TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 8228901.3.1
(1173 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF065769.2|BF065769 HV_CEb0013O04f Hordeum vulgare seedl... 52 3e-027
gb|BE421410.1|BE421410 HWM009.A07 ITEC HWM Barley Leaf Libr... 53 5e-023
gb|BQ472388.1|BQ472388 HB09L20T BC Hordeum vulgare subsp. v... 50 3e-021
>gb|BF065769.2|BF065769 HV_CEb0013O04f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0013O04f, mRNA sequence
Length = 582
Score = 52.4 bits (108), Expect(4) = 3e-027
Identities = 19/38 (50%), Positives = 25/38 (65%)
Frame = +2 / +2
Query: 209 SALLPQTMVVADLGCSSGPNTLRFISEVIGIIARHCKE 322
+ LLP MV+ADLGCSSGPN + +S + I HC +
Sbjct: 200 TTLLPTKMVIADLGCSSGPNAVALVSTAVEAIHNHCHQ 313
Score = 47.8 bits (98), Expect(4) = 3e-027
Identities = 20/38 (52%), Positives = 25/38 (65%)
Frame = +2 / +2
Query: 488 LPGSFYTRIFPSESVHLFHSLFSVHWHSQASQQLKDTQ 601
LPGSFY R+F S S+HL S S+HW S+A +L Q
Sbjct: 446 LPGSFYERLFTSGSLHLVFSSNSLHWLSKAPDELTRNQ 559
Score = 46.9 bits (96), Expect(4) = 3e-027
Identities = 18/41 (43%), Positives = 27/41 (65%)
Frame = +2 / +2
Query: 71 MPMTIERDLHMATGNGETSYTKNSRIQEKVMFQIKPVLEEA 193
+ M E+ LHM G GETSY +NS +Q + ++KP++E A
Sbjct: 56 LAMAFEQKLHMKQGQGETSYARNSFVQNEGQKRMKPLIEAA 178
Score = 30.4 bits (60), Expect(4) = 3e-027
Identities = 13/29 (44%), Positives = 15/29 (51%)
Frame = +2 / +2
Query: 305 ARHCKEHDRRHDYPQLQFFLNDLPGNDFN 391
A H H P++ LNDLP NDFN
Sbjct: 290 AIHNHCHQFSQPPPEVSVLLNDLPDNDFN 376
>gb|BE421410.1|BE421410 HWM009.A07 ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM009.A07, mRNA sequence
Length = 802
Score = 52.8 bits (109), Expect(4) = 5e-023
Identities = 21/38 (55%), Positives = 27/38 (71%)
Frame = +2 / +3
Query: 488 LPGSFYTRIFPSESVHLFHSLFSVHWHSQASQQLKDTQ 601
+PGSFY R+FPS SVHLF S +HW S+A + L+ Q
Sbjct: 555 VPGSFYERLFPSGSVHLFCSSNGLHWLSKAPEDLRINQ 668
Score = 41.8 bits (85), Expect(4) = 5e-023
Identities = 17/44 (38%), Positives = 26/44 (59%)
Frame = +2 / +3
Query: 65 AAMPMTIERDLHMATGNGETSYTKNSRIQEKVMFQIKPVLEEAT 196
A+ M ++ +HM G G+TSY NS Q ++KP++E AT
Sbjct: 162 ASKAMAFKQMVHMNQGQGQTSYAHNSSFQSAEQNRMKPLIEAAT 293
Score = 40.9 bits (83), Expect(4) = 5e-023
Identities = 16/35 (45%), Positives = 21/35 (60%)
Frame = +2 / +3
Query: 209 SALLPQTMVVADLGCSSGPNTLRFISEVIGIIARH 313
+ L P MV+ADLGCSSGPN + +S + H
Sbjct: 315 TTLSPGKMVIADLGCSSGPNAVALVSIALEATRSH 419
Score = 27.2 bits (53), Expect(4) = 5e-023
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = +2 / +3
Query: 344 PQLQFFLNDLPGNDFN 391
P++ LNDLP NDFN
Sbjct: 444 PEVCVVLNDLPYNDFN 491
>gb|BQ472388.1|BQ472388 HB09L20T BC Hordeum vulgare subsp. vulgare cDNA clone HB09L20
5-PRIME, mRNA sequence
Length = 627
Score = 49.6 bits (102), Expect(4) = 3e-021
Identities = 20/33 (60%), Positives = 25/33 (75%)
Frame = +2 / +3
Query: 491 PGSFYTRIFPSESVHLFHSLFSVHWHSQASQQL 589
PGSFY R+F SESVHL S S+HW S+A ++L
Sbjct: 447 PGSFYERLFTSESVHLVCSSNSLHWLSKAPEEL 545
Score = 46.4 bits (95), Expect(4) = 3e-021
Identities = 19/34 (55%), Positives = 22/34 (64%)
Frame = +2 / +3
Query: 215 LLPQTMVVADLGCSSGPNTLRFISEVIGIIARHC 316
LL MV+ADLGCS+GPN L +S I I HC
Sbjct: 210 LLSGKMVIADLGCSTGPNALALVSIAIEAIHSHC 311
Score = 32.7 bits (65), Expect(4) = 3e-021
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +2 / +3
Query: 344 PQLQFFLNDLPGNDFNNL 397
P++ FLNDLP NDFN +
Sbjct: 333 PEVSVFLNDLPENDFNTV 386
Score = 27.6 bits (54), Expect(4) = 3e-021
Identities = 12/22 (54%), Positives = 13/22 (59%)
Frame = +2 / +2
Query: 101 MATGNGETSYTKNSRIQEKVMF 166
M G+GETSY NS IQ F
Sbjct: 86 MNQGHGETSYAHNSSIQNAEHF 151
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 649,506,014
Number of Sequences: 312970
Number of extensions: 11686201
Number of successful extensions: 71609
Number of sequences better than 1.0e-020: 4
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 14083489153
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)