = 628
Score = 51.0 bits (105), Expect = 6e-006
Identities = 21/86 (24%), Positives = 49/86 (56%)
Frame = +1 / +2
Query: 103 AHEVRSSLEIPLPKRGRIYESKYYIHAYETSGTLHEKVLQLAKLNSNVLQLHHQQELKIL 282
A VR SL+ P Y++++++ ++ T + + +LA + + +L HQ E++ +
Sbjct: 362 ARYVRHSLDHPYHVSLTQYKARHHLSYLQSLPTRNTAMEELALADFQLNKLQHQMEMQEV 541
Query: 283 TRWWEDMQIGSKLPFARDRIVECFLW 360
RWW + + ++P RD+I++ +++
Sbjct: 542 KRWWMGLGLAQEIPATRDQILKWYMF 619
>gb|BU971420.1|BU971420 HB17J01r BC Hordeum vulgare subsp. vulgare cDNA clone HB17J01
5-PRIME, mRNA sequence
Length = 438
Score = 48.7 bits (100), Expect = 3e-005
Identities = 22/73 (30%), Positives = 36/73 (49%)
Frame = +1 / +1
Query: 391 SRARIILTMVIAIVTLLDDIFDSFATPQECEILTNCIESWDPKGAQDLPGCMKFALEKIL 570
SR RI +T +I+ V ++DDIFD T +E + + I+ WD LP M + +
Sbjct: 40 SRYRIEITKIISFVYIVDDIFDLVGTQEELSLFSEAIKRWDLAAVGSLPSYMLSCYKALY 219
Query: 571 DSYQTITNMLHRE 609
I ++ +E
Sbjct: 220 TVTNEIADVAKKE 258
>gb|BI949839.1|BI949839 HVSMEl0016G04f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0016G04f, mRNA sequence
Length = 443
Score = 48.3 bits (99), Expect = 4e-005
Identities = 24/80 (30%), Positives = 35/80 (43%)
Frame = +1 / +2
Query: 292 WEDMQIGSKLPFARDRIVECFLWILGVYYEPCHSRARIILTMVIAIVTLLDDIFDSFATP 471
W +L FAR ++ C+L + P S ARI + T++DD FD +
Sbjct: 200 WVKQNRLDQLQFARQKLTYCYLSAAATIFPPELSDARISWAKNGVLTTVVDDFFDVGGSK 379
Query: 472 QECEILTNCIESWDPKGAQD 531
+E E L +E WD D
Sbjct: 380 EELENLIALVEKWDEHHEDD 439
>gb|AY551436.1| Hordeum vulgare subsp. vulgare ent-kaurene synthase-like protein 1
(KSL1) mRNA, complete cds
Length = 2675
Score = 48.3 bits (99), Expect = 4e-005
Identities = 24/80 (30%), Positives = 35/80 (43%)
Frame = +1 / +3
Query: 292 WEDMQIGSKLPFARDRIVECFLWILGVYYEPCHSRARIILTMVIAIVTLLDDIFDSFATP 471
W +L FAR ++ C+L + P S ARI + T++DD FD +
Sbjct: 1686 WVKQNRLDQLQFARQKLTYCYLSAAATIFPPELSDARISWAKNGVLTTVVDDFFDVGGSK 1865
Query: 472 QECEILTNCIESWDPKGAQD 531
+E E L +E WD D
Sbjct: 1866 EELENLIALVEKWDEHHEDD 1925
>gb|BF617606.2|BF617606 HVSMEc0018E08f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0018E08f, mRNA sequence
Length = 811
Score = 35.9 bits (72), Expect = 0.20
Identities = 15/64 (23%), Positives = 33/64 (51%)
Frame = +1 / +1
Query: 325 FARDRIVECFLWILGVYYEPCHSRARIILTMVIAIVTLLDDIFDSFATPQECEILTNCIE 504
FA + F+W G+ P S A+++ + +AIV + ++F + + + T+ I+
Sbjct: 319 FAVGTTISLFMWATGILKRPKISGAQLLAILPLAIVHTMGNLFTNMSLGKVAVSFTHTIK 498
Query: 505 SWDP 516
+ +P
Sbjct: 499 AMEP 510
>gb|AV917014.1|AV917014 AV917014 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20h11 5', mRNA sequence
Length = 682
Score = 35.9 bits (72), Expect = 0.20
Identities = 15/64 (23%), Positives = 33/64 (51%)
Frame = +1 / +3
Query: 325 FARDRIVECFLWILGVYYEPCHSRARIILTMVIAIVTLLDDIFDSFATPQECEILTNCIE 504
FA + F+W G+ P S A+++ + +AIV + ++F + + + T+ I+
Sbjct: 153 FAVGTTISLFMWATGILKRPKISGAQLLAILPLAIVHTMGNLFTNMSLGKVAVSFTHTIK 332
Query: 505 SWDP 516
+ +P
Sbjct: 333 AMEP 344
>gb|BJ465148.1|BJ465148 BJ465148 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags38c05 5', mRNA sequence
Length = 585
Score = 35.9 bits (72), Expect = 0.20
Identities = 15/64 (23%), Positives = 33/64 (51%)
Frame = +1 / +1
Query: 325 FARDRIVECFLWILGVYYEPCHSRARIILTMVIAIVTLLDDIFDSFATPQECEILTNCIE 504
FA + F+W G+ P S A+++ + +AIV + ++F + + + T+ I+
Sbjct: 130 FAVGTTISLFMWATGILKRPKISGAQLLAILPLAIVHTMGNLFTNMSLGKVAVSFTHTIK 309
Query: 505 SWDP 516
+ +P
Sbjct: 310 AMEP 321
>gb|BU982036.1|BU982036 HA25F11r HA Hordeum vulgare subsp. vulgare cDNA clone HA25F11
5-PRIME, mRNA sequence
Length = 544
Score = 35.9 bits (72), Expect = 0.20
Identities = 15/64 (23%), Positives = 33/64 (51%)
Frame = +1 / +3
Query: 325 FARDRIVECFLWILGVYYEPCHSRARIILTMVIAIVTLLDDIFDSFATPQECEILTNCIE 504
FA + F+W G+ P S A+++ + +AIV + ++F + + + T+ I+
Sbjct: 153 FAVGTTISLFMWATGILKRPKISGAQLLAILPLAIVHTMGNLFTNMSLGKVAVSFTHTIK 332
Query: 505 SWDP 516
+ +P
Sbjct: 333 AMEP 344
>gb|CA020116.1|CA020116 HV14G03r HV Hordeum vulgare subsp. vulgare cDNA clone HV14G03
5-PRIME, mRNA sequence
Length = 669
Score = 35.9 bits (72), Expect = 0.20
Identities = 15/64 (23%), Positives = 33/64 (51%)
Frame = +1 / +1
Query: 325 FARDRIVECFLWILGVYYEPCHSRARIILTMVIAIVTLLDDIFDSFATPQECEILTNCIE 504
FA + F+W G+ P S A+++ + +AIV + ++F + + + T+ I+
Sbjct: 232 FAVGTTISLFMWATGILKRPKISGAQLLAILPLAIVHTMGNLFTNMSLGKVAVSFTHTIK 411
Query: 505 SWDP 516
+ +P
Sbjct: 412 AMEP 423
>gb|CA027382.1|CA027382 HZ58L22r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ58L22
5-PRIME, mRNA sequence
Length = 425
Score = 35.9 bits (72), Expect = 0.20
Identities = 15/64 (23%), Positives = 33/64 (51%)
Frame = +1 / +3
Query: 325 FARDRIVECFLWILGVYYEPCHSRARIILTMVIAIVTLLDDIFDSFATPQECEILTNCIE 504
FA + F+W G+ P S A+++ + +AIV + ++F + + + T+ I+
Sbjct: 159 FAVGTTISLFMWATGILKRPKISGAQLLAILPLAIVHTMGNLFTNMSLGKVAVSFTHTIK 338
Query: 505 SWDP 516
+ +P
Sbjct: 339 AMEP 350
>gb|CA027436.1|CA027436 HZ58O16r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ58O16
5-PRIME, mRNA sequence
Length = 406
Score = 35.9 bits (72), Expect = 0.20
Identities = 15/64 (23%), Positives = 33/64 (51%)
Frame = +1 / +3
Query: 325 FARDRIVECFLWILGVYYEPCHSRARIILTMVIAIVTLLDDIFDSFATPQECEILTNCIE 504
FA + F+W G+ P S A+++ + +AIV + ++F + + + T+ I+
Sbjct: 159 FAVGTTISLFMWATGILKRPKISGAQLLAILPLAIVHTMGNLFTNMSLGKVAVSFTHTIK 338
Query: 505 SWDP 516
+ +P
Sbjct: 339 AMEP 350
>gb|BU977582.1|BU977582 HA11K22r HA Hordeum vulgare subsp. vulgare cDNA clone HA11K22
5-PRIME, mRNA sequence
Length = 590
Score = 29.0 bits (57), Expect(2) = 0.47
Identities = 10/36 (27%), Positives = 20/36 (55%)
Frame = +1 / +3
Query: 427 IVTLLDDIFDSFATPQECEILTNCIESWDPKGAQDL 534
+V L D+ D +P + + + +++WDP+ DL
Sbjct: 450 LVKELFDVIDCMRSPPKKKFMVGPVQNWDPRLEPDL 557
Score = 23.1 bits (44), Expect(2) = 0.47
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = +1 / +1
Query: 370 VYYEPCHSRARIILTMVIAIVTL 438
+Y+ PC + +ILT + IV L
Sbjct: 361 MYFTPCWNNYLLILTSRLIIVLL 429
>gb|BF620481.2|BF620481 HVSMEc0020A13f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0020A13f, mRNA sequence
Length = 896
Score = 32.7 bits (65), Expect = 1.9
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +1 / -1
Query: 58 SRLEMMLPYVKGPLWAHEVRSSLEIPLPKRGR 153
SR+ + V GPLW R++L P P+R R
Sbjct: 572 SRIPRLAAXVNGPLWPRPTRATLLKPCPERRR 477
>gb|BE422193.1|BE422193 HWM021cD.02r ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM021cD.02, mRNA sequence
Length = 995
Score = 31.8 bits (63), Expect = 3.5
Identities = 17/35 (48%), Positives = 18/35 (51%)
Frame = +3 / +1
Query: 495 LHRKLGSQRGSRPPRLHEICSREDIG*LPDHYKYA 599
LHR G R S P L E SR D G LPD + A
Sbjct: 58 LHRGGGRSRTSGSPGLFEFGSRPDAGELPDSCREA 162
>gb|BJ462720.1|BJ462720 BJ462720 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags24b14 5', mRNA sequence
Length = 617
Score = 31.8 bits (63), Expect = 3.5
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = +1 / +3
Query: 1 AHFRTHGETILDEAIAFARSRLE 69
AH RTHGE L A+A+ + L+
Sbjct: 348 AHIRTHGEETLSSAMAYTKDHLQ 416
>gb|BQ463095.1|BQ463095 HI04A09r HI Hordeum vulgare subsp. vulgare cDNA clone HI04A09
5-PRIME, mRNA sequence
Length = 425
Score = 31.8 bits (63), Expect = 3.5
Identities = 19/53 (35%), Positives = 25/53 (47%)
Frame = +3 / +1
Query: 177 PCV*DKWNAA*EGTATCQAELQRSAAPSSARAEDPDKMVGGYADRIKTSFRTR 335
PC +A EG A C A +RS AP++A G A R ++S R R
Sbjct: 196 PCWRASSCSAPEGPAACPARARRSEAPATAACACWPSTAAGPARRTRSSPRPR 354
>gb|BF624529.2|BF624529 HVSMEa0017A16f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0017A16f, mRNA sequence
Length = 908
Score = 31.3 bits (62), Expect = 4.8
Identities = 14/26 (53%), Positives = 16/26 (61%)
Frame = -3 / +1
Query: 121 RI*LHAPTMALSRRAASSPDGFSRTR 44
R+ L P + LSR AA P GFS TR
Sbjct: 826 RLPLVGPMLTLSRSAALKPKGFSNTR 903
>gb|BI946880.1|BI946880 HVSMEl0002K05f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0002K05f, mRNA sequence
Length = 823
Score = 31.3 bits (62), Expect = 4.8
Identities = 12/31 (38%), Positives = 16/31 (51%)
Frame = +1 / +3
Query: 307 IGSKLPFARDRIVECFLWILGVYYEPCHSRA 399
+ K+ F+RD I F W+ G PC S A
Sbjct: 321 VAEKVGFSRDEIQSVFSWLRGFVRSPCGSCA 413
>gb|BM369327.2|BM369327 EBem07_SQ003_E06_R embryo, 28 DPA, no treatment, cv Optic, EBem07
Hordeum vulgare subsp. vulgare cDNA clone
EBem07_SQ003_E06 5', mRNA sequence
Length = 443
Score = 24.9 bits (48), Expect(2) = 5.4
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = +2 / +1
Query: 89 KGHCGRMKSDPRWRSRCLKGAGSTSQSIT 175
+G R + RWRSR GA S ++ T
Sbjct: 61 RGDARRCRRTTRWRSRTWSGARSWRRTRT 147
Score = 24.4 bits (47), Expect(2) = 5.4
Identities = 8/12 (66%), Positives = 9/12 (75%)
Frame = +3 / +2
Query: 576 LPDHYKYAPPRG 611
LPDH + PPRG
Sbjct: 167 LPDHARRPPPRG 202
>gb|AY551435.1| Hordeum vulgare subsp. vulgare copalyl diphosphate synthase-like
protein (CPSL1) mRNA, complete cds
Length = 2635
Score = 25.8 bits (50), Expect(2) = 6.3
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = +1 / +1
Query: 217 LQLAKLNSNVLQLHHQQELKILTRWW 294
L+LAK + N Q+ HQ E L +W+
Sbjct: 1591 LELAKRDFNRCQVQHQLEWHGLQKWF 1668
Score = 25.8 bits (50), Expect(2) = 6.3
Identities = 12/34 (35%), Positives = 16/34 (47%)
Frame = +2 / +2
Query: 50 SREAVWR*CCPT*KGHCGRMKSDPRWRSRCLKGA 151
SR + WR*C + C +K P W R G+
Sbjct: 764 SRGSRWR*CTRFQRRSCIPLKGCPGWTGRKSSGS 865
>gb|BF628648.2|BF628648 HVSMEb0006O08f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0006O08f, mRNA sequence
Length = 698
Score = 30.9 bits (61), Expect = 6.7
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = -1 / +1
Query: 150 APFRQRDLQRGSDFMRPQWPFH 85
+P+R+R L +G D RPQW H
Sbjct: 553 SPWRRRFLWQGPDQSRPQWRLH 618
>gb|BF259174.2|BF259174 HVSMEf0017P17f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0017P17f, mRNA sequence
Length = 958
Score = 30.9 bits (61), Expect = 6.7
Identities = 13/37 (35%), Positives = 16/37 (43%)
Frame = -3 / -2
Query: 229 WQVAVPSHAAFHLSHTHGCNTLTRRSCPF*AAGSPAR 119
W S A F +H H C T + CP A G A+
Sbjct: 417 WLTNATSAAPFSCAHHHACKTPESKVCPCEAEGGHAQ 307
>gb|BI952785.1|BI952785 HVSMEm0007M02f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0007M02f, mRNA sequence
Length = 437
Score = 30.9 bits (61), Expect = 6.7
Identities = 11/32 (34%), Positives = 16/32 (50%)
Frame = +2 / +1
Query: 98 CGRMKSDPRWRSRCLKGAGSTSQSITSMRMRQ 193
C R ++ PRWR +G G T S + R+
Sbjct: 169 CARRRASPRWRPAAGRGRGPTPSSCVARSARR 264
>gb|BI955720.1|BI955720 HVSMEm0024D12f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0024D12f, mRNA sequence
Length = 679
Score = 30.9 bits (61), Expect = 6.7
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = +1 / +1
Query: 1 AHFRTHGETILDEAIAFARSRLE 69
AH RTHGE L A+A+ + L+
Sbjct: 505 AHIRTHGEETLSSAMAYTKDPLQ 573
>gb|BI959084.1|BI959084 HVSMEn0018B10f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0018B10f, mRNA sequence
Length = 636
Score = 30.9 bits (61), Expect = 6.7
Identities = 13/17 (76%), Positives = 13/17 (76%)
Frame = -3 / -2
Query: 91 LSRRAASSPDGFSRTRW 41
L RRAASSP G S TRW
Sbjct: 257 LRRRAASSPPGASWTRW 207
>gb|BQ467336.1|BQ467336 HS03H02r HS Hordeum vulgare subsp. vulgare cDNA clone HS03H02
5-PRIME, mRNA sequence
Length = 357
Score = 30.9 bits (61), Expect = 6.7
Identities = 13/17 (76%), Positives = 13/17 (76%)
Frame = -3 / -3
Query: 91 LSRRAASSPDGFSRTRW 41
L RRAASSP G S TRW
Sbjct: 325 LRRRAASSPPGASWTRW 275
>gb|BM100136.2|BM100136 EBma01_SQ001_E20_R maternal, 4 DPA, no treatment, cv Optic, EBma01
Hordeum vulgare subsp. vulgare cDNA clone
EBma01_SQ001_E20 5', mRNA sequence
Length = 451
Score = 30.9 bits (61), Expect = 6.7
Identities = 15/40 (37%), Positives = 18/40 (45%)
Frame = +2 / +1
Query: 74 CCPT*KGHCGRMKSDPRWRSRCLKGAGSTSQSITSMRMRQ 193
C G C +S R RSR + GS S S S + RQ
Sbjct: 211 CSARIPGRCSLRRSSSRTRSRRRRTRGSASSSYVSQKRRQ 330
>gb|CA011985.1|CA011985 HT04B15r HT Hordeum vulgare subsp. vulgare cDNA clone HT04B15
5-PRIME, mRNA sequence
Length = 490
Score = 30.9 bits (61), Expect = 6.7
Identities = 19/42 (45%), Positives = 22/42 (52%)
Frame = -3 / +1
Query: 175 CNTLTRRSCPF*AAGSPARI*LHAPTMALSRRAASSPDGFSR 50
C+ R C *A +PAR A TM+ SR A SP G SR
Sbjct: 58 CSCAARPICC**ARVTPARPIPTASTMSTSRPARLSPSGGSR 183
>gb|BQ755057.1|BQ755057 EBed02_SQ003_J13_R endosperm, 8 DPA, no treatment, cv Optic, EBed02
Hordeum vulgare subsp. vulgare cDNA clone
EBed02_SQ003_J13 5', mRNA sequence
Length = 632
Score = 24.9 bits (48), Expect(2) = 7.7
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = +2 / +2
Query: 89 KGHCGRMKSDPRWRSRCLKGAGSTSQSIT 175
+G R + RWRSR GA S ++ T
Sbjct: 41 RGDARRCRRTTRWRSRTWSGARSWRRTRT 127
Score = 24.4 bits (47), Expect(2) = 7.7
Identities = 8/12 (66%), Positives = 9/12 (75%)
Frame = +3 / +3
Query: 576 LPDHYKYAPPRG 611
LPDH + PPRG
Sbjct: 147 LPDHARRPPPRG 182
>gb|BG367403.1|BG367403 HVSMEi0012C09f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0012C09f, mRNA sequence
Length = 708
Score = 30.4 bits (60), Expect = 9.1
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = -3 / +1
Query: 67 PDGFSRTRWLHRVLSPHGS*NA 2
P G RW+H V+SP+G NA
Sbjct: 310 PKGV*ENRWIHPVVSPNGKCNA 375
>gb|BG369394.1|BG369394 HVSMEi0024D17f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0024D17f, mRNA sequence
Length = 854
Score = 30.4 bits (60), Expect = 9.1
Identities = 10/45 (22%), Positives = 22/45 (48%)
Frame = +1 / +2
Query: 289 WWEDMQIGSKLPFARDRIVECFLWILGVYYEPCHSRARIILTMVI 423
WW D + P + + + C + + + + C RAR ++ M++
Sbjct: 560 WWRDKTMPISRPLSTECMPTCLVTHMLIQWRLCQIRARNLIEMIL 694
>gb|AV909006.1|AV909006 AV909006 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak11d19 5', mRNA sequence
Length = 596
Score = 30.4 bits (60), Expect = 9.1
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = -3 / +1
Query: 67 PDGFSRTRWLHRVLSPHGS*NA 2
P G RW+H V+SP+G NA
Sbjct: 310 PKGV*ENRWIHPVVSPNGKCNA 375
>gb|BQ462547.1|BQ462547 HI01D21T HI Hordeum vulgare subsp. vulgare cDNA clone HI01D21
5-PRIME, mRNA sequence
Length = 567
Score = 30.4 bits (60), Expect = 9.1
Identities = 13/47 (27%), Positives = 20/47 (42%)
Frame = +2 / +3
Query: 74 CCPT*KGHCGRMKSDPRWRSRCLKGAGSTSQSITSMRMRQVERCMRR 214
CC + G + +S W RC + T + + R+ RC RR
Sbjct: 207 CCRSLAGSSRQSRSRAPWSPRCCTSSPGTPRRRRTFSSRRPTRCARR 347
>gb|BM369331.2|BM369331 EBem07_SQ003_E11_R embryo, 28 DPA, no treatment, cv Optic, EBem07
Hordeum vulgare subsp. vulgare cDNA clone
EBem07_SQ003_E11 5', mRNA sequence
Length = 281
Score = 30.4 bits (60), Expect = 9.1
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +2 / -1
Query: 95 HCGRMKSDPRWRSRCLKG 148
HC R++S RW RC +G
Sbjct: 197 HCCRLRSPARWSGRCRRG 144
>gb|CA023634.1|CA023634 HZ46O10r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ46O10
5-PRIME, mRNA sequence
Length = 454
Score = 30.4 bits (60), Expect = 9.1
Identities = 13/47 (27%), Positives = 20/47 (42%)
Frame = +2 / +3
Query: 74 CCPT*KGHCGRMKSDPRWRSRCLKGAGSTSQSITSMRMRQVERCMRR 214
CC + G + +S W RC + T + + R+ RC RR
Sbjct: 189 CCRSLAGSSRQSRSRAPWSPRCCTSSPGTPRRRRTFSSRRPTRCARR 329
Database: Hordeum_nucl_with_EST.fasta
Posted date: May 2, 2006 2:38 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 323,049,388
Number of Sequences: 312970
Number of extensions: 5066525
Number of successful extensions: 48273
Number of sequences better than 10.0: 63
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6447339768
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)