TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF41c07.yg.2.1
(691 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY177665.1| Hordeum vulgare subsp. vulgare putative call... 212 e-117
gb|AV923029.1|AV923029 AV923029 K. Sato unpublished cDNA li... 189 4e-067
gb|BJ462458.1|BJ462458 BJ462458 K. Sato unpublished cDNA li... 185 9e-050
gb|BU969103.1|BU969103 HB10J09r BC Hordeum vulgare subsp. v... 137 6e-032
gb|AV836635.1|AV836635 AV836635 K. Sato unpublished cDNA li... 121 5e-027
gb|AV934137.1|AV934137 AV934137 K. Sato unpublished cDNA li... 120 1e-026
gb|BJ470174.1|BJ470174 BJ470174 K. Sato unpublished cDNA li... 120 1e-026
>gb|AY177665.1| Hordeum vulgare subsp. vulgare putative callose synthase mRNA,
complete cds
Length = 6180
Score = 212 bits (457), Expect(3) = e-117
Identities = 86/89 (96%), Positives = 89/89 (100%)
Frame = -1 / +3
Query: 268 LKVRMHYGHPDVFDRIFHITRGGISKASRVINISEDIYAGFNSTLRQGNITHHEYIQVGK 89
LKVRMHYGHPDVFDRIFHITRGGISKASR+INISEDI+AGFNSTLRQGNITHHEYIQVGK
Sbjct: 4230 LKVRMHYGHPDVFDRIFHITRGGISKASRIINISEDIFAGFNSTLRQGNITHHEYIQVGK 4409
Query: 88 GRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
GRDVGLNQIALFEGKVAGGNGEQVLSRD+
Sbjct: 4410 GRDVGLNQIALFEGKVAGGNGEQVLSRDI 4496
Score = 163 bits (351), Expect(3) = e-117
Identities = 64/79 (81%), Positives = 70/79 (88%)
Frame = -1 / +3
Query: 637 EYXSKLVXXDVHGKDQEIYCIKLPGNPKLGEGXPENQNHAIIFTRGDAVQTIDMNQDNYL 458
EY SKLV D+HGKD+EIY +KLPGNPKLGEG PENQNHA+IFTRG+AVQTIDMNQDNY
Sbjct: 3867 EYYSKLVKADIHGKDKEIYSVKLPGNPKLGEGKPENQNHAVIFTRGNAVQTIDMNQDNYF 4046
Query: 457 EEAMKMRNLLEEFHNAHGK 401
EEA+KMRNLLEEF HGK
Sbjct: 4047 EEALKMRNLLEEFSQNHGK 4103
Score = 152 bits (326), Expect(3) = 9e-074
Identities = 67/86 (77%), Positives = 70/86 (81%)
Frame = +3 / -3
Query: 6 SRLRTCSPLPPATFPSNSAIWFKPTSRPFPTWIYSWCVMFPWRKVELNPAYISSLILITR 185
S LRTCSP PPATFPSNSAI PTS P PTWIYSW VMFP R+VELNPA ISSLIL+ R
Sbjct: 4492 SLLRTCSPFPPATFPSNSAI*LSPTSLPLPTWIYSW*VMFP*RRVELNPANISSLILMIR 4313
Query: 186 DALLMPPRVM*NIRSKTSGCP*CIRT 263
DALLMPP V+*NI S TSG P*CI T
Sbjct: 4312 DALLMPPLVI*NILSNTSG*P*CILT 4235
Score = 99.6 bits (211), Expect(3) = 5e-033
Identities = 43/89 (48%), Positives = 64/89 (71%)
Frame = +2 / -2
Query: 2 HISAENLLTIASSNFPLK*CNLVQANITPFPHLDIFMVCDVPLAQSRIEPGIYIFAYINH 181
+ISA+NL ++S NF K*C+L++ NI+ F +LDI MV VPL QSRI+P YI I+
Sbjct: 4496 YISAKNLFAVSSRNFSFK*CDLIKPNISSFTNLDILMVSYVPLTQSRIKPCKYILTDIDD 4317
Query: 182 SRRLANATSGYVKYPIKNIRMSIVHSNFE 268
+ RL +AT +KY IK++ ++I+HS+F+
Sbjct: 4316 TGRLTDATPRNMKYSIKHVWVTIMHSHFQ 4230
Score = 87.2 bits (184), Expect(3) = e-117
Identities = 38/43 (88%), Positives = 40/43 (93%)
Frame = -1 / +3
Query: 400 HGIRKPTILGVREHVFTGSVSSLASFMSKQETSFVTLGQRVLA 272
HG KP+ILGVREHVFTGSVSSLASFMS QETSFVTLGQRVL+
Sbjct: 4095 HGKFKPSILGVREHVFTGSVSSLASFMSNQETSFVTLGQRVLS 4223
Score = 84.0 bits (177), Expect(3) = 9e-074
Identities = 36/78 (46%), Positives = 50/78 (64%)
Frame = +2 / -2
Query: 404 SMSIMKFFK*ISHFHCFLQVVILIHIDRLNCISSSEDYGMVLVLRXAFTXLGVPRQFNAV 583
+M + +F K ISHF C L++V+LIHI+ L IS+SE Y MVL+ L + RQFN +
Sbjct: 4100 AMILREFLKQISHFECLLEIVVLIHINGLYSISASEYYSMVLIFGFTLPKLWIARQFNRI 3921
Query: 584 YFLVFAMNIXLHXLGXIL 637
FLV +M++ H L IL
Sbjct: 3920 NFLVLSMDVSFHKL*VIL 3867
Score = 79.9 bits (168), Expect(3) = 9e-074
Identities = 33/43 (76%), Positives = 35/43 (81%)
Frame = +3 / -3
Query: 276 RTRCPKVTKLVSCFDINDASEETLPVNTCSLTPRIVGFRMPCF 404
RTRCP VTKLVS FDIN+A EETLPV TCSLTPRI G +P F
Sbjct: 4219 RTRCPNVTKLVS*FDINEAREETLPVKTCSLTPRIEGLNLP*F 4091
Score = 73.5 bits (154), Expect(3) = 3e-034
Identities = 39/84 (46%), Positives = 50/84 (59%)
Frame = -3 / +2
Query: 266 QSSNALWTS*CF*SDISHNPRWH*QGVSSD*YKRRYICRVQFDFAPREHHTP*IYPGGER 87
+S NALW+ * +IS+ WH GV Y+ YICRV F A REH++P*+YPG R
Sbjct: 4232 ESENALWSPRRV**NISYYEGWHQ*GVPYHQYQ*GYICRV*FYSASREHNSP*VYPGW*R 4411
Query: 86 A*CWLEPNCTI*GESCWRQW*ASS 15
CW + TI* +SC R+ SS
Sbjct: 4412 KRCWA*SDRTI*RKSCGRKRRTSS 4483
Score = 62.0 bits (129), Expect(3) = 3e-034
Identities = 32/79 (40%), Positives = 44/79 (55%)
Frame = -3 / +2
Query: 638 RVXXQAXXX*CSWQRPRNILH*IAWEPQAX*RXT*EPKPCHNLHSRRCSSDDRYESG*LL 459
RV +A * W+ N+ *IAW+ +A T + KPC N+HSR+C ++ YESG L
Sbjct: 3866 RVLLKACES*HPWKGQGNLFC*IAWQSKAWGG*TRKSKPCCNIHSRKCCTNH*YESGQLF 4045
Query: 458 GGSNENEKFT*RIS*CSWK 402
G +N K * I SW+
Sbjct: 4046 RGGTQNAKSA*GILSKSWQ 4102
Score = 47.8 bits (98), Expect(3) = 5e-033
Identities = 25/34 (73%), Positives = 27/34 (79%)
Frame = +3 / -3
Query: 420 NSSSKFLIFIASSK*LS*FISIV*TASPRVKIMA 521
NSSS+F I ASSK*LS*FIS+V TA PRV I A
Sbjct: 4084 NSSSRFRILSASSK*LS*FISMVCTAFPRVNITA 3983
Score = 47.3 bits (97), Expect(3) = 3e-034
Identities = 17/43 (39%), Positives = 24/43 (55%)
Frame = -3 / +2
Query: 404 KTWHTEAHNPRCEGACIHGECLFAGVIYVETGD*FCYFGATCS 276
K+W +A N C C+H +C IYV++G+*FC CS
Sbjct: 4091 KSWQVQAFNSWC*RTCLHRKCFLPCFIYVKSGN*FCDIRTACS 4219
Score = 31.3 bits (62), Expect(3) = 5e-033
Identities = 19/51 (37%), Positives = 26/51 (50%)
Frame = +2 / -2
Query: 269 VSKNTLPQSNKTSLLFRHK*RQRRDTPREYMLPHT*DCGLPYAMFSMSIMK 421
+ KNTL +KTS L HK* + +T E M +T + L AM +K
Sbjct: 4226 IRKNTLS*CHKTSFLI*HK*SKGGNTSGEDMFSNTKN*RLELAMILREFLK 4074
>gb|AV923029.1|AV923029 AV923029 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd3g22 5', mRNA sequence
Length = 611
Score = 189 bits (408), Expect(2) = 4e-067
Identities = 70/89 (78%), Positives = 85/89 (95%)
Frame = -1 / +2
Query: 268 LKVRMHYGHPDVFDRIFHITRGGISKASRVINISEDIYAGFNSTLRQGNITHHEYIQVGK 89
L+VR HYGHPD+FDR+FH+TRGG+SKAS++IN+SEDI+AGFNSTLR+GN+THHEY+QVGK
Sbjct: 140 LRVRFHYGHPDIFDRLFHLTRGGVSKASKIINLSEDIFAGFNSTLREGNVTHHEYMQVGK 319
Query: 88 GRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
GRDVGLNQI+LFE K+A GNGEQ LSRD+
Sbjct: 320 GRDVGLNQISLFEAKIAYGNGEQTLSRDI 406
Score = 118 bits (253), Expect(2) = 3e-043
Identities = 57/88 (64%), Positives = 61/88 (69%)
Frame = +3 / -3
Query: 6 SRLRTCSPLPPATFPSNSAIWFKPTSRPFPTWIYSWCVMFPWRKVELNPAYISSLILITR 185
SRLR CSPLP A F SNS I +PTS P PT +YS CV FP R VELNPA +SSL LI
Sbjct: 402 SRLRVCSPLPYAIFASNSEI*LRPTSLPLPTCMYS*CVTFPSRSVELNPANMSSLRLIIL 223
Query: 186 DALLMPPRVM*NIRSKTSGCP*CIRTLR 269
DALL PP V RSK SGCP* RTL+
Sbjct: 222 DALLTPPLVRWKSRSKISGCP**NRTLK 139
Score = 85.4 bits (180), Expect(2) = 4e-067
Identities = 36/42 (85%), Positives = 39/42 (92%)
Frame = -1 / +2
Query: 397 GIRKPTILGVREHVFTGSVSSLASFMSKQETSFVTLGQRVLA 272
G+R PTILGVREH+FTGSVSSLA FMS QETSFVT+GQRVLA
Sbjct: 8 GVRYPTILGVREHIFTGSVSSLAWFMSNQETSFVTIGQRVLA 133
Score = 76.2 bits (160), Expect(2) = 3e-043
Identities = 32/42 (76%), Positives = 34/42 (80%)
Frame = +3 / -3
Query: 273 ARTRCPKVTKLVSCFDINDASEETLPVNTCSLTPRIVGFRMP 398
A TRCP VTKLVS FD+N ASEETLPVN CSLTP IVG+ P
Sbjct: 132 ASTRCPIVTKLVSWFDMNHASEETLPVNICSLTPSIVGYLTP 7
>gb|BJ462458.1|BJ462458 BJ462458 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags28g06 5', mRNA sequence
Length = 666
Score = 185 bits (399), Expect(2) = 9e-050
Identities = 74/89 (83%), Positives = 82/89 (92%)
Frame = -1 / +3
Query: 268 LKVRMHYGHPDVFDRIFHITRGGISKASRVINISEDIYAGFNSTLRQGNITHHEYIQVGK 89
LKVRMHYGHPDVFDR++ + RGGISKASRVINISEDI+AGFN TLR GN+THHEYIQVGK
Sbjct: 69 LKVRMHYGHPDVFDRLWFLGRGGISKASRVINISEDIFAGFNCTLRGGNVTHHEYIQVGK 248
Query: 88 GRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
GRDVGLNQ+++FE KVA GNGEQ LSRDV
Sbjct: 249 GRDVGLNQVSMFEAKVASGNGEQTLSRDV 335
Score = 138 bits (296), Expect(2) = 8e-036
Identities = 59/88 (67%), Positives = 64/88 (72%)
Frame = +3 / -3
Query: 3 TSRLRTCSPLPPATFPSNSAIWFKPTSRPFPTWIYSWCVMFPWRKVELNPAYISSLILIT 182
TSRLR CSPLP AT SN W PTS P PTW+YSWCV P R+V+LNPA ISSL+LIT
Sbjct: 334 TSRLRVCSPLPLATLASNIETWLSPTSLPLPTWMYSWCVTLPPRRVQLNPAKISSLMLIT 155
Query: 183 RDALLMPPRVM*NIRSKTSGCP*CIRTL 266
DALL+PPR RS TSG P*CI TL
Sbjct: 154 LDALLIPPRPKNQRRSNTSGWP*CILTL 71
Score = 31.8 bits (63), Expect(2) = 8e-036
Identities = 12/13 (92%), Positives = 13/13 (100%)
Frame = +3 / -3
Query: 273 ARTRCPKVTKLVS 311
ARTRCP+VTKLVS
Sbjct: 61 ARTRCPRVTKLVS 23
Score = 31.3 bits (62), Expect(2) = 9e-050
Identities = 13/14 (92%), Positives = 14/14 (100%)
Frame = -1 / +3
Query: 313 QETSFVTLGQRVLA 272
+ETSFVTLGQRVLA
Sbjct: 21 RETSFVTLGQRVLA 62
>gb|BU969103.1|BU969103 HB10J09r BC Hordeum vulgare subsp. vulgare cDNA clone HB10J09
5-PRIME, mRNA sequence
Length = 426
Score = 137 bits (294), Expect = 6e-032
Identities = 56/58 (96%), Positives = 58/58 (100%)
Frame = -1 / +2
Query: 175 NISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
NISEDI+AGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRD+
Sbjct: 11 NISEDIFAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDI 184
Score = 108 bits (231), Expect = 3e-023
Identities = 45/58 (77%), Positives = 47/58 (81%)
Frame = +3 / -1
Query: 6 SRLRTCSPLPPATFPSNSAIWFKPTSRPFPTWIYSWCVMFPWRKVELNPAYISSLILI 179
S LRTCSP PPATFPSNSAI PTS P PTWIYSW VMFP R+VELNPA ISSLI +
Sbjct: 180 SLLRTCSPFPPATFPSNSAI*LSPTSLPLPTWIYSW*VMFP*RRVELNPANISSLIFL 7
>gb|AV836635.1|AV836635 AV836635 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd19k01, mRNA
sequence
Length = 644
Score = 121 bits (258), Expect = 5e-027
Identities = 49/50 (98%), Positives = 50/50 (100%)
Frame = -1 / +2
Query: 151 GFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
GFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRD+
Sbjct: 5 GFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDI 154
>gb|AV934137.1|AV934137 AV934137 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal11c09 5', mRNA sequence
Length = 589
Score = 120 bits (256), Expect = 1e-026
Identities = 46/57 (80%), Positives = 54/57 (94%)
Frame = -1 / +1
Query: 172 ISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
+SEDI+AGFNSTLR+GN+THHEY+QVGKGRDVGLNQI+LFE K+A GNGEQ LSRD+
Sbjct: 7 LSEDIFAGFNSTLREGNVTHHEYMQVGKGRDVGLNQISLFEAKIAYGNGEQTLSRDI 177
>gb|BJ470174.1|BJ470174 BJ470174 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal15l17 5', mRNA sequence
Length = 537
Score = 120 bits (256), Expect = 1e-026
Identities = 46/57 (80%), Positives = 54/57 (94%)
Frame = -1 / +3
Query: 172 ISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
+SEDI+AGFNSTLR+GN+THHEY+QVGKGRDVGLNQI+LFE K+A GNGEQ LSRD+
Sbjct: 18 LSEDIFAGFNSTLREGNVTHHEYMQVGKGRDVGLNQISLFEAKIAYGNGEQTLSRDI 188
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 290,219,648
Number of Sequences: 312970
Number of extensions: 4365664
Number of successful extensions: 35819
Number of sequences better than 1.0e-020: 10
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7550174202
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)