TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4573929.2.1
(691 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU990717.1|BU990717 HF25N16r HF Hordeum vulgare subsp. v... 185 3e-046
gb|BU995681.1|BU995681 HM11A08r HM Hordeum vulgare subsp. v... 185 3e-046
gb|CA013313.1|CA013313 HT08A11r HT Hordeum vulgare subsp. v... 185 3e-046
gb|CA021085.1|CA021085 HZ39B01r HZ Hordeum vulgare subsp. v... 185 3e-046
gb|AV936911.1|AV936911 AV936911 K. Sato unpublished cDNA li... 182 2e-045
gb|BE420919.1|BE420919 HWM003.H08 ITEC HWM Barley Leaf Libr... 164 6e-040
gb|BE558753.3|BE558753 HV_CEb0020E08f Hordeum vulgare seedl... 160 1e-038
gb|BJ449925.1|BJ449925 BJ449925 K. Sato unpublished cDNA li... 142 2e-033
gb|BJ463662.1|BJ463662 BJ463662 K. Sato unpublished cDNA li... 140 6e-033
gb|CA000191.1|CA000191 HS09J09u HS Hordeum vulgare subsp. v... 138 4e-032
gb|CA013080.1|CA013080 HT07F09r HT Hordeum vulgare subsp. v... 138 4e-032
gb|AJ462109.1|AJ462109 AJ462109 S00002 Hordeum vulgare subs... 136 1e-031
gb|BM371846.2|BM371846 EBma08_SQ004_C16_R maternal, 28 DPA,... 130 7e-030
gb|BQ766274.1|BQ766274 EBro08_SQ005_C17_R root, 3 week, dro... 126 2e-028
gb|BJ466608.1|BJ466608 BJ466608 K. Sato unpublished cDNA li... 116 1e-025
gb|BU971800.1|BU971800 HB19L11r BC Hordeum vulgare subsp. v... 109 1e-023
gb|BJ457343.1|BJ457343 BJ457343 K. Sato unpublished cDNA li... 106 1e-022
>gb|BU990717.1|BU990717 HF25N16r HF Hordeum vulgare subsp. vulgare cDNA clone HF25N16
5-PRIME, mRNA sequence
Length = 633
Score = 185 bits (398), Expect = 3e-046
Identities = 75/99 (75%), Positives = 79/99 (79%)
Frame = +1 / +3
Query: 55 IAALRKLYEDSDKIGCEVYNLGTGKGTSVLEMVAAFEKASGKKIPLVFAGRRPGDAEIVY 234
IAALRKLYEDSD+IGCEVYNLGTGKGTSVLEMVAAFEKA GKKIPLV+AGRR GDAE VY
Sbjct: 165 IAALRKLYEDSDRIGCEVYNLGTGKGTSVLEMVAAFEKACGKKIPLVYAGRRAGDAETVY 344
Query: 235 XXXXXXXXXXXXXXXYGIEEMCRDLWNWASKNPYGYAGS 351
+G+EEMCRDLWNWASKNPYGY S
Sbjct: 345 AATAKAEKELKWKAKFGVEEMCRDLWNWASKNPYGYGSS 461
Score = 149 bits (319), Expect = 2e-035
Identities = 66/94 (70%), Positives = 71/94 (75%)
Frame = -3 / -3
Query: 338 PYGFLLAQFHRSLHISSIPYXXXXXXXXXXXXXXX*TISASPGLRPANTRGIFFPEAFSN 159
P GFLLAQFHRSLHISS P T+SASP L PA TRGIFFP+AFSN
Sbjct: 448 P*GFLLAQFHRSLHISSTPNLAFHLSSFSALAVAAYTVSASPALLPA*TRGIFFPQAFSN 269
Query: 158 AATISNTDVPFPVPKLYTSQPILSESS*SFLRAA 57
AATIS+TDVPFPVP+LYTS PILSESS*SFL+AA
Sbjct: 268 AATISSTDVPFPVPRLYTSHPILSESS*SFLKAA 167
Score = 130 bits (279), Expect = 7e-030
Identities = 55/84 (65%), Positives = 60/84 (71%)
Frame = +2 / +1
Query: 59 QP*GSSTKTPTK*AVKCTTWGLERGRPCWKWWLHSRRLLGRKSLWCSLGEDPETQRSSTP 238
QP*GSSTK T+* KCT W LER RPCW+W HSRRL RKSLW L E+ E R T
Sbjct: 169 QP*GSSTKILTE*GAKCTIWALERERPCWRWLPHSRRLAERKSLWFMLEEELEMPRLCTL 348
Query: 239 QLPRQRRSSNGRPSTGSRRCAEIC 310
LP+QRRSS+GRPS G RRCAE C
Sbjct: 349 PLPKQRRSSSGRPSLGWRRCAETC 420
Score = 125 bits (267), Expect = 3e-028
Identities = 51/85 (60%), Positives = 58/85 (68%)
Frame = -1 / -1
Query: 343 RTRTGSCSPSSTDLCTSPRSRTWPSI*APSLPWQXXXXXXXXXRVFAQRTPEGFSSQKPS 164
R+R GSC PSST LCTSP +TWPS *APSL WQ ++F Q PEGFS +KPS
Sbjct: 453 RSRRGSCLPSSTGLCTSPPPQTWPST*APSLLWQWQRTQSRHLQLFFQHKPEGFSFRKPS 274
Query: 163 RMQPPFPTRTSPFQSPSCTLHSLFC 89
RM+ P P RT PFQ P CTL +LFC
Sbjct: 273 RMRQPSPARTFPFQCPDCTLRTLFC 199
>gb|BU995681.1|BU995681 HM11A08r HM Hordeum vulgare subsp. vulgare cDNA clone HM11A08
5-PRIME, mRNA sequence
Length = 642
Score = 185 bits (398), Expect = 3e-046
Identities = 75/99 (75%), Positives = 79/99 (79%)
Frame = +1 / +3
Query: 55 IAALRKLYEDSDKIGCEVYNLGTGKGTSVLEMVAAFEKASGKKIPLVFAGRRPGDAEIVY 234
IAALRKLYEDSD+IGCEVYNLGTGKGTSVLEMVAAFEKA GKKIPLV+AGRR GDAE VY
Sbjct: 159 IAALRKLYEDSDRIGCEVYNLGTGKGTSVLEMVAAFEKACGKKIPLVYAGRRAGDAETVY 338
Query: 235 XXXXXXXXXXXXXXXYGIEEMCRDLWNWASKNPYGYAGS 351
+G+EEMCRDLWNWASKNPYGY S
Sbjct: 339 AATAKAEKELKWKAKFGVEEMCRDLWNWASKNPYGYGSS 455
Score = 149 bits (319), Expect = 2e-035
Identities = 66/94 (70%), Positives = 71/94 (75%)
Frame = -3 / -3
Query: 338 PYGFLLAQFHRSLHISSIPYXXXXXXXXXXXXXXX*TISASPGLRPANTRGIFFPEAFSN 159
P GFLLAQFHRSLHISS P T+SASP L PA TRGIFFP+AFSN
Sbjct: 442 P*GFLLAQFHRSLHISSTPNLAFHLSSFSALAVAAYTVSASPALLPA*TRGIFFPQAFSN 263
Query: 158 AATISNTDVPFPVPKLYTSQPILSESS*SFLRAA 57
AATIS+TDVPFPVP+LYTS PILSESS*SFL+AA
Sbjct: 262 AATISSTDVPFPVPRLYTSHPILSESS*SFLKAA 161
Score = 130 bits (279), Expect = 7e-030
Identities = 55/84 (65%), Positives = 60/84 (71%)
Frame = +2 / +1
Query: 59 QP*GSSTKTPTK*AVKCTTWGLERGRPCWKWWLHSRRLLGRKSLWCSLGEDPETQRSSTP 238
QP*GSSTK T+* KCT W LER RPCW+W HSRRL RKSLW L E+ E R T
Sbjct: 163 QP*GSSTKILTE*GAKCTIWALERERPCWRWLPHSRRLAERKSLWFMLEEELEMPRLCTL 342
Query: 239 QLPRQRRSSNGRPSTGSRRCAEIC 310
LP+QRRSS+GRPS G RRCAE C
Sbjct: 343 PLPKQRRSSSGRPSLGWRRCAETC 414
Score = 125 bits (267), Expect = 3e-028
Identities = 51/85 (60%), Positives = 58/85 (68%)
Frame = -1 / -1
Query: 343 RTRTGSCSPSSTDLCTSPRSRTWPSI*APSLPWQXXXXXXXXXRVFAQRTPEGFSSQKPS 164
R+R GSC PSST LCTSP +TWPS *APSL WQ ++F Q PEGFS +KPS
Sbjct: 447 RSRRGSCLPSSTGLCTSPPPQTWPST*APSLLWQWQRTQSRHLQLFFQHKPEGFSFRKPS 268
Query: 163 RMQPPFPTRTSPFQSPSCTLHSLFC 89
RM+ P P RT PFQ P CTL +LFC
Sbjct: 267 RMRQPSPARTFPFQCPDCTLRTLFC 193
>gb|CA013313.1|CA013313 HT08A11r HT Hordeum vulgare subsp. vulgare cDNA clone HT08A11
5-PRIME, mRNA sequence
Length = 646
Score = 185 bits (398), Expect = 3e-046
Identities = 75/99 (75%), Positives = 79/99 (79%)
Frame = +1 / +3
Query: 55 IAALRKLYEDSDKIGCEVYNLGTGKGTSVLEMVAAFEKASGKKIPLVFAGRRPGDAEIVY 234
IAALRKLYEDSD+IGCEVYNLGTGKGTSVLEMVAAFEKA GKKIPLV+AGRR GDAE VY
Sbjct: 252 IAALRKLYEDSDRIGCEVYNLGTGKGTSVLEMVAAFEKACGKKIPLVYAGRRAGDAETVY 431
Query: 235 XXXXXXXXXXXXXXXYGIEEMCRDLWNWASKNPYGYAGS 351
+G+EEMCRDLWNWASKNPYGY S
Sbjct: 432 AATAKAEKELKWKAKFGVEEMCRDLWNWASKNPYGYGSS 548
Score = 149 bits (319), Expect = 2e-035
Identities = 66/94 (70%), Positives = 71/94 (75%)
Frame = -3 / -1
Query: 338 PYGFLLAQFHRSLHISSIPYXXXXXXXXXXXXXXX*TISASPGLRPANTRGIFFPEAFSN 159
P GFLLAQFHRSLHISS P T+SASP L PA TRGIFFP+AFSN
Sbjct: 535 P*GFLLAQFHRSLHISSTPNLAFHLSSFSALAVAAYTVSASPALLPA*TRGIFFPQAFSN 356
Query: 158 AATISNTDVPFPVPKLYTSQPILSESS*SFLRAA 57
AATIS+TDVPFPVP+LYTS PILSESS*SFL+AA
Sbjct: 355 AATISSTDVPFPVPRLYTSHPILSESS*SFLKAA 254
Score = 130 bits (279), Expect = 7e-030
Identities = 55/84 (65%), Positives = 60/84 (71%)
Frame = +2 / +1
Query: 59 QP*GSSTKTPTK*AVKCTTWGLERGRPCWKWWLHSRRLLGRKSLWCSLGEDPETQRSSTP 238
QP*GSSTK T+* KCT W LER RPCW+W HSRRL RKSLW L E+ E R T
Sbjct: 256 QP*GSSTKILTE*GAKCTIWALERERPCWRWLPHSRRLAERKSLWFMLEEELEMPRLCTL 435
Query: 239 QLPRQRRSSNGRPSTGSRRCAEIC 310
LP+QRRSS+GRPS G RRCAE C
Sbjct: 436 PLPKQRRSSSGRPSLGWRRCAETC 507
Score = 125 bits (267), Expect = 3e-028
Identities = 51/85 (60%), Positives = 58/85 (68%)
Frame = -1 / -2
Query: 343 RTRTGSCSPSSTDLCTSPRSRTWPSI*APSLPWQXXXXXXXXXRVFAQRTPEGFSSQKPS 164
R+R GSC PSST LCTSP +TWPS *APSL WQ ++F Q PEGFS +KPS
Sbjct: 540 RSRRGSCLPSSTGLCTSPPPQTWPST*APSLLWQWQRTQSRHLQLFFQHKPEGFSFRKPS 361
Query: 163 RMQPPFPTRTSPFQSPSCTLHSLFC 89
RM+ P P RT PFQ P CTL +LFC
Sbjct: 360 RMRQPSPARTFPFQCPDCTLRTLFC 286
>gb|CA021085.1|CA021085 HZ39B01r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ39B01
5-PRIME, mRNA sequence
Length = 539
Score = 185 bits (398), Expect = 3e-046
Identities = 75/99 (75%), Positives = 79/99 (79%)
Frame = +1 / +1
Query: 55 IAALRKLYEDSDKIGCEVYNLGTGKGTSVLEMVAAFEKASGKKIPLVFAGRRPGDAEIVY 234
IAALRKLYEDSD+IGCEVYNLGTGKGTSVLEMVAAFEKA GKKIPLV+AGRR GDAE VY
Sbjct: 10 IAALRKLYEDSDRIGCEVYNLGTGKGTSVLEMVAAFEKACGKKIPLVYAGRRAGDAETVY 189
Query: 235 XXXXXXXXXXXXXXXYGIEEMCRDLWNWASKNPYGYAGS 351
+G+EEMCRDLWNWASKNPYGY S
Sbjct: 190 AATAKAEKELKWKAKFGVEEMCRDLWNWASKNPYGYGSS 306
Score = 149 bits (319), Expect = 2e-035
Identities = 66/94 (70%), Positives = 71/94 (75%)
Frame = -3 / -1
Query: 338 PYGFLLAQFHRSLHISSIPYXXXXXXXXXXXXXXX*TISASPGLRPANTRGIFFPEAFSN 159
P GFLLAQFHRSLHISS P T+SASP L PA TRGIFFP+AFSN
Sbjct: 293 P*GFLLAQFHRSLHISSTPNLAFHLSSFSALAVAAYTVSASPALLPA*TRGIFFPQAFSN 114
Query: 158 AATISNTDVPFPVPKLYTSQPILSESS*SFLRAA 57
AATIS+TDVPFPVP+LYTS PILSESS*SFL+AA
Sbjct: 113 AATISSTDVPFPVPRLYTSHPILSESS*SFLKAA 12
Score = 130 bits (279), Expect = 7e-030
Identities = 55/84 (65%), Positives = 60/84 (71%)
Frame = +2 / +2
Query: 59 QP*GSSTKTPTK*AVKCTTWGLERGRPCWKWWLHSRRLLGRKSLWCSLGEDPETQRSSTP 238
QP*GSSTK T+* KCT W LER RPCW+W HSRRL RKSLW L E+ E R T
Sbjct: 14 QP*GSSTKILTE*GAKCTIWALERERPCWRWLPHSRRLAERKSLWFMLEEELEMPRLCTL 193
Query: 239 QLPRQRRSSNGRPSTGSRRCAEIC 310
LP+QRRSS+GRPS G RRCAE C
Sbjct: 194 PLPKQRRSSSGRPSLGWRRCAETC 265
Score = 125 bits (267), Expect = 3e-028
Identities = 51/85 (60%), Positives = 58/85 (68%)
Frame = -1 / -2
Query: 343 RTRTGSCSPSSTDLCTSPRSRTWPSI*APSLPWQXXXXXXXXXRVFAQRTPEGFSSQKPS 164
R+R GSC PSST LCTSP +TWPS *APSL WQ ++F Q PEGFS +KPS
Sbjct: 298 RSRRGSCLPSSTGLCTSPPPQTWPST*APSLLWQWQRTQSRHLQLFFQHKPEGFSFRKPS 119
Query: 163 RMQPPFPTRTSPFQSPSCTLHSLFC 89
RM+ P P RT PFQ P CTL +LFC
Sbjct: 118 RMRQPSPARTFPFQCPDCTLRTLFC 44
>gb|AV936911.1|AV936911 AV936911 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal8e17 3', mRNA sequence
Length = 719
Score = 182 bits (392), Expect = 2e-045
Identities = 74/99 (74%), Positives = 78/99 (78%)
Frame = +1 / -2
Query: 55 IAALRKLYEDSDKIGCEVYNLGTGKGTSVLEMVAAFEKASGKKIPLVFAGRRPGDAEIVY 234
IAAL KLYEDSD+IGCEVYNLGTGKGTSVLEMVAAFEKA GKKIPLV+AGRR GDAE VY
Sbjct: 628 IAALXKLYEDSDRIGCEVYNLGTGKGTSVLEMVAAFEKACGKKIPLVYAGRRAGDAETVY 449
Query: 235 XXXXXXXXXXXXXXXYGIEEMCRDLWNWASKNPYGYAGS 351
+G+EEMCRDLWNWASKNPYGY S
Sbjct: 448 AATAKAEKELKWKAKFGVEEMCRDLWNWASKNPYGYGSS 332
Score = 145 bits (312), Expect = 2e-034
Identities = 65/94 (69%), Positives = 70/94 (74%)
Frame = -3 / +3
Query: 338 PYGFLLAQFHRSLHISSIPYXXXXXXXXXXXXXXX*TISASPGLRPANTRGIFFPEAFSN 159
P GFLLAQFHRSLHISS P T+SASP L PA TRGIFFP+AFSN
Sbjct: 345 P*GFLLAQFHRSLHISSTPNLAFHLSSFSALAVAAYTVSASPALLPA*TRGIFFPQAFSN 524
Query: 158 AATISNTDVPFPVPKLYTSQPILSESS*SFLRAA 57
AATIS+TDVPFPVP+LYTS PILSESS*S L+AA
Sbjct: 525 AATISSTDVPFPVPRLYTSHPILSESS*SXLKAA 626
Score = 127 bits (272), Expect = 6e-029
Identities = 54/84 (64%), Positives = 59/84 (70%)
Frame = +2 / -3
Query: 59 QP*GSSTKTPTK*AVKCTTWGLERGRPCWKWWLHSRRLLGRKSLWCSLGEDPETQRSSTP 238
QP* SSTK T+* KCT W LER RPCW+W HSRRL RKSLW L E+ E R T
Sbjct: 624 QP*XSSTKILTE*GAKCTIWALERERPCWRWLPHSRRLAERKSLWFMLEEELEMPRLCTL 445
Query: 239 QLPRQRRSSNGRPSTGSRRCAEIC 310
LP+QRRSS+GRPS G RRCAE C
Sbjct: 444 PLPKQRRSSSGRPSLGWRRCAETC 373
Score = 125 bits (267), Expect = 3e-028
Identities = 51/85 (60%), Positives = 58/85 (68%)
Frame = -1 / +1
Query: 343 RTRTGSCSPSSTDLCTSPRSRTWPSI*APSLPWQXXXXXXXXXRVFAQRTPEGFSSQKPS 164
R+R GSC PSST LCTSP +TWPS *APSL WQ ++F Q PEGFS +KPS
Sbjct: 340 RSRRGSCLPSSTGLCTSPPPQTWPST*APSLLWQWQRTQSRHLQLFFQHKPEGFSFRKPS 519
Query: 163 RMQPPFPTRTSPFQSPSCTLHSLFC 89
RM+ P P RT PFQ P CTL +LFC
Sbjct: 520 RMRQPSPARTFPFQCPDCTLRTLFC 594
>gb|BE420919.1|BE420919 HWM003.H08 ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM003.H08, mRNA sequence
Length = 1212
Score = 164 bits (352), Expect = 6e-040
Identities = 69/93 (74%), Positives = 73/93 (78%)
Frame = +1 / +2
Query: 55 IAALRKLYEDSDKIGCEVYNLGTGKGTSVLEMVAAFEKASGKKIPLVFAGRRPGDAEIVY 234
IAALRKLYEDSD+IGCEVYNLGTGKGTSVLEMVAAFEKA GKKIPLV+AGRR GDAE VY
Sbjct: 365 IAALRKLYEDSDRIGCEVYNLGTGKGTSVLEMVAAFEKACGKKIPLVYAGRRAGDAETVY 544
Query: 235 XXXXXXXXXXXXXXXYGIEEMCRDLWNWASKNP 333
+ +EEMCRDLWN ASKNP
Sbjct: 545 AATAKAEKELKWKAKFRLEEMCRDLWNLASKNP 643
Score = 141 bits (302), Expect = 4e-033
Identities = 63/92 (68%), Positives = 69/92 (75%)
Frame = -3 / -1
Query: 332 GFLLAQFHRSLHISSIPYXXXXXXXXXXXXXXX*TISASPGLRPANTRGIFFPEAFSNAA 153
GFLLA+FHRSLHISS T+SASP L PA TRGIFFP+AFSNAA
Sbjct: 642 GFLLAKFHRSLHISSSRNLAFHLSSFSALAVAAYTVSASPALLPA*TRGIFFPQAFSNAA 463
Query: 152 TISNTDVPFPVPKLYTSQPILSESS*SFLRAA 57
TIS+TDVPFPVP+LYTS PILSESS*SFL+AA
Sbjct: 462 TISSTDVPFPVPRLYTSHPILSESS*SFLKAA 367
Score = 130 bits (278), Expect = 9e-030
Identities = 55/84 (65%), Positives = 60/84 (71%)
Frame = +2 / +3
Query: 59 QP*GSSTKTPTK*AVKCTTWGLERGRPCWKWWLHSRRLLGRKSLWCSLGEDPETQRSSTP 238
QP*GSSTK T+* KCT W LER RPCW+W HSRRL RKSLW L E+ E R T
Sbjct: 369 QP*GSSTKILTE*GAKCTIWALERERPCWRWLPHSRRLAERKSLWFMLEEELEMPRLCTL 548
Query: 239 QLPRQRRSSNGRPSTGSRRCAEIC 310
LP+QRRSS+GRPS G RRCAE C
Sbjct: 549 PLPKQRRSSSGRPSFGWRRCAETC 620
Score = 121 bits (258), Expect = 5e-027
Identities = 49/81 (60%), Positives = 55/81 (67%)
Frame = -1 / -2
Query: 331 GSCSPSSTDLCTSPRSRTWPSI*APSLPWQXXXXXXXXXRVFAQRTPEGFSSQKPSRMQP 152
GSC PSST LCTSP + TWPS *APSL WQ ++F Q PEGFS +KPSRM+
Sbjct: 641 GSCLPSSTGLCTSPPAETWPST*APSLLWQWQRTQSRHLQLFFQHKPEGFSFRKPSRMRQ 462
Query: 151 PFPTRTSPFQSPSCTLHSLFC 89
P P RT PFQ P CTL +LFC
Sbjct: 461 PSPARTFPFQCPDCTLRTLFC 399
>gb|BE558753.3|BE558753 HV_CEb0020E08f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0020E08f, mRNA sequence
Length = 790
Score = 160 bits (343), Expect = 1e-038
Identities = 66/96 (68%), Positives = 71/96 (73%)
Frame = +1 / +1
Query: 55 IAALRKLYEDSDKIGCEVYNLGTGKGTSVLEMVAAFEKASGKKIPLVFAGRRPGDAEIVY 234
IAALRKL++ S IGCE YNLGTGKGTSVLE+V AFEKASGKKIPLV RRPGDAEI++
Sbjct: 250 IAALRKLFDSSSNIGCEPYNLGTGKGTSVLEIVNAFEKASGKKIPLVVGQRRPGDAEILF 429
Query: 235 XXXXXXXXXXXXXXXYGIEEMCRDLWNWASKNPYGY 342
YGI EMCRD WNWASKNPYGY
Sbjct: 430 AGTGKAERELNWKAKYGITEMCRDQWNWASKNPYGY 537
Score = 127 bits (272), Expect = 6e-029
Identities = 60/102 (58%), Positives = 64/102 (62%)
Frame = -3 / -3
Query: 362 LLSRDPAYPYGFLLAQFHRSLHISSIPYXXXXXXXXXXXXXXX*TISASPGLRPANTRGI 183
L+ YP GFLLAQFH SLHIS +PY ISASPG R T GI
Sbjct: 557 LVESGDPYP*GFLLAQFHWSLHISVMPYFAFQLSSLSALPVPAKRISASPGRR*PTTSGI 378
Query: 182 FFPEAFSNAATISNTDVPFPVPKLYTSQPILSESS*SFLRAA 57
FFPEAFSNA TISNTDVPFPVP+LY S P+L E S SF AA
Sbjct: 377 FFPEAFSNAFTISNTDVPFPVPRLYGSHPMLDELSKSFRSAA 252
>gb|BJ449925.1|BJ449925 BJ449925 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak27e10 5', mRNA sequence
Length = 588
Score = 142 bits (304), Expect = 2e-033
Identities = 58/83 (69%), Positives = 61/83 (73%)
Frame = +1 / +3
Query: 94 IGCEVYNLGTGKGTSVLEMVAAFEKASGKKIPLVFAGRRPGDAEIVYXXXXXXXXXXXXX 273
IGCE YNLGTGKGTSVLE+V AFEKASGKKIPLV RRPGDAEI++
Sbjct: 3 IGCEPYNLGTGKGTSVLEIVNAFEKASGKKIPLVVGQRRPGDAEILFAGTGKAERELNWK 182
Query: 274 XXYGIEEMCRDLWNWASKNPYGY 342
YGI EMCRD WNWASKNPYGY
Sbjct: 183 AKYGITEMCRDQWNWASKNPYGY 251
Score = 117 bits (250), Expect = 7e-026
Identities = 53/90 (58%), Positives = 57/90 (63%)
Frame = -3 / -3
Query: 362 LLSRDPAYPYGFLLAQFHRSLHISSIPYXXXXXXXXXXXXXXX*TISASPGLRPANTRGI 183
L+ YP GFLLAQFH SLHIS +PY ISASPG R T GI
Sbjct: 271 LVESGDPYP*GFLLAQFHWSLHISVMPYFAFQLSSLSALPVPAKRISASPGRR*PTTSGI 92
Query: 182 FFPEAFSNAATISNTDVPFPVPKLYTSQPI 93
FFPEAFSNA TISNTDVPFPVP+LY S P+
Sbjct: 91 FFPEAFSNAFTISNTDVPFPVPRLYGSHPM 2
>gb|BJ463662.1|BJ463662 BJ463662 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags32p11 5', mRNA sequence
Length = 540
Score = 140 bits (301), Expect = 6e-033
Identities = 59/89 (66%), Positives = 64/89 (71%)
Frame = +1 / +1
Query: 55 IAALRKLYEDSDKIGCEVYNLGTGKGTSVLEMVAAFEKASGKKIPLVFAGRRPGDAEIVY 234
IAALRKL++ S IGCE YNLGTGKGTSVLE+V AFEKASGKKIPLV RRPGDAEI++
Sbjct: 274 IAALRKLFDSSSNIGCEPYNLGTGKGTSVLEIVNAFEKASGKKIPLVVGQRRPGDAEILF 453
Query: 235 XXXXXXXXXXXXXXXYGIEEMCRDLWNWA 321
YGI EMCRD WNWA
Sbjct: 454 AGTGKAERELNWKAKYGITEMCRDQWNWA 540
Score = 113 bits (241), Expect = 1e-024
Identities = 53/88 (60%), Positives = 56/88 (63%)
Frame = -3 / -2
Query: 320 AQFHRSLHISSIPYXXXXXXXXXXXXXXX*TISASPGLRPANTRGIFFPEAFSNAATISN 141
AQFH SLHIS +PY ISASPG R T GIFFPEAFSNA TISN
Sbjct: 539 AQFHWSLHISVMPYFAFQLSSLSALPVPAKRISASPGRR*PTTSGIFFPEAFSNAFTISN 360
Query: 140 TDVPFPVPKLYTSQPILSESS*SFLRAA 57
TDVPFPVP+LY S P+L E S SF AA
Sbjct: 359 TDVPFPVPRLYGSHPMLDELSKSFRSAA 276
>gb|CA000191.1|CA000191 HS09J09u HS Hordeum vulgare subsp. vulgare cDNA clone HS09J09
3-PRIME, mRNA sequence
Length = 549
Score = 138 bits (295), Expect = 4e-032
Identities = 55/91 (60%), Positives = 61/91 (67%)
Frame = +1 / -2
Query: 94 IGCEVYNLGTGKGTSVLEMVAAFEKASGKKIPLVFAGRRPGDAEIVYXXXXXXXXXXXXX 273
IGC YNLGTG+GT+VLEMV+AFEKA GKKIP+ RRPGD+E VY
Sbjct: 473 IGCVAYNLGTGRGTTVLEMVSAFEKAYGKKIPVKMCPRRPGDSEQVYASTAKAEQELGWR 294
Query: 274 XXYGIEEMCRDLWNWASKNPYGYAGSRDNSK 366
YGIEEMCRD WNWA KNPYGY G +K
Sbjct: 293 AKYGIEEMCRDQWNWARKNPYGYCGDAAENK 201
Score = 102 bits (218), Expect = 2e-021
Identities = 48/91 (52%), Positives = 53/91 (58%)
Frame = -3 / +1
Query: 365 LLLSRDPAYPYGFLLAQFHRSLHISSIPYXXXXXXXXXXXXXXX*TISASPGLRPANTRG 186
L + P PYGF LAQFH SLHISSIPY T S SPGL G
Sbjct: 202 LFSAASPQ*PYGFFLAQFHWSLHISSIPYLALQPSSCSALAVDAYTCSESPGLLGHIFTG 381
Query: 185 IFFPEAFSNAATISNTDVPFPVPKLYTSQPI 93
IF P AFSNA TIS+T VP PVP+LY +QP+
Sbjct: 382 IFLPYAFSNALTISSTVVPRPVPRLYATQPM 474
>gb|CA013080.1|CA013080 HT07F09r HT Hordeum vulgare subsp. vulgare cDNA clone HT07F09
5-PRIME, mRNA sequence
Length = 637
Score = 138 bits (295), Expect = 4e-032
Identities = 55/91 (60%), Positives = 61/91 (67%)
Frame = +1 / +1
Query: 94 IGCEVYNLGTGKGTSVLEMVAAFEKASGKKIPLVFAGRRPGDAEIVYXXXXXXXXXXXXX 273
IGC YNLGTG+GT+VLEMV+AFEKA GKKIP+ RRPGD+E VY
Sbjct: 298 IGCVAYNLGTGRGTTVLEMVSAFEKAYGKKIPVKMCPRRPGDSEQVYASTAKAEQELGWR 477
Query: 274 XXYGIEEMCRDLWNWASKNPYGYAGSRDNSK 366
YGIEEMCRD WNWA KNPYGY G +K
Sbjct: 478 AKYGIEEMCRDQWNWARKNPYGYCGDAAENK 570
Score = 102 bits (218), Expect = 2e-021
Identities = 48/91 (52%), Positives = 53/91 (58%)
Frame = -3 / -3
Query: 365 LLLSRDPAYPYGFLLAQFHRSLHISSIPYXXXXXXXXXXXXXXX*TISASPGLRPANTRG 186
L + P PYGF LAQFH SLHISSIPY T S SPGL G
Sbjct: 569 LFSAASPQ*PYGFFLAQFHWSLHISSIPYLALQPSSCSALAVDAYTCSESPGLLGHIFTG 390
Query: 185 IFFPEAFSNAATISNTDVPFPVPKLYTSQPI 93
IF P AFSNA TIS+T VP PVP+LY +QP+
Sbjct: 389 IFLPYAFSNALTISSTVVPRPVPRLYATQPM 297
>gb|AJ462109.1|AJ462109 AJ462109 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200070B09F1, mRNA sequence
Length = 360
Score = 136 bits (291), Expect = 1e-031
Identities = 54/90 (60%), Positives = 60/90 (66%)
Frame = +1 / +1
Query: 97 GCEVYNLGTGKGTSVLEMVAAFEKASGKKIPLVFAGRRPGDAEIVYXXXXXXXXXXXXXX 276
GC YNLGTG+GT+VLEMV+AFEKA GKKIP+ RRPGD+E VY
Sbjct: 13 GCVAYNLGTGRGTTVLEMVSAFEKAYGKKIPVKMCPRRPGDSEQVYASTAKAEQELGWRA 192
Query: 277 XYGIEEMCRDLWNWASKNPYGYAGSRDNSK 366
YGIEEMCRD WNWA KNPYGY G +K
Sbjct: 193 KYGIEEMCRDQWNWARKNPYGYCGDAAENK 282
>gb|BM371846.2|BM371846 EBma08_SQ004_C16_R maternal, 28 DPA, no treatment, cv Optic, EBma08
Hordeum vulgare subsp. vulgare cDNA clone
EBma08_SQ004_C16 5', mRNA sequence
Length = 398
Score = 130 bits (279), Expect = 7e-030
Identities = 55/60 (91%), Positives = 57/60 (95%)
Frame = +1 / +1
Query: 55 IAALRKLYEDSDKIGCEVYNLGTGKGTSVLEMVAAFEKASGKKIPLVFAGRRPGDAEIVY 234
IAALRKLYEDSD+IGCEVYNLGTGKGTSVLEMVAAFEKA GKKIPLV+AGRR GDAE VY
Sbjct: 208 IAALRKLYEDSDRIGCEVYNLGTGKGTSVLEMVAAFEKACGKKIPLVYAGRRAGDAETVY 387
Score = 114 bits (244), Expect = 4e-025
Identities = 49/58 (84%), Positives = 54/58 (93%)
Frame = -3 / -1
Query: 230 TISASPGLRPANTRGIFFPEAFSNAATISNTDVPFPVPKLYTSQPILSESS*SFLRAA 57
T+SASP L PA TRGIFFP+AFSNAATIS+TDVPFPVP+LYTS PILSESS*SFL+AA
Sbjct: 383 TVSASPALLPA*TRGIFFPQAFSNAATISSTDVPFPVPRLYTSHPILSESS*SFLKAA 210
>gb|BQ766274.1|BQ766274 EBro08_SQ005_C17_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ005_C17 5', mRNA sequence
Length = 639
Score = 126 bits (269), Expect = 2e-028
Identities = 52/76 (68%), Positives = 55/76 (72%)
Frame = +1 / +1
Query: 115 LGTGKGTSVLEMVAAFEKASGKKIPLVFAGRRPGDAEIVYXXXXXXXXXXXXXXXYGIEE 294
LGTGKGTSVLE+V AFEKASGKKIPLV RRPGDAEI++ YGI E
Sbjct: 1 LGTGKGTSVLEIVNAFEKASGKKIPLVVGQRRPGDAEILFAGTGKAERELNWKAKYGITE 180
Query: 295 MCRDLWNWASKNPYGY 342
MCRD WNWASKNPYGY
Sbjct: 181 MCRDQWNWASKNPYGY 228
Score = 106 bits (227), Expect = 1e-022
Identities = 49/82 (59%), Positives = 51/82 (62%)
Frame = -3 / -2
Query: 362 LLSRDPAYPYGFLLAQFHRSLHISSIPYXXXXXXXXXXXXXXX*TISASPGLRPANTRGI 183
L+ YP GFLLAQFH SLHIS +PY ISASPG R T GI
Sbjct: 248 LVESGDPYP*GFLLAQFHWSLHISVMPYFAFQLSSLSALPVPAKRISASPGRR*PTTSGI 69
Query: 182 FFPEAFSNAATISNTDVPFPVP 117
FFPEAFSNA TISNTDVPFPVP
Sbjct: 68 FFPEAFSNAFTISNTDVPFPVP 3
>gb|BJ466608.1|BJ466608 BJ466608 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags32p11 3', mRNA sequence
Length = 667
Score = 116 bits (248), Expect = 1e-025
Identities = 48/72 (66%), Positives = 51/72 (70%)
Frame = +1 / -3
Query: 127 KGTSVLEMVAAFEKASGKKIPLVFAGRRPGDAEIVYXXXXXXXXXXXXXXXYGIEEMCRD 306
KGTSVLE+V AFEKASGKKIPLV RRPGDAEI++ YGI EMCRD
Sbjct: 665 KGTSVLEIVNAFEKASGKKIPLVVGQRRPGDAEILFAGTGKAERELNWKAKYGITEMCRD 486
Query: 307 LWNWASKNPYGY 342
WNWASKNPYGY
Sbjct: 485 QWNWASKNPYGY 450
>gb|BU971800.1|BU971800 HB19L11r BC Hordeum vulgare subsp. vulgare cDNA clone HB19L11
5-PRIME, mRNA sequence
Length = 569
Score = 109 bits (233), Expect = 1e-023
Identities = 46/49 (93%), Positives = 48/49 (97%)
Frame = +1 / +1
Query: 55 IAALRKLYEDSDKIGCEVYNLGTGKGTSVLEMVAAFEKASGKKIPLVFA 201
IAALRKLYEDSD+IGCEVYNLGTGKGTSVLEMVAAFEKA GKKIPLV+A
Sbjct: 421 IAALRKLYEDSDRIGCEVYNLGTGKGTSVLEMVAAFEKACGKKIPLVYA 567
Score = 101 bits (215), Expect = 4e-021
Identities = 43/49 (87%), Positives = 47/49 (95%)
Frame = -3 / -1
Query: 203 PANTRGIFFPEAFSNAATISNTDVPFPVPKLYTSQPILSESS*SFLRAA 57
PA TRGIFFP+AFSNAATIS+TDVPFPVP+LYTS PILSESS*SFL+AA
Sbjct: 569 PA*TRGIFFPQAFSNAATISSTDVPFPVPRLYTSHPILSESS*SFLKAA 423
>gb|BJ457343.1|BJ457343 BJ457343 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak27e10 3', mRNA sequence
Length = 579
Score = 106 bits (226), Expect = 1e-022
Identities = 44/69 (63%), Positives = 47/69 (68%)
Frame = +1 / -2
Query: 136 SVLEMVAAFEKASGKKIPLVFAGRRPGDAEIVYXXXXXXXXXXXXXXXYGIEEMCRDLWN 315
SVL +V AFEKASGKKIPLV RRPGDAEI++ YGI EMCRD WN
Sbjct: 578 SVLXIVNAFEKASGKKIPLVVGQRRPGDAEILFAGTGKAERELNWKAKYGITEMCRDQWN 399
Query: 316 WASKNPYGY 342
WASKNPYGY
Sbjct: 398 WASKNPYGY 372
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 308,629,588
Number of Sequences: 312970
Number of extensions: 5174071
Number of successful extensions: 88381
Number of sequences better than 1.0e-020: 25
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7550174202
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)