TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 5110719.2.1
(581 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV916139.1|AV916139 AV916139 K. Sato unpublished cDNA li... 250 2e-072
gb|BM816998.1|BM816998 HB107H12_SK.ab1 HB Hordeum vulgare s... 124 2e-037
gb|BJ477170.1|BJ477170 BJ477170 K. Sato unpublished cDNA li... 124 8e-035
gb|AV914721.1|AV914721 AV914721 K. Sato unpublished cDNA li... 124 8e-035
gb|BQ765720.1|BQ765720 EBro03_SQ008_A02_R root, 3 week, wat... 134 4e-031
gb|AL506334.1|AL506334 AL506334 Hordeum vulgare Barke devel... 133 6e-031
gb|BU998550.1|BU998550 HI11G16r HI Hordeum vulgare subsp. v... 133 6e-031
gb|BJ475515.1|BJ475515 BJ475515 K. Sato unpublished cDNA li... 100 3e-027
gb|BE420887.1|BE420887 HWM003.E12 ITEC HWM Barley Leaf Libr... 98 6e-027
gb|BF627268.3|BF627268 HVSMEb0004G12f Hordeum vulgare seedl... 95 1e-026
gb|BQ469442.1|BQ469442 HM04L11r HM Hordeum vulgare subsp. v... 66 1e-023
gb|BJ453003.1|BJ453003 BJ453003 K. Sato unpublished cDNA li... 96 2e-023
gb|BM099579.2|BM099579 EBes01_SQ003_K13_R embryo sac, 4-6 D... 96 4e-022
>gb|AV916139.1|AV916139 AV916139 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags1l14 5', mRNA sequence
Length = 642
Score = 250 bits (540), Expect(2) = 2e-072
Identities = 113/157 (71%), Positives = 120/157 (76%)
Frame = -2 / -3
Query: 472 SP*QVLLVASVRSPVSLLEAPT*VGDSDAGAGVEGSVRSESGN*SLDTVTEAPPAGVSDF 293
S * LL+ASV P+SLL+A * DSD GAGVEGSV SESGN*SLD VT AP GVSDF
Sbjct: 544 SS*HGLLIASVWLPLSLLKAAP*DADSDVGAGVEGSVLSESGN*SLDAVTVAPAPGVSDF 365
Query: 292 TPVKRCCSSMIFWRYFP*ASIRXXXXXXXXXXXXSRFWTSICSLRASDICNPEDPSSPAS 113
P KRCCSSMIF RYFP ASIR +RFWTSICS RASDIC PEDPSSPA+
Sbjct: 364 IPPKRCCSSMIFLRYFPCASIRSCSCLCTSSCSCNRFWTSICSFRASDICIPEDPSSPAN 185
Query: 112 KSPGSLLSVLFPSVEASGIYFASLYFCK*LFTWYIVN 2
+SPGSLLS L PS E SG+YFASLYFCK*LFTWYIVN
Sbjct: 184 ESPGSLLSALLPSAEESGMYFASLYFCK*LFTWYIVN 74
Score = 187 bits (402), Expect(2) = 4e-050
Identities = 89/155 (57%), Positives = 108/155 (69%)
Frame = +2 / +2
Query: 2 INNIPCKKSLAEIQTSKVYS*CFN*WEQDR*QRPRRFACRT*GILWIADI*SPQTADGGP 181
INNIPC+KSLAEIQ SKV+S F+*W Q *QR R F RT* ILW ADI*SP+ ADGGP
Sbjct: 74 INNIPCEKSLAEIQASKVHSRLFS*W*QG**QRSRGFISRT*WILWNADI*SPEAADGGP 253
Query: 182 KAAARTVRSTKAAAVTN*GSGKVPPEDHRRAAALNRCEI*NSCWRCFCHCIK*SIPRLGA 361
KA A T RSTKAAA N G+GKVP E+HRRA A ++*N+ R +CH ++ S+P L
Sbjct: 254 KAVA*TARSTKAAAAANRGTGKVP*ENHRRATAFRWYKV*NTWCRSYCHSVERSVP*L*E 433
Query: 362 D*ALHTCTSIRVSNSSWCFQ*GHWRPN*SNQEHLS 466
D +L +CT I + S FQ W+P+ SNQE +S
Sbjct: 434 DRSLDSCTHI*ICISGCSFQKRQWKPDRSNQESMS 538
Score = 133 bits (286), Expect(3) = 1e-064
Identities = 55/61 (90%), Positives = 58/61 (95%)
Frame = +3 / +3
Query: 3 LTIYHVKSHLQKYRLAKYIPDASTDGNKTDNKDPGDLLAGLEGSSGLQISEALKLQMEVQ 182
LTIYHVKSHLQKYRLAKYIPD+S DGNK DNKDPGD LAGL+GSSG+QISEALKLQMEVQ
Sbjct: 75 LTIYHVKSHLQKYRLAKYIPDSSADGNKADNKDPGDSLAGLDGSSGMQISEALKLQMEVQ 254
Query: 183 K 185
K
Sbjct: 255 K 257
Score = 129 bits (277), Expect(3) = 4e-042
Identities = 58/83 (69%), Positives = 61/83 (73%)
Frame = -3 / -1
Query: 252 GTFPEPQFVTAAAFVLLTVRAAAFGPPSAV*GLQISAIQRIPQVRQANLLGLCYRSCSHQ 73
GTFP P+F AAAFVL V A AFGPPSA GLQISA QRI QV N L LCY+ C HQ
Sbjct: 324 GTFPVPRFAAAAAFVLRAVHATAFGPPSAASGLQISAFQRIHQVLLMNPLDLCYQPCYHQ 145
Query: 72 LKHQEYTLLVCISASDFLHGILL 4
LK +E TLL CISASDF HGILL
Sbjct: 144 LKSRECTLLACISASDFSHGILL 76
Score = 125 bits (268), Expect(2) = 4e-031
Identities = 56/73 (76%), Positives = 58/73 (79%)
Frame = +1 / +1
Query: 4 *QYTM*KVTCRNTD*QSIFLMLQLMGTRPITKTQEICLPDLRDPLDCRYLKPSNCRWRSK 183
*QYTM*KVTCRNT *QS F LQLM TR ITK Q I DL DPL+CRYLKP +CRWRSK
Sbjct: 76 *QYTM*KVTCRNTG*QSTFPTLQLMVTRLITKIQGIH*QDLMDPLECRYLKP*SCRWRSK 255
Query: 184 SGCTNS*KYKGSC 222
SGC NS KYKGSC
Sbjct: 256 SGCMNSSKYKGSC 294
Score = 116 bits (247), Expect(2) = 2e-029
Identities = 50/69 (72%), Positives = 57/69 (82%)
Frame = -1 / -2
Query: 209 YF*LFVQPLLDLHLQFEGFRYLQSRGSLKSGKQISWVFVIGLVPIS*SIRNILC*SVFLQ 30
YF LF+QPLLDLHLQ +GFRYL SRGS+KS I W+FVI LV IS* + N+LC* VFLQ
Sbjct: 281 YFELFMQPLLDLHLQLQGFRYLHSRGSIKSC**IPWIFVISLVTIS*RVGNVLC*PVFLQ 102
Query: 29 VTFYMVYC* 3
VTF+MVYC*
Sbjct: 101 VTFHMVYC* 75
Score = 109 bits (232), Expect(3) = 1e-064
Identities = 47/81 (58%), Positives = 53/81 (65%)
Frame = +3 / +3
Query: 237 AQGKYLQKIIEEQQRLTGVKSETPAGGASVTVSSDQFPDSERXXXXXXXXXXXXXXQVGA 416
AQGKYL+KIIEEQQR G+KSETP GA+VT SSDQFPDSER Q A
Sbjct: 309 AQGKYLKKIIEEQQRFGGIKSETPGAGATVTASSDQFPDSERTDPSTPAPTSESASQGAA 488
Query: 417 SNRDTGDRTEATKSTCHGDSL 479
RD+G +TEA KS CH + L
Sbjct: 489 FKRDSGSQTEAIKSPCHDEPL 551
Score = 52.4 bits (108), Expect(3) = 1e-064
Identities = 21/28 (75%), Positives = 21/28 (75%)
Frame = +3 / +3
Query: 495 LTPDSNCQNGSPVASPNHERAAKRQRGS 578
LT DSNC GSP SP HERAAKRQR S
Sbjct: 552 LTADSNCHPGSPTLSPKHERAAKRQRSS 635
Score = 45.5 bits (93), Expect(3) = 4e-042
Identities = 21/47 (44%), Positives = 25/47 (53%)
Frame = -1 / -2
Query: 479 KRITMTGALGCFSSVSSVPIGSTNLSWRLGCWCRCGGLSPLRVWELI 339
+R +T L CF S+V S L R C CR G+ PLRV ELI
Sbjct: 551 QRFIVTWTLDCFGLASTVSFESCTLRCRFRCGCRSRGICPLRVRELI 411
Score = 41.8 bits (85), Expect(2) = 2e-072
Identities = 20/28 (71%), Positives = 20/28 (71%)
Frame = -2 / -3
Query: 577 LPRCLLAALSWFGLATGEPFWQLESGVS 494
L RCL AALS FGL GEP QLES VS
Sbjct: 634 LLRCLFAALSCFGLRVGEPG*QLESAVS 551
Score = 34.1 bits (68), Expect(3) = 4e-042
Identities = 14/24 (58%), Positives = 17/24 (70%)
Frame = -1 / -2
Query: 575 ASLPLGCPFMVWTSDRRTVLAVGI 504
ASLPL CP ++ T RRT + VGI
Sbjct: 632 ASLPLCCPLVLRTESRRTGMTVGI 561
Score = 33.6 bits (67), Expect(2) = 2e-029
Identities = 16/29 (55%), Positives = 16/29 (55%)
Frame = -3 / -1
Query: 576 CLAASWLPFHGLD*RQENRSGSWNQGLVA 490
C AAS LP D* ENR SWN L A
Sbjct: 633 CFAASLLPSRASD*E*ENRDDSWNLLLAA 547
Score = 30.9 bits (61), Expect(2) = 4e-050
Identities = 14/24 (58%), Positives = 17/24 (70%)
Frame = +1 / +1
Query: 505 IPTARTVLLSLVQTMKGQPRGSEA 576
IPT VLL V++ +GQ RGSEA
Sbjct: 562 IPTVIPVLLLSVRSTRGQQRGSEA 633
Score = 29.9 bits (59), Expect(2) = 4e-031
Identities = 15/29 (51%), Positives = 19/29 (65%)
Frame = +2 / +2
Query: 491 ATNP*FQLPERFSCR*SKP*KGSQEAARQ 577
A N FQL RFS S+ +GS+EAA+Q
Sbjct: 548 AANSRFQLSSRFSYSQSEAREGSKEAAKQ 634
>gb|BM816998.1|BM816998 HB107H12_SK.ab1 HB Hordeum vulgare subsp. vulgare cDNA clone
HB107H12_SK.ab1 similar to (AC006837) transfactor-like
protein [Arabidopsis thaliana], mRNA sequence
Length = 754
Score = 124 bits (266), Expect(3) = 2e-037
Identities = 60/82 (73%), Positives = 63/82 (76%)
Frame = -2 / -3
Query: 472 SP*QVLLVASVRSPVSLLEAPT*VGDSDAGAGVEGSVRSESGN*SLDTVTEAPPAGVSDF 293
S * LL+ASV P+SLL+A * DSD GAGVEGSV SESGN*SLD VT AP GVSDF
Sbjct: 296 SS*HGLLIASVWLPLSLLKAAP*DADSDVGAGVEGSVLSESGN*SLDAVTVAPAPGVSDF 117
Query: 292 TPVKRCCSSMIFWRYFP*ASIR 227
P KRCCSSMIF RYFP ASIR
Sbjct: 116 IPPKRCCSSMIFLRYFPCASIR 51
Score = 109 bits (232), Expect(2) = 1e-031
Identities = 47/81 (58%), Positives = 53/81 (65%)
Frame = +3 / +1
Query: 237 AQGKYLQKIIEEQQRLTGVKSETPAGGASVTVSSDQFPDSERXXXXXXXXXXXXXXQVGA 416
AQGKYL+KIIEEQQR G+KSETP GA+VT SSDQFPDSER Q A
Sbjct: 61 AQGKYLKKIIEEQQRFGGIKSETPGAGATVTASSDQFPDSERTDPSTPAPTSESASQGAA 240
Query: 417 SNRDTGDRTEATKSTCHGDSL 479
RD+G +TEA KS CH + L
Sbjct: 241 FKRDSGSQTEAIKSPCHDEPL 303
Score = 52.4 bits (108), Expect(2) = 1e-031
Identities = 21/28 (75%), Positives = 21/28 (75%)
Frame = +3 / +1
Query: 495 LTPDSNCQNGSPVASPNHERAAKRQRGS 578
LT DSNC GSP SP HERAAKRQR S
Sbjct: 304 LTADSNCHPGSPTLSPKHERAAKRQRSS 387
Score = 41.8 bits (85), Expect(3) = 2e-037
Identities = 20/28 (71%), Positives = 20/28 (71%)
Frame = -2 / -3
Query: 577 LPRCLLAALSWFGLATGEPFWQLESGVS 494
L RCL AALS FGL GEP QLES VS
Sbjct: 386 LLRCLFAALSCFGLRVGEPG*QLESAVS 303
Score = 27.2 bits (53), Expect(3) = 2e-037
Identities = 11/23 (47%), Positives = 13/23 (56%)
Frame = -3 / -1
Query: 252 GTFPEPQFVTAAAFVLLTVRAAA 184
GTFP P+F AAA + R A
Sbjct: 76 GTFPVPRFAAAAALYFASCRIPA 8
>gb|BJ477170.1|BJ477170 BJ477170 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal40f15 3', mRNA sequence
Length = 661
Score = 124 bits (266), Expect(2) = 8e-035
Identities = 60/82 (73%), Positives = 63/82 (76%)
Frame = -2 / +3
Query: 472 SP*QVLLVASVRSPVSLLEAPT*VGDSDAGAGVEGSVRSESGN*SLDTVTEAPPAGVSDF 293
S * LL+ASV P+SLL+A * DSD GAGVEGSV SESGN*SLD VT AP GVSDF
Sbjct: 402 SS*HGLLIASVWLPLSLLKAAP*DADSDVGAGVEGSVLSESGN*SLDAVTVAPAPGVSDF 581
Query: 292 TPVKRCCSSMIFWRYFP*ASIR 227
P KRCCSSMIF RYFP ASIR
Sbjct: 582 IPPKRCCSSMIFLRYFPCASIR 647
Score = 109 bits (232), Expect(2) = 1e-031
Identities = 47/81 (58%), Positives = 53/81 (65%)
Frame = +3 / -1
Query: 237 AQGKYLQKIIEEQQRLTGVKSETPAGGASVTVSSDQFPDSERXXXXXXXXXXXXXXQVGA 416
AQGKYL+KIIEEQQR G+KSETP GA+VT SSDQFPDSER Q A
Sbjct: 637 AQGKYLKKIIEEQQRFGGIKSETPGAGATVTASSDQFPDSERTDPSTPAPTSESASQGAA 458
Query: 417 SNRDTGDRTEATKSTCHGDSL 479
RD+G +TEA KS CH + L
Sbjct: 457 FKRDSGSQTEAIKSPCHDEPL 395
Score = 52.4 bits (108), Expect(2) = 1e-031
Identities = 21/28 (75%), Positives = 21/28 (75%)
Frame = +3 / -1
Query: 495 LTPDSNCQNGSPVASPNHERAAKRQRGS 578
LT DSNC GSP SP HERAAKRQR S
Sbjct: 394 LTADSNCHPGSPTLSPKHERAAKRQRSS 311
Score = 41.8 bits (85), Expect(2) = 8e-035
Identities = 20/28 (71%), Positives = 20/28 (71%)
Frame = -2 / +3
Query: 577 LPRCLLAALSWFGLATGEPFWQLESGVS 494
L RCL AALS FGL GEP QLES VS
Sbjct: 312 LLRCLFAALSCFGLRVGEPG*QLESAVS 395
>gb|AV914721.1|AV914721 AV914721 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags7m02 5', mRNA sequence
Length = 524
Score = 124 bits (266), Expect(2) = 8e-035
Identities = 60/82 (73%), Positives = 63/82 (76%)
Frame = -2 / -1
Query: 472 SP*QVLLVASVRSPVSLLEAPT*VGDSDAGAGVEGSVRSESGN*SLDTVTEAPPAGVSDF 293
S * LL+ASV P+SLL+A * DSD GAGVEGSV SESGN*SLD VT AP GVSDF
Sbjct: 278 SS*HGLLIASVWLPLSLLKAAP*DADSDVGAGVEGSVLSESGN*SLDAVTVAPAPGVSDF 99
Query: 292 TPVKRCCSSMIFWRYFP*ASIR 227
P KRCCSSMIF RYFP ASIR
Sbjct: 98 IPPKRCCSSMIFLRYFPCASIR 33
Score = 109 bits (232), Expect(2) = 1e-031
Identities = 47/81 (58%), Positives = 53/81 (65%)
Frame = +3 / +1
Query: 237 AQGKYLQKIIEEQQRLTGVKSETPAGGASVTVSSDQFPDSERXXXXXXXXXXXXXXQVGA 416
AQGKYL+KIIEEQQR G+KSETP GA+VT SSDQFPDSER Q A
Sbjct: 43 AQGKYLKKIIEEQQRFGGIKSETPGAGATVTASSDQFPDSERTDPSTPAPTSESASQGAA 222
Query: 417 SNRDTGDRTEATKSTCHGDSL 479
RD+G +TEA KS CH + L
Sbjct: 223 FKRDSGSQTEAIKSPCHDEPL 285
Score = 88.6 bits (187), Expect(2) = 9e-021
Identities = 41/87 (47%), Positives = 56/87 (64%)
Frame = +2 / +3
Query: 206 STKAAAVTN*GSGKVPPEDHRRAAALNRCEI*NSCWRCFCHCIK*SIPRLGAD*ALHTCT 385
STKAAA N G+GKVP E+HRRA A ++*N+ R +CH ++ S+P L D +L +CT
Sbjct: 12 STKAAAAANRGTGKVP*ENHRRATAFRWYKV*NTWCRSYCHSVERSVP*L*EDRSLDSCT 191
Query: 386 SIRVSNSSWCFQ*GHWRPN*SNQEHLS 466
I + S FQ W+P+ SNQE +S
Sbjct: 192 HI*ICISGCSFQKRQWKPDRSNQESMS 272
Score = 52.4 bits (108), Expect(2) = 1e-031
Identities = 21/28 (75%), Positives = 21/28 (75%)
Frame = +3 / +1
Query: 495 LTPDSNCQNGSPVASPNHERAAKRQRGS 578
LT DSNC GSP SP HERAAKRQR S
Sbjct: 286 LTADSNCHPGSPTLSPKHERAAKRQRSS 369
Score = 41.8 bits (85), Expect(2) = 8e-035
Identities = 20/28 (71%), Positives = 20/28 (71%)
Frame = -2 / -1
Query: 577 LPRCLLAALSWFGLATGEPFWQLESGVS 494
L RCL AALS FGL GEP QLES VS
Sbjct: 368 LLRCLFAALSCFGLRVGEPG*QLESAVS 285
Score = 30.9 bits (61), Expect(2) = 9e-021
Identities = 14/24 (58%), Positives = 17/24 (70%)
Frame = +1 / +2
Query: 505 IPTARTVLLSLVQTMKGQPRGSEA 576
IPT VLL V++ +GQ RGSEA
Sbjct: 296 IPTVIPVLLLSVRSTRGQQRGSEA 367
>gb|BQ765720.1|BQ765720 EBro03_SQ008_A02_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ008_A02 5', mRNA sequence
Length = 626
Score = 134 bits (287), Expect = 4e-031
Identities = 56/78 (71%), Positives = 60/78 (76%)
Frame = -2 / -1
Query: 235 SIRXXXXXXXXXXXXSRFWTSICSLRASDICNPEDPSSPASKSPGSLLSVLFPSVEASGI 56
SIR +RFWTSICS RASDIC PEDPSSPA++SPGSLLS L PS E SG+
Sbjct: 626 SIRSCSCLCTSSCSCNRFWTSICSFRASDICIPEDPSSPANESPGSLLSALLPSAEESGM 447
Query: 55 YFASLYFCK*LFTWYIVN 2
YFASLYFCK*LFTWYIVN
Sbjct: 446 YFASLYFCK*LFTWYIVN 393
Score = 133 bits (286), Expect = 1e-029
Identities = 55/61 (90%), Positives = 58/61 (95%)
Frame = +3 / +1
Query: 3 LTIYHVKSHLQKYRLAKYIPDASTDGNKTDNKDPGDLLAGLEGSSGLQISEALKLQMEVQ 182
LTIYHVKSHLQKYRLAKYIPD+S DGNK DNKDPGD LAGL+GSSG+QISEALKLQMEVQ
Sbjct: 394 LTIYHVKSHLQKYRLAKYIPDSSADGNKADNKDPGDSLAGLDGSSGMQISEALKLQMEVQ 573
Query: 183 K 185
K
Sbjct: 574 K 576
Score = 128 bits (274), Expect = 3e-029
Identities = 57/74 (77%), Positives = 59/74 (79%)
Frame = +1 / +2
Query: 1 D*QYTM*KVTCRNTD*QSIFLMLQLMGTRPITKTQEICLPDLRDPLDCRYLKPSNCRWRS 180
D*QYTM*KVTCRNT *QS F LQLM TR ITK Q I DL DPL+CRYLKP +CRWRS
Sbjct: 392 D*QYTM*KVTCRNTG*QSTFPTLQLMVTRLITKIQGIH*QDLMDPLECRYLKP*SCRWRS 571
Query: 181 KSGCTNS*KYKGSC 222
KSGC NS KYKGSC
Sbjct: 572 KSGCMNSSKYKGSC 613
Score = 118 bits (252), Expect = 3e-026
Identities = 54/78 (69%), Positives = 57/78 (73%)
Frame = -3 / -2
Query: 234 QFVTAAAFVLLTVRAAAFGPPSAV*GLQISAIQRIPQVRQANLLGLCYRSCSHQLKHQEY 55
+F AAAFVL V A AFGPPSA GLQISA QRI QV N L LCY+ C HQLK +E
Sbjct: 625 RFAAAAAFVLRAVHATAFGPPSAASGLQISAFQRIHQVLLMNPLDLCYQPCYHQLKSREC 446
Query: 54 TLLVCISASDFLHGILLI 1
TLL CISASDF HGILLI
Sbjct: 445 TLLACISASDFSHGILLI 392
Score = 116 bits (247), Expect = 1e-025
Identities = 55/77 (71%), Positives = 59/77 (76%)
Frame = +2 / +3
Query: 2 INNIPCKKSLAEIQTSKVYS*CFN*WEQDR*QRPRRFACRT*GILWIADI*SPQTADGGP 181
INNIPC+KSLAEIQ SKV+S F+*W Q *QR R F RT* ILW ADI*SP+ ADGGP
Sbjct: 393 INNIPCEKSLAEIQASKVHSRLFS*W*QG**QRSRGFISRT*WILWNADI*SPEAADGGP 572
Query: 182 KAAARTVRSTKAAAVTN 232
KA A T RSTKAAA N
Sbjct: 573 KAVA*TARSTKAAAAAN 623
Score = 116 bits (247), Expect = 1e-025
Identities = 50/69 (72%), Positives = 57/69 (82%)
Frame = -1 / -3
Query: 209 YF*LFVQPLLDLHLQFEGFRYLQSRGSLKSGKQISWVFVIGLVPIS*SIRNILC*SVFLQ 30
YF LF+QPLLDLHLQ +GFRYL SRGS+KS I W+FVI LV IS* + N+LC* VFLQ
Sbjct: 600 YFELFMQPLLDLHLQLQGFRYLHSRGSIKSC**IPWIFVISLVTIS*RVGNVLC*PVFLQ 421
Query: 29 VTFYMVYC* 3
VTF+MVYC*
Sbjct: 420 VTFHMVYC* 394
>gb|AL506334.1|AL506334 AL506334 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY02M13T 5',
mRNA sequence
Length = 700
Score = 133 bits (286), Expect = 1e-029
Identities = 55/61 (90%), Positives = 58/61 (95%)
Frame = +3 / +3
Query: 3 LTIYHVKSHLQKYRLAKYIPDASTDGNKTDNKDPGDLLAGLEGSSGLQISEALKLQMEVQ 182
LTIYHVKSHLQKYRLAKYIPD+S DGNK DNKDPGD LAGL+GSSG+QISEALKLQMEVQ
Sbjct: 474 LTIYHVKSHLQKYRLAKYIPDSSADGNKADNKDPGDSLAGLDGSSGMQISEALKLQMEVQ 653
Query: 183 K 185
K
Sbjct: 654 K 656
Score = 133 bits (286), Expect = 6e-031
Identities = 53/62 (85%), Positives = 56/62 (90%)
Frame = -2 / -1
Query: 187 RFWTSICSLRASDICNPEDPSSPASKSPGSLLSVLFPSVEASGIYFASLYFCK*LFTWYI 8
RFWTSICS RASDIC PEDPSSPA++SPGSLLS L PS E SG+YFASLYFCK*LFTWYI
Sbjct: 658 RFWTSICSFRASDICIPEDPSSPANESPGSLLSALLPSAEESGMYFASLYFCK*LFTWYI 479
Query: 7 VN 2
VN
Sbjct: 478 VN 473
Score = 114 bits (243), Expect = 5e-025
Identities = 52/69 (75%), Positives = 54/69 (78%)
Frame = +1 / +1
Query: 1 D*QYTM*KVTCRNTD*QSIFLMLQLMGTRPITKTQEICLPDLRDPLDCRYLKPSNCRWRS 180
D*QYTM*KVTCRNT *QS F LQLM TR ITK Q I DL DPL+CRYLKP +CRWRS
Sbjct: 472 D*QYTM*KVTCRNTG*QSTFPTLQLMVTRLITKIQGIH*QDLMDPLECRYLKP*SCRWRS 651
Query: 181 KSGCTNS*K 207
KSGC NS K
Sbjct: 652 KSGCMNSSK 678
Score = 112 bits (240), Expect = 1e-024
Identities = 49/68 (72%), Positives = 56/68 (82%)
Frame = -1 / -3
Query: 206 F*LFVQPLLDLHLQFEGFRYLQSRGSLKSGKQISWVFVIGLVPIS*SIRNILC*SVFLQV 27
F LF+QPLLDLHLQ +GFRYL SRGS+KS I W+FVI LV IS* + N+LC* VFLQV
Sbjct: 677 FELFMQPLLDLHLQLQGFRYLHSRGSIKSC**IPWIFVISLVTIS*RVGNVLC*PVFLQV 498
Query: 26 TFYMVYC* 3
TF+MVYC*
Sbjct: 497 TFHMVYC* 474
Score = 104 bits (221), Expect = 5e-022
Identities = 47/66 (71%), Positives = 49/66 (74%)
Frame = -3 / -2
Query: 198 VRAAAFGPPSAV*GLQISAIQRIPQVRQANLLGLCYRSCSHQLKHQEYTLLVCISASDFL 19
V A AFGPPSA GLQISA QRI QV N L LCY+ C HQLK +E TLL CISASDF
Sbjct: 669 VHATAFGPPSAASGLQISAFQRIHQVLLMNPLDLCYQPCYHQLKSRECTLLACISASDFS 490
Query: 18 HGILLI 1
HGILLI
Sbjct: 489 HGILLI 472
Score = 102 bits (218), Expect = 1e-021
Identities = 49/71 (69%), Positives = 53/71 (74%)
Frame = +2 / +2
Query: 2 INNIPCKKSLAEIQTSKVYS*CFN*WEQDR*QRPRRFACRT*GILWIADI*SPQTADGGP 181
INNIPC+KSLAEIQ SKV+S F+*W Q *QR R F RT* ILW ADI*SP+ ADGGP
Sbjct: 473 INNIPCEKSLAEIQASKVHSRLFS*W*QG**QRSRGFISRT*WILWNADI*SPEAADGGP 652
Query: 182 KAAARTVRSTK 214
KA A T R K
Sbjct: 653 KAVA*TARKYK 685
>gb|BU998550.1|BU998550 HI11G16r HI Hordeum vulgare subsp. vulgare cDNA clone HI11G16
5-PRIME, mRNA sequence
Length = 625
Score = 133 bits (286), Expect = 1e-029
Identities = 55/61 (90%), Positives = 58/61 (95%)
Frame = +3 / +1
Query: 3 LTIYHVKSHLQKYRLAKYIPDASTDGNKTDNKDPGDLLAGLEGSSGLQISEALKLQMEVQ 182
LTIYHVKSHLQKYRLAKYIPD+S DGNK DNKDPGD LAGL+GSSG+QISEALKLQMEVQ
Sbjct: 394 LTIYHVKSHLQKYRLAKYIPDSSADGNKADNKDPGDSLAGLDGSSGMQISEALKLQMEVQ 573
Query: 183 K 185
K
Sbjct: 574 K 576
Score = 133 bits (286), Expect = 6e-031
Identities = 53/62 (85%), Positives = 56/62 (90%)
Frame = -2 / -3
Query: 187 RFWTSICSLRASDICNPEDPSSPASKSPGSLLSVLFPSVEASGIYFASLYFCK*LFTWYI 8
RFWTSICS RASDIC PEDPSSPA++SPGSLLS L PS E SG+YFASLYFCK*LFTWYI
Sbjct: 578 RFWTSICSFRASDICIPEDPSSPANESPGSLLSALLPSAEESGMYFASLYFCK*LFTWYI 399
Query: 7 VN 2
VN
Sbjct: 398 VN 393
Score = 128 bits (274), Expect = 3e-029
Identities = 57/74 (77%), Positives = 59/74 (79%)
Frame = +1 / +2
Query: 1 D*QYTM*KVTCRNTD*QSIFLMLQLMGTRPITKTQEICLPDLRDPLDCRYLKPSNCRWRS 180
D*QYTM*KVTCRNT *QS F LQLM TR ITK Q I DL DPL+CRYLKP +CRWRS
Sbjct: 392 D*QYTM*KVTCRNTG*QSTFPTLQLMVTRLITKIQGIH*QDLMDPLECRYLKP*SCRWRS 571
Query: 181 KSGCTNS*KYKGSC 222
KSGC NS KYKGSC
Sbjct: 572 KSGCMNSSKYKGSC 613
Score = 118 bits (252), Expect = 3e-026
Identities = 54/78 (69%), Positives = 57/78 (73%)
Frame = -3 / -1
Query: 234 QFVTAAAFVLLTVRAAAFGPPSAV*GLQISAIQRIPQVRQANLLGLCYRSCSHQLKHQEY 55
+F AAAFVL V A AFGPPSA GLQISA QRI QV N L LCY+ C HQLK +E
Sbjct: 625 RFAAAAAFVLRAVHATAFGPPSAASGLQISAFQRIHQVLLMNPLDLCYQPCYHQLKSREC 446
Query: 54 TLLVCISASDFLHGILLI 1
TLL CISASDF HGILLI
Sbjct: 445 TLLACISASDFSHGILLI 392
Score = 116 bits (247), Expect = 1e-025
Identities = 55/77 (71%), Positives = 59/77 (76%)
Frame = +2 / +3
Query: 2 INNIPCKKSLAEIQTSKVYS*CFN*WEQDR*QRPRRFACRT*GILWIADI*SPQTADGGP 181
INNIPC+KSLAEIQ SKV+S F+*W Q *QR R F RT* ILW ADI*SP+ ADGGP
Sbjct: 393 INNIPCEKSLAEIQASKVHSRLFS*W*QG**QRSRGFISRT*WILWNADI*SPEAADGGP 572
Query: 182 KAAARTVRSTKAAAVTN 232
KA A T RSTKAAA N
Sbjct: 573 KAVA*TARSTKAAAAAN 623
Score = 116 bits (247), Expect = 1e-025
Identities = 50/69 (72%), Positives = 57/69 (82%)
Frame = -1 / -2
Query: 209 YF*LFVQPLLDLHLQFEGFRYLQSRGSLKSGKQISWVFVIGLVPIS*SIRNILC*SVFLQ 30
YF LF+QPLLDLHLQ +GFRYL SRGS+KS I W+FVI LV IS* + N+LC* VFLQ
Sbjct: 600 YFELFMQPLLDLHLQLQGFRYLHSRGSIKSC**IPWIFVISLVTIS*RVGNVLC*PVFLQ 421
Query: 29 VTFYMVYC* 3
VTF+MVYC*
Sbjct: 420 VTFHMVYC* 394
>gb|BJ475515.1|BJ475515 BJ475515 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal26b02 3', mRNA sequence
Length = 604
Score = 99.6 bits (211), Expect(2) = 3e-027
Identities = 48/68 (70%), Positives = 51/68 (75%)
Frame = -2 / +1
Query: 472 SP*QVLLVASVRSPVSLLEAPT*VGDSDAGAGVEGSVRSESGN*SLDTVTEAPPAGVSDF 293
S * LL+ASV P+SLL+A * DSD GAGVEGSV SESGN*SLD VT AP GVSDF
Sbjct: 400 SS*HGLLIASVWLPLSLLKAAP*DADSDVGAGVEGSVLSESGN*SLDAVTVAPAPGVSDF 579
Query: 292 TPVKRCCS 269
P KRCCS
Sbjct: 580 IPPKRCCS 603
Score = 86.3 bits (182), Expect(2) = 4e-025
Identities = 37/70 (52%), Positives = 42/70 (60%)
Frame = +3 / -3
Query: 270 EQQRLTGVKSETPAGGASVTVSSDQFPDSERXXXXXXXXXXXXXXQVGASNRDTGDRTEA 449
EQQR G+KSETP GA+VT SSDQFPDSER Q A RD+G +TEA
Sbjct: 602 EQQRFGGIKSETPGAGATVTASSDQFPDSERTDPSTPAPTSESASQGAAFKRDSGSQTEA 423
Query: 450 TKSTCHGDSL 479
KS CH + L
Sbjct: 422 IKSPCHDEPL 393
Score = 52.4 bits (108), Expect(2) = 4e-025
Identities = 21/28 (75%), Positives = 21/28 (75%)
Frame = +3 / -3
Query: 495 LTPDSNCQNGSPVASPNHERAAKRQRGS 578
LT DSNC GSP SP HERAAKRQR S
Sbjct: 392 LTADSNCHPGSPTLSPKHERAAKRQRSS 309
Score = 41.8 bits (85), Expect(2) = 3e-027
Identities = 20/28 (71%), Positives = 20/28 (71%)
Frame = -2 / +1
Query: 577 LPRCLLAALSWFGLATGEPFWQLESGVS 494
L RCL AALS FGL GEP QLES VS
Sbjct: 310 LLRCLFAALSCFGLRVGEPG*QLESAVS 393
>gb|BE420887.1|BE420887 HWM003.E12 ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM003.E12, mRNA sequence
Length = 845
Score = 98.2 bits (208), Expect(2) = 6e-027
Identities = 48/71 (67%), Positives = 52/71 (73%)
Frame = -2 / -2
Query: 472 SP*QVLLVASVRSPVSLLEAPT*VGDSDAGAGVEGSVRSESGN*SLDTVTEAPPAGVSDF 293
S * LL+ASV P+SLL+A * DSD GAGVEGSV SESGN*SLD VT AP GVSDF
Sbjct: 226 SS*HGLLIASVWLPLSLLKAAP*DADSDVGAGVEGSVLSESGN*SLDAVTVAPAPGVSDF 47
Query: 292 TPVKRCCSSMI 260
P KRCC +I
Sbjct: 46 IPPKRCCWCII 14
Score = 87.2 bits (184), Expect(2) = 2e-025
Identities = 37/73 (50%), Positives = 44/73 (60%)
Frame = +3 / +3
Query: 261 IIEEQQRLTGVKSETPAGGASVTVSSDQFPDSERXXXXXXXXXXXXXXQVGASNRDTGDR 440
I+ +QQR G+KSETP GA+VT SSDQFPDSER Q A RD+G +
Sbjct: 15 IMHQQQRFGGIKSETPGAGATVTASSDQFPDSERTDPSTPAPTSESASQGAAFKRDSGSQ 194
Query: 441 TEATKSTCHGDSL 479
TEA KS CH + L
Sbjct: 195 TEAIKSPCHDEPL 233
Score = 52.4 bits (108), Expect(2) = 2e-025
Identities = 21/28 (75%), Positives = 21/28 (75%)
Frame = +3 / +3
Query: 495 LTPDSNCQNGSPVASPNHERAAKRQRGS 578
LT DSNC GSP SP HERAAKRQR S
Sbjct: 234 LTADSNCHPGSPTLSPKHERAAKRQRSS 317
Score = 41.8 bits (85), Expect(2) = 6e-027
Identities = 20/28 (71%), Positives = 20/28 (71%)
Frame = -2 / -2
Query: 577 LPRCLLAALSWFGLATGEPFWQLESGVS 494
L RCL AALS FGL GEP QLES VS
Sbjct: 316 LLRCLFAALSCFGLRVGEPG*QLESAVS 233
>gb|BF627268.3|BF627268 HVSMEb0004G12f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0004G12f, mRNA sequence
Length = 875
Score = 95.5 bits (202), Expect(3) = 1e-026
Identities = 39/50 (78%), Positives = 42/50 (84%)
Frame = +3 / +1
Query: 3 LTIYHVKSHLQKYRLAKYIPDASTDGNKTDNKDPGDLLAGLEGSSGLQIS 152
LTIYHVKSHLQKYRLA YIPD+S DGNK D DPGD LAGL+GS +QIS
Sbjct: 418 LTIYHVKSHLQKYRLAXYIPDSSADGNKADXXDPGDSLAGLDGSLWMQIS 567
Score = 94.1 bits (199), Expect(2) = 1e-025
Identities = 38/52 (73%), Positives = 41/52 (78%)
Frame = -2 / -1
Query: 157 ASDICNPEDPSSPASKSPGSLLSVLFPSVEASGIYFASLYFCK*LFTWYIVN 2
A DIC DPSSPA++SPGS S L PS E SG+Y ASLYFCK*LFTWYIVN
Sbjct: 572 AQDICIQRDPSSPANESPGSXXSALLPSAEESGMYXASLYFCK*LFTWYIVN 417
Score = 41.4 bits (84), Expect(2) = 1e-025
Identities = 17/25 (68%), Positives = 18/25 (72%)
Frame = -3 / -3
Query: 246 FPEPQFVTAAAFVLLTVRAAAFGPP 172
FP P+F AAAFVL V A AFGPP
Sbjct: 663 FPWPRFAGAAAFVLRAVHATAFGPP 589
Score = 40.0 bits (81), Expect(3) = 1e-026
Identities = 18/25 (72%), Positives = 18/25 (72%)
Frame = +2 / +2
Query: 173 GGPKAAARTVRSTKAAAVTN*GSGK 247
GGPKA A T RSTKAAA N G GK
Sbjct: 590 GGPKAVA*TARSTKAAAPANRGQGK 664
Score = 24.4 bits (47), Expect(3) = 1e-026
Identities = 8/14 (57%), Positives = 9/14 (64%)
Frame = +1 / +3
Query: 133 PLDCRYLKPSNCRW 174
PLD L P +CRW
Sbjct: 549 PLDADILSP*SCRW 590
>gb|BQ469442.1|BQ469442 HM04L11r HM Hordeum vulgare subsp. vulgare cDNA clone HM04L11
5-PRIME, mRNA sequence
Length = 651
Score = 66.1 bits (138), Expect(4) = 1e-023
Identities = 26/41 (63%), Positives = 31/41 (75%)
Frame = +3 / +1
Query: 12 YHVKSHLQKYRLAKYIPDASTDGNKTDNKDPGDLLAGLEGS 134
+ VKSHLQKYRLAKYIPD + G+K + KD G LL G+E S
Sbjct: 4 HEVKSHLQKYRLAKYIPDTTASGDKPEKKDLGSLLEGIESS 126
Score = 45.5 bits (93), Expect(4) = 1e-023
Identities = 19/23 (82%), Positives = 21/23 (91%)
Frame = +3 / +1
Query: 237 AQGKYLQKIIEEQQRLTGVKSET 305
AQGKYLQKI+EEQQRLTGV E+
Sbjct: 232 AQGKYLQKIMEEQQRLTGVLCES 300
Score = 34.1 bits (68), Expect(4) = 1e-023
Identities = 12/19 (63%), Positives = 15/19 (78%)
Frame = +1 / +2
Query: 163 NCRWRSKSGCTNS*KYKGS 219
NCRWR +S N+*+YKGS
Sbjct: 158 NCRWRCRSVYVNN*RYKGS 214
Score = 24.0 bits (46), Expect(4) = 1e-023
Identities = 10/28 (35%), Positives = 14/28 (50%)
Frame = +3 / +1
Query: 498 TPDSNCQNGSPVASPNHERAAKRQRGSG 581
TPDS+C G + SP + A+ G
Sbjct: 505 TPDSSCGAGYLLNSPRDSKRARVSNNLG 588
>gb|BJ453003.1|BJ453003 BJ453003 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak41e01 5', mRNA sequence
Length = 602
Score = 96.4 bits (204), Expect(2) = 2e-023
Identities = 38/61 (62%), Positives = 49/61 (80%)
Frame = +3 / +1
Query: 3 LTIYHVKSHLQKYRLAKYIPDASTDGNKTDNKDPGDLLAGLEGSSGLQISEALKLQMEVQ 182
+TIYHVKSHLQKYRLAKYIP++ +G+K + KD D L+ + + G QI+EALK+QMEVQ
Sbjct: 43 ITIYHVKSHLQKYRLAKYIPESPAEGSKEEKKDSSDSLSNTDSAPGSQINEALKMQMEVQ 222
Query: 183 K 185
K
Sbjct: 223 K 225
Score = 36.3 bits (73), Expect(2) = 2e-023
Identities = 15/18 (83%), Positives = 16/18 (88%)
Frame = +3 / +1
Query: 237 AQGKYLQKIIEEQQRLTG 290
AQGKYLQ IIEEQQ+L G
Sbjct: 277 AQGKYLQMIIEEQQKLGG 330
>gb|BM099579.2|BM099579 EBes01_SQ003_K13_R embryo sac, 4-6 DPA, no treatment, cv Optic,
EBes01 Hordeum vulgare subsp. vulgare cDNA clone
EBes01_SQ003_K13 5', mRNA sequence
Length = 602
Score = 96.4 bits (204), Expect(2) = 4e-022
Identities = 38/61 (62%), Positives = 49/61 (80%)
Frame = +3 / +1
Query: 3 LTIYHVKSHLQKYRLAKYIPDASTDGNKTDNKDPGDLLAGLEGSSGLQISEALKLQMEVQ 182
+TIYHVKSHLQKYRLAKYIP++ +G+K + KD D L+ + + G QI+EALK+QMEVQ
Sbjct: 325 ITIYHVKSHLQKYRLAKYIPESPAEGSKEEKKDSSDSLSNTDSAPGSQINEALKMQMEVQ 504
Query: 183 K 185
K
Sbjct: 505 K 507
Score = 31.8 bits (63), Expect(2) = 4e-022
Identities = 13/14 (92%), Positives = 13/14 (92%)
Frame = +3 / +1
Query: 237 AQGKYLQKIIEEQQ 278
AQGKYLQ IIEEQQ
Sbjct: 559 AQGKYLQMIIEEQQ 600
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 288,170,722
Number of Sequences: 312970
Number of extensions: 4693822
Number of successful extensions: 69804
Number of sequences better than 1.0e-020: 19
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6023172678
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)