TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAE8g03.yg.2.1
(360 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CB880803.1|CB880803 HM07O02w HM Hordeum vulgare subsp. v... 145 3e-043
gb|BM442430.2|BM442430 EBan01_SQ003_C15_R anther, yellow st... 139 5e-041
gb|BU994139.1|BU994139 HM05O02r HM Hordeum vulgare subsp. v... 109 2e-032
gb|BM442070.2|BM442070 EBan01_SQ002_B15_R anther, yellow st... 86 7e-030
>gb|CB880803.1|CB880803 HM07O02w HM Hordeum vulgare subsp. vulgare cDNA clone HM07O02
3-PRIME, mRNA sequence
Length = 626
Score = 145 bits (312), Expect(2) = 3e-043
Identities = 60/71 (84%), Positives = 64/71 (90%)
Frame = -3 / -1
Query: 214 RYLFVRWGLYVGNMYLITGEHGDTDHEEMLSGLHKTVIVRGVTEKGSEGLLRSPGSYKKD 35
RYLFVRWGL VGNMYLI GEHGDTD EEMLSGLHKTVIV+GVTEKGSE LLRS GSY K+
Sbjct: 527 RYLFVRWGLAVGNMYLIVGEHGDTDREEMLSGLHKTVIVKGVTEKGSEDLLRSSGSYHKE 348
Query: 34 DVVPSETPLAA 2
DVVP ++PLAA
Sbjct: 347 DVVPVDSPLAA 315
Score = 84.9 bits (179), Expect(2) = 1e-023
Identities = 38/69 (55%), Positives = 48/69 (69%)
Frame = +2 / +3
Query: 11 GGLRRHDVVLLVASWAPQQPFRTFLGDTPDNHSLV*PR*HLLMIGVAMFPSDQIHVPHI* 190
G + R +V+L+V APQ+ R FL D D+ LV* HLL IGVAM P+DQ+HVPH
Sbjct: 324 GAIDRDNVLLVVTP*APQKILRAFLSDAFDDDRLV*S*KHLLAIGVAMLPNDQVHVPHGQ 503
Query: 191 APAHKKIPE 217
APAHK++PE
Sbjct: 504 APAHKEVPE 530
Score = 83.1 bits (175), Expect(2) = 2e-023
Identities = 40/71 (56%), Positives = 45/71 (63%)
Frame = -1 / -2
Query: 222 KNSGIFLCAGAYMWGTCI*SLGNMATPIMRRCYLGYTRL*LSGVSPRKVRKGC*GAQEAT 43
K+SG LCAGA+ WGTC *SLG+MATPI RRC+ YTR S S RK + *GAQ T
Sbjct: 535 KHSGTSLCAGAWPWGTCT*SLGSMATPIARRCFQDYTRRSSSKASLRKALRIF*GAQGVT 356
Query: 42 RRTTSCRLRPP 10
R T R P
Sbjct: 355 TRRTLSRSIAP 323
Score = 47.3 bits (97), Expect(2) = 3e-043
Identities = 19/21 (90%), Positives = 21/21 (100%)
Frame = -3 / -1
Query: 358 CRNSTRLQVVPLLASRSQALR 296
CRNSTR+QVVPL+ASRSQALR
Sbjct: 587 CRNSTRMQVVPLMASRSQALR 525
Score = 43.7 bits (89), Expect(2) = 2e-023
Identities = 17/22 (77%), Positives = 19/22 (86%)
Frame = -1 / -2
Query: 360 TAGTRQGFRLSLC*HQGHRHSG 295
TAGTR+G RLSL HQGH+HSG
Sbjct: 589 TAGTRRGCRLSLSWHQGHKHSG 524
Score = 42.3 bits (86), Expect(2) = 1e-023
Identities = 17/22 (77%), Positives = 19/22 (86%)
Frame = +2 / +3
Query: 293 LPECL*P*C*QRDNLKPCRVPA 358
+PECL*P*C +RDNL P RVPA
Sbjct: 522 VPECL*P*CHERDNLHPRRVPA 587
>gb|BM442430.2|BM442430 EBan01_SQ003_C15_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_C15 5', mRNA sequence
Length = 504
Score = 139 bits (298), Expect(2) = 5e-041
Identities = 57/71 (80%), Positives = 63/71 (88%)
Frame = -3 / +1
Query: 214 RYLFVRWGLYVGNMYLITGEHGDTDHEEMLSGLHKTVIVRGVTEKGSEGLLRSPGSYKKD 35
+YLFVRWGL VGNMYLI GEHGDTD EEMLSGLHKTVIV+GVTEKGSE LLRS SY K+
Sbjct: 250 KYLFVRWGLAVGNMYLIVGEHGDTDREEMLSGLHKTVIVKGVTEKGSEDLLRSSRSYHKE 429
Query: 34 DVVPSETPLAA 2
DVVP ++PL+A
Sbjct: 430 DVVPVDSPLSA 462
Score = 81.7 bits (172), Expect(2) = 3e-022
Identities = 39/73 (53%), Positives = 46/73 (63%)
Frame = -1 / +2
Query: 222 KNSGIFLCAGAYMWGTCI*SLGNMATPIMRRCYLGYTRL*LSGVSPRKVRKGC*GAQEAT 43
K+S LCAGA+ WGTC *SLG+MATPI RRC+ YTR S S RK + *GAQ T
Sbjct: 242 KHSSTSLCAGAWPWGTCT*SLGSMATPIARRCFQDYTRRSSSKASLRKALRIF*GAQGVT 421
Query: 42 RRTTSCRLRPPWL 4
+ T R P+L
Sbjct: 422 TKKTLSRSIAPYL 460
Score = 77.6 bits (163), Expect(2) = 3e-021
Identities = 36/69 (52%), Positives = 45/69 (65%)
Frame = +2 / -1
Query: 11 GGLRRHDVVLLVASWAPQQPFRTFLGDTPDNHSLV*PR*HLLMIGVAMFPSDQIHVPHI* 190
G + R +V +V APQ+ R FL D D+ LV* HLL IGVAM P+DQ+HVPH
Sbjct: 453 GAIDRDNVFFVVTP*APQKILRAFLSDAFDDDRLV*S*KHLLAIGVAMLPNDQVHVPHGQ 274
Query: 191 APAHKKIPE 217
APAHK++ E
Sbjct: 273 APAHKEVLE 247
Score = 46.0 bits (94), Expect(2) = 5e-041
Identities = 18/21 (85%), Positives = 21/21 (100%)
Frame = -3 / +1
Query: 358 CRNSTRLQVVPLLASRSQALR 296
CRNSTR+QVVPL+ASRSQAL+
Sbjct: 190 CRNSTRMQVVPLMASRSQALK 252
Score = 41.4 bits (84), Expect(2) = 3e-021
Identities = 18/22 (81%), Positives = 18/22 (81%)
Frame = +3 / -2
Query: 294 YLSACDLDASRGTT*SLVEFLQ 359
YLSACDLDA RGTT LVEF Q
Sbjct: 254 YLSACDLDAMRGTTCILVEFRQ 189
Score = 40.9 bits (83), Expect(2) = 3e-022
Identities = 16/21 (76%), Positives = 18/21 (85%)
Frame = -1 / +2
Query: 360 TAGTRQGFRLSLC*HQGHRHS 298
TAGTR+G RLSL HQGH+HS
Sbjct: 188 TAGTRRGCRLSLSWHQGHKHS 250
>gb|BU994139.1|BU994139 HM05O02r HM Hordeum vulgare subsp. vulgare cDNA clone HM05O02
5-PRIME, mRNA sequence
Length = 593
Score = 109 bits (233), Expect(2) = 2e-032
Identities = 44/48 (91%), Positives = 45/48 (93%)
Frame = -3 / +2
Query: 214 RYLFVRWGLYVGNMYLITGEHGDTDHEEMLSGLHKTVIVRGVTEKGSE 71
RYLFVRWGL VGNMYLI GEHGDTD EEMLSGLHKTVIV+GVTEKGSE
Sbjct: 449 RYLFVRWGLAVGNMYLIVGEHGDTDREEMLSGLHKTVIVKGVTEKGSE 592
Score = 47.3 bits (97), Expect(2) = 2e-032
Identities = 19/21 (90%), Positives = 21/21 (100%)
Frame = -3 / +2
Query: 358 CRNSTRLQVVPLLASRSQALR 296
CRNSTR+QVVPL+ASRSQALR
Sbjct: 389 CRNSTRMQVVPLMASRSQALR 451
>gb|BM442070.2|BM442070 EBan01_SQ002_B15_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ002_B15 5', mRNA sequence
Length = 260
Score = 85.8 bits (181), Expect(2) = 7e-030
Identities = 34/43 (79%), Positives = 36/43 (83%)
Frame = -3 / +1
Query: 229 LQKKFRYLFVRWGLYVGNMYLITGEHGDTDHEEMLSGLHKTVI 101
+ + RYLFVRWGL VGNMYLI GEHGDTD EEMLS LHKTVI
Sbjct: 13 MSQALRYLFVRWGLAVGNMYLIVGEHGDTDREEMLSRLHKTVI 141
Score = 61.1 bits (127), Expect(2) = 7e-030
Identities = 25/33 (75%), Positives = 29/33 (87%)
Frame = -3 / +2
Query: 100 VRGVTEKGSEGLLRSPGSYKKDDVVPSETPLAA 2
V+GVTEKGSE LLRS GSY K+DVVP ++PLAA
Sbjct: 143 VKGVTEKGSEDLLRSSGSYHKEDVVPGDSPLAA 241
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 190,786,827
Number of Sequences: 312970
Number of extensions: 3116812
Number of successful extensions: 208537
Number of sequences better than 1.0e-020: 4
length of database: 58,378,179
effective HSP length: 49
effective length of database: 43,042,649
effective search space used: 3012985430
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)