TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF36h01.yg.2.1
(689 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
dbj|AB040769.1| Hordeum vulgare mRNA for endo-1,4-beta-gluc... 480 e-135
gb|BE603205.3|BE603205 HVSMEh0102H23f Hordeum vulgare 5-45 ... 360 5e-099
gb|BJ448471.1|BJ448471 BJ448471 K. Sato unpublished cDNA li... 360 5e-099
gb|BI949643.1|BI949643 HVSMEl0015A22f Hordeum vulgare spike... 216 3e-089
gb|AW982666.3|AW982666 HVSMEg0003O04f Hordeum vulgare pre-a... 313 6e-085
gb|BG418753.1|BG418753 HVSMEk0024E15f Hordeum vulgare testa... 299 1e-080
gb|AV914917.1|AV914917 AV914917 K. Sato unpublished cDNA li... 275 2e-073
gb|BU985662.1|BU985662 HF08E02r HF Hordeum vulgare subsp. v... 237 4e-062
gb|BQ765008.1|BQ765008 EBca01_SQ005_D16_R carpel, pre-anthe... 168 1e-060
gb|BF260719.2|BF260719 HVSMEf0022M05f Hordeum vulgare seedl... 154 2e-056
gb|BU967340.1|BU967340 HB03P20r BC Hordeum vulgare subsp. v... 168 2e-056
gb|AV916435.1|AV916435 AV916435 K. Sato unpublished cDNA li... 214 4e-055
gb|BU979160.1|BU979160 HA15F07r HA Hordeum vulgare subsp. v... 201 3e-051
gb|BF255699.3|BF255699 HVSMEf0007J01f Hordeum vulgare seedl... 164 3e-046
gb|CK123474.1|CK123474 BES1824104c11 BES1824 Hordeum vulgar... 102 5e-046
gb|AV923747.1|AV923747 AV923747 K. Sato unpublished cDNA li... 183 7e-046
gb|AV909996.1|AV909996 AV909996 K. Sato unpublished cDNA li... 175 2e-043
gb|AL508501.1|AL508501 AL508501 Hordeum vulgare Barke devel... 167 2e-042
gb|BG365744.1|BG365744 HVSMEi0004A10f Hordeum vulgare 20 DA... 123 9e-040
gb|AW983004.3|AW983004 HVSMEg0004M14f Hordeum vulgare pre-a... 145 2e-034
gb|BU967676.1|BU967676 HB05B01r BC Hordeum vulgare subsp. v... 86 3e-030
gb|AV914894.1|AV914894 AV914894 K. Sato unpublished cDNA li... 122 1e-027
gb|BE601584.3|BE601584 HVSMEh0098M02f Hordeum vulgare 5-45 ... 106 1e-022
gb|BE455799.3|BE455799 HVSMEg0015D01f Hordeum vulgare pre-a... 105 3e-022
gb|BM097444.2|BM097444 EBro05_SQ003_F03_R root, 3 week, red... 101 3e-021
>dbj|AB040769.1| Hordeum vulgare mRNA for endo-1,4-beta-glucanase Cel1, complete cds
Length = 2442
Score = 480 bits (1042), Expect = e-135
Identities = 189/217 (87%), Positives = 196/217 (90%)
Frame = -2 / +1
Query: 652 EXIDYXXXXXECHSCSDLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYKFGRLQRGR 473
E IDY ECHSCSDLA A ASIVFKDSK YSDKLV GAKALYKFGRLQRGR
Sbjct: 937 EDIDYKRPVIECHSCSDLAAEMAAALAAASIVFKDSKAYSDKLVHGAKALYKFGRLQRGR 1116
Query: 472 YSPNGSDQAIFYNSTSYWDEFVWGGAWMYFATGNNSYLTVATAPGMAKHAGAYWFGSPNY 293
YSPNGSDQ++FYNSTSYWDEFVWGGAWMYFATGN SYLT+ATAPGMAKHAGA+W GSPNY
Sbjct: 1117 YSPNGSDQSLFYNSTSYWDEFVWGGAWMYFATGNTSYLTIATAPGMAKHAGAFWIGSPNY 1296
Query: 292 GVFTWDDKLPGAQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTKGG 113
GVFTWDDKLPG+QVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTKGG
Sbjct: 1297 GVFTWDDKLPGSQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTKGG 1476
Query: 112 LIQLNHGRPQPLQYAVNAAFLASLYSDYLEAADTPGW 2
LIQLNHG PQPLQY VNAAFLASLY+DYL+ ADTPGW
Sbjct: 1477 LIQLNHGGPQPLQYVVNAAFLASLYADYLDTADTPGW 1587
Score = 369 bits (800), Expect = e-102
Identities = 161/217 (74%), Positives = 167/217 (76%)
Frame = +3 / -2
Query: 3 HPGVSAASR*SLYKEARKAALTAY*SG*GLPWLSCINPPLVKLNELNTGKYEHMTLSVWL 182
HPGVSA SR*S Y+EAR AALT Y*SG*G PWLSCI+PPLVKLNELNTGKYEHMTLSVWL
Sbjct: 1586 HPGVSAVSR*SAYREARNAALTTY*SG*GPPWLSCISPPLVKLNELNTGKYEHMTLSVWL 1407
Query: 183 WNVLSISS*GYPGXXXXXXXXXXT*APGSLSSQVNTP*FGLPNQYAPACFAIPGAVATVR 362
WNVL+ISS*GYPG T* PGSLSSQVNTP*FGLP Q APAC AIPGAVA VR
Sbjct: 1406 WNVLNISS*GYPGLRKSRNLLRRT*DPGSLSSQVNTP*FGLPIQNAPACLAIPGAVAIVR 1227
Query: 363 YELFPVAKYIHAPPHTNSSQ*LVEL*KMAWSDPLGLYRPRCSLPNLYRALAPXXXXXXXX 542
Y++FPVAKYIHAPPHTNSSQ*LVEL* WS+PLGLYRPRCSLPNLY ALAP
Sbjct: 1226 YDVFPVAKYIHAPPHTNSSQ*LVEL*NSDWSEPLGLYRPRCSLPNLYSALAPWTSLSEYA 1047
Query: 543 XXXXNTMEAXXXXAXXXXARSEQEWHSXXXXX*SMXS 653
NTMEA A ARSEQEWHS *SM S
Sbjct: 1046 LLSLNTMEAAARAAAISAARSEQEWHSMTGLL*SMSS 936
Score = 310 bits (672), Expect = 4e-084
Identities = 138/217 (63%), Positives = 158/217 (72%)
Frame = +2 / -1
Query: 2 PPRRVSRFKIVAV*RSKESCVDSVLKRLRPSMVELY*SSFGEVE*IEYR*IRAHDVVGLV 181
PP + +I+++ R KE C+D VLK LRPSMVELY SSF EVE*IEY *I AHD+V LV
Sbjct: 1587 PPWCICSVQIISIQRGKECCIDDVLKWLRPSMVELYQSSFSEVE*IEYW*I*AHDIVSLV 1408
Query: 182 VECPQYFFVRVPWT*EESQPAEKNLSSWKFVIPGKHSVVWTSKPVCSSVLRHSRGCGDGE 361
VECP YFF+R+PW *EE QPAEKNL SWK VIPG+HS+VWT+ P VL H GD E
Sbjct: 1407 VECP*YFFIRIPWA*EEPQPAEKNLRSWKLVIPGEHSIVWTTNPERPRVLGHPWSGGDRE 1228
Query: 362 VRVVPRGEVHPRTAPHELVPVAGGVVEDGLVRSVGAVSPTLQPAELVQGLGALDELVGVG 541
VR VPRGEVHPR PH+L+PVAGG+VE LVR++GAVSP LQP ELVQ LG +D+LV V
Sbjct: 1227 VRCVPRGEVHPRATPHKLIPVAGGIVEQRLVRAIGAVSPPLQPTELVQRLGTVDKLVRVR 1048
Query: 542 LAVLEHYGSXXXXXXXXXR*I*AGVALXXXXXVVDVL 652
LAVLEHYGS I AGVAL VV+VL
Sbjct: 1047 LAVLEHYGSRGEGGGHLSGKIRAGVALDDRPLVVNVL 937
Score = 204 bits (440), Expect(2) = 8e-055
Identities = 94/171 (54%), Positives = 106/171 (61%)
Frame = +1 / -3
Query: 1 TTQACQPLQDSRCIKKQGKLR*QRTEAVEAFHG*AVLILLW*S*MN*IQVNTST*RCRFG 180
TT D++ ++QG L *+RTE VEA HG* V +LL *S*MN*I VN ST* C+FG
Sbjct: 1588 TTLVYLQCPDNQHTERQGMLH*RRTEVVEALHG*VVSVLL**S*MN*ILVNMST*HCQFG 1409
Query: 181 CGMSSVFLRKGTLDLGRVAAC*EEPELLEVCHPR*TLRSLDFQTSMLQRASPFPGLWRR* 360
CGMS +FL K TL LGR A C*EEPE+LE CHPR*TL SLD+Q+ RA P WR *
Sbjct: 1408 CGMSLIFLHKDTLGLGRAATC*EEPEILEACHPR*TLHSLDYQSRTPPRAWPSLERWRS* 1229
Query: 361 GTSCXXXXXXXXXXXXXXXXXXXWSCRRWPGQIRWGCIAHAAACRTCTGPW 513
GT C W+CR GQ WGCIA AAA RTCT PW
Sbjct: 1228 GTMCSPWRSTSTRHPTQTHPSSWWNCRTATGQSHWGCIAPAAAYRTCTAPW 1076
Score = 152 bits (327), Expect(3) = 1e-040
Identities = 66/87 (75%), Positives = 70/87 (80%)
Frame = -3 / +2
Query: 336 WRSTLEHTGLEVQTTECLPGMTNFQELRFFSAGCDSS*VQGTLTKKY*GHSTTKPTTSCA 157
W ST +GL VQT EC PGMT+FQ+LRFFSAGC SS* QG L KKY*GHSTTK T SCA
Sbjct: 1253 WPSTRGRSGLVVQTMECSPGMTSFQDLRFFSAGCGSS*AQGILMKKY*GHSTTKLTMSCA 1432
Query: 156 RIYLYSIHSTSPKED*YSSTMEGLNRF 76
IY YSIHSTS KED*Y+STMEGLN F
Sbjct: 1433 HIYQYSIHSTSLKED*YNSTMEGLNHF 1513
Score = 151 bits (324), Expect = 4e-036
Identities = 69/123 (56%), Positives = 79/123 (64%)
Frame = -1 / +3
Query: 395 VDVLRHGEQLVPHRRHSPGNGEARWSILVWKSKLRSVYLXXXXXXXXXXXXXXXTLPKSR 216
VDVLRHGE +VPH RH +G+AR +L W SKL SV+L LPK R
Sbjct: 1194 VDVLRHGEHIVPHDRHRSRDGQARGGVLDW*SKLWSVHLG*QASRISGSSQQVAALPKPR 1373
Query: 215 VPLRRNTEDIPQPNRQRHVLVFTCIQFIQLHQRRINTAQPWKASTASVRCQRSFPCFFIQ 36
V L RN +DIPQPN Q HVL+FT IQFIQLH RR +T QPW+AST SVR Q S PC +
Sbjct: 1374 VSL*RNIKDIPQPN*QCHVLIFTSIQFIQLH*RRTDTTQPWRASTTSVRRQCSIPCLSVC 1553
Query: 35 RLS 27
LS
Sbjct: 1554 *LS 1562
Score = 29.5 bits (58), Expect(2) = 8e-055
Identities = 15/43 (34%), Positives = 16/43 (37%)
Frame = +1 / -3
Query: 556 TLWKLXXXXXXXXTLDLSRSGTXXXXXCSRCXLXXXXXXXXXA 684
TLWK D SRSGT CS+C L A
Sbjct: 1033 TLWKPRRGRRPSQRQDPSRSGTR*QASCSQCPLVSSSSSGHSA 905
Score = 27.2 bits (53), Expect(3) = 1e-040
Identities = 12/29 (41%), Positives = 12/29 (41%)
Frame = -3 / +2
Query: 489 GCSVGDTAPTDLTRXXXXXXXXXXXXSCG 403
GCS GDTAP LT CG
Sbjct: 1100 GCSGGDTAPMALTSRCSTIPPATGMSLCG 1186
Score = 24.4 bits (47), Expect(3) = 1e-040
Identities = 9/11 (81%), Positives = 10/11 (90%)
Frame = -1 / +3
Query: 566 FHSVQGQQDLL 534
FHSVQGQQ +L
Sbjct: 1023 FHSVQGQQGVL 1055
>gb|BE603205.3|BE603205 HVSMEh0102H23f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0102H23f, mRNA sequence
Length = 726
Score = 360 bits (780), Expect = 5e-099
Identities = 137/149 (91%), Positives = 144/149 (96%)
Frame = -2 / +1
Query: 448 AIFYNSTSYWDEFVWGGAWMYFATGNNSYLTVATAPGMAKHAGAYWFGSPNYGVFTWDDK 269
++FYNSTSYWDEFVWGGAWMYFATGN SYLT+ATAPGMAKHAGA+W GSPNYGVFTWDDK
Sbjct: 16 SLFYNSTSYWDEFVWGGAWMYFATGNTSYLTIATAPGMAKHAGAFWIGSPNYGVFTWDDK 195
Query: 268 LPGAQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTKGGLIQLNHGR 89
LPG+QVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTKGGLIQLNHG
Sbjct: 196 LPGSQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTKGGLIQLNHGG 375
Query: 88 PQPLQYAVNAAFLASLYSDYLEAADTPGW 2
PQPLQY VNAAFLASLY+DYL+ ADTPGW
Sbjct: 376 PQPLQYVVNAAFLASLYADYLDTADTPGW 462
Score = 278 bits (602), Expect = 2e-074
Identities = 121/146 (82%), Positives = 126/146 (86%)
Frame = +3 / -2
Query: 3 HPGVSAASR*SLYKEARKAALTAY*SG*GLPWLSCINPPLVKLNELNTGKYEHMTLSVWL 182
HPGVSA SR*S Y+EAR AALT Y*SG*G PWLSCI+PPLVKLNELNTGKYEHMTLSVWL
Sbjct: 461 HPGVSAVSR*SAYREARNAALTTY*SG*GPPWLSCISPPLVKLNELNTGKYEHMTLSVWL 282
Query: 183 WNVLSISS*GYPGXXXXXXXXXXT*APGSLSSQVNTP*FGLPNQYAPACFAIPGAVATVR 362
WNVL+ISS*GYPG T* PGSLSSQVNTP*FGLP Q APAC AIPGAVA VR
Sbjct: 281 WNVLNISS*GYPGLRKSRNLLRRT*DPGSLSSQVNTP*FGLPIQNAPACLAIPGAVAIVR 102
Query: 363 YELFPVAKYIHAPPHTNSSQ*LVEL* 440
Y++FPVAKYIHAPPHTNSSQ*LVEL*
Sbjct: 101 YDVFPVAKYIHAPPHTNSSQ*LVEL* 24
Score = 241 bits (521), Expect = 3e-063
Identities = 100/150 (66%), Positives = 115/150 (76%)
Frame = +2 / -1
Query: 2 PPRRVSRFKIVAV*RSKESCVDSVLKRLRPSMVELY*SSFGEVE*IEYR*IRAHDVVGLV 181
PP + +I+++ R KE C+D VLK LRPSMVELY SSF EVE*IEY *IRAHD+V LV
Sbjct: 462 PPWCICSVQIISIQRGKECCIDDVLKWLRPSMVELYQSSFSEVE*IEYW*IRAHDIVSLV 283
Query: 182 VECPQYFFVRVPWT*EESQPAEKNLSSWKFVIPGKHSVVWTSKPVCSSVLRHSRGCGDGE 361
VECP YFF+R+PW *EE QPAEKNL SWK VIPG+HS+VWT+ P VL H GD E
Sbjct: 282 VECP*YFFIRIPWA*EEPQPAEKNLRSWKLVIPGEHSIVWTTNPERPRVLGHPWSGGDRE 103
Query: 362 VRVVPRGEVHPRTAPHELVPVAGGVVEDGL 451
VR VPRGEVHPR PH+L+PVAGG+VE L
Sbjct: 102 VRCVPRGEVHPRATPHKLIPVAGGIVEQRL 13
Score = 166 bits (358), Expect = 8e-041
Identities = 79/147 (53%), Positives = 91/147 (61%)
Frame = +1 / -3
Query: 1 TTQACQPLQDSRCIKKQGKLR*QRTEAVEAFHG*AVLILLW*S*MN*IQVNTST*RCRFG 180
TT D++ ++QG L *+RTE VEA HG* V +LL *S*MN*I VNTST* C+FG
Sbjct: 463 TTLVYLQCPDNQHTERQGMLH*RRTEVVEALHG*VVSVLL**S*MN*ILVNTST*HCQFG 284
Query: 181 CGMSSVFLRKGTLDLGRVAAC*EEPELLEVCHPR*TLRSLDFQTSMLQRASPFPGLWRR* 360
CGMS +FL K TL LGR A C*EEPE+LE CHPR*TL SLD+Q+ RA P WR *
Sbjct: 283 CGMSLIFLHKDTLGLGRAATC*EEPEILEACHPR*TLHSLDYQSRTPPRAWPSLERWRS* 104
Query: 361 GTSCXXXXXXXXXXXXXXXXXXXWSCR 441
GT C W+CR
Sbjct: 103 GTMCSPWRSTSTRHPTQTHPSSWWNCR 23
Score = 155 bits (332), Expect = 3e-037
Identities = 67/87 (77%), Positives = 71/87 (81%)
Frame = -3 / +2
Query: 336 WRSTLEHTGLEVQTTECLPGMTNFQELRFFSAGCDSS*VQGTLTKKY*GHSTTKPTTSCA 157
W ST +GL VQT EC PGMT+FQ+LRFFSAGC SS* QG L KKY*GHSTTK T SCA
Sbjct: 128 WPSTRGRSGLVVQTMECSPGMTSFQDLRFFSAGCGSS*AQGILMKKY*GHSTTKLTMSCA 307
Query: 156 RIYLYSIHSTSPKED*YSSTMEGLNRF 76
RIY YSIHSTS KED*Y+STMEGLN F
Sbjct: 308 RIYQYSIHSTSLKED*YNSTMEGLNHF 388
Score = 151 bits (325), Expect = 3e-036
Identities = 70/123 (56%), Positives = 79/123 (64%)
Frame = -1 / +3
Query: 395 VDVLRHGEQLVPHRRHSPGNGEARWSILVWKSKLRSVYLXXXXXXXXXXXXXXXTLPKSR 216
VDVLRHGE +VPH RH +G+AR +L W SKL SV+L LPK R
Sbjct: 69 VDVLRHGEHIVPHDRHRSRDGQARGGVLDW*SKLWSVHLG*QASRISGSSQQVAALPKPR 248
Query: 215 VPLRRNTEDIPQPNRQRHVLVFTCIQFIQLHQRRINTAQPWKASTASVRCQRSFPCFFIQ 36
V L RN +DIPQPN Q HVLVFT IQFIQLH RR +T QPW+AST SVR Q S PC +
Sbjct: 249 VSL*RNIKDIPQPN*QCHVLVFTSIQFIQLH*RRTDTTQPWRASTTSVRRQCSIPCLSVC 428
Query: 35 RLS 27
LS
Sbjct: 429 *LS 437
>gb|BJ448471.1|BJ448471 BJ448471 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak20b09 5', mRNA sequence
Length = 617
Score = 360 bits (780), Expect = 5e-099
Identities = 137/149 (91%), Positives = 144/149 (96%)
Frame = -2 / +2
Query: 448 AIFYNSTSYWDEFVWGGAWMYFATGNNSYLTVATAPGMAKHAGAYWFGSPNYGVFTWDDK 269
++FYNSTSYWDEFVWGGAWMYFATGN SYLT+ATAPGMAKHAGA+W GSPNYGVFTWDDK
Sbjct: 11 SLFYNSTSYWDEFVWGGAWMYFATGNTSYLTIATAPGMAKHAGAFWIGSPNYGVFTWDDK 190
Query: 268 LPGAQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTKGGLIQLNHGR 89
LPG+QVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTKGGLIQLNHG
Sbjct: 191 LPGSQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTKGGLIQLNHGG 370
Query: 88 PQPLQYAVNAAFLASLYSDYLEAADTPGW 2
PQPLQY VNAAFLASLY+DYL+ ADTPGW
Sbjct: 371 PQPLQYVVNAAFLASLYADYLDTADTPGW 457
Score = 278 bits (602), Expect = 2e-074
Identities = 121/146 (82%), Positives = 126/146 (86%)
Frame = +3 / -3
Query: 3 HPGVSAASR*SLYKEARKAALTAY*SG*GLPWLSCINPPLVKLNELNTGKYEHMTLSVWL 182
HPGVSA SR*S Y+EAR AALT Y*SG*G PWLSCI+PPLVKLNELNTGKYEHMTLSVWL
Sbjct: 456 HPGVSAVSR*SAYREARNAALTTY*SG*GPPWLSCISPPLVKLNELNTGKYEHMTLSVWL 277
Query: 183 WNVLSISS*GYPGXXXXXXXXXXT*APGSLSSQVNTP*FGLPNQYAPACFAIPGAVATVR 362
WNVL+ISS*GYPG T* PGSLSSQVNTP*FGLP Q APAC AIPGAVA VR
Sbjct: 276 WNVLNISS*GYPGLRKSRNLLRRT*DPGSLSSQVNTP*FGLPIQNAPACLAIPGAVAIVR 97
Query: 363 YELFPVAKYIHAPPHTNSSQ*LVEL* 440
Y++FPVAKYIHAPPHTNSSQ*LVEL*
Sbjct: 96 YDVFPVAKYIHAPPHTNSSQ*LVEL* 19
Score = 233 bits (503), Expect = 8e-061
Identities = 98/150 (65%), Positives = 113/150 (75%)
Frame = +2 / -2
Query: 2 PPRRVSRFKIVAV*RSKESCVDSVLKRLRPSMVELY*SSFGEVE*IEYR*IRAHDVVGLV 181
PP + +I+++ R KE C+D VLK LRPSMVELY SSF EVE*IEY *I AHD+V LV
Sbjct: 457 PPWCICSVQIISIQRGKECCIDDVLKWLRPSMVELYQSSFSEVE*IEYW*I*AHDIVSLV 278
Query: 182 VECPQYFFVRVPWT*EESQPAEKNLSSWKFVIPGKHSVVWTSKPVCSSVLRHSRGCGDGE 361
VECP YFF+R+PW *EE QPAEKNL SWK VIPG+HS+VWT+ P VL H GD E
Sbjct: 277 VECP*YFFIRIPWA*EEPQPAEKNLRSWKLVIPGEHSIVWTTNPERPRVLGHPWSGGDRE 98
Query: 362 VRVVPRGEVHPRTAPHELVPVAGGVVEDGL 451
V VPRGEVHPR PH+L+PVAGG+VE L
Sbjct: 97 V*CVPRGEVHPRATPHKLIPVAGGIVEQRL 8
Score = 161 bits (346), Expect = 4e-039
Identities = 77/147 (52%), Positives = 89/147 (60%)
Frame = +1 / -1
Query: 1 TTQACQPLQDSRCIKKQGKLR*QRTEAVEAFHG*AVLILLW*S*MN*IQVNTST*RCRFG 180
TT D++ ++QG L *+RTE VEA HG* V +LL *S*MN*I VN ST* C+FG
Sbjct: 458 TTLVYLQCPDNQHTERQGMLH*RRTEVVEALHG*VVSVLL**S*MN*ILVNMST*HCQFG 279
Query: 181 CGMSSVFLRKGTLDLGRVAAC*EEPELLEVCHPR*TLRSLDFQTSMLQRASPFPGLWRR* 360
CGMS +FL K TL LGR A C*EEPE+LE CHPR*TL SLD+Q+ RA P WR *
Sbjct: 278 CGMSLIFLHKDTLGLGRAATC*EEPEILEACHPR*TLHSLDYQSRTPPRAWPSLERWRS* 99
Query: 361 GTSCXXXXXXXXXXXXXXXXXXXWSCR 441
G C W+CR
Sbjct: 98 GMMCSPWRSTSTRHPTQTHPSSWWNCR 18
Score = 152 bits (327), Expect = 2e-036
Identities = 66/87 (75%), Positives = 70/87 (80%)
Frame = -3 / +3
Query: 336 WRSTLEHTGLEVQTTECLPGMTNFQELRFFSAGCDSS*VQGTLTKKY*GHSTTKPTTSCA 157
W ST +GL VQT EC PGMT+FQ+LRFFSAGC SS* QG L KKY*GHSTTK T SCA
Sbjct: 123 WPSTRGRSGLVVQTMECSPGMTSFQDLRFFSAGCGSS*AQGILMKKY*GHSTTKLTMSCA 302
Query: 156 RIYLYSIHSTSPKED*YSSTMEGLNRF 76
IY YSIHSTS KED*Y+STMEGLN F
Sbjct: 303 HIYQYSIHSTSLKED*YNSTMEGLNHF 383
Score = 150 bits (323), Expect = 6e-036
Identities = 68/123 (55%), Positives = 79/123 (64%)
Frame = -1 / +1
Query: 395 VDVLRHGEQLVPHRRHSPGNGEARWSILVWKSKLRSVYLXXXXXXXXXXXXXXXTLPKSR 216
VDVLRHGE ++PH RH +G+AR +L W SKL SV+L LPK R
Sbjct: 64 VDVLRHGEHIIPHDRHRSRDGQARGGVLDW*SKLWSVHLG*QASRISGSSQQVAALPKPR 243
Query: 215 VPLRRNTEDIPQPNRQRHVLVFTCIQFIQLHQRRINTAQPWKASTASVRCQRSFPCFFIQ 36
V L RN +DIPQPN Q HVL+FT IQFIQLH RR +T QPW+AST SVR Q S PC +
Sbjct: 244 VSL*RNIKDIPQPN*QCHVLIFTSIQFIQLH*RRTDTTQPWRASTTSVRRQCSIPCLSVC 423
Query: 35 RLS 27
LS
Sbjct: 424 *LS 432
>gb|BI949643.1|BI949643 HVSMEl0015A22f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0015A22f, mRNA sequence
Length = 902
Score = 216 bits (467), Expect(3) = 3e-089
Identities = 85/100 (85%), Positives = 92/100 (92%)
Frame = -2 / +3
Query: 301 PNYGVFTWDDKLPGAQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFT 122
PNYGVF+WD+KLPGAQVLLSRLRLFLSP YPYEE+LRT+HNQT +MCSYLP F SFN T
Sbjct: 369 PNYGVFSWDNKLPGAQVLLSRLRLFLSPXYPYEEMLRTYHNQTSTIMCSYLPDFRSFNRT 548
Query: 121 KGGLIQLNHGRPQPLQYAVNAAFLASLYSDYLEAADTPGW 2
KGGLIQLNHG+PQPLQY VNAAFLASL+SDYLEAADTP W
Sbjct: 549 KGGLIQLNHGQPQPLQYVVNAAFLASLFSDYLEAADTPWW 668
Score = 121 bits (259), Expect(3) = 2e-043
Identities = 59/100 (59%), Positives = 67/100 (67%)
Frame = +3 / -2
Query: 3 HPGVSAASR*SLYKEARKAALTAY*SG*GLPWLSCINPPLVKLNELNTGKYEHMTLSVWL 182
H GVSAASR*SL +EAR AALT Y SG G P +CI PP V L +L +G+ EHM + V L
Sbjct: 667 HQGVSAASR*SLNREARNAALTTYWSGCGCP*FNCIKPPFVLLKDLKSGR*EHMMVLV*L 488
Query: 183 WNVLSISS*GYPGXXXXXXXXXXT*APGSLSSQVNTP*FG 302
W VL+ISS GY G T APGSL SQ+NTP*FG
Sbjct: 487 WYVLNISSYGYXGLKKSLNRLRRTCAPGSLLSQLNTP*FG 368
Score = 85.8 bits (181), Expect(3) = 3e-089
Identities = 32/56 (57%), Positives = 40/56 (71%)
Frame = -2 / +3
Query: 481 RGRYSPNGSDQAIFYNSTSYWDEFVWGGAWMYFATGNNSYLTVATAPGMAKHAGAY 314
R YS SD A FYNST YWDE++WGG+WMY ATGN+SYL AT+ +AK+ +
Sbjct: 192 RSAYSALRSDAAKFYNSTGYWDEYMWGGSWMYLATGNSSYLQFATSVELAKNVHVF 359
Score = 84.9 bits (179), Expect(3) = 1e-024
Identities = 39/99 (39%), Positives = 61/99 (61%)
Frame = +2 / -1
Query: 2 PPRRVSRFKIVAV*RSKESCVDSVLKRLRPSMVELY*SSFGEVE*IEYR*IRAHDVVGLV 181
PP + KIV RSKE +D +L+RL +++LY ++F V+ + +RAHD GL+
Sbjct: 668 PPGGICSLKIVTEQRSKECSIDYILERLWLPVIQLYQTTFCSVKRSKIWKVRAHDGAGLI 489
Query: 182 VECPQYFFVRVPWT*EESQPAEKNLSSWKFVIPGKHSVV 298
V CP + F+ + T EE PA+KNL + + +IP +H +V
Sbjct: 488 VVCP*HLFIWIXRTQEEP*PAQKNLCTRELIIPAEHPIV 372
Score = 65.2 bits (136), Expect(3) = 3e-089
Identities = 26/55 (47%), Positives = 33/55 (60%)
Frame = -2 / +3
Query: 652 EXIDYXXXXXECHSCSDLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYKFGR 488
E IDY ECH+C DL A ASIVFKD++ YS KL+ GA +++F R
Sbjct: 15 EDIDYPRPVTECHTCPDLGAEMAAALAAASIVFKDNRAYSHKLLHGATTVWEFAR 179
Score = 58.3 bits (121), Expect(3) = 2e-043
Identities = 29/51 (56%), Positives = 31/51 (60%)
Frame = +3 / -2
Query: 330 FAIPGAVATVRYELFPVAKYIHAPPHTNSSQ*LVEL*KMAWSDPLGLYRPR 482
FA VA RY+ PVA+YIH PPH SSQ* VEL* A SD LY R
Sbjct: 343 FASSTLVANCRYDELPVARYIHEPPHIYSSQ*PVEL*NFAASDLSALYAER 191
Score = 39.1 bits (79), Expect(3) = 1e-024
Identities = 15/28 (53%), Positives = 19/28 (67%)
Frame = +2 / -1
Query: 359 EVRVVPRGEVHPRTAPHELVPVAGGVVE 442
+VR + EVHPRT PH +PV G +VE
Sbjct: 314 QVR*IASCEVHPRTTPHIFIPVTG*IVE 231
Score = 34.5 bits (69), Expect(3) = 2e-043
Identities = 19/55 (34%), Positives = 21/55 (38%)
Frame = +3 / -2
Query: 489 LPNLYRALAPXXXXXXXXXXXXNTMEAXXXXAXXXXARSEQEWHSXXXXX*SMXS 653
L N +AP NTM+A A RS Q WHS *SM S
Sbjct: 178 LANSQTVVAPCNSLWE*ALLSLNTMDAAANAAAISAPRSGQVWHSVTGRG*SMSS 14
Score = 26.3 bits (51), Expect(3) = 1e-024
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = +2 / -1
Query: 479 TLQPAELVQGLGALDELVGVGLAVLEHYG 565
T P +L G + +LVGV +LEH G
Sbjct: 188 TSLPCKLPDGGSTM*QLVGVSPIILEHNG 102
>gb|AW982666.3|AW982666 HVSMEg0003O04f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0003O04f, mRNA sequence
Length = 898
Score = 313 bits (678), Expect = 6e-085
Identities = 123/147 (83%), Positives = 129/147 (87%)
Frame = -2 / +1
Query: 652 EXIDYXXXXXECHSCSDLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYKFGRLQRGR 473
E IDY ECHSCSDLA A ASIVFKDSK YSDKLV GAKALYKFGRLQRGR
Sbjct: 49 EDIDYKRPVIECHSCSDLAAEMAAALAAASIVFKDSKAYSDKLVHGAKALYKFGRLQRGR 228
Query: 472 YSPNGSDQAIFYNSTSYWDEFVWGGAWMYFATGNNSYLTVATAPGMAKHAGAYWFGSPNY 293
YSPNGSDQ++FYNSTSYWDEFVWGGAWMYFATGN SYLT+ATAPGMAKHAGA+W GSPNY
Sbjct: 229 YSPNGSDQSLFYNSTSYWDEFVWGGAWMYFATGNTSYLTIATAPGMAKHAGAFWIGSPNY 408
Query: 292 GVFTWDDKLPGAQVLLSRLRLFLSPGY 212
GVFTWDDKLPG+QVLLSRLRLFLSPG+
Sbjct: 409 GVFTWDDKLPGSQVLLSRLRLFLSPGF 489
Score = 213 bits (460), Expect = 7e-055
Identities = 97/146 (66%), Positives = 100/146 (68%)
Frame = +3 / -3
Query: 216 PGXXXXXXXXXXT*APGSLSSQVNTP*FGLPNQYAPACFAIPGAVATVRYELFPVAKYIH 395
PG T* PGSLSSQVNTP*FGLP Q APAC AIPGAVA VRY++FPVAKYIH
Sbjct: 485 PGLRKSRNLLRRT*DPGSLSSQVNTP*FGLPIQNAPACLAIPGAVAIVRYDVFPVAKYIH 306
Query: 396 APPHTNSSQ*LVEL*KMAWSDPLGLYRPRCSLPNLYRALAPXXXXXXXXXXXXNTMEAXX 575
APPHTNSSQ*LVEL* S+PLGLYRPRCSLPNLY ALAP NTMEA
Sbjct: 305 APPHTNSSQ*LVEL*NSD*SEPLGLYRPRCSLPNLYSALAPWTSLSEYALLSLNTMEAAA 126
Query: 576 XXAXXXXARSEQEWHSXXXXX*SMXS 653
A ARSEQEWHS *SM S
Sbjct: 125 RAAAISAARSEQEWHSMTGLL*SMSS 48
Score = 207 bits (446), Expect = 6e-053
Identities = 92/151 (60%), Positives = 105/151 (69%)
Frame = +2 / -2
Query: 200 FFVRVPWT*EESQPAEKNLSSWKFVIPGKHSVVWTSKPVCSSVLRHSRGCGDGEVRVVPR 379
F + PW *EE QPAEKNL SWK VIPG+HS+VWT+ P VL H GD EVR VPR
Sbjct: 501 FSHKEPWA*EEPQPAEKNLRSWKLVIPGEHSIVWTTNPERPRVLGHPWSGGDREVRCVPR 322
Query: 380 GEVHPRTAPHELVPVAGGVVEDGLVRSVGAVSPTLQPAELVQGLGALDELVGVGLAVLEH 559
GEVHPR PH+L+PVAGG+VE L+R++GAVSP LQP ELVQ LG +D+LV V LAVLEH
Sbjct: 321 GEVHPRATPHKLIPVAGGIVEQRLIRAIGAVSPPLQPTELVQRLGTVDKLVRVRLAVLEH 142
Query: 560 YGSXXXXXXXXXR*I*AGVALXXXXXVVDVL 652
YGS I AGVAL VV+VL
Sbjct: 141 YGSRGEGGGHLSGKIRAGVALDDRPLVVNVL 49
Score = 123 bits (263), Expect(2) = 1e-030
Identities = 53/101 (52%), Positives = 57/101 (56%)
Frame = +1 / -1
Query: 211 GTLDLGRVAAC*EEPELLEVCHPR*TLRSLDFQTSMLQRASPFPGLWRR*GTSCXXXXXX 390
GTL LGR A C*EEPE+LE CHPR*TL SLD+Q+ RA P WR *GT C
Sbjct: 490 GTLGLGRAATC*EEPEILEACHPR*TLHSLDYQSRTPPRAWPSLERWRS*GTMCSPWRST 311
Query: 391 XXXXXXXXXXXXXWSCRRWPGQIRWGCIAHAAACRTCTGPW 513
W+CR Q WGCIA AAA RTCT PW
Sbjct: 310 STRHPTQTHPSSWWNCRTATDQSHWGCIAPAAAYRTCTAPW 188
Score = 29.5 bits (58), Expect(2) = 1e-030
Identities = 15/43 (34%), Positives = 16/43 (37%)
Frame = +1 / -1
Query: 556 TLWKLXXXXXXXXTLDLSRSGTXXXXXCSRCXLXXXXXXXXXA 684
TLWK D SRSGT CS+C L A
Sbjct: 145 TLWKPRRGRRPSQRQDPSRSGTR*QASCSQCPLVSSSSSGHSA 17
>gb|BG418753.1|BG418753 HVSMEk0024E15f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0024E15f, mRNA sequence
Length = 879
Score = 299 bits (647), Expect = 1e-080
Identities = 122/159 (76%), Positives = 129/159 (81%)
Frame = -2 / +3
Query: 652 EXIDYXXXXXECHSCSDLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYKFGRLQRGR 473
E IDY ECHSCSDLA A ASIVFKDSK YSDKLV GAKALYKFGRLQRGR
Sbjct: 258 EDIDYKRPVIECHSCSDLAAEMAAALAAASIVFKDSKAYSDKLVHGAKALYKFGRLQRGR 437
Query: 472 YSPNGSDQAIFYNSTSYWDEFVWGGAWMYFATGNNSYLTVATAPGMAKHAGAYWFGSPNY 293
YSPNGSDQ++FYNSTSY DEFVWGGAWMYFATGN SYLT+ATA MAKHAGA+W GSPNY
Sbjct: 438 YSPNGSDQSLFYNSTSYXDEFVWGGAWMYFATGNTSYLTIATASRMAKHAGAFWIGSPNY 617
Query: 292 GVFTWDDKLPGAQVLLSRLRLFLSPGYPYEEILRTFHNQ 176
GVFTWD +L +Q LLSRLRLFLSP Y Y ILRTFH +
Sbjct: 618 GVFTWDXQLSXSQGLLSRLRLFLSPRYSYGGILRTFHTK 734
Score = 206 bits (444), Expect(3) = 7e-056
Identities = 93/157 (59%), Positives = 107/157 (68%)
Frame = +2 / -2
Query: 182 VECPQYFFVRVPWT*EESQPAEKNLSSWKFVIPGKHSVVWTSKPVCSSVLRHSRGCGDGE 361
VECP Y +R+PW *EE QPAEK L S K IPG+HS+VWT+ P VL H GD E
Sbjct: 728 VECP*YSSIRIPWA*EEPQPAEKTLRS*KLXIPGEHSIVWTTNPERPRVLGHP*SGGDRE 549
Query: 362 VRVVPRGEVHPRTAPHELVPVAGGVVEDGLVRSVGAVSPTLQPAELVQGLGALDELVGVG 541
VR VPRGEVHPR PH+L+ VAGG+VE L+R++GAVSP LQP ELVQ LG +D+LV V
Sbjct: 548 VRCVPRGEVHPRATPHKLIXVAGGIVEQRLIRAIGAVSPPLQPTELVQRLGTVDKLVRVR 369
Query: 542 LAVLEHYGSXXXXXXXXXR*I*AGVALXXXXXVVDVL 652
LAVLEHYGS I AGVAL VV+VL
Sbjct: 368 LAVLEHYGSRGEGGGHLSGKIRAGVALDDRPLVVNVL 258
Score = 204 bits (439), Expect = 6e-052
Identities = 97/158 (61%), Positives = 102/158 (64%)
Frame = +3 / -3
Query: 180 LWNVLSISS*GYPGXXXXXXXXXXT*APGSLSSQVNTP*FGLPNQYAPACFAIPGAVATV 359
+WNVL+I * Y G * S SSQVNTP*FGLP Q APAC AI AVA V
Sbjct: 730 VWNVLNIPP*EYLGLRKSRNLLRRP*DXESXSSQVNTP*FGLPIQNAPACLAILEAVAIV 551
Query: 360 RYELFPVAKYIHAPPHTNSSQ*LVEL*KMAWSDPLGLYRPRCSLPNLYRALAPXXXXXXX 539
RY++FPVAKYIHAPPHTNSSQ*LVEL* S+PLGLYRPRCSLPNLY ALAP
Sbjct: 550 RYDVFPVAKYIHAPPHTNSSQ*LVEL*NSD*SEPLGLYRPRCSLPNLYSALAPWTSLSEY 371
Query: 540 XXXXXNTMEAXXXXAXXXXARSEQEWHSXXXXX*SMXS 653
NTMEA A ARSEQEWHS *SM S
Sbjct: 370 ALLSLNTMEAAARAAAISAARSEQEWHSMTGLL*SMSS 257
Score = 124 bits (265), Expect(2) = 7e-031
Identities = 55/111 (49%), Positives = 62/111 (55%)
Frame = +1 / -1
Query: 181 CGMSSVFLRKGTLDLGRVAAC*EEPELLEVCHPR*TLRSLDFQTSMLQRASPFPGLWRR* 360
CGMS +FL K TL LGR A C*E+ E+ + HPR*TL SLD+Q+ RA P WR *
Sbjct: 729 CGMSLIFLHKNTLGLGRAATC*EDLEIXKAXHPR*TLHSLDYQSRTPPRAWPSLKRWRS* 550
Query: 361 GTSCXXXXXXXXXXXXXXXXXXXWSCRRWPGQIRWGCIAHAAACRTCTGPW 513
GT C W+CR Q WGCIA AAA RTCT PW
Sbjct: 549 GTMCSPWRSTSTRHPTQTHXSSWWNCRTATDQSHWGCIAPAAAYRTCTAPW 397
Score = 29.5 bits (58), Expect(2) = 7e-031
Identities = 15/43 (34%), Positives = 16/43 (37%)
Frame = +1 / -1
Query: 556 TLWKLXXXXXXXXTLDLSRSGTXXXXXCSRCXLXXXXXXXXXA 684
TLWK D SRSGT CS+C L A
Sbjct: 354 TLWKPRRGRRPSQRQDPSRSGTR*QASCSQCPLVSSSSSGHSA 226
Score = 27.6 bits (54), Expect(3) = 7e-056
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = +3 / -1
Query: 138 LNTGKYEHMTLSVW 179
LN GK HMT SVW
Sbjct: 771 LNXGKSSHMTWSVW 730
Score = 22.1 bits (42), Expect(3) = 7e-056
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = +3 / -1
Query: 42 KEARKAALTAY*SG* 86
++AR AALT +*SG*
Sbjct: 864 QKARDAALTTF*SG* 820
>gb|AV914917.1|AV914917 AV914917 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags9a10 5', mRNA sequence
Length = 618
Score = 275 bits (595), Expect = 2e-073
Identities = 107/129 (82%), Positives = 111/129 (86%)
Frame = -2 / +3
Query: 652 EXIDYXXXXXECHSCSDLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYKFGRLQRGR 473
E IDY ECHSCSDLA A ASIVFKDSK YSDKLV GAKALYKFGRLQRGR
Sbjct: 231 EDIDYKRPVIECHSCSDLAAEMAAALAAASIVFKDSKAYSDKLVHGAKALYKFGRLQRGR 410
Query: 472 YSPNGSDQAIFYNSTSYWDEFVWGGAWMYFATGNNSYLTVATAPGMAKHAGAYWFGSPNY 293
YSPNGSDQ++FYNSTSYWDEFVWGGAWMYFATGN SYLT+ATAPGMAKHAGA+W GSPNY
Sbjct: 411 YSPNGSDQSLFYNSTSYWDEFVWGGAWMYFATGNTSYLTIATAPGMAKHAGAFWIGSPNY 590
Query: 292 GVFTWDDKL 266
GVFTWDDKL
Sbjct: 591 GVFTWDDKL 617
Score = 204 bits (439), Expect = 6e-052
Identities = 91/129 (70%), Positives = 94/129 (72%)
Frame = +3 / -3
Query: 267 SLSSQVNTP*FGLPNQYAPACFAIPGAVATVRYELFPVAKYIHAPPHTNSSQ*LVEL*KM 446
SLSSQVNTP*FGLP Q APAC AIPGAVA VRY++FPVAKYIHAPPHTNSSQ*LVEL*
Sbjct: 616 SLSSQVNTP*FGLPIQNAPACLAIPGAVAIVRYDVFPVAKYIHAPPHTNSSQ*LVEL*NS 437
Query: 447 AWSDPLGLYRPRCSLPNLYRALAPXXXXXXXXXXXXNTMEAXXXXAXXXXARSEQEWHSX 626
S+PLGLYRPRCSLPNLY ALAP NTMEA A ARSEQEWHS
Sbjct: 436 D*SEPLGLYRPRCSLPNLYSALAPWTSLSEYALLSLNTMEAAARAAAISAARSEQEWHSM 257
Query: 627 XXXX*SMXS 653
*SM S
Sbjct: 256 TGLL*SMSS 230
Score = 171 bits (367), Expect = 5e-042
Identities = 77/129 (59%), Positives = 89/129 (68%)
Frame = +2 / -2
Query: 266 KFVIPGKHSVVWTSKPVCSSVLRHSRGCGDGEVRVVPRGEVHPRTAPHELVPVAGGVVED 445
K VIPG+HS+VWT+ P VL H GD EVR VPRGEVHPR PH+L+PVAGG+VE
Sbjct: 617 KLVIPGEHSIVWTTNPERPRVLGHPWSGGDREVRCVPRGEVHPRATPHKLIPVAGGIVEQ 438
Query: 446 GLVRSVGAVSPTLQPAELVQGLGALDELVGVGLAVLEHYGSXXXXXXXXXR*I*AGVALX 625
L+R++GAVSP LQP ELVQ LG +D+LV V LAVLEHYGS I AGVAL
Sbjct: 437 RLIRAIGAVSPPLQPTELVQRLGTVDKLVRVRLAVLEHYGSRGEGGGHLSGKIRAGVALD 258
Query: 626 XXXXVVDVL 652
VV+VL
Sbjct: 257 DRPLVVNVL 231
Score = 90.9 bits (192), Expect(2) = 6e-021
Identities = 39/83 (46%), Positives = 42/83 (50%)
Frame = +1 / -1
Query: 265 EVCHPR*TLRSLDFQTSMLQRASPFPGLWRR*GTSCXXXXXXXXXXXXXXXXXXXWSCRR 444
E CHPR*TL SLD+Q+ RA P WR *GT C W+CR
Sbjct: 618 EACHPR*TLHSLDYQSRTPPRAWPSLERWRS*GTMCSPWRSTSTRHPTQTHPSSWWNCRT 439
Query: 445 WPGQIRWGCIAHAAACRTCTGPW 513
Q WGCIA AAA RTCT PW
Sbjct: 438 ATDQSHWGCIAPAAAYRTCTAPW 370
Score = 29.5 bits (58), Expect(2) = 6e-021
Identities = 15/43 (34%), Positives = 16/43 (37%)
Frame = +1 / -1
Query: 556 TLWKLXXXXXXXXTLDLSRSGTXXXXXCSRCXLXXXXXXXXXA 684
TLWK D SRSGT CS+C L A
Sbjct: 327 TLWKPRRGRRPSQRQDPSRSGTR*QASCSQCPLVSSSSSGHSA 199
>gb|BU985662.1|BU985662 HF08E02r HF Hordeum vulgare subsp. vulgare cDNA clone HF08E02
5-PRIME, mRNA sequence
Length = 583
Score = 237 bits (513), Expect = 4e-062
Identities = 93/99 (93%), Positives = 96/99 (96%)
Frame = -2 / +3
Query: 298 NYGVFTWDDKLPGAQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTK 119
NYGVFTWDDKLPG+QVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTK
Sbjct: 12 NYGVFTWDDKLPGSQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTK 191
Query: 118 GGLIQLNHGRPQPLQYAVNAAFLASLYSDYLEAADTPGW 2
GGLIQLNHG PQPLQY VNAAFLASLY+DYL+ ADTPGW
Sbjct: 192 GGLIQLNHGGPQPLQYVVNAAFLASLYADYLDTADTPGW 308
Score = 184 bits (396), Expect = 5e-046
Identities = 80/99 (80%), Positives = 83/99 (83%)
Frame = +3 / -1
Query: 3 HPGVSAASR*SLYKEARKAALTAY*SG*GLPWLSCINPPLVKLNELNTGKYEHMTLSVWL 182
HPGVSA SR*S Y+EAR AALT Y*SG*G PWLSCI+PPLVKLNELNTGKYEHMTLSVWL
Sbjct: 307 HPGVSAVSR*SAYREARNAALTTY*SG*GPPWLSCISPPLVKLNELNTGKYEHMTLSVWL 128
Query: 183 WNVLSISS*GYPGXXXXXXXXXXT*APGSLSSQVNTP*F 299
WNVL+ISS*GYPG T* PGSLSSQVNTP*F
Sbjct: 127 WNVLNISS*GYPGLRKSRNLLRRT*DPGSLSSQVNTP*F 11
Score = 163 bits (350), Expect = 1e-039
Identities = 68/99 (68%), Positives = 79/99 (79%)
Frame = +2 / -3
Query: 2 PPRRVSRFKIVAV*RSKESCVDSVLKRLRPSMVELY*SSFGEVE*IEYR*IRAHDVVGLV 181
PP + +I+++ R KE C+D VLK LRPSMVELY SSF EVE*IEY *I AHD+V LV
Sbjct: 308 PPWCICSVQIISIQRGKECCIDDVLKWLRPSMVELYQSSFSEVE*IEYW*I*AHDIVSLV 129
Query: 182 VECPQYFFVRVPWT*EESQPAEKNLSSWKFVIPGKHSVV 298
VECP YFF+R+PW *EE QPAEKNL SWK VIPG+HS+V
Sbjct: 128 VECP*YFFIRIPWA*EEPQPAEKNLRSWKLVIPGEHSIV 12
Score = 138 bits (297), Expect = 2e-032
Identities = 59/75 (78%), Positives = 63/75 (84%)
Frame = -3 / +1
Query: 300 QTTECLPGMTNFQELRFFSAGCDSS*VQGTLTKKY*GHSTTKPTTSCARIYLYSIHSTSP 121
+T EC PGMT+FQ+LRFFSAGC SS* QG L KKY*GHSTTK T SCA IY YSIHSTS
Sbjct: 10 ETMECSPGMTSFQDLRFFSAGCGSS*AQGILMKKY*GHSTTKLTMSCAHIYQYSIHSTSL 189
Query: 120 KED*YSSTMEGLNRF 76
KED*Y+STMEGLN F
Sbjct: 190 KED*YNSTMEGLNHF 234
Score = 135 bits (289), Expect = 3e-031
Identities = 63/99 (63%), Positives = 72/99 (72%)
Frame = +1 / -2
Query: 1 TTQACQPLQDSRCIKKQGKLR*QRTEAVEAFHG*AVLILLW*S*MN*IQVNTST*RCRFG 180
TT D++ ++QG L *+RTE VEA HG* V +LL *S*MN*I VN ST* C+FG
Sbjct: 309 TTLVYLQCPDNQHTERQGMLH*RRTEVVEALHG*VVSVLL**S*MN*ILVNMST*HCQFG 130
Query: 181 CGMSSVFLRKGTLDLGRVAAC*EEPELLEVCHPR*TLRS 297
CGMS +FL K TL LGR A C*EEPE+LE CHPR*TL S
Sbjct: 129 CGMSLIFLHKDTLGLGRAATC*EEPEILEACHPR*TLHS 13
Score = 105 bits (223), Expect = 4e-022
Identities = 50/91 (54%), Positives = 56/91 (61%)
Frame = -1 / +2
Query: 299 KLRSVYLXXXXXXXXXXXXXXXTLPKSRVPLRRNTEDIPQPNRQRHVLVFTCIQFIQLHQ 120
KL SV+L LPK RV L RN +DIPQPN Q HVL+FT IQFIQLH
Sbjct: 11 KLWSVHLG*QASRISGSSQQVAALPKPRVSL*RNIKDIPQPN*QCHVLIFTSIQFIQLH* 190
Query: 119 RRINTAQPWKASTASVRCQRSFPCFFIQRLS 27
RR +T QPW+AST SVR Q S PC + LS
Sbjct: 191 RRTDTTQPWRASTTSVRRQCSIPCLSVC*LS 283
>gb|BQ765008.1|BQ765008 EBca01_SQ005_D16_R carpel, pre-anthesis, no treatment, cv Optic,
EBca01 Hordeum vulgare subsp. vulgare cDNA clone
EBca01_SQ005_D16 5', mRNA sequence
Length = 685
Score = 168 bits (362), Expect(2) = 1e-060
Identities = 64/104 (61%), Positives = 81/104 (77%)
Frame = -2 / +2
Query: 313 WFGSPNYGVFTWDDKLPGAQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNS 134
+F ++ VF+WD+KLPGA +LLSRLR+FL+PGYPYEE L +HN T MC Y P FN+
Sbjct: 242 YFNILDFSVFSWDNKLPGATLLLSRLRMFLNPGYPYEESLMGYHNVTSMNMCMYFPKFNA 421
Query: 133 FNFTKGGLIQLNHGRPQPLQYAVNAAFLASLYSDYLEAADTPGW 2
FNFTKGGL Q NHG+ Q LQYAV +FLA+LY+DY+E+ + PGW
Sbjct: 422 FNFTKGGLAQFNHGQGQALQYAVANSFLAALYADYMESVNVPGW 553
Score = 89.5 bits (189), Expect(2) = 8e-027
Identities = 47/95 (49%), Positives = 52/95 (54%)
Frame = +3 / -2
Query: 6 PGVSAASR*SLYKEARKAALTAY*SG*GLPWLSCINPPLVKLNELNTGKYEHMTLSVWLW 185
PG S *S ARK TAY S PWL+ +PPLVKLN LN GKY HM + V LW
Sbjct: 549 PGTLTDSM*SA*SAARKELATAYCSAWPWPWLNWASPPLVKLNALNLGKYMHMFMLVTLW 370
Query: 186 NVLSISS*GYPGXXXXXXXXXXT*APGSLSSQVNT 290
+S SS*G PG APGSL SQ+ T
Sbjct: 369 YPMSDSS*G*PGLRNMRSRDSSRVAPGSLLSQLKT 265
Score = 84.9 bits (179), Expect(2) = 1e-060
Identities = 31/58 (53%), Positives = 41/58 (70%)
Frame = -2 / +2
Query: 484 QRGRYSPNGSDQAIFYNSTSYWDEFVWGGAWMYFATGNNSYLTVATAPGMAKHAGAYW 311
+R YS +YNSTSYWDEF+W +WMY+ATGNNSY++ AT P + K+A AY+
Sbjct: 74 KRTPYSRGNPAIEYYYNSTSYWDEFMWSASWMYYATGNNSYISFATDPRLPKNAKAYF 247
Score = 50.6 bits (104), Expect(2) = 8e-027
Identities = 21/30 (70%), Positives = 23/30 (76%)
Frame = +2 / -1
Query: 350 GDGEVRVVPRGEVHPRTAPHELVPVAGGVV 439
G+ +V VV G VHP APHELVPVAGGVV
Sbjct: 208 GEADVAVVAGGVVHPGGAPHELVPVAGGVV 119
>gb|BF260719.2|BF260719 HVSMEf0022M05f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0022M05f, mRNA sequence
Length = 541
Score = 154 bits (331), Expect(2) = 2e-056
Identities = 60/98 (61%), Positives = 76/98 (77%)
Frame = -2 / +2
Query: 313 WFGSPNYGVFTWDDKLPGAQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNS 134
+F ++ VF+WD+KLPGA +LLSRLR+FL+PGYPYEE L +HN T MC Y P FN+
Sbjct: 242 YFNILDFSVFSWDNKLPGATLLLSRLRMFLNPGYPYEESLMGYHNVTSMNMCMYFPKFNA 421
Query: 133 FNFTKGGLIQLNHGRPQPLQYAVNAAFLASLYSDYLEA 20
FNFTKGGL Q NHG+ LQYAV +FLA+LY+DY+E+
Sbjct: 422 FNFTKGGLAQFNHGQCHALQYAVANSFLAALYADYMES 535
Score = 85.8 bits (181), Expect(2) = 1e-025
Identities = 45/89 (50%), Positives = 50/89 (56%)
Frame = +3 / -2
Query: 24 SR*SLYKEARKAALTAY*SG*GLPWLSCINPPLVKLNELNTGKYEHMTLSVWLWNVLSIS 203
S *S ARK TAY S PWL+ +PPLVKLN LN GKY HM + V LW +S S
Sbjct: 531 SM*SA*SAARKELATAYCSAWHXPWLNWASPPLVKLNALNLGKYMHMFMLVTLWYPMSDS 352
Query: 204 S*GYPGXXXXXXXXXXT*APGSLSSQVNT 290
S*G PG APGSL SQ+ T
Sbjct: 351 S*G*PGLRNMRSRDSSRVAPGSLLSQLKT 265
Score = 84.9 bits (179), Expect(2) = 2e-056
Identities = 31/58 (53%), Positives = 41/58 (70%)
Frame = -2 / +2
Query: 484 QRGRYSPNGSDQAIFYNSTSYWDEFVWGGAWMYFATGNNSYLTVATAPGMAKHAGAYW 311
+R YS +YNSTSYWDEF+W +WMY+ATGNNSY++ AT P + K+A AY+
Sbjct: 74 KRTPYSRGNPAIEYYYNSTSYWDEFMWSASWMYYATGNNSYISFATDPRLPKNAKAYF 247
Score = 50.6 bits (104), Expect(2) = 1e-025
Identities = 21/30 (70%), Positives = 23/30 (76%)
Frame = +2 / -1
Query: 350 GDGEVRVVPRGEVHPRTAPHELVPVAGGVV 439
G+ +V VV G VHP APHELVPVAGGVV
Sbjct: 208 GEADVAVVAGGVVHPGGAPHELVPVAGGVV 119
>gb|BU967340.1|BU967340 HB03P20r BC Hordeum vulgare subsp. vulgare cDNA clone HB03P20
5-PRIME, mRNA sequence
Length = 523
Score = 168 bits (362), Expect(2) = 2e-056
Identities = 64/104 (61%), Positives = 81/104 (77%)
Frame = -2 / +2
Query: 313 WFGSPNYGVFTWDDKLPGAQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNS 134
+F ++ VF+WD+KLPGA +LLSRLR+FL+PGYPYEE L +HN T MC Y P FN+
Sbjct: 137 YFNILDFSVFSWDNKLPGATLLLSRLRMFLNPGYPYEESLMGYHNVTSMNMCMYFPKFNA 316
Query: 133 FNFTKGGLIQLNHGRPQPLQYAVNAAFLASLYSDYLEAADTPGW 2
FNFTKGGL Q NHG+ Q LQYAV +FLA+LY+DY+E+ + PGW
Sbjct: 317 FNFTKGGLAQFNHGQGQALQYAVANSFLAALYADYMESVNVPGW 448
Score = 89.5 bits (189), Expect(2) = 4e-025
Identities = 47/95 (49%), Positives = 52/95 (54%)
Frame = +3 / -2
Query: 6 PGVSAASR*SLYKEARKAALTAY*SG*GLPWLSCINPPLVKLNELNTGKYEHMTLSVWLW 185
PG S *S ARK TAY S PWL+ +PPLVKLN LN GKY HM + V LW
Sbjct: 444 PGTLTDSM*SA*SAARKELATAYCSAWPWPWLNWASPPLVKLNALNLGKYMHMFMLVTLW 265
Query: 186 NVLSISS*GYPGXXXXXXXXXXT*APGSLSSQVNT 290
+S SS*G PG APGSL SQ+ T
Sbjct: 264 YPMSDSS*G*PGLRNMRSRDSSRVAPGSLLSQLKT 160
Score = 70.3 bits (147), Expect(2) = 2e-056
Identities = 25/41 (60%), Positives = 33/41 (80%)
Frame = -2 / +2
Query: 433 STSYWDEFVWGGAWMYFATGNNSYLTVATAPGMAKHAGAYW 311
STSYWDEF+ +WMY+ATGNNSY++ AT P + K+A AY+
Sbjct: 20 STSYWDEFMGSASWMYYATGNNSYISFATDPRLPKNAKAYF 142
Score = 45.1 bits (92), Expect(2) = 4e-025
Identities = 19/28 (67%), Positives = 20/28 (71%)
Frame = +2 / -1
Query: 350 GDGEVRVVPRGEVHPRTAPHELVPVAGG 433
G+ V VV G VHP APHELVPVAGG
Sbjct: 103 GEAYVAVVAGGVVHPGGAPHELVPVAGG 20
>gb|AV916435.1|AV916435 AV916435 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags17d08 5', mRNA sequence
Length = 607
Score = 214 bits (462), Expect = 4e-055
Identities = 85/91 (93%), Positives = 88/91 (96%)
Frame = -2 / +3
Query: 274 DKLPGAQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTKGGLIQLNH 95
DKLPG+QVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTKGGLIQLNH
Sbjct: 27 DKLPGSQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTKGGLIQLNH 206
Query: 94 GRPQPLQYAVNAAFLASLYSDYLEAADTPGW 2
G PQPLQY VNAAFLASLY+DYL+ ADTPGW
Sbjct: 207 GGPQPLQYVVNAAFLASLYADYLDTADTPGW 299
Score = 166 bits (358), Expect = 8e-041
Identities = 72/91 (79%), Positives = 75/91 (82%)
Frame = +3 / -1
Query: 3 HPGVSAASR*SLYKEARKAALTAY*SG*GLPWLSCINPPLVKLNELNTGKYEHMTLSVWL 182
HPGVSA SR*S Y+EAR AALT Y*SG*G PWLSCI+PPLVKLNELNTGKYEHMTLSVWL
Sbjct: 298 HPGVSAVSR*SAYREARNAALTTY*SG*GPPWLSCISPPLVKLNELNTGKYEHMTLSVWL 119
Query: 183 WNVLSISS*GYPGXXXXXXXXXXT*APGSLS 275
WNVL+ISS*GYPG T* PGSLS
Sbjct: 118 WNVLNISS*GYPGLRKSRNLLRRT*DPGSLS 26
Score = 149 bits (319), Expect = 2e-035
Identities = 63/93 (67%), Positives = 72/93 (77%)
Frame = +2 / -3
Query: 2 PPRRVSRFKIVAV*RSKESCVDSVLKRLRPSMVELY*SSFGEVE*IEYR*IRAHDVVGLV 181
PP + +I+++ R KE C+D VLK LRPSMVELY SSF EVE*IEY *I AHD+V LV
Sbjct: 299 PPWCICSVQIISIQRGKECCIDDVLKWLRPSMVELYQSSFSEVE*IEYW*I*AHDIVSLV 120
Query: 182 VECPQYFFVRVPWT*EESQPAEKNLSSWKFVIP 280
VECP YFF+R+PW *EE QPAEKNL SWK V P
Sbjct: 119 VECP*YFFIRIPWA*EEPQPAEKNLRSWKLVXP 21
Score = 127 bits (272), Expect = 6e-029
Identities = 55/68 (80%), Positives = 58/68 (85%)
Frame = -3 / +1
Query: 279 GMTNFQELRFFSAGCDSS*VQGTLTKKY*GHSTTKPTTSCARIYLYSIHSTSPKED*YSS 100
GMT+FQ+LRFFSAGC SS* QG L KKY*GHSTTK T SCA IY YSIHSTS KED*Y+S
Sbjct: 22 GMTSFQDLRFFSAGCGSS*AQGILMKKY*GHSTTKLTMSCAHIYQYSIHSTSLKED*YNS 201
Query: 99 TMEGLNRF 76
TMEGLN F
Sbjct: 202 TMEGLNHF 225
Score = 123 bits (263), Expect = 1e-027
Identities = 57/92 (61%), Positives = 66/92 (71%)
Frame = +1 / -2
Query: 1 TTQACQPLQDSRCIKKQGKLR*QRTEAVEAFHG*AVLILLW*S*MN*IQVNTST*RCRFG 180
TT D++ ++QG L *+RTE VEA HG* V +LL *S*MN*I VN ST* C+FG
Sbjct: 300 TTLVYLQCPDNQHTERQGMLH*RRTEVVEALHG*VVSVLL**S*MN*ILVNMST*HCQFG 121
Query: 181 CGMSSVFLRKGTLDLGRVAAC*EEPELLEVCH 276
CGMS +FL K TL LGR A C*EEPE+LE CH
Sbjct: 120 CGMSLIFLHKDTLGLGRAATC*EEPEILEACH 25
Score = 101 bits (215), Expect = 4e-021
Identities = 45/68 (66%), Positives = 50/68 (73%)
Frame = -1 / +2
Query: 230 LPKSRVPLRRNTEDIPQPNRQRHVLVFTCIQFIQLHQRRINTAQPWKASTASVRCQRSFP 51
LPK RV L RN +DIPQPN Q HVL+FT IQFIQLH RR +T QPW+AST SVR Q S P
Sbjct: 71 LPKPRVSL*RNIKDIPQPN*QCHVLIFTSIQFIQLH*RRTDTTQPWRASTTSVRRQCSIP 250
Query: 50 CFFIQRLS 27
C + LS
Sbjct: 251 CLSVC*LS 274
>gb|BU979160.1|BU979160 HA15F07r HA Hordeum vulgare subsp. vulgare cDNA clone HA15F07
5-PRIME, mRNA sequence
Length = 443
Score = 201 bits (434), Expect = 3e-051
Identities = 80/92 (86%), Positives = 86/92 (93%)
Frame = -2 / +1
Query: 277 DDKLPGAQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTKGGLIQLN 98
D+KLPGAQVLLSRLRLFLSPGYPYEE+LRT+HNQT +MCSYLP F SFN TKGGLIQLN
Sbjct: 10 DNKLPGAQVLLSRLRLFLSPGYPYEEMLRTYHNQTSTIMCSYLPDFRSFNRTKGGLIQLN 189
Query: 97 HGRPQPLQYAVNAAFLASLYSDYLEAADTPGW 2
HG+PQPLQY VNAAFLASL+SDYLEAADTPGW
Sbjct: 190 HGQPQPLQYVVNAAFLASLFSDYLEAADTPGW 285
Score = 112 bits (239), Expect = 2e-024
Identities = 54/92 (58%), Positives = 61/92 (66%)
Frame = +3 / -1
Query: 3 HPGVSAASR*SLYKEARKAALTAY*SG*GLPWLSCINPPLVKLNELNTGKYEHMTLSVWL 182
HPGVSAASR*SL +EAR AALT Y SG G P +CI PP V L +L +G+ EHM + V L
Sbjct: 284 HPGVSAASR*SLNREARNAALTTYWSGCGCP*FNCIKPPFVLLKDLKSGR*EHMMVLV*L 105
Query: 183 WNVLSISS*GYPGXXXXXXXXXXT*APGSLSS 278
W VL+ISS GYPG T APGSL S
Sbjct: 104 WYVLNISSYGYPGLKKSLNRLRRTCAPGSLLS 9
>gb|BF255699.3|BF255699 HVSMEf0007J01f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0007J01f, mRNA sequence
Length = 833
Score = 164 bits (352), Expect(2) = 3e-046
Identities = 63/104 (60%), Positives = 79/104 (75%)
Frame = -2 / +3
Query: 313 WFGSPNYGVFTWDDKLPGAQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNS 134
+F ++ VF+WD+KLPGA +LLSRLR+FL+PGYPYEE L +HN T MC Y P FN+
Sbjct: 84 YFNILDFSVFSWDNKLPGATLLLSRLRMFLNPGYPYEESLMGYHNVTSMNMCMYFPKFNA 263
Query: 133 FNFTKGGLIQLNHGRPQPLQYAVNAAFLASLYSDYLEAADTPGW 2
FNFTKGGL Q NHG+ Q LQYAV + LA+LY+DY+E+ PGW
Sbjct: 264 FNFTKGGLAQFNHGQGQALQYAVANSLLAALYADYMESVIVPGW 395
Score = 40.9 bits (83), Expect(2) = 3e-046
Identities = 15/26 (57%), Positives = 21/26 (80%)
Frame = -2 / +3
Query: 388 YFATGNNSYLTVATAPGMAKHAGAYW 311
Y+ATGNNSY++ AT P + K+A AY+
Sbjct: 12 YYATGNNSYISFATDPRLPKNAKAYF 89
>gb|CK123474.1|CK123474 BES1824104c11 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010C114 5-PRIME, mRNA sequence
Length = 780
Score = 102 bits (217), Expect(2) = 5e-046
Identities = 36/40 (90%), Positives = 38/40 (95%)
Frame = -2 / +3
Query: 478 GRYSPNGSDQAIFYNSTSYWDEFVWGGAWMYFATGNNSYL 359
GRYSPNGSDQ++FYNSTSYWDEFVWGG WMYFATGN SYL
Sbjct: 660 GRYSPNGSDQSLFYNSTSYWDEFVWGGXWMYFATGNXSYL 779
Score = 102 bits (217), Expect(2) = 5e-046
Identities = 44/63 (69%), Positives = 44/63 (69%)
Frame = -2 / +2
Query: 652 EXIDYXXXXXECHSCSDLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYKFGRLQRGR 473
E IDY ECHSCSDLA A ASIVFKDSK YSDKLV GAKALYKFGRLQRG
Sbjct: 485 EDIDYKRPVIECHSCSDLAAEMAAALAAASIVFKDSKAYSDKLVHGAKALYKFGRLQRGA 664
Query: 472 YSP 464
P
Sbjct: 665 IQP 673
Score = 78.5 bits (165), Expect(2) = 2e-030
Identities = 29/40 (72%), Positives = 35/40 (87%)
Frame = +2 / -2
Query: 359 EVRVVPRGEVHPRTAPHELVPVAGGVVEDGLVRSVGAVSP 478
EVR VPRGEVHPR PH+L+PVAGG+VE L+R++GAVSP
Sbjct: 779 EVRXVPRGEVHPRATPHKLIPVAGGIVEQRLIRAIGAVSP 660
Score = 77.1 bits (162), Expect(2) = 4e-027
Identities = 32/40 (80%), Positives = 34/40 (85%)
Frame = +3 / -3
Query: 360 RYELFPVAKYIHAPPHTNSSQ*LVEL*KMAWSDPLGLYRP 479
RY+ FPVAKYIH PPHTNSSQ*LVEL* S+PLGLYRP
Sbjct: 778 RYDXFPVAKYIHXPPHTNSSQ*LVEL*NSD*SEPLGLYRP 659
Score = 73.5 bits (154), Expect(2) = 2e-030
Identities = 33/63 (52%), Positives = 33/63 (52%)
Frame = +3 / -1
Query: 465 GLYRPRCSLPNLYRALAPXXXXXXXXXXXXNTMEAXXXXAXXXXARSEQEWHSXXXXX*S 644
G PRCSLPNLY ALAP NTMEA A ARSEQEWHS *S
Sbjct: 672 GCIAPRCSLPNLYSALAPWTSLSEYALLSLNTMEAAARAAAISAARSEQEWHSMTGLL*S 493
Query: 645 MXS 653
M S
Sbjct: 492 MSS 484
Score = 63.8 bits (133), Expect(2) = 4e-027
Identities = 34/64 (53%), Positives = 38/64 (59%)
Frame = +2 / -3
Query: 461 VGAVSPTLQPAELVQGLGALDELVGVGLAVLEHYGSXXXXXXXXXR*I*AGVALXXXXXV 640
+G P LQP ELVQ LG +D+LV V LAVLEHYGS I AGVAL V
Sbjct: 676 LGLYRPPLQPTELVQRLGTVDKLVRVRLAVLEHYGSRGEGGGHLSGKIRAGVALDDRPLV 497
Query: 641 VDVL 652
V+VL
Sbjct: 496 VNVL 485
>gb|AV923747.1|AV923747 AV923747 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd1e17 5', mRNA sequence
Length = 507
Score = 183 bits (395), Expect = 7e-046
Identities = 71/77 (92%), Positives = 74/77 (96%)
Frame = -2 / +1
Query: 232 LFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTKGGLIQLNHGRPQPLQYAVNAAF 53
+FLSPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTKGGLIQLNHG PQPLQY VNAAF
Sbjct: 1 VFLSPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTKGGLIQLNHGGPQPLQYVVNAAF 180
Query: 52 LASLYSDYLEAADTPGW 2
LASLY+DYL+ ADTPGW
Sbjct: 181 LASLYADYLDTADTPGW 231
Score = 157 bits (338), Expect = 5e-038
Identities = 65/73 (89%), Positives = 68/73 (93%)
Frame = +3 / -2
Query: 3 HPGVSAASR*SLYKEARKAALTAY*SG*GLPWLSCINPPLVKLNELNTGKYEHMTLSVWL 182
HPGVSA SR*S Y+EAR AALT Y*SG*G PWLSCI+PPLVKLNELNTGKYEHMTLSVWL
Sbjct: 230 HPGVSAVSR*SAYREARNAALTTY*SG*GPPWLSCISPPLVKLNELNTGKYEHMTLSVWL 51
Query: 183 WNVLSISS*GYPG 221
WNVL+ISS*GYPG
Sbjct: 50 WNVLNISS*GYPG 12
Score = 118 bits (252), Expect = 4e-026
Identities = 50/77 (64%), Positives = 60/77 (77%)
Frame = +2 / -1
Query: 2 PPRRVSRFKIVAV*RSKESCVDSVLKRLRPSMVELY*SSFGEVE*IEYR*IRAHDVVGLV 181
PP + +I+++ R KE C+D VLK LRPSMVELY SSF EVE*IEY *I AHD+V LV
Sbjct: 231 PPWCICSVQIISIQRGKECCIDDVLKWLRPSMVELYQSSFSEVE*IEYW*I*AHDIVSLV 52
Query: 182 VECPQYFFVRVPWT*EE 232
VECP YFF+R+PW *E+
Sbjct: 51 VECP*YFFIRIPWA*ED 1
Score = 101 bits (215), Expect = 4e-021
Identities = 45/68 (66%), Positives = 50/68 (73%)
Frame = -1 / +3
Query: 230 LPKSRVPLRRNTEDIPQPNRQRHVLVFTCIQFIQLHQRRINTAQPWKASTASVRCQRSFP 51
LPK RV L RN +DIPQPN Q HVL+FT IQFIQLH RR +T QPW+AST SVR Q S P
Sbjct: 3 LPKPRVSL*RNIKDIPQPN*QCHVLIFTSIQFIQLH*RRTDTTQPWRASTTSVRRQCSIP 182
Query: 50 CFFIQRLS 27
C + LS
Sbjct: 183 CLSVC*LS 206
>gb|AV909996.1|AV909996 AV909996 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak2l16 5', mRNA sequence
Length = 253
Score = 175 bits (377), Expect = 2e-043
Identities = 68/74 (91%), Positives = 70/74 (94%)
Frame = -2 / +1
Query: 223 SPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTKGGLIQLNHGRPQPLQYAVNAAFLAS 44
SPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTKGGLIQLNHG PQPLQY VNAAFLAS
Sbjct: 1 SPGYPYEEILRTFHNQTDNVMCSYLPVFNSFNFTKGGLIQLNHGGPQPLQYVVNAAFLAS 180
Query: 43 LYSDYLEAADTPGW 2
LY+DYL+ A TPGW
Sbjct: 181 LYADYLDTAXTPGW 222
Score = 155 bits (334), Expect = 2e-037
Identities = 64/73 (87%), Positives = 67/73 (91%)
Frame = +3 / -3
Query: 3 HPGVSAASR*SLYKEARKAALTAY*SG*GLPWLSCINPPLVKLNELNTGKYEHMTLSVWL 182
HPGV A SR*S Y+EAR AALT Y*SG*G PWLSCI+PPLVKLNELNTGKYEHMTLSVWL
Sbjct: 221 HPGVXAVSR*SAYREARNAALTTY*SG*GPPWLSCISPPLVKLNELNTGKYEHMTLSVWL 42
Query: 183 WNVLSISS*GYPG 221
WNVL+ISS*GYPG
Sbjct: 41 WNVLNISS*GYPG 3
Score = 112 bits (240), Expect = 2e-024
Identities = 48/73 (65%), Positives = 56/73 (76%)
Frame = +2 / -2
Query: 2 PPRRVSRFKIVAV*RSKESCVDSVLKRLRPSMVELY*SSFGEVE*IEYR*IRAHDVVGLV 181
PP +I+++ R KE C+D VLK LRPSMVELY SSF EVE*IEY *I AHD+V LV
Sbjct: 222 PPWCXCSVQIISIQRGKECCIDDVLKWLRPSMVELYQSSFSEVE*IEYW*I*AHDIVSLV 43
Query: 182 VECPQYFFVRVPW 220
VECP YFF+R+PW
Sbjct: 42 VECP*YFFIRIPW 4
>gb|AL508501.1|AL508501 AL508501 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY08P04V 5',
mRNA sequence
Length = 692
Score = 167 bits (359), Expect(2) = 2e-042
Identities = 67/85 (78%), Positives = 69/85 (81%)
Frame = -2 / +1
Query: 652 EXIDYXXXXXECHSCSDLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYKFGRLQRGR 473
E IDY ECHSCSDLA A ASIVFKDSK YSDKLV GAKALYKFGRLQRGR
Sbjct: 385 EDIDYKRPVIECHSCSDLAAEMAAALAAASIVFKDSKAYSDKLVHGAKALYKFGRLQRGR 564
Query: 472 YSPNGSDQAIFYNSTSYWDEFVWGG 398
YSPNGSDQ++FYNSTSYWDEFVWGG
Sbjct: 565 YSPNGSDQSLFYNSTSYWDEFVWGG 639
Score = 116 bits (248), Expect(2) = 3e-028
Identities = 53/85 (62%), Positives = 54/85 (63%)
Frame = +3 / -1
Query: 399 PPHTNSSQ*LVEL*KMAWSDPLGLYRPRCSLPNLYRALAPXXXXXXXXXXXXNTMEAXXX 578
PPHTNSSQ*LVEL* S+PLGLYRPRCSLPNLY ALAP NTMEA
Sbjct: 638 PPHTNSSQ*LVEL*NSD*SEPLGLYRPRCSLPNLYSALAPWTSLSEYALLSLNTMEAAAR 459
Query: 579 XAXXXXARSEQEWHSXXXXX*SMXS 653
A ARSEQEWHS *SM S
Sbjct: 458 AAAISAARSEQEWHSMTGLL*SMSS 384
Score = 105 bits (223), Expect(2) = 4e-023
Identities = 49/83 (59%), Positives = 58/83 (69%)
Frame = +2 / -3
Query: 404 PHELVPVAGGVVEDGLVRSVGAVSPTLQPAELVQGLGALDELVGVGLAVLEHYGSXXXXX 583
PH+L+PVAGG+VE L+R++GAVSP LQP ELVQ LG +D+LV V LAVLEHYGS
Sbjct: 633 PHKLIPVAGGIVEQRLIRAIGAVSPPLQPTELVQRLGTVDKLVRVRLAVLEHYGSRGEGG 454
Query: 584 XXXXR*I*AGVALXXXXXVVDVL 652
I AGVAL VV+VL
Sbjct: 453 GHLSGKIRAGVALDDRPLVVNVL 385
Score = 28.6 bits (56), Expect(2) = 3e-028
Identities = 11/18 (61%), Positives = 12/18 (66%)
Frame = +2 / -2
Query: 368 VVPRGEVHPRTAPHELVP 421
V PRGEVHPR P + P
Sbjct: 670 VFPRGEVHPRGHPTQTHP 617
Score = 25.4 bits (49), Expect(2) = 2e-042
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = -2 / +2
Query: 397 AWMYFATGNN 368
AWMYFATG +
Sbjct: 641 AWMYFATGEH 670
Score = 22.6 bits (43), Expect(2) = 4e-023
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +3 / -3
Query: 375 PVAKYIHA 398
PVAKYIHA
Sbjct: 663 PVAKYIHA 640
>gb|BG365744.1|BG365744 HVSMEi0004A10f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0004A10f, mRNA sequence
Length = 484
Score = 123 bits (263), Expect(2) = 1e-038
Identities = 52/69 (75%), Positives = 53/69 (76%)
Frame = -2 / +3
Query: 652 EXIDYXXXXXECHSCSDLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYKFGRLQRGR 473
E IDY ECHSCSDLA A ASIVFKDSK YSDKLV GAKALYKFGRLQRGR
Sbjct: 75 EDIDYKRPVIECHSCSDLAAEMAAALAAASIVFKDSKAYSDKLVHGAKALYKFGRLQRGR 254
Query: 472 YSPNGSDQA 446
YSPNGSDQ+
Sbjct: 255 YSPNGSDQS 281
Score = 118 bits (252), Expect(2) = 1e-027
Identities = 49/78 (62%), Positives = 54/78 (69%)
Frame = -2 / +1
Query: 478 GRYSPNGSDQAIFYNSTSYWDEFVWGGAWMYFATGNNSYLTVATAPGMAKHAGAYWFGSP 299
G +P +FYNSTSYWDEFVWGG WMYF T N YLT+AT P MAKHA A GS
Sbjct: 250 GDTAPMALISQLFYNSTSYWDEFVWGGTWMYFDTENT*YLTIATGPRMAKHAEACSHGST 429
Query: 298 NYGVFTWDDKLPGAQVLL 245
NYGV+T DDKL +Q LL
Sbjct: 430 NYGVYTXDDKLTRSQDLL 483
Score = 93.6 bits (198), Expect(2) = 9e-040
Identities = 43/58 (74%), Positives = 47/58 (81%)
Frame = +3 / -3
Query: 267 SLSSQVNTP*FGLPNQYAPACFAIPGAVATVRYELFPVAKYIHAPPHTNSSQ*LVEL* 440
SLSSQV TP*F LP ++A AC AI G VA VRY +F V+KYIH PPHTNSSQ*LVEL*
Sbjct: 461 SLSSQVYTP*FVLPCEHASACLAILGPVAIVRYYVFSVSKYIHVPPHTNSSQ*LVEL* 288
Score = 90.0 bits (190), Expect(2) = 9e-040
Identities = 39/67 (58%), Positives = 40/67 (59%)
Frame = +3 / -1
Query: 453 SDPLGLYRPRCSLPNLYRALAPXXXXXXXXXXXXNTMEAXXXXAXXXXARSEQEWHSXXX 632
S+PLGLYRPRCSLPNLY ALAP NTMEA A ARSEQEWHS
Sbjct: 274 SEPLGLYRPRCSLPNLYSALAPWTSLSEYALLSLNTMEAAARAAAISAARSEQEWHSMTG 95
Query: 633 XX*SMXS 653
*SM S
Sbjct: 94 LL*SMSS 74
Score = 77.6 bits (163), Expect(2) = 3e-028
Identities = 39/68 (57%), Positives = 45/68 (66%)
Frame = +2 / -3
Query: 449 LVRSVGAVSPTLQPAELVQGLGALDELVGVGLAVLEHYGSXXXXXXXXXR*I*AGVALXX 628
L+R++GAVSP LQP ELVQ LG +D+LV V LAVLEHYGS I AGVAL
Sbjct: 278 LIRAIGAVSPPLQPTELVQRLGTVDKLVRVRLAVLEHYGSRGEGGGHLSGKIRAGVALDD 99
Query: 629 XXXVVDVL 652
VV+VL
Sbjct: 98 RPLVVNVL 75
Score = 67.5 bits (141), Expect(2) = 3e-028
Identities = 33/63 (52%), Positives = 39/63 (61%)
Frame = +2 / -2
Query: 254 LSSWKFVIPGKHSVVWTSKPVCSSVLRHSRGCGDGEVRVVPRGEVHPRTAPHELVPVAGG 433
L S + VI G HS+V T+ VL H GD EV V EVHPR APH+L+PVAGG
Sbjct: 474 LRSCQLVIXGVHSIVCTTMRARLCVLGHPWTGGDREVLCVLCVEVHPRAAPHKLIPVAGG 295
Query: 434 VVE 442
+VE
Sbjct: 294 IVE 286
Score = 56.5 bits (117), Expect(2) = 1e-038
Identities = 24/42 (57%), Positives = 29/42 (69%)
Frame = -1 / +3
Query: 404 GRCVDVLRHGEQLVPHRRHSPGNGEARWSILVWKSKLRSVYL 279
GR VDVLRH E +VPH RH +G+AR +L W KL SV+L
Sbjct: 324 GRHVDVLRHREHIVPHDRHRSKDGQARRGVLAW*YKLWSVHL 449
Score = 24.4 bits (47), Expect(2) = 1e-027
Identities = 9/11 (81%), Positives = 10/11 (90%)
Frame = -1 / +2
Query: 566 FHSVQGQQDLL 534
FHSVQGQQ +L
Sbjct: 161 FHSVQGQQGVL 193
>gb|AW983004.3|AW983004 HVSMEg0004M14f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0004M14f, mRNA sequence
Length = 562
Score = 145 bits (312), Expect = 2e-034
Identities = 56/61 (91%), Positives = 58/61 (95%)
Frame = -2 / +1
Query: 184 HNQTDNVMCSYLPVFNSFNFTKGGLIQLNHGRPQPLQYAVNAAFLASLYSDYLEAADTPG 5
HNQTDNVMCSYLPVFNSFNFTKGGLIQLNHG PQPLQY VNAAFLASLY+DYL+ ADTPG
Sbjct: 1 HNQTDNVMCSYLPVFNSFNFTKGGLIQLNHGGPQPLQYVVNAAFLASLYADYLDTADTPG 180
Query: 4 W 2
W
Sbjct: 181 W 183
Score = 127 bits (273), Expect = 4e-029
Identities = 53/60 (88%), Positives = 55/60 (91%)
Frame = +3 / -3
Query: 3 HPGVSAASR*SLYKEARKAALTAY*SG*GLPWLSCINPPLVKLNELNTGKYEHMTLSVWL 182
HPGVSA SR*S Y+EAR AALT Y*SG*G PWLSCI+PPLVKLNELNTGKYEHMTLSVWL
Sbjct: 182 HPGVSAVSR*SAYREARNAALTTY*SG*GPPWLSCISPPLVKLNELNTGKYEHMTLSVWL 3
>gb|BU967676.1|BU967676 HB05B01r BC Hordeum vulgare subsp. vulgare cDNA clone HB05B01
5-PRIME, mRNA sequence
Length = 363
Score = 85.8 bits (181), Expect(3) = 3e-030
Identities = 32/56 (57%), Positives = 40/56 (71%)
Frame = -2 / +3
Query: 481 RGRYSPNGSDQAIFYNSTSYWDEFVWGGAWMYFATGNNSYLTVATAPGMAKHAGAY 314
R YS SD A FYNST YWDE++WGG+WMY ATGN+SYL AT+ +AK+ +
Sbjct: 138 RSAYSALRSDAAKFYNSTGYWDEYMWGGSWMYLATGNSSYLQFATSVELAKNVHVF 305
Score = 42.3 bits (86), Expect(3) = 3e-030
Identities = 14/16 (87%), Positives = 16/16 (100%)
Frame = -2 / +3
Query: 301 PNYGVFTWDDKLPGAQ 254
PNYGVF+WD+KLPGAQ
Sbjct: 315 PNYGVFSWDNKLPGAQ 362
Score = 41.4 bits (84), Expect(3) = 3e-030
Identities = 17/38 (44%), Positives = 23/38 (60%)
Frame = -2 / +3
Query: 601 LAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYKFGR 488
L A ASIVFKD++ YS KL+ GA +++F R
Sbjct: 12 LGAEMAAALAAASIVFKDNRAYSHKLLHGATTVWEFAR 125
>gb|AV914894.1|AV914894 AV914894 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags8o19 5', mRNA sequence
Length = 562
Score = 122 bits (262), Expect = 1e-027
Identities = 52/68 (76%), Positives = 52/68 (76%)
Frame = -2 / +3
Query: 652 EXIDYXXXXXECHSCSDLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYKFGRLQRGR 473
E IDY ECHSCSDLA A ASIVFKDSK YSDKLV GAKALYKFGRLQRGR
Sbjct: 357 EDIDYKRPVIECHSCSDLAAEMAAALAAASIVFKDSKAYSDKLVHGAKALYKFGRLQRGR 536
Query: 472 YSPNGSDQ 449
YSPNGSDQ
Sbjct: 537 YSPNGSDQ 560
>gb|BE601584.3|BE601584 HVSMEh0098M02f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0098M02f, mRNA sequence
Length = 641
Score = 106 bits (226), Expect = 1e-022
Identities = 41/46 (89%), Positives = 43/46 (93%)
Frame = -2 / +1
Query: 139 NSFNFTKGGLIQLNHGRPQPLQYAVNAAFLASLYSDYLEAADTPGW 2
NSFNFTKGGLIQLNHG PQPLQY VNAAFLASLY+DYL+ ADTPGW
Sbjct: 1 NSFNFTKGGLIQLNHGGPQPLQYVVNAAFLASLYADYLDTADTPGW 138
>gb|BE455799.3|BE455799 HVSMEg0015D01f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0015D01f, mRNA sequence
Length = 578
Score = 105 bits (224), Expect = 3e-022
Identities = 45/63 (71%), Positives = 45/63 (71%)
Frame = -2 / +3
Query: 652 EXIDYXXXXXECHSCSDLAXXXXAXXXXASIVFKDSKTYSDKLVKGAKALYKFGRLQRGR 473
E IDY ECHSCSDLA A ASIVFKDSK YSDKLV GAKALYKFGRLQ GR
Sbjct: 366 EDIDYKRPVIECHSCSDLAAEMAAALAAASIVFKDSKAYSDKLVHGAKALYKFGRLQPGR 545
Query: 472 YSP 464
Y P
Sbjct: 546 YXP 554
>gb|BM097444.2|BM097444 EBro05_SQ003_F03_R root, 3 week, reduced light, cv Optic, EBro05
Hordeum vulgare subsp. vulgare cDNA clone
EBro05_SQ003_F03 5', mRNA sequence
Length = 499
Score = 101 bits (216), Expect = 3e-021
Identities = 39/44 (88%), Positives = 41/44 (93%)
Frame = -2 / +1
Query: 133 FNFTKGGLIQLNHGRPQPLQYAVNAAFLASLYSDYLEAADTPGW 2
FNFTKGGLIQLNHG PQPLQY VNAAFLASLY+DYL+ ADTPGW
Sbjct: 1 FNFTKGGLIQLNHGGPQPLQYVVNAAFLASLYADYLDTADTPGW 132
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 285,300,943
Number of Sequences: 312970
Number of extensions: 4292069
Number of successful extensions: 41955
Number of sequences better than 1.0e-020: 38
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7550174202
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)