TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAM10b11.yg.2.1
(554 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AL508426.1|AL508426 AL508426 Hordeum vulgare Barke devel... 199 5e-052
gb|BE195973.3|BE195973 HVSMEh0091A04f Hordeum vulgare 5-45 ... 144 3e-034
gb|BI960553.1|BI960553 HVSMEn0025A05f Hordeum vulgare rachi... 139 1e-032
gb|BM376666.2|BM376666 EBem05_SQ002_N23_R embryo, 14 DPA, n... 139 1e-032
gb|AF023472.1|AF023472 Hordeum vulgare peptide transporter ... 139 1e-032
gb|CA011823.1|CA011823 HT03K10r HT Hordeum vulgare subsp. v... 137 4e-032
gb|BI954149.1|BI954149 HVSMEm0016G07f Hordeum vulgare green... 111 2e-024
gb|BI960365.1|BI960365 HVSMEn0024F19f Hordeum vulgare rachi... 111 2e-024
gb|BI956350.1|BI956350 HVSMEn0002L16f Hordeum vulgare rachi... 73 8e-021
>gb|AL508426.1|AL508426 AL508426 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY08L15V 5',
mRNA sequence
Length = 700
Score = 199 bits (429), Expect(2) = 5e-052
Identities = 76/114 (66%), Positives = 93/114 (81%)
Frame = +2 / +2
Query: 212 PEDDEASSRYTSDGTLDINKQRALKKSTGNWRACFMILGTEFAECLAFYATAKNLVTYLT 391
P+ E SRYT DGT+DIN Q ALK+STGNWRACF ILG EF+EC+ F+A +KNL+TYLT
Sbjct: 77 PQPQEECSRYTGDGTVDINAQPALKQSTGNWRACFFILGAEFSECVCFFAVSKNLITYLT 256
Query: 392 AELGETNIDAATNVSTWIGSCYLTPLISAFLADTYWGRYKTMIVFLLLYIVGML 553
L E+N+ AA NVSTW+GSC+LTP+I AFLAD+YWGRY T++VFL +Y GML
Sbjct: 257 TVLHESNVGAARNVSTWVGSCFLTPVIGAFLADSYWGRYLTILVFLSVYTFGML 418
Score = 148 bits (318), Expect(2) = 7e-037
Identities = 66/114 (57%), Positives = 80/114 (70%)
Frame = -2 / -1
Query: 553 KHPNDVEQEEDYHRLVSSPVRVGQEGADERREVAAANPGGHVRRGIDVGLTKLSREVGDQ 374
+HP V EED + +SSP+RVGQEG+D+RRE AAA+PGGH+ RG DV L + R+VGDQ
Sbjct: 418 EHPECVNGEED*NCQISSPIRVGQEGSDDRREEAAADPGGHIPRGADVALVEHRRKVGDQ 239
Query: 373 VLCGRVKCQAFGELGTQNHEACSPVPGALLQSTLLVDIERSVASVS*GSFIILW 212
VL + AF E GT N EACSPV GALL+S L VDI S+ VS* F+ LW
Sbjct: 238 VLRDGEEAHAFAEFGT*NEEACSPVAGALLESRLCVDINCSITGVS*AFFLWLW 77
Score = 145 bits (311), Expect(2) = 2e-034
Identities = 71/116 (61%), Positives = 82/116 (70%)
Frame = -3 / -2
Query: 552 SIPTM*SKRKTIIVLYLPQYVSAKKALMSGVR*QLPIQVDTFVAASMLVSPSSAVR*VTR 373
SIP +*++RKT IV YLPQ SAKKA M+GVR QLP QVDTF+AA L+S S+ VR*V +
Sbjct: 417 SIPNV*TERKTRIVKYLPQ*ESAKKAPMTGVRKQLPTQVDTFLAAPTLLSWSTVVR*VIK 238
Query: 372 FFAVA*NARHSANSVPRIMKHALQFPVLFFRARCLLISSVPSLVYREEASSSSGAR 205
FF A HS NS PR+ KHALQ PVL RA C LIS+VPS VY E +S G R
Sbjct: 237 FFETAKKHTHSLNSAPRMKKHALQLPVLCLRAGCALISTVPSPVYLEHSSCGCGGR 70
Score = 123 bits (263), Expect = 8e-028
Identities = 63/121 (52%), Positives = 78/121 (64%)
Frame = +1 / +1
Query: 190 ATDSVPGSRG**SFLTIH*RRNARYQQAACSEEEHRELESMLHDSGYRVRRMPGILRDRK 369
A S+P S L IH N+ YQ+ ACS+ EHR+LESML S R++RM +LR +
Sbjct: 55 AEPSLPASTATGRMLKIHR*WNS*YQRTACSQAEHRQLESMLLHSRCRIQRMRVLLRRLE 234
Query: 370 EPGHLPHG*AW*DQHRCRDERVHLDWQLLPHAAHQRLLGRHVLGKIQDDDSLPLALHRWD 549
E HLP+ A +Q R R+E VHL QLLPHA H+ LLGR +LGKI D+ SLPL LH D
Sbjct: 235 ELDHLPYDGAPREQRRRREECVHLGRQLLPHAGHRSLLGRLLLGKIFDNSSLPLRLHIRD 414
Query: 550 A 552
A
Sbjct: 415 A 417
Score = 24.9 bits (48), Expect(2) = 7e-037
Identities = 7/12 (58%), Positives = 11/12 (91%)
Frame = -2 / -1
Query: 211 SQVLNQWPLLIC 176
++++ QWPLLIC
Sbjct: 70 AKMVQQWPLLIC 35
Score = 24.9 bits (48), Expect(2) = 5e-052
Identities = 9/12 (75%), Positives = 11/12 (91%)
Frame = +2 / +2
Query: 173 AADEERPLIQYL 208
AADEERPL+ +L
Sbjct: 32 AADEERPLLNHL 67
Score = 21.7 bits (41), Expect(2) = 2e-034
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = -1 / -3
Query: 197 SVASPHLQLP 168
+VASPHL LP
Sbjct: 56 AVASPHLLLP 27
>gb|BE195973.3|BE195973 HVSMEh0091A04f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0091A04f, mRNA sequence
Length = 942
Score = 144 bits (310), Expect = 3e-034
Identities = 58/115 (50%), Positives = 75/115 (65%)
Frame = +2 / +2
Query: 206 LAPEDDEASSRYTSDGTLDINKQRALKKSTGNWRACFMILGTEFAECLAFYATAKNLVTY 385
LA ++ YT DG++D + +K+ TG W+AC ILG E E LA+Y + NLVTY
Sbjct: 35 LANPEESNQLTYTGDGSVDFSGNPVVKEKTGRWKACPFILGNECCERLAYYGISTNLVTY 214
Query: 386 LTAELGETNIDAATNVSTWIGSCYLTPLISAFLADTYWGRYKTMIVFLLLYIVGM 550
LT +L + N AA NV+TW G+CYLTPLI A LAD YWGRY T+ F +Y +GM
Sbjct: 215 LTKKLHDGNSSAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIATFSTIYFIGM 379
>gb|BI960553.1|BI960553 HVSMEn0025A05f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0025A05f, mRNA sequence
Length = 661
Score = 139 bits (298), Expect = 1e-032
Identities = 56/108 (51%), Positives = 72/108 (66%)
Frame = +2 / +3
Query: 227 ASSRYTSDGTLDINKQRALKKSTGNWRACFMILGTEFAECLAFYATAKNLVTYLTAELGE 406
A+ YT DGT+DI ALKK TGNWRAC IL E E LA+Y + NLV ++ +G
Sbjct: 3 AAEMYTQDGTVDIKGNPALKKDTGNWRACPYILANECCERLAYYGMSTNLVNFMKDRMGM 182
Query: 407 TNIDAATNVSTWIGSCYLTPLISAFLADTYWGRYKTMIVFLLLYIVGM 550
N AA NV+ W G+CY+TPLI AFLAD Y GR+ T+ F+++YI G+
Sbjct: 183 ANAAAANNVTNWGGTCYITPLIGAFLADAYLGRFWTIASFMIIYIFGL 326
>gb|BM376666.2|BM376666 EBem05_SQ002_N23_R embryo, 14 DPA, no treatment, cv Optic, EBem05
Hordeum vulgare subsp. vulgare cDNA clone
EBem05_SQ002_N23 5', mRNA sequence
Length = 554
Score = 139 bits (298), Expect = 1e-032
Identities = 56/108 (51%), Positives = 72/108 (66%)
Frame = +2 / +3
Query: 227 ASSRYTSDGTLDINKQRALKKSTGNWRACFMILGTEFAECLAFYATAKNLVTYLTAELGE 406
A+ YT DGT+DI ALKK TGNWRAC IL E E LA+Y + NLV ++ +G
Sbjct: 108 AAEMYTQDGTVDIKGNPALKKDTGNWRACPYILANECCERLAYYGMSTNLVNFMKDRMGM 287
Query: 407 TNIDAATNVSTWIGSCYLTPLISAFLADTYWGRYKTMIVFLLLYIVGM 550
N AA NV+ W G+CY+TPLI AFLAD Y GR+ T+ F+++YI G+
Sbjct: 288 ANAAAANNVTNWGGTCYITPLIGAFLADAYLGRFWTIASFMIIYIFGL 431
>gb|AF023472.1|AF023472 Hordeum vulgare peptide transporter (ptr1) mRNA, complete cds
Length = 2260
Score = 139 bits (298), Expect = 1e-032
Identities = 56/108 (51%), Positives = 72/108 (66%)
Frame = +2 / +3
Query: 227 ASSRYTSDGTLDINKQRALKKSTGNWRACFMILGTEFAECLAFYATAKNLVTYLTAELGE 406
A+ YT DGT+DI ALKK TGNWRAC IL E E LA+Y + NLV ++ +G
Sbjct: 288 AAEMYTQDGTVDIKGNPALKKDTGNWRACPYILANECCERLAYYGMSTNLVNFMKDRMGM 467
Query: 407 TNIDAATNVSTWIGSCYLTPLISAFLADTYWGRYKTMIVFLLLYIVGM 550
N AA NV+ W G+CY+TPLI AFLAD Y GR+ T+ F+++YI G+
Sbjct: 468 ANAAAANNVTNWGGTCYITPLIGAFLADAYLGRFWTIASFMIIYIFGL 611
>gb|CA011823.1|CA011823 HT03K10r HT Hordeum vulgare subsp. vulgare cDNA clone HT03K10
5-PRIME, mRNA sequence
Length = 574
Score = 137 bits (294), Expect = 4e-032
Identities = 54/101 (53%), Positives = 69/101 (68%)
Frame = +2 / +3
Query: 248 DGTLDINKQRALKKSTGNWRACFMILGTEFAECLAFYATAKNLVTYLTAELGETNIDAAT 427
DG++D + +K+ TG W+AC ILG E E LA+Y + NLVTYLT +L + N AA
Sbjct: 3 DGSVDFSGNPVVKEKTGRWKACPFILGNECCERLAYYGISTNLVTYLTKKLHDGNSSAAR 182
Query: 428 NVSTWIGSCYLTPLISAFLADTYWGRYKTMIVFLLLYIVGM 550
NV+TW G+CYLTPLI A LAD YWGRY T+ F +Y +GM
Sbjct: 183 NVTTWQGTCYLTPLIGAILADAYWGRYWTIATFSTIYFIGM 305
>gb|BI954149.1|BI954149 HVSMEm0016G07f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0016G07f, mRNA sequence
Length = 623
Score = 111 bits (238), Expect = 2e-024
Identities = 43/105 (40%), Positives = 66/105 (62%)
Frame = +2 / +2
Query: 239 YTSDGTLDINKQRALKKSTGNWRACFMILGTEFAECLAFYATAKNLVTYLTAELGETNID 418
YT DG++D+ L+ G W AC I+ E E +A+Y A NL YLT ++ + ++
Sbjct: 128 YTQDGSVDLRGNPVLRSKRGGWTACTFIVVYELFERMAYYGIAANLFIYLTEKMHQGTVE 307
Query: 419 AATNVSTWIGSCYLTPLISAFLADTYWGRYKTMIVFLLLYIVGML 553
A+ NV+ W G+ +LTPL+ A++AD Y GRY T +V +Y++GML
Sbjct: 308 ASNNVTNWSGTVFLTPLLGAYVADAYLGRYWTFVVGSGMYLMGML 442
>gb|BI960365.1|BI960365 HVSMEn0024F19f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0024F19f, mRNA sequence
Length = 644
Score = 111 bits (238), Expect = 2e-024
Identities = 45/124 (36%), Positives = 69/124 (55%)
Frame = +2 / +3
Query: 182 EERPLIQYLAPEDDEASSRYTSDGTLDINKQRALKKSTGNWRACFMILGTEFAECLAFYA 361
E+R + P + Y DG++D+ L+ G W AC I+ E E +A+Y
Sbjct: 57 EDRQGDSHQQPAMENGEGAYAQDGSVDLRGNPVLRSKRGGWTACAFIVVYELFERMAYYG 236
Query: 362 TAKNLVTYLTAELGETNIDAATNVSTWIGSCYLTPLISAFLADTYWGRYKTMIVFLLLYI 541
A NLV Y+T L + ++A+ NV+ W G+ +LTPLI A +AD Y GRY T + +Y+
Sbjct: 237 IASNLVMYMTNRLHQGTVEASNNVTNWSGTVFLTPLIGAVVADAYLGRYWTFVAGSAVYL 416
Query: 542 VGML 553
+GM+
Sbjct: 417 MGMI 428
>gb|BI956350.1|BI956350 HVSMEn0002L16f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0002L16f, mRNA sequence
Length = 882
Score = 73.5 bits (154), Expect(2) = 8e-021
Identities = 30/62 (48%), Positives = 38/62 (61%)
Frame = +2 / +2
Query: 227 ASSRYTSDGTLDINKQRALKKSTGNWRACFMILGTEFAECLAFYATAKNLVTYLTAELGE 406
A+ YT DGT+DI ALKK TGNWRAC IL E E LA+Y + NL+ ++ +G
Sbjct: 86 AAEMYTQDGTVDIKGNPALKKDTGNWRACPYILANECCERLAYYCMSTNLLNFMNDRMGM 265
Query: 407 TN 412
N
Sbjct: 266 PN 271
Score = 46.0 bits (94), Expect(2) = 8e-021
Identities = 17/26 (65%), Positives = 21/26 (80%)
Frame = +2 / +3
Query: 419 AATNVSTWIGSCYLTPLISAFLADTY 496
AA N++ W G+CY+TPLI AF ADTY
Sbjct: 279 AAKNLTNWGGTCYITPLILAFPADTY 356
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 294,693,561
Number of Sequences: 312970
Number of extensions: 5189770
Number of successful extensions: 91320
Number of sequences better than 1.0e-020: 13
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 5725776986
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)