TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 9232502.2.1
(560 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF618071.3|BF618071 HVSMEc0003K24f Hordeum vulgare seedl... 168 2e-041
gb|BI957520.1|BI957520 HVSMEn0009P14f Hordeum vulgare rachi... 165 2e-040
gb|BI959765.1|BI959765 HVSMEn0021F16f Hordeum vulgare rachi... 163 8e-040
gb|BG310062.1|BG310062 HVSMEc0016D20f Hordeum vulgare seedl... 116 5e-034
gb|CA024360.1|CA024360 HZ49B12r HZ Hordeum vulgare subsp. v... 126 2e-033
gb|BI960243.1|BI960243 HVSMEn0023O08f Hordeum vulgare rachi... 126 9e-029
gb|CA019501.1|CA019501 HV12C24r HV Hordeum vulgare subsp. v... 111 3e-024
gb|BQ763227.1|BQ763227 EBro02_SQ007_J17_R root, 3 week, hyd... 100 5e-021
gb|CA007155.1|CA007155 HU06P08r HU Hordeum vulgare subsp. v... 100 5e-021
>gb|BF618071.3|BF618071 HVSMEc0003K24f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0003K24f, mRNA sequence
Length = 771
Score = 168 bits (361), Expect = 2e-041
Identities = 70/94 (74%), Positives = 80/94 (85%)
Frame = -2 / +2
Query: 388 KDEQFSEFRQLLHDTRVDTQINAQLTDSYNGLTIMAPTNAAFDKMKAGVLNGLSPQEQIQ 209
K ++S+F LL +TRV+TQINAQLTDSYNGLTI APT+AAFD +KAG N L+ QEQIQ
Sbjct: 248 KSGKYSKFLALLKETRVETQINAQLTDSYNGLTIFAPTDAAFDGLKAGTFNTLTSQEQIQ 427
Query: 208 MVLYCVLPRFYSLSMLGTLSGKVNTQGSGHDGPY 107
MVLYCVLP FYSLSMLGTL+GKV+TQ SGH GPY
Sbjct: 428 MVLYCVLPSFYSLSMLGTLNGKVSTQASGHSGPY 529
Score = 136 bits (292), Expect = 8e-032
Identities = 62/80 (77%), Positives = 63/80 (78%)
Frame = +3 / -1
Query: 108 *GPSWPDPCVLTLPLRVPSMDRL*NRGSTQ*STIWICSCGDSPFSTPAFILSKAALVGAM 287
*GP WPD CVLTLPLRVPSM+ * GSTQ*STIWICSC S PA SKAA VGA
Sbjct: 528 *GPEWPDACVLTLPLRVPSMESE*KLGSTQ*STIWICSCDVSVLKVPALSPSKAASVGAK 349
Query: 288 MVSPL*LSVSCALICVSTRV 347
MVSPL*LSVSCALICVSTRV
Sbjct: 348 MVSPL*LSVSCALICVSTRV 289
Score = 127 bits (272), Expect = 5e-029
Identities = 56/76 (73%), Positives = 62/76 (81%)
Frame = +2 / -3
Query: 143 LAAEGAEHGQAVEPGQHAVEHHLDLLLRRQPVQHARLHLVEGGVGGRHDGQPVVAVRQLR 322
LA EGAEHG+ VE QHAVEHHLDLLLRRQ V+ A L VEGGVGG DGQPVVAVRQLR
Sbjct: 493 LAVEGAEHGERVEARQHAVEHHLDLLLRRQRVEGAGLEPVEGGVGGGEDGQPVVAVRQLR 314
Query: 323 VDLRVHARVVQQLPEL 370
VDLR+HA +++Q EL
Sbjct: 313 VDLRLHAGLLEQRQEL 266
Score = 105 bits (225), Expect(2) = 9e-023
Identities = 47/74 (63%), Positives = 52/74 (70%)
Frame = -1 / +1
Query: 329 DQRAADGQLQRADHHGAHQRRLRQDXXXXXXXXXXXXADPDGALLRAAPVLQPVHARHPQ 150
DQRAADGQLQRADH H+RRLR+ ADPDGALLRAA +L +HARHPQ
Sbjct: 307 DQRAADGQLQRADHLRPHRRRLRRAQGRHLQHADVAGADPDGALLRAAELLLALHARHPQ 486
Query: 149 RQGQHAGVRPRRAL 108
RQG+HA VRP R L
Sbjct: 487 RQGEHASVRPLRPL 528
Score = 21.7 bits (41), Expect(2) = 9e-023
Identities = 7/14 (50%), Positives = 12/14 (85%)
Frame = -1 / +2
Query: 104 VRHQAVGEQRQRVH 63
++ QA+ +QRQR+H
Sbjct: 533 LQEQALRQQRQRLH 574
>gb|BI957520.1|BI957520 HVSMEn0009P14f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0009P14f, mRNA sequence
Length = 671
Score = 165 bits (354), Expect = 2e-040
Identities = 70/99 (70%), Positives = 80/99 (80%)
Frame = -2 / +2
Query: 388 KDEQFSEFRQLLHDTRVDTQINAQLTDSYNGLTIMAPTNAAFDKMKAGVLNGLSPQEQIQ 209
K ++S+F LL +TRV+TQINAQLTDSYNGLTI APT+AAFD +KAG N L+ QEQIQ
Sbjct: 275 KSGKYSKFLALLKETRVETQINAQLTDSYNGLTIFAPTDAAFDGLKAGTFNTLTSQEQIQ 454
Query: 208 MVLYCVLPRFYSLSMLGTLSGKVNTQGSGHDGPYRYDIK 92
MVLYCVLPR YSL MLGTL+GKV+TQ S H PY Y IK
Sbjct: 455 MVLYCVLPRXYSLYMLGTLNGKVSTQASRHSSPYTYKIK 571
Score = 124 bits (266), Expect = 3e-028
Identities = 59/81 (72%), Positives = 60/81 (74%)
Frame = +3 / -3
Query: 105 L*GPSWPDPCVLTLPLRVPSMDRL*NRGSTQ*STIWICSCGDSPFSTPAFILSKAALVGA 284
+*G W D CVLT PLRVPSM RGSTQ*STIWICSC S PA SKAA VGA
Sbjct: 558 V*GLEWRDACVLTFPLRVPSM*SEXKRGSTQ*STIWICSCDVSVLKVPALSPSKAASVGA 379
Query: 285 MMVSPL*LSVSCALICVSTRV 347
MVSPL*LSVSCALICVSTRV
Sbjct: 378 KMVSPL*LSVSCALICVSTRV 316
Score = 118 bits (252), Expect = 3e-026
Identities = 53/76 (69%), Positives = 59/76 (77%)
Frame = +2 / -2
Query: 143 LAAEGAEHGQAVEPGQHAVEHHLDLLLRRQPVQHARLHLVEGGVGGRHDGQPVVAVRQLR 322
L EGAEH + V QHAVEHHLDLLLRRQ V+ A L VEGGVGG DGQPVVAVRQLR
Sbjct: 520 LPVEGAEHVERVXARQHAVEHHLDLLLRRQRVEGAGLEPVEGGVGGGEDGQPVVAVRQLR 341
Query: 323 VDLRVHARVVQQLPEL 370
VDLR+HA +++Q EL
Sbjct: 340 VDLRLHAGLLEQRQEL 293
Score = 104 bits (221), Expect = 5e-022
Identities = 46/84 (54%), Positives = 57/84 (67%)
Frame = -1 / +1
Query: 329 DQRAADGQLQRADHHGAHQRRLRQDXXXXXXXXXXXXADPDGALLRAAPVLQPVHARHPQ 150
DQRAADGQLQRADH H+RRLR+ ADPDGALLRAA + +HARHPQ
Sbjct: 334 DQRAADGQLQRADHLRPHRRRLRRAQGRHLQHADVAGADPDGALLRAAALXLALHARHPQ 513
Query: 149 RQGQHAGVRPRRALQVRHQAVGEQ 78
R+G+HAGV P + L ++ +A +Q
Sbjct: 514 REGEHAGVPPLQPLHLQDKAPRQQ 585
>gb|BI959765.1|BI959765 HVSMEn0021F16f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0021F16f, mRNA sequence
Length = 662
Score = 163 bits (350), Expect = 8e-040
Identities = 68/94 (72%), Positives = 79/94 (84%)
Frame = -2 / +1
Query: 388 KDEQFSEFRQLLHDTRVDTQINAQLTDSYNGLTIMAPTNAAFDKMKAGVLNGLSPQEQIQ 209
K ++S+F LL +TRV+TQINAQLTDSYNGLTI APT+AAFD +KAG N L+ QEQIQ
Sbjct: 286 KSGKYSKFLALLKETRVETQINAQLTDSYNGLTIFAPTDAAFDGLKAGTFNTLTSQEQIQ 465
Query: 208 MVLYCVLPRFYSLSMLGTLSGKVNTQGSGHDGPY 107
MVLYCVLPRFYSLSMLGTL+G+V+T S H GPY
Sbjct: 466 MVLYCVLPRFYSLSMLGTLNGQVSTHASRHSGPY 567
Score = 133 bits (284), Expect(2) = 4e-032
Identities = 61/80 (76%), Positives = 62/80 (77%)
Frame = +3 / -1
Query: 108 *GPSWPDPCVLTLPLRVPSMDRL*NRGSTQ*STIWICSCGDSPFSTPAFILSKAALVGAM 287
*GP W D CVLT PLRVPSM+ * RGSTQ*STIWICSC S PA SKAA VGA
Sbjct: 566 *GPEWRDACVLTWPLRVPSMESE*KRGSTQ*STIWICSCDVSVLKVPALSPSKAASVGAK 387
Query: 288 MVSPL*LSVSCALICVSTRV 347
MVSPL*LSVSCALICVSTRV
Sbjct: 386 MVSPL*LSVSCALICVSTRV 327
Score = 125 bits (268), Expect = 2e-028
Identities = 55/76 (72%), Positives = 62/76 (81%)
Frame = +2 / -3
Query: 143 LAAEGAEHGQAVEPGQHAVEHHLDLLLRRQPVQHARLHLVEGGVGGRHDGQPVVAVRQLR 322
LA +GAEHG+ VE QHAVEHHLDLLLRRQ V+ A L VEGGVGG DGQPVVAVRQLR
Sbjct: 531 LAVKGAEHGERVEARQHAVEHHLDLLLRRQRVEGAGLEPVEGGVGGGEDGQPVVAVRQLR 352
Query: 323 VDLRVHARVVQQLPEL 370
VDLR+HA +++Q EL
Sbjct: 351 VDLRLHAGLLEQRQEL 304
Score = 24.4 bits (47), Expect(2) = 4e-032
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +3 / -2
Query: 48 IQFTPVDTLTLFPDRLM 98
+ TPV TLTL P+ L+
Sbjct: 625 VPLTPVKTLTLLPEGLI 575
>gb|BG310062.1|BG310062 HVSMEc0016D20f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0016D20f, mRNA sequence
Length = 823
Score = 116 bits (248), Expect(2) = 5e-034
Identities = 50/74 (67%), Positives = 59/74 (79%)
Frame = -2 / +2
Query: 388 KDEQFSEFRQLLHDTRVDTQINAQLTDSYNGLTIMAPTNAAFDKMKAGVLNGLSPQEQIQ 209
K ++S+F LL +TRV+TQINAQLTDSY GLTI A T+A FD +KAG LN L+ QEQI
Sbjct: 260 KSGKYSKFLALLKETRVETQINAQLTDSYYGLTIFAHTDAGFDGLKAGTLNTLTSQEQIH 439
Query: 208 MVLYCVLPRFYSLS 167
MVLYCVLPRFY +S
Sbjct: 440 MVLYCVLPRFYLIS 481
Score = 102 bits (217), Expect(2) = 1e-025
Identities = 47/72 (65%), Positives = 52/72 (72%)
Frame = +2 / -1
Query: 155 GAEHGQAVEPGQHAVEHHLDLLLRRQPVQHARLHLVEGGVGGRHDGQPVVAVRQLRVDLR 334
G G VE QHAVEHH+DLLLRRQ VQ L VE GVG DGQPVVAVRQLRVDLR
Sbjct: 493 GYRAGDEVEARQHAVEHHVDLLLRRQRVQGTGLEPVEAGVGVGEDGQPVVAVRQLRVDLR 314
Query: 335 VHARVVQQLPEL 370
+HA +++Q EL
Sbjct: 313 LHAGLLEQRQEL 278
Score = 47.3 bits (97), Expect(2) = 5e-034
Identities = 17/34 (50%), Positives = 26/34 (76%)
Frame = -1 / +3
Query: 164 ARHPQRQGQHAGVRPRRALQVRHQAVGEQRQRVH 63
AR+PQRQG+H GVR R+L ++ +A+ + RQ +H
Sbjct: 483 ARYPQRQGEHPGVRALRSLHLQDRALAQHRQVIH 584
Score = 33.1 bits (66), Expect(2) = 1e-025
Identities = 17/36 (47%), Positives = 21/36 (58%)
Frame = +3 / -3
Query: 54 FTPVDTLTLFPDRLMSYL*GPSWPDPCVLTLPLRVP 161
FT VD LT+ + +* P + VLTLPLRVP
Sbjct: 593 FTTVDNLTMLRQGSIL*V*RPEGTNAWVLTLPLRVP 486
>gb|CA024360.1|CA024360 HZ49B12r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ49B12
5-PRIME, mRNA sequence
Length = 571
Score = 126 bits (270), Expect(2) = 2e-033
Identities = 52/67 (77%), Positives = 57/67 (85%)
Frame = -2 / +3
Query: 292 TIMAPTNAAFDKMKAGVLNGLSPQEQIQMVLYCVLPRFYSLSMLGTLSGKVNTQGSGHDG 113
TI APT+AAFD +KAG N L+ QEQIQMVLYCVLPRFYSLSMLGTL+GKV+TQ SGH G
Sbjct: 12 TIFAPTDAAFDGLKAGTFNTLTSQEQIQMVLYCVLPRFYSLSMLGTLNGKVSTQASGHSG 191
Query: 112 PYRYDIK 92
PY Y IK
Sbjct: 192 PYTYKIK 212
Score = 117 bits (251), Expect(2) = 1e-030
Identities = 53/82 (64%), Positives = 59/82 (71%)
Frame = +3 / -1
Query: 48 IQFTPVDTLTLFPDRLMSYL*GPSWPDPCVLTLPLRVPSMDRL*NRGSTQ*STIWICSCG 227
+ TPV+TLTL + L+ +*GP WPD CVLTLPLRVPSM+ * RGSTQ*STIWICSC
Sbjct: 256 LPLTPVETLTLLAEGLIL*V*GPEWPDACVLTLPLRVPSMESE*KRGSTQ*STIWICSCD 77
Query: 228 DSPFSTPAFILSKAALVGAMMV 293
S PA SKAA VGA MV
Sbjct: 76 VSVLKVPALSPSKAASVGAKMV 11
Score = 100 bits (212), Expect(2) = 1e-023
Identities = 43/77 (55%), Positives = 53/77 (68%)
Frame = -1 / +2
Query: 293 DHHGAHQRRLRQDXXXXXXXXXXXXADPDGALLRAAPVLQPVHARHPQRQGQHAGVRPRR 114
DH H+RRLR+ ADPDGALLRAA +L +HARHPQRQG+HAGVRP R
Sbjct: 11 DHLRPHRRRLRRAQGRHLQHADVAGADPDGALLRAAALLLALHARHPQRQGEHAGVRPLR 190
Query: 113 ALQVRHQAVGEQRQRVH 63
L ++ QA+ +QRQR+H
Sbjct: 191 PLHLQDQALRQQRQRLH 241
Score = 35.4 bits (71), Expect(2) = 2e-033
Identities = 14/16 (87%), Positives = 15/16 (93%)
Frame = -2 / +3
Query: 49 MLLGSPVSKDFPLAIY 2
MLLGS VSKDFPLA+Y
Sbjct: 261 MLLGSVVSKDFPLAVY 308
Score = 35.0 bits (70), Expect(2) = 1e-030
Identities = 14/18 (77%), Positives = 16/18 (88%)
Frame = +2 / -3
Query: 2 VDGEREVLADGAAQQHPV 55
VDGEREVLAD AA++H V
Sbjct: 308 VDGEREVLADDAAEEHVV 255
Score = 29.0 bits (57), Expect(2) = 1e-023
Identities = 14/26 (53%), Positives = 15/26 (57%)
Frame = -1 / +2
Query: 80 QRQRVHGGELDAAGQPRQQGLPARHL 3
QR G+ A Q RQQGLPAR L
Sbjct: 230 QRLHRRQGQQHAPRQRRQQGLPARRL 307
>gb|BI960243.1|BI960243 HVSMEn0023O08f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0023O08f, mRNA sequence
Length = 492
Score = 126 bits (270), Expect = 9e-029
Identities = 53/72 (73%), Positives = 61/72 (84%)
Frame = -2 / +3
Query: 388 KDEQFSEFRQLLHDTRVDTQINAQLTDSYNGLTIMAPTNAAFDKMKAGVLNGLSPQEQIQ 209
K ++S+F LL +TRV+TQINAQLTDSYNGLTI APT+AAFD +KAG N L+ QEQIQ
Sbjct: 276 KSGKYSKFLALLKETRVETQINAQLTDSYNGLTIFAPTDAAFDGLKAGTFNTLTSQEQIQ 455
Query: 208 MVLYCVLPRFYS 173
MVLYCVLPRFYS
Sbjct: 456 MVLYCVLPRFYS 491
Score = 107 bits (229), Expect = 4e-023
Identities = 48/65 (73%), Positives = 53/65 (81%)
Frame = +2 / -2
Query: 176 VEPGQHAVEHHLDLLLRRQPVQHARLHLVEGGVGGRHDGQPVVAVRQLRVDLRVHARVVQ 355
VE QHAVEHHLDLLLRRQ V+ A L VEGGVGG DGQPVVAVRQLRVDLR+HA +++
Sbjct: 488 VEARQHAVEHHLDLLLRRQRVEGAGLEPVEGGVGGGEDGQPVVAVRQLRVDLRLHAGLLE 309
Query: 356 QLPEL 370
Q EL
Sbjct: 308 QRQEL 294
>gb|CA019501.1|CA019501 HV12C24r HV Hordeum vulgare subsp. vulgare cDNA clone HV12C24
5-PRIME, mRNA sequence
Length = 494
Score = 111 bits (237), Expect = 3e-024
Identities = 47/66 (71%), Positives = 55/66 (83%)
Frame = -2 / +1
Query: 388 KDEQFSEFRQLLHDTRVDTQINAQLTDSYNGLTIMAPTNAAFDKMKAGVLNGLSPQEQIQ 209
K ++S+F LL +TRV+TQINAQLTDSYNGLTI APT+AAFD +KAG N L+ QEQIQ
Sbjct: 295 KSGKYSKFLALLKETRVETQINAQLTDSYNGLTIFAPTDAAFDGLKAGTFNTLTSQEQIQ 474
Query: 208 MVLYCV 191
MVLYCV
Sbjct: 475 MVLYCV 492
Score = 102 bits (218), Expect = 1e-021
Identities = 45/60 (75%), Positives = 50/60 (83%)
Frame = +2 / -3
Query: 191 HAVEHHLDLLLRRQPVQHARLHLVEGGVGGRHDGQPVVAVRQLRVDLRVHARVVQQLPEL 370
HAVEHHLDLLLRRQ V+ A L VEGGVGG DGQPVVAVRQLRVDLR+HA +++Q EL
Sbjct: 492 HAVEHHLDLLLRRQRVEGAGLEPVEGGVGGGEDGQPVVAVRQLRVDLRLHAGLLEQRQEL 313
>gb|BQ763227.1|BQ763227 EBro02_SQ007_J17_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ007_J17 5', mRNA sequence
Length = 581
Score = 100 bits (214), Expect = 5e-021
Identities = 42/93 (45%), Positives = 60/93 (64%)
Frame = -2 / +2
Query: 388 KDEQFSEFRQLLHDTRVDTQINAQLTDSYNGLTIMAPTNAAFDKMKAGVLNGLSPQEQIQ 209
K Q+++F +L+ T+ DTQ+NAQ +S G T+ APT+ AF+ +K G LN LS Q+Q+
Sbjct: 218 KAGQYTKFIRLMASTQQDTQLNAQANESDTGFTVFAPTDNAFNSLKPGTLNSLSQQDQVT 397
Query: 208 MVLYCVLPRFYSLSMLGTLSGKVNTQGSGHDGP 110
+V ++P FYS+ T S V TQ SG DGP
Sbjct: 398 LVQAHIVPTFYSMESFETASNPVRTQASGTDGP 496
>gb|CA007155.1|CA007155 HU06P08r HU Hordeum vulgare subsp. vulgare cDNA clone HU06P08
5-PRIME, mRNA sequence
Length = 396
Score = 100 bits (214), Expect(2) = 5e-021
Identities = 42/93 (45%), Positives = 60/93 (64%)
Frame = -2 / +2
Query: 388 KDEQFSEFRQLLHDTRVDTQINAQLTDSYNGLTIMAPTNAAFDKMKAGVLNGLSPQEQIQ 209
K Q+++F +L+ T+ DTQ+NAQ +S G T+ APT+ AF+ +K G LN LS Q+Q+
Sbjct: 17 KAGQYTKFIRLMASTQQDTQLNAQANESDTGFTVFAPTDNAFNSLKPGTLNSLSQQDQVT 196
Query: 208 MVLYCVLPRFYSLSMLGTLSGKVNTQGSGHDGP 110
+V ++P FYS+ T S V TQ SG DGP
Sbjct: 197 LVQAHIVPTFYSMESFETASNPVRTQASGTDGP 295
Score = 18.9 bits (35), Expect(2) = 5e-021
Identities = 6/13 (46%), Positives = 10/13 (76%)
Frame = -1 / +1
Query: 101 RHQAVGEQRQRVH 63
RH+ ++RQR+H
Sbjct: 307 RHRHQQQRRQRLH 345
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 169,728,580
Number of Sequences: 312970
Number of extensions: 2560966
Number of successful extensions: 90865
Number of sequences better than 1.0e-020: 10
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 5811236344
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)