TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF1g01.yg.2.1
(335 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY177665.1| Hordeum vulgare subsp. vulgare putative call... 190 3e-048
gb|AV923029.1|AV923029 AV923029 K. Sato unpublished cDNA li... 170 3e-042
gb|BJ462458.1|BJ462458 BJ462458 K. Sato unpublished cDNA li... 167 2e-041
gb|BU969103.1|BU969103 HB10J09r BC Hordeum vulgare subsp. v... 137 2e-032
gb|AV836635.1|AV836635 AV836635 K. Sato unpublished cDNA li... 121 2e-027
gb|AV934137.1|AV934137 AV934137 K. Sato unpublished cDNA li... 120 3e-027
gb|BJ470174.1|BJ470174 BJ470174 K. Sato unpublished cDNA li... 120 3e-027
>gb|AY177665.1| Hordeum vulgare subsp. vulgare putative callose synthase mRNA,
complete cds
Length = 6180
Score = 190 bits (409), Expect = 3e-048
Identities = 79/88 (89%), Positives = 82/88 (93%)
Frame = -2 / +3
Query: 265 KVRXXYXXPDVFDRIFXITRGGIXKASRVINISEDIYAGFNSTLRQGNITHHEYIQVGKG 86
KVR Y PDVFDRIF ITRGGI KASR+INISEDI+AGFNSTLRQGNITHHEYIQVGKG
Sbjct: 4233 KVRMHYGHPDVFDRIFHITRGGISKASRIINISEDIFAGFNSTLRQGNITHHEYIQVGKG 4412
Query: 85 RDVGLNQIALFEGKVAGGNGEQVLSRDV 2
RDVGLNQIALFEGKVAGGNGEQVLSRD+
Sbjct: 4413 RDVGLNQIALFEGKVAGGNGEQVLSRDI 4496
Score = 135 bits (289), Expect = 1e-031
Identities = 59/79 (74%), Positives = 61/79 (77%)
Frame = +3 / -3
Query: 6 SRLRTCSPLPPATFPSNSAIWFKPTSRPFPTWIYSWCVMFPWRKVELNPAYISSLILITR 185
S LRTCSP PPATFPSNSAI PTS P PTWIYSW VMFP R+VELNPA ISSLIL+ R
Sbjct: 4492 SLLRTCSPFPPATFPSNSAI*LSPTSLPLPTWIYSW*VMFP*RRVELNPANISSLILMIR 4313
Query: 186 DAXXMPPRVXXNIRSKTSG 242
DA MPP V NI S TSG
Sbjct: 4312 DALLMPPLVI*NILSNTSG 4256
>gb|AV923029.1|AV923029 AV923029 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd3g22 5', mRNA sequence
Length = 611
Score = 170 bits (365), Expect = 3e-042
Identities = 64/88 (72%), Positives = 79/88 (89%)
Frame = -2 / +2
Query: 265 KVRXXYXXPDVFDRIFXITRGGIXKASRVINISEDIYAGFNSTLRQGNITHHEYIQVGKG 86
+VR Y PD+FDR+F +TRGG+ KAS++IN+SEDI+AGFNSTLR+GN+THHEY+QVGKG
Sbjct: 143 RVRFHYGHPDIFDRLFHLTRGGVSKASKIINLSEDIFAGFNSTLREGNVTHHEYMQVGKG 322
Query: 85 RDVGLNQIALFEGKVAGGNGEQVLSRDV 2
RDVGLNQI+LFE K+A GNGEQ LSRD+
Sbjct: 323 RDVGLNQISLFEAKIAYGNGEQTLSRDI 406
Score = 103 bits (219), Expect(3) = 1e-023
Identities = 49/79 (62%), Positives = 52/79 (65%)
Frame = +3 / -3
Query: 6 SRLRTCSPLPPATFPSNSAIWFKPTSRPFPTWIYSWCVMFPWRKVELNPAYISSLILITR 185
SRLR CSPLP A F SNS I +PTS P PT +YS CV FP R VELNPA +SSL LI
Sbjct: 402 SRLRVCSPLPYAIFASNSEI*LRPTSLPLPTCMYS*CVTFPSRSVELNPANMSSLRLIIL 223
Query: 186 DAXXMPPRVXXNIRSKTSG 242
DA PP V RSK SG
Sbjct: 222 DALLTPPLVRWKSRSKISG 166
Score = 21.2 bits (40), Expect(3) = 1e-023
Identities = 9/21 (42%), Positives = 10/21 (47%)
Frame = +3 / -3
Query: 273 AXXXCPXXTKLXXXXXINXAS 335
A CP TKL +N AS
Sbjct: 132 ASTRCPIVTKLVSWFDMNHAS 70
Score = 19.4 bits (36), Expect(3) = 1e-023
Identities = 6/12 (50%), Positives = 8/12 (66%)
Frame = +1 / -1
Query: 226 DQKHQDXHXXFE 261
DQ++QD H E
Sbjct: 182 DQRYQDVHNEIE 147
>gb|BJ462458.1|BJ462458 BJ462458 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags28g06 5', mRNA sequence
Length = 666
Score = 167 bits (360), Expect = 2e-041
Identities = 68/88 (77%), Positives = 76/88 (86%)
Frame = -2 / +3
Query: 265 KVRXXYXXPDVFDRIFXITRGGIXKASRVINISEDIYAGFNSTLRQGNITHHEYIQVGKG 86
KVR Y PDVFDR++ + RGGI KASRVINISEDI+AGFN TLR GN+THHEYIQVGKG
Sbjct: 72 KVRMHYGHPDVFDRLWFLGRGGISKASRVINISEDIFAGFNCTLRGGNVTHHEYIQVGKG 251
Query: 85 RDVGLNQIALFEGKVAGGNGEQVLSRDV 2
RDVGLNQ+++FE KVA GNGEQ LSRDV
Sbjct: 252 RDVGLNQVSMFEAKVASGNGEQTLSRDV 335
Score = 123 bits (263), Expect = 4e-028
Identities = 51/80 (63%), Positives = 56/80 (70%)
Frame = +3 / -3
Query: 3 TSRLRTCSPLPPATFPSNSAIWFKPTSRPFPTWIYSWCVMFPWRKVELNPAYISSLILIT 182
TSRLR CSPLP AT SN W PTS P PTW+YSWCV P R+V+LNPA ISSL+LIT
Sbjct: 334 TSRLRVCSPLPLATLASNIETWLSPTSLPLPTWMYSWCVTLPPRRVQLNPAKISSLMLIT 155
Query: 183 RDAXXMPPRVXXNIRSKTSG 242
DA +PPR RS TSG
Sbjct: 154 LDALLIPPRPKNQRRSNTSG 95
>gb|BU969103.1|BU969103 HB10J09r BC Hordeum vulgare subsp. vulgare cDNA clone HB10J09
5-PRIME, mRNA sequence
Length = 426
Score = 137 bits (294), Expect = 2e-032
Identities = 56/58 (96%), Positives = 58/58 (100%)
Frame = -2 / +2
Query: 175 NISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
NISEDI+AGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRD+
Sbjct: 11 NISEDIFAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDI 184
Score = 108 bits (231), Expect = 1e-023
Identities = 45/58 (77%), Positives = 47/58 (81%)
Frame = +3 / -1
Query: 6 SRLRTCSPLPPATFPSNSAIWFKPTSRPFPTWIYSWCVMFPWRKVELNPAYISSLILI 179
S LRTCSP PPATFPSNSAI PTS P PTWIYSW VMFP R+VELNPA ISSLI +
Sbjct: 180 SLLRTCSPFPPATFPSNSAI*LSPTSLPLPTWIYSW*VMFP*RRVELNPANISSLIFL 7
>gb|AV836635.1|AV836635 AV836635 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd19k01, mRNA
sequence
Length = 644
Score = 121 bits (258), Expect = 2e-027
Identities = 49/50 (98%), Positives = 50/50 (100%)
Frame = -2 / +2
Query: 151 GFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
GFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRD+
Sbjct: 5 GFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDI 154
>gb|AV934137.1|AV934137 AV934137 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal11c09 5', mRNA sequence
Length = 589
Score = 120 bits (256), Expect = 3e-027
Identities = 46/57 (80%), Positives = 54/57 (94%)
Frame = -2 / +1
Query: 172 ISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
+SEDI+AGFNSTLR+GN+THHEY+QVGKGRDVGLNQI+LFE K+A GNGEQ LSRD+
Sbjct: 7 LSEDIFAGFNSTLREGNVTHHEYMQVGKGRDVGLNQISLFEAKIAYGNGEQTLSRDI 177
>gb|BJ470174.1|BJ470174 BJ470174 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal15l17 5', mRNA sequence
Length = 537
Score = 120 bits (256), Expect = 3e-027
Identities = 46/57 (80%), Positives = 54/57 (94%)
Frame = -2 / +3
Query: 172 ISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
+SEDI+AGFNSTLR+GN+THHEY+QVGKGRDVGLNQI+LFE K+A GNGEQ LSRD+
Sbjct: 18 LSEDIFAGFNSTLREGNVTHHEYMQVGKGRDVGLNQISLFEAKIAYGNGEQTLSRDI 188
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 117,300,183
Number of Sequences: 312970
Number of extensions: 1636424
Number of successful extensions: 126180
Number of sequences better than 1.0e-020: 10
length of database: 58,378,179
effective HSP length: 49
effective length of database: 43,042,649
effective search space used: 2668644238
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)