TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF25f04.yg.3.3
(1599 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ469318.1|BJ469318 BJ469318 K. Sato unpublished cDNA li... 276 e-114
gb|BJ470140.1|BJ470140 BJ470140 K. Sato unpublished cDNA li... 276 e-111
gb|AV934080.1|AV934080 AV934080 K. Sato unpublished cDNA li... 261 e-101
gb|AV915093.1|AV915093 AV915093 K. Sato unpublished cDNA li... 332 e-100
gb|BJ473588.1|BJ473588 BJ473588 K. Sato unpublished cDNA li... 178 8e-045
gb|AV833703.1|AV833703 AV833703 K. Sato unpublished cDNA li... 157 1e-037
gb|BI779104.2|BI779104 EBro01_SQ003_A04_R root, 3 week, hyd... 152 5e-037
gb|AV920227.1|AV920227 AV920227 K. Sato unpublished cDNA li... 138 8e-033
gb|BJ474628.1|BJ474628 BJ474628 K. Sato unpublished cDNA li... 111 7e-025
gb|AV936330.1|AV936330 AV936330 K. Sato unpublished cDNA li... 111 7e-025
>gb|BJ469318.1|BJ469318 BJ469318 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal20e17 5', mRNA sequence
Length = 663
Score = 276 bits (597), Expect(2) = e-114
Identities = 115/126 (91%), Positives = 118/126 (93%)
Frame = +1 / +2
Query: 508 PEHRLKFFSLLRAIGTHCFQALIQLSSQQLKLVIDSINWAFRHTERNIAETGLSLLLEIL 687
PEHRLKFFSLLRAIGTHCF+ALIQLSSQQLKLVIDSINWAFRHTERNIAETGLSLLLEIL
Sbjct: 20 PEHRLKFFSLLRAIGTHCFKALIQLSSQQLKLVIDSINWAFRHTERNIAETGLSLLLEIL 199
Query: 688 KKFQASGFQNQFYKTYFLTIEQEIFAVLTDTFHKPGFKLHVSVLQHLFCAVDGLTEPLWD 867
K FQAS F NQFYKTYFL IEQEIFAVLTD+FHKPGFKLHV VLQHLFC VD LTEPLWD
Sbjct: 200 KNFQASEFTNQFYKTYFLNIEQEIFAVLTDSFHKPGFKLHVLVLQHLFCVVDSLTEPLWD 379
Query: 868 ASSVPY 885
AS+V Y
Sbjct: 380 ASTVSY 397
Score = 217 bits (469), Expect(2) = 6e-087
Identities = 104/130 (80%), Positives = 108/130 (83%)
Frame = -2 / -1
Query: 884 YGTEDASHRGSVRPSTAQNKCCSTET*SLKPGLWNVSVRTAKISCSIVKK*VL*NWFWNP 705
Y T DASHRGSVR ST QNKC ST+T*SL PGL N SVRTAKISCSI KK VL*N F N
Sbjct: 396 YDTVDASHRGSVRLSTTQNKC*STKT*SLNPGL*NESVRTAKISCSIFKKYVL*N*FVNS 217
Query: 704 EAWNFFKISNNRLRPVSAIFLSVCLNAQLIESITSFNC*LESWINAWKQWVPIARSKEKN 525
EA FF+ISNNRLRPVSA+FLSVCLNAQLIESITSFNC LES I A KQ VPIARSKEKN
Sbjct: 216 EA*KFFRISNNRLRPVSAMFLSVCLNAQLIESITSFNCWLES*IKALKQ*VPIARSKEKN 37
Query: 524 LRRCSG*SSK 495
LRRCSG S +
Sbjct: 36 LRRCSGSSCR 7
Score = 172 bits (371), Expect(2) = 1e-069
Identities = 78/130 (60%), Positives = 93/130 (71%)
Frame = +3 / +1
Query: 501 RLS*APPQIFFFASCNWHPLLPSINPAFKSAIEACDRFN*LGVQTYREKYCRDWP*PVVG 680
R S*APPQ+F F SC H LL S N AF+ AIEACD F+*LG+ TYREK+CRD P* +VG
Sbjct: 13 RGS*APPQVFLFTSCYRHSLLQSFNSAFQPAIEACD*FD*LGI*TYREKHCRDRP*SIVG 192
Query: 681 DLEKIPGFRIPKPILQNLLFDN*ARDFCSPHRHIP*TWFQASCFGATTLVLCG*WSNGAS 860
+ E+ GFRI + +LQ++ F+ A DFCS HR I *TW Q SCFGA+TLVL G* N S
Sbjct: 193 NSEEFSGFRIHESVLQDIFFEYRAGDFCSSHRFIS*TWIQTSCFGASTLVLRG*QPN*TS 372
Query: 861 MGRVFCTISI 890
MG + ISI
Sbjct: 373 MGCIHGIISI 402
Score = 157 bits (337), Expect(2) = e-114
Identities = 68/89 (76%), Positives = 70/89 (78%)
Frame = +1 / +2
Query: 889 YTDNAMFVRDYTIKLLGTSFPNMTVAEVTKFVDGLLSSKLDLPSFKNHIRDFLVQSKEFS 1068
Y NA+FVRDYTIKLLG SFPNMTVAEVTKFVDGLL S+ DLPSFKNHIRDFLVQSKEFS
Sbjct: 395 YPSNALFVRDYTIKLLGASFPNMTVAEVTKFVDGLLGSRHDLPSFKNHIRDFLVQSKEFS 574
Query: 1069 AQDNKDLYXXXXXXXXXXXXXXMLAIPGL 1155
AQDNKDLY MLAIPGL
Sbjct: 575 AQDNKDLYAEEAAVQREKERQRMLAIPGL 661
Score = 141 bits (303), Expect(2) = 1e-060
Identities = 76/128 (59%), Positives = 84/128 (65%)
Frame = -3 / -2
Query: 889 IDMVQKTRPIEAPLDHQPHKTSVVAPKHEA*NQVYGMCL*GLQKSLAQLSKSRFCRIGFG 710
+DM+ PIE L QP KTSV APKHE * QVY M L* LQKS A SK+ CR
Sbjct: 401 MDMIPWMHPIEVQLGCQPRKTSVEAPKHEV*IQVYEMNL*ELQKSPALYSKNMSCRTDS* 222
Query: 709 ILKPGIFSRSPTTG*GQSLQYFSLYV*TPN*LNRSQASIADLKAGLMLGSNGCQLHEAKK 530
ILKP S PT *G+S Q FSLYV* P+* N+SQASIA KA L L SN C+ HE K+
Sbjct: 221 ILKPENSSEFPTID*GRSRQCFSLYV*MPS*SNQSQASIAGWKAELKL*SNECR*HEVKR 42
Query: 529 KI*GGAQD 506
K *GGAQD
Sbjct: 41 KT*GGAQD 18
Score = 133 bits (286), Expect(3) = 2e-047
Identities = 61/114 (53%), Positives = 78/114 (68%)
Frame = -1 / -3
Query: 843 INRTKQVL*HRNMKLETRFMECVCEDCKNLLLNCQKVGFVELVLES*SLEFFQDLQQQAK 664
+N KQVL H+NMK E+RFM+ +CE+CKNLLL QK+ VEL+ E *SL+ Q+ QQ K
Sbjct: 355 VNHAKQVLKHQNMKFESRFMK*ICENCKNLLLYIQKICLVELIREF*SLKILQNFQQ*TK 176
Query: 663 ASLCNISLCMSERPIN*IDHKXXXXXXXXXXXLEAMGANCTKQRKKFEAVLRII 502
A L N+SLCMS+ P+N I+HK EAM A+ TK R+K EAVLRI+
Sbjct: 175 AGLGNVSLCMSKCPVNRINHKLQLLAGKLN*SFEAMSADSTK*REKLEAVLRIL 14
Score = 124 bits (266), Expect(2) = 6e-087
Identities = 58/90 (64%), Positives = 60/90 (66%)
Frame = -2 / -1
Query: 1157 ISPGMASIXXXXXXXXXXXXXXXXXXXXXAENSFDCTRKSLIWFLKLGRSSFELRRPSTN 978
ISPGMASI AENSFDCTRKSLIWFLKLGRS E R PSTN
Sbjct: 663 ISPGMASIRCLSFSLCTAASSAYRSLLSWAENSFDCTRKSLIWFLKLGRSCLEPRSPSTN 484
Query: 977 LVTSATVMLGNDVPKSFMV*SRTNMALSVY 888
LVTSATVMLGND P+S +V*SRTN AL Y
Sbjct: 483 LVTSATVMLGNDAPRSLIV*SRTNKALDGY 394
Score = 113 bits (241), Expect(2) = 1e-060
Identities = 53/86 (61%), Positives = 55/86 (63%)
Frame = -3 / -2
Query: 1156 SAPEWQAFVGAPFLFGQQPLPRIDPCYPELRTPSTAPGNL*YGF*SSEDQVLN*EGHPQT 977
SA WQA V PFLF QPLP DPCYP LRTP APG+ *YGF*S V N H QT
Sbjct: 662 SALGWQASVAFPFLFVPQPLPHTDPCYPGLRTPLIAPGSP*YGF*SLGGHVSNQGAHQQT 483
Query: 976 WSPQLQSC*EMMFLRVLWCNLEQTWH 899
WSPQL SC*EMM V C+ QT H
Sbjct: 482 WSPQLPSC*EMMPQEV*LCSPVQTRH 405
Score = 111 bits (238), Expect(2) = 1e-069
Identities = 54/90 (60%), Positives = 64/90 (71%)
Frame = +2 / +3
Query: 887 NIRIMPCLFEITP*NS*EHHFLT*L*LR*PSLWMAFLVQNLIFRASKTILEISWCSRRSS 1066
+I +MPCL+ T N HHFLT* *LR*PSL M LV+++ +ASKTIL SWC++RSS
Sbjct: 393 HIHLMPCLYGTTQSNFLGHHFLT*R*LR*PSLLMGSLVRDMTSQASKTILGTSWCNQRSS 572
Query: 1067 QLRITRIYTRKRLLPKEKGSANECLPFRG* 1156
Q RITRI RKRL KEK N CLP +G*
Sbjct: 573 QPRITRICMRKRLRYKEKRKGNGCLPSQG* 662
Score = 109 bits (233), Expect(3) = 8e-049
Identities = 51/94 (54%), Positives = 64/94 (68%)
Frame = +2 / +3
Query: 611 IQLIGRSDIQREILQRLALACCWRS*KNSRLQDSKTNSTKPTF*QLSKRFLQSSQTHSIN 790
I+L G DIQRE L R AL CW+ *+ RLQ+S+ +ST+ F* S+RFLQ SQ H IN
Sbjct: 123 IRLTGHLDIQRETLPRPALVYCWKF*RIFRLQNSRISSTRHIF*I*SRRFLQFSQIHFIN 302
Query: 791 LVSSFMFRCYNTCFVRLMV*RSLYGTRLLYHINI 892
L S+FMF C+NTCF L * +LYG YHI++
Sbjct: 303 LDSNFMFWCFNTCFAWLTA*LNLYGMHPRYHIHL 404
Score = 99.1 bits (210), Expect(3) = 8e-049
Identities = 47/87 (54%), Positives = 55/87 (63%)
Frame = +3 / +1
Query: 897 *CHVCSRLHHKTLRNIIS*HDCS*GDQVCGWPS*FKT*SSELQKPY*RFPGAVEGVLXXX 1076
*C VC+ LH++T IIS*HD S*GDQVC W F+T* +LQKPY* PGA++GVL
Sbjct: 403 *CLVCTGLHNQTSWGIIS*HDGS*GDQVC*WAPWFET*PPKLQKPY*GLPGAIKGVLSPG 582
Query: 1077 XXXXXXXXXCCPKRKGAPTNACHSGAD 1157
C KRKG T+ACH AD
Sbjct: 583 *QGSVCGRGCGTKRKGKATDACHPRAD 663
Score = 68.4 bits (143), Expect(3) = 2e-047
Identities = 30/51 (58%), Positives = 37/51 (72%)
Frame = -1 / -3
Query: 1050 HQEISNMVFEARKIKF*TKKAIHKLGHLSYSHVRK*CS*EFYGVISNKHGI 898
HQE+ NMVFEA ++ TK+ I+KLGHLSY HVRK*C +F V+ K GI
Sbjct: 556 HQEVPNMVFEAWEVMSRTKEPINKLGHLSYRHVRK*CPKKFDCVVPYKQGI 404
Score = 26.7 bits (52), Expect(3) = 2e-047
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = -1 / -3
Query: 1155 QPRNGKHSLALPFSLGSSLF 1096
QP +GKH L FSL SLF
Sbjct: 661 QPWDGKHPLPFLFSLYRSLF 602
Score = 24.9 bits (48), Expect(3) = 8e-049
Identities = 13/19 (68%), Positives = 14/19 (73%)
Frame = +2 / +3
Query: 512 STASNFFLCFVQLAPIASK 568
STAS+F L FV A IASK
Sbjct: 24 STASSFSLYFVLSALIASK 80
>gb|BJ470140.1|BJ470140 BJ470140 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal15i11 5', mRNA sequence
Length = 643
Score = 276 bits (597), Expect(2) = e-111
Identities = 115/126 (91%), Positives = 118/126 (93%)
Frame = +1 / +2
Query: 508 PEHRLKFFSLLRAIGTHCFQALIQLSSQQLKLVIDSINWAFRHTERNIAETGLSLLLEIL 687
PEHRLKFFSLLRAIGTHCF+ALIQLSSQQLKLVIDSINWAFRHTERNIAETGLSLLLEIL
Sbjct: 14 PEHRLKFFSLLRAIGTHCFKALIQLSSQQLKLVIDSINWAFRHTERNIAETGLSLLLEIL 193
Query: 688 KKFQASGFQNQFYKTYFLTIEQEIFAVLTDTFHKPGFKLHVSVLQHLFCAVDGLTEPLWD 867
K FQAS F NQFYKTYFL IEQEIFAVLTD+FHKPGFKLHV VLQHLFC VD LTEPLWD
Sbjct: 194 KNFQASEFTNQFYKTYFLNIEQEIFAVLTDSFHKPGFKLHVLVLQHLFCVVDSLTEPLWD 373
Query: 868 ASSVPY 885
AS+V Y
Sbjct: 374 ASTVSY 391
Score = 217 bits (469), Expect(3) = 2e-084
Identities = 104/130 (80%), Positives = 108/130 (83%)
Frame = -2 / -2
Query: 884 YGTEDASHRGSVRPSTAQNKCCSTET*SLKPGLWNVSVRTAKISCSIVKK*VL*NWFWNP 705
Y T DASHRGSVR ST QNKC ST+T*SL PGL N SVRTAKISCSI KK VL*N F N
Sbjct: 390 YDTVDASHRGSVRLSTTQNKC*STKT*SLNPGL*NESVRTAKISCSIFKKYVL*N*FVNS 211
Query: 704 EAWNFFKISNNRLRPVSAIFLSVCLNAQLIESITSFNC*LESWINAWKQWVPIARSKEKN 525
EA FF+ISNNRLRPVSA+FLSVCLNAQLIESITSFNC LES I A KQ VPIARSKEKN
Sbjct: 210 EA*KFFRISNNRLRPVSAMFLSVCLNAQLIESITSFNCWLES*IKALKQ*VPIARSKEKN 31
Query: 524 LRRCSG*SSK 495
LRRCSG S +
Sbjct: 30 LRRCSGSSCR 1
Score = 172 bits (371), Expect(2) = 8e-068
Identities = 78/130 (60%), Positives = 93/130 (71%)
Frame = +3 / +1
Query: 501 RLS*APPQIFFFASCNWHPLLPSINPAFKSAIEACDRFN*LGVQTYREKYCRDWP*PVVG 680
R S*APPQ+F F SC H LL S N AF+ AIEACD F+*LG+ TYREK+CRD P* +VG
Sbjct: 7 RGS*APPQVFLFTSCYRHSLLQSFNSAFQPAIEACD*FD*LGI*TYREKHCRDRP*SIVG 186
Query: 681 DLEKIPGFRIPKPILQNLLFDN*ARDFCSPHRHIP*TWFQASCFGATTLVLCG*WSNGAS 860
+ E+ GFRI + +LQ++ F+ A DFCS HR I *TW Q SCFGA+TLVL G* N S
Sbjct: 187 NSEEFSGFRIHESVLQDIFFEYRAGDFCSSHRFIS*TWIQTSCFGASTLVLRG*QPN*TS 366
Query: 861 MGRVFCTISI 890
MG + ISI
Sbjct: 367 MGCIHGIISI 396
Score = 147 bits (316), Expect(2) = e-111
Identities = 64/85 (75%), Positives = 66/85 (77%)
Frame = +1 / +2
Query: 889 YTDNAMFVRDYTIKLLGTSFPNMTVAEVTKFVDGLLSSKLDLPSFKNHIRDFLVQSKEFS 1068
Y NA+FVRDYTIKLLG SFPNMTVAEVTKFVDGLL S+ DLPSFKNHIRDFLVQSKEFS
Sbjct: 389 YPSNALFVRDYTIKLLGASFPNMTVAEVTKFVDGLLGSRHDLPSFKNHIRDFLVQSKEFS 568
Query: 1069 AQDNKDLYXXXXXXXXXXXXXXMLA 1143
AQDNKDLY MLA
Sbjct: 569 AQDNKDLYAEEAAVQREKERQRMLA 643
Score = 141 bits (303), Expect(2) = 9e-059
Identities = 76/128 (59%), Positives = 84/128 (65%)
Frame = -3 / -3
Query: 889 IDMVQKTRPIEAPLDHQPHKTSVVAPKHEA*NQVYGMCL*GLQKSLAQLSKSRFCRIGFG 710
+DM+ PIE L QP KTSV APKHE * QVY M L* LQKS A SK+ CR
Sbjct: 395 MDMIPWMHPIEVQLGCQPRKTSVEAPKHEV*IQVYEMNL*ELQKSPALYSKNMSCRTDS* 216
Query: 709 ILKPGIFSRSPTTG*GQSLQYFSLYV*TPN*LNRSQASIADLKAGLMLGSNGCQLHEAKK 530
ILKP S PT *G+S Q FSLYV* P+* N+SQASIA KA L L SN C+ HE K+
Sbjct: 215 ILKPENSSEFPTID*GRSRQCFSLYV*MPS*SNQSQASIAGWKAELKL*SNECR*HEVKR 36
Query: 529 KI*GGAQD 506
K *GGAQD
Sbjct: 35 KT*GGAQD 12
Score = 133 bits (286), Expect(2) = 6e-045
Identities = 61/114 (53%), Positives = 78/114 (68%)
Frame = -1 / -1
Query: 843 INRTKQVL*HRNMKLETRFMECVCEDCKNLLLNCQKVGFVELVLES*SLEFFQDLQQQAK 664
+N KQVL H+NMK E+RFM+ +CE+CKNLLL QK+ VEL+ E *SL+ Q+ QQ K
Sbjct: 349 VNHAKQVLKHQNMKFESRFMK*ICENCKNLLLYIQKICLVELIREF*SLKILQNFQQ*TK 170
Query: 663 ASLCNISLCMSERPIN*IDHKXXXXXXXXXXXLEAMGANCTKQRKKFEAVLRII 502
A L N+SLCMS+ P+N I+HK EAM A+ TK R+K EAVLRI+
Sbjct: 169 AGLGNVSLCMSKCPVNRINHKLQLLAGKLN*SFEAMSADSTK*REKLEAVLRIL 8
Score = 113 bits (242), Expect(3) = 2e-084
Identities = 49/60 (81%), Positives = 51/60 (85%)
Frame = -2 / -2
Query: 1067 ENSFDCTRKSLIWFLKLGRSSFELRRPSTNLVTSATVMLGNDVPKSFMV*SRTNMALSVY 888
ENSFDCTRKSLIWFLKLGRS E R PSTNLVTSATVMLGND P+S +V*SRTN AL Y
Sbjct: 567 ENSFDCTRKSLIWFLKLGRSCLEPRSPSTNLVTSATVMLGNDAPRSLIV*SRTNKALDGY 388
Score = 109 bits (233), Expect(3) = 9e-047
Identities = 51/94 (54%), Positives = 64/94 (68%)
Frame = +2 / +3
Query: 611 IQLIGRSDIQREILQRLALACCWRS*KNSRLQDSKTNSTKPTF*QLSKRFLQSSQTHSIN 790
I+L G DIQRE L R AL CW+ *+ RLQ+S+ +ST+ F* S+RFLQ SQ H IN
Sbjct: 117 IRLTGHLDIQRETLPRPALVYCWKF*RIFRLQNSRISSTRHIF*I*SRRFLQFSQIHFIN 296
Query: 791 LVSSFMFRCYNTCFVRLMV*RSLYGTRLLYHINI 892
L S+FMF C+NTCF L * +LYG YHI++
Sbjct: 297 LDSNFMFWCFNTCFAWLTA*LNLYGMHPRYHIHL 398
Score = 106 bits (227), Expect(2) = 9e-059
Identities = 50/81 (61%), Positives = 52/81 (64%)
Frame = -3 / -3
Query: 1141 QAFVGAPFLFGQQPLPRIDPCYPELRTPSTAPGNL*YGF*SSEDQVLN*EGHPQTWSPQL 962
QA V PFLF QPLP DPCYP LRTP APG+ *YGF*S V N H QTWSPQL
Sbjct: 641 QASVAFPFLFVPQPLPHTDPCYPGLRTPLIAPGSP*YGF*SLGGHVSNQGAHQQTWSPQL 462
Query: 961 QSC*EMMFLRVLWCNLEQTWH 899
SC*EMM V C+ QT H
Sbjct: 461 PSC*EMMPQEV*LCSPVQTRH 399
Score = 105 bits (225), Expect(2) = 8e-068
Identities = 51/85 (60%), Positives = 60/85 (70%)
Frame = +2 / +3
Query: 887 NIRIMPCLFEITP*NS*EHHFLT*L*LR*PSLWMAFLVQNLIFRASKTILEISWCSRRSS 1066
+I +MPCL+ T N HHFLT* *LR*PSL M LV+++ +ASKTIL SWC++RSS
Sbjct: 387 HIHLMPCLYGTTQSNFLGHHFLT*R*LR*PSLLMGSLVRDMTSQASKTILGTSWCNQRSS 566
Query: 1067 QLRITRIYTRKRLLPKEKGSANECL 1141
Q RITRI RKRL KEK N CL
Sbjct: 567 QPRITRICMRKRLRYKEKRKGNGCL 641
Score = 92.3 bits (195), Expect(3) = 9e-047
Identities = 44/82 (53%), Positives = 52/82 (63%)
Frame = +3 / +1
Query: 897 *CHVCSRLHHKTLRNIIS*HDCS*GDQVCGWPS*FKT*SSELQKPY*RFPGAVEGVLXXX 1076
*C VC+ LH++T IIS*HD S*GDQVC W F+T* +LQKPY* PGA++GVL
Sbjct: 397 *CLVCTGLHNQTSWGIIS*HDGS*GDQVC*WAPWFET*PPKLQKPY*GLPGAIKGVLSPG 576
Query: 1077 XXXXXXXXXCCPKRKGAPTNAC 1142
C KRKG T+AC
Sbjct: 577 *QGSVCGRGCGTKRKGKATDAC 642
Score = 68.4 bits (143), Expect(2) = 6e-045
Identities = 30/51 (58%), Positives = 37/51 (72%)
Frame = -1 / -1
Query: 1050 HQEISNMVFEARKIKF*TKKAIHKLGHLSYSHVRK*CS*EFYGVISNKHGI 898
HQE+ NMVFEA ++ TK+ I+KLGHLSY HVRK*C +F V+ K GI
Sbjct: 550 HQEVPNMVFEAWEVMSRTKEPINKLGHLSYRHVRK*CPKKFDCVVPYKQGI 398
Score = 24.9 bits (48), Expect(3) = 9e-047
Identities = 13/19 (68%), Positives = 14/19 (73%)
Frame = +2 / +3
Query: 512 STASNFFLCFVQLAPIASK 568
STAS+F L FV A IASK
Sbjct: 18 STASSFSLYFVLSALIASK 74
Score = 22.1 bits (42), Expect(3) = 2e-084
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = -1 / -1
Query: 1143 GKHSLALPFSLGSSLF 1096
GKH L FSL SLF
Sbjct: 643 GKHPLPFLFSLYRSLF 596
>gb|AV934080.1|AV934080 AV934080 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baalp02 5', mRNA sequence
Length = 597
Score = 261 bits (564), Expect(3) = e-101
Identities = 109/121 (90%), Positives = 113/121 (93%)
Frame = +1 / +2
Query: 523 KFFSLLRAIGTHCFQALIQLSSQQLKLVIDSINWAFRHTERNIAETGLSLLLEILKKFQA 702
+FFSLLRAIGTHCF+ALIQLSSQQLKLVIDSINWAFRHTERNIAETGLSLLLEILK FQA
Sbjct: 8 QFFSLLRAIGTHCFKALIQLSSQQLKLVIDSINWAFRHTERNIAETGLSLLLEILKNFQA 187
Query: 703 SGFQNQFYKTYFLTIEQEIFAVLTDTFHKPGFKLHVSVLQHLFCAVDGLTEPLWDASSVP 882
S F NQFYKTYFL IEQEIFAVLTD+FHKPGFKLHV VLQHLFC VD LTEPLWDAS+V
Sbjct: 188 SEFTNQFYKTYFLNIEQEIFAVLTDSFHKPGFKLHVLVLQHLFCVVDSLTEPLWDASTVS 367
Query: 883 Y 885
Y
Sbjct: 368 Y 370
Score = 202 bits (435), Expect(3) = 7e-080
Identities = 97/120 (80%), Positives = 100/120 (83%)
Frame = -2 / -1
Query: 884 YGTEDASHRGSVRPSTAQNKCCSTET*SLKPGLWNVSVRTAKISCSIVKK*VL*NWFWNP 705
Y T DASHRGSVR ST QNKC ST+T*SL PGL N SVRTAKISCSI KK VL*N F N
Sbjct: 369 YDTVDASHRGSVRLSTTQNKC*STKT*SLNPGL*NESVRTAKISCSIFKKYVL*N*FVNS 190
Query: 704 EAWNFFKISNNRLRPVSAIFLSVCLNAQLIESITSFNC*LESWINAWKQWVPIARSKEKN 525
EA FF+ISNNRLRPVSA+FLSVCLNAQLIESITSFNC LES I A KQ VPIARSKEKN
Sbjct: 189 EA*KFFRISNNRLRPVSAMFLSVCLNAQLIESITSFNCWLES*IKALKQ*VPIARSKEKN 10
Score = 159 bits (342), Expect(3) = 2e-057
Identities = 71/122 (58%), Positives = 86/122 (70%)
Frame = +3 / +1
Query: 525 IFFFASCNWHPLLPSINPAFKSAIEACDRFN*LGVQTYREKYCRDWP*PVVGDLEKIPGF 704
+F F SC H LL S N AF+ AIEACD F+*LG+ TYREK+CRD P* +VG+ E+ GF
Sbjct: 10 VFLFTSCYRHSLLQSFNSAFQPAIEACD*FD*LGI*TYREKHCRDRP*SIVGNSEEFSGF 189
Query: 705 RIPKPILQNLLFDN*ARDFCSPHRHIP*TWFQASCFGATTLVLCG*WSNGASMGRVFCTI 884
RI + +LQ++ F+ A DFCS HR I *TW Q SCFGA+TLVL G* N SMG + I
Sbjct: 190 RIHESVLQDIFFEYRAGDFCSSHRFIS*TWIQTSCFGASTLVLRG*QPN*TSMGCIHGII 369
Query: 885 SI 890
SI
Sbjct: 370 SI 375
Score = 129 bits (276), Expect(3) = 8e-044
Identities = 70/121 (57%), Positives = 78/121 (64%)
Frame = -3 / -2
Query: 889 IDMVQKTRPIEAPLDHQPHKTSVVAPKHEA*NQVYGMCL*GLQKSLAQLSKSRFCRIGFG 710
+DM+ PIE L QP KTSV APKHE * QVY M L* LQKS A SK+ CR
Sbjct: 374 MDMIPWMHPIEVQLGCQPRKTSVEAPKHEV*IQVYEMNL*ELQKSPALYSKNMSCRTDS* 195
Query: 709 ILKPGIFSRSPTTG*GQSLQYFSLYV*TPN*LNRSQASIADLKAGLMLGSNGCQLHEAKK 530
ILKP S PT *G+S Q FSLYV* P+* N+SQASIA KA L L SN C+ HE K+
Sbjct: 194 ILKPENSSEFPTID*GRSRQCFSLYV*MPS*SNQSQASIAGWKAELKL*SNECR*HEVKR 15
Query: 529 K 527
K
Sbjct: 14 K 12
Score = 121 bits (258), Expect(2) = 4e-037
Identities = 55/106 (51%), Positives = 71/106 (66%)
Frame = -1 / -3
Query: 843 INRTKQVL*HRNMKLETRFMECVCEDCKNLLLNCQKVGFVELVLES*SLEFFQDLQQQAK 664
+N KQVL H+NMK E+RFM+ +CE+CKNLLL QK+ VEL+ E *SL+ Q+ QQ K
Sbjct: 328 VNHAKQVLKHQNMKFESRFMK*ICENCKNLLLYIQKICLVELIREF*SLKILQNFQQ*TK 149
Query: 663 ASLCNISLCMSERPIN*IDHKXXXXXXXXXXXLEAMGANCTKQRKK 526
A L N+SLCMS+ P+N I+HK EAM A+ TK R+K
Sbjct: 148 AGLGNVSLCMSKCPVNRINHKLQLLAGKLN*SFEAMSADSTK*REK 11
Score = 110 bits (234), Expect(3) = e-101
Identities = 45/52 (86%), Positives = 47/52 (90%)
Frame = +1 / +2
Query: 889 YTDNAMFVRDYTIKLLGTSFPNMTVAEVTKFVDGLLSSKLDLPSFKNHIRDF 1044
Y NA+FVRDYTIKLLG SFPNMTVAEVTKFVDGLL S+ DLPSFKNHIRDF
Sbjct: 368 YPSNALFVRDYTIKLLGASFPNMTVAEVTKFVDGLLGSRHDLPSFKNHIRDF 523
Score = 109 bits (233), Expect(2) = 4e-035
Identities = 51/94 (54%), Positives = 64/94 (68%)
Frame = +2 / +3
Query: 611 IQLIGRSDIQREILQRLALACCWRS*KNSRLQDSKTNSTKPTF*QLSKRFLQSSQTHSIN 790
I+L G DIQRE L R AL CW+ *+ RLQ+S+ +ST+ F* S+RFLQ SQ H IN
Sbjct: 96 IRLTGHLDIQRETLPRPALVYCWKF*RIFRLQNSRISSTRHIF*I*SRRFLQFSQIHFIN 275
Query: 791 LVSSFMFRCYNTCFVRLMV*RSLYGTRLLYHINI 892
L S+FMF C+NTCF L * +LYG YHI++
Sbjct: 276 LDSNFMFWCFNTCFAWLTA*LNLYGMHPRYHIHL 377
Score = 92.7 bits (196), Expect(3) = 7e-080
Identities = 41/52 (78%), Positives = 43/52 (82%)
Frame = -2 / -1
Query: 1043 KSLIWFLKLGRSSFELRRPSTNLVTSATVMLGNDVPKSFMV*SRTNMALSVY 888
KSLIWFLKLGRS E R PSTNLVTSATVMLGND P+S +V*SRTN AL Y
Sbjct: 522 KSLIWFLKLGRSCLEPRSPSTNLVTSATVMLGNDAPRSLIV*SRTNKALDGY 367
Score = 66.6 bits (139), Expect(3) = 2e-057
Identities = 31/50 (62%), Positives = 36/50 (72%)
Frame = +3 / +1
Query: 897 *CHVCSRLHHKTLRNIIS*HDCS*GDQVCGWPS*FKT*SSELQKPY*RFP 1046
*C VC+ LH++T IIS*HD S*GDQVC W F+T* +LQKPY* P
Sbjct: 376 *CLVCTGLHNQTSWGIIS*HDGS*GDQVC*WAPWFET*PPKLQKPY*GLP 525
Score = 62.9 bits (131), Expect(3) = 8e-044
Identities = 28/50 (56%), Positives = 36/50 (72%)
Frame = -1 / -3
Query: 1047 QEISNMVFEARKIKF*TKKAIHKLGHLSYSHVRK*CS*EFYGVISNKHGI 898
+E+ NMVFEA ++ TK+ I+KLGHLSY HVRK*C +F V+ K GI
Sbjct: 526 REVPNMVFEAWEVMSRTKEPINKLGHLSYRHVRK*CPKKFDCVVPYKQGI 377
Score = 59.7 bits (124), Expect(2) = 4e-035
Identities = 30/53 (56%), Positives = 37/53 (69%)
Frame = +2 / +3
Query: 887 NIRIMPCLFEITP*NS*EHHFLT*L*LR*PSLWMAFLVQNLIFRASKTILEIS 1045
+I +MPCL+ T N HHFLT* *LR*PSL M LV+++ +ASKTIL S
Sbjct: 366 HIHLMPCLYGTTQSNFLGHHFLT*R*LR*PSLLMGSLVRDMTSQASKTILGTS 524
Score = 55.1 bits (114), Expect(2) = 4e-037
Identities = 28/49 (57%), Positives = 30/49 (61%)
Frame = -3 / -2
Query: 1045 GNL*YGF*SSEDQVLN*EGHPQTWSPQLQSC*EMMFLRVLWCNLEQTWH 899
G+ *YGF*S V N H QTWSPQL SC*EMM V C+ QT H
Sbjct: 524 GSP*YGF*SLGGHVSNQGAHQQTWSPQLPSC*EMMPQEV*LCSPVQTRH 378
Score = 43.2 bits (88), Expect(3) = 7e-080
Identities = 15/22 (68%), Positives = 17/22 (77%)
Frame = -3 / -1
Query: 1108 QQPLPRIDPCYPELRTPSTAPG 1043
++PLP DPCYP LRTP APG
Sbjct: 588 RRPLPHTDPCYPGLRTPLIAPG 523
Score = 39.6 bits (80), Expect(3) = e-101
Identities = 16/16 (100%), Positives = 16/16 (100%)
Frame = +1 / +3
Query: 1045 LVQSKEFSAQDNKDLY 1092
LVQSKEFSAQDNKDLY
Sbjct: 525 LVQSKEFSAQDNKDLY 572
Score = 36.3 bits (73), Expect(3) = 2e-057
Identities = 14/21 (66%), Positives = 17/21 (80%)
Frame = +2 / +1
Query: 1040 ISWCSRRSSQLRITRIYTRKR 1102
+ WC++RSSQ RITRI RKR
Sbjct: 520 LPWCNQRSSQPRITRICMRKR 582
Score = 24.4 bits (47), Expect(3) = 8e-044
Identities = 8/9 (88%), Positives = 9/9 (100%)
Frame = -2 / -3
Query: 1067 ENSFDCTRK 1041
ENSFDCTR+
Sbjct: 547 ENSFDCTRE 521
>gb|AV915093.1|AV915093 AV915093 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags9n09 5', mRNA sequence
Length = 532
Score = 332 bits (719), Expect(2) = e-100
Identities = 138/150 (92%), Positives = 141/150 (94%)
Frame = +1 / +2
Query: 436 DVPRIFEAVFQCTLEMITKNFEDYPEHRLKFFSLLRAIGTHCFQALIQLSSQQLKLVIDS 615
DVPRIFEAVFQCTL MITKNFEDYPEHRLKFFSLLRAIGTHCF+ALIQLSSQQLKLVIDS
Sbjct: 2 DVPRIFEAVFQCTLXMITKNFEDYPEHRLKFFSLLRAIGTHCFKALIQLSSQQLKLVIDS 181
Query: 616 INWAFRHTERNIAETGLSLLLEILKKFQASGFQNQFYKTYFLTIEQEIFAVLTDTFHKPG 795
INWAFRHTERNIAETGLSLLLEILK FQAS F NQFYKTYFL IEQEIFAVLTD+FHKPG
Sbjct: 182 INWAFRHTERNIAETGLSLLLEILKNFQASEFTNQFYKTYFLNIEQEIFAVLTDSFHKPG 361
Query: 796 FKLHVSVLQHLFCAVDGLTEPLWDASSVPY 885
FKLHV VLQHLFC VD LTEPLWDAS+V Y
Sbjct: 362 FKLHVLVLQHLFCVVDSLTEPLWDASTVSY 451
Score = 268 bits (579), Expect(2) = 7e-078
Identities = 126/150 (84%), Positives = 129/150 (86%)
Frame = -2 / -2
Query: 884 YGTEDASHRGSVRPSTAQNKCCSTET*SLKPGLWNVSVRTAKISCSIVKK*VL*NWFWNP 705
Y T DASHRGSVR ST QNKC ST+T*SL PGL N SVRTAKISCSI KK VL*N F N
Sbjct: 450 YDTVDASHRGSVRLSTTQNKC*STKT*SLNPGL*NESVRTAKISCSIFKKYVL*N*FVNS 271
Query: 704 EAWNFFKISNNRLRPVSAIFLSVCLNAQLIESITSFNC*LESWINAWKQWVPIARSKEKN 525
EA FF+ISNNRLRPVSA+FLSVCLNAQLIESITSFNC LES I A KQ VPIARSKEKN
Sbjct: 270 EA*KFFRISNNRLRPVSAMFLSVCLNAQLIESITSFNCWLES*IKALKQ*VPIARSKEKN 91
Query: 524 LRRCSG*SSKFLVIISRVHWKTASNIRGTS 435
LRRCSG*SSKFLVI SRVHWKTASNIRGTS
Sbjct: 90 LRRCSG*SSKFLVIXSRVHWKTASNIRGTS 1
Score = 215 bits (464), Expect(2) = 1e-058
Identities = 96/151 (63%), Positives = 113/151 (74%)
Frame = +3 / +1
Query: 438 CASYI*GCFPVHP*DDY*EL*RLS*APPQIFFFASCNWHPLLPSINPAFKSAIEACDRFN 617
CASYI GCFPV+P* DY*+L*RLS*APPQ+F F SC H LL S N AF+ AIEACD F+
Sbjct: 4 CASYIRGCFPVYP*XDY*KL*RLS*APPQVFLFTSCYRHSLLQSFNSAFQPAIEACD*FD 183
Query: 618 *LGVQTYREKYCRDWP*PVVGDLEKIPGFRIPKPILQNLLFDN*ARDFCSPHRHIP*TWF 797
*LG+ TYREK+CRD P* +VG+ E+ GFRI + +LQ++ F+ A DFCS HR I *TW
Sbjct: 184 *LGI*TYREKHCRDRP*SIVGNSEEFSGFRIHESVLQDIFFEYRAGDFCSSHRFIS*TWI 363
Query: 798 QASCFGATTLVLCG*WSNGASMGRVFCTISI 890
Q SCFGA+TLVL G* N SMG + ISI
Sbjct: 364 QTSCFGASTLVLRG*QPN*TSMGCIHGIISI 456
Score = 178 bits (384), Expect(2) = 1e-046
Identities = 80/136 (58%), Positives = 97/136 (71%)
Frame = -1 / -1
Query: 843 INRTKQVL*HRNMKLETRFMECVCEDCKNLLLNCQKVGFVELVLES*SLEFFQDLQQQAK 664
+N KQVL H+NMK E+RFM+ +CE+CKNLLL QK+ VEL+ E *SL+ Q+ QQ K
Sbjct: 409 VNHAKQVLKHQNMKFESRFMK*ICENCKNLLLYIQKICLVELIREF*SLKILQNFQQ*TK 230
Query: 663 ASLCNISLCMSERPIN*IDHKXXXXXXXXXXXLEAMGANCTKQRKKFEAVLRIIFKVLSN 484
A L N+SLCMS+ P+N I+HK EAM A+ TK R+K EAVLRIIFKV SN
Sbjct: 229 AGLGNVSLCMSKCPVNRINHKLQLLAGKLN*SFEAMSADSTK*REKLEAVLRIIFKVFSN 50
Query: 483 HLKGALENSLKYTRHI 436
H KG LENSL+YTRHI
Sbjct: 49 HXKGTLENSLEYTRHI 2
Score = 174 bits (374), Expect(2) = 1e-043
Identities = 91/151 (60%), Positives = 100/151 (66%)
Frame = -3 / -3
Query: 889 IDMVQKTRPIEAPLDHQPHKTSVVAPKHEA*NQVYGMCL*GLQKSLAQLSKSRFCRIGFG 710
+DM+ PIE L QP KTSV APKHE * QVY M L* LQKS A SK+ CR
Sbjct: 455 MDMIPWMHPIEVQLGCQPRKTSVEAPKHEV*IQVYEMNL*ELQKSPALYSKNMSCRTDS* 276
Query: 709 ILKPGIFSRSPTTG*GQSLQYFSLYV*TPN*LNRSQASIADLKAGLMLGSNGCQLHEAKK 530
ILKP S PT *G+S Q FSLYV* P+* N+SQASIA KA L L SN C+ HE K+
Sbjct: 275 ILKPENSSEFPTID*GRSRQCFSLYV*MPS*SNQSQASIAGWKAELKL*SNECR*HEVKR 96
Query: 529 KI*GGAQDNLQXXXXXSQGCTGKQPQIYEAH 437
K *GGAQDNLQ QG TGKQP+IYEAH
Sbjct: 95 KT*GGAQDNLQSF**SXQGYTGKQPRIYEAH 3
Score = 109 bits (233), Expect(3) = 2e-034
Identities = 51/94 (54%), Positives = 64/94 (68%)
Frame = +2 / +3
Query: 611 IQLIGRSDIQREILQRLALACCWRS*KNSRLQDSKTNSTKPTF*QLSKRFLQSSQTHSIN 790
I+L G DIQRE L R AL CW+ *+ RLQ+S+ +ST+ F* S+RFLQ SQ H IN
Sbjct: 177 IRLTGHLDIQRETLPRPALVYCWKF*RIFRLQNSRISSTRHIF*I*SRRFLQFSQIHFIN 356
Query: 791 LVSSFMFRCYNTCFVRLMV*RSLYGTRLLYHINI 892
L S+FMF C+NTCF L * +LYG YHI++
Sbjct: 357 LDSNFMFWCFNTCFAWLTA*LNLYGMHPRYHIHL 458
Score = 55.6 bits (115), Expect(2) = e-100
Identities = 22/26 (84%), Positives = 23/26 (88%)
Frame = +1 / +2
Query: 889 YTDNAMFVRDYTIKLLGTSFPNMTVA 966
Y NA+FVRDYTIKLLG SFPNMTVA
Sbjct: 449 YPSNALFVRDYTIKLLGASFPNMTVA 526
Score = 44.6 bits (91), Expect(3) = 2e-034
Identities = 25/44 (56%), Positives = 26/44 (59%)
Frame = +2 / +3
Query: 437 MCLVYLRLFSSAPXXXXXXXXXXXXSTASNFFLCFVQLAPIASK 568
MCLVY RLFSS P STAS+F L FV A IASK
Sbjct: 3 MCLVYSRLFSSVPLX*LLKTLKIILSTASSFSLYFVLSALIASK 134
Score = 44.1 bits (90), Expect(2) = 7e-078
Identities = 19/26 (73%), Positives = 21/26 (80%)
Frame = -2 / -2
Query: 965 ATVMLGNDVPKSFMV*SRTNMALSVY 888
ATVMLGND P+S +V*SRTN AL Y
Sbjct: 525 ATVMLGNDAPRSLIV*SRTNKALDGY 448
Score = 32.7 bits (65), Expect(2) = 1e-058
Identities = 15/27 (55%), Positives = 18/27 (66%)
Frame = +2 / +3
Query: 887 NIRIMPCLFEITP*NS*EHHFLT*L*L 967
+I +MPCL+ T N HHFLT* *L
Sbjct: 447 HIHLMPCLYGTTQSNFLGHHFLT*R*L 527
Score = 30.9 bits (61), Expect(3) = 2e-034
Identities = 14/23 (60%), Positives = 17/23 (73%)
Frame = +3 / +1
Query: 897 *CHVCSRLHHKTLRNIIS*HDCS 965
*C VC+ LH++T IIS*HD S
Sbjct: 457 *CLVCTGLHNQTSWGIIS*HDGS 525
Score = 29.0 bits (57), Expect(2) = 1e-046
Identities = 13/23 (56%), Positives = 15/23 (65%)
Frame = -1 / -1
Query: 966 SYSHVRK*CS*EFYGVISNKHGI 898
SY HVRK*C +F V+ K GI
Sbjct: 526 SYRHVRK*CPKKFDCVVPYKQGI 458
Score = 23.5 bits (45), Expect(2) = 1e-043
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = -3 / -3
Query: 967 QLQSC*EMMFLRVLWCNLEQTWH 899
+L SC*EMM V C+ QT H
Sbjct: 527 KLPSC*EMMPQEV*LCSPVQTRH 459
>gb|BJ473588.1|BJ473588 BJ473588 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal15i11 3', mRNA sequence
Length = 736
Score = 178 bits (383), Expect(2) = 8e-045
Identities = 79/97 (81%), Positives = 80/97 (82%)
Frame = +1 / -2
Query: 907 FVRDYTIKLLGTSFPNMTVAEVTKFVDGLLSSKLDLPSFKNHIRDFLVQSKEFSAQDNKD 1086
FVRDYTIKLLG SFPNMTVAEVTKFVDGLL S+ DLPSFKNHIRDFLVQSKEFSAQDNKD
Sbjct: 735 FVRDYTIKLLGASFPNMTVAEVTKFVDGLLGSRHDLPSFKNHIRDFLVQSKEFSAQDNKD 556
Query: 1087 LYXXXXXXXXXXXXXXMLAIPGLIAPSELQDEMVDS* 1197
LY MLAIPGLIAPSELQDEMVDS*
Sbjct: 555 LYAEEAAVQREKERQRMLAIPGLIAPSELQDEMVDS* 445
Score = 155 bits (334), Expect = 5e-037
Identities = 70/99 (70%), Positives = 72/99 (72%)
Frame = -2 / +2
Query: 1202 EHYESTISSCNSLGAISPGMASIXXXXXXXXXXXXXXXXXXXXXAENSFDCTRKSLIWFL 1023
EHYESTISSCNSLGAISPGMASI AENSFDCTRKSLIWFL
Sbjct: 440 EHYESTISSCNSLGAISPGMASIRCLSFSLCTAASSAYRSLLSWAENSFDCTRKSLIWFL 619
Query: 1022 KLGRSSFELRRPSTNLVTSATVMLGNDVPKSFMV*SRTN 906
KLGRS E R PSTNLVTSATVMLGND P+S +V*SRTN
Sbjct: 620 KLGRSCLEPRSPSTNLVTSATVMLGNDAPRSLIV*SRTN 736
Score = 136 bits (292), Expect = 3e-031
Identities = 63/101 (62%), Positives = 70/101 (69%)
Frame = -3 / +3
Query: 1219 SRIKTRNTTNLPSRLAIR*GQSAPEWQAFVGAPFLFGQQPLPRIDPCYPELRTPSTAPGN 1040
S ++++T +LPSRLAIR*GQSA WQA V PFLF QPLP DPCYP LRTP APG+
Sbjct: 423 SNAESQSTMSLPSRLAIR*GQSALGWQASVAFPFLFVPQPLPHTDPCYPGLRTPLIAPGS 602
Query: 1039 L*YGF*SSEDQVLN*EGHPQTWSPQLQSC*EMMFLRVLWCN 917
*YGF*S V N H QTWSPQL SC*EMM V C+
Sbjct: 603 P*YGF*SLGGHVSNQGAHQQTWSPQLPSC*EMMPQEV*LCS 725
Score = 128 bits (274), Expect = 9e-029
Identities = 62/97 (63%), Positives = 70/97 (72%)
Frame = +2 / -3
Query: 908 LFEITP*NS*EHHFLT*L*LR*PSLWMAFLVQNLIFRASKTILEISWCSRRSSQLRITRI 1087
L+ T N HHFLT* *LR*PSL M LV+++ +ASKTIL SWC++RSSQ RITRI
Sbjct: 734 LYGTTQSNFLGHHFLT*R*LR*PSLLMGSLVRDMTSQASKTILGTSWCNQRSSQPRITRI 555
Query: 1088 YTRKRLLPKEKGSANECLPFRG*LPLANCKTRW*IRS 1198
RKRL KEK N CLP +G*LPLANCKTRW* S
Sbjct: 554 CMRKRLRYKEKRKGNGCLPSQG*LPLANCKTRW*THS 444
Score = 122 bits (260), Expect = 7e-027
Identities = 56/95 (58%), Positives = 64/95 (67%)
Frame = +3 / -1
Query: 906 VCSRLHHKTLRNIIS*HDCS*GDQVCGWPS*FKT*SSELQKPY*RFPGAVEGVLXXXXXX 1085
VC+ LH++T IIS*HD S*GDQVC W F+T* +LQKPY* PGA++GVL
Sbjct: 736 VCTGLHNQTSWGIIS*HDGS*GDQVC*WAPWFET*PPKLQKPY*GLPGAIKGVLSPG*QG 557
Query: 1086 XXXXXXCCPKRKGAPTNACHSGADCP*RIARRDGR 1190
C KRKG T+ACH ADCP*RIARRDGR
Sbjct: 556 SVCGRGCGTKRKGKATDACHPRADCP*RIARRDGR 452
Score = 23.5 bits (45), Expect(2) = 8e-045
Identities = 7/10 (70%), Positives = 8/10 (80%)
Frame = +2 / -3
Query: 1301 KGGCCNFIFG 1330
+G CCNFI G
Sbjct: 338 EGACCNFISG 309
>gb|AV833703.1|AV833703 AV833703 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags12e10, mRNA sequence
Length = 211
Score = 157 bits (338), Expect = 1e-037
Identities = 64/70 (91%), Positives = 68/70 (97%)
Frame = +1 / +2
Query: 247 TLKLIETFVDKAEDLPHVGKQFVPPMMDPVLGDYARNVPDARESEVLSLFATIINKYKGE 426
TLKLIETFVDKAEDLPH+GKQFVPPMMDP+LGDYARNVPDARESEVLSLFAT+INK K E
Sbjct: 2 TLKLIETFVDKAEDLPHLGKQFVPPMMDPILGDYARNVPDARESEVLSLFATVINKXKAE 181
Query: 427 MLEDVPRIFE 456
ML+DVPRIFE
Sbjct: 182 MLDDVPRIFE 211
Score = 136 bits (292), Expect = 3e-031
Identities = 60/70 (85%), Positives = 63/70 (90%)
Frame = -2 / -2
Query: 455 SNIRGTSSSISPLYLLMIVANKDRTSDSLASGTFLA*SPRTGSIIGGTNCFPTCGKSSAL 276
SNIRGTSSSIS L L + VAN+DRTSDSLASGTFLA*SPR GSIIGGTNCFP CG+SSAL
Sbjct: 210 SNIRGTSSSISALXLFITVANRDRTSDSLASGTFLA*SPRIGSIIGGTNCFPRCGRSSAL 31
Query: 275 STKVSINFRV 246
STKVSINF V
Sbjct: 30 STKVSINFNV 1
Score = 120 bits (256), Expect = 3e-026
Identities = 46/67 (68%), Positives = 54/67 (80%)
Frame = -1 / -1
Query: 456 LKYTRHILKHFPFVLVNDCCKQGQNFRLPCIGYISSIITKNRIHHWWYKLLSYMWQIFRL 277
L+YTRHI+KHF F LV +CCKQGQ+FR PCI +IS IITKNRIHHW KLL +W +F
Sbjct: 211 LEYTRHIIKHFSFXLVYNCCKQGQDFRFPCIRHISCIITKNRIHHWRNKLLPKVW*VFSF 32
Query: 276 VHKSLDQ 256
VHKSL+Q
Sbjct: 31 VHKSLNQ 11
>gb|BI779104.2|BI779104 EBro01_SQ003_A04_R root, 3 week, hydroponic grown, no treatment, cv
Optic, EBro01 Hordeum vulgare subsp. vulgare cDNA clone
EBro01_SQ003_A04 5', mRNA sequence
Length = 638
Score = 152 bits (326), Expect(2) = 5e-037
Identities = 68/85 (80%), Positives = 69/85 (81%)
Frame = +1 / +2
Query: 943 SFPNMTVAEVTKFVDGLLSSKLDLPSFKNHIRDFLVQSKEFSAQDNKDLYXXXXXXXXXX 1122
SFPNMTVAEVTKFVDGLL S+ DLPSFKNHIRDFLVQSKEFSAQDNKDLY
Sbjct: 2 SFPNMTVAEVTKFVDGLLGSRHDLPSFKNHIRDFLVQSKEFSAQDNKDLYGEEAAVQREK 181
Query: 1123 XXXXMLAIPGLIAPSELQDEMVDS* 1197
MLAIPGLIAPSELQDEMVDS*
Sbjct: 182 ERQRMLAIPGLIAPSELQDEMVDS* 256
Score = 133 bits (285), Expect = 3e-030
Identities = 60/92 (65%), Positives = 66/92 (71%)
Frame = -3 / -1
Query: 1219 SRIKTRNTTNLPSRLAIR*GQSAPEWQAFVGAPFLFGQQPLPRIDPCYPELRTPSTAPGN 1040
S ++++T +LPSRLAIR*GQSA WQA V PFLF QPLP DPCYP LRTP APG+
Sbjct: 278 SNAESQSTMSLPSRLAIR*GQSALGWQASVAFPFLFVPQPLPHTDPCYPGLRTPLIAPGS 99
Query: 1039 L*YGF*SSEDQVLN*EGHPQTWSPQLQSC*EM 944
*YGF*S V N H QTWSPQL SC*EM
Sbjct: 98 P*YGF*SLGGHVSNQGAHQQTWSPQLPSC*EM 3
Score = 120 bits (257), Expect = 2e-026
Identities = 57/85 (67%), Positives = 64/85 (75%)
Frame = +2 / +3
Query: 944 HFLT*L*LR*PSLWMAFLVQNLIFRASKTILEISWCSRRSSQLRITRIYTRKRLLPKEKG 1123
HFLT* *LR*PSL M LV+++ +ASKTIL SWC++RSSQ RITRI KRL KEK
Sbjct: 3 HFLT*R*LR*PSLLMGSLVRDMTSQASKTILGTSWCNQRSSQPRITRICMGKRLRYKEKR 182
Query: 1124 SANECLPFRG*LPLANCKTRW*IRS 1198
N CLP +G*LPLANCKTRW* S
Sbjct: 183 KGNGCLPSQG*LPLANCKTRW*THS 257
Score = 110 bits (234), Expect = 3e-023
Identities = 51/83 (61%), Positives = 56/83 (67%)
Frame = +3 / +1
Query: 942 IIS*HDCS*GDQVCGWPS*FKT*SSELQKPY*RFPGAVEGVLXXXXXXXXXXXXCCPKRK 1121
IIS*HD S*GDQVC W F+T* +LQKPY* PGA++GVL C KRK
Sbjct: 1 IIS*HDGS*GDQVC*WAPWFET*PPKLQKPY*GLPGAIKGVLSPG*QGSVWGRGCGTKRK 180
Query: 1122 GAPTNACHSGADCP*RIARRDGR 1190
G T+ACH ADCP*RIARRDGR
Sbjct: 181 GKATDACHPRADCP*RIARRDGR 249
Score = 90.9 bits (192), Expect(2) = 1e-028
Identities = 38/42 (90%), Positives = 38/42 (90%)
Frame = -2 / -3
Query: 1067 ENSFDCTRKSLIWFLKLGRSSFELRRPSTNLVTSATVMLGND 942
ENSFDCTRKSLIWFLKLGRS E R PSTNLVTSATVMLGND
Sbjct: 126 ENSFDCTRKSLIWFLKLGRSCLEPRSPSTNLVTSATVMLGND 1
Score = 56.5 bits (117), Expect(2) = 1e-028
Identities = 23/23 (100%), Positives = 23/23 (100%)
Frame = -2 / -3
Query: 1202 EHYESTISSCNSLGAISPGMASI 1134
EHYESTISSCNSLGAISPGMASI
Sbjct: 261 EHYESTISSCNSLGAISPGMASI 193
Score = 23.5 bits (45), Expect(2) = 5e-037
Identities = 7/10 (70%), Positives = 8/10 (80%)
Frame = +2 / +3
Query: 1301 KGGCCNFIFG 1330
+G CCNFI G
Sbjct: 363 EGACCNFISG 392
>gb|AV920227.1|AV920227 AV920227 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags9n09 3', mRNA sequence
Length = 665
Score = 138 bits (295), Expect(2) = 8e-033
Identities = 64/87 (73%), Positives = 65/87 (74%)
Frame = +1 / -2
Query: 937 GTSFPNMTVAEVTKFVDGLLSSKLDLPSFKNHIRDFLVQSKEFSAQDNKDLYXXXXXXXX 1116
G F VAEVTKFVDGLL S+ DLPSFKNHIRDFLVQSKEFSAQDNKDLY
Sbjct: 664 GHHFLT*PVAEVTKFVDGLLGSRHDLPSFKNHIRDFLVQSKEFSAQDNKDLYAEEAAVQR 485
Query: 1117 XXXXXXMLAIPGLIAPSELQDEMVDS* 1197
MLAIPGLIAPSELQDEMVDS*
Sbjct: 484 EKERQRMLAIPGLIAPSELQDEMVDS* 404
Score = 123 bits (264), Expect = 2e-027
Identities = 56/81 (69%), Positives = 56/81 (69%)
Frame = -2 / +3
Query: 1202 EHYESTISSCNSLGAISPGMASIXXXXXXXXXXXXXXXXXXXXXAENSFDCTRKSLIWFL 1023
EHYESTISSCNSLGAISPGMASI AENSFDCTRKSLIWFL
Sbjct: 399 EHYESTISSCNSLGAISPGMASIRCLSFSLCTAASSAYRSLLSWAENSFDCTRKSLIWFL 578
Query: 1022 KLGRSSFELRRPSTNLVTSAT 960
KLGRS E R PSTNLVTSAT
Sbjct: 579 KLGRSCLEPRSPSTNLVTSAT 641
Score = 122 bits (262), Expect = 4e-027
Identities = 55/86 (63%), Positives = 61/86 (70%)
Frame = -3 / +1
Query: 1219 SRIKTRNTTNLPSRLAIR*GQSAPEWQAFVGAPFLFGQQPLPRIDPCYPELRTPSTAPGN 1040
S ++++T +LPSRLAIR*GQSA WQA V PFLF QPLP DPCYP LRTP APG+
Sbjct: 382 SNAESQSTMSLPSRLAIR*GQSALGWQASVAFPFLFVPQPLPHTDPCYPGLRTPLIAPGS 561
Query: 1039 L*YGF*SSEDQVLN*EGHPQTWSPQL 962
*YGF*S V N H QTWSPQL
Sbjct: 562 P*YGF*SLGGHVSNQGAHQQTWSPQL 639
Score = 113 bits (242), Expect = 2e-024
Identities = 53/79 (67%), Positives = 60/79 (75%)
Frame = +2 / -3
Query: 962 *LR*PSLWMAFLVQNLIFRASKTILEISWCSRRSSQLRITRIYTRKRLLPKEKGSANECL 1141
*LR*PSL M LV+++ +ASKTIL SWC++RSSQ RITRI RKRL KEK N CL
Sbjct: 639 *LR*PSLLMGSLVRDMTSQASKTILGTSWCNQRSSQPRITRICMRKRLRYKEKRKGNGCL 460
Query: 1142 PFRG*LPLANCKTRW*IRS 1198
P +G*LPLANCKTRW* S
Sbjct: 459 PSQG*LPLANCKTRW*THS 403
Score = 23.5 bits (45), Expect(2) = 8e-033
Identities = 7/10 (70%), Positives = 8/10 (80%)
Frame = +2 / -3
Query: 1301 KGGCCNFIFG 1330
+G CCNFI G
Sbjct: 297 EGACCNFISG 268
>gb|BJ474628.1|BJ474628 BJ474628 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal20e17 3', mRNA sequence
Length = 643
Score = 111 bits (237), Expect(2) = 7e-025
Identities = 50/66 (75%), Positives = 51/66 (77%)
Frame = +1 / -3
Query: 1000 SKLDLPSFKNHIRDFLVQSKEFSAQDNKDLYXXXXXXXXXXXXXXMLAIPGLIAPSELQD 1179
S+ DLPSFKNHIRDFLVQSKEFSAQDNKDLY MLAIPGLIAPSELQD
Sbjct: 641 SRHDLPSFKNHIRDFLVQSKEFSAQDNKDLYAEEAAVQREKERQRMLAIPGLIAPSELQD 462
Query: 1180 EMVDS* 1197
EMVDS*
Sbjct: 461 EMVDS* 444
Score = 103 bits (220), Expect = 2e-021
Identities = 47/74 (63%), Positives = 53/74 (71%)
Frame = -3 / +2
Query: 1219 SRIKTRNTTNLPSRLAIR*GQSAPEWQAFVGAPFLFGQQPLPRIDPCYPELRTPSTAPGN 1040
S ++++T +LPSRLAIR*GQSA WQA V PFLF QPLP DPCYP LRTP APG+
Sbjct: 422 SNAESQSTMSLPSRLAIR*GQSALGWQASVAFPFLFVPQPLPHTDPCYPGLRTPLIAPGS 601
Query: 1039 L*YGF*SSEDQVLN 998
*YGF*S V N
Sbjct: 602 P*YGF*SLGGHVSN 643
Score = 23.5 bits (45), Expect(2) = 7e-025
Identities = 7/10 (70%), Positives = 8/10 (80%)
Frame = +2 / -1
Query: 1301 KGGCCNFIFG 1330
+G CCNFI G
Sbjct: 337 EGACCNFISG 308
>gb|AV936330.1|AV936330 AV936330 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal10p02 3', mRNA sequence
Length = 613
Score = 111 bits (237), Expect(2) = 7e-025
Identities = 50/66 (75%), Positives = 51/66 (77%)
Frame = +1 / -3
Query: 1000 SKLDLPSFKNHIRDFLVQSKEFSAQDNKDLYXXXXXXXXXXXXXXMLAIPGLIAPSELQD 1179
S+ DLPSFKNHIRDFLVQSKEFSAQDNKDLY MLAIPGLIAPSELQD
Sbjct: 611 SRHDLPSFKNHIRDFLVQSKEFSAQDNKDLYAEEAAVQREKERQRMLAIPGLIAPSELQD 432
Query: 1180 EMVDS* 1197
EMVDS*
Sbjct: 431 EMVDS* 414
Score = 103 bits (220), Expect = 2e-021
Identities = 47/74 (63%), Positives = 53/74 (71%)
Frame = -3 / +2
Query: 1219 SRIKTRNTTNLPSRLAIR*GQSAPEWQAFVGAPFLFGQQPLPRIDPCYPELRTPSTAPGN 1040
S ++++T +LPSRLAIR*GQSA WQA V PFLF QPLP DPCYP LRTP APG+
Sbjct: 392 SNAESQSTMSLPSRLAIR*GQSALGWQASVAFPFLFVPQPLPHTDPCYPGLRTPLIAPGS 571
Query: 1039 L*YGF*SSEDQVLN 998
*YGF*S V N
Sbjct: 572 P*YGF*SLGGHVSN 613
Score = 23.5 bits (45), Expect(2) = 7e-025
Identities = 7/10 (70%), Positives = 8/10 (80%)
Frame = +2 / -2
Query: 1301 KGGCCNFIFG 1330
+G CCNFI G
Sbjct: 306 EGACCNFISG 277
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 739,653,313
Number of Sequences: 312970
Number of extensions: 11785792
Number of successful extensions: 61111
Number of sequences better than 1.0e-020: 17
length of database: 58,378,179
effective HSP length: 54
effective length of database: 41,477,799
effective search space used: 19826387922
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)