TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 8635788.2.1
(753 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF618284.2|BF618284 HVSMEc0005E05f Hordeum vulgare seedl... 150 4e-061
gb|BG344696.1|BG344696 HVSMEg0015C23f Hordeum vulgare pre-a... 118 4e-037
gb|BI959363.1|BI959363 HVSMEn0019G03f Hordeum vulgare rachi... 122 3e-035
gb|BG300636.1|BG300636 HVSMEb0017M06f Hordeum vulgare seedl... 118 4e-034
gb|BI959905.1|BI959905 HVSMEn0022F07f Hordeum vulgare rachi... 110 1e-031
gb|BE455952.3|BE455952 HVSMEg0017N20f Hordeum vulgare pre-a... 106 1e-022
gb|BM370974.2|BM370974 EBro04_SQ003_B17_R root, 3 week, sal... 106 1e-022
gb|BU978837.1|BU978837 HA14G17r HA Hordeum vulgare subsp. v... 106 1e-022
gb|CA025009.1|CA025009 HZ50P08r HZ Hordeum vulgare subsp. v... 106 1e-022
gb|BF628401.2|BF628401 HVSMEb0003O23f Hordeum vulgare seedl... 98 2e-022
gb|BF254052.2|BF254052 HVSMEf0002N24f Hordeum vulgare seedl... 103 1e-021
gb|BU977817.1|BU977817 HA12B15r HA Hordeum vulgare subsp. v... 102 2e-021
gb|BQ763227.1|BQ763227 EBro02_SQ007_J17_R root, 3 week, hyd... 79 3e-021
>gb|BF618284.2|BF618284 HVSMEc0005E05f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0005E05f, mRNA sequence
Length = 669
Score = 150 bits (322), Expect(3) = 4e-061
Identities = 62/75 (82%), Positives = 70/75 (93%)
Frame = +1 / +1
Query: 283 SNGGLTVFAPPDSAFSALPAGTLNSLSDQQKTSLVQFHVVSAALPAAQLETVSNPLRTQA 462
S+GGLTVFAPPD+AF+AL +G+LNSLSDQQKTSLVQFHVVS LP AQ +TVSNPLRTQA
Sbjct: 226 SSGGLTVFAPPDNAFTALKSGSLNSLSDQQKTSLVQFHVVSQLLPMAQFDTVSNPLRTQA 405
Query: 463 GDTGRGKYPLNLTAD 507
GDTGRGKYPLN+T+D
Sbjct: 406 GDTGRGKYPLNVTSD 450
Score = 145 bits (312), Expect(3) = 2e-041
Identities = 61/75 (81%), Positives = 64/75 (85%)
Frame = -2 / -2
Query: 506 SAVRLSGYLPLPVSPACVRSGLLTVSSCAAGSAADTTWNCTSDVFCWSERELSVPAGSAE 327
S V LSGYLPLPVSPACVRSGLLTVS+CA GS+ +TTWNCTSDVFCWSEREL P SA
Sbjct: 449 SDVTLSGYLPLPVSPACVRSGLLTVSNCAMGSSWETTWNCTSDVFCWSERELREPDLSAV 270
Query: 326 KALSGGANTVSPPLD 282
KALSGGA TV PPLD
Sbjct: 269 KALSGGAKTVRPPLD 225
Score = 72.1 bits (151), Expect(3) = 4e-061
Identities = 31/41 (75%), Positives = 35/41 (85%)
Frame = +1 / +1
Query: 172 KTITAILSKAGQFTKFLQLLQSTREAEQITNQLKGKPSNGG 294
KTI A+L+KAGQFTKFLQLLQST+E EQI QLKGK S+ G
Sbjct: 112 KTIKAVLTKAGQFTKFLQLLQSTQEEEQIDTQLKGKASSSG 234
Score = 70.3 bits (147), Expect(2) = 7e-024
Identities = 28/47 (59%), Positives = 34/47 (72%)
Frame = -2 / -2
Query: 314 GGANTVSPPLDGLPLSWLVICSASRVDCSSWRNLVNCPALLRIAVMV 174
G P + LPLSW+ ICS+S VDCSSWRNLVNCPAL+ A++V
Sbjct: 254 GAKTVRPPLDEALPLSWVSICSSSCVDCSSWRNLVNCPALVSTALIV 114
Score = 60.2 bits (125), Expect(2) = 7e-024
Identities = 31/48 (64%), Positives = 32/48 (66%)
Frame = -1 / -1
Query: 510 AVRGEVERVLAPAGVASLRPQRVADXXXXXXXXXXGHDVELHQRRLLL 367
AV +VERVLAPAGVA LRPQRVA G VELHQRRLLL
Sbjct: 453 AVGRDVERVLAPAGVAGLRPQRVAHRVELRHGEQLGDHVELHQRRLLL 310
Score = 51.5 bits (106), Expect(3) = 4e-061
Identities = 20/22 (90%), Positives = 20/22 (90%)
Frame = +1 / +1
Query: 565 LYAGDRLVVYQVSKVLLPWALY 630
LY GDRLVVYQV KVLLPWALY
Sbjct: 508 LYTGDRLVVYQVDKVLLPWALY 573
Score = 38.2 bits (77), Expect(3) = 2e-041
Identities = 15/23 (65%), Positives = 18/23 (78%)
Frame = -1 / -1
Query: 630 VERPRQQHLADLVDDEPVPGVKR 562
VERPR+QHL LV +PV GV+R
Sbjct: 573 VERPRKQHLVHLVHHQPVAGVER 505
Score = 23.5 bits (45), Expect(3) = 2e-041
Identities = 9/21 (42%), Positives = 12/21 (57%)
Frame = -1 / -1
Query: 561 RGVERGVDDPRGDVDAGAVRG 499
R ++ G+DD GDV A G
Sbjct: 507 RAIDGGIDDAGGDVHPPAAVG 445
>gb|BG344696.1|BG344696 HVSMEg0015C23f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0015C23f, mRNA sequence
Length = 888
Score = 118 bits (252), Expect(3) = 4e-037
Identities = 46/72 (63%), Positives = 61/72 (84%)
Frame = +1 / +1
Query: 292 GLTVFAPPDSAFSALPAGTLNSLSDQQKTSLVQFHVVSAALPAAQLETVSNPLRTQAGDT 471
G+TVFAP D+AF++L GTLNSLSD QK +L+Q+HV+S A+P +Q +TVSNPLRTQAG +
Sbjct: 298 GMTVFAPTDNAFTSLAYGTLNSLSDSQKNALIQYHVLSTAIPMSQFDTVSNPLRTQAGSS 477
Query: 472 GRGKYPLNLTAD 507
G+YPLN+TA+
Sbjct: 478 SPGQYPLNVTAE 513
Score = 91.3 bits (193), Expect(2) = 4e-027
Identities = 44/72 (61%), Positives = 48/72 (66%)
Frame = -2 / -2
Query: 506 SAVRLSGYLPLPVSPACVRSGLLTVSSCAAGSAADTTWNCTSDVFCWSERELSVPAGSAE 327
SAV LSGY P + PACVRSGLLTVS+C G A + TW S F S+ ELSVP S
Sbjct: 512 SAVTLSGYWPGELLPACVRSGLLTVSNCDMGMAVERTWYWISAFFWLSDSELSVP*ASEV 333
Query: 326 KALSGGANTVSP 291
KALS GANTV P
Sbjct: 332 KALSVGANTVMP 297
Score = 50.1 bits (103), Expect(2) = 4e-027
Identities = 22/38 (57%), Positives = 26/38 (68%)
Frame = -2 / -2
Query: 290 PLDGLPLSWLVICSASRVDCSSWRNLVNCPALLRIAVM 177
PL L LSWL IC+A+ VD SS N++NCPAL VM
Sbjct: 299 PLPKLLLSWLSICAATPVDLSSRMNVLNCPALASTPVM 186
Score = 46.0 bits (94), Expect(3) = 4e-037
Identities = 20/38 (52%), Positives = 26/38 (68%)
Frame = +1 / +1
Query: 178 ITAILSKAGQFTKFLQLLQSTREAEQITNQLKGKPSNG 291
IT +L+KAGQF F++LL+ST A QI NQL +G
Sbjct: 187 ITGVLAKAGQFNTFIRLLKSTGVAAQIDNQLNNSFGSG 300
Score = 28.6 bits (56), Expect(3) = 4e-037
Identities = 12/21 (57%), Positives = 16/21 (76%)
Frame = +1 / +3
Query: 565 LYAGDRLVVYQVSKVLLPWAL 627
LY G+ LVV QV+KVL+ A+
Sbjct: 567 LYTGEPLVVVQVNKVLVAMAI 629
>gb|BI959363.1|BI959363 HVSMEn0019G03f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0019G03f, mRNA sequence
Length = 688
Score = 122 bits (261), Expect(2) = 3e-035
Identities = 49/90 (54%), Positives = 67/90 (74%)
Frame = +1 / +1
Query: 292 GLTVFAPPDSAFSALPAGTLNSLSDQQKTSLVQFHVVSAALPAAQLETVSNPLRTQAGDT 471
G+TVFAP D+AF++L +GTLNSLSD QK +L+Q+HV+S A+P ++ +TVSNPLRTQAG +
Sbjct: 286 GMTVFAPTDNAFTSLASGTLNSLSDSQKNALIQYHVLSTAIPMSEFDTVSNPLRTQAGSS 465
Query: 472 GRGKYPLNLTADXXXXXXXXXXXXATLDAT 561
G+YPLN+TA+ AT+D T
Sbjct: 466 SPGQYPLNVTAEGQQVNITTGVVNATVDNT 555
Score = 88.1 bits (186), Expect(2) = 3e-026
Identities = 43/72 (59%), Positives = 47/72 (65%)
Frame = -2 / -3
Query: 506 SAVRLSGYLPLPVSPACVRSGLLTVSSCAAGSAADTTWNCTSDVFCWSERELSVPAGSAE 327
SAV LSGY P + PACVRSGLLTVS+ G A + TW S F S+ ELSVP S
Sbjct: 500 SAVTLSGYWPGELLPACVRSGLLTVSNSDMGMAVERTWYWISAFFWLSDSELSVPEASEV 321
Query: 326 KALSGGANTVSP 291
KALS GANTV P
Sbjct: 320 KALSVGANTVMP 285
Score = 50.1 bits (103), Expect(2) = 3e-026
Identities = 22/38 (57%), Positives = 26/38 (68%)
Frame = -2 / -3
Query: 290 PLDGLPLSWLVICSASRVDCSSWRNLVNCPALLRIAVM 177
PL L LSWL IC+A+ VD SS N++NCPAL VM
Sbjct: 287 PLPKLLLSWLSICAATPVDLSSRMNVLNCPALASTPVM 174
Score = 46.0 bits (94), Expect(2) = 3e-035
Identities = 20/38 (52%), Positives = 26/38 (68%)
Frame = +1 / +1
Query: 178 ITAILSKAGQFTKFLQLLQSTREAEQITNQLKGKPSNG 291
IT +L+KAGQF F++LL+ST A QI NQL +G
Sbjct: 175 ITGVLAKAGQFNTFIRLLKSTGVAAQIDNQLNNSFGSG 288
>gb|BG300636.1|BG300636 HVSMEb0017M06f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0017M06f, mRNA sequence
Length = 589
Score = 118 bits (253), Expect(2) = 4e-034
Identities = 48/90 (53%), Positives = 65/90 (72%)
Frame = +1 / +1
Query: 292 GLTVFAPPDSAFSALPAGTLNSLSDQQKTSLVQFHVVSAALPAAQLETVSNPLRTQAGDT 471
G+TVFAP D+AF++L GTLNSLSD QK +L+Q+HV+S A+P +Q +TV +PLRTQAG +
Sbjct: 277 GMTVFAPTDNAFTSLACGTLNSLSDSQKNALIQYHVLSTAIPMSQFDTVRHPLRTQAGSS 456
Query: 472 GRGKYPLNLTADXXXXXXXXXXXXATLDAT 561
G+YPLN+TA+ AT+D T
Sbjct: 457 SPGQYPLNVTAEGPAVNITTGVVYATVDNT 546
Score = 90.4 bits (191), Expect(2) = 7e-027
Identities = 43/72 (59%), Positives = 47/72 (65%)
Frame = -2 / -3
Query: 506 SAVRLSGYLPLPVSPACVRSGLLTVSSCAAGSAADTTWNCTSDVFCWSERELSVPAGSAE 327
SAV LSGY P + PACVRSG LTVS+C G A + TW S F S+ ELSVP S
Sbjct: 491 SAVTLSGYWPGELLPACVRSGCLTVSNCDMGMAVERTWYWISAFFWLSDSELSVPQASEV 312
Query: 326 KALSGGANTVSP 291
KALS GANTV P
Sbjct: 311 KALSVGANTVMP 276
Score = 50.1 bits (103), Expect(2) = 7e-027
Identities = 22/38 (57%), Positives = 26/38 (68%)
Frame = -2 / -3
Query: 290 PLDGLPLSWLVICSASRVDCSSWRNLVNCPALLRIAVM 177
PL L LSWL IC+A+ VD SS N++NCPAL VM
Sbjct: 278 PLPKLLLSWLSICAATPVDLSSRMNVLNCPALASTPVM 165
Score = 46.0 bits (94), Expect(2) = 4e-034
Identities = 20/38 (52%), Positives = 26/38 (68%)
Frame = +1 / +1
Query: 178 ITAILSKAGQFTKFLQLLQSTREAEQITNQLKGKPSNG 291
IT +L+KAGQF F++LL+ST A QI NQL +G
Sbjct: 166 ITGVLAKAGQFNTFIRLLKSTGVAAQIDNQLNNSFGSG 279
>gb|BI959905.1|BI959905 HVSMEn0022F07f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0022F07f, mRNA sequence
Length = 646
Score = 110 bits (235), Expect(2) = 1e-031
Identities = 43/71 (60%), Positives = 59/71 (83%)
Frame = +1 / +2
Query: 292 GLTVFAPPDSAFSALPAGTLNSLSDQQKTSLVQFHVVSAALPAAQLETVSNPLRTQAGDT 471
G+TVFAP D+AF++L +GTLNSLSD+QK +L+Q+HV+S +P +Q +TVSNPL T AG +
Sbjct: 290 GMTVFAPTDNAFTSLASGTLNSLSDRQKNALIQYHVLSTGIPMSQFDTVSNPLLTHAGIS 469
Query: 472 GRGKYPLNLTA 504
G+YPLN+TA
Sbjct: 470 SPGQYPLNVTA 502
Score = 94.1 bits (199), Expect(2) = 5e-028
Identities = 43/71 (60%), Positives = 47/71 (66%)
Frame = -2 / -2
Query: 503 AVRLSGYLPLPVSPACVRSGLLTVSSCAAGSAADTTWNCTSDVFCWSERELSVPAGSAEK 324
AV LSGY P + PACVRSGLLTVS+C G + TW S FC S+ ELSVP S K
Sbjct: 501 AVTLSGYWPGELMPACVRSGLLTVSNCDMGMPVERTWYWISAFFCLSDSELSVPEASEVK 322
Query: 323 ALSGGANTVSP 291
ALS GANTV P
Sbjct: 321 ALSVGANTVMP 289
Score = 50.1 bits (103), Expect(2) = 5e-028
Identities = 22/38 (57%), Positives = 26/38 (68%)
Frame = -2 / -2
Query: 290 PLDGLPLSWLVICSASRVDCSSWRNLVNCPALLRIAVM 177
PL L LSWL IC+A+ VD SS N++NCPAL VM
Sbjct: 291 PLPKLLLSWLSICAATPVDLSSRMNVLNCPALASTPVM 178
Score = 46.0 bits (94), Expect(2) = 1e-031
Identities = 20/38 (52%), Positives = 26/38 (68%)
Frame = +1 / +2
Query: 178 ITAILSKAGQFTKFLQLLQSTREAEQITNQLKGKPSNG 291
IT +L+KAGQF F++LL+ST A QI NQL +G
Sbjct: 179 ITGVLAKAGQFNTFIRLLKSTGVAAQIDNQLNNSFGSG 292
>gb|BE455952.3|BE455952 HVSMEg0017N20f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0017N20f, mRNA sequence
Length = 799
Score = 106 bits (227), Expect = 1e-022
Identities = 45/95 (47%), Positives = 64/95 (67%)
Frame = +1 / +3
Query: 178 ITAILSKAGQFTKFLQLLQSTREAEQITNQLKGKPSNGGLTVFAPPDSAFSALPAGTLNS 357
+TAIL K GQ+T F++L++ T++ Q+ +QL + G TVFAP D+AF+ L GTLNS
Sbjct: 186 VTAILVKGGQYTTFIRLMKETQQDTQLNSQLNNSFNGNGYTVFAPTDNAFNNLKPGTLNS 365
Query: 358 LSDQQKTSLVQFHVVSAALPAAQLETVSNPLRTQA 462
LS Q++ SLVQ H++ +T SNP+RTQA
Sbjct: 366 LSQQEQVSLVQGHILPQYYSMESFQTASNPVRTQA 470
>gb|BM370974.2|BM370974 EBro04_SQ003_B17_R root, 3 week, salt-stressed, cv Optic, EBro04
Hordeum vulgare subsp. vulgare cDNA clone
EBro04_SQ003_B17 5', mRNA sequence
Length = 599
Score = 106 bits (227), Expect = 1e-022
Identities = 45/95 (47%), Positives = 64/95 (67%)
Frame = +1 / +1
Query: 178 ITAILSKAGQFTKFLQLLQSTREAEQITNQLKGKPSNGGLTVFAPPDSAFSALPAGTLNS 357
+TAIL K GQ+T F++L++ T++ Q+ +QL + G TVFAP D+AF+ L GTLNS
Sbjct: 172 VTAILVKGGQYTTFIRLMKETQQDTQLNSQLNNSFNGNGYTVFAPTDNAFNNLKPGTLNS 351
Query: 358 LSDQQKTSLVQFHVVSAALPAAQLETVSNPLRTQA 462
LS Q++ SLVQ H++ +T SNP+RTQA
Sbjct: 352 LSQQEQVSLVQGHILPQYYSMESFQTASNPVRTQA 456
>gb|BU978837.1|BU978837 HA14G17r HA Hordeum vulgare subsp. vulgare cDNA clone HA14G17
5-PRIME, mRNA sequence
Length = 514
Score = 106 bits (227), Expect = 1e-022
Identities = 45/95 (47%), Positives = 64/95 (67%)
Frame = +1 / +3
Query: 178 ITAILSKAGQFTKFLQLLQSTREAEQITNQLKGKPSNGGLTVFAPPDSAFSALPAGTLNS 357
+TAIL K GQ+T F++L++ T++ Q+ +QL + G TVFAP D+AF+ L GTLNS
Sbjct: 144 VTAILVKGGQYTTFIRLMKETQQDTQLNSQLNNSFNGNGYTVFAPTDNAFNNLKPGTLNS 323
Query: 358 LSDQQKTSLVQFHVVSAALPAAQLETVSNPLRTQA 462
LS Q++ SLVQ H++ +T SNP+RTQA
Sbjct: 324 LSQQEQVSLVQGHILPQYYSMESFQTASNPVRTQA 428
>gb|CA025009.1|CA025009 HZ50P08r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ50P08
5-PRIME, mRNA sequence
Length = 542
Score = 106 bits (227), Expect = 1e-022
Identities = 45/95 (47%), Positives = 64/95 (67%)
Frame = +1 / +1
Query: 178 ITAILSKAGQFTKFLQLLQSTREAEQITNQLKGKPSNGGLTVFAPPDSAFSALPAGTLNS 357
+TAIL K GQ+T F++L++ T++ Q+ +QL + G TVFAP D+AF+ L GTLNS
Sbjct: 196 VTAILVKGGQYTTFIRLMKETQQDTQLNSQLNNSFNGNGYTVFAPTDNAFNNLKPGTLNS 375
Query: 358 LSDQQKTSLVQFHVVSAALPAAQLETVSNPLRTQA 462
LS Q++ SLVQ H++ +T SNP+RTQA
Sbjct: 376 LSQQEQVSLVQGHILPQYYSMESFQTASNPVRTQA 480
>gb|BF628401.2|BF628401 HVSMEb0003O23f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0003O23f, mRNA sequence
Length = 497
Score = 97.7 bits (207), Expect(2) = 2e-022
Identities = 39/67 (58%), Positives = 51/67 (76%)
Frame = +1 / +3
Query: 292 GLTVFAPPDSAFSALPAGTLNSLSDQQKTSLVQFHVVSAALPAAQLETVSNPLRTQAGDT 471
G+TV AP D+AF++L GTL SLSD QK +L+Q+HV A+P +Q +TVSNPLRTQAG
Sbjct: 297 GMTVLAPTDNAFTSLAFGTLKSLSDSQKNALIQYHVRLTAIPMSQFDTVSNPLRTQAGSN 476
Query: 472 GRGKYPL 492
G++PL
Sbjct: 477 SPGQFPL 497
Score = 78.0 bits (164), Expect(2) = 5e-022
Identities = 38/69 (55%), Positives = 43/69 (62%)
Frame = -2 / -2
Query: 491 SGYLPLPVSPACVRSGLLTVSSCAAGSAADTTWNCTSDVFCWSERELSVPAGSAEKALSG 312
+G P + PACVRSGLLTVS+C G A TW S F S+ +LSVP S KALS
Sbjct: 496 NGNWPGELLPACVRSGLLTVSNCDMGMAVKRTWYWISAFFWLSDSDLSVPKASEVKALSV 317
Query: 311 GANTVSPPL 285
GANTV P L
Sbjct: 316 GANTVMPLL 290
Score = 46.0 bits (94), Expect(2) = 5e-022
Identities = 21/38 (55%), Positives = 25/38 (65%)
Frame = -2 / -2
Query: 290 PLDGLPLSWLVICSASRVDCSSWRNLVNCPALLRIAVM 177
PL L LSWL IC+A+ VD SS N++ CPAL VM
Sbjct: 298 PLLKLLLSWLSICAATPVDLSSRMNVLKCPALASTPVM 185
Score = 27.6 bits (54), Expect(2) = 2e-022
Identities = 13/38 (34%), Positives = 14/38 (36%)
Frame = +3 / +2
Query: 177 HHGDPEQGRAVHQXXXXXXXXXXXXXXHQPAQGQAVQR 290
HHG QGRA QPAQ Q +R
Sbjct: 185 HHGGAGQGRAFQHVHTAAQVDWRGGAYRQPAQQQLQER 298
>gb|BF254052.2|BF254052 HVSMEf0002N24f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0002N24f, mRNA sequence
Length = 867
Score = 103 bits (219), Expect = 1e-021
Identities = 44/95 (46%), Positives = 63/95 (66%)
Frame = +1 / +2
Query: 178 ITAILSKAGQFTKFLQLLQSTREAEQITNQLKGKPSNGGLTVFAPPDSAFSALPAGTLNS 357
+TAIL K GQ+T F++L++ T++ Q+ +QL + G TVFAP D+AF+ L GTLNS
Sbjct: 182 VTAILVKGGQYTTFIRLMKETQQDTQLNSQLNNSFNGNGYTVFAPTDNAFNNLKPGTLNS 361
Query: 358 LSDQQKTSLVQFHVVSAALPAAQLETVSNPLRTQA 462
LS ++ SLVQ H++ +T SNP+RTQA
Sbjct: 362 LSQHEQVSLVQGHILPQYYSMESFQTGSNPVRTQA 466
>gb|BU977817.1|BU977817 HA12B15r HA Hordeum vulgare subsp. vulgare cDNA clone HA12B15
5-PRIME, mRNA sequence
Length = 422
Score = 102 bits (218), Expect = 2e-021
Identities = 43/93 (46%), Positives = 62/93 (66%)
Frame = +1 / +3
Query: 178 ITAILSKAGQFTKFLQLLQSTREAEQITNQLKGKPSNGGLTVFAPPDSAFSALPAGTLNS 357
+TAIL K GQ+T F++L++ T++ Q+ +QL + G TVFAP D+AF+ L GTLNS
Sbjct: 144 VTAILVKGGQYTTFIRLMKETQQDTQLNSQLNNSFNGNGYTVFAPTDNAFNNLKPGTLNS 323
Query: 358 LSDQQKTSLVQFHVVSAALPAAQLETVSNPLRT 456
LS Q++ SLVQ H++ +T SNP+RT
Sbjct: 324 LSQQEQVSLVQGHILPQYYSMESFQTASNPVRT 422
>gb|BQ763227.1|BQ763227 EBro02_SQ007_J17_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ007_J17 5', mRNA sequence
Length = 581
Score = 78.5 bits (165), Expect(2) = 3e-021
Identities = 34/63 (53%), Positives = 42/63 (66%)
Frame = +1 / +2
Query: 283 SNGGLTVFAPPDSAFSALPAGTLNSLSDQQKTSLVQFHVVSAALPAAQLETVSNPLRTQA 462
S+ G TVFAP D+AF++L GTLNSLS Q + +LVQ H+V ET SNP+RTQA
Sbjct: 299 SDTGFTVFAPTDNAFNSLKPGTLNSLSQQDQVTLVQAHIVPTFYSMESFETASNPVRTQA 478
Query: 463 GDT 471
T
Sbjct: 479 SGT 487
Score = 42.8 bits (87), Expect(2) = 3e-021
Identities = 15/32 (46%), Positives = 25/32 (78%)
Frame = +1 / +2
Query: 172 KTITAILSKAGQFTKFLQLLQSTREAEQITNQ 267
+ +TA+L KAGQ+TKF++L+ ST++ Q+ Q
Sbjct: 194 RNVTAVLEKAGQYTKFIRLMASTQQDTQLNAQ 289
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 157,214,309
Number of Sequences: 312970
Number of extensions: 1794875
Number of successful extensions: 25406
Number of sequences better than 1.0e-020: 16
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 8440925091
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)