TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 8010745.2.1
(805 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ463709.1|BJ463709 BJ463709 K. Sato unpublished cDNA li... 221 6e-087
gb|BQ468777.1|BQ468777 HM02F02r HM Hordeum vulgare subsp. v... 219 2e-077
gb|BJ474408.1|BJ474408 BJ474408 K. Sato unpublished cDNA li... 221 1e-071
gb|BJ473760.1|BJ473760 BJ473760 K. Sato unpublished cDNA li... 221 5e-071
gb|BU995809.1|BU995809 HM11G24r HM Hordeum vulgare subsp. v... 201 2e-070
gb|AV936469.1|AV936469 AV936469 K. Sato unpublished cDNA li... 221 2e-060
gb|CA018105.1|CA018105 HV07H15r HV Hordeum vulgare subsp. v... 216 1e-057
gb|BF623927.2|BF623927 HVSMEa0007M14f Hordeum vulgare seedl... 124 1e-056
gb|BJ475687.1|BJ475687 BJ475687 K. Sato unpublished cDNA li... 215 2e-055
gb|BJ467881.1|BJ467881 BJ467881 K. Sato unpublished cDNA li... 210 1e-053
gb|CB881964.1|CB881964 HM11G24w HM Hordeum vulgare subsp. v... 200 9e-051
gb|BE437689.1|BE437689 SFR006.D12F990705 ITEC SFR Barley Le... 84 3e-047
gb|BI958216.1|BI958216 HVSMEn0013O15f Hordeum vulgare rachi... 92 1e-045
gb|BF629833.2|BF629833 HVSMEb0007A10f Hordeum vulgare seedl... 126 3e-045
gb|BQ134683.1|BQ134683 LP30159 Lemma/palea-enriched cDNA li... 159 2e-038
gb|CB858437.1|CB858437 HI07A02w HI Hordeum vulgare subsp. v... 123 6e-035
gb|AL508271.1|AL508271 AL508271 Hordeum vulgare Barke devel... 86 4e-033
gb|BJ462494.1|BJ462494 BJ462494 K. Sato unpublished cDNA li... 114 3e-032
gb|AV920504.1|AV920504 AV920504 K. Sato unpublished cDNA li... 95 9e-032
gb|BJ467618.1|BJ467618 BJ467618 K. Sato unpublished cDNA li... 121 7e-030
gb|BQ755347.1|BQ755347 EBed07_SQ002_J07_R endosperm, 28 DPA... 121 1e-029
gb|BU986792.1|BU986792 HF12O16r HF Hordeum vulgare subsp. v... 121 1e-029
gb|BU984559.1|BU984559 HF04E16r HF Hordeum vulgare subsp. v... 121 3e-029
gb|AV832993.1|AV832993 AV832993 K. Sato unpublished cDNA li... 83 6e-029
gb|BI947748.1|BI947748 HVSMEl0006L16f Hordeum vulgare spike... 127 1e-028
gb|BI954140.1|BI954140 HVSMEm0016F19f Hordeum vulgare green... 79 2e-028
gb|BJ463795.1|BJ463795 BJ463795 K. Sato unpublished cDNA li... 110 3e-028
gb|BG301241.1|BG301241 HVSMEb0020C10f Hordeum vulgare seedl... 101 1e-027
gb|BE601818.2|BE601818 HVSMEh0099K11f Hordeum vulgare 5-45 ... 122 2e-027
gb|BI951308.1|BI951308 HVSMEl0025H09f Hordeum vulgare spike... 121 5e-027
gb|BQ466282.1|BQ466282 HT02B16r HT Hordeum vulgare subsp. v... 121 5e-027
gb|AV929747.1|AV929747 AV929747 K. Sato unpublished cDNA li... 83 8e-027
gb|BE601804.3|BE601804 HVSMEh0099J17f Hordeum vulgare 5-45 ... 117 6e-026
gb|BQ659818.1|BQ659818 HF01M24w HF Hordeum vulgare subsp. v... 117 6e-026
gb|BU977487.1|BU977487 HA11I05r HA Hordeum vulgare subsp. v... 77 1e-025
gb|BE422156.1|BE422156 HWM020cG.09r ITEC HWM Barley Leaf Li... 109 2e-025
gb|BG365882.1|BG365882 HVSMEi0004J22f Hordeum vulgare 20 DA... 110 2e-025
gb|AV918813.1|AV918813 AV918813 K. Sato unpublished cDNA li... 110 2e-025
gb|BJ467970.1|BJ467970 BJ467970 K. Sato unpublished cDNA li... 110 2e-025
gb|BQ659752.1|BQ659752 HF01J17w HF Hordeum vulgare subsp. v... 110 2e-025
gb|AV921900.1|AV921900 AV921900 K. Sato unpublished cDNA li... 110 2e-025
gb|BU972640.1|BU972640 HB22E11r BC Hordeum vulgare subsp. v... 108 2e-025
gb|BQ664794.1|BQ664794 HV04O11u HV Hordeum vulgare subsp. v... 109 3e-025
gb|BQ469152.1|BQ469152 HM03J01r HM Hordeum vulgare subsp. v... 108 3e-025
gb|BJ468579.1|BJ468579 BJ468579 K. Sato unpublished cDNA li... 110 4e-025
gb|CD662781.1|CD662781 UCRHV18_03ad10_b1 Drought-stressed D... 82 4e-025
gb|AV919161.1|AV919161 AV919161 K. Sato unpublished cDNA li... 108 6e-025
gb|BJ467710.1|BJ467710 BJ467710 K. Sato unpublished cDNA li... 108 6e-025
gb|AV917900.1|AV917900 AV917900 K. Sato unpublished cDNA li... 108 6e-025
gb|BJ468501.1|BJ468501 BJ468501 K. Sato unpublished cDNA li... 108 6e-025
gb|AV920604.1|AV920604 AV920604 K. Sato unpublished cDNA li... 108 6e-025
gb|BE454083.2|BE454083 HVSMEh0080E16f Hordeum vulgare 5-45 ... 113 1e-024
gb|AV917579.1|AV917579 AV917579 K. Sato unpublished cDNA li... 108 1e-024
gb|AV918128.1|AV918128 AV918128 K. Sato unpublished cDNA li... 108 1e-024
gb|AV918262.1|AV918262 AV918262 K. Sato unpublished cDNA li... 108 1e-024
gb|BJ466393.1|BJ466393 BJ466393 K. Sato unpublished cDNA li... 108 1e-024
gb|AV919977.1|AV919977 AV919977 K. Sato unpublished cDNA li... 107 1e-024
gb|BE601810.2|BE601810 HVSMEh0099K03f Hordeum vulgare 5-45 ... 112 3e-024
gb|BI949909.1|BI949909 HVSMEl0016O06f Hordeum vulgare spike... 111 4e-024
gb|CV053661.1|CV053661 BNEL101g2 Barley EST endosperm libra... 111 4e-024
gb|CK124362.1|CK124362 BES1824108i03 BES1824 Hordeum vulgar... 111 4e-024
gb|AV912606.1|AV912606 AV912606 K. Sato unpublished cDNA li... 104 1e-023
gb|BG365441.1|BG365441 HVSMEi0002L08f Hordeum vulgare 20 DA... 109 2e-023
gb|BQ468553.1|BQ468553 HM01J08T HM Hordeum vulgare subsp. v... 109 2e-023
gb|BU993981.1|BU993981 HM05D21r HM Hordeum vulgare subsp. v... 109 2e-023
gb|CV054913.1|CV054913 BNEL114f9 Barley EST endosperm libra... 109 2e-023
gb|BJ459769.1|BJ459769 BJ459769 K. Sato unpublished cDNA li... 105 2e-023
gb|BJ458822.1|BJ458822 BJ458822 K. Sato unpublished cDNA li... 105 2e-023
gb|BJ473754.1|BJ473754 BJ473754 K. Sato unpublished cDNA li... 105 2e-023
gb|BF630667.2|BF630667 HVSMEb0012M20f Hordeum vulgare seedl... 105 2e-023
gb|AV928001.1|AV928001 AV928001 K. Sato unpublished cDNA li... 83 3e-023
gb|BI947342.1|BI947342 HVSMEl0005D04f Hordeum vulgare spike... 108 3e-023
gb|AV918243.1|AV918243 AV918243 K. Sato unpublished cDNA li... 108 3e-023
gb|AV918260.1|AV918260 AV918260 K. Sato unpublished cDNA li... 108 3e-023
gb|AV919042.1|AV919042 AV919042 K. Sato unpublished cDNA li... 108 3e-023
gb|AV921410.1|AV921410 AV921410 K. Sato unpublished cDNA li... 108 3e-023
gb|BJ465763.1|BJ465763 BJ465763 K. Sato unpublished cDNA li... 108 3e-023
gb|BQ657405.1|BQ657405 HA08I10u HA Hordeum vulgare subsp. v... 108 3e-023
gb|BM375888.2|BM375888 EBem06_SQ004_L04_R embryo, 21 DPA, n... 108 3e-023
gb|CA028155.1|CA028155 HZ61C14r HZ Hordeum vulgare subsp. v... 108 3e-023
gb|CB880505.1|CB880505 HM06L21w HM Hordeum vulgare subsp. v... 108 3e-023
gb|AV912380.1|AV912380 AV912380 K. Sato unpublished cDNA li... 83 3e-023
gb|AV917200.1|AV917200 AV917200 K. Sato unpublished cDNA li... 108 5e-023
gb|BM373002.2|BM373002 EBma04_SQ002_L05_R maternal, 10 DPA,... 67 8e-023
gb|CB858315.1|CB858315 HI06J05w HI Hordeum vulgare subsp. v... 101 9e-023
gb|CA000318.1|CA000318 HS09A03u HS Hordeum vulgare subsp. v... 101 9e-023
gb|AV913933.1|AV913933 AV913933 K. Sato unpublished cDNA li... 107 9e-023
gb|AV921547.1|AV921547 AV921547 K. Sato unpublished cDNA li... 107 9e-023
gb|AV921892.1|AV921892 AV921892 K. Sato unpublished cDNA li... 107 9e-023
gb|BJ468274.1|BJ468274 BJ468274 K. Sato unpublished cDNA li... 107 9e-023
gb|BQ661453.1|BQ661453 HM03J01u HM Hordeum vulgare subsp. v... 107 9e-023
gb|BG417583.2|BG417583 HVSMEk0018E23f Hordeum vulgare testa... 82 1e-022
gb|AV917652.1|AV917652 AV917652 K. Sato unpublished cDNA li... 100 2e-022
gb|BJ545510.1|BJ545510 BJ545510 K. Sato unpublished cDNA li... 83 3e-022
gb|BF253999.2|BF253999 HVSMEf0002L11f Hordeum vulgare seedl... 105 3e-022
gb|BE420971.1|BE420971 HWM004.D12 ITEC HWM Barley Leaf Libr... 105 4e-022
gb|BE196548.3|BE196548 HVSMEh0092J16f Hordeum vulgare 5-45 ... 105 4e-022
gb|BM444610.1|BM444610 EBem09_SQ005_O09_R embryo, 1 Day ger... 104 6e-022
gb|CB880642.1|CB880642 HM07D21w HM Hordeum vulgare subsp. v... 102 2e-021
gb|BQ764256.1|BQ764256 EBan01_SQ005_G11_R anther, yellow st... 102 3e-021
gb|BQ766937.1|BQ766937 EBro08_SQ007_D17_R root, 3 week, dro... 77 3e-021
gb|BM373123.2|BM373123 EBma04_SQ003_D03_R maternal, 10 DPA,... 62 3e-021
gb|BE231065.1|BE231065 HVSMEg0016D19f Hordeum vulgare pre-a... 77 3e-021
gb|BF263505.3|BF263505 HV_CEa0006O18f Hordeum vulgare seedl... 83 3e-021
gb|CB882027.1|CB882027 HM11K09w HM Hordeum vulgare subsp. v... 101 4e-021
gb|CA006118.1|CA006118 HU05H21u HU Hordeum vulgare subsp. v... 95 5e-021
gb|BF261097.2|BF261097 HVSMEf0023M01f Hordeum vulgare seedl... 95 7e-021
>gb|BJ463709.1|BJ463709 BJ463709 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30c23 5', mRNA sequence
Length = 688
Score = 221 bits (477), Expect(3) = 6e-087
Identities = 82/130 (63%), Positives = 107/130 (82%)
Frame = -2 / +2
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
DGV IG TA G++DVPE +EL ++YVN RY+ PVY+TENG+ Q+SD S E+LI+DV
Sbjct: 248 DGVAIGRPTAFPGYYDVPEGMELIVKYVNQRYENAPVYVTENGFSQFSDNSMEDLINDVG 427
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRM 187
R NYLQGY+T +S A+R GANVRGYFVW+L+DNFEW FG+ +++GLY+VDF+TQERT +M
Sbjct: 428 RVNYLQGYLTCISSAVRRGANVRGYFVWSLMDNFEWGFGFTVRFGLYYVDFETQERTPKM 607
Query: 186 SARWYQGFLT 157
S +WY+ FLT
Sbjct: 608 SGKWYRDFLT 637
Score = 126 bits (269), Expect(3) = 2e-039
Identities = 64/122 (52%), Positives = 71/122 (58%)
Frame = +2 / -2
Query: 158 VRKPWYHLADIRXVLSWVSKSTWYSPYFNL*PNAHSKLSSSVHTK*PRTFAPFLSALDRY 337
VRK YH DI VLS VSKST YSP + P HSKLS +HTK*PRTFAP L+A + +
Sbjct: 636 VRKSRYHFPDIFGVLSCVSKST*YSPNLTVKPKPHSKLSMRLHTK*PRTFAPLLTAEEMH 457
Query: 338 VM*PCR*FFXXXXXXXXXXXXXXHCX*PFSVIYTGVPLYLPFTYWIASSMASGTSKKPAR 517
V *PCR*F +C PFSV YTG YL TY SS+ SGTS P
Sbjct: 456 VR*PCR*FTLPTSLIKSSILLSLNCEKPFSVTYTGAFSYLWLTYLTISSIPSGTS**PGN 277
Query: 518 AV 523
AV
Sbjct: 276 AV 271
Score = 106 bits (227), Expect(2) = 5e-027
Identities = 60/113 (53%), Positives = 72/113 (63%)
Frame = -1 / +1
Query: 493 RGHRAGYPVREREVQGHACVYY*ERLLAVE*CQQGGADRRCEEEELPAGLHHVSIQSTQE 314
R + A V + E++ A V Y*+ LLAV+ Q GG D+ + ELPAGL V S+QE
Sbjct: 301 RRNGADRQVCQPEIRERAGVRY*KWLLAVQR*QYGGLDQ*RWKSELPAGLSDVHFFSSQE 480
Query: 313 RSKRAGLLRVDAAGQLRVGVRLQVEVRAVPRXLRHPGEDAPDVCEVVPGLPHG 155
RSKRA LLRV+ GQLRVG L EVRAV R + GED+ DV EVVP L HG
Sbjct: 481 RSKRAWLLRVEPHGQLRVGFWLHREVRAVLRGF*NAGEDSKDVGEVVPRLSHG 639
Score = 94.1 bits (199), Expect(2) = 5e-024
Identities = 42/96 (43%), Positives = 57/96 (59%)
Frame = +1 / -1
Query: 259 PLEVVQQRPHEVAPHVCSVPECFG*IRDVALQVVLPPHIVDQLLPAGITPLXVTVLSNIH 438
PLEVV + PHEVA HVCS P+C R + LQV+ +++DQ+L I L + SN+H
Sbjct: 535 PLEVVHEAPHEVATHVCSSPDC*RNARQITLQVIHSSNVIDQVLHTVIAELREAIFSNVH 356
Query: 439 RRALVPPVHVLDSQLYGLWDVEETCQSSISPDLDPV 546
RR LV V +LD QL+ ++ T + S S D V
Sbjct: 355 RRVLVSLVDILDDQLHSFGNIIVTRECSWSSDCHAV 248
Score = 91.3 bits (193), Expect(3) = 6e-087
Identities = 35/49 (71%), Positives = 41/49 (83%)
Frame = -2 / +2
Query: 693 EKALLLRYKADFIGLNHYTAIYARDCLRSPCNLGSYEGNAFVSATGERD 547
E+ LL+ KADFIG+NHYTAIYA+DC+ SPCN+ SYEGNA V A GERD
Sbjct: 104 EEKRLLQSKADFIGVNHYTAIYAKDCIASPCNIKSYEGNALVQAVGERD 250
Score = 47.8 bits (98), Expect(3) = 2e-039
Identities = 23/33 (69%), Positives = 25/33 (75%)
Frame = +2 / -2
Query: 563 AETKAFPSYDPRLHGERRQSLA*IAV*WFSPMK 661
A T AFPS LHGE QSLA*IAV*WF+P+K
Sbjct: 234 AWTSAFPS*LLMLHGEAMQSLA*IAV*WFTPIK 136
Score = 47.8 bits (98), Expect(3) = 6e-087
Identities = 19/26 (73%), Positives = 22/26 (84%)
Frame = -2 / +2
Query: 756 GDYPGAMREILGSDLPTFTAEEKALL 679
GDYP MRE+L S+LPTFT+EEK LL
Sbjct: 44 GDYPREMREMLSSNLPTFTSEEKRLL 121
Score = 36.8 bits (74), Expect(2) = 5e-024
Identities = 17/38 (44%), Positives = 23/38 (60%)
Frame = +1 / -1
Query: 547 VPFPGRRDEGVPFVRPEVARGAQAVPGVDRRVVVQPDE 660
V F D+ + V VAR + A+ GVDRRV+V PD+
Sbjct: 250 VSFTYSLDQRISLVTLNVARRSDAILGVDRRVMVYPDK 137
Score = 34.1 bits (68), Expect(2) = 5e-027
Identities = 13/20 (65%), Positives = 15/20 (75%)
Frame = -1 / +1
Query: 658 HRAEPLHGDLRQGLPALPVQ 599
+R +PLHGDLRQGL VQ
Sbjct: 139 YRGKPLHGDLRQGLHRFSVQ 198
Score = 27.2 bits (53), Expect(3) = 2e-039
Identities = 12/20 (60%), Positives = 15/20 (75%)
Frame = +2 / -2
Query: 701 VKVGRSDPRISRIAPG*SPR 760
V VG+ D ISRI+ G*SP+
Sbjct: 99 VNVGKFDDSISRISLG*SPK 40
>gb|BQ468777.1|BQ468777 HM02F02r HM Hordeum vulgare subsp. vulgare cDNA clone HM02F02
5-PRIME, mRNA sequence
Length = 553
Score = 219 bits (473), Expect(2) = 2e-077
Identities = 81/130 (62%), Positives = 107/130 (82%)
Frame = -2 / +1
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
DGV IG TA G++DVPE +EL ++YVN RY+ PVY+TENG+ Q+SD S ++LI+DV
Sbjct: 148 DGVAIGRPTAFPGYYDVPEGMELIVKYVNQRYENAPVYVTENGFSQFSDNSMKDLINDVG 327
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRM 187
R NYLQGY+T +S A+R GANVRGYFVW+L+DNFEW FG+ +++GLY+VDF+TQERT +M
Sbjct: 328 RVNYLQGYLTCISSAVRRGANVRGYFVWSLMDNFEWGFGFTVRFGLYYVDFETQERTPKM 507
Query: 186 SARWYQGFLT 157
S +WY+ FLT
Sbjct: 508 SGKWYRDFLT 537
Score = 127 bits (272), Expect(2) = 3e-037
Identities = 65/122 (53%), Positives = 71/122 (58%)
Frame = +2 / -3
Query: 158 VRKPWYHLADIRXVLSWVSKSTWYSPYFNL*PNAHSKLSSSVHTK*PRTFAPFLSALDRY 337
VRK YH DI VLS VSKST YSP + P HSKLS +HTK*PRTFAP L+A + +
Sbjct: 536 VRKSRYHFPDIFGVLSCVSKST*YSPNLTVKPKPHSKLSMRLHTK*PRTFAPLLTAEEMH 357
Query: 338 VM*PCR*FFXXXXXXXXXXXXXXHCX*PFSVIYTGVPLYLPFTYWIASSMASGTSKKPAR 517
V *PCR*F +C PFSV YTG YL TY SSM SGTS P
Sbjct: 356 VR*PCR*FTLPTSLIKSFILLSLNCEKPFSVTYTGAFSYLWLTYLTISSMPSGTS**PGN 177
Query: 518 AV 523
AV
Sbjct: 176 AV 171
Score = 110 bits (234), Expect(2) = 6e-028
Identities = 60/113 (53%), Positives = 73/113 (64%)
Frame = -1 / +3
Query: 493 RGHRAGYPVREREVQGHACVYY*ERLLAVE*CQQGGADRRCEEEELPAGLHHVSIQSTQE 314
R H A V + E++ A V Y*+ LLAV+ Q G D+ + ELPAGL + + S+QE
Sbjct: 201 RRHGADRQVCQPEIRERAGVRY*KWLLAVQR*QYEGLDQ*RWKSELPAGLSDMHLFSSQE 380
Query: 313 RSKRAGLLRVDAAGQLRVGVRLQVEVRAVPRXLRHPGEDAPDVCEVVPGLPHG 155
RSKRA LLRV+ GQLRVG L EVRAV R R+ GED+ DV EVVP L HG
Sbjct: 381 RSKRAWLLRVEPHGQLRVGFWLHREVRAVLRGFRNAGEDSKDVGEVVPRLSHG 539
Score = 90.4 bits (191), Expect(2) = 6e-023
Identities = 41/96 (42%), Positives = 56/96 (58%)
Frame = +1 / -2
Query: 259 PLEVVQQRPHEVAPHVCSVPECFG*IRDVALQVVLPPHIVDQLLPAGITPLXVTVLSNIH 438
PLEVV + PHEVA HVCS P+C + LQV+ +++DQ+L I L + SN+H
Sbjct: 435 PLEVVHEAPHEVATHVCSSPDC*RDACQITLQVIHSSNVIDQVLHTVIAELREAIFSNVH 256
Query: 439 RRALVPPVHVLDSQLYGLWDVEETCQSSISPDLDPV 546
RR LV V +LD QL+ ++ T + S S D V
Sbjct: 255 RRVLVSLVDILDDQLHAFGNIIVTRECSWSSDCHAV 148
Score = 90.0 bits (190), Expect(2) = 2e-077
Identities = 34/45 (75%), Positives = 39/45 (86%)
Frame = -2 / +1
Query: 681 LLRYKADFIGLNHYTAIYARDCLRSPCNLGSYEGNAFVSATGERD 547
LL+ KADFIG+NHYTAIYA+DC+ SPCN+ SYEGNA V A GERD
Sbjct: 16 LLQSKADFIGVNHYTAIYAKDCIASPCNIKSYEGNALVQAVGERD 150
Score = 47.8 bits (98), Expect(2) = 3e-037
Identities = 23/33 (69%), Positives = 25/33 (75%)
Frame = +2 / -3
Query: 563 AETKAFPSYDPRLHGERRQSLA*IAV*WFSPMK 661
A T AFPS LHGE QSLA*IAV*WF+P+K
Sbjct: 134 AWTSAFPS*LLMLHGEAMQSLA*IAV*WFTPIK 36
Score = 36.8 bits (74), Expect(2) = 6e-023
Identities = 17/38 (44%), Positives = 23/38 (60%)
Frame = +1 / -2
Query: 547 VPFPGRRDEGVPFVRPEVARGAQAVPGVDRRVVVQPDE 660
V F D+ + V VAR + A+ GVDRRV+V PD+
Sbjct: 150 VSFTYSLDQRISLVTLNVARRSDAILGVDRRVMVYPDK 37
Score = 34.1 bits (68), Expect(2) = 6e-028
Identities = 13/20 (65%), Positives = 15/20 (75%)
Frame = -1 / +3
Query: 658 HRAEPLHGDLRQGLPALPVQ 599
+R +PLHGDLRQGL VQ
Sbjct: 39 YRGKPLHGDLRQGLHRFSVQ 98
>gb|BJ474408.1|BJ474408 BJ474408 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal13k12 3', mRNA sequence
Length = 769
Score = 221 bits (477), Expect(2) = 1e-071
Identities = 82/130 (63%), Positives = 107/130 (82%)
Frame = -2 / -3
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
DGV IG TA G++DVPE +EL ++YVN RY+ PVY+TENG+ Q+SD S E+LI+DV
Sbjct: 668 DGVAIGRPTAFPGYYDVPEGMELIVKYVNQRYENAPVYVTENGFSQFSDNSMEDLINDVG 489
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRM 187
R NYLQGY+T +S A+R GANVRGYFVW+L+DNFEW FG+ +++GLY+VDF+TQERT +M
Sbjct: 488 RVNYLQGYLTCISSAVRRGANVRGYFVWSLMDNFEWGFGFTVRFGLYYVDFETQERTPKM 309
Query: 186 SARWYQGFLT 157
S +WY+ FLT
Sbjct: 308 SGKWYRDFLT 279
Score = 126 bits (269), Expect(2) = 1e-033
Identities = 64/122 (52%), Positives = 71/122 (58%)
Frame = +2 / +1
Query: 158 VRKPWYHLADIRXVLSWVSKSTWYSPYFNL*PNAHSKLSSSVHTK*PRTFAPFLSALDRY 337
VRK YH DI VLS VSKST YSP + P HSKLS +HTK*PRTFAP L+A + +
Sbjct: 280 VRKSRYHFPDIFGVLSCVSKST*YSPNLTVKPKPHSKLSMRLHTK*PRTFAPLLTAEEMH 459
Query: 338 VM*PCR*FFXXXXXXXXXXXXXXHCX*PFSVIYTGVPLYLPFTYWIASSMASGTSKKPAR 517
V *PCR*F +C PFSV YTG YL TY SS+ SGTS P
Sbjct: 460 VR*PCR*FTLPTSLIKSSILLSLNCEKPFSVTYTGAFSYLWLTYLTISSIPSGTS**PGN 639
Query: 518 AV 523
AV
Sbjct: 640 AV 645
Score = 106 bits (227), Expect(2) = 5e-026
Identities = 60/113 (53%), Positives = 72/113 (63%)
Frame = -1 / -2
Query: 493 RGHRAGYPVREREVQGHACVYY*ERLLAVE*CQQGGADRRCEEEELPAGLHHVSIQSTQE 314
R + A V + E++ A V Y*+ LLAV+ Q GG D+ + ELPAGL V S+QE
Sbjct: 615 RRNGADRQVCQPEIRERAGVRY*KWLLAVQR*QYGGLDQ*RWKSELPAGLSDVHFFSSQE 436
Query: 313 RSKRAGLLRVDAAGQLRVGVRLQVEVRAVPRXLRHPGEDAPDVCEVVPGLPHG 155
RSKRA LLRV+ GQLRVG L EVRAV R + GED+ DV EVVP L HG
Sbjct: 435 RSKRAWLLRVEPHGQLRVGFWLHREVRAVLRGF*NAGEDSKDVGEVVPRLSHG 277
Score = 94.1 bits (199), Expect(2) = 1e-022
Identities = 42/96 (43%), Positives = 57/96 (59%)
Frame = +1 / +3
Query: 259 PLEVVQQRPHEVAPHVCSVPECFG*IRDVALQVVLPPHIVDQLLPAGITPLXVTVLSNIH 438
PLEVV + PHEVA HVCS P+C R + LQV+ +++DQ+L I L + SN+H
Sbjct: 381 PLEVVHEAPHEVATHVCSSPDC*RNARQITLQVIHSSNVIDQVLHTVIAELREAIFSNVH 560
Query: 439 RRALVPPVHVLDSQLYGLWDVEETCQSSISPDLDPV 546
RR LV V +LD QL+ ++ T + S S D V
Sbjct: 561 RRVLVSLVDILDDQLHSFGNIIVTRECSWSSDCHAV 668
Score = 68.9 bits (144), Expect(2) = 1e-071
Identities = 25/34 (73%), Positives = 28/34 (82%)
Frame = -2 / -3
Query: 648 NHYTAIYARDCLRSPCNLGSYEGNAFVSATGERD 547
NHYTAIYA+DC+ SPCN+ SYE NA V A GERD
Sbjct: 767 NHYTAIYAKDCIASPCNIKSYEXNALVQAVGERD 666
Score = 37.3 bits (75), Expect(2) = 1e-033
Identities = 20/29 (68%), Positives = 20/29 (68%)
Frame = +2 / +1
Query: 563 AETKAFPSYDPRLHGERRQSLA*IAV*WF 649
A T AF S LHGE QSLA*IAV*WF
Sbjct: 682 AWTSAFXS*LLMLHGEAMQSLA*IAV*WF 768
Score = 32.2 bits (64), Expect(2) = 1e-022
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = +3 / +2
Query: 600 CTGSAGSPWRRSPCSG 647
CT +PWRRSPC+G
Sbjct: 719 CTEKRCNPWRRSPCNG 766
Score = 30.9 bits (61), Expect(2) = 5e-026
Identities = 12/17 (70%), Positives = 13/17 (76%)
Frame = -1 / -2
Query: 649 EPLHGDLRQGLPALPVQ 599
+PLHGDLRQGL VQ
Sbjct: 768 KPLHGDLRQGLHRFSVQ 718
>gb|BJ473760.1|BJ473760 BJ473760 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal16n11 3', mRNA sequence
Length = 764
Score = 221 bits (477), Expect(2) = 5e-071
Identities = 82/130 (63%), Positives = 107/130 (82%)
Frame = -2 / -3
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
DGV IG TA G++DVPE +EL ++YVN RY+ PVY+TENG+ Q+SD S E+LI+DV
Sbjct: 666 DGVAIGRPTAFPGYYDVPEGMELIVKYVNQRYENAPVYVTENGFSQFSDNSMEDLINDVG 487
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRM 187
R NYLQGY+T +S A+R GANVRGYFVW+L+DNFEW FG+ +++GLY+VDF+TQERT +M
Sbjct: 486 RVNYLQGYLTCISSAVRRGANVRGYFVWSLMDNFEWGFGFTVRFGLYYVDFETQERTPKM 307
Query: 186 SARWYQGFLT 157
S +WY+ FLT
Sbjct: 306 SGKWYRDFLT 277
Score = 126 bits (269), Expect(2) = 2e-033
Identities = 64/122 (52%), Positives = 71/122 (58%)
Frame = +2 / +2
Query: 158 VRKPWYHLADIRXVLSWVSKSTWYSPYFNL*PNAHSKLSSSVHTK*PRTFAPFLSALDRY 337
VRK YH DI VLS VSKST YSP + P HSKLS +HTK*PRTFAP L+A + +
Sbjct: 278 VRKSRYHFPDIFGVLSCVSKST*YSPNLTVKPKPHSKLSMRLHTK*PRTFAPLLTAEEMH 457
Query: 338 VM*PCR*FFXXXXXXXXXXXXXXHCX*PFSVIYTGVPLYLPFTYWIASSMASGTSKKPAR 517
V *PCR*F +C PFSV YTG YL TY SS+ SGTS P
Sbjct: 458 VR*PCR*FTLPTSLIKSSILLSLNCEKPFSVTYTGAFSYLWLTYLTISSIPSGTS**PGN 637
Query: 518 AV 523
AV
Sbjct: 638 AV 643
Score = 106 bits (227), Expect(2) = 1e-024
Identities = 60/113 (53%), Positives = 72/113 (63%)
Frame = -1 / -2
Query: 493 RGHRAGYPVREREVQGHACVYY*ERLLAVE*CQQGGADRRCEEEELPAGLHHVSIQSTQE 314
R + A V + E++ A V Y*+ LLAV+ Q GG D+ + ELPAGL V S+QE
Sbjct: 613 RRNGADRQVCQPEIRERAGVRY*KWLLAVQR*QYGGLDQ*RWKSELPAGLSDVHFFSSQE 434
Query: 313 RSKRAGLLRVDAAGQLRVGVRLQVEVRAVPRXLRHPGEDAPDVCEVVPGLPHG 155
RSKRA LLRV+ GQLRVG L EVRAV R + GED+ DV EVVP L HG
Sbjct: 433 RSKRAWLLRVEPHGQLRVGFWLHREVRAVLRGF*NAGEDSKDVGEVVPRLSHG 275
Score = 94.1 bits (199), Expect(2) = 4e-021
Identities = 42/96 (43%), Positives = 57/96 (59%)
Frame = +1 / +1
Query: 259 PLEVVQQRPHEVAPHVCSVPECFG*IRDVALQVVLPPHIVDQLLPAGITPLXVTVLSNIH 438
PLEVV + PHEVA HVCS P+C R + LQV+ +++DQ+L I L + SN+H
Sbjct: 379 PLEVVHEAPHEVATHVCSSPDC*RNARQITLQVIHSSNVIDQVLHTVIAELREAIFSNVH 558
Query: 439 RRALVPPVHVLDSQLYGLWDVEETCQSSISPDLDPV 546
RR LV V +LD QL+ ++ T + S S D V
Sbjct: 559 RRVLVSLVDILDDQLHSFGNIIVTRECSWSSDCHAV 666
Score = 67.0 bits (140), Expect(2) = 5e-071
Identities = 24/33 (72%), Positives = 27/33 (81%)
Frame = -2 / -3
Query: 645 HYTAIYARDCLRSPCNLGSYEGNAFVSATGERD 547
HY AIYA+DC+ SPCN+ SYEGNA V A GERD
Sbjct: 762 HYXAIYAKDCIASPCNIKSYEGNALVQAVGERD 664
Score = 36.3 bits (73), Expect(2) = 2e-033
Identities = 19/28 (67%), Positives = 19/28 (67%)
Frame = +2 / +2
Query: 563 AETKAFPSYDPRLHGERRQSLA*IAV*W 646
A T AFPS LHGE QSLA*IA *W
Sbjct: 680 AWTSAFPS*LLMLHGEAMQSLA*IAX*W 763
Score = 27.2 bits (53), Expect(2) = 4e-021
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +3 / +3
Query: 600 CTGSAGSPWRRSPCSG 647
CT +PWRRSP +G
Sbjct: 717 CTEKRCNPWRRSPXNG 764
Score = 26.3 bits (51), Expect(2) = 1e-024
Identities = 11/16 (68%), Positives = 11/16 (68%)
Frame = -1 / -2
Query: 646 PLHGDLRQGLPALPVQ 599
PL GDLRQGL VQ
Sbjct: 763 PLXGDLRQGLHRFSVQ 716
>gb|BU995809.1|BU995809 HM11G24r HM Hordeum vulgare subsp. vulgare cDNA clone HM11G24
5-PRIME, mRNA sequence
Length = 630
Score = 201 bits (434), Expect(2) = 2e-070
Identities = 75/120 (62%), Positives = 99/120 (82%)
Frame = -2 / +2
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
DGV IG TA G++DVPE +EL ++YVN RY+ PVY+TENG+ Q+SD S ++LI+DV
Sbjct: 143 DGVAIGRPTAFPGYYDVPEGMELIVKYVNQRYENAPVYVTENGFSQFSDNSMKDLINDVG 322
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRM 187
R NYLQGY+T +S A+R GANVRGYFVW+L+DNFEW FG+ +++GLY+VDF+TQERT +M
Sbjct: 323 RVNYLQGYLTCISSAVRRGANVRGYFVWSLMDNFEWGFGFTVRFGLYYVDFETQERTPKM 502
Score = 113 bits (242), Expect(2) = 4e-033
Identities = 58/109 (53%), Positives = 64/109 (58%)
Frame = +2 / -1
Query: 197 VLSWVSKSTWYSPYFNL*PNAHSKLSSSVHTK*PRTFAPFLSALDRYVM*PCR*FFXXXX 376
VLS VSKST YSP + P HSKLS +HTK*PRTFAP L+A + +V *PCR*F
Sbjct: 492 VLSCVSKST*YSPNLTVKPKPHSKLSMRLHTK*PRTFAPLLTAEEMHVR*PCR*FTLPTS 313
Query: 377 XXXXXXXXXXHCX*PFSVIYTGVPLYLPFTYWIASSMASGTSKKPARAV 523
+C PFSV YTG YL TY SSM SGTS P AV
Sbjct: 312 LIKSFILLSLNCEKPFSVTYTGAFSYLWLTYLTISSMPSGTS**PGNAV 166
Score = 93.6 bits (198), Expect(2) = 5e-023
Identities = 52/102 (50%), Positives = 65/102 (63%)
Frame = -1 / +1
Query: 493 RGHRAGYPVREREVQGHACVYY*ERLLAVE*CQQGGADRRCEEEELPAGLHHVSIQSTQE 314
R H A V + E++ A V Y*+ LLAV+ Q G D+ + ELPAGL + + S+QE
Sbjct: 196 RRHGADRQVCQPEIRERAGVRY*KWLLAVQR*QYEGLDQ*RWKSELPAGLSDMHLFSSQE 375
Query: 313 RSKRAGLLRVDAAGQLRVGVRLQVEVRAVPRXLRHPGEDAPD 188
RSKRA LLRV+ GQLRVG L EVRAV R R+ GED+ D
Sbjct: 376 RSKRAWLLRVEPHGQLRVGFWLHREVRAVLRGFRNAGEDSKD 501
Score = 90.4 bits (191), Expect(2) = 6e-023
Identities = 41/96 (42%), Positives = 56/96 (58%)
Frame = +1 / -3
Query: 259 PLEVVQQRPHEVAPHVCSVPECFG*IRDVALQVVLPPHIVDQLLPAGITPLXVTVLSNIH 438
PLEVV + PHEVA HVCS P+C + LQV+ +++DQ+L I L + SN+H
Sbjct: 430 PLEVVHEAPHEVATHVCSSPDC*RDACQITLQVIHSSNVIDQVLHTVIAELREAIFSNVH 251
Query: 439 RRALVPPVHVLDSQLYGLWDVEETCQSSISPDLDPV 546
RR LV V +LD QL+ ++ T + S S D V
Sbjct: 250 RRVLVSLVDILDDQLHAFGNIIVTRECSWSSDCHAV 143
Score = 84.9 bits (179), Expect(2) = 2e-070
Identities = 31/41 (75%), Positives = 36/41 (87%)
Frame = -2 / +2
Query: 669 KADFIGLNHYTAIYARDCLRSPCNLGSYEGNAFVSATGERD 547
+ADFIG+NHYTAIYA+DC+ SPCN+ SYEGNA V A GERD
Sbjct: 23 QADFIGVNHYTAIYAKDCIASPCNIKSYEGNALVQAVGERD 145
Score = 47.8 bits (98), Expect(2) = 4e-033
Identities = 23/33 (69%), Positives = 25/33 (75%)
Frame = +2 / -1
Query: 563 AETKAFPSYDPRLHGERRQSLA*IAV*WFSPMK 661
A T AFPS LHGE QSLA*IAV*WF+P+K
Sbjct: 129 AWTSAFPS*LLMLHGEAMQSLA*IAV*WFTPIK 31
Score = 36.8 bits (74), Expect(2) = 6e-023
Identities = 17/38 (44%), Positives = 23/38 (60%)
Frame = +1 / -3
Query: 547 VPFPGRRDEGVPFVRPEVARGAQAVPGVDRRVVVQPDE 660
V F D+ + V VAR + A+ GVDRRV+V PD+
Sbjct: 145 VSFTYSLDQRISLVTLNVARRSDAILGVDRRVMVYPDK 32
Score = 34.1 bits (68), Expect(2) = 5e-023
Identities = 13/20 (65%), Positives = 15/20 (75%)
Frame = -1 / +1
Query: 658 HRAEPLHGDLRQGLPALPVQ 599
+R +PLHGDLRQGL VQ
Sbjct: 34 YRGKPLHGDLRQGLHRFSVQ 93
>gb|AV936469.1|AV936469 AV936469 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal11g17 3', mRNA sequence
Length = 709
Score = 221 bits (477), Expect(2) = 2e-060
Identities = 82/130 (63%), Positives = 107/130 (82%)
Frame = -2 / -1
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
DGV IG TA G++DVPE +EL ++YVN RY+ PVY+TENG+ Q+SD S E+LI+DV
Sbjct: 667 DGVAIGRPTAFPGYYDVPEGMELIVKYVNQRYENAPVYVTENGFSQFSDNSMEDLINDVG 488
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRM 187
R NYLQGY+T +S A+R GANVRGYFVW+L+DNFEW FG+ +++GLY+VDF+TQERT +M
Sbjct: 487 RVNYLQGYLTCISSAVRRGANVRGYFVWSLMDNFEWGFGFTVRFGLYYVDFETQERTPKM 308
Query: 186 SARWYQGFLT 157
S +WY+ FLT
Sbjct: 307 SGKWYRDFLT 278
Score = 126 bits (269), Expect = 2e-028
Identities = 64/122 (52%), Positives = 71/122 (58%)
Frame = +2 / +3
Query: 158 VRKPWYHLADIRXVLSWVSKSTWYSPYFNL*PNAHSKLSSSVHTK*PRTFAPFLSALDRY 337
VRK YH DI VLS VSKST YSP + P HSKLS +HTK*PRTFAP L+A + +
Sbjct: 279 VRKSRYHFPDIFGVLSCVSKST*YSPNLTVKPKPHSKLSMRLHTK*PRTFAPLLTAEEMH 458
Query: 338 VM*PCR*FFXXXXXXXXXXXXXXHCX*PFSVIYTGVPLYLPFTYWIASSMASGTSKKPAR 517
V *PCR*F +C PFSV YTG YL TY SS+ SGTS P
Sbjct: 459 VR*PCR*FTLPTSLIKSSILLSLNCEKPFSVTYTGAFSYLWLTYLTISSIPSGTS**PGN 638
Query: 518 AV 523
AV
Sbjct: 639 AV 644
Score = 106 bits (227), Expect = 1e-022
Identities = 60/113 (53%), Positives = 72/113 (63%)
Frame = -1 / -3
Query: 493 RGHRAGYPVREREVQGHACVYY*ERLLAVE*CQQGGADRRCEEEELPAGLHHVSIQSTQE 314
R + A V + E++ A V Y*+ LLAV+ Q GG D+ + ELPAGL V S+QE
Sbjct: 614 RRNGADRQVCQPEIRERAGVRY*KWLLAVQR*QYGGLDQ*RWKSELPAGLSDVHFFSSQE 435
Query: 313 RSKRAGLLRVDAAGQLRVGVRLQVEVRAVPRXLRHPGEDAPDVCEVVPGLPHG 155
RSKRA LLRV+ GQLRVG L EVRAV R + GED+ DV EVVP L HG
Sbjct: 434 RSKRAWLLRVEPHGQLRVGFWLHREVRAVLRGF*NAGEDSKDVGEVVPRLSHG 276
Score = 31.3 bits (62), Expect(2) = 2e-060
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = -2 / -1
Query: 591 SYEGNAFVSATGERD 547
SYEGNA V A GERD
Sbjct: 709 SYEGNALVQAVGERD 665
>gb|CA018105.1|CA018105 HV07H15r HV Hordeum vulgare subsp. vulgare cDNA clone HV07H15
5-PRIME, mRNA sequence
Length = 504
Score = 216 bits (466), Expect(2) = 1e-057
Identities = 80/130 (61%), Positives = 106/130 (81%)
Frame = -2 / +1
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
DGV IG TA G++DVPE +EL ++YVN RY+ PVY+TENG+ Q+SD S ++LI+DV
Sbjct: 43 DGVAIGRPTAFPGYYDVPEGMELIVKYVNQRYENAPVYVTENGFSQFSDNSMKDLINDVG 222
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRM 187
R NYLQGY+T +S A+R GANVRGYFVW+L+DNFEW FG+ +++GLY+VDF+TQERT +M
Sbjct: 223 RVNYLQGYLTCISSAVRRGANVRGYFVWSLMDNFEWGFGFTVRFGLYYVDFETQERTPKM 402
Query: 186 SARWYQGFLT 157
S +WY+ LT
Sbjct: 403 SGKWYRDXLT 432
Score = 122 bits (262), Expect = 2e-027
Identities = 62/117 (52%), Positives = 68/117 (58%)
Frame = +2 / -2
Query: 173 YHLADIRXVLSWVSKSTWYSPYFNL*PNAHSKLSSSVHTK*PRTFAPFLSALDRYVM*PC 352
YH DI VLS VSKST YSP + P HSKLS +HTK*PRTFAP L+A + +V *PC
Sbjct: 416 YHFPDIFGVLSCVSKST*YSPNLTVKPKPHSKLSMRLHTK*PRTFAPLLTAEEMHVR*PC 237
Query: 353 R*FFXXXXXXXXXXXXXXHCX*PFSVIYTGVPLYLPFTYWIASSMASGTSKKPARAV 523
R*F +C PFSV YTG YL TY SSM SGTS P AV
Sbjct: 236 R*FTLPTSLIKSFILLSLNCEKPFSVTYTGAFSYLWLTYLTISSMPSGTS**PGNAV 66
Score = 107 bits (228), Expect = 9e-023
Identities = 59/113 (52%), Positives = 72/113 (63%)
Frame = -1 / +3
Query: 493 RGHRAGYPVREREVQGHACVYY*ERLLAVE*CQQGGADRRCEEEELPAGLHHVSIQSTQE 314
R H A V + E++ A V Y*+ LLAV+ Q G D+ + ELPAGL + + S+QE
Sbjct: 96 RRHGADRQVCQPEIRERAGVRY*KWLLAVQR*QYEGLDQ*RWKSELPAGLSDMHLFSSQE 275
Query: 313 RSKRAGLLRVDAAGQLRVGVRLQVEVRAVPRXLRHPGEDAPDVCEVVPGLPHG 155
RSKRA LLRV+ GQLRVG L EVRAV R R+ GED+ DV EVVP HG
Sbjct: 276 RSKRAWLLRVEPHGQLRVGFWLHREVRAVLRGFRNAGEDSKDVGEVVPRXXHG 434
Score = 27.2 bits (53), Expect(2) = 1e-057
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = -2 / +1
Query: 588 YEGNAFVSATGERD 547
+EGNA V A GERD
Sbjct: 4 HEGNALVQAVGERD 45
>gb|BF623927.2|BF623927 HVSMEa0007M14f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0007M14f, mRNA sequence
Length = 725
Score = 124 bits (266), Expect(3) = 1e-056
Identities = 45/90 (50%), Positives = 65/90 (72%)
Frame = -2 / +1
Query: 405 SDASREELIDDVRRKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLY 226
S + ++ D R NY+ GY+TYL+ A+R GA+VRGYFVW+LLD+FEW GY+ ++ LY
Sbjct: 334 SSMTAKDFTGDTERINYISGYLTYLASAIRKGADVRGYFVWSLLDDFEWTSGYKDRFXLY 513
Query: 225 HVDFDTQERTXRMSARWYQGFLTAXTSQRD 136
HVDF TQ+RT ++SA W++ FL + R+
Sbjct: 514 HVDFKTQKRTPKLSAEWFRQFLKSSLLTRE 603
Score = 73.0 bits (153), Expect(3) = 1e-056
Identities = 29/63 (46%), Positives = 41/63 (65%)
Frame = -2 / +1
Query: 735 REILGSDLPTFTAEEKALLLRYKADFIGLNHYTAIYARDCLRSPCNLGSYEGNAFVSATG 556
++ILG LP FT+++K L K DFIGLNHY+ Y +DC+ SPC + +G+A +
Sbjct: 1 QKILGPTLPEFTSKQKKKLHATKLDFIGLNHYSTWYLKDCIFSPCEMDPMDGDARALSLA 180
Query: 555 ERD 547
ERD
Sbjct: 181 ERD 189
Score = 61.1 bits (127), Expect(3) = 1e-056
Identities = 25/51 (49%), Positives = 30/51 (58%)
Frame = -2 / +1
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDAS 394
DGV IG T F+DVP +E + Y RY T YITENGY Q S++S
Sbjct: 187 DGVLIGKQTGAPFFYDVPHGMEKVVMYYKQRYNNTATYITENGYAQASNSS 339
>gb|BJ475687.1|BJ475687 BJ475687 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal29i07 3', mRNA sequence
Length = 645
Score = 215 bits (464), Expect = 2e-055
Identities = 80/130 (61%), Positives = 105/130 (80%)
Frame = -2 / -1
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
DGV I TA G++DVPE +EL ++YVN RY+ PVY+TENG+ Q+SD S ++LI+DV
Sbjct: 642 DGVAIXRPTAFPGYYDVPEGMELIVKYVNQRYENAPVYVTENGFSQFSDNSMQDLINDVG 463
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRM 187
R NYLQGY T +S A+R GANVRGYFVW+L+DNFEW FG+ +++GLY+VDF+TQERT +M
Sbjct: 462 RVNYLQGYXTCISSAVRRGANVRGYFVWSLMDNFEWGFGFTVRFGLYYVDFETQERTPKM 283
Query: 186 SARWYQGFLT 157
S +WY+ FLT
Sbjct: 282 SGKWYRDFLT 253
Score = 123 bits (263), Expect = 1e-027
Identities = 63/122 (51%), Positives = 70/122 (57%)
Frame = +2 / +2
Query: 158 VRKPWYHLADIRXVLSWVSKSTWYSPYFNL*PNAHSKLSSSVHTK*PRTFAPFLSALDRY 337
VRK YH DI VLS VSKST Y P + P HSKLS +HTK*PRTFAP L+A + +
Sbjct: 254 VRKSRYHFPDIFGVLSCVSKST*YXPNLTVKPKPHSKLSMRLHTK*PRTFAPLLTAEEMH 433
Query: 338 VM*PCR*FFXXXXXXXXXXXXXXHCX*PFSVIYTGVPLYLPFTYWIASSMASGTSKKPAR 517
V *PCR*F +C PFSV YTG YL TY SS+ SGTS P
Sbjct: 434 VX*PCR*FTLPTSLIKSCILLSLNCEKPFSVTYTGAFSYLWLTYLTISSIPSGTS**PGN 613
Query: 518 AV 523
AV
Sbjct: 614 AV 619
Score = 103 bits (219), Expect = 2e-021
Identities = 58/113 (51%), Positives = 71/113 (62%)
Frame = -1 / -3
Query: 493 RGHRAGYPVREREVQGHACVYY*ERLLAVE*CQQGGADRRCEEEELPAGLHHVSIQSTQE 314
R + A V + E++ A V Y*+ LLAV+ Q G D+ + ELPAGL +V S+QE
Sbjct: 589 RRNGADRQVCQPEIRERAGVRY*KWLLAVQR*QYAGLDQ*RWKSELPAGLSNVHFFSSQE 410
Query: 313 RSKRAGLLRVDAAGQLRVGVRLQVEVRAVPRXLRHPGEDAPDVCEVVPGLPHG 155
RSKRA LLRV+ GQLRVG L EVR V R + GED+ DV EVVP L HG
Sbjct: 409 RSKRAWLLRVEPHGQLRVGFWLHREVRXVLRGF*NAGEDSKDVGEVVPRLSHG 251
>gb|BJ467881.1|BJ467881 BJ467881 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30c23 3', mRNA sequence
Length = 670
Score = 210 bits (452), Expect = 1e-053
Identities = 77/125 (61%), Positives = 102/125 (81%)
Frame = -2 / -1
Query: 531 GGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVRRKNYL 352
G TA G++DVPE + L ++YVN RY+ PVY+TENG+ Q+SD S E+LI+DV R NYL
Sbjct: 670 GRPTAFPGYYDVPEGMXLIVKYVNQRYENAPVYVTENGFSQFSDNSMEDLINDVGRVNYL 491
Query: 351 QGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRMSARWY 172
QGY+T +S A+R GANVRGYFVW+L+DNFEW FG+ +++GLY+VDF+TQERT +MS +WY
Sbjct: 490 QGYLTCISSAVRRGANVRGYFVWSLMDNFEWGFGFTVRFGLYYVDFETQERTPKMSGKWY 311
Query: 171 QGFLT 157
+ FLT
Sbjct: 310 RDFLT 296
Score = 124 bits (265), Expect = 7e-028
Identities = 63/122 (51%), Positives = 70/122 (57%)
Frame = +2 / +3
Query: 158 VRKPWYHLADIRXVLSWVSKSTWYSPYFNL*PNAHSKLSSSVHTK*PRTFAPFLSALDRY 337
VRK YH DI VLS VSKST YSP + P HSKLS +HTK*PRTFAP L+A + +
Sbjct: 297 VRKSRYHFPDIFGVLSCVSKST*YSPNLTVKPKPHSKLSMRLHTK*PRTFAPLLTAEEMH 476
Query: 338 VM*PCR*FFXXXXXXXXXXXXXXHCX*PFSVIYTGVPLYLPFTYWIASSMASGTSKKPAR 517
V *PCR*F +C PFSV YTG YL TY S + SGTS P
Sbjct: 477 VR*PCR*FTLPTSLIKSSILLSLNCEKPFSVTYTGAFSYLWLTYLTISXIPSGTS**PGN 656
Query: 518 AV 523
AV
Sbjct: 657 AV 662
Score = 107 bits (228), Expect = 9e-023
Identities = 60/113 (53%), Positives = 72/113 (63%)
Frame = -1 / -3
Query: 493 RGHRAGYPVREREVQGHACVYY*ERLLAVE*CQQGGADRRCEEEELPAGLHHVSIQSTQE 314
R + A V + E++ A V Y*+ LLAV+ Q GG D+ + ELPAGL V S+QE
Sbjct: 632 RRNXADRQVCQPEIRERAGVRY*KWLLAVQR*QYGGLDQ*RWKSELPAGLSDVHFFSSQE 453
Query: 313 RSKRAGLLRVDAAGQLRVGVRLQVEVRAVPRXLRHPGEDAPDVCEVVPGLPHG 155
RSKRA LLRV+ GQLRVG L EVRAV R + GED+ DV EVVP L HG
Sbjct: 452 RSKRAWLLRVEPHGQLRVGFWLHREVRAVLRGF*NAGEDSKDVGEVVPRLSHG 294
>gb|CB881964.1|CB881964 HM11G24w HM Hordeum vulgare subsp. vulgare cDNA clone HM11G24
3-PRIME, mRNA sequence
Length = 653
Score = 200 bits (431), Expect = 9e-051
Identities = 73/115 (63%), Positives = 97/115 (84%)
Frame = -2 / -3
Query: 501 DVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVRRKNYLQGYITYLSKA 322
DVPE +EL ++YVN RY+ PVY+TENG+ Q+SD S ++LI+DV R NYLQGY+T +S A
Sbjct: 651 DVPEGMELIVKYVNQRYENAPVYVTENGFSQFSDNSMKDLINDVGRVNYLQGYLTCISSA 472
Query: 321 LRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRMSARWYQGFLT 157
+R GANVRGYFVW+L+DNFEW FG+ +++GLY+VDF+TQERT +MS +WY+ FLT
Sbjct: 471 VRRGANVRGYFVWSLMDNFEWGFGFTVRFGLYYVDFETQERTPKMSGKWYRDFLT 307
Score = 124 bits (265), Expect = 7e-028
Identities = 62/115 (53%), Positives = 68/115 (59%)
Frame = +2 / +2
Query: 158 VRKPWYHLADIRXVLSWVSKSTWYSPYFNL*PNAHSKLSSSVHTK*PRTFAPFLSALDRY 337
VRK YH DI VLS VSKST YSP + P HSKLS +HTK*PRTFAP L+A + +
Sbjct: 308 VRKSRYHFPDIFGVLSCVSKST*YSPNLTVKPKPHSKLSMRLHTK*PRTFAPLLTAEEMH 487
Query: 338 VM*PCR*FFXXXXXXXXXXXXXXHCX*PFSVIYTGVPLYLPFTYWIASSMASGTS 502
V *PCR*F +C PFSV YTG YL TY SSM SGTS
Sbjct: 488 VR*PCR*FTLPTSLIKSFILLSLNCEKPFSVTYTGAFSYLWLTYLTISSMPSGTS 652
Score = 110 bits (234), Expect = 1e-023
Identities = 60/113 (53%), Positives = 73/113 (64%)
Frame = -1 / -2
Query: 493 RGHRAGYPVREREVQGHACVYY*ERLLAVE*CQQGGADRRCEEEELPAGLHHVSIQSTQE 314
R H A V + E++ A V Y*+ LLAV+ Q G D+ + ELPAGL + + S+QE
Sbjct: 643 RRHGADRQVCQPEIRERAGVRY*KWLLAVQR*QYEGLDQ*RWKSELPAGLSDMHLFSSQE 464
Query: 313 RSKRAGLLRVDAAGQLRVGVRLQVEVRAVPRXLRHPGEDAPDVCEVVPGLPHG 155
RSKRA LLRV+ GQLRVG L EVRAV R R+ GED+ DV EVVP L HG
Sbjct: 463 RSKRAWLLRVEPHGQLRVGFWLHREVRAVLRGFRNAGEDSKDVGEVVPRLSHG 305
>gb|BE437689.1|BE437689 SFR006.D12F990705 ITEC SFR Barley Leaf Epidermis Library Hordeum
vulgare subsp. vulgare cDNA clone SF, mRNA sequence
Length = 531
Score = 84.0 bits (177), Expect(3) = 3e-047
Identities = 30/59 (50%), Positives = 43/59 (72%)
Frame = -2 / +3
Query: 405 SDASREELIDDVRRKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGL 229
S + ++ D R NY+ GY+TYL+ A+R GA+VR YFVW+LLD+FEW GY+ ++GL
Sbjct: 354 SSMTAKDFTGDTERINYISGYLTYLASAIRKGADVRCYFVWSLLDDFEWTSGYKDRFGL 530
Score = 82.2 bits (173), Expect(3) = 3e-047
Identities = 33/69 (47%), Positives = 44/69 (63%)
Frame = -2 / +3
Query: 753 DYPGAMREILGSDLPTFTAEEKALLLRYKADFIGLNHYTAIYARDCLRSPCNLGSYEGNA 574
DYP MR+ILG LP FT+++K L K DFIGLNHY+ Y +DC+ S C + +G+A
Sbjct: 3 DYPAEMRKILGPTLPEFTSKQKKKLHATKLDFIGLNHYSTWYLKDCIFSSCEMDPMDGDA 182
Query: 573 FVSATGERD 547
+ ERD
Sbjct: 183 RALSLAERD 209
Score = 61.1 bits (127), Expect(3) = 3e-047
Identities = 25/51 (49%), Positives = 30/51 (58%)
Frame = -2 / +3
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDAS 394
DGV IG T F+DVP +E + Y RY T YITENGY Q S++S
Sbjct: 207 DGVLIGKQTGAPFFYDVPHGMEKVVMYYKQRYNNTATYITENGYAQASNSS 359
>gb|BI958216.1|BI958216 HVSMEn0013O15f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0013O15f, mRNA sequence
Length = 648
Score = 92.3 bits (195), Expect(3) = 1e-045
Identities = 37/72 (51%), Positives = 48/72 (66%)
Frame = -2 / +3
Query: 762 ILGDYPGAMREILGSDLPTFTAEEKALLLRYKADFIGLNHYTAIYARDCLRSPCNLGSYE 583
ILGDYP MR+ILG LP FT+++K L K DFIGLNHY+ Y +DC+ SPC + +
Sbjct: 111 ILGDYPAEMRKILGPTLPEFTSKQKKKLHATKLDFIGLNHYSTWYLKDCIFSPCEMDPMD 290
Query: 582 GNAFVSATGERD 547
G+A + ERD
Sbjct: 291 GDARALSLAERD 326
Score = 71.6 bits (150), Expect(3) = 1e-045
Identities = 25/54 (46%), Positives = 36/54 (66%)
Frame = -2 / +3
Query: 405 SDASREELIDDVRRKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYR 244
S+ + ++ D R NY+ GY+TYL A+R G +RGYFVW LL++FEW G +
Sbjct: 471 SNMTAKDFTGDTERINYISGYLTYLPSAIRKGT*LRGYFVWALLNDFEWTLGIK 632
Score = 57.9 bits (120), Expect(3) = 1e-045
Identities = 23/51 (45%), Positives = 30/51 (58%)
Frame = -2 / +3
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDAS 394
DGV IG T F+DVP +E + Y RY T YIT+NGY Q S+++
Sbjct: 324 DGVLIGKQTGAPFFYDVPHGMEKVVMYYKQRYNNTATYITKNGYAQASNSN 476
>gb|BF629833.2|BF629833 HVSMEb0007A10f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0007A10f, mRNA sequence
Length = 493
Score = 126 bits (270), Expect(2) = 3e-045
Identities = 45/82 (54%), Positives = 63/82 (76%)
Frame = -2 / +2
Query: 405 SDASREELIDDVRRKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLY 226
S + ++ D R NY+ GY+TYL+ A+R GA+VRGYFVW+LLD+FEW GY+ ++GLY
Sbjct: 236 SSMTAKDFTGDTERINYISGYLTYLASAIRKGADVRGYFVWSLLDDFEWTSGYKDRFGLY 415
Query: 225 HVDFDTQERTXRMSARWYQGFL 160
HVDF TQ+RT ++SA W++ FL
Sbjct: 416 HVDFKTQKRTPKLSAEWFRQFL 481
Score = 77.1 bits (162), Expect(2) = 3e-045
Identities = 31/64 (48%), Positives = 42/64 (65%)
Frame = -2 / +3
Query: 738 MREILGSDLPTFTAEEKALLLRYKADFIGLNHYTAIYARDCLRSPCNLGSYEGNAFVSAT 559
MR+ILG LP FT+++K L K DFIGLNHY+ Y +DC+ SPC + +G+A +
Sbjct: 3 MRKILGPTLPEFTSKQKKKLHATKLDFIGLNHYSTWYLKDCIFSPCEMDPMDGDARALSL 182
Query: 558 GERD 547
ERD
Sbjct: 183 AERD 194
>gb|BQ134683.1|BQ134683 LP30159 Lemma/palea-enriched cDNA library from dough stage of
kernel Hordeum vulgare subsp. vulgare cDNA clone 3-159
similar to putative ABC transporter, mRNA sequence
Length = 339
Score = 159 bits (342), Expect = 2e-038
Identities = 57/88 (64%), Positives = 76/88 (86%)
Frame = -2 / +2
Query: 435 YITENGYXQWSDASREELIDDVRRKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWA 256
Y+TENG+ Q+SD S ++LI+DV R NYLQGY+T +S A+R GANVRGYFVW+L+DNFEW
Sbjct: 2 YVTENGFSQFSDNSMKDLINDVGRVNYLQGYLTCISSAVRRGANVRGYFVWSLMDNFEWG 181
Query: 255 FGYRLKYGLYHVDFDTQERTXRMSARWY 172
FG+ +++GLY+VDF+TQERT +MS +WY
Sbjct: 182 FGFTVRFGLYYVDFETQERTPKMSGKWY 265
>gb|CB858437.1|CB858437 HI07A02w HI Hordeum vulgare subsp. vulgare cDNA clone HI07A02
3-PRIME, mRNA sequence
Length = 653
Score = 123 bits (264), Expect(3) = 6e-035
Identities = 51/131 (38%), Positives = 76/131 (58%)
Frame = -2 / -1
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
+GV IG + VP + + Y+ +Y + I+ENG Q ++ +REE + D
Sbjct: 443 NGVPIGQKANSNWLYIVPTGMYGCVNYIREKYNNPTIIISENGMDQPANLTREEFLHDAS 264
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRM 187
R + + Y+ L KA+ +GANV GYF W+LLDNFEW GY K+G+ +VDF T +R +
Sbjct: 263 RVEFYKTYLAELKKAIDDGANVVGYFAWSLLDNFEWLSGYTSKFGIVYVDFTTLKRYPKD 84
Query: 186 SARWYQGFLTA 154
SA W++ L A
Sbjct: 83 SAYWFKNMLQA 51
Score = 31.3 bits (62), Expect(3) = 6e-035
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = -2 / -1
Query: 693 EKALLLRYKADFIGLNHYTAIYARD 619
E++ L++ D+IG+N YTA Y D
Sbjct: 587 EQSKLVKGSVDYIGINQYTATYMAD 513
Score = 30.4 bits (60), Expect(3) = 6e-035
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = -2 / -1
Query: 756 GDYPGAMREILGSDLPTFTAEEKALL 679
G YP M++I+ LP+FT E+ L+
Sbjct: 647 GQYPKTMQDIVKERLPSFTPEQSKLV 570
>gb|AL508271.1|AL508271 AL508271 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY08E11V 5',
mRNA sequence
Length = 700
Score = 86.3 bits (182), Expect(2) = 4e-033
Identities = 28/44 (63%), Positives = 39/44 (88%)
Frame = -2 / -1
Query: 288 VWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRMSARWYQGFLT 157
VW+L+DNFEW FG+ +++GLY+VDF+TQERT +MS +WY+ FLT
Sbjct: 496 VWSLMDNFEWGFGFTVRFGLYYVDFETQERTPKMSGKWYRDFLT 365
Score = 75.3 bits (158), Expect(2) = 4e-033
Identities = 30/56 (53%), Positives = 39/56 (69%)
Frame = -2 / -2
Query: 471 QYVNGRYKGTPVYITENGYXQWSDASREELIDDVRRKNYLQGYITYLSKALRNGAN 304
+YV + PVY+TENG+ Q+S E LI+DV R NYLQGY+T +S A+R GAN
Sbjct: 681 KYVQPEIRDAPVYVTENGFSQFSGXQYEGLINDVGRVNYLQGYLTCISSAVRRGAN 514
>gb|BJ462494.1|BJ462494 BJ462494 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags28l23 5', mRNA sequence
Length = 610
Score = 114 bits (244), Expect(3) = 3e-032
Identities = 48/122 (39%), Positives = 71/122 (58%)
Frame = -2 / +1
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
+GV IG + VP + + Y+ +Y + I+ENG Q ++ +REE + D
Sbjct: 244 NGVPIGQKANSNWLYIVPTGMYGCVNYIREKYNNPTIIISENGMDQPANLTREEFLHDAS 423
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRM 187
R + + Y+ L KA+ +GANV GYF W+LLDNFEW GY K+G+ +VDF T +R +
Sbjct: 424 RVEFYKTYLAELKKAIDDGANVVGYFAWSLLDNFEWLSGYTSKFGIVYVDFTTLKRYPKD 603
Query: 186 SA 181
SA
Sbjct: 604 SA 609
Score = 31.3 bits (62), Expect(3) = 3e-032
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = -2 / +1
Query: 693 EKALLLRYKADFIGLNHYTAIYARD 619
E++ L++ D+IG+N YTA Y D
Sbjct: 100 EQSKLVKGSVDYIGINQYTATYMAD 174
Score = 30.4 bits (60), Expect(3) = 3e-032
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = -2 / +1
Query: 756 GDYPGAMREILGSDLPTFTAEEKALL 679
G YP M++I+ LP+FT E+ L+
Sbjct: 40 GQYPKTMQDIVKERLPSFTPEQSKLV 117
>gb|AV920504.1|AV920504 AV920504 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags14f05 3', mRNA sequence
Length = 569
Score = 95.0 bits (201), Expect(2) = 9e-032
Identities = 31/47 (65%), Positives = 42/47 (89%)
Frame = -2 / -1
Query: 297 GYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRMSARWYQGFLT 157
GYFVW+L+DNFEW FG+ +++GLY+VDF+TQERT +MS +WY+ FLT
Sbjct: 446 GYFVWSLMDNFEWGFGFTVRFGLYYVDFETQERTPKMSGKWYRDFLT 306
Score = 62.0 bits (129), Expect(2) = 9e-032
Identities = 25/40 (62%), Positives = 32/40 (80%)
Frame = -2 / -3
Query: 417 YXQWSDASREELIDDVRRKNYLQGYITYLSKALRNGANVR 298
+ Q+SD S E+LI+DV R NYLQGY+T +S A+R GANVR
Sbjct: 567 FSQFSDNSMEDLINDVXRVNYLQGYLTCISSAVRRGANVR 448
>gb|BJ467618.1|BJ467618 BJ467618 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags29b06 3', mRNA sequence
Length = 709
Score = 121 bits (258), Expect(2) = 7e-030
Identities = 52/130 (40%), Positives = 73/130 (56%)
Frame = -2 / -2
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
+GV IG + VP I A+ YV RY + + ENG Q + S + + D
Sbjct: 540 NGVPIGAHANSYWLYIVPWGINKAVTYVKERYGNPTMILAENGMDQPGNVSITDGVQDTV 361
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRM 187
R Y + YIT L KA+ +GA V GYF W+LLDNFEW GY ++G+ +VD+ T +R +
Sbjct: 360 RVRYYRDYITELKKAIDDGARVIGYFAWSLLDNFEWRLGYTARFGIVYVDYKTLKRYPKD 181
Query: 186 SARWYQGFLT 157
SA W++ LT
Sbjct: 180 SALWFKNMLT 151
Score = 29.5 bits (58), Expect(2) = 7e-030
Identities = 7/25 (28%), Positives = 18/25 (72%)
Frame = -2 / -2
Query: 693 EKALLLRYKADFIGLNHYTAIYARD 619
+++ +++ D++G+N YT+ Y +D
Sbjct: 684 DESTMIKGSIDYVGINQYTSFYMKD 610
>gb|BQ755347.1|BQ755347 EBed07_SQ002_J07_R endosperm, 28 DPA, no treatment, cv Optic,
EBed07 Hordeum vulgare subsp. vulgare cDNA clone
EBed07_SQ002_J07 5', mRNA sequence
Length = 642
Score = 121 bits (259), Expect(2) = 1e-029
Identities = 52/130 (40%), Positives = 75/130 (57%)
Frame = -2 / +3
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
+GV IG + VP + A+ YV RY + ++ENG Q + S + + D
Sbjct: 159 NGVPIGPRANSDWLYIVPWGMNKAVTYVKERYGNPTMILSENGMDQPGNVSIADGVHDTV 338
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRM 187
R Y + YIT L KA+ NGA V GYF W+LLDNFEW GY ++G+ +VDF+T +R +
Sbjct: 339 RIRYYRDYITELKKAIDNGARVAGYFAWSLLDNFEWRLGYTARFGIVYVDFNTLKRYPKD 518
Query: 186 SARWYQGFLT 157
SA W++ L+
Sbjct: 519 SALWFKNMLS 548
Score = 28.1 bits (55), Expect(2) = 1e-029
Identities = 7/25 (28%), Positives = 18/25 (72%)
Frame = -2 / +3
Query: 693 EKALLLRYKADFIGLNHYTAIYARD 619
+++ +++ D++G+N YT+ Y +D
Sbjct: 15 DESRMVKGSIDYVGINQYTSYYMKD 89
>gb|BU986792.1|BU986792 HF12O16r HF Hordeum vulgare subsp. vulgare cDNA clone HF12O16
5-PRIME, mRNA sequence
Length = 615
Score = 121 bits (259), Expect(2) = 1e-029
Identities = 52/130 (40%), Positives = 75/130 (57%)
Frame = -2 / +1
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
+GV IG + VP + A+ YV RY + ++ENG Q + S + + D
Sbjct: 169 NGVPIGPRANSDWLYIVPWGMNKAVTYVKERYGNPTMILSENGMDQPGNVSIADGVHDTV 348
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRM 187
R Y + YIT L KA+ NGA V GYF W+LLDNFEW GY ++G+ +VDF+T +R +
Sbjct: 349 RIRYYRDYITELKKAIDNGARVAGYFAWSLLDNFEWRLGYTARFGIVYVDFNTLKRYPKD 528
Query: 186 SARWYQGFLT 157
SA W++ L+
Sbjct: 529 SALWFKNMLS 558
Score = 28.1 bits (55), Expect(2) = 1e-029
Identities = 7/25 (28%), Positives = 18/25 (72%)
Frame = -2 / +1
Query: 693 EKALLLRYKADFIGLNHYTAIYARD 619
+++ +++ D++G+N YT+ Y +D
Sbjct: 25 DESRMVKGSIDYVGINQYTSYYMKD 99
>gb|BU984559.1|BU984559 HF04E16r HF Hordeum vulgare subsp. vulgare cDNA clone HF04E16
5-PRIME, mRNA sequence
Length = 590
Score = 121 bits (259), Expect(2) = 3e-029
Identities = 52/130 (40%), Positives = 75/130 (57%)
Frame = -2 / +2
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
+GV IG + VP + A+ YV RY + ++ENG Q + S + + D
Sbjct: 125 NGVPIGPRANSDWLYIVPWGMNKAVTYVKERYGNPTMILSENGMDQPGNVSIADGVHDTV 304
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRM 187
R Y + YIT L KA+ NGA V GYF W+LLDNFEW GY ++G+ +VDF+T +R +
Sbjct: 305 RIRYYRDYITELKKAIDNGARVAGYFAWSLLDNFEWRLGYTARFGIVYVDFNTLKRYPKD 484
Query: 186 SARWYQGFLT 157
SA W++ L+
Sbjct: 485 SALWFKNMLS 514
Score = 26.7 bits (52), Expect(2) = 3e-029
Identities = 7/15 (46%), Positives = 12/15 (80%)
Frame = -2 / +2
Query: 663 DFIGLNHYTAIYARD 619
D++G+N YT+ Y +D
Sbjct: 11 DYVGINQYTSYYMKD 55
>gb|AV832993.1|AV832993 AV832993 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare top three leaves adult, heading stage Hordeum
vulgare subsp. vulgare cDNA clone baal5f06, mRNA
sequence
Length = 694
Score = 83.1 bits (175), Expect(4) = 6e-029
Identities = 33/67 (49%), Positives = 43/67 (64%)
Frame = -2 / -2
Query: 399 ASREELIDDVRRKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHV 220
A ++++D R YL+ YI A RNGANV+GYF W LD FE+ GY+L YG+Y V
Sbjct: 453 AYANDILNDTDRVEYLKTYIGSTLDASRNGANVKGYFAWNFLDIFEFLAGYKLGYGMYRV 274
Query: 219 DFDTQER 199
DFD + R
Sbjct: 273 DFDDEAR 253
Score = 37.7 bits (76), Expect(4) = 6e-029
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -2 / -2
Query: 501 DVPEAIELAIQYVNGRYKGTPVYITENGYXQWSD 400
D PE + +QY+ Y G P+Y+ ENG +D
Sbjct: 540 DDPEGLRFVLQYLTEAYGGLPIYVQENGKAYAND 439
Score = 31.3 bits (62), Expect(4) = 6e-029
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = -2 / -2
Query: 219 DFDTQERTXRMSARWYQGFL 160
D + + R R+SARWY GFL
Sbjct: 267 DDEARPREARLSARWYAGFL 208
Score = 30.4 bits (60), Expect(4) = 6e-029
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = -2 / -2
Query: 663 DFIGLNHYTAIYARD 619
DFIG+NHY ++Y D
Sbjct: 693 DFIGINHYYSVYVND 649
>gb|BI947748.1|BI947748 HVSMEl0006L16f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0006L16f, mRNA sequence
Length = 833
Score = 127 bits (271), Expect = 1e-028
Identities = 54/130 (41%), Positives = 77/130 (59%)
Frame = -2 / -3
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
+GV IG F+ VP + A+ YV RY P+ ++ENG Q + S + + D
Sbjct: 738 NGVPIGLRANSDWFYIVPWGMNKAVTYVKERYGNPPMILSENGMDQPGNVSIADGVHDTV 559
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRM 187
R Y + YIT L KA+ NGA V GYF W+LLDNFEW GY ++G+ +VDF+T +R +
Sbjct: 558 RIRYYRDYITELKKAIDNGARVAGYFAWSLLDNFEWRLGYTARFGIVYVDFNTLKRYPKD 379
Query: 186 SARWYQGFLT 157
SA W++ L+
Sbjct: 378 SALWFKNMLS 349
>gb|BI954140.1|BI954140 HVSMEm0016F19f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0016F19f, mRNA sequence
Length = 683
Score = 78.5 bits (165), Expect(5) = 2e-028
Identities = 33/73 (45%), Positives = 44/73 (60%)
Frame = -2 / +1
Query: 417 YXQWSDASREELIDDVRRKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLK 238
Y Q + + + + D R YL+ Y+ KA+RNGANV+GYFVW LD FE+ G +
Sbjct: 421 YVQENGVASNDTLYDTERIEYLKSYMGGTLKAVRNGANVKGYFVWCFLDVFEFFAGAPSR 600
Query: 237 YGLYHVDFDTQER 199
YGLY VDF+ R
Sbjct: 601 YGLYRVDFNDDAR 639
Score = 33.6 bits (67), Expect(5) = 2e-028
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -2 / +1
Query: 495 PEAIELAIQYVNGRYKGTPVYITENG 418
PE ++ +QY+ Y P+Y+ ENG
Sbjct: 361 PEGLQYVLQYLTKAYGNIPIYVQENG 438
Score = 31.8 bits (63), Expect(5) = 2e-028
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = -2 / +3
Query: 762 ILGDYPGAMREILGSDLPTFT 700
+ GDYP M++ +GS LP+FT
Sbjct: 105 VSGDYPDVMKKNVGSRLPSFT 167
Score = 29.5 bits (58), Expect(5) = 2e-028
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = -2 / +1
Query: 663 DFIGLNHYTAIYARD 619
DFIG+NHY ++Y D
Sbjct: 202 DFIGINHYYSMYVND 246
Score = 24.0 bits (46), Expect(5) = 2e-028
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = -2 / +3
Query: 198 TXRMSARWYQGFL 160
T ++SARW GFL
Sbjct: 645 TAKLSARWDSGFL 683
>gb|BJ463795.1|BJ463795 BJ463795 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30i11 5', mRNA sequence
Length = 669
Score = 110 bits (235), Expect(3) = 3e-028
Identities = 44/100 (44%), Positives = 66/100 (66%)
Frame = -2 / +3
Query: 495 PEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVRRKNYLQGYITYLSKALR 316
P + ++++ +YK V I ENG +D S ++ +DD R YLQ YI ++ R
Sbjct: 174 PWVLREMLEHLQVKYKNPVVMIHENGAASVADPSGDKALDDEFRSRYLQDYIEATLQSSR 353
Query: 315 NGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERT 196
NG+NV+GYFVW+ +D FE+ FGYR+ +GLY VDF+++ERT
Sbjct: 354 NGSNVQGYFVWSFVDMFEFLFGYRMGFGLYGVDFNSEERT 473
Score = 27.2 bits (53), Expect(3) = 3e-028
Identities = 8/14 (57%), Positives = 10/14 (71%)
Frame = -2 / +3
Query: 663 DFIGLNHYTAIYAR 622
DF+G NHY A Y +
Sbjct: 30 DFVGFNHYAASYVK 71
Score = 25.4 bits (49), Expect(3) = 3e-028
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = -2 / +3
Query: 213 DTQERTXRMSARWYQGFL 160
+ + R R SA+WY GFL
Sbjct: 462 EERTRYRRHSAKWYAGFL 515
>gb|BG301241.1|BG301241 HVSMEb0020C10f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0020C10f, mRNA sequence
Length = 895
Score = 101 bits (216), Expect(3) = 1e-027
Identities = 44/100 (44%), Positives = 61/100 (61%)
Frame = -2 / +3
Query: 495 PEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVRRKNYLQGYITYLSKALR 316
P A+ + ++ +YK V I ENG +D S DD YLQ YI ++ R
Sbjct: 390 PWALNKMLGHLQLKYKNPVVMIHENGAASIADPSAGNAPDDEFMSQYLQDYIEATLESSR 569
Query: 315 NGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERT 196
NG+NVRGYFVW+ LD FE+ FGYR+ + LY VDF+++E+T
Sbjct: 570 NGSNVRGYFVWSFLDVFEYLFGYRMGFELYGVDFNSEEKT 689
Score = 34.1 bits (68), Expect(3) = 1e-027
Identities = 14/27 (51%), Positives = 18/27 (66%)
Frame = -2 / +3
Query: 756 GDYPGAMREILGSDLPTFTAEEKALLL 676
GDYP MR+ +G LP+FT EE +L
Sbjct: 126 GDYPPVMRKNVGCRLPSFTDEELKTVL 206
Score = 29.0 bits (57), Expect(3) = 1e-027
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = -2 / +3
Query: 663 DFIGLNHYTAIYAR 622
DF+G NHY A+Y +
Sbjct: 216 DFVGFNHYIAVYVK 257
>gb|BE601818.2|BE601818 HVSMEh0099K11f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0099K11f, mRNA sequence
Length = 503
Score = 122 bits (261), Expect = 2e-027
Identities = 53/137 (38%), Positives = 77/137 (56%)
Frame = -2 / +1
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
+GV IG + VP + A+ YV RY + ++ENG Q + S + + D
Sbjct: 67 NGVPIGPRANSDWLYIVPWGMNKAVTYVKERYGNPTMILSENGMDQPGNVSIADGVHDTV 246
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRM 187
R Y + YIT L KA+ NGA V GYF W+LLDNFEW GY ++G+ +VDF+T +R +
Sbjct: 247 RIRYYRDYITELKKAIDNGARVAGYFAWSLLDNFEWRLGYTARFGIVYVDFNTLKRYPKD 426
Query: 186 SARWYQGFLTAXTSQRD 136
SA W++ L+ + D
Sbjct: 427 SALWFKNMLSEKKRELD 477
>gb|BI951308.1|BI951308 HVSMEl0025H09f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0025H09f, mRNA sequence
Length = 565
Score = 121 bits (259), Expect = 5e-027
Identities = 52/130 (40%), Positives = 75/130 (57%)
Frame = -2 / +1
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
+GV IG + VP + A+ YV RY + ++ENG Q + S + + D
Sbjct: 52 NGVPIGPRANSDWLYIVPWGMNKAVTYVKERYGNPTMILSENGMDQPGNVSIADGVHDTV 231
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRM 187
R Y + YIT L KA+ NGA V GYF W+LLDNFEW GY ++G+ +VDF+T +R +
Sbjct: 232 RIRYYRDYITELKKAIDNGARVAGYFAWSLLDNFEWRLGYTARFGIVYVDFNTLKRYPKD 411
Query: 186 SARWYQGFLT 157
SA W++ L+
Sbjct: 412 SALWFKNMLS 441
>gb|BQ466282.1|BQ466282 HT02B16r HT Hordeum vulgare subsp. vulgare cDNA clone HT02B16
5-PRIME, mRNA sequence
Length = 611
Score = 121 bits (259), Expect = 5e-027
Identities = 52/130 (40%), Positives = 75/130 (57%)
Frame = -2 / +1
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
+GV IG + VP + A+ YV RY + ++ENG Q + S + + D
Sbjct: 43 NGVPIGPRANSDWLYIVPWGMNKAVTYVKERYGNPTMILSENGMDQPGNVSIADGVHDTV 222
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRM 187
R Y + YIT L KA+ NGA V GYF W+LLDNFEW GY ++G+ +VDF+T +R +
Sbjct: 223 RIRYYRDYITELKKAIDNGARVAGYFAWSLLDNFEWRLGYTARFGIVYVDFNTLKRYPKD 402
Query: 186 SARWYQGFLT 157
SA W++ L+
Sbjct: 403 SALWFKNMLS 432
>gb|AV929747.1|AV929747 AV929747 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd16g11 3', mRNA sequence
Length = 644
Score = 83.1 bits (175), Expect(4) = 8e-027
Identities = 34/73 (46%), Positives = 47/73 (64%)
Frame = -2 / -3
Query: 417 YXQWSDASREELIDDVRRKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLK 238
Y Q + + + + D R YL+ Y+ KA+RNGANV+GYFVW+ LD FE+ G R +
Sbjct: 423 YVQENGVASNDTLYDTERIEYLKSYMGGTLKAVRNGANVKGYFVWSFLDVFEFFAGPRSR 244
Query: 237 YGLYHVDFDTQER 199
YGLY VDF+ + R
Sbjct: 243 YGLYRVDFNDEAR 205
Score = 33.6 bits (67), Expect(4) = 8e-027
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -2 / -3
Query: 495 PEAIELAIQYVNGRYKGTPVYITENG 418
PE ++ +QY+ Y P+Y+ ENG
Sbjct: 483 PEGLQYVLQYLTKAYGNIPIYVQENG 406
Score = 29.5 bits (58), Expect(4) = 8e-027
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = -2 / -3
Query: 663 DFIGLNHYTAIYARD 619
DFIG+NHY ++Y D
Sbjct: 642 DFIGINHYYSMYVND 598
Score = 29.0 bits (57), Expect(4) = 8e-027
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = -2 / -3
Query: 213 DTQERTXRMSARWYQGFL 160
+ + R ++SARWY GFL
Sbjct: 213 EARPRQAKLSARWYSGFL 160
>gb|BE601804.3|BE601804 HVSMEh0099J17f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0099J17f, mRNA sequence
Length = 627
Score = 117 bits (251), Expect = 6e-026
Identities = 48/114 (42%), Positives = 69/114 (60%)
Frame = -2 / +2
Query: 498 VPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVRRKNYLQGYITYLSKAL 319
VP + A+ YV RY + ++ENG Q + S + + D R Y + YIT L KA+
Sbjct: 23 VPWGMNKAVTYVKERYGNPTMILSENGMDQPGNVSIADGVHDTVRIRYYRDYITELKKAI 202
Query: 318 RNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRMSARWYQGFLT 157
NGA V GYF W+LLDNFEW GY ++G+ +VDF+T +R + SA W++ L+
Sbjct: 203 DNGARVAGYFAWSLLDNFEWRLGYTARFGIVYVDFNTLKRYPKDSALWFKNMLS 364
>gb|BQ659818.1|BQ659818 HF01M24w HF Hordeum vulgare subsp. vulgare cDNA clone HF01M24
3-PRIME, mRNA sequence
Length = 681
Score = 117 bits (251), Expect = 6e-026
Identities = 48/114 (42%), Positives = 69/114 (60%)
Frame = -2 / -1
Query: 498 VPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVRRKNYLQGYITYLSKAL 319
VP + A+ YV RY + ++ENG Q + S + + D R Y + YIT L KA+
Sbjct: 648 VPWGMNKAVTYVKERYGNPTMILSENGMDQPGNVSIADGVHDTVRIRYYRDYITELKKAI 469
Query: 318 RNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERTXRMSARWYQGFLT 157
NGA V GYF W+LLDNFEW GY ++G+ +VDF+T +R + SA W++ L+
Sbjct: 468 DNGARVAGYFAWSLLDNFEWRLGYTARFGIVYVDFNTLKRYPKDSALWFKNMLS 307
>gb|BU977487.1|BU977487 HA11I05r HA Hordeum vulgare subsp. vulgare cDNA clone HA11I05
5-PRIME, mRNA sequence
Length = 655
Score = 76.7 bits (161), Expect(4) = 1e-025
Identities = 28/77 (36%), Positives = 48/77 (62%)
Frame = -2 / +1
Query: 390 EELIDDVRRKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFD 211
+E + D R NY ++ +L+ A++ N++GYF WT +D FEW GY+ ++GL ++D
Sbjct: 394 KEALRDPARINYHYKHLLFLNLAIKQKVNIKGYFTWTFMDCFEWGDGYKDRFGLIYIDRS 573
Query: 210 TQERTXRMSARWYQGFL 160
T +R + S++W FL
Sbjct: 574 TLKRYPKDSSKWRGRFL 624
Score = 32.7 bits (65), Expect(4) = 1e-025
Identities = 15/51 (29%), Positives = 22/51 (43%)
Frame = -2 / +1
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDAS 394
DG +G F P I + Y RY VY+ ENG + +++S
Sbjct: 232 DGKPLGPQAYTEFLFVYPPGIHELMLYAKRRYNNPAVYVMENGIDEGNNSS 384
Score = 31.8 bits (63), Expect(4) = 1e-025
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = -2 / +1
Query: 756 GDYPGAMREILGSDLPTFTAEEKALL 679
GDYP +MR L + LP FT + A L
Sbjct: 25 GDYPASMRSWLRARLPAFTPAQTAAL 102
Score = 29.9 bits (59), Expect(4) = 1e-025
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = -2 / +1
Query: 678 LRYKADFIGLNHYTAIYA 625
LR DF+GLN+YT YA
Sbjct: 100 LRGSYDFVGLNYYTTYYA 153
>gb|BE422156.1|BE422156 HWM020cG.09r ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM020cG.09, mRNA sequence
Length = 1023
Score = 109 bits (232), Expect(2) = 2e-025
Identities = 43/113 (38%), Positives = 66/113 (58%)
Frame = -2 / +1
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
+G IG + VP + + Y++ +Y P+ ITENG Q +R++ + D
Sbjct: 370 NGKPIGPKANSNWLYIVPSGMYGCVHYLSQKYGNPPIVITENGMDQPGGLTRDQYLRDTT 549
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDT 208
R + + Y++ L KA+ GANV GYF W+LLDNFEW GY K+G+ +VDF++
Sbjct: 550 RVRFYRSYLSELKKAIDGGANVLGYFAWSLLDNFEWMSGYSXKFGIVYVDFNS 708
Score = 26.7 bits (52), Expect(2) = 2e-025
Identities = 9/19 (47%), Positives = 15/19 (78%)
Frame = -2 / +1
Query: 690 KALLLRYKADFIGLNHYTA 634
+A +++ AD+IG+N YTA
Sbjct: 229 EAKMVKGSADYIGINQYTA 285
>gb|BG365882.1|BG365882 HVSMEi0004J22f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0004J22f, mRNA sequence
Length = 923
Score = 110 bits (235), Expect(2) = 2e-025
Identities = 44/100 (44%), Positives = 66/100 (66%)
Frame = -2 / +2
Query: 495 PEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVRRKNYLQGYITYLSKALR 316
P + ++++ +YK V I ENG +D S ++ +DD R YLQ YI ++ R
Sbjct: 104 PWVLREMLEHLQVKYKNPVVMIHENGAASVADPSGDKALDDEFRSRYLQDYIEATLQSSR 283
Query: 315 NGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERT 196
NG+NV+GYFVW+ +D FE+ FGYR+ +GLY VDF+++ERT
Sbjct: 284 NGSNVQGYFVWSFVDMFEFLFGYRMGFGLYGVDFNSEERT 403
Score = 25.4 bits (49), Expect(2) = 2e-025
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = -2 / +2
Query: 213 DTQERTXRMSARWYQGFL 160
+ + R R SA+WY GFL
Sbjct: 392 EERTRYRRHSAKWYAGFL 445
>gb|AV918813.1|AV918813 AV918813 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags22f16 3', mRNA sequence
Length = 698
Score = 110 bits (235), Expect(2) = 2e-025
Identities = 44/100 (44%), Positives = 66/100 (66%)
Frame = -2 / -2
Query: 495 PEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVRRKNYLQGYITYLSKALR 316
P + ++++ +YK V I ENG +D S ++ +DD R YLQ YI ++ R
Sbjct: 601 PWVLREMLEHLQVKYKNPVVMIHENGAASVADPSGDKALDDEFRSRYLQDYIEATLQSSR 422
Query: 315 NGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERT 196
NG+NV+GYFVW+ +D FE+ FGYR+ +GLY VDF+++ERT
Sbjct: 421 NGSNVQGYFVWSFVDMFEFLFGYRMGFGLYGVDFNSEERT 302
Score = 25.4 bits (49), Expect(2) = 2e-025
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = -2 / -2
Query: 213 DTQERTXRMSARWYQGFL 160
+ + R R SA+WY GFL
Sbjct: 313 EERTRYRRHSAKWYAGFL 260
>gb|BJ467970.1|BJ467970 BJ467970 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30i11 3', mRNA sequence
Length = 691
Score = 110 bits (235), Expect(2) = 2e-025
Identities = 44/100 (44%), Positives = 66/100 (66%)
Frame = -2 / -2
Query: 495 PEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVRRKNYLQGYITYLSKALR 316
P + ++++ +YK V I ENG +D S ++ +DD R YLQ YI ++ R
Sbjct: 606 PWVLREMLEHLQVKYKNPVVMIHENGAASVADPSGDKALDDEFRSRYLQDYIEATLQSSR 427
Query: 315 NGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERT 196
NG+NV+GYFVW+ +D FE+ FGYR+ +GLY VDF+++ERT
Sbjct: 426 NGSNVQGYFVWSFVDMFEFLFGYRMGFGLYGVDFNSEERT 307
Score = 25.4 bits (49), Expect(2) = 2e-025
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = -2 / -2
Query: 213 DTQERTXRMSARWYQGFL 160
+ + R R SA+WY GFL
Sbjct: 318 EERTRYRRHSAKWYAGFL 265
>gb|BQ659752.1|BQ659752 HF01J17w HF Hordeum vulgare subsp. vulgare cDNA clone HF01J17
3-PRIME, mRNA sequence
Length = 681
Score = 110 bits (235), Expect(2) = 2e-025
Identities = 44/100 (44%), Positives = 66/100 (66%)
Frame = -2 / -3
Query: 495 PEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVRRKNYLQGYITYLSKALR 316
P + ++++ +YK V I ENG +D S ++ +DD R YLQ YI ++ R
Sbjct: 613 PWVLREMLEHLQVKYKNPVVMIHENGAASVADPSGDKALDDEFRSRYLQDYIEATLQSSR 434
Query: 315 NGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERT 196
NG+NV+GYFVW+ +D FE+ FGYR+ +GLY VDF+++ERT
Sbjct: 433 NGSNVQGYFVWSFVDMFEFLFGYRMGFGLYGVDFNSEERT 314
Score = 25.4 bits (49), Expect(2) = 2e-025
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = -2 / -3
Query: 213 DTQERTXRMSARWYQGFL 160
+ + R R SA+WY GFL
Sbjct: 325 EERTRYRRHSAKWYAGFL 272
>gb|AV921900.1|AV921900 AV921900 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19a14 3', mRNA sequence
Length = 662
Score = 110 bits (235), Expect(2) = 2e-025
Identities = 44/100 (44%), Positives = 66/100 (66%)
Frame = -2 / -2
Query: 495 PEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVRRKNYLQGYITYLSKALR 316
P + ++++ +YK V I ENG +D S ++ +DD R YLQ YI ++ R
Sbjct: 586 PWVLREMLEHLQVKYKNPVVMIHENGAASVADPSGDKALDDEFRSRYLQDYIEATLQSSR 407
Query: 315 NGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERT 196
NG+NV+GYFVW+ +D FE+ FGYR+ +GLY VDF+++ERT
Sbjct: 406 NGSNVQGYFVWSFVDMFEFLFGYRMGFGLYGVDFNSEERT 287
Score = 25.4 bits (49), Expect(2) = 2e-025
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = -2 / -2
Query: 213 DTQERTXRMSARWYQGFL 160
+ + R R SA+WY GFL
Sbjct: 298 EERTRYRRHSAKWYAGFL 245
>gb|BU972640.1|BU972640 HB22E11r BC Hordeum vulgare subsp. vulgare cDNA clone HB22E11
5-PRIME, mRNA sequence
Length = 591
Score = 108 bits (231), Expect(2) = 2e-025
Identities = 43/113 (38%), Positives = 66/113 (58%)
Frame = -2 / +1
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
+G IG + VP + + Y++ +Y P+ ITENG Q +R++ + D
Sbjct: 217 NGKPIGPKANSNWLYIVPSGMYGCVHYLSQKYGNPPIVITENGMDQPGGLTRDQYLRDTT 396
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDT 208
R + + Y++ L KA+ GANV GYF W+LLDNFEW GY K+G+ +VDF++
Sbjct: 397 RVRFYRSYLSELKKAIDGGANVLGYFAWSLLDNFEWMSGYSSKFGIVYVDFNS 555
Score = 26.7 bits (52), Expect(2) = 2e-025
Identities = 9/19 (47%), Positives = 15/19 (78%)
Frame = -2 / +1
Query: 690 KALLLRYKADFIGLNHYTA 634
+A +++ AD+IG+N YTA
Sbjct: 76 EAKMVKGSADYIGINQYTA 132
>gb|BQ664794.1|BQ664794 HV04O11u HV Hordeum vulgare subsp. vulgare cDNA clone HV04O11
3-PRIME, mRNA sequence
Length = 603
Score = 109 bits (233), Expect(2) = 3e-025
Identities = 43/93 (46%), Positives = 64/93 (68%)
Frame = -2 / -2
Query: 474 IQYVNGRYKGTPVYITENGYXQWSDASREELIDDVRRKNYLQGYITYLSKALRNGANVRG 295
++++ +YK V I ENG +D S ++ +DD R YLQ YI ++ RNG+NV+G
Sbjct: 596 LEHLQVKYKNPVVMIHENGAASVADPSGDKALDDEFRSRYLQDYIEATLQSSRNGSNVQG 417
Query: 294 YFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERT 196
YFVW+ +D FE+ FGYR+ +GLY VDF+++ERT
Sbjct: 416 YFVWSFVDMFEFLFGYRMGFGLYGVDFNSEERT 318
Score = 25.4 bits (49), Expect(2) = 3e-025
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = -2 / -2
Query: 213 DTQERTXRMSARWYQGFL 160
+ + R R SA+WY GFL
Sbjct: 329 EERTRYRRHSAKWYAGFL 276
>gb|BQ469152.1|BQ469152 HM03J01r HM Hordeum vulgare subsp. vulgare cDNA clone HM03J01
5-PRIME, mRNA sequence
Length = 578
Score = 108 bits (230), Expect(2) = 3e-025
Identities = 43/112 (38%), Positives = 65/112 (58%)
Frame = -2 / +3
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
+G IG + VP + + Y++ +Y P+ ITENG Q +R++ + D
Sbjct: 243 NGKPIGPKANSNWLYIVPSGMYGCVHYLSQKYGNPPIVITENGMDQPGGLTRDQYLRDTT 422
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFD 211
R + + Y++ L KA+ GANV GYF W+LLDNFEW GY K+G+ +VDF+
Sbjct: 423 RVRFYRSYLSELKKAIDGGANVLGYFAWSLLDNFEWMSGYSSKFGIVYVDFN 578
Score = 26.7 bits (52), Expect(2) = 3e-025
Identities = 9/19 (47%), Positives = 15/19 (78%)
Frame = -2 / +3
Query: 690 KALLLRYKADFIGLNHYTA 634
+A +++ AD+IG+N YTA
Sbjct: 102 EAKMVKGSADYIGINQYTA 158
>gb|BJ468579.1|BJ468579 BJ468579 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags39g17 3', mRNA sequence
Length = 661
Score = 110 bits (235), Expect(2) = 4e-025
Identities = 44/100 (44%), Positives = 66/100 (66%)
Frame = -2 / -1
Query: 495 PEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVRRKNYLQGYITYLSKALR 316
P + ++++ +YK V I ENG +D S ++ +DD R YLQ YI ++ R
Sbjct: 586 PWVLREMLEHLQVKYKNPVVMIHENGAASVADPSGDKALDDEFRSRYLQDYIEATLQSSR 407
Query: 315 NGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDTQERT 196
NG+NV+GYFVW+ +D FE+ FGYR+ +GLY VDF+++ERT
Sbjct: 406 NGSNVQGYFVWSFVDMFEFLFGYRMGFGLYGVDFNSEERT 287
Score = 24.0 bits (46), Expect(2) = 4e-025
Identities = 8/11 (72%), Positives = 9/11 (81%)
Frame = -2 / -2
Query: 192 RMSARWYQGFL 160
R SA+WY GFL
Sbjct: 276 RHSAKWYAGFL 244
>gb|CD662781.1|CD662781 UCRHV18_03ad10_b1 Drought-stressed Dicktoo barley epidermis cDNA
library Hordeum vulgare subsp. vulgare cDNA clone
UCRHV18_03ad10, mRNA sequence
Length = 899
Score = 82.2 bits (173), Expect(4) = 4e-025
Identities = 34/73 (46%), Positives = 46/73 (63%)
Frame = -2 / -3
Query: 417 YXQWSDASREELIDDVRRKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLK 238
Y Q + + + + D R YL+ Y+ KA+RNGANV+GYFVW+ LD FE+ G R +
Sbjct: 597 YVQENGVASNDTLYDTERIEYLKSYMGGTLKAVRNGANVKGYFVWSFLDVFEFFAGPRSR 418
Query: 237 YGLYHVDFDTQER 199
YGLY VDF+ R
Sbjct: 417 YGLYRVDFNDDAR 379
Score = 33.6 bits (67), Expect(4) = 4e-025
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -2 / -3
Query: 495 PEAIELAIQYVNGRYKGTPVYITENG 418
PE ++ +QY+ Y P+Y+ ENG
Sbjct: 657 PEGLQYVLQYLTKAYGNIPIYVQENG 580
Score = 29.9 bits (59), Expect(4) = 4e-025
Identities = 9/26 (34%), Positives = 18/26 (69%)
Frame = -2 / -3
Query: 696 EEKALLLRYKADFIGLNHYTAIYARD 619
+ ++ +++ DFIG+NHY ++Y D
Sbjct: 849 KSQSQVVKGAVDFIGINHYYSMYVND 772
Score = 28.1 bits (55), Expect(4) = 4e-025
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = -2 / -3
Query: 213 DTQERTXRMSARWYQGFL 160
D + R ++ ARWY GFL
Sbjct: 387 DARPRQAKLFARWYSGFL 334
>gb|AV919161.1|AV919161 AV919161 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags4m17 3', mRNA sequence
Length = 714
Score = 108 bits (231), Expect(2) = 6e-025
Identities = 43/113 (38%), Positives = 66/113 (58%)
Frame = -2 / -3
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
+G IG + VP + + Y++ +Y P+ ITENG Q +R++ + D
Sbjct: 550 NGKPIGPKANSNWLYIVPSGMYGCVHYLSQKYGNPPIVITENGMDQPGGLTRDQYLRDTT 371
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDT 208
R + + Y++ L KA+ GANV GYF W+LLDNFEW GY K+G+ +VDF++
Sbjct: 370 RVRFYRSYLSELKKAIDGGANVLGYFAWSLLDNFEWMSGYSSKFGIVYVDFNS 212
Score = 25.4 bits (49), Expect(2) = 6e-025
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = -2 / -2
Query: 690 KALLLRYKADFIGLNHYTA 634
+A ++ AD+IG+N YTA
Sbjct: 692 EAKMVMGSADYIGINQYTA 636
>gb|BJ467710.1|BJ467710 BJ467710 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags29i09 3', mRNA sequence
Length = 712
Score = 108 bits (231), Expect(2) = 6e-025
Identities = 43/113 (38%), Positives = 66/113 (58%)
Frame = -2 / -2
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
+G IG + VP + + Y++ +Y P+ ITENG Q +R++ + D
Sbjct: 546 NGKPIGPKANSNWLYIVPSGMYGCVHYLSQKYGNPPIVITENGMDQPGGLTRDQYLRDTT 367
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDT 208
R + + Y++ L KA+ GANV GYF W+LLDNFEW GY K+G+ +VDF++
Sbjct: 366 RVRFYRSYLSELKKAIDGGANVLGYFAWSLLDNFEWMSGYSSKFGIVYVDFNS 208
Score = 25.4 bits (49), Expect(2) = 6e-025
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = -2 / -2
Query: 690 KALLLRYKADFIGLNHYTA 634
+A ++ AD+IG+N YTA
Sbjct: 687 EAKMVMGSADYIGINQYTA 631
>gb|AV917900.1|AV917900 AV917900 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags17a02 3', mRNA sequence
Length = 691
Score = 108 bits (231), Expect(2) = 6e-025
Identities = 43/113 (38%), Positives = 66/113 (58%)
Frame = -2 / -1
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
+G IG + VP + + Y++ +Y P+ ITENG Q +R++ + D
Sbjct: 529 NGKPIGPKANSNWLYIVPSGMYGCVHYLSQKYGNPPIVITENGMDQPGGLTRDQYLRDTT 350
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDT 208
R + + Y++ L KA+ GANV GYF W+LLDNFEW GY K+G+ +VDF++
Sbjct: 349 RVRFYRSYLSELKKAIDGGANVLGYFAWSLLDNFEWMSGYSSKFGIVYVDFNS 191
Score = 25.4 bits (49), Expect(2) = 6e-025
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = -2 / -1
Query: 690 KALLLRYKADFIGLNHYTA 634
+A ++ AD+IG+N YTA
Sbjct: 670 EAKMVMGSADYIGINQYTA 614
>gb|BJ468501.1|BJ468501 BJ468501 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags39b11 3', mRNA sequence
Length = 688
Score = 108 bits (231), Expect(2) = 6e-025
Identities = 43/113 (38%), Positives = 66/113 (58%)
Frame = -2 / -1
Query: 546 DGVKIGGDTALAGFFDVPEAIELAIQYVNGRYKGTPVYITENGYXQWSDASREELIDDVR 367
+G IG + VP + + Y++ +Y P+ ITENG Q +R++ + D
Sbjct: 529 NGKPIGPKANSNWLYIVPSGMYGCVHYLSQKYGNPPIVITENGMDQPGGLTRDQYLRDTT 350
Query: 366 RKNYLQGYITYLSKALRNGANVRGYFVWTLLDNFEWAFGYRLKYGLYHVDFDT 208
R + + Y++ L KA+ GANV GYF W+LLDNFEW GY K+G+ +VDF++
Sbjct: 349 RVRFYRSYLSELKKAIDGGANVLGYFAWSLLDNFEWMSGYSSKFGIVYVDFNS 191
Score = 25.4 bits (49), Expect(2) = 6e-025
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = -2 / -1
Query: 690 KALLLRYKADFIGLNHYTA 634
+A ++ AD+IG+N YTA
Sbjct: 670 EAKMVMGSADYIGINQYTA 614
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 328,448,938
Number of Sequences: 312970
Number of extensions: 4605457
Number of successful extensions: 67858
Number of sequences better than 1.0e-020: 156
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 9052303885
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)