TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4580194.2.1
(542 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ462939.1|BQ462939 HI02H22r HI Hordeum vulgare subsp. v... 246 6e-065
gb|BQ469970.1|BQ469970 HX01G08T HX Hordeum vulgare subsp. v... 237 3e-062
gb|BF623654.2|BF623654 HVSMEa0003G14f Hordeum vulgare seedl... 237 4e-062
gb|BF621457.2|BF621457 HVSMEa0011A19f Hordeum vulgare seedl... 196 6e-052
gb|BG310318.1|BG310318 HVSMEc0017K01f Hordeum vulgare seedl... 164 3e-040
gb|BU983423.1|BU983423 HA29L02r HA Hordeum vulgare subsp. v... 164 3e-040
gb|CA029988.1|CA029988 HX05M10r HX Hordeum vulgare subsp. v... 164 3e-040
gb|CA031982.1|CA031982 HX11K19r HX Hordeum vulgare subsp. v... 164 3e-040
gb|CK122262.1|CK122262 BES1824102j21 BES1824 Hordeum vulgar... 164 3e-040
gb|BQ765072.1|BQ765072 EBca01_SQ005_P15_R carpel, pre-anthe... 131 5e-035
gb|AW982890.1|AW982890 HVSMEg0004H18f Hordeum vulgare pre-a... 131 4e-030
gb|BI958161.1|BI958161 HVSMEn0013L01f Hordeum vulgare rachi... 112 2e-028
gb|BE215083.3|BE215083 HV_CEb0005M01f Hordeum vulgare seedl... 106 7e-023
>gb|BQ462939.1|BQ462939 HI02H22r HI Hordeum vulgare subsp. vulgare cDNA clone HI02H22
5-PRIME, mRNA sequence
Length = 573
Score = 246 bits (532), Expect = 6e-065
Identities = 97/126 (76%), Positives = 104/126 (82%)
Frame = +2 / +3
Query: 164 MKRKTRNKXXXXXXXXXXXXXXXGGTIALVLTAGTWKAKIKKSQEKICNKGWECSGSKYC 343
MKRKTRNK GGT+AL+LTAGTWK K+K+SQEK+CNKGWECSG+KYC
Sbjct: 3 MKRKTRNKIVVTVLLLGAAAVLIGGTLALILTAGTWKVKLKESQEKVCNKGWECSGTKYC 182
Query: 344 CNDTITDFFKVYQFENLFAKRNTPVAHAVGFWDYQAFITAAALFEPQGFCTTGGKQMQMM 523
CN TITDFFKVYQFENLFAKRN PVA AVGFWDYQAFITAA+LFE QGFCTTGG MQMM
Sbjct: 183 CNQTITDFFKVYQFENLFAKRNFPVAKAVGFWDYQAFITAASLFENQGFCTTGGIDMQMM 362
Query: 524 ELCAFL 541
ELCAFL
Sbjct: 363 ELCAFL 380
Score = 215 bits (464), Expect = 1e-055
Identities = 94/126 (74%), Positives = 98/126 (77%)
Frame = -3 / -3
Query: 540 RKAQSSIICICLPPVVQNPWGSKRAAAVMKAW*SQNPTACATGVLRLAKRFSNWYTLKKS 361
RKAQSSIIC+ +PPVVQ PW SKR AAVMKAW*SQ PTA ATG LRLAKRFSNWYTLKKS
Sbjct: 379 RKAQSSIICMSMPPVVQKPWFSKREAAVMKAW*SQKPTALATGKLRLAKRFSNWYTLKKS 200
Query: 360 VMVSLQQYLLPEHSHPLLQIFSCDFFILAFHVPAVSTSAIVPPTRIATAARASVHTMILF 181
VMV LQQYL+PEHSHPLL FSCD FHVPAV SA VPP A A +S T ILF
Sbjct: 199 VMVWLQQYLVPEHSHPLLHTFSCDSLSFTFHVPAVRISASVPPISTAAAPSSSTVTTILF 20
Query: 180 RVFRFI 163
VFRFI
Sbjct: 19 LVFRFI 2
Score = 132 bits (283), Expect = 1e-030
Identities = 63/119 (52%), Positives = 70/119 (58%)
Frame = +1 / +2
Query: 184 QDHRMDAGPGCSGDSGGRHDCAGAHGGDVEGQDKEVAGEDL*QGVGVLGEQVXXXXXXXX 363
QD A G G + RH A GGDVEG+ + VAGE + QGVGVL +QV
Sbjct: 23 QDRGDGAAAGGGGGADRRHAGADPDGGDVEGEAQGVAGEGVQQGVGVLRDQVLLQPDHHR 202
Query: 364 XXXGVPVREPLXXXXXXXXXXXXVLGLPGLHHRRGPLRAPGVLHHRRQADADDGALRLP 540
GVPVREPL +LGLPGLHHRR PLR PG+LHHRR ADDGALRLP
Sbjct: 203 LLQGVPVREPLRQAQLPRRQGRRLLGLPGLHHRRLPLREPGLLHHRRHRHADDGALRLP 379
>gb|BQ469970.1|BQ469970 HX01G08T HX Hordeum vulgare subsp. vulgare cDNA clone HX01G08
5-PRIME, mRNA sequence
Length = 468
Score = 237 bits (513), Expect = 3e-062
Identities = 94/123 (76%), Positives = 100/123 (81%)
Frame = +2 / +1
Query: 173 KTRNKXXXXXXXXXXXXXXXGGTIALVLTAGTWKAKIKKSQEKICNKGWECSGSKYCCND 352
KTRNK GGT+AL+LTAGTWK K+K+SQEK CNKGWECSG+KYCCN
Sbjct: 13 KTRNKIVVTVLLLGAAAVLIGGTLALILTAGTWKVKLKESQEKACNKGWECSGTKYCCNQ 192
Query: 353 TITDFFKVYQFENLFAKRNTPVAHAVGFWDYQAFITAAALFEPQGFCTTGGKQMQMMELC 532
TITDFFKVYQFENLFAKRN PVA AVGFWDYQAFITAA+LFE QGFCTTGG MQMMELC
Sbjct: 193 TITDFFKVYQFENLFAKRNFPVAKAVGFWDYQAFITAASLFENQGFCTTGGIDMQMMELC 372
Query: 533 AFL 541
AFL
Sbjct: 373 AFL 381
Score = 208 bits (449), Expect = 2e-053
Identities = 91/123 (73%), Positives = 95/123 (77%)
Frame = -3 / -2
Query: 540 RKAQSSIICICLPPVVQNPWGSKRAAAVMKAW*SQNPTACATGVLRLAKRFSNWYTLKKS 361
RKAQSSIIC+ +PPVVQ PW SKR AAVMKAW*SQ PTA ATG LRLAKRFSNWYTLKKS
Sbjct: 380 RKAQSSIICMSMPPVVQKPWFSKREAAVMKAW*SQKPTALATGKLRLAKRFSNWYTLKKS 201
Query: 360 VMVSLQQYLLPEHSHPLLQIFSCDFFILAFHVPAVSTSAIVPPTRIATAARASVHTMILF 181
VMV LQQYL+PEHSHPLL FSCD FHVPAV SA VPP A A +S T ILF
Sbjct: 200 VMVWLQQYLVPEHSHPLLHAFSCDSLSFTFHVPAVRISASVPPISTAAAPSSSTVTTILF 21
Query: 180 RVF 172
VF
Sbjct: 20 LVF 12
Score = 132 bits (283), Expect = 1e-030
Identities = 63/119 (52%), Positives = 70/119 (58%)
Frame = +1 / +3
Query: 184 QDHRMDAGPGCSGDSGGRHDCAGAHGGDVEGQDKEVAGEDL*QGVGVLGEQVXXXXXXXX 363
QD A G G + RH A GGDVEG+ + VAGE + QGVGVL +QV
Sbjct: 24 QDRGDGAAAGGGGGADRRHAGADPDGGDVEGEAQGVAGEGVQQGVGVLRDQVLLQPDHHR 203
Query: 364 XXXGVPVREPLXXXXXXXXXXXXVLGLPGLHHRRGPLRAPGVLHHRRQADADDGALRLP 540
GVPVREPL +LGLPGLHHRR PLR PG+LHHRR ADDGALRLP
Sbjct: 204 LLQGVPVREPLRQAQLPRRQGRRLLGLPGLHHRRLPLREPGLLHHRRHRHADDGALRLP 380
>gb|BF623654.2|BF623654 HVSMEa0003G14f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0003G14f, mRNA sequence
Length = 482
Score = 237 bits (512), Expect = 4e-062
Identities = 93/123 (75%), Positives = 100/123 (81%)
Frame = +2 / +2
Query: 173 KTRNKXXXXXXXXXXXXXXXGGTIALVLTAGTWKAKIKKSQEKICNKGWECSGSKYCCND 352
KTRNK GGT+AL+LTAGTWK K+K+S EK+CNKGWECSG+KYCCN
Sbjct: 2 KTRNKIVVTVLLLGAAAVLIGGTLALILTAGTWKVKLKESHEKVCNKGWECSGTKYCCNQ 181
Query: 353 TITDFFKVYQFENLFAKRNTPVAHAVGFWDYQAFITAAALFEPQGFCTTGGKQMQMMELC 532
TITDFFKVYQFENLFAKRN PVA AVGFWDYQAFITAA+LFE QGFCTTGG MQMMELC
Sbjct: 182 TITDFFKVYQFENLFAKRNFPVAKAVGFWDYQAFITAASLFENQGFCTTGGIDMQMMELC 361
Query: 533 AFL 541
AFL
Sbjct: 362 AFL 370
Score = 208 bits (449), Expect = 2e-053
Identities = 91/123 (73%), Positives = 95/123 (77%)
Frame = -3 / -3
Query: 540 RKAQSSIICICLPPVVQNPWGSKRAAAVMKAW*SQNPTACATGVLRLAKRFSNWYTLKKS 361
RKAQSSIIC+ +PPVVQ PW SKR AAVMKAW*SQ PTA ATG LRLAKRFSNWYTLKKS
Sbjct: 369 RKAQSSIICMSMPPVVQKPWFSKREAAVMKAW*SQKPTALATGKLRLAKRFSNWYTLKKS 190
Query: 360 VMVSLQQYLLPEHSHPLLQIFSCDFFILAFHVPAVSTSAIVPPTRIATAARASVHTMILF 181
VMV LQQYL+PEHSHPLL FSCD FHVPAV SA VPP A A +S T ILF
Sbjct: 189 VMVWLQQYLVPEHSHPLLHTFSCDSLSFTFHVPAVRISASVPPISTAAAPSSSTVTTILF 10
Query: 180 RVF 172
VF
Sbjct: 9 LVF 1
Score = 128 bits (275), Expect = 2e-029
Identities = 62/119 (52%), Positives = 69/119 (57%)
Frame = +1 / +1
Query: 184 QDHRMDAGPGCSGDSGGRHDCAGAHGGDVEGQDKEVAGEDL*QGVGVLGEQVXXXXXXXX 363
QD A G G + RH A GGDVEG+ + VA E + QGVGVL +QV
Sbjct: 13 QDRGDGAAAGGGGGADRRHAGADPDGGDVEGEAQGVAREGVQQGVGVLRDQVLLQPDHHR 192
Query: 364 XXXGVPVREPLXXXXXXXXXXXXVLGLPGLHHRRGPLRAPGVLHHRRQADADDGALRLP 540
GVPVREPL +LGLPGLHHRR PLR PG+LHHRR ADDGALRLP
Sbjct: 193 LLQGVPVREPLRQAQLPRRQGRRLLGLPGLHHRRLPLREPGLLHHRRHRHADDGALRLP 369
>gb|BF621457.2|BF621457 HVSMEa0011A19f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0011A19f, mRNA sequence
Length = 842
Score = 196 bits (422), Expect(2) = 6e-052
Identities = 76/115 (66%), Positives = 85/115 (73%)
Frame = +2 / +2
Query: 158 EKMKRKTRNKXXXXXXXXXXXXXXXGGTIALVLTAGTWKAKIKKSQEKICNKGWECSGSK 337
+KMKRKTRNK GGT+AL+LTAGTWK K+K+SQEK+CNKGWECSG+K
Sbjct: 188 KKMKRKTRNKIVVTVLLLGAAAVLIGGTLALILTAGTWKVKLKESQEKVCNKGWECSGTK 367
Query: 338 YCCNDTITDFFKVYQFENLFAKRNTPVAHAVGFWDYQAFITAAALFEPQGFCTTG 502
YCCN TITDFFKVYQFENLFAKRN PVA VG+WDYQA IT L E + F G
Sbjct: 368 YCCNQTITDFFKVYQFENLFAKRNFPVAKVVGYWDYQALITCGFLLETRAFAHRG 532
Score = 165 bits (355), Expect(2) = 3e-045
Identities = 74/106 (69%), Positives = 77/106 (72%)
Frame = -3 / -3
Query: 477 SKRAAAVMKAW*SQNPTACATGVLRLAKRFSNWYTLKKSVMVSLQQYLLPEHSHPLLQIF 298
S R V+KAW*SQ PT ATG LRLAKRFSNWYTLKKSVMV LQQYL+PEHSHPLL F
Sbjct: 507 SNRKPQVIKAW*SQ*PTTLATGKLRLAKRFSNWYTLKKSVMVWLQQYLVPEHSHPLLHTF 328
Query: 297 SCDFFILAFHVPAVSTSAIVPPTRIATAARASVHTMILFRVFRFIF 160
SCD FHVPAV SA VPP A A +S T ILF VFRFIF
Sbjct: 327 SCDSLSFTFHVPAVRISASVPPISTAAAPSSSTVTTILFLVFRFIF 190
Score = 35.9 bits (72), Expect(2) = 3e-045
Identities = 13/19 (68%), Positives = 17/19 (89%)
Frame = -3 / -2
Query: 540 RKAQSSIICICLPPVVQNP 484
RKAQSSIIC+ +PPV ++P
Sbjct: 568 RKAQSSIICMSIPPVCKSP 512
Score = 27.6 bits (54), Expect(2) = 6e-052
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +1 / +2
Query: 496 HRRQADADDGALRLP 540
HR ADDGALRLP
Sbjct: 524 HRGYRHADDGALRLP 568
>gb|BG310318.1|BG310318 HVSMEc0017K01f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0017K01f, mRNA sequence
Length = 805
Score = 164 bits (353), Expect = 3e-040
Identities = 62/100 (62%), Positives = 78/100 (78%)
Frame = +2 / +1
Query: 242 IALVLTAGTWKAKIKKSQEKICNKGWECSGSKYCCNDTITDFFKVYQFENLFAKRNTPVA 421
+ LV A+ +K E C+KGWECSGS++CCN+TITD+FK YQFE LFA+RN +A
Sbjct: 103 LLLVALLAAADARRQKGGETACDKGWECSGSRFCCNETITDYFKAYQFEELFAQRNNSLA 282
Query: 422 HAVGFWDYQAFITAAALFEPQGFCTTGGKQMQMMELCAFL 541
HA GFW+YQAFITAA+LFEP+GF TTGG++M M E+ AFL
Sbjct: 283 HAAGFWNYQAFITAASLFEPRGFGTTGGREMSMKEVAAFL 402
Score = 116 bits (247), Expect = 1e-025
Identities = 57/89 (64%), Positives = 61/89 (68%)
Frame = -3 / -3
Query: 540 RKAQSSIICICLPPVVQNPWGSKRAAAVMKAW*SQNPTACATGVLRLAKRFSNWYTLKKS 361
RKA +S + I LPPVV NP GSKR AAVMKAW* Q P A A +LR AK SNW LK S
Sbjct: 401 RKAATSFMLISLPPVVPNPRGSKRDAAVMKAW*FQKPAAWARELLRWAKSSSNW*ALK*S 222
Query: 360 VMVSLQQYLLPEHSHPLLQIFSCDFFILA 274
VMVSLQQ LLP HSHPL S F +LA
Sbjct: 221 VMVSLQQNLLPLHSHPLSHAVSPPFCLLA 135
>gb|BU983423.1|BU983423 HA29L02r HA Hordeum vulgare subsp. vulgare cDNA clone HA29L02
5-PRIME, mRNA sequence
Length = 559
Score = 164 bits (353), Expect = 3e-040
Identities = 62/100 (62%), Positives = 78/100 (78%)
Frame = +2 / +3
Query: 242 IALVLTAGTWKAKIKKSQEKICNKGWECSGSKYCCNDTITDFFKVYQFENLFAKRNTPVA 421
+ LV A+ +K E C+KGWECSGS++CCN+TITD+FK YQFE LFA+RN +A
Sbjct: 123 LLLVALLAAADARRQKGGETACDKGWECSGSRFCCNETITDYFKAYQFEELFAQRNNSLA 302
Query: 422 HAVGFWDYQAFITAAALFEPQGFCTTGGKQMQMMELCAFL 541
HA GFW+YQAFITAA+LFEP+GF TTGG++M M E+ AFL
Sbjct: 303 HAAGFWNYQAFITAASLFEPRGFGTTGGREMSMKEVAAFL 422
Score = 116 bits (247), Expect = 1e-025
Identities = 57/89 (64%), Positives = 61/89 (68%)
Frame = -3 / -1
Query: 540 RKAQSSIICICLPPVVQNPWGSKRAAAVMKAW*SQNPTACATGVLRLAKRFSNWYTLKKS 361
RKA +S + I LPPVV NP GSKR AAVMKAW* Q P A A +LR AK SNW LK S
Sbjct: 421 RKAATSFMLISLPPVVPNPRGSKRDAAVMKAW*FQKPAAWARELLRWAKSSSNW*ALK*S 242
Query: 360 VMVSLQQYLLPEHSHPLLQIFSCDFFILA 274
VMVSLQQ LLP HSHPL S F +LA
Sbjct: 241 VMVSLQQNLLPLHSHPLSHAVSPPFCLLA 155
>gb|CA029988.1|CA029988 HX05M10r HX Hordeum vulgare subsp. vulgare cDNA clone HX05M10
5-PRIME, mRNA sequence
Length = 658
Score = 164 bits (353), Expect = 3e-040
Identities = 62/100 (62%), Positives = 78/100 (78%)
Frame = +2 / +1
Query: 242 IALVLTAGTWKAKIKKSQEKICNKGWECSGSKYCCNDTITDFFKVYQFENLFAKRNTPVA 421
+ LV A+ +K E C+KGWECSGS++CCN+TITD+FK YQFE LFA+RN +A
Sbjct: 133 LLLVALLAAADARRQKGGETACDKGWECSGSRFCCNETITDYFKAYQFEELFAQRNNSLA 312
Query: 422 HAVGFWDYQAFITAAALFEPQGFCTTGGKQMQMMELCAFL 541
HA GFW+YQAFITAA+LFEP+GF TTGG++M M E+ AFL
Sbjct: 313 HAAGFWNYQAFITAASLFEPRGFGTTGGREMSMKEVAAFL 432
Score = 116 bits (247), Expect = 1e-025
Identities = 57/89 (64%), Positives = 61/89 (68%)
Frame = -3 / -3
Query: 540 RKAQSSIICICLPPVVQNPWGSKRAAAVMKAW*SQNPTACATGVLRLAKRFSNWYTLKKS 361
RKA +S + I LPPVV NP GSKR AAVMKAW* Q P A A +LR AK SNW LK S
Sbjct: 431 RKAATSFMLISLPPVVPNPRGSKRDAAVMKAW*FQKPAAWARELLRWAKSSSNW*ALK*S 252
Query: 360 VMVSLQQYLLPEHSHPLLQIFSCDFFILA 274
VMVSLQQ LLP HSHPL S F +LA
Sbjct: 251 VMVSLQQNLLPLHSHPLSHAVSPPFCLLA 165
>gb|CA031982.1|CA031982 HX11K19r HX Hordeum vulgare subsp. vulgare cDNA clone HX11K19
5-PRIME, mRNA sequence
Length = 641
Score = 164 bits (353), Expect = 3e-040
Identities = 62/100 (62%), Positives = 78/100 (78%)
Frame = +2 / +1
Query: 242 IALVLTAGTWKAKIKKSQEKICNKGWECSGSKYCCNDTITDFFKVYQFENLFAKRNTPVA 421
+ LV A+ +K E C+KGWECSGS++CCN+TITD+FK YQFE LFA+RN +A
Sbjct: 73 LLLVALLAAADARRQKGGETACDKGWECSGSRFCCNETITDYFKAYQFEELFAQRNNSLA 252
Query: 422 HAVGFWDYQAFITAAALFEPQGFCTTGGKQMQMMELCAFL 541
HA GFW+YQAFITAA+LFEP+GF TTGG++M M E+ AFL
Sbjct: 253 HAAGFWNYQAFITAASLFEPRGFGTTGGREMSMKEVAAFL 372
Score = 116 bits (247), Expect = 1e-025
Identities = 57/89 (64%), Positives = 61/89 (68%)
Frame = -3 / -1
Query: 540 RKAQSSIICICLPPVVQNPWGSKRAAAVMKAW*SQNPTACATGVLRLAKRFSNWYTLKKS 361
RKA +S + I LPPVV NP GSKR AAVMKAW* Q P A A +LR AK SNW LK S
Sbjct: 371 RKAATSFMLISLPPVVPNPRGSKRDAAVMKAW*FQKPAAWARELLRWAKSSSNW*ALK*S 192
Query: 360 VMVSLQQYLLPEHSHPLLQIFSCDFFILA 274
VMVSLQQ LLP HSHPL S F +LA
Sbjct: 191 VMVSLQQNLLPLHSHPLSHAVSPPFCLLA 105
>gb|CK122262.1|CK122262 BES1824102j21 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010J212 5-PRIME, mRNA sequence
Length = 803
Score = 164 bits (353), Expect = 3e-040
Identities = 62/100 (62%), Positives = 78/100 (78%)
Frame = +2 / +3
Query: 242 IALVLTAGTWKAKIKKSQEKICNKGWECSGSKYCCNDTITDFFKVYQFENLFAKRNTPVA 421
+ LV A+ +K E C+KGWECSGS++CCN+TITD+FK YQFE LFA+RN +A
Sbjct: 117 LLLVALLAAADARRQKGGETACDKGWECSGSRFCCNETITDYFKAYQFEELFAQRNNSLA 296
Query: 422 HAVGFWDYQAFITAAALFEPQGFCTTGGKQMQMMELCAFL 541
HA GFW+YQAFITAA+LFEP+GF TTGG++M M E+ AFL
Sbjct: 297 HAAGFWNYQAFITAASLFEPRGFGTTGGREMSMKEVAAFL 416
Score = 116 bits (247), Expect = 1e-025
Identities = 57/89 (64%), Positives = 61/89 (68%)
Frame = -3 / -2
Query: 540 RKAQSSIICICLPPVVQNPWGSKRAAAVMKAW*SQNPTACATGVLRLAKRFSNWYTLKKS 361
RKA +S + I LPPVV NP GSKR AAVMKAW* Q P A A +LR AK SNW LK S
Sbjct: 415 RKAATSFMLISLPPVVPNPRGSKRDAAVMKAW*FQKPAAWARELLRWAKSSSNW*ALK*S 236
Query: 360 VMVSLQQYLLPEHSHPLLQIFSCDFFILA 274
VMVSLQQ LLP HSHPL S F +LA
Sbjct: 235 VMVSLQQNLLPLHSHPLSHAVSPPFCLLA 149
>gb|BQ765072.1|BQ765072 EBca01_SQ005_P15_R carpel, pre-anthesis, no treatment, cv Optic,
EBca01 Hordeum vulgare subsp. vulgare cDNA clone
EBca01_SQ005_P15 5', mRNA sequence
Length = 655
Score = 131 bits (280), Expect(2) = 5e-035
Identities = 49/82 (59%), Positives = 62/82 (75%)
Frame = +2 / +1
Query: 242 IALVLTAGTWKAKIKKSQEKICNKGWECSGSKYCCNDTITDFFKVYQFENLFAKRNTPVA 421
+ LV A+ +K E C+KGWECSGS++CCN+TITD+FK YQFE LFA+RN +A
Sbjct: 154 LLLVALLAAADARRQKGGETACDKGWECSGSRFCCNETITDYFKAYQFEELFAQRNNSLA 333
Query: 422 HAVGFWDYQAFITAAALFEPQG 487
HA GFW+YQAFITAA+LF+ G
Sbjct: 334 HAAGFWNYQAFITAASLFDLAG 399
Score = 88.6 bits (187), Expect(2) = 4e-021
Identities = 45/71 (63%), Positives = 47/71 (66%)
Frame = -3 / -3
Query: 486 PWGSKRAAAVMKAW*SQNPTACATGVLRLAKRFSNWYTLKKSVMVSLQQYLLPEHSHPLL 307
P SKR AAVMKAW* Q P A A +LR AK SNW LK SVMVSLQQ LLP HSHPL
Sbjct: 398 PARSKRDAAVMKAW*FQKPAAWARELLRWAKSSSNW*ALK*SVMVSLQQNLLPLHSHPLS 219
Query: 306 QIFSCDFFILA 274
S F +LA
Sbjct: 218 HAVSPPFCLLA 186
Score = 35.9 bits (72), Expect(2) = 5e-035
Identities = 13/21 (61%), Positives = 17/21 (80%)
Frame = +2 / +3
Query: 479 PQGFCTTGGKQMQMMELCAFL 541
P+GF TTGG++M M E+ AFL
Sbjct: 390 PRGFGTTGGREMSMKEVAAFL 452
Score = 31.8 bits (63), Expect(2) = 4e-021
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = -3 / -1
Query: 540 RKAQSSIICICLPPVVQNPWGSK 472
RKA +S + I LPPVV NP G +
Sbjct: 451 RKAATSFMLISLPPVVPNPRGRR 383
>gb|AW982890.1|AW982890 HVSMEg0004H18f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0004H18f, mRNA sequence
Length = 955
Score = 131 bits (280), Expect = 4e-030
Identities = 50/87 (57%), Positives = 62/87 (71%)
Frame = +2 / +1
Query: 242 IALVLTAGTWKAKIKKSQEKICNKGWECSGSKYCCNDTITDFFKVYQFENLFAKRNTPVA 421
+ LV A+ +K E C+KGWEC+ S++CCN+TITD+FK YQFE LF +RN +A
Sbjct: 61 LLLVALLAAADARRQKGGETACDKGWECTRSRFCCNETITDYFKAYQFEELFFQRNNFLA 240
Query: 422 HAVGFWDYQAFITAAALFEPQGFCTTG 502
HA GFW YQAFIT LFEP+GF TTG
Sbjct: 241 HAAGFWKYQAFITDECLFEPRGFGTTG 321
>gb|BI958161.1|BI958161 HVSMEn0013L01f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0013L01f, mRNA sequence
Length = 933
Score = 112 bits (240), Expect = 1e-024
Identities = 46/52 (88%), Positives = 47/52 (90%)
Frame = +2 / +1
Query: 386 ENLFAKRNTPVAHAVGFWDYQAFITAAALFEPQGFCTTGGKQMQMMELCAFL 541
ENLFAKRN PVA AVGFWDYQAFITAA+LFE QGFCTTGG MQMMELCAFL
Sbjct: 61 ENLFAKRNFPVAKAVGFWDYQAFITAASLFENQGFCTTGGIDMQMMELCAFL 216
Score = 100 bits (214), Expect(2) = 2e-028
Identities = 44/59 (74%), Positives = 48/59 (81%)
Frame = -3 / -2
Query: 540 RKAQSSIICICLPPVVQNPWGSKRAAAVMKAW*SQNPTACATGVLRLAKRFSNWYTLKK 364
RKAQSSIIC+ +PPVVQ PW SKR AAVMKAW*SQ PTA ATG LRLAKRFS L++
Sbjct: 215 RKAQSSIICMSMPPVVQKPWFSKREAAVMKAW*SQKPTALATGKLRLAKRFSQLVHLEE 39
Score = 44.1 bits (90), Expect(2) = 2e-028
Identities = 17/19 (89%), Positives = 18/19 (94%)
Frame = -3 / -3
Query: 384 NWYTLKKSVMVSLQQYLLP 328
NWYTLKKSVMV LQQYL+P
Sbjct: 58 NWYTLKKSVMVWLQQYLVP 2
>gb|BE215083.3|BE215083 HV_CEb0005M01f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0005M01f, mRNA sequence
Length = 616
Score = 106 bits (227), Expect = 7e-023
Identities = 50/109 (45%), Positives = 59/109 (54%)
Frame = +2 / +2
Query: 158 EKMKRKTRNKXXXXXXXXXXXXXXXGGTIALVLTAGTWKAKIKKSQEKICNKGWECSGSK 337
+KMKRKTRNK GGT+AL+LTAGTWK K+K+S EK+CNKG +
Sbjct: 290 KKMKRKTRNKIVVTVLLLGAAAVLIGGTLALILTAGTWKVKLKESHEKVCNKGVGVPRDQ 469
Query: 338 YCCNDTITDFFKVYQFENLFAKRNTPVAHAVGFWDYQAFITAAALFEPQ 484
+ TIT + Q ENLF KRN PV A IT LFE Q
Sbjct: 470 VLVHQTITGLLQGDQXENLFGKRNFPVGKGRRXXGLXALITGRFLFEDQ 616
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 177,643,787
Number of Sequences: 312970
Number of extensions: 2386381
Number of successful extensions: 65458
Number of sequences better than 1.0e-020: 17
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 5554858270
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)