TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAH4a02.xg.2.1
(526 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE420972.1|BE420972 HWM004.E01 ITEC HWM Barley Leaf Libr... 249 9e-066
gb|AV836811.1|AV836811 AV836811 K. Sato unpublished cDNA li... 183 5e-046
gb|BE437755.1|BE437755 SFR007.B08F990617 ITEC SFR Barley Le... 162 1e-039
gb|BI776606.2|BI776606 EBpi07_SQ001_B24_R pistil, 12 DPA, n... 103 1e-038
gb|CA028489.1|CA028489 HZ62C12r HZ Hordeum vulgare subsp. v... 119 6e-037
gb|BI948632.1|BI948632 HVSMEl0010F06f Hordeum vulgare spike... 128 1e-036
gb|BM817330.1|BM817330 HC111G07_T3.ab1 HC Hordeum vulgare s... 130 7e-035
gb|BJ447915.1|BJ447915 BJ447915 K. Sato unpublished cDNA li... 130 6e-034
gb|BM368797.2|BM368797 EBem08_SQ004_K02_R embryo, 40 DPA, n... 123 1e-032
gb|BJ466672.1|BJ466672 BJ466672 K. Sato unpublished cDNA li... 125 2e-032
gb|BJ455660.1|BJ455660 BJ455660 K. Sato unpublished cDNA li... 129 2e-032
gb|BU981962.1|BU981962 HA25B23r HA Hordeum vulgare subsp. v... 82 2e-032
gb|BJ464212.1|BJ464212 BJ464212 K. Sato unpublished cDNA li... 128 3e-029
gb|BF259719.2|BF259719 HVSMEf0020B07f Hordeum vulgare seedl... 127 4e-029
gb|AV917599.1|AV917599 AV917599 K. Sato unpublished cDNA li... 104 3e-028
gb|AV930332.1|AV930332 AV930332 K. Sato unpublished cDNA li... 104 3e-028
gb|AV927973.1|AV927973 AV927973 K. Sato unpublished cDNA li... 104 3e-028
gb|BU988298.1|BU988298 HF17D19r HF Hordeum vulgare subsp. v... 122 1e-027
gb|AV934949.1|AV934949 AV934949 K. Sato unpublished cDNA li... 104 1e-027
gb|CA024671.1|CA024671 HZ49P14r HZ Hordeum vulgare subsp. v... 112 9e-027
gb|CA028812.1|CA028812 HZ63F05r HZ Hordeum vulgare subsp. v... 106 4e-025
gb|AV925177.1|AV925177 AV925177 K. Sato unpublished cDNA li... 110 6e-024
gb|BF267850.2|BF267850 HV_CEa0019B08f Hordeum vulgare seedl... 91 3e-023
gb|BF631424.2|BF631424 HVSMEb0015O10f Hordeum vulgare seedl... 101 2e-021
>gb|BE420972.1|BE420972 HWM004.E01 ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM004.E01, mRNA sequence
Length = 1149
Score = 249 bits (541), Expect = 9e-066
Identities = 100/161 (62%), Positives = 118/161 (73%)
Frame = +1 / +1
Query: 1 RGTXXXXXXXXXXXXXXXRNSDVVEMASCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYW 180
+GT +NSDVVEMASCAPL VNDND++W+PDAIVFNSWQHYGCPNYW
Sbjct: 583 KGTLVAALAEAAFLVGLEKNSDVVEMASCAPLFVNDNDRKWSPDAIVFNSWQHYGCPNYW 762
Query: 181 MLHFFKDSSSATLHPSTIQLPNYDQLVTSAITWNNPHDGNTYMKIKVVNFGSKAVSLNIS 360
MLHFFKDSS AT HP+T+Q+ NYDQ+V SAITW +P D +TY+K KVVNF +AV LNI+
Sbjct: 763 MLHFFKDSSGATFHPTTMQISNYDQMVASAITWQSPKDKSTYLKNKVVNFRXQAVDLNIT 942
Query: 361 VTGLETDIQTFGSIKTVLTSGWLRDENSFQQPDKVVPAASP 483
VTGL + I++ GS KTVLT +ENS P KV P SP
Sbjct: 943 VTGLXSGIKSSGSTKTVLTFXAPVEENSSSHPQKVAPVXSP 1065
Score = 205 bits (444), Expect = 8e-053
Identities = 101/160 (63%), Positives = 114/160 (71%)
Frame = -3 / -2
Query: 482 GLAAGTTLSGC*KEFSSRSQPDVRTVFMDPNV*ISVSNPVTDIFRLTALLPKFTTFIFM* 303
GL G T G +EFSS +V TVF++P + + + +PVT + R TA KFTT F *
Sbjct: 1064 GLXTGATFWGWLEEFSSTGAXNVSTVFVEPELLMPLXSPVTVMLRSTACXRKFTTLFFR* 885
Query: 302 VFPSCGLFQVIADVTSWS*FGS*IVDG*SVALLESLKKCSIQ*FGHP*CCHELKTIASGF 123
V S GL QV+AD T S* +V G* VA LESLKKCSIQ*FGHP*CCHEL T+ASG
Sbjct: 884 VLLSLGLCQVMADATI*S*LEICMVVG*KVAPLESLKKCSIQ*FGHP*CCHELNTMASGL 705
Query: 122 HLWSLSFTRSGAQLAISTTSLFLSNPMRNAASAKAAISVP 3
HL SLSFT+SGAQLAISTTSLF SNP RNAASA AA SVP
Sbjct: 704 HLRSLSFTKSGAQLAISTTSLFFSNPTRNAASASAATSVP 585
Score = 114 bits (244), Expect = 3e-025
Identities = 52/143 (36%), Positives = 83/143 (58%)
Frame = -2 / -1
Query: 465 HLVGLLKGVLIPQPTRRENRLYGSKCLNISFQPSYRYIQTNSFASKIYNLYFHVSVPIMW 286
HL+G+ GVL ++ + L+ + Q RY++ + ++++L F VS +
Sbjct: 1047 HLLGVAGGVLFDGRXECQHGFCRAGALDAAXQSGDRYVEIHGLLXEVHHLIFQVSALVFR 868
Query: 285 VIPSDSRCDQLVVVR*LNSGRMKCGAA*IFEKVQHPVIWTSIVLP*VKDYSIWVPPLVIV 106
+P D RC+ L+VV L+ +K AA + E+V HPV+W S+VLP*V+ I P +IV
Sbjct: 867 ALPGDGRCNHLIVVGDLHGCGVKGCAARVLEEV*HPVVWASVVLP*VEHNGIRAPLAIIV 688
Query: 105 VYKEWSTTCHLNHVAVPF*SNEK 37
+Y++ CHL++V V *SNEK
Sbjct: 687 IYEKRCAACHLDYVTVLL*SNEK 619
Score = 108 bits (231), Expect = 2e-023
Identities = 62/155 (40%), Positives = 88/155 (56%)
Frame = +3 / +3
Query: 3 RNTNSCFS*SCISHWIRKEQRRG*DGKLCSTPCKRQ*PKVEPRCYSL*LMAALWMSKLLD 182
RNT S SC+S WIR+EQ R DGKL + K Q* +VEP C+ + LMAAL M KLLD
Sbjct: 585 RNTGSSTRRSCVSRWIREEQ*RSRDGKLRTAFRK*Q*SQVEPGCHCVQLMAALRMPKLLD 764
Query: 183 AALFQRFKQRHTSSVHYSTTELRPTGHICYHLE*PT*WEHLHENKGCKFWKQSC*SEYIC 362
A L Q ++R+ S +++ +LR G IC+HL P EHL E G + S S++
Sbjct: 765 ATLLQGLERRNLSPHNHADLQLRSDGCICHHLAEP*RQEHLPEK*GGELPXASRGSQHNG 944
Query: 363 NWVGN*YSDIWIHKDGSHVGLAAG*ELLSTARQGG 467
+ G + ++ + K+ + + A ELL +GG
Sbjct: 945 HRTGERHQELRLDKNRADIRRARRRELLQPPPKGG 1049
>gb|AV836811.1|AV836811 AV836811 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd3o13, mRNA
sequence
Length = 556
Score = 183 bits (397), Expect = 5e-046
Identities = 77/109 (70%), Positives = 90/109 (82%)
Frame = +1 / -1
Query: 196 KDSSSATLHPSTIQLPNYDQLVTSAITWNNPHDGNTYMKIKVVNFGSKAVSLNISVTGLE 375
+ S A LHPSTIQ+ NY+QLV SAITW N DGN Y+KIKV+NFG+KAV L+IS+TGLE
Sbjct: 553 RTQSGAALHPSTIQVSNYNQLVASAITWQNSKDGNAYLKIKVLNFGNKAVDLSISITGLE 374
Query: 376 TDIQTFGSIKTVLTSGWLRDENSFQQPDKVVPAASPITNAGKQMGVILN 522
IQTFGSIKTVLTSG L DENSFQQPDKVVP SPITNA ++M ++L+
Sbjct: 373 NGIQTFGSIKTVLTSGSLWDENSFQQPDKVVPVESPITNAREEMSLVLD 227
Score = 145 bits (312), Expect = 1e-034
Identities = 68/97 (70%), Positives = 75/97 (77%)
Frame = -3 / +3
Query: 494 ALVIGLAAGTTLSGC*KEFSSRSQPDVRTVFMDPNV*ISVSNPVTDIFRLTALLPKFTTF 315
ALVIGL+ GTTLSGC KEFSS +PDV TVFM+PNV*I S+PV +I R TALLPKF TF
Sbjct: 255 ALVIGLSTGTTLSGCWKEFSSHREPDVSTVFMEPNV*IPFSSPVIEILRSTALLPKFRTF 434
Query: 314 IFM*VFPSCGLFQVIADVTSWS*FGS*IVDG*SVALL 204
IF * FPS QVIAD TSW *F +*+VDG*S A L
Sbjct: 435 IFR*AFPSLEFCQVIADATSWL*FDT*MVDG*SAAPL 545
Score = 143 bits (309), Expect = 3e-034
Identities = 62/104 (59%), Positives = 75/104 (72%)
Frame = -2 / +2
Query: 525 RVQYNTHLLARVGYWTCRRHHLVGLLKGVLIPQPTRRENRLYGSKCLNISFQPSYRYIQT 346
R+QY THLL R+GY RHHLV LL+G+LIP TR + RLYG+KCLN QP YR +
Sbjct: 224 RIQYKTHLLPRIGYRAFHRHHLVWLLEGILIP*RTRCKYRLYGAKCLNPVLQPGYRDTKI 403
Query: 345 NSFASKIYNLYFHVSVPIMWVIPSDSRCDQLVVVR*LNSGRMKC 214
SF ++I NLYF VS+ I ++PSDSRCDQLV+VR LN MKC
Sbjct: 404 YSFVAEI*NLYF*VSISIFRILPSDSRCDQLVIVRYLNGRWMKC 535
Score = 111 bits (239), Expect = 2e-024
Identities = 55/106 (51%), Positives = 66/106 (62%)
Frame = +2 / -2
Query: 200 IQAAPHFIRPLFNYRTTTNWSHLLSLGITHMMGTLT*K*RL*ILEAKLLV*IYL*LGWKL 379
++ HFI F YRT TNWSH LSLG M LT*K*R *I KL + + L* GW+
Sbjct: 549 LKVVQHFIHLPFKYRTITNWSHRLSLGRILKMEMLT*K*RF*ISATKL*ILVSL*PGWRT 370
Query: 380 IFRHLDP*RRFSRRVGCGMRTPFNSPTRWCRRQVQ*PTRASKWVLY 517
FRHL P*RR+ V GMR P ++ TRWCR + +*P R + VLY
Sbjct: 369 GFRHLAP*RRYLHLVLYGMRIPSSNQTRWCRWKAR*PMRGRR*VLY 232
Score = 87.2 bits (185), Expect(2) = 2e-021
Identities = 36/66 (54%), Positives = 46/66 (69%)
Frame = +3 / -3
Query: 294 WEHLHENKGCKFWKQSC*SEYICNWVGN*YSDIWIHKDGSHVGLAAG*ELLSTARQGGAG 473
W+ L +NKG KF +QSC S Y+ N G SDIW+HKDG+++ + G*E L RQGGAG
Sbjct: 455 WKCLPKNKGSKFRQQSCRS*YLYNRAGERDSDIWLHKDGTYIWFSMG*EFLPATRQGGAG 276
Query: 474 GKSNNQ 491
GK +NQ
Sbjct: 275 GKPDNQ 258
Score = 33.5 bits (67), Expect(2) = 2e-021
Identities = 13/21 (61%), Positives = 17/21 (80%)
Frame = +3 / -3
Query: 216 TSSVHYSTTELRPTGHICYHL 278
TSS+++S+ EL PTG I YHL
Sbjct: 533 TSSIYHSSIEL*PTGRIGYHL 471
>gb|BE437755.1|BE437755 SFR007.B08F990617 ITEC SFR Barley Leaf Epidermis Library Hordeum
vulgare subsp. vulgare cDNA clone SF, mRNA sequence
Length = 522
Score = 162 bits (351), Expect = 1e-039
Identities = 68/93 (73%), Positives = 79/93 (84%)
Frame = +1 / +1
Query: 244 NYDQLVTSAITWNNPHDGNTYMKIKVVNFGSKAVSLNISVTGLETDIQTFGSIKTVLTSG 423
NY+QLV SAITW N DGN Y+KIKV+NFG+KAV L+IS+TGLE IQTFGSIKTVLTSG
Sbjct: 4 NYNQLVASAITWQNSKDGNAYLKIKVLNFGNKAVDLSISITGLENGIQTFGSIKTVLTSG 183
Query: 424 WLRDENSFQQPDKVVPAASPITNAGKQMGVILN 522
L DENSFQQPDKVVP SPITNA ++M ++L+
Sbjct: 184 SLWDENSFQQPDKVVPVESPITNAREEMSLVLD 282
Score = 134 bits (289), Expect = 2e-031
Identities = 57/95 (60%), Positives = 70/95 (73%)
Frame = -2 / -1
Query: 525 RVQYNTHLLARVGYWTCRRHHLVGLLKGVLIPQPTRRENRLYGSKCLNISFQPSYRYIQT 346
R+QY THLL R+GY RHHLV LL+G+LIP TR + RLYG+KCLN QP YR +
Sbjct: 285 RIQYKTHLLPRIGYRAFHRHHLVWLLEGILIP*RTRCKYRLYGAKCLNPVLQPGYRDTKI 106
Query: 345 NSFASKIYNLYFHVSVPIMWVIPSDSRCDQLVVVR 241
SF ++I NLYF VS+ I ++PSDSRCDQLV+VR
Sbjct: 105 YSFVAEI*NLYF*VSISIFRILPSDSRCDQLVIVR 1
Score = 132 bits (284), Expect = 1e-030
Identities = 60/84 (71%), Positives = 65/84 (77%)
Frame = -3 / -2
Query: 494 ALVIGLAAGTTLSGC*KEFSSRSQPDVRTVFMDPNV*ISVSNPVTDIFRLTALLPKFTTF 315
ALVIGL+ GTTLSGC KEFSS +PDV TVFM+PNV*I S+PV +I R TALLPKF TF
Sbjct: 254 ALVIGLSTGTTLSGCWKEFSSHREPDVSTVFMEPNV*IPFSSPVIEILRSTALLPKFRTF 75
Query: 314 IFM*VFPSCGLFQVIADVTSWS*F 243
IF * FPS QVIAD TSW *F
Sbjct: 74 IFR*AFPSLEFCQVIADATSWL*F 3
Score = 99.5 bits (212), Expect = 8e-021
Identities = 50/92 (54%), Positives = 59/92 (64%)
Frame = +2 / +2
Query: 242 RTTTNWSHLLSLGITHMMGTLT*K*RL*ILEAKLLV*IYL*LGWKLIFRHLDP*RRFSRR 421
RT TNWSH LSLG M LT*K*R *I KL + + L* GW+ FRHL P*RR+
Sbjct: 2 RTITNWSHRLSLGRILKMEMLT*K*RF*ISATKL*ILVSL*PGWRTGFRHLAP*RRYLHL 181
Query: 422 VGCGMRTPFNSPTRWCRRQVQ*PTRASKWVLY 517
V GMR P ++ TRWCR + +*P R + VLY
Sbjct: 182 VLYGMRIPSSNQTRWCRWKAR*PMRGRR*VLY 277
>gb|BI776606.2|BI776606 EBpi07_SQ001_B24_R pistil, 12 DPA, no treatment, cv Optic, EBpi07
Hordeum vulgare subsp. vulgare cDNA clone
EBpi07_SQ001_B24 5', mRNA sequence
Length = 453
Score = 103 bits (221), Expect(2) = 1e-038
Identities = 38/71 (53%), Positives = 48/71 (67%)
Frame = +1 / +2
Query: 82 SCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYWMLHFFKDSSSATLHPSTIQLPNYDQLV 261
S APL +N+ND++W PDAIVFNSWQ YG P+YWM FF++SS A +HP + L
Sbjct: 5 SYAPLFLNENDRQWTPDAIVFNSWQQYGTPSYWMHTFFRESSGAVIHPMRLNSSYSGSLA 184
Query: 262 TSAITWNNPHD 294
SAITW + D
Sbjct: 185 ASAITWGDSED 217
Score = 75.8 bits (160), Expect(2) = 1e-038
Identities = 33/72 (45%), Positives = 47/72 (65%)
Frame = +1 / +2
Query: 304 YMKIKVVNFGSKAVSLNISVTGLETDIQTFGSIKTVLTSGWLRDENSFQQPDKVVPAASP 483
+++IK+VNFGS AV+L +S GL+ + T S TVLTS + DEN+F P VVP S
Sbjct: 221 FLRIKIVNFGSNAVNLTLSAIGLQAGVNT*RSAVTVLTSDNVLDENTFDDPRNVVPERSD 400
Query: 484 ITNAGKQMGVIL 519
+ NA ++M +L
Sbjct: 401 LPNAAEEMQAML 436
>gb|CA028489.1|CA028489 HZ62C12r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ62C12
5-PRIME, mRNA sequence
Length = 557
Score = 119 bits (256), Expect(3) = 6e-037
Identities = 45/79 (56%), Positives = 55/79 (69%)
Frame = +1 / +1
Query: 58 NSDVVEMASCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYWMLHFFKDSSSATLHPSTIQ 237
NSD+V MAS APL VN+ND++W PDAIVFNSWQ YG P+YWM FF++SS A +HP +
Sbjct: 205 NSDIVHMASYAPLFVNENDRQWTPDAIVFNSWQQYGTPSYWMQTFFRESSGAVIHPMRLN 384
Query: 238 LPNYDQLVTSAITWNNPHD 294
L SAITW + D
Sbjct: 385 SSYSGSLAASAITWGDNED 441
Score = 93.1 bits (198), Expect(2) = 3e-023
Identities = 46/70 (65%), Positives = 49/70 (70%)
Frame = -3 / -1
Query: 212 ALLESLKKCSIQ*FGHP*CCHELKTIASGFHLWSLSFTRSGAQLAISTTSLFLSNPMRNA 33
A L+S K IQ* G P CC ELKTIASG H S SFT+SGA LA+ T SLF SNPMRNA
Sbjct: 359 APLDSRKNVCIQ*LGVPYCCQELKTIASGVHWRSFSFTKSGA*LAMWTISLFTSNPMRNA 180
Query: 32 ASAKAAISVP 3
ASA A VP
Sbjct: 179 ASANEARRVP 150
Score = 44.9 bits (92), Expect(3) = 6e-037
Identities = 18/37 (48%), Positives = 26/37 (70%)
Frame = +1 / +1
Query: 304 YMKIKVVNFGSKAVSLNISVTGLETDIQTFGSIKTVL 414
+++IK+VNFGS AV+L +S GL+ + T S TVL
Sbjct: 445 FLRIKIVNFGSNAVNLTLSAIGLQAGVNTSRSAVTVL 555
Score = 33.9 bits (68), Expect(2) = 3e-023
Identities = 16/35 (45%), Positives = 20/35 (57%)
Frame = -3 / -1
Query: 416 VRTVFMDPNV*ISVSNPVTDIFRLTALLPKFTTFI 312
VRTV + +V +P+ D R TA PKFT FI
Sbjct: 557 VRTVTAERDVLTPACSPIADKVRFTAFDPKFTIFI 453
Score = 27.1 bits (53), Expect(3) = 6e-037
Identities = 10/18 (55%), Positives = 11/18 (61%)
Frame = +3 / +3
Query: 6 NTNSCFS*SCISHWIRKE 59
N +S SCI HWIR E
Sbjct: 153 NPSSFIGRSCIPHWIRSE 206
>gb|BI948632.1|BI948632 HVSMEl0010F06f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0010F06f, mRNA sequence
Length = 817
Score = 128 bits (275), Expect(2) = 1e-036
Identities = 51/95 (53%), Positives = 59/95 (62%)
Frame = +1 / +1
Query: 1 RGTXXXXXXXXXXXXXXXRNSDVVEMASCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYW 180
RG+ +NSD+V MAS APL VNDNDQ WNPDAIVFNSWQ YG P+YW
Sbjct: 187 RGSLLGSLAEAAFLTGLEKNSDIVHMASYAPLFVNDNDQTWNPDAIVFNSWQQYGTPSYW 366
Query: 181 MLHFFKDSSSATLHPSTIQLPNYDQLVTSAITWNN 285
M FF++SS A +HP TI L SAITW +
Sbjct: 367 MQKFFRESSGAMIHPITISSSYSGSLAASAITWQD 471
Score = 94.5 bits (201), Expect(3) = 3e-024
Identities = 48/77 (62%), Positives = 55/77 (71%)
Frame = -3 / -3
Query: 233 IVDG*SVALLESLKKCSIQ*FGHP*CCHELKTIASGFHLWSLSFTRSGAQLAISTTSLFL 54
IV G* +A L+S K IQ* G P CC ELKTIASGFH+ SLSFT+SGA LAI T SLF
Sbjct: 419 IVIG*IMAPLDSRKNFCIQ*LGVPYCCQELKTIASGFHV*SLSFTKSGA*LAI*TISLFF 240
Query: 53 SNPMRNAASAKAAISVP 3
S+P+R AASA +P
Sbjct: 239 SSPVRKAASASEPRRLP 189
Score = 44.4 bits (91), Expect(2) = 1e-036
Identities = 18/34 (52%), Positives = 24/34 (70%)
Frame = +1 / +1
Query: 295 GNTYMKIKVVNFGSKAVSLNISVTGLETDIQTFG 396
GN ++++K VNFGS VSL ISV+GL I+ G
Sbjct: 475 GNNFLRVKDVNFGSDTVSLTISVSGLPASIKRAG 576
Score = 30.7 bits (61), Expect(3) = 3e-024
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = -3 / -3
Query: 371 NPVTDIFRLTALLPKFTTFIFM*VFP 294
+P T+I RLT PKFT+F +FP
Sbjct: 551 SPDTEIVRLTVSDPKFTSFTLRKLFP 474
Score = 22.5 bits (43), Expect(3) = 3e-024
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = -3 / -2
Query: 470 GTTLSGC*KEFSSRSQPDVRTVFMDPN 390
G L G K+FSS + VR V DP+
Sbjct: 651 GHALGGVLKKFSSFTCWXVRPVAFDPS 571
>gb|BM817330.1|BM817330 HC111G07_T3.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC111G07_T3.ab1 similar to (AC011708) putative
alpha-L-arabinofuranosidase [Arabidopsis thaliana], mRNA
sequence
Length = 924
Score = 130 bits (280), Expect = 6e-030
Identities = 54/120 (45%), Positives = 71/120 (59%)
Frame = +1 / +3
Query: 1 RGTXXXXXXXXXXXXXXXRNSDVVEMASCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYW 180
RG+ +NSD+V MAS APL VNDNDQ WNPDAIVFNSWQ YG P+YW
Sbjct: 204 RGSLIGSLAEAAFLTGLEKNSDIVHMASYAPLFVNDNDQTWNPDAIVFNSWQQYGTPSYW 383
Query: 181 MLHFFKDSSSATLHPSTIQLPNYDQLVTSAITWNNPHDGNTYMKIKVVNFGSKAVSLNIS 360
M FF++SS A +HP TI L SAITW + + +KI + ++++++S
Sbjct: 384 MQKFFRESSGAMIHPITISSSYSGSLAASAITWQDSGNNFLRVKIVNFGSDTVSLTISVS 563
Score = 97.7 bits (208), Expect(2) = 7e-035
Identities = 49/77 (63%), Positives = 56/77 (72%)
Frame = -3 / -3
Query: 233 IVDG*SVALLESLKKCSIQ*FGHP*CCHELKTIASGFHLWSLSFTRSGAQLAISTTSLFL 54
IV G* +A L+S K IQ* G P CC ELKTIASGFH+ SLSFT+SGA LAI T SLF
Sbjct: 436 IVIG*IMAPLDSRKNFCIQ*LGVPYCCQELKTIASGFHV*SLSFTKSGA*LAI*TISLFF 257
Query: 53 SNPMRNAASAKAAISVP 3
S+P+R AASA I +P
Sbjct: 256 SSPVRKAASASEPIRLP 206
Score = 96.3 bits (205), Expect(2) = 2e-032
Identities = 41/75 (54%), Positives = 52/75 (69%)
Frame = +1 / +3
Query: 295 GNTYMKIKVVNFGSKAVSLNISVTGLETDIQTFGSIKTVLTSGWLRDENSFQQPDKVVPA 474
GN ++++K+VNFGS VSL ISV+GL+ I GS TVLTS ++DENSF P KVVP
Sbjct: 492 GNNFLRVKIVNFGSDTVSLTISVSGLQASINALGSNATVLTSSNVKDENSFSNPTKVVPV 671
Query: 475 ASPITNAGKQMGVIL 519
S + NA +QM V L
Sbjct: 672 TSQLHNAAEQMQVTL 716
Score = 68.1 bits (143), Expect(2) = 7e-035
Identities = 36/75 (48%), Positives = 44/75 (58%)
Frame = -3 / -3
Query: 518 NITPICLPALVIGLAAGTTLSGC*KEFSSRSQPDVRTVFMDPNV*ISVSNPVTDIFRLTA 339
++T IC AL L GTTL G KEFSS + DVRTV DP+ + +P T+I RLT
Sbjct: 715 SVTCICSAALCN*LVTGTTLVGLLKEFSSFTLLDVRTVAFDPSALMLAWSPDTEIVRLTV 536
Query: 338 LLPKFTTFIFM*VFP 294
PKFT F +FP
Sbjct: 535 SDPKFTIFTLRKLFP 491
Score = 62.2 bits (130), Expect(2) = 2e-032
Identities = 32/67 (47%), Positives = 38/67 (56%)
Frame = +3 / +2
Query: 78 GKLCSTPCKRQ*PKVEPRCYSL*LMAALWMSKLLDAALFQRFKQRHTSSVHYSTTELRPT 257
GKLC+ CKRQ VE RCY L L+A + S LLDA +F R H SS + L +
Sbjct: 281 GKLCTALCKRQRSNVESRCYRLQLLATIRNS*LLDAEVFPRI*WCHDSSNYNQFQLLWFS 460
Query: 258 GHICYHL 278
ICYHL
Sbjct: 461 SSICYHL 481
>gb|BJ447915.1|BJ447915 BJ447915 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak17l17 5', mRNA sequence
Length = 712
Score = 130 bits (280), Expect = 6e-030
Identities = 54/120 (45%), Positives = 71/120 (59%)
Frame = +1 / +1
Query: 1 RGTXXXXXXXXXXXXXXXRNSDVVEMASCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYW 180
RG+ +NSD+V MAS APL VNDNDQ WNPDAIVFNSWQ YG P+YW
Sbjct: 58 RGSLLGSLAEAAFLTGLEKNSDIVHMASYAPLFVNDNDQTWNPDAIVFNSWQQYGTPSYW 237
Query: 181 MLHFFKDSSSATLHPSTIQLPNYDQLVTSAITWNNPHDGNTYMKIKVVNFGSKAVSLNIS 360
M FF++SS A +HP TI L SAITW + + +KI + ++++++S
Sbjct: 238 MQKFFRESSGAMIHPITISSSYSGSLAASAITWQDSGNNFLRVKIVNFGSDTVSLTISVS 417
Score = 96.3 bits (205), Expect(2) = 2e-032
Identities = 41/75 (54%), Positives = 52/75 (69%)
Frame = +1 / +1
Query: 295 GNTYMKIKVVNFGSKAVSLNISVTGLETDIQTFGSIKTVLTSGWLRDENSFQQPDKVVPA 474
GN ++++K+VNFGS VSL ISV+GL+ I GS TVLTS ++DENSF P KVVP
Sbjct: 346 GNNFLRVKIVNFGSDTVSLTISVSGLQASINALGSNATVLTSSNVKDENSFSNPTKVVPV 525
Query: 475 ASPITNAGKQMGVIL 519
S + NA +QM V L
Sbjct: 526 TSQLHNAAEQMQVTL 570
Score = 94.5 bits (201), Expect(2) = 6e-034
Identities = 48/77 (62%), Positives = 55/77 (71%)
Frame = -3 / -3
Query: 233 IVDG*SVALLESLKKCSIQ*FGHP*CCHELKTIASGFHLWSLSFTRSGAQLAISTTSLFL 54
IV G* +A L+S K IQ* G P CC ELKTIASGFH+ SLSFT+SGA LAI T SLF
Sbjct: 290 IVIG*IMAPLDSRKNFCIQ*LGVPYCCQELKTIASGFHV*SLSFTKSGA*LAI*TISLFF 111
Query: 53 SNPMRNAASAKAAISVP 3
S+P+R AASA +P
Sbjct: 110 SSPVRKAASASEPRRLP 60
Score = 68.1 bits (143), Expect(2) = 6e-034
Identities = 36/75 (48%), Positives = 44/75 (58%)
Frame = -3 / -3
Query: 518 NITPICLPALVIGLAAGTTLSGC*KEFSSRSQPDVRTVFMDPNV*ISVSNPVTDIFRLTA 339
++T IC AL L GTTL G KEFSS + DVRTV DP+ + +P T+I RLT
Sbjct: 569 SVTCICSAALCN*LVTGTTLVGLLKEFSSFTLLDVRTVAFDPSALMLAWSPDTEIVRLTV 390
Query: 338 LLPKFTTFIFM*VFP 294
PKFT F +FP
Sbjct: 389 SDPKFTIFTLRKLFP 345
Score = 62.2 bits (130), Expect(2) = 2e-032
Identities = 32/67 (47%), Positives = 38/67 (56%)
Frame = +3 / +3
Query: 78 GKLCSTPCKRQ*PKVEPRCYSL*LMAALWMSKLLDAALFQRFKQRHTSSVHYSTTELRPT 257
GKLC+ CKRQ VE RCY L L+A + S LLDA +F R H SS + L +
Sbjct: 135 GKLCTALCKRQRSNVESRCYRLQLLATIRNS*LLDAEVFPRI*WCHDSSNYNQFQLLWFS 314
Query: 258 GHICYHL 278
ICYHL
Sbjct: 315 SSICYHL 335
>gb|BM368797.2|BM368797 EBem08_SQ004_K02_R embryo, 40 DPA, no treatment, cv Optic, EBem08
Hordeum vulgare subsp. vulgare cDNA clone
EBem08_SQ004_K02 5', mRNA sequence
Length = 479
Score = 123 bits (265), Expect(2) = 1e-032
Identities = 48/87 (55%), Positives = 62/87 (71%)
Frame = +1 / +3
Query: 55 RNSDVVEMASCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYWMLHFFKDSSSATLHPSTI 234
+NSDVV+MAS APL +NDND+ WNPDAIVFNSWQ YG P+YWM F++SS AT+HP ++
Sbjct: 39 KNSDVVQMASYAPLFINDNDRTWNPDAIVFNSWQQYGTPSYWMQTLFRESSGATVHPISL 218
Query: 235 QLPNYDQLVTSAITWNNPHDGNTYMKI 315
L SAITW++ + +KI
Sbjct: 219 SSSCSGLLAASAITWHDDENSFLRVKI 299
Score = 92.7 bits (197), Expect(2) = 6e-031
Identities = 45/67 (67%), Positives = 51/67 (76%)
Frame = -3 / -2
Query: 224 G*SVALLESLKKCSIQ*FGHP*CCHELKTIASGFHLWSLSFTRSGAQLAISTTSLFLSNP 45
G +VA L+S K IQ* G P CC ELKTIASGFH+ SLSF +SGA LAI TTSLF S+P
Sbjct: 208 GWTVAPLDSRKSVCIQ*LGVPYCCQELKTIASGFHVRSLSFMKSGA*LAI*TTSLFFSSP 29
Query: 44 MRNAASA 24
+R AASA
Sbjct: 28 VRKAASA 8
Score = 84.9 bits (180), Expect(2) = 6e-027
Identities = 35/70 (50%), Positives = 47/70 (67%)
Frame = +1 / +3
Query: 292 DGNTYMKIKVVNFGSKAVSLNISVTGLETDIQTFGSIKTVLTSGWLRDENSFQQPDKVVP 471
D N+++++K+VNFG AV L IS TGL+ I GS TVLTSG + DENSF P+KVVP
Sbjct: 270 DENSFLRVKIVNFGPDAVGLTISATGLQGSINALGSTATVLTSGGVMDENSFANPNKVVP 449
Query: 472 AASPITNAGK 501
+ +A +
Sbjct: 450 VTVELRDAAE 479
Score = 59.9 bits (125), Expect(2) = 6e-031
Identities = 30/60 (50%), Positives = 38/60 (63%)
Frame = -3 / -2
Query: 470 GTTLSGC*KEFSSRSQPDVRTVFMDPNV*ISVSNPVTDIFRLTALLPKFTTFIFM*VFPS 291
GTTL G KEFSS + PDVRTV +DP+ + +PV ++ R TA PKFT F +F S
Sbjct: 448 GTTLFGLAKEFSSITPPDVRTVAVDPSALMLPWSPVAEMVRPTASGPKFTIFTLRKLFSS 269
Score = 54.9 bits (114), Expect(2) = 6e-027
Identities = 31/68 (45%), Positives = 38/68 (55%)
Frame = +3 / +2
Query: 75 DGKLCSTPCKRQ*PKVEPRCYSL*LMAALWMSKLLDAALFQRFKQRHTSSVHYSTTELRP 254
DGKLC+ KR P VEPR Y L L+AA+ S+LLDA F R K+ + S L
Sbjct: 59 DGKLCTALHKR*RPHVEPRRYRLQLLAAIRNSQLLDADAFPRIKRCYGPSHQSQFKLLWF 238
Query: 255 TGHICYHL 278
G IC H+
Sbjct: 239 AGSICDHV 262
Score = 35.3 bits (71), Expect(2) = 1e-032
Identities = 18/59 (30%), Positives = 29/59 (49%)
Frame = +3 / +2
Query: 297 EHLHENKGCKFWKQSC*SEYICNWVGN*YSDIWIHKDGSHVGLAAG*ELLSTARQGGAG 473
E L E++ C+F + ++ +W + IH+D SH+ G EL +QG AG
Sbjct: 275 EQLPESEDCEFRARCRGPHHLGDWAPGQHQCARIHRDRSHIWRCNGRELFR*PKQGCAG 451
>gb|BJ466672.1|BJ466672 BJ466672 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags33f17 3', mRNA sequence
Length = 715
Score = 125 bits (270), Expect = 1e-028
Identities = 51/100 (51%), Positives = 66/100 (66%)
Frame = +1 / -3
Query: 61 SDVVEMASCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYWMLHFFKDSSSATLHPSTIQL 240
SD+V MAS APL VNDNDQ WNPDAIVFNSWQ YG P+YWM FF++SS A +HP TI
Sbjct: 704 SDIVHMASYAPLFVNDNDQTWNPDAIVFNSWQQYGTPSYWMQKFFRESSGAMIHPITISS 525
Query: 241 PNYDQLVTSAITWNNPHDGNTYMKIKVVNFGSKAVSLNIS 360
L SAITW + + +KI + ++++++S
Sbjct: 524 SYSGSLAASAITWQDSGNNFLRVKIVNFGSDTVSLTISVS 405
Score = 96.3 bits (205), Expect(2) = 2e-032
Identities = 41/75 (54%), Positives = 52/75 (69%)
Frame = +1 / -3
Query: 295 GNTYMKIKVVNFGSKAVSLNISVTGLETDIQTFGSIKTVLTSGWLRDENSFQQPDKVVPA 474
GN ++++K+VNFGS VSL ISV+GL+ I GS TVLTS ++DENSF P KVVP
Sbjct: 476 GNNFLRVKIVNFGSDTVSLTISVSGLQASINALGSNATVLTSSNVKDENSFSNPTKVVPV 297
Query: 475 ASPITNAGKQMGVIL 519
S + NA +QM V L
Sbjct: 296 TSQLHNAAEQMQVTL 252
Score = 75.4 bits (159), Expect(2) = 3e-028
Identities = 39/58 (67%), Positives = 43/58 (74%)
Frame = -3 / +1
Query: 233 IVDG*SVALLESLKKCSIQ*FGHP*CCHELKTIASGFHLWSLSFTRSGAQLAISTTSL 60
IV G* +A L+S K IQ* G P CC ELKTIASGFH+ SLSFT+SGA LAI T SL
Sbjct: 532 IVIG*IMAPLDSRKNFCIQ*LGVPYCCQELKTIASGFHV*SLSFTKSGA*LAI*TISL 705
Score = 68.1 bits (143), Expect(2) = 3e-028
Identities = 36/75 (48%), Positives = 44/75 (58%)
Frame = -3 / +1
Query: 518 NITPICLPALVIGLAAGTTLSGC*KEFSSRSQPDVRTVFMDPNV*ISVSNPVTDIFRLTA 339
++T IC AL L GTTL G KEFSS + DVRTV DP+ + +P T+I RLT
Sbjct: 253 SVTCICSAALCN*LVTGTTLVGLLKEFSSFTLLDVRTVAFDPSALMLAWSPDTEIVRLTV 432
Query: 338 LLPKFTTFIFM*VFP 294
PKFT F +FP
Sbjct: 433 SDPKFTIFTLRKLFP 477
Score = 62.2 bits (130), Expect(2) = 2e-032
Identities = 32/67 (47%), Positives = 38/67 (56%)
Frame = +3 / -2
Query: 78 GKLCSTPCKRQ*PKVEPRCYSL*LMAALWMSKLLDAALFQRFKQRHTSSVHYSTTELRPT 257
GKLC+ CKRQ VE RCY L L+A + S LLDA +F R H SS + L +
Sbjct: 687 GKLCTALCKRQRSNVESRCYRLQLLATIRNS*LLDAEVFPRI*WCHDSSNYNQFQLLWFS 508
Query: 258 GHICYHL 278
ICYHL
Sbjct: 507 SSICYHL 487
>gb|BJ455660.1|BJ455660 BJ455660 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak17l17 3', mRNA sequence
Length = 704
Score = 129 bits (278), Expect = 1e-029
Identities = 52/102 (50%), Positives = 68/102 (66%)
Frame = +1 / -3
Query: 55 RNSDVVEMASCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYWMLHFFKDSSSATLHPSTI 234
+NSD+V MAS APL VNDNDQ WNPDAIVFNSWQ YG P+YWM FF++SS A +HP TI
Sbjct: 684 KNSDIVHMASYAPLFVNDNDQTWNPDAIVFNSWQQYGTPSYWMQKFFRESSGAMIHPITI 505
Query: 235 QLPNYDQLVTSAITWNNPHDGNTYMKIKVVNFGSKAVSLNIS 360
L SAITW + + +KI + ++++++S
Sbjct: 504 SSSYSGSLAASAITWQDSGNNFLRVKIVNFGSDTVSLTISVS 379
Score = 96.3 bits (205), Expect(2) = 2e-032
Identities = 41/75 (54%), Positives = 52/75 (69%)
Frame = +1 / -3
Query: 295 GNTYMKIKVVNFGSKAVSLNISVTGLETDIQTFGSIKTVLTSGWLRDENSFQQPDKVVPA 474
GN ++++K+VNFGS VSL ISV+GL+ I GS TVLTS ++DENSF P KVVP
Sbjct: 450 GNNFLRVKIVNFGSDTVSLTISVSGLQASINALGSNATVLTSSNVKDENSFSNPTKVVPV 271
Query: 475 ASPITNAGKQMGVIL 519
S + NA +QM V L
Sbjct: 270 TSQLHNAAEQMQVTL 226
Score = 86.3 bits (183), Expect(2) = 2e-031
Identities = 43/65 (66%), Positives = 49/65 (75%)
Frame = -3 / +2
Query: 233 IVDG*SVALLESLKKCSIQ*FGHP*CCHELKTIASGFHLWSLSFTRSGAQLAISTTSLFL 54
IV G* +A L+S K IQ* G P CC ELKTIASGFH+ SLSFT+SGA LAI T SLF
Sbjct: 506 IVIG*IMAPLDSRKNFCIQ*LGVPYCCQELKTIASGFHV*SLSFTKSGA*LAI*TISLFF 685
Query: 53 SNPMR 39
S+P+R
Sbjct: 686 SSPVR 700
Score = 68.1 bits (143), Expect(2) = 2e-031
Identities = 36/75 (48%), Positives = 44/75 (58%)
Frame = -3 / +2
Query: 518 NITPICLPALVIGLAAGTTLSGC*KEFSSRSQPDVRTVFMDPNV*ISVSNPVTDIFRLTA 339
++T IC AL L GTTL G KEFSS + DVRTV DP+ + +P T+I RLT
Sbjct: 227 SVTCICSAALCN*LVTGTTLVGLLKEFSSFTLLDVRTVAFDPSALMLAWSPDTEIVRLTV 406
Query: 338 LLPKFTTFIFM*VFP 294
PKFT F +FP
Sbjct: 407 SDPKFTIFTLRKLFP 451
Score = 62.2 bits (130), Expect(2) = 2e-032
Identities = 32/67 (47%), Positives = 38/67 (56%)
Frame = +3 / -2
Query: 78 GKLCSTPCKRQ*PKVEPRCYSL*LMAALWMSKLLDAALFQRFKQRHTSSVHYSTTELRPT 257
GKLC+ CKRQ VE RCY L L+A + S LLDA +F R H SS + L +
Sbjct: 661 GKLCTALCKRQRSNVESRCYRLQLLATIRNS*LLDAEVFPRI*WCHDSSNYNQFQLLWFS 482
Query: 258 GHICYHL 278
ICYHL
Sbjct: 481 SSICYHL 461
>gb|BU981962.1|BU981962 HA25B23r HA Hordeum vulgare subsp. vulgare cDNA clone HA25B23
5-PRIME, mRNA sequence
Length = 558
Score = 81.8 bits (173), Expect(2) = 2e-032
Identities = 31/59 (52%), Positives = 37/59 (62%)
Frame = +1 / +1
Query: 118 RWNPDAIVFNSWQHYGCPNYWMLHFFKDSSSATLHPSTIQLPNYDQLVTSAITWNNPHD 294
R PDAIVFNSWQ YG P+YWM FF++SS A +HP + L SAITW + D
Sbjct: 1 RTRPDAIVFNSWQQYGTPSYWMQTFFRESSGAVIHPMRLNSSYSGSLAASAITWGDNED 177
Score = 76.7 bits (162), Expect(2) = 2e-032
Identities = 33/72 (45%), Positives = 47/72 (65%)
Frame = +1 / +1
Query: 304 YMKIKVVNFGSKAVSLNISVTGLETDIQTFGSIKTVLTSGWLRDENSFQQPDKVVPAASP 483
+++IK+VNFGS AV+L +S GL+ + T S TVLTS + DEN+F P VVP S
Sbjct: 181 FLRIKIVNFGSNAVNLTLSAIGLQAGVNTSRSAVTVLTSDNVLDENTFDDPRNVVPERSD 360
Query: 484 ITNAGKQMGVIL 519
+ NA ++M +L
Sbjct: 361 LPNAAEEMQAML 396
>gb|BJ464212.1|BJ464212 BJ464212 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags33f17 5', mRNA sequence
Length = 591
Score = 128 bits (275), Expect = 3e-029
Identities = 51/95 (53%), Positives = 59/95 (62%)
Frame = +1 / +1
Query: 1 RGTXXXXXXXXXXXXXXXRNSDVVEMASCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYW 180
RG+ +NSD+V MAS APL VNDNDQ WNPDAIVFNSWQ YG P+YW
Sbjct: 283 RGSLLGSLAEAAFLTGLEKNSDIVHMASYAPLFVNDNDQTWNPDAIVFNSWQQYGTPSYW 462
Query: 181 MLHFFKDSSSATLHPSTIQLPNYDQLVTSAITWNN 285
M FF++SS A +HP TI L SAITW +
Sbjct: 463 MQKFFRESSGAMIHPITISSSYSGSLAASAITWQD 567
>gb|BF259719.2|BF259719 HVSMEf0020B07f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0020B07f, mRNA sequence
Length = 832
Score = 127 bits (274), Expect = 4e-029
Identities = 51/93 (54%), Positives = 58/93 (62%)
Frame = +1 / +2
Query: 1 RGTXXXXXXXXXXXXXXXRNSDVVEMASCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYW 180
RG+ +NSD+V MAS APL VNDNDQ WNPDAIVFNSWQ YG P+YW
Sbjct: 251 RGSLLGSLAEAAFLTGLEKNSDIVHMASYAPLFVNDNDQTWNPDAIVFNSWQQYGTPSYW 430
Query: 181 MLHFFKDSSSATLHPSTIQLPNYDQLVTSAITW 279
M FF++S A +HP TI L TSAITW
Sbjct: 431 MQKFFRESRGAMIHPITISSSYSGSLATSAITW 529
>gb|AV917599.1|AV917599 AV917599 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags10j19 3', mRNA sequence
Length = 656
Score = 104 bits (223), Expect = 4e-022
Identities = 41/86 (47%), Positives = 55/86 (63%)
Frame = +1 / -2
Query: 103 NDNDQRWNPDAIVFNSWQHYGCPNYWMLHFFKDSSSATLHPSTIQLPNYDQLVTSAITWN 282
NDNDQ WNPDAIVFNSWQ YG P+YWM FF++SS A +HP TI L SAITW
Sbjct: 655 NDNDQTWNPDAIVFNSWQQYGTPSYWMQKFFRESSGAMIHPITISSSYSGSLAASAITWQ 476
Query: 283 NPHDGNTYMKIKVVNFGSKAVSLNIS 360
+ + +KI + ++++++S
Sbjct: 475 DSGNNFLRVKIVNFGSDTVSLTISVS 398
Score = 96.3 bits (205), Expect(2) = 3e-028
Identities = 41/75 (54%), Positives = 52/75 (69%)
Frame = +1 / -2
Query: 295 GNTYMKIKVVNFGSKAVSLNISVTGLETDIQTFGSIKTVLTSGWLRDENSFQQPDKVVPA 474
GN ++++K+VNFGS VSL ISV+GL+ I GS TVLTS ++DENSF P KVVP
Sbjct: 469 GNNFLRVKIVNFGSDTVSLTISVSGLQASINALGSNATVLTSSNVKDENSFSNPTKVVPV 290
Query: 475 ASPITNAGKQMGVIL 519
S + NA +QM V L
Sbjct: 289 TSQLHNAAEQMQVTL 245
Score = 68.1 bits (143), Expect(2) = 4e-023
Identities = 36/75 (48%), Positives = 44/75 (58%)
Frame = -3 / +3
Query: 518 NITPICLPALVIGLAAGTTLSGC*KEFSSRSQPDVRTVFMDPNV*ISVSNPVTDIFRLTA 339
++T IC AL L GTTL G KEFSS + DVRTV DP+ + +P T+I RLT
Sbjct: 246 SVTCICSAALCN*LVTGTTLVGLLKEFSSFTLLDVRTVAFDPSALMLAWSPDTEIVRLTV 425
Query: 338 LLPKFTTFIFM*VFP 294
PKFT F +FP
Sbjct: 426 SDPKFTIFTLRKLFP 470
Score = 58.5 bits (122), Expect(2) = 4e-023
Identities = 29/44 (65%), Positives = 32/44 (72%)
Frame = -3 / +3
Query: 233 IVDG*SVALLESLKKCSIQ*FGHP*CCHELKTIASGFHLWSLSF 102
IV G* +A L+S K IQ* G P CC ELKTIASGFH+ SLSF
Sbjct: 525 IVIG*IMAPLDSRKNFCIQ*LGVPYCCQELKTIASGFHV*SLSF 656
Score = 48.0 bits (99), Expect(2) = 3e-028
Identities = 27/59 (45%), Positives = 32/59 (54%)
Frame = +3 / -1
Query: 102 KRQ*PKVEPRCYSL*LMAALWMSKLLDAALFQRFKQRHTSSVHYSTTELRPTGHICYHL 278
KRQ VE RCY L L+A + S LLDA +F R H SS + L + ICYHL
Sbjct: 656 KRQRSNVESRCYRLQLLATIRNS*LLDAEVFPRI*WCHDSSNYNQFQLLWFSSSICYHL 480
>gb|AV930332.1|AV930332 AV930332 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd22b16 3', mRNA sequence
Length = 646
Score = 104 bits (223), Expect = 4e-022
Identities = 41/86 (47%), Positives = 55/86 (63%)
Frame = +1 / -2
Query: 103 NDNDQRWNPDAIVFNSWQHYGCPNYWMLHFFKDSSSATLHPSTIQLPNYDQLVTSAITWN 282
NDNDQ WNPDAIVFNSWQ YG P+YWM FF++SS A +HP TI L SAITW
Sbjct: 645 NDNDQTWNPDAIVFNSWQQYGTPSYWMQKFFRESSGAMIHPITISSSYSGSLAASAITWQ 466
Query: 283 NPHDGNTYMKIKVVNFGSKAVSLNIS 360
+ + +KI + ++++++S
Sbjct: 465 DSGNNFLRVKIVNFGSDTVSLTISVS 388
Score = 96.3 bits (205), Expect(2) = 3e-028
Identities = 41/75 (54%), Positives = 52/75 (69%)
Frame = +1 / -2
Query: 295 GNTYMKIKVVNFGSKAVSLNISVTGLETDIQTFGSIKTVLTSGWLRDENSFQQPDKVVPA 474
GN ++++K+VNFGS VSL ISV+GL+ I GS TVLTS ++DENSF P KVVP
Sbjct: 459 GNNFLRVKIVNFGSDTVSLTISVSGLQASINALGSNATVLTSSNVKDENSFSNPTKVVPV 280
Query: 475 ASPITNAGKQMGVIL 519
S + NA +QM V L
Sbjct: 279 TSQLHNAAEQMQVTL 235
Score = 68.1 bits (143), Expect(2) = 4e-023
Identities = 36/75 (48%), Positives = 44/75 (58%)
Frame = -3 / +2
Query: 518 NITPICLPALVIGLAAGTTLSGC*KEFSSRSQPDVRTVFMDPNV*ISVSNPVTDIFRLTA 339
++T IC AL L GTTL G KEFSS + DVRTV DP+ + +P T+I RLT
Sbjct: 236 SVTCICSAALCN*LVTGTTLVGLLKEFSSFTLLDVRTVAFDPSALMLAWSPDTEIVRLTV 415
Query: 338 LLPKFTTFIFM*VFP 294
PKFT F +FP
Sbjct: 416 SDPKFTIFTLRKLFP 460
Score = 58.5 bits (122), Expect(2) = 4e-023
Identities = 29/44 (65%), Positives = 32/44 (72%)
Frame = -3 / +2
Query: 233 IVDG*SVALLESLKKCSIQ*FGHP*CCHELKTIASGFHLWSLSF 102
IV G* +A L+S K IQ* G P CC ELKTIASGFH+ SLSF
Sbjct: 515 IVIG*IMAPLDSRKNFCIQ*LGVPYCCQELKTIASGFHV*SLSF 646
Score = 48.0 bits (99), Expect(2) = 3e-028
Identities = 27/59 (45%), Positives = 32/59 (54%)
Frame = +3 / -1
Query: 102 KRQ*PKVEPRCYSL*LMAALWMSKLLDAALFQRFKQRHTSSVHYSTTELRPTGHICYHL 278
KRQ VE RCY L L+A + S LLDA +F R H SS + L + ICYHL
Sbjct: 646 KRQRSNVESRCYRLQLLATIRNS*LLDAEVFPRI*WCHDSSNYNQFQLLWFSSSICYHL 470
>gb|AV927973.1|AV927973 AV927973 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd13h16 3', mRNA sequence
Length = 642
Score = 104 bits (223), Expect = 4e-022
Identities = 41/86 (47%), Positives = 55/86 (63%)
Frame = +1 / -2
Query: 103 NDNDQRWNPDAIVFNSWQHYGCPNYWMLHFFKDSSSATLHPSTIQLPNYDQLVTSAITWN 282
NDNDQ WNPDAIVFNSWQ YG P+YWM FF++SS A +HP TI L SAITW
Sbjct: 641 NDNDQTWNPDAIVFNSWQQYGTPSYWMQKFFRESSGAMIHPITISSSYSGSLAASAITWQ 462
Query: 283 NPHDGNTYMKIKVVNFGSKAVSLNIS 360
+ + +KI + ++++++S
Sbjct: 461 DSGNNFLRVKIVNFGSDTVSLTISVS 384
Score = 96.3 bits (205), Expect(2) = 3e-028
Identities = 41/75 (54%), Positives = 52/75 (69%)
Frame = +1 / -2
Query: 295 GNTYMKIKVVNFGSKAVSLNISVTGLETDIQTFGSIKTVLTSGWLRDENSFQQPDKVVPA 474
GN ++++K+VNFGS VSL ISV+GL+ I GS TVLTS ++DENSF P KVVP
Sbjct: 455 GNNFLRVKIVNFGSDTVSLTISVSGLQASINALGSNATVLTSSNVKDENSFSNPTKVVPV 276
Query: 475 ASPITNAGKQMGVIL 519
S + NA +QM V L
Sbjct: 275 TSQLHNAAEQMQVTL 231
Score = 68.1 bits (143), Expect(2) = 4e-023
Identities = 36/75 (48%), Positives = 44/75 (58%)
Frame = -3 / +1
Query: 518 NITPICLPALVIGLAAGTTLSGC*KEFSSRSQPDVRTVFMDPNV*ISVSNPVTDIFRLTA 339
++T IC AL L GTTL G KEFSS + DVRTV DP+ + +P T+I RLT
Sbjct: 232 SVTCICSAALCN*LVTGTTLVGLLKEFSSFTLLDVRTVAFDPSALMLAWSPDTEIVRLTV 411
Query: 338 LLPKFTTFIFM*VFP 294
PKFT F +FP
Sbjct: 412 SDPKFTIFTLRKLFP 456
Score = 58.5 bits (122), Expect(2) = 4e-023
Identities = 29/44 (65%), Positives = 32/44 (72%)
Frame = -3 / +1
Query: 233 IVDG*SVALLESLKKCSIQ*FGHP*CCHELKTIASGFHLWSLSF 102
IV G* +A L+S K IQ* G P CC ELKTIASGFH+ SLSF
Sbjct: 511 IVIG*IMAPLDSRKNFCIQ*LGVPYCCQELKTIASGFHV*SLSF 642
Score = 48.0 bits (99), Expect(2) = 3e-028
Identities = 27/59 (45%), Positives = 32/59 (54%)
Frame = +3 / -1
Query: 102 KRQ*PKVEPRCYSL*LMAALWMSKLLDAALFQRFKQRHTSSVHYSTTELRPTGHICYHL 278
KRQ VE RCY L L+A + S LLDA +F R H SS + L + ICYHL
Sbjct: 642 KRQRSNVESRCYRLQLLATIRNS*LLDAEVFPRI*WCHDSSNYNQFQLLWFSSSICYHL 466
>gb|BU988298.1|BU988298 HF17D19r HF Hordeum vulgare subsp. vulgare cDNA clone HF17D19
5-PRIME, mRNA sequence
Length = 638
Score = 122 bits (263), Expect = 1e-027
Identities = 50/92 (54%), Positives = 57/92 (61%)
Frame = +1 / +1
Query: 1 RGTXXXXXXXXXXXXXXXRNSDVVEMASCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYW 180
RG+ +NSD+V MAS APL VNDNDQ WNPDAIVFNSWQ YG P+YW
Sbjct: 361 RGSLLGSLAEAAFLTGLEKNSDIVHMASYAPLFVNDNDQTWNPDAIVFNSWQQYGTPSYW 540
Query: 181 MLHFFKDSSSATLHPSTIQLPNYDQLVTSAIT 276
M FF++SS A +HP TI L SAIT
Sbjct: 541 MQKFFRESSGAMIHPITISSSYSGSLAASAIT 636
>gb|AV934949.1|AV934949 AV934949 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal2e14 3', mRNA sequence
Length = 654
Score = 104 bits (223), Expect = 4e-022
Identities = 41/86 (47%), Positives = 55/86 (63%)
Frame = +1 / -1
Query: 103 NDNDQRWNPDAIVFNSWQHYGCPNYWMLHFFKDSSSATLHPSTIQLPNYDQLVTSAITWN 282
NDNDQ WNPDAIVFNSWQ YG P+YWM FF++SS A +HP TI L SAITW
Sbjct: 654 NDNDQTWNPDAIVFNSWQQYGTPSYWMQKFFRESSGAMIHPITISSSYSGSLAASAITWQ 475
Query: 283 NPHDGNTYMKIKVVNFGSKAVSLNIS 360
+ + +KI + ++++++S
Sbjct: 474 DSGNNFLRVKIVNFGSDTVSLTISVS 397
Score = 96.3 bits (205), Expect(2) = 1e-027
Identities = 41/75 (54%), Positives = 52/75 (69%)
Frame = +1 / -1
Query: 295 GNTYMKIKVVNFGSKAVSLNISVTGLETDIQTFGSIKTVLTSGWLRDENSFQQPDKVVPA 474
GN ++++K+VNFGS VSL ISV+GL+ I GS TVLTS ++DENSF P KVVP
Sbjct: 468 GNNFLRVKIVNFGSDTVSLTISVSGLQASINALGSNATVLTSSNVKDENSFSNPTKVVPV 289
Query: 475 ASPITNAGKQMGVIL 519
S + NA +QM V L
Sbjct: 288 TSQLHNAAEQMQVTL 244
Score = 68.1 bits (143), Expect(2) = 2e-022
Identities = 36/75 (48%), Positives = 44/75 (58%)
Frame = -3 / +2
Query: 518 NITPICLPALVIGLAAGTTLSGC*KEFSSRSQPDVRTVFMDPNV*ISVSNPVTDIFRLTA 339
++T IC AL L GTTL G KEFSS + DVRTV DP+ + +P T+I RLT
Sbjct: 245 SVTCICSAALCN*LVTGTTLVGLLKEFSSFTLLDVRTVAFDPSALMLAWSPDTEIVRLTV 424
Query: 338 LLPKFTTFIFM*VFP 294
PKFT F +FP
Sbjct: 425 SDPKFTIFTLRKLFP 469
Score = 55.8 bits (116), Expect(2) = 2e-022
Identities = 28/43 (65%), Positives = 31/43 (72%)
Frame = -3 / +2
Query: 233 IVDG*SVALLESLKKCSIQ*FGHP*CCHELKTIASGFHLWSLS 105
IV G* +A L+S K IQ* G P CC ELKTIASGFH+ SLS
Sbjct: 524 IVIG*IMAPLDSRKNFCIQ*LGVPYCCQELKTIASGFHV*SLS 652
Score = 45.8 bits (94), Expect(2) = 1e-027
Identities = 26/58 (44%), Positives = 31/58 (53%)
Frame = +3 / -3
Query: 105 RQ*PKVEPRCYSL*LMAALWMSKLLDAALFQRFKQRHTSSVHYSTTELRPTGHICYHL 278
RQ VE RCY L L+A + S LLDA +F R H SS + L + ICYHL
Sbjct: 652 RQRSNVESRCYRLQLLATIRNS*LLDAEVFPRI*WCHDSSNYNQFQLLWFSSSICYHL 479
>gb|CA024671.1|CA024671 HZ49P14r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ49P14
5-PRIME, mRNA sequence
Length = 597
Score = 112 bits (240), Expect(2) = 9e-027
Identities = 43/73 (58%), Positives = 52/73 (71%)
Frame = +1 / +1
Query: 58 NSDVVEMASCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYWMLHFFKDSSSATLHPSTIQ 237
NSD+V MAS APL VN+ND++W PDAIVFNSWQ YG P+YWM FF++SS A +HP +
Sbjct: 379 NSDIVHMASYAPLFVNENDRQWTPDAIVFNSWQQYGTPSYWMQTFFRESSGAVIHPMRLN 558
Query: 238 LPNYDQLVTSAIT 276
L SAIT
Sbjct: 559 SSYSGSLAASAIT 597
Score = 27.1 bits (53), Expect(2) = 9e-027
Identities = 10/18 (55%), Positives = 11/18 (61%)
Frame = +3 / +3
Query: 6 NTNSCFS*SCISHWIRKE 59
N +S SCI HWIR E
Sbjct: 327 NPSSFIGRSCIPHWIRSE 380
>gb|CA028812.1|CA028812 HZ63F05r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ63F05
5-PRIME, mRNA sequence
Length = 415
Score = 106 bits (227), Expect(2) = 4e-025
Identities = 38/56 (67%), Positives = 46/56 (82%)
Frame = +1 / +1
Query: 58 NSDVVEMASCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYWMLHFFKDSSSATLHP 225
NSD+V MAS APL VN+ND++W PDAIVFNSWQ YG P+YWM FF++SS A +HP
Sbjct: 205 NSDIVHMASYAPLFVNENDRQWTPDAIVFNSWQQYGTPSYWMQTFFRESSGAVIHP 372
Score = 27.1 bits (53), Expect(2) = 4e-025
Identities = 10/18 (55%), Positives = 11/18 (61%)
Frame = +3 / +3
Query: 6 NTNSCFS*SCISHWIRKE 59
N +S SCI HWIR E
Sbjct: 153 NPSSFIGRSCIPHWIRSE 206
>gb|AV925177.1|AV925177 AV925177 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd22b16 5', mRNA sequence
Length = 639
Score = 110 bits (236), Expect = 6e-024
Identities = 42/74 (56%), Positives = 49/74 (66%)
Frame = +1 / +1
Query: 1 RGTXXXXXXXXXXXXXXXRNSDVVEMASCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYW 180
RG+ +NSD+V MAS APL VNDNDQ WNPDAIVFNSWQ YG P+YW
Sbjct: 418 RGSLLGSLAEAAFLTGLEKNSDIVHMASYAPLFVNDNDQTWNPDAIVFNSWQQYGTPSYW 597
Query: 181 MLHFFKDSSSATLH 222
M FF++SS A +H
Sbjct: 598 MQKFFRESSGAMIH 639
>gb|BF267850.2|BF267850 HV_CEa0019B08f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0019B08f, mRNA sequence
Length = 769
Score = 90.9 bits (193), Expect(2) = 3e-023
Identities = 38/95 (40%), Positives = 49/95 (51%)
Frame = +1 / +2
Query: 1 RGTXXXXXXXXXXXXXXXRNSDVVEMASCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYW 180
RG+ +NSD+V M S APL + DQ WN DAI N WQ YG P+ W
Sbjct: 251 RGSLLGSLAEADFLTGLDKNSDIVHMGSHAPLFGDHIDQTWNTDAIRLNYWQRYGTPSTW 430
Query: 181 MLHFFKDSSSATLHPSTIQLPNYDQLVTSAITWNN 285
M F ++SS A +HP TI + AITW++
Sbjct: 431 MRRFLRESSGAMIHPITISFSYSGAVAAFAITWHD 535
Score = 36.7 bits (74), Expect(2) = 3e-023
Identities = 17/34 (50%), Positives = 20/34 (58%)
Frame = +1 / +1
Query: 316 KVVNFGSKAVSLNISVTGLETDIQTFGSIKTVLT 417
K+ NF S VSL +S GL+ I GS TVLT
Sbjct: 559 KIGNFESGTVSLTVSACGLQATISALGSHATVLT 660
>gb|BF631424.2|BF631424 HVSMEb0015O10f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0015O10f, mRNA sequence
Length = 762
Score = 101 bits (217), Expect = 2e-021
Identities = 38/73 (52%), Positives = 48/73 (65%)
Frame = +1 / +2
Query: 100 VNDNDQRWNPDAIVFNSWQHYGCPNYWMLHFFKDSSSATLHPSTIQLPNYDQLVTSAITW 279
VNDNDQ WNPDA+VFNSWQ YG P+YWM FF++SS A +HP I L SA+TW
Sbjct: 89 VNDNDQTWNPDAVVFNSWQQYGTPSYWMQKFFRESSGAMIHPIAISSSYSGSLTASAVTW 268
Query: 280 NNPHDGNTYMKIK 318
+ + +KI+
Sbjct: 269 QDSGNN*LRVKIE 307
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.316 0.130 0.405
Matrix: BLOSUM62
Number of Hits to DB: 280,646,458
Number of Sequences: 312970
Number of extensions: 4458067
Number of successful extensions: 80783
Number of sequences better than 1.0e-020: 29
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 5298480196
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.6 bits)