TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4574602.2.1
(1248 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF262553.2|BF262553 HV_CEa0004F01f Hordeum vulgare seedl... 155 3e-078
gb|BI952990.1|BI952990 HVSMEm0008P06f Hordeum vulgare green... 155 6e-076
gb|BQ762605.1|BQ762605 EBro02_SQ004_N09 _R root, 3 week, hy... 155 9e-074
gb|BQ762734.1|BQ762734 EBro02_SQ004_L21_R root, 3 week, hyd... 155 2e-069
gb|CB877860.1|CB877860 HP06H01T HP Hordeum vulgare subsp. v... 155 8e-064
gb|BQ764132.1|BQ764132 EBro03_SQ006_I12_R root, 3 week, wat... 153 1e-063
gb|CB878248.1|CB878248 HP07N14T HP Hordeum vulgare subsp. v... 153 4e-063
gb|BF267787.2|BF267787 HV_CEa0018O07f Hordeum vulgare seedl... 130 7e-057
gb|BM371207.2|BM371207 EBro04_SQ003_N15_R root, 3 week, sal... 153 2e-054
gb|BQ768001.1|BQ768001 EBro08_SQ010_M09_R root, 3 week, dro... 111 6e-049
gb|AL450587.1|AL450587 AL450587 Hordeum vulgare Barke etiol... 115 5e-043
gb|CB879431.1|CB879431 HP02D14w HP Hordeum vulgare subsp. v... 83 9e-043
gb|CB876733.1|CB876733 HP02D14T HP Hordeum vulgare subsp. v... 154 1e-040
gb|BG368670.1|BG368670 HVSMEi0020C19f Hordeum vulgare 20 DA... 61 2e-038
gb|BG369379.2|BG369379 HVSMEi0024C18f Hordeum vulgare 20 DA... 61 1e-037
gb|BI952762.1|BI952762 HVSMEm0007K18f Hordeum vulgare green... 108 3e-035
gb|CB877554.1|CB877554 HP05F18T HP Hordeum vulgare subsp. v... 59 1e-032
gb|AL505584.1|AL505584 AL505584 Hordeum vulgare Barke roots... 61 6e-030
gb|AL504101.1|AL504101 AL504101 Hordeum vulgare Barke roots... 51 1e-029
gb|BG365477.2|BG365477 HVSMEi0002N15f Hordeum vulgare 20 DA... 49 5e-028
gb|BQ471515.1|BQ471515 HV02K17r HV Hordeum vulgare subsp. v... 49 5e-026
gb|BM443361.2|BM443361 EBro02_SQ003_M08_R root, 3 week, hyd... 111 6e-026
gb|BF257596.2|BF257596 HVSMEf0013E23f Hordeum vulgare seedl... 61 6e-026
gb|BM371947.2|BM371947 EBma08_SQ004_H18_R maternal, 28 DPA,... 61 2e-025
gb|CA018087.1|CA018087 HV07G18r HV Hordeum vulgare subsp. v... 61 3e-025
gb|BF256681.2|BF256681 HVSMEf0010L04f Hordeum vulgare seedl... 58 4e-024
gb|AL508981.1|AL508981 AL508981 Hordeum vulgare Barke devel... 61 5e-024
gb|BF255528.3|BF255528 HVSMEf0007B16f Hordeum vulgare seedl... 49 8e-024
gb|CK122763.1|CK122763 BES1824105l04 BES1824 Hordeum vulgar... 59 9e-024
gb|BI780772.2|BI780772 EBes01_SQ002_C13_R embryo sac, 4-6 D... 59 9e-024
gb|BF624578.2|BF624578 HVSMEa0017C20f Hordeum vulgare seedl... 62 4e-023
gb|BQ740058.1|BQ740058 HC04B02 HC Hordeum vulgare subsp. vu... 61 4e-023
gb|BG300616.1|BG300616 HVSMEb0017L09f Hordeum vulgare seedl... 59 2e-022
gb|CK125329.1|CK125329 BES1824107l08 BES1824 Hordeum vulgar... 60 2e-022
gb|BF259054.2|BF259054 HVSMEf0017I14f Hordeum vulgare seedl... 62 6e-022
gb|BF625166.2|BF625166 HVSMEa0006B24f Hordeum vulgare seedl... 62 6e-022
gb|BQ758987.1|BQ758987 EBma07_SQ003_N09_R maternal, 21 DPA,... 53 9e-021
>gb|BF262553.2|BF262553 HV_CEa0004F01f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0004F01f, mRNA sequence
Length = 843
Score = 155 bits (334), Expect(4) = 3e-078
Identities = 63/89 (70%), Positives = 74/89 (83%)
Frame = -2 / +1
Query: 923 SGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGSGSSRP 744
SGSCT +D+++CQS GIKVLLSIGG GSYGLSS DDA VA YLW+N+LGG+ +SRP
Sbjct: 253 SGSCTFVGADVKSCQSRGIKVLLSIGGGVGSYGLSSADDAKEVAKYLWENYLGGTSASRP 432
Query: 743 LGAAVLDGIDFDIENGQSAHYDDLANALK 657
LG AVLDG+DFDIE+G SAH+DDLA LK
Sbjct: 433 LGDAVLDGVDFDIESGGSAHWDDLARELK 519
Score = 110 bits (235), Expect(4) = 3e-078
Identities = 44/82 (53%), Positives = 54/82 (65%)
Frame = -2 / +1
Query: 1166 SNMAANLKWAPXXXXXXXXXAMVGTTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAF 987
+ MA+ L A + G AG IA+YWGQNGNEG+LA AC +G Y +VN+AF
Sbjct: 7 TKMASKLFPAMLVRAAVTMAGLAGGPRAGGIAIYWGQNGNEGTLAQACATGNYKFVNVAF 186
Query: 986 LTTFGNGQTPVLNLAGHCDPGS 921
L TFG GQ P+LNLAGHCDP +
Sbjct: 187 LFTFGRGQKPLLNLAGHCDPAT 252
Score = 104 bits (221), Expect(4) = 3e-045
Identities = 50/75 (66%), Positives = 53/75 (70%)
Frame = +1 / -2
Query: 661 SAFARSS*CADWPFSMSKSMPSSTAAPRGRDEPLPPRKLSHR*SATLLASSVEERP*LPL 840
S+ ARSS CA P SMSKS PSSTA+PRG D +PPR S R AT LASS EERP*LP
Sbjct: 515 SSRARSSQCALPPLSMSKSTPSSTASPRGLDADVPPR*FSQRYLATSLASSAEERP*LPT 336
Query: 841 APPMERSTLMPSDWQ 885
PPME TLMP WQ
Sbjct: 335 PPPMESRTLMPRLWQ 291
Score = 66.6 bits (139), Expect(4) = 3e-045
Identities = 32/53 (60%), Positives = 34/53 (64%)
Frame = +1 / -2
Query: 928 GSQCPARLRTGVCPLPKVVRNAMLT*A*SPELQASARLPSLPFWPQYTAMLPA 1086
GSQ PAR +G PLPKV R A LT * P A A +PSLPFWPQ AM PA
Sbjct: 245 GSQWPARFSSGFWPLPKVKRKATLTNL*FPVAHA*ASVPSLPFWPQ*IAMPPA 87
Score = 48.3 bits (99), Expect(4) = 3e-078
Identities = 17/25 (68%), Positives = 19/25 (76%)
Frame = -2 / +1
Query: 599 LGPALQTGQFDNVWIQFYNNPGCAY 525
LG AL+TG D VW+QFYNNP C Y
Sbjct: 607 LGGALRTGLLDYVWVQFYNNPACHY 681
Score = 39.1 bits (79), Expect(4) = 3e-045
Identities = 18/26 (69%), Positives = 18/26 (69%)
Frame = +1 / -2
Query: 532 HPGLL*NCIHTLSNWPVCSAGPSDAS 609
H GLL*NC HT SN PV SA PS S
Sbjct: 674 HAGLL*NCTHT*SNSPVRSAPPSPPS 597
Score = 35.9 bits (72), Expect(4) = 3e-078
Identities = 13/17 (76%), Positives = 14/17 (82%)
Frame = -2 / +1
Query: 644 VLLTAAPQCPYPDASLG 594
V L AAPQCP+PDA LG
Sbjct: 553 VYLAAAPQCPFPDAMLG 603
Score = 29.0 bits (57), Expect(4) = 3e-045
Identities = 12/17 (70%), Positives = 12/17 (70%)
Frame = +1 / -2
Query: 595 PSDASG*GHCGAAVSST 645
PS ASG GHCGAA T
Sbjct: 602 PSIASGNGHCGAAARYT 552
>gb|BI952990.1|BI952990 HVSMEm0008P06f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0008P06f, mRNA sequence
Length = 787
Score = 155 bits (334), Expect(4) = 6e-076
Identities = 63/89 (70%), Positives = 74/89 (83%)
Frame = -2 / +2
Query: 923 SGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGSGSSRP 744
SGSCT +D+++CQS GIKVLLSIGG GSYGLSS DDA VA YLW+N+LGG+ +SRP
Sbjct: 260 SGSCTFVGADVKSCQSRGIKVLLSIGGGVGSYGLSSADDAKEVAKYLWENYLGGTSASRP 439
Query: 743 LGAAVLDGIDFDIENGQSAHYDDLANALK 657
LG AVLDG+DFDIE+G SAH+DDLA LK
Sbjct: 440 LGDAVLDGVDFDIESGGSAHWDDLARELK 526
Score = 108 bits (230), Expect(4) = 6e-076
Identities = 40/61 (65%), Positives = 48/61 (78%)
Frame = -2 / +2
Query: 1103 MVGTTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNGQTPVLNLAGHCDPG 924
+ G AG IA+YWGQNGNEG+LA AC +G Y +VN+AFL TFG GQ P+LNLAGHCDP
Sbjct: 77 LAGGARAGGIAIYWGQNGNEGTLAXACATGDYKFVNVAFLFTFGRGQKPLLNLAGHCDPA 256
Query: 923 S 921
+
Sbjct: 257 T 259
Score = 105 bits (225), Expect(4) = 5e-046
Identities = 51/76 (67%), Positives = 54/76 (71%)
Frame = +1 / -2
Query: 658 LSAFARSS*CADWPFSMSKSMPSSTAAPRGRDEPLPPRKLSHR*SATLLASSVEERP*LP 837
LS+ ARSS CA P SMSKS PSSTA+PRG D +PPR S R AT LASS EERP*LP
Sbjct: 525 LSSRARSSQCALPPLSMSKSTPSSTASPRGLDADVPPR*FSQRYLATSLASSAEERP*LP 346
Query: 838 LAPPMERSTLMPSDWQ 885
PPME TLMP WQ
Sbjct: 345 TPPPMESRTLMPRLWQ 298
Score = 70.7 bits (148), Expect(4) = 5e-046
Identities = 34/57 (59%), Positives = 36/57 (63%)
Frame = +1 / -2
Query: 928 GSQCPARLRTGVCPLPKVVRNAMLT*A*SPELQASARLPSLPFWPQYTAMLPALVVP 1098
GSQ PAR +G PLPKV R A LT *SP A A +PSLPFWPQ AM PA P
Sbjct: 252 GSQWPARFSSGFWPLPKVKRKATLTNL*SPVAHA*ASVPSLPFWPQ*IAMPPARAPP 82
Score = 42.8 bits (87), Expect(4) = 6e-076
Identities = 16/25 (64%), Positives = 17/25 (68%)
Frame = -2 / +2
Query: 599 LGPALQTGQFDNVWIQFYNNPGCAY 525
LG AL TG VW+QFYNNP C Y
Sbjct: 614 LGGALGTGLVHYVWVQFYNNPPCQY 688
Score = 35.9 bits (72), Expect(4) = 6e-076
Identities = 13/17 (76%), Positives = 14/17 (82%)
Frame = -2 / +2
Query: 644 VLLTAAPQCPYPDASLG 594
V L AAPQCP+PDA LG
Sbjct: 560 VYLAAAPQCPFPDAMLG 610
Score = 35.9 bits (72), Expect(4) = 5e-046
Identities = 18/30 (60%), Positives = 18/30 (60%)
Frame = +1 / -2
Query: 520 LAYAHPGLL*NCIHTLSNWPVCSAGPSDAS 609
L Y H GLL*NC HT PV SA PS S
Sbjct: 693 LLYWHGGLL*NCTHT*WTSPVPSAPPSPPS 604
Score = 29.0 bits (57), Expect(4) = 5e-046
Identities = 12/17 (70%), Positives = 12/17 (70%)
Frame = +1 / -2
Query: 595 PSDASG*GHCGAAVSST 645
PS ASG GHCGAA T
Sbjct: 609 PSIASGNGHCGAAARYT 559
>gb|BQ762605.1|BQ762605 EBro02_SQ004_N09 _R root, 3 week, hydroponic grown, low nitrogen,
cv Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA
clone EBro02_SQ004_N09 5', mRNA sequence
Length = 670
Score = 155 bits (334), Expect(4) = 9e-074
Identities = 63/89 (70%), Positives = 74/89 (83%)
Frame = -2 / +2
Query: 923 SGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGSGSSRP 744
SGSCT +D+++CQS GIKVLLSIGG GSYGLSS DDA VA YLW+N+LGG+ +SRP
Sbjct: 266 SGSCTFVGADVKSCQSRGIKVLLSIGGGVGSYGLSSADDAKEVAKYLWENYLGGTSASRP 445
Query: 743 LGAAVLDGIDFDIENGQSAHYDDLANALK 657
LG AVLDG+DFDIE+G SAH+DDLA LK
Sbjct: 446 LGDAVLDGVDFDIESGGSAHWDDLARELK 532
Score = 115 bits (245), Expect(4) = 9e-074
Identities = 46/85 (54%), Positives = 56/85 (65%)
Frame = -2 / +2
Query: 1175 RPVSNMAANLKWAPXXXXXXXXXAMVGTTSAGNIAVYWGQNGNEGSLADACNSGLYAYVN 996
RP + MA+ L A + G AG IA+YWGQNGNEG+LA AC +G Y +VN
Sbjct: 11 RPGTKMASKLFPAMLLLAAVTMAGLAGGARAGGIAIYWGQNGNEGTLAQACATGNYKFVN 190
Query: 995 IAFLTTFGNGQTPVLNLAGHCDPGS 921
+AFL TFG GQ P+LNLAGHCDP +
Sbjct: 191 VAFLFTFGRGQKPLLNLAGHCDPAT 265
Score = 105 bits (225), Expect(3) = 7e-040
Identities = 51/76 (67%), Positives = 54/76 (71%)
Frame = +1 / -2
Query: 658 LSAFARSS*CADWPFSMSKSMPSSTAAPRGRDEPLPPRKLSHR*SATLLASSVEERP*LP 837
LS+ ARSS CA P SMSKS PSSTA+PRG D +PPR S R AT LASS EERP*LP
Sbjct: 531 LSSRARSSQCALPPLSMSKSTPSSTASPRGLDADVPPR*FSQRYLATSLASSAEERP*LP 352
Query: 838 LAPPMERSTLMPSDWQ 885
PPME TLMP WQ
Sbjct: 351 TPPPMESRTLMPRLWQ 304
Score = 68.0 bits (142), Expect(3) = 7e-040
Identities = 33/57 (57%), Positives = 35/57 (61%)
Frame = +1 / -2
Query: 928 GSQCPARLRTGVCPLPKVVRNAMLT*A*SPELQASARLPSLPFWPQYTAMLPALVVP 1098
GSQ PAR +G PLPKV R A LT * P A A +PSLPFWPQ AM PA P
Sbjct: 258 GSQWPARFSSGFWPLPKVKRKATLTNL*FPVAHA*ASVPSLPFWPQ*IAMPPARAPP 88
Score = 35.9 bits (72), Expect(4) = 9e-074
Identities = 13/17 (76%), Positives = 14/17 (82%)
Frame = -2 / +2
Query: 644 VLLTAAPQCPYPDASLG 594
V L AAPQCP+PDA LG
Sbjct: 566 VYLAAAPQCPFPDAMLG 616
Score = 29.0 bits (57), Expect(3) = 7e-040
Identities = 12/17 (70%), Positives = 12/17 (70%)
Frame = +1 / -2
Query: 595 PSDASG*GHCGAAVSST 645
PS ASG GHCGAA T
Sbjct: 615 PSIASGNGHCGAAARYT 565
Score = 28.6 bits (56), Expect(4) = 9e-074
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = -2 / +2
Query: 599 LGPALQTGQFDNVWIQF 549
LG AL TG D VW+QF
Sbjct: 620 LGGALGTGLLDYVWVQF 670
>gb|BQ762734.1|BQ762734 EBro02_SQ004_L21_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ004_L21 5', mRNA sequence
Length = 629
Score = 155 bits (334), Expect(3) = 2e-069
Identities = 63/89 (70%), Positives = 74/89 (83%)
Frame = -2 / +1
Query: 923 SGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGSGSSRP 744
SGSCT +D+++CQS GIKVLLSIGG GSYGLSS DDA VA YLW+N+LGG+ +SRP
Sbjct: 262 SGSCTFVGADVKSCQSRGIKVLLSIGGGVGSYGLSSADDAKEVAKYLWENYLGGTSASRP 441
Query: 743 LGAAVLDGIDFDIENGQSAHYDDLANALK 657
LG AVLDG+DFDIE+G SAH+DDLA LK
Sbjct: 442 LGDAVLDGVDFDIESGGSAHWDDLARELK 528
Score = 111 bits (236), Expect(3) = 2e-069
Identities = 44/82 (53%), Positives = 54/82 (65%)
Frame = -2 / +1
Query: 1166 SNMAANLKWAPXXXXXXXXXAMVGTTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAF 987
+ MA+ L A + G AG IA+YWGQNGNEG+LA AC +G Y +VN+AF
Sbjct: 16 TKMASKLFPAMLLLAAVTMAGLAGGARAGGIAIYWGQNGNEGTLAQACATGNYKFVNVAF 195
Query: 986 LTTFGNGQTPVLNLAGHCDPGS 921
L TFG GQ P+LNLAGHCDP +
Sbjct: 196 LFTFGRGQKPLLNLAGHCDPAT 261
Score = 105 bits (225), Expect(3) = 7e-040
Identities = 51/76 (67%), Positives = 54/76 (71%)
Frame = +1 / -1
Query: 658 LSAFARSS*CADWPFSMSKSMPSSTAAPRGRDEPLPPRKLSHR*SATLLASSVEERP*LP 837
LS+ ARSS CA P SMSKS PSSTA+PRG D +PPR S R AT LASS EERP*LP
Sbjct: 527 LSSRARSSQCALPPLSMSKSTPSSTASPRGLDADVPPR*FSQRYLATSLASSAEERP*LP 348
Query: 838 LAPPMERSTLMPSDWQ 885
PPME TLMP WQ
Sbjct: 347 TPPPMESRTLMPRLWQ 300
Score = 68.0 bits (142), Expect(3) = 7e-040
Identities = 33/57 (57%), Positives = 35/57 (61%)
Frame = +1 / -1
Query: 928 GSQCPARLRTGVCPLPKVVRNAMLT*A*SPELQASARLPSLPFWPQYTAMLPALVVP 1098
GSQ PAR +G PLPKV R A LT * P A A +PSLPFWPQ AM PA P
Sbjct: 254 GSQWPARFSSGFWPLPKVKRKATLTNL*FPVAHA*ASVPSLPFWPQ*IAMPPARAPP 84
Score = 35.9 bits (72), Expect(3) = 2e-069
Identities = 13/17 (76%), Positives = 14/17 (82%)
Frame = -2 / +1
Query: 644 VLLTAAPQCPYPDASLG 594
V L AAPQCP+PDA LG
Sbjct: 562 VYLAAAPQCPFPDAMLG 612
Score = 29.0 bits (57), Expect(3) = 7e-040
Identities = 12/17 (70%), Positives = 12/17 (70%)
Frame = +1 / -1
Query: 595 PSDASG*GHCGAAVSST 645
PS ASG GHCGAA T
Sbjct: 611 PSIASGNGHCGAAARYT 561
>gb|CB877860.1|CB877860 HP06H01T HP Hordeum vulgare subsp. vulgare cDNA clone HP06H01
5-PRIME, mRNA sequence
Length = 509
Score = 155 bits (334), Expect(2) = 8e-064
Identities = 63/89 (70%), Positives = 74/89 (83%)
Frame = -2 / +3
Query: 923 SGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGSGSSRP 744
SGSCT +D+++CQS GIKVLLSIGG GSYGLSS DDA VA YLW+N+LGG+ +SRP
Sbjct: 243 SGSCTFVGADVKSCQSRGIKVLLSIGGGVGSYGLSSADDAKEVAKYLWENYLGGTSASRP 422
Query: 743 LGAAVLDGIDFDIENGQSAHYDDLANALK 657
LG AVLDG+DFDIE+G SAH+DDLA LK
Sbjct: 423 LGDAVLDGVDFDIESGGSAHWDDLARELK 509
Score = 109 bits (232), Expect(2) = 8e-064
Identities = 40/61 (65%), Positives = 48/61 (78%)
Frame = -2 / +3
Query: 1103 MVGTTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNGQTPVLNLAGHCDPG 924
+ G AG IA+YWGQNGNEG+LA AC +G Y +VN+AFL TFG GQ P+LNLAGHCDP
Sbjct: 60 LAGGARAGGIAIYWGQNGNEGTLAQACATGNYKFVNVAFLFTFGRGQKPLLNLAGHCDPA 239
Query: 923 S 921
+
Sbjct: 240 T 242
Score = 105 bits (225), Expect(2) = 1e-036
Identities = 51/76 (67%), Positives = 54/76 (71%)
Frame = +1 / -2
Query: 658 LSAFARSS*CADWPFSMSKSMPSSTAAPRGRDEPLPPRKLSHR*SATLLASSVEERP*LP 837
LS+ ARSS CA P SMSKS PSSTA+PRG D +PPR S R AT LASS EERP*LP
Sbjct: 508 LSSRARSSQCALPPLSMSKSTPSSTASPRGLDADVPPR*FSQRYLATSLASSAEERP*LP 329
Query: 838 LAPPMERSTLMPSDWQ 885
PPME TLMP WQ
Sbjct: 328 TPPPMESRTLMPRLWQ 281
Score = 68.0 bits (142), Expect(2) = 1e-036
Identities = 33/57 (57%), Positives = 35/57 (61%)
Frame = +1 / -2
Query: 928 GSQCPARLRTGVCPLPKVVRNAMLT*A*SPELQASARLPSLPFWPQYTAMLPALVVP 1098
GSQ PAR +G PLPKV R A LT * P A A +PSLPFWPQ AM PA P
Sbjct: 235 GSQWPARFSSGFWPLPKVKRKATLTNL*FPVAHA*ASVPSLPFWPQ*IAMPPARAPP 65
>gb|BQ764132.1|BQ764132 EBro03_SQ006_I12_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ006_I12 5', mRNA sequence
Length = 537
Score = 153 bits (328), Expect(2) = 1e-063
Identities = 62/89 (69%), Positives = 73/89 (82%)
Frame = -2 / +1
Query: 923 SGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGSGSSRP 744
SGSCT +D+++CQS GIKVLLSIGG GSYGLSS DDA VA YLW+N+LGG+ +SRP
Sbjct: 259 SGSCTFVGADVKSCQSRGIKVLLSIGGGVGSYGLSSADDAKEVAKYLWENYLGGTSASRP 438
Query: 743 LGAAVLDGIDFDIENGQSAHYDDLANALK 657
LG AVLDG+DFDI +G SAH+DDLA LK
Sbjct: 439 LGDAVLDGVDFDIXSGGSAHWDDLARELK 525
Score = 111 bits (236), Expect(2) = 1e-063
Identities = 44/82 (53%), Positives = 54/82 (65%)
Frame = -2 / +1
Query: 1166 SNMAANLKWAPXXXXXXXXXAMVGTTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAF 987
+ MA+ L A + G AG IA+YWGQNGNEG+LA AC +G Y +VN+AF
Sbjct: 13 TKMASKLFPAMLLLAAVTMAGLAGGARAGGIAIYWGQNGNEGTLAQACATGNYKFVNVAF 192
Query: 986 LTTFGNGQTPVLNLAGHCDPGS 921
L TFG GQ P+LNLAGHCDP +
Sbjct: 193 LFTFGRGQKPLLNLAGHCDPAT 258
Score = 104 bits (221), Expect(2) = 5e-036
Identities = 50/76 (65%), Positives = 53/76 (69%)
Frame = +1 / -2
Query: 658 LSAFARSS*CADWPFSMSKSMPSSTAAPRGRDEPLPPRKLSHR*SATLLASSVEERP*LP 837
LS+ ARSS CA P MSKS PSSTA+PRG D +PPR S R AT LASS EERP*LP
Sbjct: 524 LSSRARSSQCALPPLXMSKSTPSSTASPRGLDADVPPR*FSQRYLATSLASSAEERP*LP 345
Query: 838 LAPPMERSTLMPSDWQ 885
PPME TLMP WQ
Sbjct: 344 TPPPMESRTLMPRLWQ 297
Score = 68.0 bits (142), Expect(2) = 5e-036
Identities = 33/57 (57%), Positives = 35/57 (61%)
Frame = +1 / -2
Query: 928 GSQCPARLRTGVCPLPKVVRNAMLT*A*SPELQASARLPSLPFWPQYTAMLPALVVP 1098
GSQ PAR +G PLPKV R A LT * P A A +PSLPFWPQ AM PA P
Sbjct: 251 GSQWPARFSSGFWPLPKVKRKATLTNL*FPVAHA*ASVPSLPFWPQ*IAMPPARAPP 81
>gb|CB878248.1|CB878248 HP07N14T HP Hordeum vulgare subsp. vulgare cDNA clone HP07N14
5-PRIME, mRNA sequence
Length = 506
Score = 153 bits (329), Expect(2) = 4e-063
Identities = 62/88 (70%), Positives = 73/88 (82%)
Frame = -2 / +3
Query: 923 SGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGSGSSRP 744
SGSCT +D+++CQS GIKVLLSIGG GSYGLSS DDA VA YLW+N+LGG+ +SRP
Sbjct: 243 SGSCTFVGADVKSCQSRGIKVLLSIGGGVGSYGLSSADDAKEVAKYLWENYLGGTSASRP 422
Query: 743 LGAAVLDGIDFDIENGQSAHYDDLANAL 660
LG AVLDG+DFDIE+G SAH+DDLA L
Sbjct: 423 LGDAVLDGVDFDIESGGSAHWDDLAREL 506
Score = 109 bits (232), Expect(2) = 4e-063
Identities = 40/61 (65%), Positives = 48/61 (78%)
Frame = -2 / +3
Query: 1103 MVGTTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNGQTPVLNLAGHCDPG 924
+ G AG IA+YWGQNGNEG+LA AC +G Y +VN+AFL TFG GQ P+LNLAGHCDP
Sbjct: 60 LAGGARAGGIAIYWGQNGNEGTLAQACATGNYKFVNVAFLFTFGRGQKPLLNLAGHCDPA 239
Query: 923 S 921
+
Sbjct: 240 T 242
Score = 104 bits (221), Expect(2) = 5e-036
Identities = 50/75 (66%), Positives = 53/75 (70%)
Frame = +1 / -2
Query: 661 SAFARSS*CADWPFSMSKSMPSSTAAPRGRDEPLPPRKLSHR*SATLLASSVEERP*LPL 840
S+ ARSS CA P SMSKS PSSTA+PRG D +PPR S R AT LASS EERP*LP
Sbjct: 505 SSRARSSQCALPPLSMSKSTPSSTASPRGLDADVPPR*FSQRYLATSLASSAEERP*LPT 326
Query: 841 APPMERSTLMPSDWQ 885
PPME TLMP WQ
Sbjct: 325 PPPMESRTLMPRLWQ 281
Score = 68.0 bits (142), Expect(2) = 5e-036
Identities = 33/57 (57%), Positives = 35/57 (61%)
Frame = +1 / -2
Query: 928 GSQCPARLRTGVCPLPKVVRNAMLT*A*SPELQASARLPSLPFWPQYTAMLPALVVP 1098
GSQ PAR +G PLPKV R A LT * P A A +PSLPFWPQ AM PA P
Sbjct: 235 GSQWPARFSSGFWPLPKVKRKATLTNL*FPVAHA*ASVPSLPFWPQ*IAMPPARAPP 65
>gb|BF267787.2|BF267787 HV_CEa0018O07f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0018O07f, mRNA sequence
Length = 828
Score = 130 bits (279), Expect(2) = 7e-057
Identities = 54/88 (61%), Positives = 67/88 (76%)
Frame = -2 / +3
Query: 923 SGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGSGSSRP 744
SGSCT +D+++CQS GIKVLLSIGG GSYGLSS DD VA YLW+N+LGG+ ++RP
Sbjct: 264 SGSCTFVGADVKSCQSRGIKVLLSIGGGVGSYGLSSADDTKEVAKYLWENYLGGTTATRP 443
Query: 743 LGAAVLDGIDFDIENGQSAHYDDLANAL 660
LG A+LDG D D+ +G SAH D+L L
Sbjct: 444 LGDAMLDGRDCDMNSGGSAH*DNLTRDL 527
Score = 111 bits (236), Expect(2) = 7e-057
Identities = 44/82 (53%), Positives = 54/82 (65%)
Frame = -2 / +3
Query: 1166 SNMAANLKWAPXXXXXXXXXAMVGTTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAF 987
+ MA+ L A + G AG IA+YWGQNGNEG+LA AC +G Y +VN+AF
Sbjct: 18 TKMASKLFPAMLLLAAVTMAGLAGGARAGGIAIYWGQNGNEGTLAQACATGNYKFVNVAF 197
Query: 986 LTTFGNGQTPVLNLAGHCDPGS 921
L TFG GQ P+LNLAGHCDP +
Sbjct: 198 LFTFGRGQKPLLNLAGHCDPAT 263
Score = 88.6 bits (187), Expect(2) = 2e-031
Identities = 43/71 (60%), Positives = 46/71 (64%)
Frame = +1 / -3
Query: 673 RSS*CADWPFSMSKSMPSSTAAPRGRDEPLPPRKLSHR*SATLLASSVEERP*LPLAPPM 852
R S CA P MS+S PSS A+PRG +PPR SHR AT L SS EERP*LP PPM
Sbjct: 514 RLSQCALPPLFMSQSRPSSMASPRGLVAVVPPR*FSHRYLATSLVSSAEERP*LPTPPPM 335
Query: 853 ERSTLMPSDWQ 885
E TLMP WQ
Sbjct: 334 ESRTLMPRLWQ 302
Score = 68.0 bits (142), Expect(2) = 2e-031
Identities = 33/57 (57%), Positives = 35/57 (61%)
Frame = +1 / -3
Query: 928 GSQCPARLRTGVCPLPKVVRNAMLT*A*SPELQASARLPSLPFWPQYTAMLPALVVP 1098
GSQ PAR +G PLPKV R A LT * P A A +PSLPFWPQ AM PA P
Sbjct: 256 GSQWPARFSSGFWPLPKVKRKATLTNL*FPVAHA*ASVPSLPFWPQ*IAMPPARAPP 86
>gb|BM371207.2|BM371207 EBro04_SQ003_N15_R root, 3 week, salt-stressed, cv Optic, EBro04
Hordeum vulgare subsp. vulgare cDNA clone
EBro04_SQ003_N15 5', mRNA sequence
Length = 579
Score = 153 bits (328), Expect(4) = 2e-054
Identities = 62/89 (69%), Positives = 74/89 (83%)
Frame = -2 / +1
Query: 923 SGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGSGSSRP 744
SGSCT +D+++CQS GIKVLLSIGG + SYGLSS DDA VA YLW+N+LGG+ +SRP
Sbjct: 55 SGSCTFVGADVKSCQSRGIKVLLSIGGGACSYGLSSADDAKEVAKYLWENYLGGTSASRP 234
Query: 743 LGAAVLDGIDFDIENGQSAHYDDLANALK 657
LG AVLDG+DFDIE+G SAH+DDLA LK
Sbjct: 235 LGDAVLDGVDFDIESGGSAHWDDLARELK 321
Score = 102 bits (217), Expect(3) = 5e-031
Identities = 50/76 (65%), Positives = 53/76 (69%)
Frame = +1 / -2
Query: 658 LSAFARSS*CADWPFSMSKSMPSSTAAPRGRDEPLPPRKLSHR*SATLLASSVEERP*LP 837
LS+ ARSS CA P SMSKS PSSTA+PRG D +PPR S R AT LASS EERP*L
Sbjct: 320 LSSRARSSQCALPPLSMSKSTPSSTASPRGLDADVPPR*FSQRYLATSLASSAEERP*LQ 141
Query: 838 LAPPMERSTLMPSDWQ 885
PPME TLMP WQ
Sbjct: 140 APPPMESRTLMPRLWQ 93
Score = 49.2 bits (101), Expect(4) = 2e-054
Identities = 18/25 (72%), Positives = 19/25 (76%)
Frame = -2 / +1
Query: 599 LGPALQTGQFDNVWIQFYNNPGCAY 525
LG AL TG FD VW+QFYNNP C Y
Sbjct: 409 LGGALGTGLFDYVWVQFYNNPPCQY 483
Score = 41.8 bits (85), Expect(3) = 5e-031
Identities = 20/30 (66%), Positives = 20/30 (66%)
Frame = +1 / -2
Query: 520 LAYAHPGLL*NCIHTLSNWPVCSAGPSDAS 609
L Y H GLL*NC HT SN PV SA PS S
Sbjct: 488 LVYWHGGLL*NCTHT*SNNPVPSAPPSPPS 399
Score = 35.9 bits (72), Expect(4) = 2e-054
Identities = 13/17 (76%), Positives = 14/17 (82%)
Frame = -2 / +1
Query: 644 VLLTAAPQCPYPDASLG 594
V L AAPQCP+PDA LG
Sbjct: 355 VYLAAAPQCPFPDAMLG 405
Score = 31.8 bits (63), Expect(4) = 2e-054
Identities = 10/13 (76%), Positives = 12/13 (92%)
Frame = -2 / +1
Query: 959 PVLNLAGHCDPGS 921
P+LNLAGHCDP +
Sbjct: 16 PLLNLAGHCDPAT 54
Score = 29.0 bits (57), Expect(3) = 5e-031
Identities = 12/17 (70%), Positives = 12/17 (70%)
Frame = +1 / -2
Query: 595 PSDASG*GHCGAAVSST 645
PS ASG GHCGAA T
Sbjct: 404 PSIASGNGHCGAAARYT 354
>gb|BQ768001.1|BQ768001 EBro08_SQ010_M09_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ010_M09 5', mRNA sequence
Length = 440
Score = 111 bits (236), Expect(2) = 6e-049
Identities = 44/82 (53%), Positives = 54/82 (65%)
Frame = -2 / +1
Query: 1166 SNMAANLKWAPXXXXXXXXXAMVGTTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAF 987
+ MA+ L A + G AG IA+YWGQNGNEG+LA AC +G Y +VN+AF
Sbjct: 13 TKMASKLFPAMLLLAAVTMAGLAGGARAGGIAIYWGQNGNEGTLAQACATGNYKFVNVAF 192
Query: 986 LTTFGNGQTPVLNLAGHCDPGS 921
L TFG GQ P+LNLAGHCDP +
Sbjct: 193 LFTFGRGQKPLLNLAGHCDPAT 258
Score = 104 bits (221), Expect(2) = 6e-049
Identities = 41/60 (68%), Positives = 49/60 (81%)
Frame = -2 / +1
Query: 923 SGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGSGSSRP 744
SGSCT +D+++CQS GIKVLLSIGG GSYGLSS DDA VA YLW+N+LGG+ +SRP
Sbjct: 259 SGSCTFVGADVKSCQSRGIKVLLSIGGGVGSYGLSSADDAKEVAKYLWENYLGGTSASRP 438
Score = 68.0 bits (142), Expect(2) = 4e-025
Identities = 33/57 (57%), Positives = 35/57 (61%)
Frame = +1 / -1
Query: 928 GSQCPARLRTGVCPLPKVVRNAMLT*A*SPELQASARLPSLPFWPQYTAMLPALVVP 1098
GSQ PAR +G PLPKV R A LT * P A A +PSLPFWPQ AM PA P
Sbjct: 251 GSQWPARFSSGFWPLPKVKRKATLTNL*FPVAHA*ASVPSLPFWPQ*IAMPPARAPP 81
Score = 67.5 bits (141), Expect(2) = 4e-025
Identities = 31/48 (64%), Positives = 32/48 (66%)
Frame = +1 / -1
Query: 742 RGRDEPLPPRKLSHR*SATLLASSVEERP*LPLAPPMERSTLMPSDWQ 885
RG D +PPR S R AT LASS EERP*LP PPME TLMP WQ
Sbjct: 440 RGLDADVPPR*FSQRYLATSLASSAEERP*LPTPPPMESRTLMPRLWQ 297
>gb|AL450587.1|AL450587 AL450587 Hordeum vulgare Barke etiolated leaves Hordeum vulgare
subsp. vulgare cDNA clone HK03E19u 5', mRNA sequence
Length = 511
Score = 115 bits (246), Expect(2) = 5e-043
Identities = 45/64 (70%), Positives = 51/64 (79%)
Frame = -2 / +2
Query: 464 AGSFYLGVPASPQAAGSGYIDPGTLTGTVIPAIRGIGNYGGIMVWDRFNDVQNNYSSQVK 285
+G FYLGVPAS AAGSGY+ PG LT V+P + G NYGGIMVWDR+NDVQN+YS QVK
Sbjct: 119 SGGFYLGVPASTAAAGSGYVSPGDLTSAVLPGVTGASNYGGIMVWDRYNDVQNSYSEQVK 298
Query: 284 GSV* 273
SV*
Sbjct: 299 DSV* 310
Score = 79.9 bits (168), Expect(2) = 5e-043
Identities = 27/37 (72%), Positives = 32/37 (86%)
Frame = -2 / +2
Query: 578 GQFDNVWIQFYNNPGCAYANGDDTNLVNAWNTWTSSI 468
G FDNVW+QFYNNP C YA+GD +NL +AWNTWTSS+
Sbjct: 2 GLFDNVWVQFYNNPPCQYASGDASNLQSAWNTWTSSV 112
Score = 67.5 bits (141), Expect(3) = 4e-025
Identities = 28/36 (77%), Positives = 30/36 (83%)
Frame = +1 / -2
Query: 472 LLVHVFHAFTRLVSSPLAYAHPGLL*NCIHTLSNWP 579
LLVHVFHA RL++SPLAY H GLL*NC HTLSN P
Sbjct: 108 LLVHVFHALCRLLASPLAYWHGGLL*NCTHTLSNSP 1
Score = 48.3 bits (99), Expect(3) = 4e-025
Identities = 22/36 (61%), Positives = 23/36 (63%)
Frame = +1 / -2
Query: 373 GITVPVRVPGSMYPLPAAWGDAGTPR*KLPAVMLLV 480
G T VR PG YPLPAA DAGTPR*K P L+
Sbjct: 210 GSTADVRSPGDTYPLPAAAVDAGTPR*KPPDTFTLL 103
Score = 37.3 bits (75), Expect(3) = 4e-025
Identities = 17/21 (80%), Positives = 17/21 (80%)
Frame = +1 / -2
Query: 274 QTLPFTWLL*LFCTSLKRSHT 336
QTL FT L*LFCTSL RSHT
Sbjct: 309 QTLSFTCSL*LFCTSL*RSHT 247
>gb|CB879431.1|CB879431 HP02D14w HP Hordeum vulgare subsp. vulgare cDNA clone HP02D14
3-PRIME, mRNA sequence
Length = 665
Score = 82.6 bits (174), Expect(4) = 9e-043
Identities = 33/68 (48%), Positives = 46/68 (67%)
Frame = -2 / -3
Query: 476 SSITAGSFYLGVPASPQAAGSGYIDPGTLTGTVIPAIRGIGNYGGIMVWDRFNDVQNNYS 297
+SI A +LG+PA+P AAGSG+++P L V+P ++ YGGIM+W RF D Q +S
Sbjct: 348 ASIPARQVFLGLPAAPAAAGSGFVEPSDLLSEVLPVVQKSPKYGGIMLWSRFFDGQTGFS 169
Query: 296 SQVKGSV* 273
+VK SV*
Sbjct: 168 DKVKSSV* 145
Score = 62.9 bits (131), Expect(4) = 9e-043
Identities = 25/35 (71%), Positives = 30/35 (85%)
Frame = -2 / -3
Query: 761 SGSSRPLGAAVLDGIDFDIENGQSAHYDDLANALK 657
+ +SRPLG AVLDG+DFDIE+G SAH+DDLA LK
Sbjct: 663 TSASRPLGDAVLDGVDFDIESGGSAHWDDLARELK 559
Score = 49.2 bits (101), Expect(4) = 9e-043
Identities = 18/25 (72%), Positives = 19/25 (76%)
Frame = -2 / -3
Query: 599 LGPALQTGQFDNVWIQFYNNPGCAY 525
LG AL TG FD VW+QFYNNP C Y
Sbjct: 471 LGGALGTGLFDYVWVQFYNNPPCQY 397
Score = 35.9 bits (72), Expect(4) = 9e-043
Identities = 13/17 (76%), Positives = 14/17 (82%)
Frame = -2 / -3
Query: 644 VLLTAAPQCPYPDASLG 594
V L AAPQCP+PDA LG
Sbjct: 525 VYLAAAPQCPFPDAMLG 475
>gb|CB876733.1|CB876733 HP02D14T HP Hordeum vulgare subsp. vulgare cDNA clone HP02D14
5-PRIME, mRNA sequence
Length = 340
Score = 154 bits (331), Expect(2) = 1e-040
Identities = 62/89 (69%), Positives = 74/89 (83%)
Frame = -2 / +2
Query: 923 SGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGSGSSRP 744
SGSCT +D+++CQS GIKVLLSIGG GSYGLSS DDA VA YLW+N+LGG+ +SRP
Sbjct: 62 SGSCTFVGADVKSCQSRGIKVLLSIGGGVGSYGLSSADDAKEVAKYLWENYLGGTSASRP 241
Query: 743 LGAAVLDGIDFDIENGQSAHYDDLANALK 657
LG AVLDG+DFDIE+G SAH+DDL+ LK
Sbjct: 242 LGDAVLDGVDFDIESGGSAHWDDLSRELK 328
Score = 103 bits (219), Expect = 3e-021
Identities = 50/76 (65%), Positives = 53/76 (69%)
Frame = +1 / -2
Query: 658 LSAFARSS*CADWPFSMSKSMPSSTAAPRGRDEPLPPRKLSHR*SATLLASSVEERP*LP 837
LS+ RSS CA P SMSKS PSSTA+PRG D +PPR S R AT LASS EERP*LP
Sbjct: 327 LSSRDRSSQCALPPLSMSKSTPSSTASPRGLDADVPPR*FSQRYLATSLASSAEERP*LP 148
Query: 838 LAPPMERSTLMPSDWQ 885
PPME TLMP WQ
Sbjct: 147 TPPPMESRTLMPRLWQ 100
Score = 32.7 bits (65), Expect(2) = 1e-040
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = -2 / +2
Query: 968 GQTPVLNLAGHCDPGS 921
G P+LNLAGHCDP +
Sbjct: 14 GCRPLLNLAGHCDPAT 61
>gb|BG368670.1|BG368670 HVSMEi0020C19f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0020C19f, mRNA sequence
Length = 830
Score = 60.6 bits (126), Expect(5) = 2e-038
Identities = 22/43 (51%), Positives = 29/43 (67%)
Frame = -2 / +1
Query: 1094 TTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNG 966
T G +AV+WG+N NEGSL +AC++G Y I+FL FG G
Sbjct: 100 TGKTGQVAVFWGRNKNEGSLREACDTGTYTIAIISFLDVFGRG 228
Score = 50.6 bits (104), Expect(5) = 2e-038
Identities = 23/60 (38%), Positives = 31/60 (51%)
Frame = -2 / +1
Query: 938 HCDPGSGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGS 759
H D + +DI+ CQS I V LSIGG Y L + A VA YLW+ ++ G+
Sbjct: 235 HLDLSGHDVSAVGADIKHCQSKNILVFLSIGGFGKQYSLPTAHSAADVAYYLWNAYMLGT 414
Score = 48.7 bits (100), Expect(5) = 2e-038
Identities = 18/44 (40%), Positives = 28/44 (63%)
Frame = -2 / +1
Query: 659 KGKGSVLLTAAPQCPYPDASLGPALQTGQFDNVWIQFYNNPGCA 528
+G+ V LTA P+C YPD + AL TG ++++FY++ CA
Sbjct: 541 RGRTPVQLTATPRCGYPDRHVERALATGLVGRIFVRFYDDADCA 672
Score = 43.7 bits (89), Expect(5) = 2e-038
Identities = 19/33 (57%), Positives = 21/33 (63%)
Frame = -2 / +1
Query: 758 GSSRPLGAAVLDGIDFDIENGQSAHYDDLANAL 660
G RP G A DGIDF IENG +YD+LA L
Sbjct: 421 GVYRPFGDAFTDGIDFFIENGAPDNYDELAKRL 519
Score = 29.5 bits (58), Expect(5) = 2e-038
Identities = 11/35 (31%), Positives = 15/35 (42%)
Frame = -2 / +3
Query: 506 NLVNAWNTWTSSITAGSFYLGVPASPQAAGSGYID 402
N W+ WT++ YLG+ Q G G D
Sbjct: 675 NWQRQWDMWTAAYPRAQIYLGLAGLKQKCGDGSKD 779
>gb|BG369379.2|BG369379 HVSMEi0024C18f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0024C18f, mRNA sequence
Length = 859
Score = 60.6 bits (126), Expect(5) = 1e-037
Identities = 22/43 (51%), Positives = 29/43 (67%)
Frame = -2 / +2
Query: 1094 TTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNG 966
T G +AV+WG+N NEGSL +AC++G Y I+FL FG G
Sbjct: 95 TGKTGQVAVFWGRNKNEGSLREACDTGTYTIAIISFLDVFGRG 223
Score = 50.6 bits (104), Expect(5) = 1e-037
Identities = 23/60 (38%), Positives = 31/60 (51%)
Frame = -2 / +2
Query: 938 HCDPGSGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGS 759
H D + +DI+ CQS I V LSIGG Y L + A VA YLW+ ++ G+
Sbjct: 230 HLDLSGHDVSAVGADIKHCQSKNILVFLSIGGFGKQYSLPTAHSAADVAYYLWNAYMLGT 409
Score = 47.8 bits (98), Expect(5) = 1e-037
Identities = 18/44 (40%), Positives = 28/44 (63%)
Frame = -2 / +2
Query: 659 KGKGSVLLTAAPQCPYPDASLGPALQTGQFDNVWIQFYNNPGCA 528
+G+ V LTA P+C YPD + AL TG ++++FY++ CA
Sbjct: 536 RGRTPVQLTATPRCGYPDRHVERALATGLEGRIFVRFYDDADCA 667
Score = 44.6 bits (91), Expect(5) = 1e-037
Identities = 19/33 (57%), Positives = 22/33 (66%)
Frame = -2 / +2
Query: 758 GSSRPLGAAVLDGIDFDIENGQSAHYDDLANAL 660
G RP G A +DGIDF IENG +YD+LA L
Sbjct: 416 GVYRPFGDAFVDGIDFFIENGAPDNYDELAKRL 514
Score = 26.7 bits (52), Expect(5) = 1e-037
Identities = 10/31 (32%), Positives = 15/31 (48%)
Frame = -2 / +3
Query: 506 NLVNAWNTWTSSITAGSFYLGVPASPQAAGS 414
N W+ WT+ + YLG P S + + S
Sbjct: 672 NWQRQWDKWTAVCSIAQIYLGWPRSKKRSES 764
>gb|BI952762.1|BI952762 HVSMEm0007K18f Hordeum vulgare green seedling EST library HVcDNA0014
(Blumeria infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEm0007K18f, mRNA sequence
Length = 418
Score = 108 bits (230), Expect(4) = 3e-035
Identities = 39/64 (60%), Positives = 47/64 (73%)
Frame = -2 / +1
Query: 1103 MVGTTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNGQTPVLNLAGHCDPG 924
+ G AG I +YWGQNGNEG+LA AC +G Y +VN+AFL TFG G P+LNLAGHCDP
Sbjct: 43 LAGGARAGGITIYWGQNGNEGTLAQACATGNYKFVNVAFLFTFGRGHKPLLNLAGHCDPA 222
Query: 923 SGSC 912
+ C
Sbjct: 223 APQC 234
Score = 45.5 bits (93), Expect(4) = 3e-035
Identities = 17/25 (68%), Positives = 18/25 (72%)
Frame = -2 / +1
Query: 599 LGPALQTGQFDNVWIQFYNNPGCAY 525
LG AL TG F VW+QFYNNP C Y
Sbjct: 262 LGGALGTGLFHYVWVQFYNNPPCHY 336
Score = 32.7 bits (65), Expect(4) = 3e-035
Identities = 11/13 (84%), Positives = 12/13 (92%)
Frame = -2 / +1
Query: 632 AAPQCPYPDASLG 594
AAPQCP+PDA LG
Sbjct: 220 AAPQCPFPDAMLG 258
Score = 21.7 bits (41), Expect(4) = 3e-035
Identities = 7/15 (46%), Positives = 8/15 (53%)
Frame = -2 / +1
Query: 518 GDDTNLVNAWNTWTS 474
G NL +AW W S
Sbjct: 346 GGAGNLASAWQKWAS 390
>gb|CB877554.1|CB877554 HP05F18T HP Hordeum vulgare subsp. vulgare cDNA clone HP05F18
5-PRIME, mRNA sequence
Length = 590
Score = 58.8 bits (122), Expect(4) = 1e-032
Identities = 21/39 (53%), Positives = 28/39 (71%)
Frame = -2 / +3
Query: 1082 GNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNG 966
G +AV+WG+N NEGSL +AC++G Y I+FL FG G
Sbjct: 30 GQLAVFWGRNKNEGSLREACDTGTYTIAIISFLDVFGRG 146
Score = 48.7 bits (100), Expect(4) = 1e-032
Identities = 22/60 (36%), Positives = 30/60 (50%)
Frame = -2 / +3
Query: 938 HCDPGSGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGS 759
H D + +DI+ CQS I V LSIGG Y L + VA YLW+ ++ G+
Sbjct: 153 HLDLSGHDVSAVGADIKHCQSKNILVFLSIGGFGKQYSLPTAHSVADVAYYLWNAYMLGT 332
Score = 44.6 bits (91), Expect(4) = 1e-032
Identities = 19/33 (57%), Positives = 22/33 (66%)
Frame = -2 / +3
Query: 758 GSSRPLGAAVLDGIDFDIENGQSAHYDDLANAL 660
G RP G A +DGIDF IENG +YD+LA L
Sbjct: 339 GVYRPFGDAFVDGIDFFIENGAPDNYDELAKRL 437
Score = 44.1 bits (90), Expect(4) = 1e-032
Identities = 17/44 (38%), Positives = 27/44 (61%)
Frame = -2 / +3
Query: 659 KGKGSVLLTAAPQCPYPDASLGPALQTGQFDNVWIQFYNNPGCA 528
+G+ V LTA +C YPD + AL TG ++++FY++ CA
Sbjct: 459 RGRTPVQLTATLRCGYPDRHVERALATGLVGRIFVRFYDDADCA 590
>gb|AL505584.1|AL505584 AL505584 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW08O17V 5', mRNA sequence
Length = 691
Score = 60.6 bits (126), Expect(4) = 6e-030
Identities = 22/43 (51%), Positives = 29/43 (67%)
Frame = -2 / +3
Query: 1094 TTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNG 966
T G +AV+WG+N NEGSL +AC++G Y I+FL FG G
Sbjct: 111 TGKTGQVAVFWGRNKNEGSLREACDTGTYTIAIISFLDVFGRG 239
Score = 50.6 bits (104), Expect(4) = 6e-030
Identities = 23/60 (38%), Positives = 31/60 (51%)
Frame = -2 / +3
Query: 938 HCDPGSGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGS 759
H D + +DI+ CQS I V LSIGG Y L + A VA YLW+ ++ G+
Sbjct: 246 HLDLSGHDVSAVGADIKHCQSKNILVFLSIGGFGKQYSLPTAHSAADVAYYLWNAYMLGT 425
Score = 44.6 bits (91), Expect(4) = 6e-030
Identities = 19/33 (57%), Positives = 22/33 (66%)
Frame = -2 / +3
Query: 758 GSSRPLGAAVLDGIDFDIENGQSAHYDDLANAL 660
G RP G A +DGIDF IENG +YD+LA L
Sbjct: 432 GVYRPFGDAFVDGIDFFIENGAPDNYDELAKRL 530
Score = 30.9 bits (61), Expect(4) = 6e-030
Identities = 16/29 (55%), Positives = 16/29 (55%)
Frame = -1 / +2
Query: 654 QGERAADGGAAVPLPGRVAGAGAADGPVR 568
Q AA G AAV LPG GAGA G R
Sbjct: 557 QDAGAAHGHAAVRLPGPARGAGARHGARR 643
>gb|AL504101.1|AL504101 AL504101 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW04D09V 5', mRNA sequence
Length = 700
Score = 50.6 bits (104), Expect(5) = 1e-029
Identities = 23/60 (38%), Positives = 31/60 (51%)
Frame = -2 / +3
Query: 938 HCDPGSGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGS 759
H D + +DI+ CQS I V LSIGG Y L + A VA YLW+ ++ G+
Sbjct: 117 HLDLSGHDVSAVGADIKHCQSKNILVFLSIGGFGKQYSLPTAHSAADVAYYLWNAYMLGT 296
Score = 44.6 bits (91), Expect(5) = 1e-029
Identities = 19/33 (57%), Positives = 22/33 (66%)
Frame = -2 / +3
Query: 758 GSSRPLGAAVLDGIDFDIENGQSAHYDDLANAL 660
G RP G A +DGIDF IENG +YD+LA L
Sbjct: 303 GVYRPFGDAFVDGIDFFIENGAPDNYDELAKRL 401
Score = 42.3 bits (86), Expect(5) = 1e-029
Identities = 16/30 (53%), Positives = 20/30 (66%)
Frame = -2 / +3
Query: 1055 NGNEGSLADACNSGLYAYVNIAFLTTFGNG 966
N NEGSL +AC++G Y I+FL FG G
Sbjct: 21 NXNEGSLREACDTGTYTIAIISFLDVFGRG 110
Score = 40.0 bits (81), Expect(5) = 1e-029
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = -2 / +3
Query: 659 KGKGSVLLTAAPQCPYPDASLGPALQTGQFDNVWIQFYNNPGCA 528
+G+ LT +C YPD + AL TG ++++FY++ CA
Sbjct: 423 RGRTPXQLTXTLRCGYPDRHVERALATGLVGRIFVRFYDDADCA 554
Score = 24.9 bits (48), Expect(5) = 1e-029
Identities = 9/25 (36%), Positives = 14/25 (56%)
Frame = -2 / +3
Query: 506 NLVNAWNTWTSSITAGSFYLGVPAS 432
N W+ T++ + YLG+PAS
Sbjct: 558 NWQRQWDKXTAAYPSXQIYLGLPAS 632
>gb|BG365477.2|BG365477 HVSMEi0002N15f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0002N15f, mRNA sequence
Length = 859
Score = 48.7 bits (100), Expect(5) = 5e-028
Identities = 18/44 (40%), Positives = 28/44 (63%)
Frame = -2 / +1
Query: 659 KGKGSVLLTAAPQCPYPDASLGPALQTGQFDNVWIQFYNNPGCA 528
+G+ V LTA P+C YPD + AL TG ++++FY++ CA
Sbjct: 244 RGRTPVQLTATPRCGYPDRHVERALATGLVGRIFVRFYDDADCA 375
Score = 44.1 bits (90), Expect(5) = 5e-028
Identities = 18/33 (54%), Positives = 22/33 (66%)
Frame = -2 / +1
Query: 758 GSSRPLGAAVLDGIDFDIENGQSAHYDDLANAL 660
G RP G A +DG+DF IENG +YD+LA L
Sbjct: 124 GVYRPFGDAFVDGVDFFIENGAPDNYDELAKRL 222
Score = 35.9 bits (72), Expect(5) = 5e-028
Identities = 16/37 (43%), Positives = 21/37 (56%)
Frame = -2 / +1
Query: 869 IKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGS 759
I V LSIGG Y L + A VA YLW+ ++ G+
Sbjct: 7 ILVFLSIGGFGKQYSLPTAHSAADVAYYLWNAYMLGT 117
Score = 35.4 bits (71), Expect(5) = 5e-028
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -2 / +1
Query: 506 NLVNAWNTWTSSITAGSFYLGVPASPQAAG 417
N W+ WT++ + YLG+PAS Q G
Sbjct: 379 NWQRQWDKWTAAYPSAQIYLGLPASEQKVG 468
Score = 32.7 bits (65), Expect(5) = 5e-028
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = -2 / +1
Query: 413 GYIDPGTLTGTVIPAIRGIGNYGGIMVWD 327
GY+ P L VI ++ NYGG M WD
Sbjct: 466 GYVHPKNLYYGVIQVVQKAANYGGGMGWD 552
>gb|BQ471515.1|BQ471515 HV02K17r HV Hordeum vulgare subsp. vulgare cDNA clone HV02K17
5-PRIME, mRNA sequence
Length = 573
Score = 49.2 bits (101), Expect(4) = 5e-026
Identities = 22/56 (39%), Positives = 31/56 (55%)
Frame = -2 / +2
Query: 926 GSGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGS 759
G+ + +DI+ CQS I V LSIGG Y L + A VA YLW+ ++ G+
Sbjct: 2 GTRDVSAVGADIKHCQSKNILVFLSIGGFGKQYSLPTAHSAADVAYYLWNAYMLGT 169
Score = 44.6 bits (91), Expect(4) = 5e-026
Identities = 19/33 (57%), Positives = 22/33 (66%)
Frame = -2 / +2
Query: 758 GSSRPLGAAVLDGIDFDIENGQSAHYDDLANAL 660
G RP G A +DGIDF IENG +YD+LA L
Sbjct: 176 GVYRPFGDAFVDGIDFFIENGAPDNYDELAKRL 274
Score = 44.1 bits (90), Expect(4) = 5e-026
Identities = 17/44 (38%), Positives = 27/44 (61%)
Frame = -2 / +2
Query: 659 KGKGSVLLTAAPQCPYPDASLGPALQTGQFDNVWIQFYNNPGCA 528
+G+ V LTA +C YPD + AL TG ++++FY++ CA
Sbjct: 296 RGRTPVQLTATLRCGYPDRHVERALATGLVGRIFVRFYDDADCA 427
Score = 35.4 bits (71), Expect(4) = 5e-026
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -2 / +2
Query: 506 NLVNAWNTWTSSITAGSFYLGVPASPQAAG 417
N W+ WT++ + YLG+PAS Q G
Sbjct: 431 NWQRQWDKWTAAYPSAQIYLGLPASEQKVG 520
>gb|BM443361.2|BM443361 EBro02_SQ003_M08_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ003_M08 5', mRNA sequence
Length = 311
Score = 111 bits (236), Expect(2) = 6e-026
Identities = 44/82 (53%), Positives = 54/82 (65%)
Frame = -2 / +1
Query: 1166 SNMAANLKWAPXXXXXXXXXAMVGTTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAF 987
+ MA+ L A + G AG IA+YWGQNGNEG+LA AC +G Y +VN+AF
Sbjct: 16 TKMASKLFPAMLLLAAVTMAGLAGGARAGGIAIYWGQNGNEGTLAQACATGNYKFVNVAF 195
Query: 986 LTTFGNGQTPVLNLAGHCDPGS 921
L TFG GQ P+LNLAGHCDP +
Sbjct: 196 LFTFGRGQKPLLNLAGHCDPAT 261
Score = 27.2 bits (53), Expect(2) = 6e-026
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = -2 / +1
Query: 923 SGSCTGQSSDIQTCQS 876
SGSCT +D+++CQS
Sbjct: 262 SGSCTFVGADVKSCQS 309
>gb|BF257596.2|BF257596 HVSMEf0013E23f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEf0013E23f, mRNA sequence
Length = 528
Score = 60.6 bits (126), Expect(3) = 6e-026
Identities = 22/43 (51%), Positives = 29/43 (67%)
Frame = -2 / +1
Query: 1094 TTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNG 966
T G +AV+WG+N NEGSL +AC++G Y I+FL FG G
Sbjct: 85 TGKTGQVAVFWGRNKNEGSLREACDTGTYTIAIISFLDVFGRG 213
Score = 50.6 bits (104), Expect(3) = 6e-026
Identities = 23/60 (38%), Positives = 31/60 (51%)
Frame = -2 / +1
Query: 938 HCDPGSGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGS 759
H D + +DI+ CQS I V LSIGG Y L + A VA YLW+ ++ G+
Sbjct: 220 HLDLSGHDVSAVGADIKHCQSKNILVFLSIGGFGKQYSLPTAHSAADVAYYLWNAYMLGT 399
Score = 44.6 bits (91), Expect(3) = 6e-026
Identities = 19/33 (57%), Positives = 22/33 (66%)
Frame = -2 / +1
Query: 758 GSSRPLGAAVLDGIDFDIENGQSAHYDDLANAL 660
G RP G A +DGIDF IENG +YD+LA L
Sbjct: 406 GVYRPFGDAFVDGIDFFIENGAPDNYDELAKRL 504
>gb|BM371947.2|BM371947 EBma08_SQ004_H18_R maternal, 28 DPA, no treatment, cv Optic, EBma08
Hordeum vulgare subsp. vulgare cDNA clone
EBma08_SQ004_H18 5', mRNA sequence
Length = 514
Score = 60.6 bits (126), Expect(3) = 2e-025
Identities = 22/43 (51%), Positives = 29/43 (67%)
Frame = -2 / +3
Query: 1094 TTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNG 966
T G +AV+WG+N NEGSL +AC++G Y I+FL FG G
Sbjct: 102 TGKTGQVAVFWGRNKNEGSLREACDTGTYTIAIISFLDVFGRG 230
Score = 50.6 bits (104), Expect(3) = 2e-025
Identities = 23/60 (38%), Positives = 31/60 (51%)
Frame = -2 / +3
Query: 938 HCDPGSGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGS 759
H D + +DI+ CQS I V LSIGG Y L + A VA YLW+ ++ G+
Sbjct: 237 HLDLSGHDVSAVGADIKHCQSKNILVFLSIGGFGKQYSLPTAHSAADVAYYLWNAYMLGT 416
Score = 43.2 bits (88), Expect(3) = 2e-025
Identities = 18/30 (60%), Positives = 21/30 (70%)
Frame = -2 / +3
Query: 758 GSSRPLGAAVLDGIDFDIENGQSAHYDDLA 669
G RP G A +DGIDF IENG +YD+LA
Sbjct: 423 GVYRPFGDAFVDGIDFFIENGAPDNYDELA 512
>gb|CA018087.1|CA018087 HV07G18r HV Hordeum vulgare subsp. vulgare cDNA clone HV07G18
5-PRIME, mRNA sequence
Length = 554
Score = 60.6 bits (126), Expect(3) = 3e-025
Identities = 22/43 (51%), Positives = 29/43 (67%)
Frame = -2 / +3
Query: 1094 TTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNG 966
T G +AV+WG+N NEGSL +AC++G Y I+FL FG G
Sbjct: 90 TGKTGQVAVFWGRNKNEGSLREACDTGTYTIAIISFLDVFGRG 218
Score = 50.6 bits (104), Expect(3) = 3e-025
Identities = 23/60 (38%), Positives = 31/60 (51%)
Frame = -2 / +3
Query: 938 HCDPGSGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGS 759
H D + +DI+ CQS I V LSIGG Y L + A VA YLW+ ++ G+
Sbjct: 225 HLDLSGHDVSAVGADIKHCQSKNILVFLSIGGFGKQYSLPTAHSAADVAYYLWNAYMLGT 404
Score = 42.3 bits (86), Expect(3) = 3e-025
Identities = 18/33 (54%), Positives = 21/33 (63%)
Frame = -2 / +3
Query: 758 GSSRPLGAAVLDGIDFDIENGQSAHYDDLANAL 660
G RP G A +DGIDF IENG +YD+L L
Sbjct: 411 GVYRPFGDAFVDGIDFFIENGAPDNYDELXXRL 509
>gb|BF256681.2|BF256681 HVSMEf0010L04f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEf0010L04f, mRNA sequence
Length = 842
Score = 58.3 bits (121), Expect(4) = 4e-024
Identities = 22/49 (44%), Positives = 32/49 (65%)
Frame = -2 / +2
Query: 1082 GNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNGQTPVLNLAGH 936
G + V+WG+N EGSL +AC+SG+Y ++FL FG L+L+GH
Sbjct: 122 GQVTVFWGRNKAEGSLREACDSGMYTMGTMSFLDVFGANGQYHLDLSGH 268
Score = 55.1 bits (114), Expect(4) = 4e-024
Identities = 24/65 (36%), Positives = 33/65 (50%)
Frame = -2 / +2
Query: 938 HCDPGSGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGS 759
H D + +DI+ CQ +G+ V LSIGG Y L S A + D+LW+ + GGS
Sbjct: 248 HLDLSGHDLSSVGADIKHCQFMGVPVSLSIGGYGTGYSLPSNRSALDLFDHLWNYYFGGS 427
Query: 758 GSSRP 744
P
Sbjct: 428 KPGVP 442
Score = 29.9 bits (59), Expect(4) = 4e-024
Identities = 9/24 (37%), Positives = 15/24 (62%)
Frame = -2 / +2
Query: 494 AWNTWTSSITAGSFYLGVPASPQA 423
+WN WT++ A FY+G+ Q+
Sbjct: 716 SWNEWTAAYPATRFYVGLTGDEQS 787
Score = 29.9 bits (59), Expect(4) = 4e-024
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = -2 / +2
Query: 758 GSSRPLGAAVLDGIDFDIENGQSAHYDDL 672
G RP G A LDG+D +E+G A D+
Sbjct: 434 GVPRPFGDAWLDGVDLFLEHGTPADRYDV 520
>gb|AL508981.1|AL508981 AL508981 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY10F19V 5',
mRNA sequence
Length = 589
Score = 60.6 bits (126), Expect(3) = 5e-024
Identities = 24/49 (48%), Positives = 31/49 (63%)
Frame = -2 / +3
Query: 908 GQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGG 762
G DI+ CQS G+ V LSIGG G Y L + A +AD+LW ++LGG
Sbjct: 261 GIGDDIRHCQSAGVTVSLSIGGFGGDYALPTNQSALDLADHLWXSYLGG 407
Score = 56.5 bits (117), Expect(3) = 5e-024
Identities = 19/44 (43%), Positives = 31/44 (70%)
Frame = -2 / +3
Query: 1094 TTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNGQ 963
T G + V+WG+N +EGSL +AC++G+Y V + FL +G+G+
Sbjct: 96 TGKTGQLTVFWGRNKDEGSLREACDAGVYTAVIMXFLNVYGHGK 227
Score = 32.2 bits (64), Expect(3) = 5e-024
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = -2 / +3
Query: 758 GSSRPLGAAVLDGIDFDIENGQSAHYDD 675
G RP G A LDG+DF +E G + D
Sbjct: 417 GVRRPFGRARLDGVDFFLERGGPGEHYD 500
>gb|BF255528.3|BF255528 HVSMEf0007B16f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0007B16f, mRNA sequence
Length = 853
Score = 48.7 bits (100), Expect(4) = 8e-024
Identities = 18/44 (40%), Positives = 28/44 (63%)
Frame = -2 / +2
Query: 659 KGKGSVLLTAAPQCPYPDASLGPALQTGQFDNVWIQFYNNPGCA 528
+G+ V LTA P+C YPD + AL TG ++++FY++ CA
Sbjct: 170 RGRTPVQLTATPRCGYPDRHVERALATGLVGRIFVRFYDDADCA 301
Score = 40.9 bits (83), Expect(4) = 8e-024
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = -2 / +2
Query: 413 GYIDPGTLTGTVIPAIRGIGNYGGIMVWDRFND 315
GY+ P L VI ++ YGG+MVW+RF D
Sbjct: 392 GYVHPKNLYYGVIQVVQKAAIYGGVMVWERFED 490
Score = 40.5 bits (82), Expect(4) = 8e-024
Identities = 18/33 (54%), Positives = 21/33 (63%)
Frame = -2 / +2
Query: 758 GSSRPLGAAVLDGIDFDIENGQSAHYDDLANAL 660
G RP A +DGIDF IENG +YD+LA L
Sbjct: 50 GVYRPFCDAFVDGIDFFIENGAPDNYDELAKRL 148
Score = 35.4 bits (71), Expect(4) = 8e-024
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -2 / +2
Query: 506 NLVNAWNTWTSSITAGSFYLGVPASPQAAG 417
N W+ WT++ + YLG+PAS Q G
Sbjct: 305 NWQRQWDKWTAAYPSAQIYLGLPASEQKVG 394
>gb|CK122763.1|CK122763 BES1824105l04 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010L045 5-PRIME, mRNA sequence
Length = 623
Score = 59.3 bits (123), Expect(3) = 9e-024
Identities = 24/49 (48%), Positives = 31/49 (63%)
Frame = -2 / +2
Query: 908 GQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGG 762
G DI+ CQS G+ V LSIGG G Y L + A +AD+LW ++LGG
Sbjct: 269 GIGDDIRHCQSAGVTVSLSIGGFGGDYALPTNQSALDLADHLWWSYLGG 415
Score = 57.0 bits (118), Expect(3) = 9e-024
Identities = 19/44 (43%), Positives = 32/44 (72%)
Frame = -2 / +2
Query: 1094 TTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNGQ 963
T G + V+WG+N +EGSL +AC++G+Y V ++FL +G+G+
Sbjct: 104 TGKTGQLTVFWGRNKDEGSLREACDAGVYTAVIMSFLNVYGHGK 235
Score = 32.2 bits (64), Expect(3) = 9e-024
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = -2 / +2
Query: 758 GSSRPLGAAVLDGIDFDIENGQSAHYDD 675
G RP G A LDG+DF +E G + D
Sbjct: 425 GVRRPFGRARLDGVDFFLERGGPGEHYD 508
>gb|BI780772.2|BI780772 EBes01_SQ002_C13_R embryo sac, 4-6 DPA, no treatment, cv Optic,
EBes01 Hordeum vulgare subsp. vulgare cDNA clone
EBes01_SQ002_C13 5', mRNA sequence
Length = 546
Score = 59.3 bits (123), Expect(3) = 9e-024
Identities = 24/49 (48%), Positives = 31/49 (63%)
Frame = -2 / +2
Query: 908 GQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGG 762
G DI+ CQS G+ V LSIGG G Y L + A +AD+LW ++LGG
Sbjct: 215 GIGDDIRHCQSAGVTVSLSIGGFGGDYALPTNQSALDLADHLWWSYLGG 361
Score = 57.0 bits (118), Expect(3) = 9e-024
Identities = 19/44 (43%), Positives = 32/44 (72%)
Frame = -2 / +2
Query: 1094 TTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNGQ 963
T G + V+WG+N +EGSL +AC++G+Y V ++FL +G+G+
Sbjct: 50 TGKTGQLTVFWGRNKDEGSLREACDAGVYTAVIMSFLNVYGHGK 181
Score = 32.2 bits (64), Expect(3) = 9e-024
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = -2 / +2
Query: 758 GSSRPLGAAVLDGIDFDIENGQSAHYDD 675
G RP G A LDG+DF +E G + D
Sbjct: 371 GVRRPFGRARLDGVDFFLERGGPGEHYD 454
>gb|BF624578.2|BF624578 HVSMEa0017C20f Hordeum vulgare seedling shoot EST library HVcDNA0001
(Cold stress) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEa0017C20f, mRNA sequence
Length = 825
Score = 61.6 bits (128), Expect(3) = 4e-023
Identities = 23/49 (46%), Positives = 33/49 (67%)
Frame = -2 / +1
Query: 1082 GNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNGQTPVLNLAGH 936
G + V+WG+N EGSL +AC+SG+Y V ++FL FG L+L+GH
Sbjct: 118 GQVTVFWGRNKAEGSLREACDSGMYTMVTMSFLDVFGANGKYHLDLSGH 264
Score = 54.7 bits (113), Expect(3) = 4e-023
Identities = 24/65 (36%), Positives = 33/65 (50%)
Frame = -2 / +1
Query: 938 HCDPGSGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGS 759
H D + +DI+ CQ G+ V LSIGG Y L S A + D+LW+++ GGS
Sbjct: 244 HLDLSGHDLSSVGADIKHCQFKGVPVSLSIGGYGTGYSLPSNRSALDLFDHLWNSYFGGS 423
Query: 758 GSSRP 744
P
Sbjct: 424 KPGVP 438
Score = 29.9 bits (59), Expect(3) = 4e-023
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = -2 / +1
Query: 758 GSSRPLGAAVLDGIDFDIENGQSAHYDDL 672
G RP G A LDG+D +E+G A D+
Sbjct: 430 GVPRPFGDAWLDGVDLFLEHGTPADRYDV 516
>gb|BQ740058.1|BQ740058 HC04B02 HC Hordeum vulgare subsp. vulgare cDNA clone HC04B02 similar
to chitinase (EC 3.2.1.14) III C00481 - rice
gi|2696221|dbj|BAA23806.1| (D55708) chitinase [Oryza
sativa], mRNA sequence
Length = 643
Score = 61.1 bits (127), Expect(3) = 4e-023
Identities = 23/49 (46%), Positives = 33/49 (67%)
Frame = -2 / +2
Query: 1082 GNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNGQTPVLNLAGH 936
G + V+WG+N EGSL +AC+SG+Y V ++FL FG L+L+GH
Sbjct: 134 GQVTVFWGRNKAEGSLREACDSGMYTMVTMSFLDVFGAXGKYHLDLSGH 280
Score = 54.7 bits (113), Expect(3) = 4e-023
Identities = 24/65 (36%), Positives = 33/65 (50%)
Frame = -2 / +2
Query: 938 HCDPGSGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGS 759
H D + +DI+ CQ G+ V LSIGG Y L S A + D+LW+++ GGS
Sbjct: 260 HLDLSGHDLSSVGADIKHCQFKGVPVSLSIGGYGTGYSLPSNRSALDLFDHLWNSYFGGS 439
Query: 758 GSSRP 744
P
Sbjct: 440 KPGVP 454
Score = 30.4 bits (60), Expect(3) = 4e-023
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = -2 / +2
Query: 758 GSSRPLGAAVLDGIDFDIENGQSA 687
G RP G A LDG+D +E+G A
Sbjct: 446 GVPRPFGDACLDGVDLFLEHGTPA 517
>gb|BG300616.1|BG300616 HVSMEb0017L09f Hordeum vulgare seedling shoot EST library HVcDNA0002
(Dehydration stress) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEb0017L09f, mRNA sequence
Length = 872
Score = 59.3 bits (123), Expect(3) = 2e-022
Identities = 22/49 (44%), Positives = 32/49 (65%)
Frame = -2 / +2
Query: 1082 GNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNGQTPVLNLAGH 936
G++ +WGQ +GSL DAC+SG+Y V ++FL FG L+L+GH
Sbjct: 119 GHVTAFWGQTKADGSLRDACDSGMYTMVTMSFLDIFGANGKYHLDLSGH 265
Score = 54.7 bits (113), Expect(3) = 2e-022
Identities = 24/65 (36%), Positives = 33/65 (50%)
Frame = -2 / +2
Query: 938 HCDPGSGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGS 759
H D + +DI+ CQ G+ V LSIGG Y L S A + D+LW+++ GGS
Sbjct: 245 HLDLSGHDLSSVGADIKHCQFKGVPVSLSIGGYGTGYSLPSNRSALDLFDHLWNSYFGGS 424
Query: 758 GSSRP 744
P
Sbjct: 425 KPGVP 439
Score = 29.9 bits (59), Expect(3) = 2e-022
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = -2 / +2
Query: 758 GSSRPLGAAVLDGIDFDIENGQSAHYDDL 672
G RP G A LDG+D +E+G A D+
Sbjct: 431 GVPRPFGDAWLDGVDLFLEHGTPADRYDV 517
>gb|CK125329.1|CK125329 BES1824107l08 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010L087 5-PRIME, mRNA sequence
Length = 488
Score = 60.2 bits (125), Expect(3) = 2e-022
Identities = 24/49 (48%), Positives = 31/49 (63%)
Frame = -2 / +3
Query: 908 GQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGG 762
G DI+ CQS G+ V LSIGG G Y L + A +AD+LW ++LGG
Sbjct: 279 GIGDDIRHCQSAGVTVSLSIGGFGGDYALPTNQSAXDLADHLWWSYLGG 425
Score = 57.0 bits (118), Expect(3) = 2e-022
Identities = 19/44 (43%), Positives = 32/44 (72%)
Frame = -2 / +3
Query: 1094 TTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNGQ 963
T G + V+WG+N +EGSL +AC++G+Y V ++FL +G+G+
Sbjct: 114 TGKTGQLTVFWGRNKDEGSLREACDAGVYTAVIMSFLNVYGHGK 245
Score = 26.7 bits (52), Expect(3) = 2e-022
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = -2 / +3
Query: 758 GSSRPLGAAVLDGIDF 711
G RP G A LDG+DF
Sbjct: 435 GVRRPFGRARLDGVDF 482
>gb|BF259054.2|BF259054 HVSMEf0017I14f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEf0017I14f, mRNA sequence
Length = 809
Score = 61.6 bits (128), Expect(3) = 6e-022
Identities = 23/49 (46%), Positives = 33/49 (67%)
Frame = -2 / +2
Query: 1082 GNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNGQTPVLNLAGH 936
G + V+WG+N EGSL +AC+SG+Y V ++FL FG L+L+GH
Sbjct: 125 GQVTVFWGRNKAEGSLREACDSGMYTMVTMSFLDVFGANGKYHLDLSGH 271
Score = 50.6 bits (104), Expect(3) = 6e-022
Identities = 23/65 (35%), Positives = 32/65 (49%)
Frame = -2 / +2
Query: 938 HCDPGSGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGS 759
H D + +DI+ CQ G+ V LSIGG Y L S A + +LW+++ GGS
Sbjct: 251 HLDLSGHDLSSVGADIKHCQFKGVPVSLSIGGYGTGYSLPSNRSALDLFYHLWNSYFGGS 430
Query: 758 GSSRP 744
P
Sbjct: 431 KPGVP 445
Score = 29.9 bits (59), Expect(3) = 6e-022
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = -2 / +2
Query: 758 GSSRPLGAAVLDGIDFDIENGQSAHYDDL 672
G RP G A LDG+D +E+G A D+
Sbjct: 437 GVPRPFGDAWLDGVDLFLEHGTPADRYDV 523
>gb|BF625166.2|BF625166 HVSMEa0006B24f Hordeum vulgare seedling shoot EST library HVcDNA0001
(Cold stress) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEa0006B24f, mRNA sequence
Length = 775
Score = 61.6 bits (128), Expect(3) = 6e-022
Identities = 23/49 (46%), Positives = 33/49 (67%)
Frame = -2 / +2
Query: 1082 GNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNGQTPVLNLAGH 936
G + V+WG+N EGSL +AC+SG+Y V ++FL FG L+L+GH
Sbjct: 83 GQVTVFWGRNKAEGSLREACDSGMYTMVTMSFLDVFGANGKYHLDLSGH 229
Score = 54.7 bits (113), Expect(3) = 6e-022
Identities = 24/65 (36%), Positives = 33/65 (50%)
Frame = -2 / +2
Query: 938 HCDPGSGSCTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGS 759
H D + +DI+ CQ G+ V LSIGG Y L S A + D+LW+++ GGS
Sbjct: 209 HLDLSGHDLSSVGADIKHCQFKGVPVSLSIGGYGTGYSLPSNRSALDLFDHLWNSYFGGS 388
Query: 758 GSSRP 744
P
Sbjct: 389 KPGVP 403
Score = 25.8 bits (50), Expect(3) = 6e-022
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = -2 / +2
Query: 758 GSSRPLGAAVLDGIDFDIENGQSAHYDDL 672
G RP G A LDG+ +E+G A D+
Sbjct: 395 GVPRPFGDAWLDGVYLFLEHGTPADRYDV 481
>gb|BQ758987.1|BQ758987 EBma07_SQ003_N09_R maternal, 21 DPA, no treatment, cv Optic, EBma07
Hordeum vulgare subsp. vulgare cDNA clone
EBma07_SQ003_N09 5', mRNA sequence
Length = 522
Score = 52.8 bits (109), Expect(4) = 9e-021
Identities = 19/40 (47%), Positives = 26/40 (65%)
Frame = -2 / +2
Query: 413 GYIDPGTLTGTVIPAIRGIGNYGGIMVWDRFNDVQNNYSS 294
GY+ P L VI ++ NYGG+MVW+R+ D + NYSS
Sbjct: 299 GYVHPKNLYYGVIQVVQKAANYGGVMVWERYEDKRTNYSS 418
Score = 42.8 bits (87), Expect(4) = 9e-021
Identities = 17/44 (38%), Positives = 26/44 (59%)
Frame = -2 / +2
Query: 659 KGKGSVLLTAAPQCPYPDASLGPALQTGQFDNVWIQFYNNPGCA 528
+G V LTA +C YPD + AL TG ++++FY++ CA
Sbjct: 77 RGMTPVQLTATLRCGYPDRHVDLALATGLVGRIFVRFYDDADCA 208
Score = 35.4 bits (71), Expect(4) = 9e-021
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -2 / +2
Query: 506 NLVNAWNTWTSSITAGSFYLGVPASPQAAG 417
N W+ WT++ + YLG+PAS Q G
Sbjct: 212 NWQRQWDKWTAAYPSAQIYLGLPASEQKVG 301
Score = 24.0 bits (46), Expect(4) = 9e-021
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -2 / +2
Query: 713 FDIENGQSAHYDDLANAL 660
F IENG +YD+LA L
Sbjct: 2 F*IENGAPDNYDELAKRL 55
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 465,436,971
Number of Sequences: 312970
Number of extensions: 7567876
Number of successful extensions: 88864
Number of sequences better than 1.0e-020: 51
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 15128258378
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)