TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4534747.2.1
(628 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ660212.1|BQ660212 HI01I09w HI Hordeum vulgare subsp. v... 138 3e-057
gb|BQ470446.1|BQ470446 HX03A05r HX Hordeum vulgare subsp. v... 144 4e-055
gb|BQ470603.1|BQ470603 HX03H14r HX Hordeum vulgare subsp. v... 144 4e-055
gb|BQ465179.1|BQ465179 HU02N24r HU Hordeum vulgare subsp. v... 144 4e-055
gb|CA007256.1|CA007256 HU07F09r HU Hordeum vulgare subsp. v... 144 4e-055
gb|CB874708.1|CB874708 HX06B10w HX Hordeum vulgare subsp. v... 109 1e-045
gb|BU995645.1|BU995645 HM10O13r HM Hordeum vulgare subsp. v... 102 3e-044
gb|BJ544669.1|BJ544669 BJ544669 K. Sato unpublished cDNA li... 104 9e-038
gb|BE421430.1|BE421430 HWM009.C03 ITEC HWM Barley Leaf Libr... 103 1e-037
gb|AV926878.1|AV926878 AV926878 K. Sato unpublished cDNA li... 103 1e-037
gb|BU997959.1|BU997959 HI09I16r HI Hordeum vulgare subsp. v... 103 1e-037
gb|BF626879.2|BF626879 HVSMEb0001J09f Hordeum vulgare seedl... 103 1e-036
gb|BJ546140.1|BJ546140 BJ546140 K. Sato unpublished cDNA li... 91 8e-034
gb|BF254638.2|BF254638 HVSMEf0004J12f Hordeum vulgare seedl... 138 3e-032
gb|CB881798.1|CB881798 HM10O13w HM Hordeum vulgare subsp. v... 102 2e-030
gb|BJ547950.1|BJ547950 BJ547950 K. Sato unpublished cDNA li... 77 1e-029
gb|BJ548315.1|BJ548315 BJ548315 K. Sato unpublished cDNA li... 77 1e-029
gb|BJ546986.1|BJ546986 BJ546986 K. Sato unpublished cDNA li... 77 1e-029
gb|AV834352.1|AV834352 AV834352 K. Sato unpublished cDNA li... 90 3e-029
gb|AJ462548.1|AJ462548 AJ462548 S00002 Hordeum vulgare subs... 109 1e-026
gb|BF622824.2|BF622824 HVSMEa0009C21f Hordeum vulgare seedl... 95 1e-026
gb|BG415060.1|BG415060 HVSMEk0004P24f Hordeum vulgare testa... 102 2e-026
gb|BJ546987.1|BJ546987 BJ546987 K. Sato unpublished cDNA li... 73 6e-025
gb|AV919193.1|AV919193 AV919193 K. Sato unpublished cDNA li... 73 8e-025
gb|CB875785.1|CB875785 HX09F12w HX Hordeum vulgare subsp. v... 103 7e-024
gb|BJ547677.1|BJ547677 BJ547677 K. Sato unpublished cDNA li... 73 2e-021
gb|AV832731.1|AV832731 AV832731 K. Sato unpublished cDNA li... 73 2e-021
>gb|BQ660212.1|BQ660212 HI01I09w HI Hordeum vulgare subsp. vulgare cDNA clone HI01I09
3-PRIME, mRNA sequence
Length = 677
Score = 138 bits (297), Expect(2) = 3e-057
Identities = 53/63 (84%), Positives = 57/63 (90%)
Frame = +2 / -3
Query: 80 FVGTHEYLAPEIVRGEGHGSAVDWWTFGIFLYELLHGTTPFKGDSNRATLCNVVDQPLRF 259
FVGTHEYLAPEI++GEGHGSAVDWWTFGIFLYELL G TPFKG NRATL NVV QPLRF
Sbjct: 675 FVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVVGQPLRF 496
Query: 260 PDA 268
P++
Sbjct: 495 PES 487
Score = 107 bits (229), Expect(2) = 5e-032
Identities = 45/62 (72%), Positives = 49/62 (79%)
Frame = -2 / +2
Query: 264 SGNRSGWSTTLHSVARLLSPLNGVVPWRSSYRKMPKVHQSTALPWPSPLTISGARYSCVP 85
SGN GW TTL+SVA PLNGV+P RSSY+ MPKVHQSTALP PSP ISGA+YS VP
Sbjct: 491 SGNLRGWPTTLNSVALFPEPLNGVLPKRSSYKNMPKVHQSTALPCPSPFMISGAKYSWVP 670
Query: 84 TN 79
TN
Sbjct: 671 TN 676
Score = 102 bits (218), Expect(2) = 3e-057
Identities = 39/60 (65%), Positives = 48/60 (80%)
Frame = +2 / -3
Query: 284 VSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDPADLGR 463
V ARDLIRGLL+K+PQ R+A +RGA EIKQHPFF+GVNWAL+R A PP +P P ++ R
Sbjct: 480 VGFAARDLIRGLLIKEPQHRLAYKRGATEIKQHPFFEGVNWALIRCATPPDIPKPVEIPR 301
Score = 49.6 bits (102), Expect(2) = 5e-032
Identities = 23/37 (62%), Positives = 24/37 (64%)
Frame = -2 / +2
Query: 405 QLTPSKNGCCLISAAPLLVAILFWGSFTRSPRMRSRA 295
QLTPSKNGCC IS AP L A GS +P MRS A
Sbjct: 359 QLTPSKNGCCFISVAPRL*ANRCCGSLISNPLMRSLA 469
>gb|BQ470446.1|BQ470446 HX03A05r HX Hordeum vulgare subsp. vulgare cDNA clone HX03A05
5-PRIME, mRNA sequence
Length = 666
Score = 144 bits (309), Expect(2) = 4e-055
Identities = 55/72 (76%), Positives = 62/72 (86%)
Frame = +2 / +2
Query: 53 EPTDARSTSFVGTHEYLAPEIVRGEGHGSAVDWWTFGIFLYELLHGTTPFKGDSNRATLC 232
EPTDARS SFVGTHEYLAPEI+RG+GHGS+VDWWT GIFLYELL+G TPF+G N TL
Sbjct: 122 EPTDARSNSFVGTHEYLAPEIIRGDGHGSSVDWWTLGIFLYELLYGKTPFRGPGNDETLT 301
Query: 233 NVVDQPLRFPDA 268
NVV Q L+FPD+
Sbjct: 302 NVVSQGLKFPDS 337
Score = 107 bits (229), Expect(2) = 1e-030
Identities = 48/71 (67%), Positives = 54/71 (76%)
Frame = -2 / -1
Query: 264 SGNRSGWSTTLHSVARLLSPLNGVVPWRSSYRKMPKVHQSTALPWPSPLTISGARYSCVP 85
SGN S TT SV+ L PLNGV+P +S RK+P+VHQST LPWPSPL ISGARYS VP
Sbjct: 333 SGNFSPCETTFVSVSSLPGPLNGVLP*SNS*RKIPRVHQSTELPWPSPLMISGARYSWVP 154
Query: 84 TNDVDRASVGS 52
TN+ D ASVGS
Sbjct: 153 TNEFDLASVGS 121
Score = 90.4 bits (191), Expect(2) = 4e-055
Identities = 35/53 (66%), Positives = 43/53 (81%)
Frame = +2 / +2
Query: 284 VSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVP 442
VS ARDLIRGLLVK+P+ R+ +R+GAAEIK+HPFF G+NWAL+R PP P
Sbjct: 344 VSYQARDLIRGLLVKEPEHRLGSRKGAAEIKRHPFFQGLNWALIRWTAPPETP 502
Score = 45.1 bits (92), Expect(2) = 1e-030
Identities = 23/41 (56%), Positives = 25/41 (60%)
Frame = -2 / -1
Query: 405 QLTPSKNGCCLISAAPLLVAILFWGSFTRSPRMRSRATVLT 283
Q P K GC LISAAP L GSFT +P +RSRA LT
Sbjct: 465 QFRPWKKGCRLISAAPFLEPSRCSGSFTSNPLIRSRAW*LT 343
>gb|BQ470603.1|BQ470603 HX03H14r HX Hordeum vulgare subsp. vulgare cDNA clone HX03H14
5-PRIME, mRNA sequence
Length = 629
Score = 144 bits (309), Expect(2) = 4e-055
Identities = 55/72 (76%), Positives = 62/72 (86%)
Frame = +2 / +2
Query: 53 EPTDARSTSFVGTHEYLAPEIVRGEGHGSAVDWWTFGIFLYELLHGTTPFKGDSNRATLC 232
EPTDARS SFVGTHEYLAPEI+RG+GHGS+VDWWT GIFLYELL+G TPF+G N TL
Sbjct: 122 EPTDARSNSFVGTHEYLAPEIIRGDGHGSSVDWWTLGIFLYELLYGKTPFRGPGNDETLT 301
Query: 233 NVVDQPLRFPDA 268
NVV Q L+FPD+
Sbjct: 302 NVVSQGLKFPDS 337
Score = 107 bits (229), Expect(2) = 1e-030
Identities = 48/71 (67%), Positives = 54/71 (76%)
Frame = -2 / -3
Query: 264 SGNRSGWSTTLHSVARLLSPLNGVVPWRSSYRKMPKVHQSTALPWPSPLTISGARYSCVP 85
SGN S TT SV+ L PLNGV+P +S RK+P+VHQST LPWPSPL ISGARYS VP
Sbjct: 333 SGNFSPCETTFVSVSSLPGPLNGVLP*SNS*RKIPRVHQSTELPWPSPLMISGARYSWVP 154
Query: 84 TNDVDRASVGS 52
TN+ D ASVGS
Sbjct: 153 TNEFDLASVGS 121
Score = 90.4 bits (191), Expect(2) = 4e-055
Identities = 35/53 (66%), Positives = 43/53 (81%)
Frame = +2 / +2
Query: 284 VSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVP 442
VS ARDLIRGLLVK+P+ R+ +R+GAAEIK+HPFF G+NWAL+R PP P
Sbjct: 344 VSYQARDLIRGLLVKEPEHRLGSRKGAAEIKRHPFFQGLNWALIRWTAPPETP 502
Score = 45.1 bits (92), Expect(2) = 1e-030
Identities = 23/41 (56%), Positives = 25/41 (60%)
Frame = -2 / -3
Query: 405 QLTPSKNGCCLISAAPLLVAILFWGSFTRSPRMRSRATVLT 283
Q P K GC LISAAP L GSFT +P +RSRA LT
Sbjct: 465 QFRPWKKGCRLISAAPFLEPSRCSGSFTSNPLIRSRAW*LT 343
>gb|BQ465179.1|BQ465179 HU02N24r HU Hordeum vulgare subsp. vulgare cDNA clone HU02N24
5-PRIME, mRNA sequence
Length = 624
Score = 144 bits (309), Expect(2) = 4e-055
Identities = 55/72 (76%), Positives = 62/72 (86%)
Frame = +2 / +2
Query: 53 EPTDARSTSFVGTHEYLAPEIVRGEGHGSAVDWWTFGIFLYELLHGTTPFKGDSNRATLC 232
EPTDARS SFVGTHEYLAPEI+RG+GHGS+VDWWT GIFLYELL+G TPF+G N TL
Sbjct: 224 EPTDARSNSFVGTHEYLAPEIIRGDGHGSSVDWWTLGIFLYELLYGKTPFRGPGNDETLT 403
Query: 233 NVVDQPLRFPDA 268
NVV Q L+FPD+
Sbjct: 404 NVVSQGLKFPDS 439
Score = 107 bits (229), Expect(2) = 1e-030
Identities = 48/71 (67%), Positives = 54/71 (76%)
Frame = -2 / -1
Query: 264 SGNRSGWSTTLHSVARLLSPLNGVVPWRSSYRKMPKVHQSTALPWPSPLTISGARYSCVP 85
SGN S TT SV+ L PLNGV+P +S RK+P+VHQST LPWPSPL ISGARYS VP
Sbjct: 435 SGNFSPCETTFVSVSSLPGPLNGVLP*SNS*RKIPRVHQSTELPWPSPLMISGARYSWVP 256
Query: 84 TNDVDRASVGS 52
TN+ D ASVGS
Sbjct: 255 TNEFDLASVGS 223
Score = 90.4 bits (191), Expect(2) = 4e-055
Identities = 35/53 (66%), Positives = 43/53 (81%)
Frame = +2 / +2
Query: 284 VSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVP 442
VS ARDLIRGLLVK+P+ R+ +R+GAAEIK+HPFF G+NWAL+R PP P
Sbjct: 446 VSYQARDLIRGLLVKEPEHRLGSRKGAAEIKRHPFFQGLNWALIRWTAPPETP 604
Score = 45.1 bits (92), Expect(2) = 1e-030
Identities = 23/41 (56%), Positives = 25/41 (60%)
Frame = -2 / -1
Query: 405 QLTPSKNGCCLISAAPLLVAILFWGSFTRSPRMRSRATVLT 283
Q P K GC LISAAP L GSFT +P +RSRA LT
Sbjct: 567 QFRPWKKGCRLISAAPFLEPSRCSGSFTSNPLIRSRAW*LT 445
>gb|CA007256.1|CA007256 HU07F09r HU Hordeum vulgare subsp. vulgare cDNA clone HU07F09
5-PRIME, mRNA sequence
Length = 592
Score = 144 bits (309), Expect(2) = 4e-055
Identities = 55/72 (76%), Positives = 62/72 (86%)
Frame = +2 / +2
Query: 53 EPTDARSTSFVGTHEYLAPEIVRGEGHGSAVDWWTFGIFLYELLHGTTPFKGDSNRATLC 232
EPTDARS SFVGTHEYLAPEI+RG+GHGS+VDWWT GIFLYELL+G TPF+G N TL
Sbjct: 59 EPTDARSNSFVGTHEYLAPEIIRGDGHGSSVDWWTLGIFLYELLYGKTPFRGPGNDETLT 238
Query: 233 NVVDQPLRFPDA 268
NVV Q L+FPD+
Sbjct: 239 NVVSQGLKFPDS 274
Score = 107 bits (229), Expect(2) = 1e-030
Identities = 48/71 (67%), Positives = 54/71 (76%)
Frame = -2 / -2
Query: 264 SGNRSGWSTTLHSVARLLSPLNGVVPWRSSYRKMPKVHQSTALPWPSPLTISGARYSCVP 85
SGN S TT SV+ L PLNGV+P +S RK+P+VHQST LPWPSPL ISGARYS VP
Sbjct: 270 SGNFSPCETTFVSVSSLPGPLNGVLP*SNS*RKIPRVHQSTELPWPSPLMISGARYSWVP 91
Query: 84 TNDVDRASVGS 52
TN+ D ASVGS
Sbjct: 90 TNEFDLASVGS 58
Score = 90.4 bits (191), Expect(2) = 4e-055
Identities = 35/53 (66%), Positives = 43/53 (81%)
Frame = +2 / +2
Query: 284 VSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVP 442
VS ARDLIRGLLVK+P+ R+ +R+GAAEIK+HPFF G+NWAL+R PP P
Sbjct: 281 VSYQARDLIRGLLVKEPEHRLGSRKGAAEIKRHPFFQGLNWALIRWTAPPETP 439
Score = 45.1 bits (92), Expect(2) = 1e-030
Identities = 23/41 (56%), Positives = 25/41 (60%)
Frame = -2 / -2
Query: 405 QLTPSKNGCCLISAAPLLVAILFWGSFTRSPRMRSRATVLT 283
Q P K GC LISAAP L GSFT +P +RSRA LT
Sbjct: 402 QFRPWKKGCRLISAAPFLEPSRCSGSFTSNPLIRSRAW*LT 280
>gb|CB874708.1|CB874708 HX06B10w HX Hordeum vulgare subsp. vulgare cDNA clone HX06B10
3-PRIME, mRNA sequence
Length = 585
Score = 109 bits (232), Expect(3) = 1e-045
Identities = 44/90 (48%), Positives = 57/90 (63%)
Frame = +2 / -2
Query: 284 VSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDPADLGR 463
VS ARDLIRGLLVK+PQ+R+ +RGA EIKQHPFF+GVNWAL+R A PP VP P D+ R
Sbjct: 446 VSFSARDLIRGLLVKEPQQRLGCKRGATEIKQHPFFEGVNWALIRCASPPEVPKPVDIER 267
Query: 464 VASKEXXXXXXXXXXXXXXXXXXTGNFVQY 553
+K + N++++
Sbjct: 266 QTTKLPVSTSEASAAPTGASQKGSDNYLEF 177
Score = 92.3 bits (195), Expect(3) = 1e-045
Identities = 35/43 (81%), Positives = 37/43 (86%)
Frame = +2 / -2
Query: 137 SAVDWWTFGIFLYELLHGTTPFKGDSNRATLCNVVDQPLRFPD 265
SAVDWWTFGIFLYELL G TPFKG NRATL NV+ QPLRFP+
Sbjct: 584 SAVDWWTFGIFLYELLFGXTPFKGSGNRATLFNVIGQPLRFPE 456
Score = 66.1 bits (138), Expect(2) = 7e-021
Identities = 29/43 (67%), Positives = 34/43 (79%)
Frame = -2 / +1
Query: 264 SGNRSGWSTTLHSVARLLSPLNGVVPWRSSYRKMPKVHQSTAL 136
SG R+G TL+SVARL PL GV+P RSSY+ +PKVHQSTAL
Sbjct: 457 SGKRNG*PMTLNSVARLPEPLKGVLPNRSSYKNIPKVHQSTAL 585
Score = 53.8 bits (111), Expect(2) = 7e-021
Identities = 23/41 (56%), Positives = 25/41 (60%)
Frame = -2 / +1
Query: 405 QLTPSKNGCCLISAAPLLVAILFWGSFTRSPRMRSRATVLT 283
Q TPSKNGCC IS AP L WGS T P ++S A LT
Sbjct: 325 QFTPSKNGCCFISVAPRLQPNRCWGSLTSRPLIKSLAEKLT 447
Score = 21.7 bits (41), Expect(3) = 1e-045
Identities = 7/14 (50%), Positives = 9/14 (64%)
Frame = +1 / -1
Query: 541 FCSVFLGCLYIMFL 582
FCS +GC +FL
Sbjct: 42 FCSEQIGCFRFLFL 1
>gb|BU995645.1|BU995645 HM10O13r HM Hordeum vulgare subsp. vulgare cDNA clone HM10O13
5-PRIME, mRNA sequence
Length = 617
Score = 102 bits (218), Expect(2) = 3e-044
Identities = 39/60 (65%), Positives = 48/60 (80%)
Frame = +2 / +3
Query: 284 VSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDPADLGR 463
V ARDLIRGLL+K+PQ R+A +RGA EIKQHPFF+GVNWAL+R A PP +P P ++ R
Sbjct: 177 VGFAARDLIRGLLIKEPQHRLAYKRGATEIKQHPFFEGVNWALIRCATPPDIPKPVEIPR 356
Score = 95.5 bits (202), Expect(2) = 3e-044
Identities = 37/45 (82%), Positives = 39/45 (86%)
Frame = +2 / +3
Query: 134 GSAVDWWTFGIFLYELLHGTTPFKGDSNRATLCNVVDQPLRFPDA 268
GSAVDWWTFGIFLYELL G TPFKG NRATL NVV QPLRFP++
Sbjct: 36 GSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVVGQPLRFPES 170
Score = 77.6 bits (163), Expect(2) = 5e-023
Identities = 32/44 (72%), Positives = 35/44 (79%)
Frame = -2 / -2
Query: 264 SGNRSGWSTTLHSVARLLSPLNGVVPWRSSYRKMPKVHQSTALP 133
SGN GW TTL+SVA PLNGV+P RSSY+ MPKVHQSTALP
Sbjct: 166 SGNLRGWPTTLNSVALFPEPLNGVLPKRSSYKNMPKVHQSTALP 35
Score = 49.6 bits (102), Expect(2) = 5e-023
Identities = 23/37 (62%), Positives = 24/37 (64%)
Frame = -2 / -2
Query: 405 QLTPSKNGCCLISAAPLLVAILFWGSFTRSPRMRSRA 295
QLTPSKNGCC IS AP L A GS +P MRS A
Sbjct: 298 QLTPSKNGCCFISVAPRL*ANRCCGSLISNPLMRSLA 188
>gb|BJ544669.1|BJ544669 BJ544669 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak34b18 5', mRNA sequence
Length = 639
Score = 104 bits (221), Expect(2) = 9e-038
Identities = 36/68 (52%), Positives = 49/68 (72%)
Frame = +2 / +3
Query: 71 STSFVGTHEYLAPEIVRGEGHGSAVDWWTFGIFLYELLHGTTPFKGDSNRATLCNVVDQP 250
S SFVGT EY+APE++ G GH SA+DWW GI LYE+L+G TPF+G + + T N++ +
Sbjct: 27 SNSFVGTEEYIAPEVITGAGHTSAIDWWALGILLYEMLYGRTPFRGKNRKKTFYNILHKD 206
Query: 251 LRFPDATP 274
L FP + P
Sbjct: 207 LTFPSSIP 230
Score = 72.5 bits (152), Expect(2) = 9e-038
Identities = 28/60 (46%), Positives = 38/60 (63%)
Frame = +2 / +3
Query: 278 VPVSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDPADL 457
+PVS A+ LI GLL +DP RI + GA +IK+HPFF+ + W L+R PP + P L
Sbjct: 225 IPVSLEAKQLIHGLLQRDPSSRIGSNTGANDIKEHPFFEDIYWPLIRCMSPPELDVPLKL 404
>gb|BE421430.1|BE421430 HWM009.C03 ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM009.C03, mRNA sequence
Length = 832
Score = 103 bits (220), Expect(2) = 1e-037
Identities = 37/64 (57%), Positives = 48/64 (75%)
Frame = +2 / +2
Query: 71 STSFVGTHEYLAPEIVRGEGHGSAVDWWTFGIFLYELLHGTTPFKGDSNRATLCNVVDQP 250
S SFVGT EY+APEI+ G GH SAVDWW GI LYE+L+G TPF+G + + T N++ +
Sbjct: 200 SNSFVGTEEYIAPEIITGAGHTSAVDWWALGILLYEMLYGYTPFRGKTRQRTFANILHKD 379
Query: 251 LRFP 262
+RFP
Sbjct: 380 IRFP 391
Score = 82.6 bits (174), Expect(2) = 9e-021
Identities = 34/67 (50%), Positives = 45/67 (67%)
Frame = -2 / -2
Query: 270 VASGNRSGWSTTLHSVARLLSPLNGVVPWRSSYRKMPKVHQSTALPWPSPLTISGARYSC 91
+ +GN L +V L+ PL GV P+ SYR++P HQSTAL WP+P+ ISGA YS
Sbjct: 399 ILAGNLMSLCRMLANVLCLVLPLKGV*PYSISYRRIPSAHQSTALVWPAPVIISGAMYSS 220
Query: 90 VPTNDVD 70
VPTN++D
Sbjct: 219 VPTNELD 199
Score = 72.5 bits (152), Expect(2) = 1e-037
Identities = 30/57 (52%), Positives = 37/57 (64%)
Frame = +2 / +2
Query: 278 VPVSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDP 448
+ VS AR LI LL +DP R+ + G+ EIKQHPFF G+NWALVR PP + P
Sbjct: 398 ISVSLPARQLIYRLLHRDPANRMGSYEGSNEIKQHPFFRGINWALVRGTAPPKLDAP 568
Score = 36.8 bits (74), Expect(2) = 9e-021
Identities = 16/26 (61%), Positives = 16/26 (61%)
Frame = -2 / -2
Query: 405 QLTPSKNGCCLISAAPLLVAILFWGS 328
QL P KNGCC IS P ILF GS
Sbjct: 525 QLMPRKNGCCFISFDPSYDPILFAGS 448
>gb|AV926878.1|AV926878 AV926878 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd24k23 5', mRNA sequence
Length = 703
Score = 103 bits (220), Expect(2) = 1e-037
Identities = 37/64 (57%), Positives = 48/64 (75%)
Frame = +2 / +1
Query: 71 STSFVGTHEYLAPEIVRGEGHGSAVDWWTFGIFLYELLHGTTPFKGDSNRATLCNVVDQP 250
S SFVGT EY+APEI+ G GH SAVDWW GI LYE+L+G TPF+G + + T N++ +
Sbjct: 139 SNSFVGTEEYIAPEIITGAGHTSAVDWWALGILLYEMLYGYTPFRGKTRQRTFANILHKD 318
Query: 251 LRFP 262
+RFP
Sbjct: 319 IRFP 330
Score = 82.6 bits (174), Expect(2) = 9e-021
Identities = 34/67 (50%), Positives = 45/67 (67%)
Frame = -2 / -3
Query: 270 VASGNRSGWSTTLHSVARLLSPLNGVVPWRSSYRKMPKVHQSTALPWPSPLTISGARYSC 91
+ +GN L +V L+ PL GV P+ SYR++P HQSTAL WP+P+ ISGA YS
Sbjct: 338 ILAGNLMSLCRMLANVLCLVLPLKGV*PYSISYRRIPSAHQSTALVWPAPVIISGAMYSS 159
Query: 90 VPTNDVD 70
VPTN++D
Sbjct: 158 VPTNELD 138
Score = 72.5 bits (152), Expect(2) = 1e-037
Identities = 30/57 (52%), Positives = 37/57 (64%)
Frame = +2 / +1
Query: 278 VPVSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDP 448
+ VS AR LI LL +DP R+ + G+ EIKQHPFF G+NWALVR PP + P
Sbjct: 337 ISVSLPARQLIYRLLHRDPANRMGSYEGSNEIKQHPFFRGINWALVRGTAPPKLDAP 507
Score = 36.8 bits (74), Expect(2) = 9e-021
Identities = 16/26 (61%), Positives = 16/26 (61%)
Frame = -2 / -3
Query: 405 QLTPSKNGCCLISAAPLLVAILFWGS 328
QL P KNGCC IS P ILF GS
Sbjct: 464 QLMPRKNGCCFISFDPSYDPILFAGS 387
>gb|BU997959.1|BU997959 HI09I16r HI Hordeum vulgare subsp. vulgare cDNA clone HI09I16
5-PRIME, mRNA sequence
Length = 665
Score = 103 bits (220), Expect(2) = 1e-037
Identities = 37/64 (57%), Positives = 48/64 (75%)
Frame = +2 / +2
Query: 71 STSFVGTHEYLAPEIVRGEGHGSAVDWWTFGIFLYELLHGTTPFKGDSNRATLCNVVDQP 250
S SFVGT EY+APEI+ G GH SAVDWW GI LYE+L+G TPF+G + + T N++ +
Sbjct: 155 SNSFVGTEEYIAPEIITGAGHTSAVDWWALGILLYEMLYGYTPFRGKTRQRTFANILHKD 334
Query: 251 LRFP 262
+RFP
Sbjct: 335 IRFP 346
Score = 82.6 bits (174), Expect(2) = 9e-021
Identities = 34/67 (50%), Positives = 45/67 (67%)
Frame = -2 / -3
Query: 270 VASGNRSGWSTTLHSVARLLSPLNGVVPWRSSYRKMPKVHQSTALPWPSPLTISGARYSC 91
+ +GN L +V L+ PL GV P+ SYR++P HQSTAL WP+P+ ISGA YS
Sbjct: 354 ILAGNLMSLCRMLANVLCLVLPLKGV*PYSISYRRIPSAHQSTALVWPAPVIISGAMYSS 175
Query: 90 VPTNDVD 70
VPTN++D
Sbjct: 174 VPTNELD 154
Score = 72.5 bits (152), Expect(2) = 1e-037
Identities = 30/57 (52%), Positives = 37/57 (64%)
Frame = +2 / +2
Query: 278 VPVSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDP 448
+ VS AR LI LL +DP R+ + G+ EIKQHPFF G+NWALVR PP + P
Sbjct: 353 ISVSLPARQLIYRLLHRDPANRMGSYEGSNEIKQHPFFRGINWALVRGTAPPKLDAP 523
Score = 36.8 bits (74), Expect(2) = 9e-021
Identities = 16/26 (61%), Positives = 16/26 (61%)
Frame = -2 / -3
Query: 405 QLTPSKNGCCLISAAPLLVAILFWGS 328
QL P KNGCC IS P ILF GS
Sbjct: 480 QLMPRKNGCCFISFDPSYDPILFAGS 403
>gb|BF626879.2|BF626879 HVSMEb0001J09f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0001J09f, mRNA sequence
Length = 780
Score = 103 bits (220), Expect(2) = 1e-036
Identities = 37/64 (57%), Positives = 48/64 (75%)
Frame = +2 / +1
Query: 71 STSFVGTHEYLAPEIVRGEGHGSAVDWWTFGIFLYELLHGTTPFKGDSNRATLCNVVDQP 250
S SFVGT EY+APEI+ G GH SAVDWW GI LYE+L+G TPF+G + + T N++ +
Sbjct: 199 SNSFVGTEEYIAPEIITGAGHTSAVDWWALGILLYEMLYGYTPFRGKTRQRTFANILHKD 378
Query: 251 LRFP 262
+RFP
Sbjct: 379 IRFP 390
Score = 82.6 bits (174), Expect(2) = 9e-021
Identities = 34/67 (50%), Positives = 45/67 (67%)
Frame = -2 / -2
Query: 270 VASGNRSGWSTTLHSVARLLSPLNGVVPWRSSYRKMPKVHQSTALPWPSPLTISGARYSC 91
+ +GN L +V L+ PL GV P+ SYR++P HQSTAL WP+P+ ISGA YS
Sbjct: 398 ILAGNLMSLCRMLANVLCLVLPLKGV*PYSISYRRIPSAHQSTALVWPAPVIISGAMYSS 219
Query: 90 VPTNDVD 70
VPTN++D
Sbjct: 218 VPTNELD 198
Score = 68.9 bits (144), Expect(2) = 1e-036
Identities = 29/57 (50%), Positives = 36/57 (63%)
Frame = +2 / +1
Query: 278 VPVSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDP 448
+ VS AR LI LL +DP R+ + G+ EIKQHPFF G+NWALVR P + P
Sbjct: 397 ISVSLPARQLIYRLLHRDPANRMGSYEGSNEIKQHPFFRGINWALVRGTAPSKLDAP 567
Score = 36.8 bits (74), Expect(2) = 9e-021
Identities = 16/26 (61%), Positives = 16/26 (61%)
Frame = -2 / -2
Query: 405 QLTPSKNGCCLISAAPLLVAILFWGS 328
QL P KNGCC IS P ILF GS
Sbjct: 524 QLMPRKNGCCFISFDPSYDPILFAGS 447
>gb|BJ546140.1|BJ546140 BJ546140 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak34b18 3', mRNA sequence
Length = 719
Score = 90.9 bits (192), Expect(2) = 8e-034
Identities = 30/60 (50%), Positives = 43/60 (71%)
Frame = +2 / -3
Query: 95 EYLAPEIVRGEGHGSAVDWWTFGIFLYELLHGTTPFKGDSNRATLCNVVDQPLRFPDATP 274
EY+APE++ G GH SA+DWW GI LYE+L+G TPF+G + + T N++ + L FP + P
Sbjct: 717 EYIAPEVITGAGHTSAIDWWALGILLYEMLYGRTPFRGKNRKKTFYNILHKDLTFPSSIP 538
Score = 72.5 bits (152), Expect(2) = 8e-034
Identities = 28/60 (46%), Positives = 38/60 (63%)
Frame = +2 / -3
Query: 278 VPVSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDPADL 457
+PVS A+ LI GLL +DP RI + GA +IK+HPFF+ + W L+R PP + P L
Sbjct: 543 IPVSLEAKQLIHGLLQRDPSSRIGSNTGANDIKEHPFFEDIYWPLIRCMSPPELDVPLKL 364
>gb|BF254638.2|BF254638 HVSMEf0004J12f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0004J12f, mRNA sequence
Length = 816
Score = 138 bits (296), Expect = 3e-032
Identities = 56/75 (74%), Positives = 61/75 (81%)
Frame = +2 / +1
Query: 41 EFNAEPTDARSTSFVGTHEYLAPEIVRGEGHGSAVDWWTFGIFLYELLHGTTPFKGDSNR 220
E AEPTDARS SFVGTHEYLAPEI++GEGHGSAV+WWT GIFL EL+ G T FKG NR
Sbjct: 181 ELVAEPTDARSMSFVGTHEYLAPEIIKGEGHGSAVNWWTLGIFLDELVLGETAFKGSGNR 360
Query: 221 ATLCNVVDQPLRFPD 265
A L NV QPLRFP+
Sbjct: 361 AALLNVGGQPLRFPE 405
Score = 107 bits (228), Expect = 6e-023
Identities = 47/75 (62%), Positives = 52/75 (69%)
Frame = -2 / -2
Query: 264 SGNRSGWSTTLHSVARLLSPLNGVVPWRSSYRKMPKVHQSTALPWPSPLTISGARYSCVP 85
SGN GW T +S A PLN V P SS + MPKVHQ TALP PSP ISGA+YS VP
Sbjct: 404 SGNLRGWPPTFNSAALFPEPLNAVSPKTSSSKNMPKVHQFTALPCPSPFMISGAKYSWVP 225
Query: 84 TNDVDRASVGSALNS 40
TND+D ASVGSA +S
Sbjct: 224 TNDIDLASVGSATSS 180
>gb|CB881798.1|CB881798 HM10O13w HM Hordeum vulgare subsp. vulgare cDNA clone HM10O13
3-PRIME, mRNA sequence
Length = 589
Score = 102 bits (218), Expect(2) = 2e-030
Identities = 39/60 (65%), Positives = 48/60 (80%)
Frame = +2 / -2
Query: 284 VSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDPADLGR 463
V ARDLIRGLL+K+PQ R+A +RGA EIKQHPFF+GVNWAL+R A PP +P P ++ R
Sbjct: 507 VGFAARDLIRGLLIKEPQHRLAYKRGATEIKQHPFFEGVNWALIRCATPPDIPKPVEIPR 328
Score = 49.2 bits (101), Expect(2) = 2e-030
Identities = 19/25 (76%), Positives = 21/25 (84%)
Frame = +2 / -2
Query: 194 TPFKGDSNRATLCNVVDQPLRFPDA 268
TPFKG NRATL NVV QPLRFP++
Sbjct: 588 TPFKGSGNRATLFNVVGQPLRFPES 514
>gb|BJ547950.1|BJ547950 BJ547950 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal12f06 3', mRNA sequence
Length = 671
Score = 77.1 bits (162), Expect(2) = 1e-029
Identities = 25/54 (46%), Positives = 37/54 (68%)
Frame = +2 / -1
Query: 113 IVRGEGHGSAVDWWTFGIFLYELLHGTTPFKGDSNRATLCNVVDQPLRFPDATP 274
++ G GH SA+DWW GI LYE+L+G TPF+G + + T N++ + L FP + P
Sbjct: 656 VITGAGHTSAIDWWALGILLYEMLYGRTPFRGKNRKKTFYNILHKDLTFPSSIP 495
Score = 72.5 bits (152), Expect(2) = 1e-029
Identities = 28/60 (46%), Positives = 38/60 (63%)
Frame = +2 / -1
Query: 278 VPVSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDPADL 457
+PVS A+ LI GLL +DP RI + GA +IK+HPFF+ + W L+R PP + P L
Sbjct: 500 IPVSLEAKQLIHGLLQRDPSSRIGSNTGANDIKEHPFFEDIYWPLIRCMSPPELDVPLKL 321
>gb|BJ548315.1|BJ548315 BJ548315 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal23p18 3', mRNA sequence
Length = 654
Score = 77.1 bits (162), Expect(2) = 1e-029
Identities = 25/54 (46%), Positives = 37/54 (68%)
Frame = +2 / -2
Query: 113 IVRGEGHGSAVDWWTFGIFLYELLHGTTPFKGDSNRATLCNVVDQPLRFPDATP 274
++ G GH SA+DWW GI LYE+L+G TPF+G + + T N++ + L FP + P
Sbjct: 641 VITGAGHTSAIDWWALGILLYEMLYGRTPFRGKNRKKTFYNILHKDLTFPSSIP 480
Score = 72.5 bits (152), Expect(2) = 1e-029
Identities = 28/60 (46%), Positives = 38/60 (63%)
Frame = +2 / -2
Query: 278 VPVSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDPADL 457
+PVS A+ LI GLL +DP RI + GA +IK+HPFF+ + W L+R PP + P L
Sbjct: 485 IPVSLEAKQLIHGLLQRDPSSRIGSNTGANDIKEHPFFEDIYWPLIRCMSPPELDVPLKL 306
>gb|BJ546986.1|BJ546986 BJ546986 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal23p17 5', mRNA sequence
Length = 624
Score = 77.1 bits (162), Expect(2) = 1e-029
Identities = 25/54 (46%), Positives = 37/54 (68%)
Frame = +2 / +2
Query: 113 IVRGEGHGSAVDWWTFGIFLYELLHGTTPFKGDSNRATLCNVVDQPLRFPDATP 274
++ G GH SA+DWW GI LYE+L+G TPF+G + + T N++ + L FP + P
Sbjct: 29 VITGAGHTSAIDWWALGILLYEMLYGRTPFRGKNRKKTFYNILHKDLTFPSSIP 190
Score = 72.5 bits (152), Expect(2) = 1e-029
Identities = 28/60 (46%), Positives = 38/60 (63%)
Frame = +2 / +2
Query: 278 VPVSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDPADL 457
+PVS A+ LI GLL +DP RI + GA +IK+HPFF+ + W L+R PP + P L
Sbjct: 185 IPVSLEAKQLIHGLLQRDPSSRIGSNTGANDIKEHPFFEDIYWPLIRCMSPPELDVPLKL 364
>gb|AV834352.1|AV834352 AV834352 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags15d19, mRNA sequence
Length = 619
Score = 90.4 bits (191), Expect(2) = 3e-029
Identities = 35/53 (66%), Positives = 43/53 (81%)
Frame = +2 / -2
Query: 284 VSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVP 442
VS ARDLIRGLLVK+P+ R+ +R+GAAEIK+HPFF G+NWAL+R PP P
Sbjct: 507 VSYQARDLIRGLLVKEPEHRLGSRKGAAEIKRHPFFQGLNWALIRWTAPPETP 349
Score = 57.9 bits (120), Expect(2) = 3e-029
Identities = 23/35 (65%), Positives = 27/35 (77%)
Frame = +2 / -2
Query: 164 IFLYELLHGTTPFKGDSNRATLCNVVDQPLRFPDA 268
IFLYELL+G TPF+G N TL NVV Q L+FPD+
Sbjct: 618 IFLYELLYGKTPFRGPGNDETLTNVVSQGLKFPDS 514
>gb|AJ462548.1|AJ462548 AJ462548 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200081A03F1, mRNA sequence
Length = 360
Score = 109 bits (232), Expect(2) = 1e-026
Identities = 44/90 (48%), Positives = 57/90 (63%)
Frame = +2 / +1
Query: 284 VSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDPADLGR 463
VS ARDLIRGLLVK+PQ+R+ +RGA EIKQHPFF+GVNWAL+R A PP VP P D+ R
Sbjct: 55 VSFSARDLIRGLLVKEPQQRLGCKRGATEIKQHPFFEGVNWALIRCASPPEVPKPVDIER 234
Query: 464 VASKEXXXXXXXXXXXXXXXXXXTGNFVQY 553
+K + N++++
Sbjct: 235 QTTKLPVSTSEASAAPTGASQKGSDNYLEF 324
Score = 29.9 bits (59), Expect(2) = 1e-026
Identities = 11/15 (73%), Positives = 13/15 (86%)
Frame = +2 / +1
Query: 221 ATLCNVVDQPLRFPD 265
ATL NV+ QPLRFP+
Sbjct: 1 ATLFNVIGQPLRFPE 45
>gb|BF622824.2|BF622824 HVSMEa0009C21f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0009C21f, mRNA sequence
Length = 721
Score = 95.0 bits (201), Expect(2) = 1e-026
Identities = 36/54 (66%), Positives = 43/54 (79%)
Frame = +2 / +3
Query: 287 STVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDP 448
S RDLIRGLL K+PQ+R+ +RGA EIKQHPFF+GVNWAL+R + PP VP P
Sbjct: 87 SYAGRDLIRGLLAKEPQQRLGVKRGATEIKQHPFFEGVNWALIRCSTPPEVPRP 248
Score = 44.1 bits (90), Expect(2) = 1e-026
Identities = 17/25 (68%), Positives = 20/25 (80%)
Frame = +2 / +3
Query: 194 TPFKGDSNRATLCNVVDQPLRFPDA 268
TPFKG NRATL NVV Q L+FP++
Sbjct: 3 TPFKGSGNRATLFNVVGQQLKFPES 77
>gb|BG415060.1|BG415060 HVSMEk0004P24f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0004P24f, mRNA sequence
Length = 632
Score = 102 bits (218), Expect(2) = 2e-026
Identities = 39/60 (65%), Positives = 48/60 (80%)
Frame = +2 / +3
Query: 284 VSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDPADLGR 463
V ARDLIRGLL+K+PQ R+A +RGA EIKQHPFF+GVNWAL+R A PP +P P ++ R
Sbjct: 69 VGFAARDLIRGLLIKEPQHRLAYKRGATEIKQHPFFEGVNWALIRCATPPDIPKPVEIPR 248
Score = 35.9 bits (72), Expect(2) = 2e-026
Identities = 14/18 (77%), Positives = 16/18 (88%)
Frame = +2 / +3
Query: 215 NRATLCNVVDQPLRFPDA 268
NRATL NVV QPLRFP++
Sbjct: 9 NRATLFNVVGQPLRFPES 62
>gb|BJ546987.1|BJ546987 BJ546987 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal23p18 5', mRNA sequence
Length = 441
Score = 72.5 bits (152), Expect(2) = 6e-025
Identities = 28/60 (46%), Positives = 38/60 (63%)
Frame = +2 / +1
Query: 278 VPVSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDPADL 457
+PVS A+ LI GLL +DP RI + GA +IK+HPFF+ + W L+R PP + P L
Sbjct: 160 IPVSLEAKQLIHGLLQRDPSSRIGSNTGANDIKEHPFFEDIYWPLIRCMSPPELDVPLKL 339
Score = 61.1 bits (127), Expect(2) = 6e-025
Identities = 20/43 (46%), Positives = 29/43 (67%)
Frame = +2 / +1
Query: 146 DWWTFGIFLYELLHGTTPFKGDSNRATLCNVVDQPLRFPDATP 274
DWW GI LYE+L+G TPF+G + + T N++ + L FP + P
Sbjct: 37 DWWALGILLYEMLYGRTPFRGKNRKKTFYNMLHKDLTFPSSIP 165
>gb|AV919193.1|AV919193 AV919193 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags4o22 3', mRNA sequence
Length = 586
Score = 72.5 bits (152), Expect(2) = 8e-025
Identities = 30/57 (52%), Positives = 37/57 (64%)
Frame = +2 / -3
Query: 278 VPVSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDP 448
+ VS AR LI LL +DP R+ + G+ EIKQHPFF G+NWALVR PP + P
Sbjct: 461 ISVSLPARQLIYRLLHRDPANRMGSYEGSNEIKQHPFFRGINWALVRGTAPPKLDAP 291
Score = 60.6 bits (126), Expect(2) = 8e-025
Identities = 19/39 (48%), Positives = 28/39 (71%)
Frame = +2 / -3
Query: 146 DWWTFGIFLYELLHGTTPFKGDSNRATLCNVVDQPLRFP 262
DWW GI LYE+L+G TPF+G + + T N++ + +RFP
Sbjct: 584 DWWALGILLYEMLYGYTPFRGKTRQRTFANILHKDIRFP 468
>gb|CB875785.1|CB875785 HX09F12w HX Hordeum vulgare subsp. vulgare cDNA clone HX09F12
3-PRIME, mRNA sequence
Length = 660
Score = 103 bits (219), Expect(2) = 7e-024
Identities = 40/58 (68%), Positives = 47/58 (81%)
Frame = +2 / -1
Query: 284 VSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDPADL 457
VS ARD+IRGLLVKDPQ R+ +RGA EIKQHPFF+GVNWAL+R A PP +P P +L
Sbjct: 612 VSFSARDMIRGLLVKDPQHRLGHKRGATEIKQHPFFEGVNWALIRCASPPDIPKPVEL 439
Score = 26.7 bits (52), Expect(2) = 7e-024
Identities = 10/14 (71%), Positives = 12/14 (85%)
Frame = +2 / -1
Query: 227 LCNVVDQPLRFPDA 268
L NVV QPLRFP++
Sbjct: 660 LFNVVGQPLRFPES 619
>gb|BJ547677.1|BJ547677 BJ547677 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal34l02 5', mRNA sequence
Length = 616
Score = 72.5 bits (152), Expect(2) = 2e-021
Identities = 28/60 (46%), Positives = 38/60 (63%)
Frame = +2 / +1
Query: 278 VPVSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDPADL 457
+PVS A+ LI GLL +DP RI + GA +IK+HPFF+ + W L+R PP + P L
Sbjct: 172 IPVSLEAKQLIHGLLQRDPSSRIGSNTGANDIKEHPFFEDIYWPLIRCMSPPELDVPLKL 351
Score = 49.2 bits (101), Expect(2) = 2e-021
Identities = 17/38 (44%), Positives = 26/38 (68%)
Frame = +2 / +1
Query: 161 GIFLYELLHGTTPFKGDSNRATLCNVVDQPLRFPDATP 274
GI LYE+L+G TPF+G + + T N++ + L FP + P
Sbjct: 64 GILLYEMLYGRTPFRGKNRKKTFYNILHKDLTFPSSIP 177
>gb|AV832731.1|AV832731 AV832731 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare top three leaves adult, heading stage Hordeum
vulgare subsp. vulgare cDNA clone baal3j22, mRNA
sequence
Length = 476
Score = 72.5 bits (152), Expect(2) = 2e-021
Identities = 28/60 (46%), Positives = 38/60 (63%)
Frame = +2 / +3
Query: 278 VPVSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDPADL 457
+PVS A+ LI GLL +DP RI + GA +IK+HPFF+ + W L+R PP + P L
Sbjct: 168 IPVSLEAKQLIHGLLQRDPSSRIGSNTGANDIKEHPFFEDIYWPLIRCMSPPELDVPLKL 347
Score = 49.2 bits (101), Expect(2) = 2e-021
Identities = 17/38 (44%), Positives = 26/38 (68%)
Frame = +2 / +3
Query: 161 GIFLYELLHGTTPFKGDSNRATLCNVVDQPLRFPDATP 274
GI LYE+L+G TPF+G + + T N++ + L FP + P
Sbjct: 60 GILLYEMLYGRTPFRGKNRKKTFYNILHKDLTFPSSIP 173
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 299,695,826
Number of Sequences: 312970
Number of extensions: 5900464
Number of successful extensions: 95671
Number of sequences better than 1.0e-020: 38
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6659423313
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)