TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4424417.2.1
(1137 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ545538.1|BJ545538 BJ545538 K. Sato unpublished cDNA li... 290 1e-077
gb|AV836836.1|AV836836 AV836836 K. Sato unpublished cDNA li... 286 1e-076
gb|BJ544020.1|BJ544020 BJ544020 K. Sato unpublished cDNA li... 281 7e-075
gb|CB881999.1|CB881999 HM11I22w HM Hordeum vulgare subsp. v... 262 3e-069
gb|BJ550107.1|BJ550107 BJ550107 K. Sato unpublished cDNA li... 253 1e-066
gb|BU978527.1|BU978527 HA13H23r HA Hordeum vulgare subsp. v... 235 4e-061
gb|BQ753310.1|BQ753310 EBan01_SQ001_I10_R anther, yellow st... 228 5e-059
gb|BJ549163.1|BJ549163 BJ549163 K. Sato unpublished cDNA li... 226 2e-058
gb|BJ447263.1|BJ447263 BJ447263 K. Sato unpublished cDNA li... 222 2e-057
gb|BJ454765.1|BJ454765 BJ454765 K. Sato unpublished cDNA li... 222 2e-057
gb|BQ759606.1|BQ759606 EBpi03_SQ002_D17_R pistil, 4 DPA, no... 221 6e-057
gb|BU977917.1|BU977917 HA12E09r HA Hordeum vulgare subsp. v... 201 1e-054
gb|AV916085.1|AV916085 AV916085 K. Sato unpublished cDNA li... 211 5e-054
gb|BJ548462.1|BJ548462 BJ548462 K. Sato unpublished cDNA li... 138 5e-054
gb|BF257150.3|BF257150 HVSMEf0012A06f Hordeum vulgare seedl... 206 2e-052
gb|BU977918.1|BU977918 HA12E09u HA Hordeum vulgare subsp. v... 198 5e-050
gb|CB879610.1|CB879610 HP02P10w HP Hordeum vulgare subsp. v... 182 2e-045
gb|CA001190.1|CA001190 HS05A02u HS Hordeum vulgare subsp. v... 176 3e-043
gb|CB880789.1|CB880789 HM07N11w HM Hordeum vulgare subsp. v... 164 1e-039
gb|BU994127.1|BU994127 HM05N11r HM Hordeum vulgare subsp. v... 162 4e-039
gb|BG310264.2|BG310264 HVSMEc0016L11f Hordeum vulgare seedl... 160 1e-038
gb|BQ660971.1|BQ660971 HM01F04w HM Hordeum vulgare subsp. v... 157 9e-038
gb|CB881341.1|CB881341 HM09I05w HM Hordeum vulgare subsp. v... 157 9e-038
gb|AV917273.1|AV917273 AV917273 K. Sato unpublished cDNA li... 155 4e-037
gb|CB858813.1|CB858813 HI08G13w HI Hordeum vulgare subsp. v... 153 2e-036
gb|BJ456704.1|BJ456704 BJ456704 K. Sato unpublished cDNA li... 96 1e-035
gb|BU999880.1|BU999880 HS17B20u HS Hordeum vulgare subsp. v... 96 1e-035
gb|BI957127.1|BI957127 HVSMEn0007J20f Hordeum vulgare rachi... 149 3e-035
gb|AV931399.1|AV931399 AV931399 K. Sato unpublished cDNA li... 95 4e-035
gb|AV922443.1|AV922443 AV922443 K. Sato unpublished cDNA li... 148 7e-035
gb|BF621349.2|BF621349 HVSMEa0006J13f Hordeum vulgare seedl... 112 6e-034
gb|BQ458310.1|BQ458310 HA05J23r HA Hordeum vulgare subsp. v... 132 6e-034
gb|BF254987.2|BF254987 HVSMEf0005J24f Hordeum vulgare seedl... 143 2e-033
gb|BI957446.1|BI957446 HVSMEn0009J16f Hordeum vulgare rachi... 140 2e-033
emb|X92754.1|HVCADMRNA H.vulgare mRNA for cinnamyl alcohol ... 140 2e-032
gb|CB881629.1|CB881629 HM10G11w HM Hordeum vulgare subsp. v... 139 2e-032
gb|AL509755.1|AL509755 AL509755 Hordeum vulgare Barke devel... 96 6e-031
gb|BQ661428.1|BQ661428 HM03H06u HM Hordeum vulgare subsp. v... 132 5e-030
gb|BI947283.1|BI947283 HVSMEl0005A06f Hordeum vulgare spike... 100 6e-030
gb|CB878272.1|CB878272 HP07O19T HP Hordeum vulgare subsp. v... 131 9e-030
gb|BJ455039.1|BJ455039 BJ455039 K. Sato unpublished cDNA li... 96 3e-028
gb|BI960471.1|BI960471 HVSMEn0024L10f Hordeum vulgare rachi... 124 1e-027
gb|BQ761920.1|BQ761920 EBpi01_SQ005_D08_R pistil, 1 DPA, no... 124 1e-027
gb|CB880767.1|CB880767 HM07M03w HM Hordeum vulgare subsp. v... 124 1e-027
gb|BI957762.1|BI957762 HVSMEn0011B06f Hordeum vulgare rachi... 117 3e-027
gb|BQ768475.1|BQ768475 EBro08_SQ011_G19_R root, 3 week, dro... 85 4e-027
gb|BI949722.1|BI949722 HVSMEl0015I24f Hordeum vulgare spike... 122 5e-027
gb|BQ662843.1|BQ662843 HS04G20u HS Hordeum vulgare subsp. v... 96 1e-026
gb|BM368384.2|BM368384 EBed01_SQ002_P23_R endosperm, 6 DPA,... 119 2e-026
gb|BG299988.1|BG299988 HVSMEa0022N15f Hordeum vulgare seedl... 114 8e-025
gb|BF255211.2|BF255211 HVSMEf0006E02f Hordeum vulgare seedl... 106 2e-022
gb|BQ661689.1|BQ661689 HM04O09u HM Hordeum vulgare subsp. v... 106 2e-022
gb|BQ661200.1|BQ661200 HM02G09u HM Hordeum vulgare subsp. v... 96 8e-022
gb|AL510922.1|AL510922 AL510922 Hordeum vulgare Barke devel... 103 2e-021
gb|BE194442.1|BE194442 HVSMEh0085I24f Hordeum vulgare 5-45 ... 100 3e-021
>gb|BJ545538.1|BJ545538 BJ545538 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak17o14 3', mRNA sequence
Length = 634
Score = 290 bits (627), Expect = 1e-077
Identities = 120/145 (82%), Positives = 137/145 (94%)
Frame = +2 / -2
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
IS+SPGKKQEAL+RLGADAFVVSKDA+EMKAA STMDGIINTVSAN+ +AP +GLLKPNG
Sbjct: 624 ISSSPGKKQEALERLGADAFVVSKDADEMKAAVSTMDGIINTVSANIPMAPLLGLLKPNG 445
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADY 481
KMIMVGLP +P+E+PPF L+ NKTLAGSCIGGMRDTQEM+++AAKHGVTADIE+++A+Y
Sbjct: 444 KMIMVGLPEQPIEVPPFALVARNKTLAGSCIGGMRDTQEMLDLAAKHGVTADIEVISAEY 265
Query: 482 VNTAMERLAKADVRYRFVIDIGNTL 556
VNTAMERLAKADVRYRFVIDI NTL
Sbjct: 264 VNTAMERLAKADVRYRFVIDIANTL 190
Score = 186 bits (400), Expect = 2e-046
Identities = 84/136 (61%), Positives = 96/136 (70%)
Frame = -3 / +1
Query: 562 ILEGXXXXXXXXXXXVSLGQALHGRVHVVRGHQFYVRRHAVLGRHVDHLLRVPHAADAAP 383
++EG VSLG+ALH VHV+R + V RHAVL R V HLL VPHAADAAP
Sbjct: 184 LVEGVGDVDDESIPDVSLGKALHRCVHVLRAYHLDVCRHAVLRREVQHLLGVPHAADAAP 363
Query: 382 GQGLVPNDEIKGRDLERLGGQADHDHLAVGLEQAHVRGQGEVRRHRVDDAVHGARSRLHF 203
GQGLVP ++ KGRDL+RL +ADHDHLAVGLE RG G+V RHRV DA+HGA LH
Sbjct: 364 GQGLVPGNQSKGRDLDRLLWEADHDHLAVGLE*PEERGHGDVCRHRVYDAIHGAHRSLHL 543
Query: 202 FGVLADDEGVGAKPVE 155
GVLADDE V AKP+E
Sbjct: 544 VGVLADDERVSAKPLE 591
Score = 169 bits (363), Expect = 3e-041
Identities = 83/145 (57%), Positives = 85/145 (58%)
Frame = -1 / +2
Query: 555 RVLPMSMTKRYLTSALARRXXXXXXXXXXXXXXXXXTPCLAATLIISCVSLMPPMQLPAR 376
RVL MSMT RYLTSALARR TPC AA IS VSLMPPMQLPAR
Sbjct: 191 RVLAMSMTNRYLTSALARRSIAVFTYSALITSMSAVTPCFAARSSISWVSLMPPMQLPAR 370
Query: 375 VLFPMMRSKGGISRGLVGRPTMIILPLGLSRPMYGAKVRFADTVLMMPSMVXXXXXXXXX 196
VLF R+KGG S G GRPTMIILPLGLS P GA FADTV MMPSMV
Sbjct: 371 VLFLATRAKGGTSIGCSGRPTMIILPLGLSSPRRGAMGMFADTVFMMPSMVLTAAFISSA 550
Query: 195 XXXXXXXXXXSRSSASCFFPGDVLM 121
S S ASCFFPGD L+
Sbjct: 551 SLLTTNASAPSLSRASCFFPGDELI 625
Score = 101 bits (215), Expect(2) = 2e-028
Identities = 42/64 (65%), Positives = 45/64 (70%)
Frame = +1 / -1
Query: 220 EHHGRHHQHGVGEPHLGPVHGPAQAQRQDDHGRPAHQASRDPAL*SHHWEQDPGRELHRR 399
EHHG HH+HGV + GP QAQRQDDHGRP A R P+L S EQDPGRELHRR
Sbjct: 526 EHHGWHHKHGVCKHPHGPSPRATQAQRQDDHGRPPRAAYRGPSLCSGCQEQDPGRELHRR 347
Query: 400 HEGH 411
HEGH
Sbjct: 346 HEGH 335
Score = 44.6 bits (91), Expect(2) = 2e-028
Identities = 17/23 (73%), Positives = 20/23 (86%)
Frame = +1 / -1
Query: 499 APGQG*RQVPLRHRHRQHPQEFR 567
AP QG*RQV +RHRHRQHP++ R
Sbjct: 247 APCQG*RQVSIRHRHRQHPRQGR 179
>gb|AV836836.1|AV836836 AV836836 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd27e20, mRNA
sequence
Length = 646
Score = 286 bits (620), Expect = 1e-076
Identities = 121/148 (81%), Positives = 136/148 (91%)
Frame = +2 / -2
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
IS+SPGKKQEAL+RLGADAFVVSKDAEEMKAA +TMDGIINTVSAN+ +AP +GLLKPNG
Sbjct: 630 ISSSPGKKQEALERLGADAFVVSKDAEEMKAAMNTMDGIINTVSANIPMAPLLGLLKPNG 451
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADY 481
KMIMVGLP P+EI PF L+ NKTLAGSCIGGMRDTQEM+++AAKH VTADIE++ A+Y
Sbjct: 450 KMIMVGLPENPMEISPFALVATNKTLAGSCIGGMRDTQEMLDLAAKHDVTADIEVIDAEY 271
Query: 482 VNTAMERLAKADVRYRFVIDIGNTLKNS 565
VNTAMERLAKADVRYRFVIDIGNTL N+
Sbjct: 270 VNTAMERLAKADVRYRFVIDIGNTLDNA 187
Score = 166 bits (358), Expect = 2e-040
Identities = 81/145 (55%), Positives = 84/145 (57%)
Frame = -1 / +2
Query: 555 RVLPMSMTKRYLTSALARRXXXXXXXXXXXXXXXXXTPCLAATLIISCVSLMPPMQLPAR 376
RVLPMSMT RYLTSALA R T C AA IS VSLMPPMQLPAR
Sbjct: 197 RVLPMSMTNRYLTSALASRSMAVFTYSASITSMSAVTSCFAARSSISWVSLMPPMQLPAR 376
Query: 375 VLFPMMRSKGGISRGLVGRPTMIILPLGLSRPMYGAKVRFADTVLMMPSMVXXXXXXXXX 196
VLF R+KG IS G GRPTMIILPLG S P GA FADTV MMPSMV
Sbjct: 377 VLFVATRAKGEISIGFSGRPTMIILPLGFSSPKRGAMGMFADTVFMMPSMVFIAAFISSA 556
Query: 195 XXXXXXXXXXSRSSASCFFPGDVLM 121
+RS ASCFFPGD L+
Sbjct: 557 SLLTTNASAPNRSRASCFFPGDELI 631
Score = 162 bits (348), Expect = 4e-039
Identities = 69/119 (57%), Positives = 85/119 (71%)
Frame = -3 / +1
Query: 511 LGQALHGRVHVVRGHQFYVRRHAVLGRHVDHLLRVPHAADAAPGQGLVPNDEIKGRDLER 332
LG++LHGRVHV+ + VRRH VL R + HLL VPHAADAAPGQGLV ++ KG DL R
Sbjct: 241 LGKSLHGRVHVLGVNHLDVRRHVVLRRQIQHLLGVPHAADAAPGQGLVRGNQSKGGDLHR 420
Query: 331 LGGQADHDHLAVGLEQAHVRGQGEVRRHRVDDAVHGARSRLHFFGVLADDEGVGAKPVE 155
+ G+A+HDHLAVG + RG G+V RHRV DA+HG LH +LAD+E VG P+E
Sbjct: 421 ILGEANHDHLAVGFQ*P*ERGHGDVCRHRVYDAIHGIHCSLHLLSILADNERVGT*PLE 597
>gb|BJ544020.1|BJ544020 BJ544020 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak17o14 5', mRNA sequence
Length = 572
Score = 281 bits (607), Expect = 7e-075
Identities = 116/140 (82%), Positives = 132/140 (94%)
Frame = +2 / +3
Query: 137 GKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNGKMIMV 316
GKKQEAL+RLGADAFVVSKDA+EMKAA STMDGIINTVSAN+ +AP +GLLKPNGKMIMV
Sbjct: 12 GKKQEALERLGADAFVVSKDADEMKAAVSTMDGIINTVSANIPMAPLLGLLKPNGKMIMV 191
Query: 317 GLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAM 496
GLP +P+E+PPF L+ NKTLAGSCIGGMRDTQEM+++AAKHGVTADIE+++A+YVNTAM
Sbjct: 192 GLPEQPIEVPPFALVARNKTLAGSCIGGMRDTQEMLDLAAKHGVTADIEVISAEYVNTAM 371
Query: 497 ERLAKADVRYRFVIDIGNTL 556
ERLAKADVRYRFVIDI NTL
Sbjct: 372 ERLAKADVRYRFVIDIANTL 431
Score = 186 bits (400), Expect = 2e-046
Identities = 84/136 (61%), Positives = 96/136 (70%)
Frame = -3 / -1
Query: 562 ILEGXXXXXXXXXXXVSLGQALHGRVHVVRGHQFYVRRHAVLGRHVDHLLRVPHAADAAP 383
++EG VSLG+ALH VHV+R + V RHAVL R V HLL VPHAADAAP
Sbjct: 437 LVEGVGDVDDESIPDVSLGKALHRCVHVLRAYHLDVCRHAVLRREVQHLLGVPHAADAAP 258
Query: 382 GQGLVPNDEIKGRDLERLGGQADHDHLAVGLEQAHVRGQGEVRRHRVDDAVHGARSRLHF 203
GQGLVP ++ KGRDL+RL +ADHDHLAVGLE RG G+V RHRV DA+HGA LH
Sbjct: 257 GQGLVPGNQSKGRDLDRLLWEADHDHLAVGLE*PEERGHGDVCRHRVYDAIHGAHRSLHL 78
Query: 202 FGVLADDEGVGAKPVE 155
GVLADDE V AKP+E
Sbjct: 77 VGVLADDERVSAKPLE 30
Score = 159 bits (341), Expect = 3e-038
Identities = 79/140 (56%), Positives = 80/140 (57%)
Frame = -1 / -2
Query: 555 RVLPMSMTKRYLTSALARRXXXXXXXXXXXXXXXXXTPCLAATLIISCVSLMPPMQLPAR 376
RVL MSMT RYLTSALARR TPC AA IS VSLMPPMQLPAR
Sbjct: 430 RVLAMSMTNRYLTSALARRSIAVFTYSALITSMSAVTPCFAARSSISWVSLMPPMQLPAR 251
Query: 375 VLFPMMRSKGGISRGLVGRPTMIILPLGLSRPMYGAKVRFADTVLMMPSMVXXXXXXXXX 196
VLF R+KGG S G GRPTMIILPLGLS P GA FADTV MMPSMV
Sbjct: 250 VLFLATRAKGGTSIGCSGRPTMIILPLGLSSPRRGAMGMFADTVFMMPSMVLTAAFISSA 71
Query: 195 XXXXXXXXXXSRSSASCFFP 136
S S ASCF P
Sbjct: 70 SLLTTNASAPSLSRASCFXP 11
Score = 101 bits (215), Expect(2) = 2e-028
Identities = 42/64 (65%), Positives = 45/64 (70%)
Frame = +1 / +2
Query: 220 EHHGRHHQHGVGEPHLGPVHGPAQAQRQDDHGRPAHQASRDPAL*SHHWEQDPGRELHRR 399
EHHG HH+HGV + GP QAQRQDDHGRP A R P+L S EQDPGRELHRR
Sbjct: 95 EHHGWHHKHGVCKHPHGPSPRATQAQRQDDHGRPPRAAYRGPSLCSGCQEQDPGRELHRR 274
Query: 400 HEGH 411
HEGH
Sbjct: 275 HEGH 286
Score = 44.6 bits (91), Expect(2) = 2e-028
Identities = 17/23 (73%), Positives = 20/23 (86%)
Frame = +1 / +2
Query: 499 APGQG*RQVPLRHRHRQHPQEFR 567
AP QG*RQV +RHRHRQHP++ R
Sbjct: 374 APCQG*RQVSIRHRHRQHPRQGR 442
>gb|CB881999.1|CB881999 HM11I22w HM Hordeum vulgare subsp. vulgare cDNA clone HM11I22
3-PRIME, mRNA sequence
Length = 596
Score = 262 bits (566), Expect = 3e-069
Identities = 108/133 (81%), Positives = 124/133 (93%)
Frame = +2 / -2
Query: 158 DRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNGKMIMVGLPTKPL 337
+RLGADAFVVSKDA+EMKAA STMDGIINTVSA + +AP +GLLKPNGKMIMVGLP +P+
Sbjct: 595 ERLGADAFVVSKDADEMKAAVSTMDGIINTVSAXIPMAPLLGLLKPNGKMIMVGLPEQPI 416
Query: 338 EIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAMERLAKAD 517
E+PPF L+ NKTLAGSCIGGMRDTQEM+++AAKHGVTADIE+++A+YVNTAMERLAKAD
Sbjct: 415 EVPPFALVARNKTLAGSCIGGMRDTQEMLDLAAKHGVTADIEVISAEYVNTAMERLAKAD 236
Query: 518 VRYRFVIDIGNTL 556
VRYRFVIDI NTL
Sbjct: 235 VRYRFVIDIANTL 197
Score = 183 bits (395), Expect = 1e-045
Identities = 83/135 (61%), Positives = 95/135 (70%)
Frame = -3 / +2
Query: 562 ILEGXXXXXXXXXXXVSLGQALHGRVHVVRGHQFYVRRHAVLGRHVDHLLRVPHAADAAP 383
++EG VSLG+ALH VHV+R + V RHAVL R V HLL VPHAADAAP
Sbjct: 191 LVEGVGDVDDESIPDVSLGKALHRCVHVLRAYHLDVCRHAVLRREVQHLLGVPHAADAAP 370
Query: 382 GQGLVPNDEIKGRDLERLGGQADHDHLAVGLEQAHVRGQGEVRRHRVDDAVHGARSRLHF 203
GQGLVP ++ KGRDL+RL +ADHDHLAVGLE RG G+V RHRV DA+HGA LH
Sbjct: 371 GQGLVPGNQSKGRDLDRLLWEADHDHLAVGLE*PEERGHGDVCRHRVYDAIHGAHRSLHL 550
Query: 202 FGVLADDEGVGAKPV 158
GVLADDE V AKP+
Sbjct: 551 VGVLADDERVSAKPL 595
Score = 145 bits (312), Expect = 3e-034
Identities = 71/111 (63%), Positives = 72/111 (64%)
Frame = -1 / +3
Query: 555 RVLPMSMTKRYLTSALARRXXXXXXXXXXXXXXXXXTPCLAATLIISCVSLMPPMQLPAR 376
RVL MSMT RYLTSALARR TPC AA IS VSLMPPMQLPAR
Sbjct: 198 RVLAMSMTNRYLTSALARRSIAVFTYSALITSMSAVTPCFAARSSISWVSLMPPMQLPAR 377
Query: 375 VLFPMMRSKGGISRGLVGRPTMIILPLGLSRPMYGAKVRFADTVLMMPSMV 223
VLF R+KGG S G GRPTMIILPLGLS P GA ADTV MMPSMV
Sbjct: 378 VLFLATRAKGGTSIGCSGRPTMIILPLGLSSPRRGAMGMXADTVFMMPSMV 530
Score = 100 bits (213), Expect(2) = 5e-028
Identities = 42/64 (65%), Positives = 44/64 (68%)
Frame = +1 / -1
Query: 220 EHHGRHHQHGVGEPHLGPVHGPAQAQRQDDHGRPAHQASRDPAL*SHHWEQDPGRELHRR 399
EHHG HH+HGV GP QAQRQDDHGRP A R P+L S EQDPGRELHRR
Sbjct: 533 EHHGWHHKHGVCXHPHGPSPRATQAQRQDDHGRPPRAAYRGPSLCSGCQEQDPGRELHRR 354
Query: 400 HEGH 411
HEGH
Sbjct: 353 HEGH 342
Score = 44.6 bits (91), Expect(2) = 5e-028
Identities = 17/23 (73%), Positives = 20/23 (86%)
Frame = +1 / -1
Query: 499 APGQG*RQVPLRHRHRQHPQEFR 567
AP QG*RQV +RHRHRQHP++ R
Sbjct: 254 APCQG*RQVSIRHRHRQHPRQGR 186
>gb|BJ550107.1|BJ550107 BJ550107 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags37c03 3', mRNA sequence
Length = 641
Score = 253 bits (547), Expect = 1e-066
Identities = 104/138 (75%), Positives = 124/138 (89%)
Frame = +2 / -2
Query: 152 ALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNGKMIMVGLPTK 331
AL RLGADAFVVS +A+EMKAA STMDGIINTVSAN+ +AP +GLLKPNGKM+MVGLP
Sbjct: 640 ALGRLGADAFVVSNNADEMKAAMSTMDGIINTVSANMPIAPLLGLLKPNGKMVMVGLPEN 461
Query: 332 PLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAMERLAK 511
+++PPF L+ NKTLAGSC+GGM +TQEM+++AAKHGVTADIE++ ADYVNTAM+RLAK
Sbjct: 460 SIQLPPFALVATNKTLAGSCMGGMSETQEMLDLAAKHGVTADIEVIRADYVNTAMDRLAK 281
Query: 512 ADVRYRFVIDIGNTLKNS 565
ADVRYRFVIDIGNTL+ +
Sbjct: 280 ADVRYRFVIDIGNTLQKA 227
Score = 169 bits (364), Expect = 2e-041
Identities = 76/134 (56%), Positives = 91/134 (67%)
Frame = -3 / +2
Query: 562 ILEGXXXXXXXXXXXVSLGQALHGRVHVVRGHQFYVRRHAVLGRHVDHLLRVPHAADAAP 383
+LEG +SLG+A+HGRV +V V RHAVLGR V HLL + HAA AAP
Sbjct: 230 LLEGVADVDDEAIPYISLGEAVHGRVDIVGTDDLDVGRHAVLGREVQHLLGLAHAAHAAP 409
Query: 382 GQGLVPNDEIKGRDLERLGGQADHDHLAVGLEQAHVRGQGEVRRHRVDDAVHGARSRLHF 203
QGLV +E +GR+L+ + +ADHDHLAVGLEQA RG G V RH VDDA+HGA LH
Sbjct: 410 RQGLVRGNESEGRELDGILREADHDHLAVGLEQAEERGYGHVCRHGVDDAIHGAHRGLHL 589
Query: 202 FGVLADDEGVGAKP 161
GV+ADDE VGA+P
Sbjct: 590 VGVVADDERVGAEP 631
Score = 152 bits (327), Expect = 3e-036
Identities = 74/113 (65%), Positives = 76/113 (67%)
Frame = -1 / +3
Query: 561 FLRVLPMSMTKRYLTSALARRXXXXXXXXXXXXXXXXXTPCLAATLIISCVSLMPPMQLP 382
F RVLPMSMTKRYLTSALARR TPCLAA IS VSLMPPMQLP
Sbjct: 231 FWRVLPMSMTKRYLTSALARRSMAVLT*SARMTSMSAVTPCLAARSSISWVSLMPPMQLP 410
Query: 381 ARVLFPMMRSKGGISRGLVGRPTMIILPLGLSRPMYGAKVRFADTVLMMPSMV 223
ARVLF R+KGG GRPTM ILPLGLS+P GA FADTVLMMPSMV
Sbjct: 411 ARVLFVATRAKGGSWMEFSGRPTMTILPLGLSKPRRGAMGMFADTVLMMPSMV 569
>gb|BU978527.1|BU978527 HA13H23r HA Hordeum vulgare subsp. vulgare cDNA clone HA13H23
5-PRIME, mRNA sequence
Length = 476
Score = 235 bits (507), Expect = 4e-061
Identities = 98/146 (67%), Positives = 120/146 (82%)
Frame = +2 / +1
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
ISTSPGK++EAL+RLGADAFV+S DA EMKAA TM GIINT SA +L PY+ LLKP+G
Sbjct: 31 ISTSPGKREEALERLGADAFVLSTDAAEMKAAVGTMHGIINTASAAASLHPYLALLKPHG 210
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADY 481
KMI++G+P KPL++ F LI G KTLAGSC+ + +TQEM++ AA+HGV AD+E+V A
Sbjct: 211 KMILLGIPNKPLQVSAFALIGGGKTLAGSCMASISETQEMLDFAAEHGVAADVEVVGAGE 390
Query: 482 VNTAMERLAKADVRYRFVIDIGNTLK 559
VN AMERLAK DVRYRFVID+GNT+K
Sbjct: 391 VNKAMERLAKGDVRYRFVIDVGNTIK 468
Score = 146 bits (313), Expect = 2e-034
Identities = 75/146 (51%), Positives = 80/146 (54%)
Frame = -1 / -1
Query: 558 LRVLPMSMTKRYLTSALARRXXXXXXXXXXXXXXXXXTPCLAATLIISCVSLMPPMQLPA 379
L VLP SMTKRYLTS LARR TPC AA ISCVSLM MQLPA
Sbjct: 467 LMVLPTSMTKRYLTSPLARRSIALFTSPAPTTSTSAATPCSAAKSSISCVSLMLAMQLPA 288
Query: 378 RVLFPMMRSKGGISRGLVGRPTMIILPLGLSRPMYGAKVRFADTVLMMPSMVXXXXXXXX 199
RVL P MR+ RGL+G P+ IILP GLS YG + AD VLMMP MV
Sbjct: 287 RVLPPPMRANAETCRGLLGMPSRIILPWGLSSARYGCRDAAADAVLMMPCMVPTAAFISA 108
Query: 198 XXXXXXXXXXXSRSSASCFFPGDVLM 121
SRSSAS FPGDVL+
Sbjct: 107 ASVLRTNASAPSRSSASSRFPGDVLI 30
Score = 131 bits (280), Expect = 9e-030
Identities = 65/135 (48%), Positives = 79/135 (58%)
Frame = -3 / -3
Query: 559 LEGXXXXXXXXXXXVSLGQALHGRVHVVRGHQFYVRRHAVLGRHVDHLLRVPHAADAAPG 380
L+G V+LGQALH VH+ +VR HA+L V+HLLR+ A AAPG
Sbjct: 468 LDGVTDVDDEAVPDVALGQALHCLVHLXGADYLHVRGHAMLCGEVEHLLRLADARHAAPG 289
Query: 379 QGLVPNDEIKGRDLERLGGQADHDHLAVGLEQAHVRGQGEVRRHRVDDAVHGARSRLHFF 200
Q L DE + DL R G A+ DHLAVG +Q VR QG VDDAVHGA LH
Sbjct: 288 QSLAAADESECGDL*RFIGDAEQDHLAVGFKQRQVRVQGRGGGRSVDDAVHGAHGGLHLR 109
Query: 199 GVLADDEGVGAKPVE 155
GV A+DE VGA+P++
Sbjct: 108 GVCAEDERVGAQPLQ 64
>gb|BQ753310.1|BQ753310 EBan01_SQ001_I10_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ001_I10 5', mRNA sequence
Length = 509
Score = 228 bits (492), Expect = 5e-059
Identities = 94/118 (79%), Positives = 108/118 (91%)
Frame = +2 / +2
Query: 203 EMKAAASTMDGIINTVSANLTLAPYMGLLKPNGKMIMVGLPTKPLEIPPFDLIIGNKTLA 382
EMKAA STMDGIINTVSAN+ +AP GLLKPNGKM+MVGLP KP+E+PPF L+ NKTL+
Sbjct: 2 EMKAAMSTMDGIINTVSANIPMAPLFGLLKPNGKMVMVGLPEKPVEVPPFALVAKNKTLS 181
Query: 383 GSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAMERLAKADVRYRFVIDIGNTL 556
GS IGGM DTQEM+++AAKHGVTADIE+++A+YVNTAMERLAKADVRYRFVIDIGNTL
Sbjct: 182 GSFIGGMSDTQEMLDLAAKHGVTADIEVISAEYVNTAMERLAKADVRYRFVIDIGNTL 355
Score = 147 bits (315), Expect = 1e-034
Identities = 70/111 (63%), Positives = 72/111 (64%)
Frame = -1 / -3
Query: 555 RVLPMSMTKRYLTSALARRXXXXXXXXXXXXXXXXXTPCLAATLIISCVSLMPPMQLPAR 376
RVLPMSMT RYLTSALARR TPC AA IS VSLMPPM+LP R
Sbjct: 354 RVLPMSMTNRYLTSALARRSIAVFTYSALITSMSAVTPCFAARSSISWVSLMPPMKLPDR 175
Query: 375 VLFPMMRSKGGISRGLVGRPTMIILPLGLSRPMYGAKVRFADTVLMMPSMV 223
VLF R+KGG S G GRPTM ILPLGLS P GA FADTV MMPSMV
Sbjct: 174 VLFLATRAKGGTSTGFSGRPTMTILPLGLSSPKRGAMGMFADTVFMMPSMV 22
Score = 132 bits (283), Expect = 3e-030
Identities = 62/119 (52%), Positives = 73/119 (61%)
Frame = -3 / -2
Query: 562 ILEGXXXXXXXXXXXVSLGQALHGRVHVVRGHQFYVRRHAVLGRHVDHLLRVPHAADAAP 383
++EG V LG+ALH RVHV R V RH + R V HLL V HAAD P
Sbjct: 361 LVEGVANVDDESIPDVRLGKALHSRVHVFRADHLDVCRHTMFRREVQHLLGVAHAADETP 182
Query: 382 GQGLVPNDEIKGRDLERLGGQADHDHLAVGLEQAHVRGQGEVRRHRVDDAVHGARSRLH 206
QGLV ++ KGR+L+ L +ADHDHLAVGLE RG G+V RHRV DA+HGA LH
Sbjct: 181 *QGLVLGNQSKGRNLDGLLWEADHDHLAVGLE*PEERGHGDVCRHRVYDAIHGAHRSLH 5
Score = 86.3 bits (182), Expect(2) = 4e-022
Identities = 37/64 (57%), Positives = 41/64 (64%)
Frame = +1 / +1
Query: 220 EHHGRHHQHGVGEPHLGPVHGPAQAQRQDDHGRPAHQASRDPAL*SHHWEQDPGRELHRR 399
EHHG HH+HGV + GP QAQRQD HGRP +A R +L S EQDP RE HRR
Sbjct: 19 EHHGWHHKHGVCKHPHGPSLRATQAQRQDGHGRPPREARRGSSLCSGCQEQDPVREFHRR 198
Query: 400 HEGH 411
HE H
Sbjct: 199 HERH 210
Score = 38.6 bits (78), Expect(2) = 4e-022
Identities = 15/23 (65%), Positives = 18/23 (78%)
Frame = +1 / +1
Query: 499 APGQG*RQVPLRHRHRQHPQEFR 567
AP QG RQV +RHRH QHP++ R
Sbjct: 298 APCQGGRQVSIRHRHWQHPRQGR 366
>gb|BJ549163.1|BJ549163 BJ549163 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags24k08 5', mRNA sequence
Length = 627
Score = 226 bits (487), Expect = 2e-058
Identities = 93/117 (79%), Positives = 107/117 (91%)
Frame = +2 / +1
Query: 206 MKAAASTMDGIINTVSANLTLAPYMGLLKPNGKMIMVGLPTKPLEIPPFDLIIGNKTLAG 385
MKAA STMDGIINTVSAN+ +AP GLLKPNGKM+MVGLP KP+E+PPF L+ NKTL+G
Sbjct: 34 MKAAMSTMDGIINTVSANIPMAPLFGLLKPNGKMVMVGLPEKPVEVPPFALVAKNKTLSG 213
Query: 386 SCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAMERLAKADVRYRFVIDIGNTL 556
S IGGM DTQEM+++AAKHGVTADIE+++A+YVNTAMERLAKADVRYRFVIDIGNTL
Sbjct: 214 SFIGGMSDTQEMLDLAAKHGVTADIEVISAEYVNTAMERLAKADVRYRFVIDIGNTL 384
Score = 147 bits (315), Expect = 1e-034
Identities = 70/111 (63%), Positives = 72/111 (64%)
Frame = -1 / -2
Query: 555 RVLPMSMTKRYLTSALARRXXXXXXXXXXXXXXXXXTPCLAATLIISCVSLMPPMQLPAR 376
RVLPMSMT RYLTSALARR TPC AA IS VSLMPPM+LP R
Sbjct: 383 RVLPMSMTNRYLTSALARRSIAVFTYSALITSMSAVTPCFAARSSISWVSLMPPMKLPDR 204
Query: 375 VLFPMMRSKGGISRGLVGRPTMIILPLGLSRPMYGAKVRFADTVLMMPSMV 223
VLF R+KGG S G GRPTM ILPLGLS P GA FADTV MMPSMV
Sbjct: 203 VLFLATRAKGGTSTGFSGRPTMTILPLGLSSPKRGAMGMFADTVFMMPSMV 51
Score = 134 bits (287), Expect = 9e-031
Identities = 63/119 (52%), Positives = 74/119 (62%)
Frame = -3 / -1
Query: 562 ILEGXXXXXXXXXXXVSLGQALHGRVHVVRGHQFYVRRHAVLGRHVDHLLRVPHAADAAP 383
++EG V LG+ALH RVHV R V RH +L R V HLL V HAAD P
Sbjct: 390 LVEGVANVDDESIPDVRLGKALHSRVHVFRADHLDVCRHTMLRREVQHLLGVAHAADETP 211
Query: 382 GQGLVPNDEIKGRDLERLGGQADHDHLAVGLEQAHVRGQGEVRRHRVDDAVHGARSRLH 206
QGLV ++ KGR+L+ L +ADHDHLAVGLE RG G+V RHRV DA+HGA LH
Sbjct: 210 *QGLVLGNQSKGRNLDGLLWEADHDHLAVGLE*PEERGHGDVCRHRVYDAIHGAHRSLH 34
Score = 86.3 bits (182), Expect(2) = 4e-022
Identities = 37/64 (57%), Positives = 41/64 (64%)
Frame = +1 / +3
Query: 220 EHHGRHHQHGVGEPHLGPVHGPAQAQRQDDHGRPAHQASRDPAL*SHHWEQDPGRELHRR 399
EHHG HH+HGV + GP QAQRQD HGRP +A R +L S EQDP RE HRR
Sbjct: 48 EHHGWHHKHGVCKHPHGPSLRATQAQRQDGHGRPPREARRGSSLCSGCQEQDPVREFHRR 227
Query: 400 HEGH 411
HE H
Sbjct: 228 HERH 239
Score = 38.6 bits (78), Expect(2) = 4e-022
Identities = 15/23 (65%), Positives = 18/23 (78%)
Frame = +1 / +3
Query: 499 APGQG*RQVPLRHRHRQHPQEFR 567
AP QG RQV +RHRH QHP++ R
Sbjct: 327 APCQGGRQVSIRHRHWQHPRQGR 395
>gb|BJ447263.1|BJ447263 BJ447263 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak14l05 5', mRNA sequence
Length = 575
Score = 222 bits (480), Expect = 2e-057
Identities = 89/151 (58%), Positives = 122/151 (80%)
Frame = +2 / +3
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
ISTSPGK++EAL+RLGAD F+VS+D E+M+AA TMDGI++TVSA ++P L+KP G
Sbjct: 24 ISTSPGKREEALERLGADEFLVSRDPEQMQAAVGTMDGILDTVSAWHPISPLFALMKPMG 203
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADY 481
+M+ VG PTKPLE+P + ++ G K +AG+C+GG+RD Q M+ AAKHG+TA++E++ DY
Sbjct: 204 QMVFVGGPTKPLELPAYAIVPGGKGIAGNCVGGIRDCQAMLEFAAKHGITAEVEVIKMDY 383
Query: 482 VNTAMERLAKADVRYRFVIDIGNTLKNSE*S 574
VNTA+ERLAK DVRYRFVIDI +L ++ *+
Sbjct: 384 VNTALERLAKNDVRYRFVIDIAGSLGSTS*A 476
Score = 101 bits (216), Expect = 6e-021
Identities = 53/119 (44%), Positives = 64/119 (53%)
Frame = -3 / -1
Query: 511 LGQALHGRVHVVRGHQFYVRRHAVLGRHVDHLLRVPHAADAAPGQGLVPNDEIKGRDLER 332
L + L RV VV + ++R AVL R H L VP AADA PG L + R L+R
Sbjct: 413 LREPLERRVDVVHLYHLHLRGDAVLCREFQHGLAVPDAADAVPGDALPARHDGVRRQLQR 234
Query: 331 LGGQADHDHLAVGLEQAHVRGQGEVRRHRVDDAVHGARSRLHFFGVLADDEGVGAKPVE 155
LG + HL L Q R RRHRV+DAVHGA RLH GV A E V A+P++
Sbjct: 233 LGRPSHEHHLPHRLHQREQRRDRVPRRHRVEDAVHGAHGRLHLLGVPAHQELVRAQPLQ 57
>gb|BJ454765.1|BJ454765 BJ454765 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak14l05 3', mRNA sequence
Length = 554
Score = 222 bits (480), Expect = 2e-057
Identities = 89/151 (58%), Positives = 122/151 (80%)
Frame = +2 / -3
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
ISTSPGK++EAL+RLGAD F+VS+D E+M+AA TMDGI++TVSA ++P L+KP G
Sbjct: 531 ISTSPGKREEALERLGADEFLVSRDPEQMQAAVGTMDGILDTVSAWHPISPLFALMKPMG 352
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADY 481
+M+ VG PTKPLE+P + ++ G K +AG+C+GG+RD Q M+ AAKHG+TA++E++ DY
Sbjct: 351 QMVFVGGPTKPLELPAYAIVPGGKGIAGNCVGGIRDCQAMLEFAAKHGITAEVEVIKMDY 172
Query: 482 VNTAMERLAKADVRYRFVIDIGNTLKNSE*S 574
VNTA+ERLAK DVRYRFVIDI +L ++ *+
Sbjct: 171 VNTALERLAKNDVRYRFVIDIAGSLGSTS*A 79
Score = 101 bits (216), Expect = 6e-021
Identities = 53/119 (44%), Positives = 64/119 (53%)
Frame = -3 / +1
Query: 511 LGQALHGRVHVVRGHQFYVRRHAVLGRHVDHLLRVPHAADAAPGQGLVPNDEIKGRDLER 332
L + L RV VV + ++R AVL R H L VP AADA PG L + R L+R
Sbjct: 142 LREPLERRVDVVHLYHLHLRGDAVLCREFQHGLAVPDAADAVPGDALPARHDGVRRQLQR 321
Query: 331 LGGQADHDHLAVGLEQAHVRGQGEVRRHRVDDAVHGARSRLHFFGVLADDEGVGAKPVE 155
LG + HL L Q R RRHRV+DAVHGA RLH GV A E V A+P++
Sbjct: 322 LGRPSHEHHLPHRLHQREQRRDRVPRRHRVEDAVHGAHGRLHLLGVPAHQELVRAQPLQ 498
>gb|BQ759606.1|BQ759606 EBpi03_SQ002_D17_R pistil, 4 DPA, no treatment, cv Optic, EBpi03
Hordeum vulgare subsp. vulgare cDNA clone
EBpi03_SQ002_D17 5', mRNA sequence
Length = 441
Score = 221 bits (477), Expect = 6e-057
Identities = 91/115 (79%), Positives = 105/115 (91%)
Frame = +2 / +1
Query: 212 AAASTMDGIINTVSANLTLAPYMGLLKPNGKMIMVGLPTKPLEIPPFDLIIGNKTLAGSC 391
AA STMDGIINTVSAN+ +AP GLLKPNGKM+MVGLP KP+E+PPF L+ NKTL+GS
Sbjct: 1 AAMSTMDGIINTVSANIPMAPLFGLLKPNGKMVMVGLPEKPVEVPPFALVAKNKTLSGSF 180
Query: 392 IGGMRDTQEMINVAAKHGVTADIELVAADYVNTAMERLAKADVRYRFVIDIGNTL 556
IGGM DTQEM+++AAKHGVTADIE+++A+YVNTAMERLAKADVRYRFVIDIGNTL
Sbjct: 181 IGGMSDTQEMLDLAAKHGVTADIEVISAEYVNTAMERLAKADVRYRFVIDIGNTL 345
Score = 147 bits (315), Expect = 1e-034
Identities = 70/111 (63%), Positives = 72/111 (64%)
Frame = -1 / -2
Query: 555 RVLPMSMTKRYLTSALARRXXXXXXXXXXXXXXXXXTPCLAATLIISCVSLMPPMQLPAR 376
RVLPMSMT RYLTSALARR TPC AA IS VSLMPPM+LP R
Sbjct: 344 RVLPMSMTNRYLTSALARRSIAVFTYSALITSMSAVTPCFAARSSISWVSLMPPMKLPDR 165
Query: 375 VLFPMMRSKGGISRGLVGRPTMIILPLGLSRPMYGAKVRFADTVLMMPSMV 223
VLF R+KGG S G GRPTM ILPLGLS P GA FADTV MMPSMV
Sbjct: 164 VLFLATRAKGGTSTGFSGRPTMTILPLGLSSPKRGAMGMFADTVFMMPSMV 12
Score = 127 bits (271), Expect = 2e-028
Identities = 57/96 (59%), Positives = 66/96 (68%)
Frame = -3 / -1
Query: 508 GQALHGRVHVVRGHQFYVRRHAVLGRHVDHLLRVPHAADAAPGQGLVPNDEIKGRDLERL 329
G+ALH RVHV R V RH +L R V HLL V HAAD P QGLV ++ KGR+L+ L
Sbjct: 297 GKALHSRVHVFRADHLDVCRHTMLRREVQHLLGVAHAADETP*QGLVLGNQSKGRNLDGL 118
Query: 328 GGQADHDHLAVGLEQAHVRGQGEVRRHRVDDAVHGA 221
+ADHDHLAVGLE RG G+V RHRV DA+HGA
Sbjct: 117 LWEADHDHLAVGLE*PEERGHGDVCRHRVYDAIHGA 10
Score = 86.3 bits (182), Expect(2) = 2e-021
Identities = 37/64 (57%), Positives = 41/64 (64%)
Frame = +1 / +3
Query: 220 EHHGRHHQHGVGEPHLGPVHGPAQAQRQDDHGRPAHQASRDPAL*SHHWEQDPGRELHRR 399
EHHG HH+HGV + GP QAQRQD HGRP +A R +L S EQDP RE HRR
Sbjct: 9 EHHGWHHKHGVCKHPHGPSLRATQAQRQDGHGRPPREARRGSSLCSGCQEQDPVREFHRR 188
Query: 400 HEGH 411
HE H
Sbjct: 189 HERH 200
Score = 36.3 bits (73), Expect(2) = 2e-021
Identities = 14/23 (60%), Positives = 17/23 (73%)
Frame = +1 / +3
Query: 499 APGQG*RQVPLRHRHRQHPQEFR 567
AP Q RQV +RHRH QHP++ R
Sbjct: 288 APCQSGRQVSIRHRHWQHPRQSR 356
>gb|BU977917.1|BU977917 HA12E09r HA Hordeum vulgare subsp. vulgare cDNA clone HA12E09
5-PRIME, mRNA sequence
Length = 573
Score = 201 bits (433), Expect(2) = 1e-054
Identities = 83/100 (83%), Positives = 93/100 (93%)
Frame = +2 / +3
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
IS+SPGKKQEAL+RLGADAFVVSKDA+EMKAA STMDGIINTVSAN+ +AP +GLLKPNG
Sbjct: 273 ISSSPGKKQEALERLGADAFVVSKDADEMKAAVSTMDGIINTVSANIPMAPLLGLLKPNG 452
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEM 421
KMIMVGLP +P+E+PPF L+ NKTLAGSCIGGMRDTQEM
Sbjct: 453 KMIMVGLPEQPIEVPPFALVARNKTLAGSCIGGMRDTQEM 572
Score = 181 bits (389), Expect = 8e-045
Identities = 82/140 (58%), Positives = 84/140 (60%)
Frame = -1 / -3
Query: 420 ISCVSLMPPMQLPARVLFPMMRSKGGISRGLVGRPTMIILPLGLSRPMYGAKVRFADTVL 241
IS VSLMPPMQLPARVLF R+KGG S G GRPTMIILPLGLS P GA FADTV
Sbjct: 571 ISWVSLMPPMQLPARVLFLATRAKGGTSIGCSGRPTMIILPLGLSSPRRGAMGMFADTVF 392
Query: 240 MMPSMVXXXXXXXXXXXXXXXXXXXSRSSASCFFPGDVLMXXXXXXXXXXXXXXTWPRPP 61
MMPSMV S S ASCFFPGD L+ TWP PP
Sbjct: 391 MMPSMVLTAAFISSASLLTTNASAPSLSRASCFFPGDELITVTFIPKALPNLTATWPSPP 212
Query: 60 SPITPTCFPGTFRPWYFMGL 1
SP TP C PGTF PWYFMGL
Sbjct: 211 SPSTPRCNPGTFSPWYFMGL 152
Score = 148 bits (318), Expect(2) = 7e-045
Identities = 63/89 (70%), Positives = 70/89 (78%)
Frame = -3 / -2
Query: 421 HLLRVPHAADAAPGQGLVPNDEIKGRDLERLGGQADHDHLAVGLEQAHVRGQGEVRRHRV 242
HLL VPHAADAAPGQGLVP ++ KGRDL+RL +ADHDHLAVGLE RG G+V RHRV
Sbjct: 572 HLLGVPHAADAAPGQGLVPGNQSKGRDLDRLLWEADHDHLAVGLE*PEERGHGDVCRHRV 393
Query: 241 DDAVHGARSRLHFFGVLADDEGVGAKPVE 155
DA+HGA LH GVLADDE V AKP+E
Sbjct: 392 YDAIHGAHRSLHLVGVLADDERVSAKPLE 306
Score = 101 bits (215), Expect(2) = 9e-024
Identities = 42/64 (65%), Positives = 45/64 (70%)
Frame = +1 / +2
Query: 220 EHHGRHHQHGVGEPHLGPVHGPAQAQRQDDHGRPAHQASRDPAL*SHHWEQDPGRELHRR 399
EHHG HH+HGV + GP QAQRQDDHGRP A R P+L S EQDPGRELHRR
Sbjct: 371 EHHGWHHKHGVCKHPHGPSPRATQAQRQDDHGRPPRAAYRGPSLCSGCQEQDPGRELHRR 550
Query: 400 HEGH 411
HEGH
Sbjct: 551 HEGH 562
Score = 52.8 bits (109), Expect(2) = 7e-045
Identities = 22/35 (62%), Positives = 26/35 (74%)
Frame = -3 / -2
Query: 106 PERLGELDGDVAQASQPHHADVLPRHVQAVVLHGA 2
P+ ELDGDVAQ++Q HA V P +VQ VVLHGA
Sbjct: 257 PKGFAELDGDVAQSAQSQHAKVQPGNVQPVVLHGA 153
Score = 32.7 bits (65), Expect(2) = 1e-054
Identities = 14/23 (60%), Positives = 14/23 (60%)
Frame = +3 / +1
Query: 3 AP*STTA*TCRGSTSA*WGWEAW 71
AP*STT *T G T A W W W
Sbjct: 154 AP*STTG*TFPGCTLACWDWADW 222
Score = 31.8 bits (63), Expect(2) = 9e-024
Identities = 11/11 (100%), Positives = 11/11 (100%)
Frame = +2 / +3
Query: 2 SPMKYHGLNVP 34
SPMKYHGLNVP
Sbjct: 153 SPMKYHGLNVP 185
>gb|AV916085.1|AV916085 AV916085 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags1g09 5', mRNA sequence
Length = 616
Score = 211 bits (456), Expect = 5e-054
Identities = 87/144 (60%), Positives = 116/144 (80%)
Frame = +2 / +2
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
IS+SP K++EAL+RLGADAF+VS+DAE+MKAAA TMDGII+TVSA L P + L+KP G
Sbjct: 71 ISSSPRKREEALERLGADAFLVSQDAEQMKAAAGTMDGIIDTVSAGHPLVPLLELMKPMG 250
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADY 481
+M++VG P++PL +P + +I G K LAG+ +G +RD Q M++ A KHG+TAD+E+V DY
Sbjct: 251 QMVVVGAPSEPLALPAYAIIGGGKRLAGNGVGSVRDCQAMLDFAGKHGITADVEVVKMDY 430
Query: 482 VNTAMERLAKADVRYRFVIDIGNT 553
VNTA+ERL K DVRYRFVID+ +
Sbjct: 431 VNTAVERLEKNDVRYRFVIDVAGS 502
Score = 108 bits (231), Expect(2) = 3e-025
Identities = 57/117 (48%), Positives = 64/117 (54%)
Frame = -3 / -1
Query: 505 QALHGRVHVVRGHQFYVRRHAVLGRHVDHLLRVPHAADAAPGQGLVPNDEIKGRDLERLG 326
Q L RV VV VR AVL R V H L V HAA A PGQ L +D+ G + L
Sbjct: 454 QPLDRRVDVVHLDDLDVRGDAVLPREVQHRLAVAHAAHAVPGQALAASDDGVGWQRQGLA 275
Query: 325 GQADHDHLAVGLEQAHVRGQGEVRRHRVDDAVHGARSRLHFFGVLADDEGVGAKPVE 155
+ADH HL L Q + RRHRVDDAVH R LH GVLAD E VGA+ +E
Sbjct: 274 RRADHHHLPHRLHQLQQWHERVPRRHRVDDAVHRPRRSLHLLGVLADQERVGAEALE 104
Score = 26.7 bits (52), Expect(2) = 3e-025
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = -3 / -1
Query: 106 PERLGELDGDVAQASQ 59
PERL ELDG VA+ +
Sbjct: 55 PERLAELDGHVAETPE 8
>gb|BJ548462.1|BJ548462 BJ548462 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal25k20 3', mRNA sequence
Length = 600
Score = 138 bits (296), Expect(2) = 5e-054
Identities = 57/69 (82%), Positives = 64/69 (92%)
Frame = +2 / -1
Query: 350 FDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAMERLAKADVRYR 529
F GNKTLAGSCIGGMRDTQEM+++AAKHGVTADIE+++A+YVNTAMERLAKADVRYR
Sbjct: 366 FSECTGNKTLAGSCIGGMRDTQEMLDLAAKHGVTADIEVISAEYVNTAMERLAKADVRYR 187
Query: 530 FVIDIGNTL 556
FVIDI NTL
Sbjct: 186 FVIDIANTL 160
Score = 93.6 bits (198), Expect(2) = 5e-054
Identities = 36/47 (76%), Positives = 43/47 (91%)
Frame = +2 / -2
Query: 221 STMDGIINTVSANLTLAPYMGLLKPNGKMIMVGLPTKPLEIPPFDLI 361
STMDGIINTVSAN+ +AP +GLLKPNGKMIMVGLP +P+E+PPF L+
Sbjct: 596 STMDGIINTVSANIPMAPLLGLLKPNGKMIMVGLPEQPIEVPPFALV 456
Score = 84.5 bits (178), Expect(2) = 4e-032
Identities = 40/65 (61%), Positives = 41/65 (63%)
Frame = -1 / +2
Query: 555 RVLPMSMTKRYLTSALARRXXXXXXXXXXXXXXXXXTPCLAATLIISCVSLMPPMQLPAR 376
RVL MSMT RYLTSALARR TPC AA IS VSLMPPMQLPAR
Sbjct: 161 RVLAMSMTNRYLTSALARRSIAVFTYSALITSMSAVTPCFAARSSISWVSLMPPMQLPAR 340
Query: 375 VLFPM 361
VLFP+
Sbjct: 341 VLFPV 355
Score = 83.5 bits (176), Expect(2) = 1e-031
Identities = 38/66 (57%), Positives = 44/66 (66%)
Frame = -3 / +1
Query: 562 ILEGXXXXXXXXXXXVSLGQALHGRVHVVRGHQFYVRRHAVLGRHVDHLLRVPHAADAAP 383
++EG +SLG+ALH VHV+R + V RHAVL R V HLL VPHAADAAP
Sbjct: 154 LVEGVGDVDDESIPDISLGKALHRCVHVLRAYHLDVCRHAVLRREVQHLLGVPHAADAAP 333
Query: 382 GQGLVP 365
GQGLVP
Sbjct: 334 GQGLVP 351
Score = 74.4 bits (156), Expect(2) = 4e-032
Identities = 31/47 (65%), Positives = 37/47 (78%)
Frame = -3 / +3
Query: 361 DEIKGRDLERLGGQADHDHLAVGLEQAHVRGQGEVRRHRVDDAVHGA 221
++ KGRDL+RL +ADHDHLAVGLE RG G+V RHRV DA+HGA
Sbjct: 456 NQSKGRDLDRLLWEADHDHLAVGLE*PEERGHGDVCRHRVYDAIHGA 596
Score = 73.5 bits (154), Expect(2) = 1e-031
Identities = 33/45 (73%), Positives = 34/45 (75%)
Frame = -1 / +1
Query: 357 RSKGGISRGLVGRPTMIILPLGLSRPMYGAKVRFADTVLMMPSMV 223
R+KGG S G GRPTMIILPLGLS P GA FADTV MMPSMV
Sbjct: 460 RAKGGTSIGCSGRPTMIILPLGLSSPRRGAMGMFADTVFMMPSMV 594
Score = 64.3 bits (134), Expect(3) = 2e-024
Identities = 26/44 (59%), Positives = 29/44 (65%)
Frame = +1 / -1
Query: 220 EHHGRHHQHGVGEPHLGPVHGPAQAQRQDDHGRPAHQASRDPAL 351
EHHG HH+HGV + GP QAQRQDDHGRP A R P+L
Sbjct: 597 EHHGWHHKHGVCKHPHGPSPRATQAQRQDDHGRPPRAAYRGPSL 466
Score = 45.5 bits (93), Expect(3) = 2e-024
Identities = 16/17 (94%), Positives = 16/17 (94%)
Frame = +1 / -3
Query: 361 HWEQDPGRELHRRHEGH 411
H EQDPGRELHRRHEGH
Sbjct: 355 HREQDPGRELHRRHEGH 305
Score = 40.9 bits (83), Expect(3) = 2e-024
Identities = 16/23 (69%), Positives = 19/23 (82%)
Frame = +1 / -3
Query: 499 APGQG*RQVPLRHRHRQHPQEFR 567
AP QG* QV +RHRHRQHP++ R
Sbjct: 217 APCQG*CQVSIRHRHRQHPRQGR 149
>gb|BF257150.3|BF257150 HVSMEf0012A06f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0012A06f, mRNA sequence
Length = 852
Score = 206 bits (445), Expect = 2e-052
Identities = 86/141 (60%), Positives = 114/141 (80%)
Frame = +2 / +2
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
IS+SP K++EAL+RLGADAF+VS+DAE+MKAAA TMDGII+TVSA L P + L+KP G
Sbjct: 56 ISSSPRKREEALERLGADAFLVSQDAEQMKAAAGTMDGIIDTVSAGHPLVPLLELMKPMG 235
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADY 481
+M++VG P++PL +P + +I G K LAG+ +G +RD Q M++ A KHG+TAD+E+V DY
Sbjct: 236 QMVVVGAPSEPLALPAYAIIGGGKRLAGNGVGSVRDCQAMLDFAGKHGITADVEVVKMDY 415
Query: 482 VNTAMERLAKADVRYRFVIDI 544
VNTA+ERL K DVR RFVID+
Sbjct: 416 VNTAVERLEKNDVRDRFVIDV 478
Score = 112 bits (240), Expect(2) = 1e-025
Identities = 58/117 (49%), Positives = 65/117 (55%)
Frame = -3 / -3
Query: 505 QALHGRVHVVRGHQFYVRRHAVLGRHVDHLLRVPHAADAAPGQGLVPNDEIKGRDLERLG 326
Q L RVHVV VR AVL R V H L V HAA A PGQ L +D+ G + L
Sbjct: 439 QPLDRRVHVVHLDDLDVRGDAVLPREVQHRLAVAHAAHAVPGQALAASDDGVGWQRQGLA 260
Query: 325 GQADHDHLAVGLEQAHVRGQGEVRRHRVDDAVHGARSRLHFFGVLADDEGVGAKPVE 155
+ADH HL L Q + RRHRVDDAVH R LH GVLAD E VGA+ +E
Sbjct: 259 RRADHHHLPHRLHQLQQWHERVPRRHRVDDAVHRPRRSLHLLGVLADQERVGAEALE 89
Score = 24.0 bits (46), Expect(2) = 1e-025
Identities = 9/13 (69%), Positives = 10/13 (76%)
Frame = -3 / -3
Query: 106 PERLGELDGDVAQ 68
PERL ELDG V +
Sbjct: 40 PERLAELDGHVPE 2
>gb|BU977918.1|BU977918 HA12E09u HA Hordeum vulgare subsp. vulgare cDNA clone HA12E09
3-PRIME, mRNA sequence
Length = 497
Score = 198 bits (427), Expect = 5e-050
Identities = 79/100 (79%), Positives = 93/100 (93%)
Frame = +2 / -3
Query: 257 NLTLAPYMGLLKPNGKMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAA 436
N+ +AP +GLLKPNGKMIMVGLP +P+E+PPF L+ NKTLAGSCIGGMRDTQEM+++AA
Sbjct: 495 NIPMAPLLGLLKPNGKMIMVGLPEQPIEVPPFALVARNKTLAGSCIGGMRDTQEMLDLAA 316
Query: 437 KHGVTADIELVAADYVNTAMERLAKADVRYRFVIDIGNTL 556
KHGVTADIE+++A+YVNTAMERLAKADVRYRFVIDI NTL
Sbjct: 315 KHGVTADIEVISAEYVNTAMERLAKADVRYRFVIDIANTL 196
Score = 133 bits (285), Expect = 2e-030
Identities = 61/102 (59%), Positives = 71/102 (69%)
Frame = -3 / +1
Query: 562 ILEGXXXXXXXXXXXVSLGQALHGRVHVVRGHQFYVRRHAVLGRHVDHLLRVPHAADAAP 383
++EG VSLG+ALH VHV+R + V RHAVL R V HLL VPHAADAAP
Sbjct: 190 LVEGVGDVDDESIPDVSLGKALHRCVHVLRAYHLDVCRHAVLRREVQHLLGVPHAADAAP 369
Query: 382 GQGLVPNDEIKGRDLERLGGQADHDHLAVGLEQAHVRGQGEV 257
GQGLVP ++ KGRDL+RL +ADHDHLAVGLE RG G+V
Sbjct: 370 GQGLVPGNQSKGRDLDRLLWEADHDHLAVGLE*PEERGHGDV 495
Score = 126 bits (270), Expect = 2e-028
Identities = 62/100 (62%), Positives = 63/100 (63%)
Frame = -1 / +2
Query: 555 RVLPMSMTKRYLTSALARRXXXXXXXXXXXXXXXXXTPCLAATLIISCVSLMPPMQLPAR 376
RVL MSMT RYLTSALARR TPC AA IS VSLMPPMQLPAR
Sbjct: 197 RVLAMSMTNRYLTSALARRSIAVFTYSALITSMSAVTPCFAARSSISWVSLMPPMQLPAR 376
Query: 375 VLFPMMRSKGGISRGLVGRPTMIILPLGLSRPMYGAKVRF 256
VLF R+KGG S G GRPTMIILPLGLS P GA F
Sbjct: 377 VLFLATRAKGGTSIGCSGRPTMIILPLGLSSPRRGAMGMF 496
Score = 78.5 bits (165), Expect(2) = 2e-021
Identities = 33/48 (68%), Positives = 34/48 (70%)
Frame = +1 / -2
Query: 268 GPVHGPAQAQRQDDHGRPAHQASRDPAL*SHHWEQDPGRELHRRHEGH 411
GP QAQRQDDHGRP A R P+L S EQDPGRELHRRHEGH
Sbjct: 484 GPSPRATQAQRQDDHGRPPRAAYRGPSLCSGCQEQDPGRELHRRHEGH 341
Score = 44.6 bits (91), Expect(2) = 2e-021
Identities = 17/23 (73%), Positives = 20/23 (86%)
Frame = +1 / -2
Query: 499 APGQG*RQVPLRHRHRQHPQEFR 567
AP QG*RQV +RHRHRQHP++ R
Sbjct: 253 APCQG*RQVSIRHRHRQHPRQGR 185
>gb|CB879610.1|CB879610 HP02P10w HP Hordeum vulgare subsp. vulgare cDNA clone HP02P10
3-PRIME, mRNA sequence
Length = 667
Score = 182 bits (393), Expect = 2e-045
Identities = 74/141 (52%), Positives = 108/141 (76%)
Frame = +2 / -1
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
ISTSP K++EA + L AD FV+S D ++M+A A ++D +I+TVSA +L P + LLK NG
Sbjct: 628 ISTSPAKEREARESLKADDFVLSTDDKQMQAMARSLDYVIDTVSAQHSLGPILELLKVNG 449
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADY 481
K+++V P KP+E+P F LI G +T++GS GGM++TQEM+++ +HG+TADIELV+
Sbjct: 448 KLVLVAAPDKPVELPSFPLIFGKRTVSGSMTGGMKETQEMMDLCGEHGITADIELVSTGG 269
Query: 482 VNTAMERLAKADVRYRFVIDI 544
+N A+ RLA+ DVRYRFV+D+
Sbjct: 268 INDALARLARNDVRYRFVVDV 206
>gb|CA001190.1|CA001190 HS05A02u HS Hordeum vulgare subsp. vulgare cDNA clone HS05A02
3-PRIME, mRNA sequence
Length = 513
Score = 176 bits (378), Expect = 3e-043
Identities = 72/123 (58%), Positives = 96/123 (78%)
Frame = +2 / -2
Query: 185 VSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNGKMIMVGLPTKPLEIPPFDLII 364
VS+DAE+MKAAA TMDGII+TVSA L P + L+KP G+M++VG P++PL +P + +I
Sbjct: 512 VSQDAEQMKAAAGTMDGIIDTVSAGHPLVPLLELMKPMGQMVVVGAPSEPLALPAYAIIG 333
Query: 365 GNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAMERLAKADVRYRFVIDI 544
G K LAG+ +G +RD Q M++ A KHG+TAD+E+V DYVNTA+ERL K DVRYRFVID+
Sbjct: 332 GGKRLAGNGVGSVRDCQAMLDFAGKHGITADVEVVKMDYVNTAVERLEKNDVRYRFVIDV 153
Query: 545 GNT 553
+
Sbjct: 152 AGS 144
>gb|CB880789.1|CB880789 HM07N11w HM Hordeum vulgare subsp. vulgare cDNA clone HM07N11
3-PRIME, mRNA sequence
Length = 479
Score = 164 bits (352), Expect = 1e-039
Identities = 68/86 (79%), Positives = 77/86 (89%)
Frame = +2 / -3
Query: 308 IMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADYVN 487
IMVGLP P+EI PF L+ NKTLAGSCIGGMRDTQEM+++AAKH VTADIE++ A+YVN
Sbjct: 471 IMVGLPENPMEISPFALVATNKTLAGSCIGGMRDTQEMLDLAAKHDVTADIEVIDAEYVN 292
Query: 488 TAMERLAKADVRYRFVIDIGNTLKNS 565
TAMERLAKADVRYRFVIDIGNTL N+
Sbjct: 291 TAMERLAKADVRYRFVIDIGNTLDNA 214
Score = 103 bits (220), Expect = 2e-021
Identities = 50/85 (58%), Positives = 53/85 (62%)
Frame = -1 / +2
Query: 555 RVLPMSMTKRYLTSALARRXXXXXXXXXXXXXXXXXTPCLAATLIISCVSLMPPMQLPAR 376
RVLPMSMT RYLTSALA R T C AA IS VSLMPPMQLPAR
Sbjct: 224 RVLPMSMTNRYLTSALASRSMAVFTYSASITSMSAVTSCFAARSSISWVSLMPPMQLPAR 403
Query: 375 VLFPMMRSKGGISRGLVGRPTMIIL 301
VLF R+KG IS G GRPTMI++
Sbjct: 404 VLFVATRAKGEISIGFSGRPTMILV 478
>gb|BU994127.1|BU994127 HM05N11r HM Hordeum vulgare subsp. vulgare cDNA clone HM05N11
5-PRIME, mRNA sequence
Length = 480
Score = 162 bits (348), Expect = 4e-039
Identities = 67/85 (78%), Positives = 76/85 (89%)
Frame = +2 / +1
Query: 311 MVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNT 490
MVGLP P+EI PF L+ NKTLAGSCIGGMRDTQEM+++AAKH VTADIE++ A+YVNT
Sbjct: 13 MVGLPENPMEISPFALVATNKTLAGSCIGGMRDTQEMLDLAAKHDVTADIEVIDAEYVNT 192
Query: 491 AMERLAKADVRYRFVIDIGNTLKNS 565
AMERLAKADVRYRFVIDIGNTL N+
Sbjct: 193 AMERLAKADVRYRFVIDIGNTLDNA 267
>gb|BG310264.2|BG310264 HVSMEc0016L11f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0016L11f, mRNA sequence
Length = 758
Score = 160 bits (344), Expect = 1e-038
Identities = 65/122 (53%), Positives = 91/122 (74%)
Frame = +2 / -1
Query: 188 SKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNGKMIMVGLPTKPLEIPPFDLIIG 367
++ ++KA+A TM GIINTVSA L P + L+KP G+M++VG P++PL +P + +I G
Sbjct: 725 ARTPNQIKASAGTMYGIINTVSAGHPLVPLLELIKPMGQMVVVGAPSEPLALPAYAIIGG 546
Query: 368 NKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAMERLAKADVRYRFVIDIG 547
K LAG+ +G +RD Q M++ A KHG+TAD+E+V DYVNTA+ERL K DVRYRFVID+
Sbjct: 545 GKRLAGNGVGSVRDCQAMLDFAGKHGITADVEVVKMDYVNTAVERLEKNDVRYRFVIDVA 366
Query: 548 NT 553
+
Sbjct: 365 GS 360
>gb|BQ660971.1|BQ660971 HM01F04w HM Hordeum vulgare subsp. vulgare cDNA clone HM01F04
3-PRIME, mRNA sequence
Length = 600
Score = 157 bits (338), Expect = 9e-038
Identities = 66/144 (45%), Positives = 100/144 (69%)
Frame = +2 / -2
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
IS+S K+ EA+D LGAD ++VS DA++M AAA ++D II+TV L PY+ LLK +G
Sbjct: 551 ISSSNKKRAEAVDDLGADEYLVSSDADQMAAAADSLDYIIDTVPVKHPLEPYLALLKMDG 372
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADY 481
K++++G+ +PL +++G KT+ GS IG M +T+E++ G+T+ IE+V DY
Sbjct: 371 KLVLMGVIAEPLSFVSPMVMLGRKTITGSFIGSMDETEEVLKFCVDKGLTSQIEVVKMDY 192
Query: 482 VNTAMERLAKADVRYRFVIDIGNT 553
VN A+ERL + DVRYRFV+D+ +
Sbjct: 191 VNQALERLERNDVRYRFVVDVAGS 120
>gb|CB881341.1|CB881341 HM09I05w HM Hordeum vulgare subsp. vulgare cDNA clone HM09I05
3-PRIME, mRNA sequence
Length = 621
Score = 157 bits (338), Expect = 9e-038
Identities = 66/144 (45%), Positives = 100/144 (69%)
Frame = +2 / -2
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
IS+S K+ EA+D LGAD ++VS DA++M AAA ++D II+TV L PY+ LLK +G
Sbjct: 572 ISSSNKKRAEAVDDLGADEYLVSSDADQMAAAADSLDYIIDTVPVKHPLEPYLALLKMDG 393
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADY 481
K++++G+ +PL +++G KT+ GS IG M +T+E++ G+T+ IE+V DY
Sbjct: 392 KLVLMGVIAEPLSFVSPMVMLGRKTITGSFIGSMDETEEVLKFCVDKGLTSQIEVVKMDY 213
Query: 482 VNTAMERLAKADVRYRFVIDIGNT 553
VN A+ERL + DVRYRFV+D+ +
Sbjct: 212 VNQALERLERNDVRYRFVVDVAGS 141
>gb|AV917273.1|AV917273 AV917273 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags18d14 5', mRNA sequence
Length = 546
Score = 155 bits (333), Expect = 4e-037
Identities = 60/115 (52%), Positives = 90/115 (78%)
Frame = +2 / +3
Query: 200 EEMKAAASTMDGIINTVSANLTLAPYMGLLKPNGKMIMVGLPTKPLEIPPFDLIIGNKTL 379
++M+A A ++D +I+TVSA +L P + LLK NGK+++V P KP+E+P F LI G +T+
Sbjct: 3 KQMQAMARSLDYVIDTVSAQHSLGPILELLKVNGKLVLVAAPDKPVELPSFPLIFGKRTV 182
Query: 380 AGSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAMERLAKADVRYRFVIDI 544
+GS GGM++TQEM+++ +HG+TADIELV+ D +N A+ RLA+ DVRYRFV+D+
Sbjct: 183 SGSMTGGMKETQEMMDLCGEHGITADIELVSTDGINDALARLARNDVRYRFVVDV 347
>gb|CB858813.1|CB858813 HI08G13w HI Hordeum vulgare subsp. vulgare cDNA clone HI08G13
3-PRIME, mRNA sequence
Length = 579
Score = 153 bits (329), Expect = 2e-036
Identities = 63/138 (45%), Positives = 96/138 (69%)
Frame = +2 / -3
Query: 140 KKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNGKMIMVG 319
K+ EA+D LGAD ++VS DA++M AAA ++D II+TV L PY+ LLK +GK++++G
Sbjct: 577 KRAEAVDDLGADEYLVSSDADQMAAAADSLDYIIDTVPVKHPLEPYLALLKMDGKLVLMG 398
Query: 320 LPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAME 499
+ +PL +++G KT+ GS IG M +T+E++ G+T+ IE+V DYVN A+E
Sbjct: 397 VIAEPLSFVSPMVMLGRKTITGSFIGSMDETEEVLKFCVDKGLTSQIEVVKMDYVNQALE 218
Query: 500 RLAKADVRYRFVIDIGNT 553
RL + DVRYRFV+D+ +
Sbjct: 217 RLERNDVRYRFVVDVAGS 164
>gb|BJ456704.1|BJ456704 BJ456704 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak23a07 3', mRNA sequence
Length = 674
Score = 96.4 bits (204), Expect(2) = 1e-035
Identities = 40/75 (53%), Positives = 53/75 (70%)
Frame = +2 / -1
Query: 335 LEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAMERLAKA 514
+ + P L +G +TL+GS GG +DTQEMIN A + + DIEL+ DY+N A+ERL
Sbjct: 335 IRVHPATLNLGARTLSGSVTGGTKDTQEMINFCAANKIYPDIELIKIDYINQALERLVNR 156
Query: 515 DVRYRFVIDIGNTLK 559
DVRYRFVIDI ++LK
Sbjct: 155 DVRYRFVIDIESSLK 111
Score = 74.4 bits (156), Expect(2) = 1e-035
Identities = 31/68 (45%), Positives = 46/68 (67%)
Frame = +2 / -1
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
+STS K+ EA++ LGAD FVVS D ++M++ S++D I++T S + PY+ LLK G
Sbjct: 545 LSTSESKRDEAINLLGADNFVVSSDKKQMESLKSSLDFIVDTASGDHPFDPYLALLKVGG 366
Query: 302 KMIMVGLP 325
M +VG P
Sbjct: 365 LMALVGFP 342
>gb|BU999880.1|BU999880 HS17B20u HS Hordeum vulgare subsp. vulgare cDNA clone HS17B20
3-PRIME, mRNA sequence
Length = 545
Score = 96.4 bits (204), Expect(2) = 1e-035
Identities = 40/75 (53%), Positives = 53/75 (70%)
Frame = +2 / -1
Query: 335 LEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAMERLAKA 514
+ + P L +G +TL+GS GG +DTQEMIN A + + DIEL+ DY+N A+ERL
Sbjct: 332 IRVHPATLNLGARTLSGSVTGGTKDTQEMINFCAANKIYPDIELIKIDYINQALERLVNR 153
Query: 515 DVRYRFVIDIGNTLK 559
DVRYRFVIDI ++LK
Sbjct: 152 DVRYRFVIDIESSLK 108
Score = 74.4 bits (156), Expect(2) = 1e-035
Identities = 31/68 (45%), Positives = 46/68 (67%)
Frame = +2 / -1
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
+STS K+ EA++ LGAD FVVS D ++M++ S++D I++T S + PY+ LLK G
Sbjct: 542 LSTSESKRDEAINLLGADNFVVSSDKKQMESLKSSLDFIVDTASGDHPFDPYLALLKVGG 363
Query: 302 KMIMVGLP 325
M +VG P
Sbjct: 362 LMALVGFP 339
>gb|BI957127.1|BI957127 HVSMEn0007J20f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0007J20f, mRNA sequence
Length = 876
Score = 149 bits (320), Expect = 3e-035
Identities = 65/144 (45%), Positives = 98/144 (68%)
Frame = +2 / +2
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
IS+S K+ EA+D LGAD ++VS DA++M AAA ++D II+TV L PY+ LLK +G
Sbjct: 38 ISSSNRKRAEAVDDLGADEYLVSSDADQMAAAADSLDYIIDTVPVKHPLEPYLALLKMDG 217
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADY 481
K++++G+ +PL +++G KT+ GS IG M +T+E++ G+T+ I +V DY
Sbjct: 218 KLVLMGVIAEPLSFVSPMVMLGRKTITGSFIGSMDETEEVLKFCVDKGLTSQIYVVKMDY 397
Query: 482 VNTAMERLAKADVRYRFVIDIGNT 553
VN A+ERL + DVRYRF + +G T
Sbjct: 398 VNHALERLERNDVRYRFGVYVGWT 469
>gb|AV931399.1|AV931399 AV931399 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd19k10 3', mRNA sequence
Length = 683
Score = 94.5 bits (200), Expect(2) = 4e-035
Identities = 39/75 (52%), Positives = 53/75 (70%)
Frame = +2 / -2
Query: 335 LEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAMERLAKA 514
+ + P L +G +TL+GS GG +DTQEMIN A + + DIEL+ DY+N A+ERL
Sbjct: 301 IRVHPATLNLGARTLSGSVTGGTKDTQEMINFCAVNKIYPDIELIKIDYINEALERLVNR 122
Query: 515 DVRYRFVIDIGNTLK 559
DV+YRFVIDI ++LK
Sbjct: 121 DVKYRFVIDIESSLK 77
Score = 74.4 bits (156), Expect(2) = 4e-035
Identities = 31/68 (45%), Positives = 46/68 (67%)
Frame = +2 / -2
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
+STS K+ EA++ LGAD FVVS D ++M++ S++D I++T S + PY+ LLK G
Sbjct: 511 LSTSESKRDEAINLLGADNFVVSSDKKQMESLKSSLDFIVDTASGDHPFDPYLALLKVGG 332
Query: 302 KMIMVGLP 325
M +VG P
Sbjct: 331 LMALVGFP 308
>gb|AV922443.1|AV922443 AV922443 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags18d14 3', mRNA sequence
Length = 520
Score = 148 bits (317), Expect = 7e-035
Identities = 57/106 (53%), Positives = 83/106 (78%)
Frame = +2 / -2
Query: 227 MDGIINTVSANLTLAPYMGLLKPNGKMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMR 406
+D +I+TVSA +L P + LLK NGK+++V P KP+E+P F LI G +T++GS GGM+
Sbjct: 519 LDYVIDTVSAQHSLGPILELLKVNGKLVLVAAPDKPVELPSFPLIFGKRTVSGSMTGGMK 340
Query: 407 DTQEMINVAAKHGVTADIELVAADYVNTAMERLAKADVRYRFVIDI 544
+TQEM+++ +HG+TADIELV+ D +N A+ RLA+ DVRYRFV+D+
Sbjct: 339 ETQEMMDLCGEHGITADIELVSTDGINDALARLARNDVRYRFVVDV 202
>gb|BF621349.2|BF621349 HVSMEa0006J13f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0006J13f, mRNA sequence
Length = 864
Score = 112 bits (240), Expect(2) = 6e-034
Identities = 51/117 (43%), Positives = 76/117 (64%)
Frame = +2 / +2
Query: 188 SKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNGKMIMVGLPTKPLEIPPFDLIIG 367
+K + + AA TMDGII TVSA + P + LLKP +++VG P+ PL++P + +I G
Sbjct: 134 AKTPSK*RLAAGTMDGIIYTVSAGHPIVPLLELLKPPRALVVVGAPSTPLQLPAYAIICG 313
Query: 368 NKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAMERLAKADVRYRFVI 538
K +AG+ +G + M++ AAKH +TAD+E+V D VN+A+ RL +RY FVI
Sbjct: 314 GKRVAGNLVGCVCSCHAMLDFAAKHCITADVEVVKMDSVNSAV*RLDYD*LRYPFVI 484
Score = 51.9 bits (107), Expect(2) = 6e-034
Identities = 21/31 (67%), Positives = 28/31 (90%)
Frame = +2 / +1
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKA 214
IS+SP K++EA+ RLGADAF+VS+D E+MKA
Sbjct: 67 ISSSPRKREEAVKRLGADAFLVSQDPEQMKA 159
>gb|BQ458310.1|BQ458310 HA05J23r HA Hordeum vulgare subsp. vulgare cDNA clone HA05J23
5-PRIME, mRNA sequence
Length = 427
Score = 132 bits (282), Expect(2) = 6e-034
Identities = 56/65 (86%), Positives = 62/65 (95%)
Frame = +2 / +2
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
IS+SPGKKQEAL+RLGADAFVVSKDA+EMKAA STMDGIINTVSAN+ +AP +GLLKPNG
Sbjct: 233 ISSSPGKKQEALERLGADAFVVSKDADEMKAAVSTMDGIINTVSANIPMAPLLGLLKPNG 412
Query: 302 KMIMV 316
KMIMV
Sbjct: 413 KMIMV 427
Score = 126 bits (269), Expect = 3e-028
Identities = 56/105 (53%), Positives = 57/105 (54%)
Frame = -1 / -2
Query: 315 TMIILPLGLSRPMYGAKVRFADTVLMMPSMVXXXXXXXXXXXXXXXXXXXSRSSASCFFP 136
TMIILPLGLS P GA FADTV MMPSMV S S ASCFFP
Sbjct: 426 TMIILPLGLSSPRRGAMGMFADTVFMMPSMVLTAAFISSASLLTTNASAPSLSRASCFFP 247
Query: 135 GDVLMXXXXXXXXXXXXXXTWPRPPSPITPTCFPGTFRPWYFMGL 1
GD L+ TWP PPSP TP C PGTF PWYFMGL
Sbjct: 246 GDELITVTFIPKALPNLTATWPSPPSPSTPRCNPGTFSPWYFMGL 112
Score = 88.1 bits (186), Expect(2) = 8e-027
Identities = 37/54 (68%), Positives = 40/54 (74%)
Frame = -3 / -1
Query: 316 DHDHLAVGLEQAHVRGQGEVRRHRVDDAVHGARSRLHFFGVLADDEGVGAKPVE 155
DHDHLAVGLE RG G+V RHRV DA+HGA LH GVLADDE V AKP+E
Sbjct: 427 DHDHLAVGLE*PEERGHGDVCRHRVYDAIHGAHRSLHLVGVLADDERVSAKPLE 266
Score = 52.8 bits (109), Expect(2) = 8e-027
Identities = 22/35 (62%), Positives = 26/35 (74%)
Frame = -3 / -1
Query: 106 PERLGELDGDVAQASQPHHADVLPRHVQAVVLHGA 2
P+ ELDGDVAQ++Q HA V P +VQ VVLHGA
Sbjct: 217 PKGFAELDGDVAQSAQSQHAKVQPGNVQPVVLHGA 113
Score = 32.7 bits (65), Expect(2) = 6e-034
Identities = 14/23 (60%), Positives = 14/23 (60%)
Frame = +3 / +3
Query: 3 AP*STTA*TCRGSTSA*WGWEAW 71
AP*STT *T G T A W W W
Sbjct: 114 AP*STTG*TFPGCTLACWDWADW 182
>gb|BF254987.2|BF254987 HVSMEf0005J24f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0005J24f, mRNA sequence
Length = 595
Score = 143 bits (307), Expect = 2e-033
Identities = 60/110 (54%), Positives = 86/110 (78%)
Frame = +2 / +2
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
IS+SP K++EAL+RLGADAF+VS+DAE+MKAAA TMDGII+TVSA L P + L+KP G
Sbjct: 254 ISSSPRKREEALERLGADAFLVSQDAEQMKAAAGTMDGIIDTVSAVHPLVPLLELMKPMG 433
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVT 451
+M++VG P++PL +P + +I K LAG+ + +R+ Q +++ A KHG+T
Sbjct: 434 QMVVVGAPSEPLALPAYPIIRCCKRLAGNGVASVRNCQAILDFAGKHGIT 583
Score = 93.6 bits (198), Expect(2) = 2e-026
Identities = 47/97 (48%), Positives = 55/97 (56%)
Frame = -3 / -1
Query: 445 AVLGRHVDHLLRVPHAADAAPGQGLVPNDEIKGRDLERLGGQADHDHLAVGLEQAHVRGQ 266
AVL R V + L V HA A PGQ L +D+ G + L +ADH HL L Q +
Sbjct: 577 AVLPREVQNGLAVAHAGHAVPGQALAASDDGVGWQRQGLAWRADHHHLPHRLHQLQQWHE 398
Query: 265 GEVRRHRVDDAVHGARSRLHFFGVLADDEGVGAKPVE 155
RRHRVDDAVH R LH GVLAD E VGA+ +E
Sbjct: 397 RVHRRHRVDDAVHRPRRSLHLLGVLADQERVGAEALE 287
Score = 46.0 bits (94), Expect(2) = 2e-026
Identities = 19/35 (54%), Positives = 25/35 (71%)
Frame = -3 / -1
Query: 106 PERLGELDGDVAQASQPHHADVLPRHVQAVVLHGA 2
PERL ELDG VA+ + ++VLPR V+AV+ H A
Sbjct: 238 PERLAELDGHVAETPEADDSEVLPRRVEAVLHHRA 134
>gb|BI957446.1|BI957446 HVSMEn0009J16f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0009J16f, mRNA sequence
Length = 646
Score = 140 bits (301), Expect(2) = 2e-033
Identities = 60/134 (44%), Positives = 92/134 (68%)
Frame = +2 / +2
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
IS+S K+ EA+D LGAD ++VS DA++M AAA ++D II+TV L PY+ LLK +G
Sbjct: 242 ISSSNKKRAEAVDDLGADEYLVSSDADQMAAAADSLDYIIDTVPVKHPLEPYLALLKMDG 421
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADY 481
K++++G+ +PL +++G KT+ GS IG M +T+E++ G+T+ IE+V DY
Sbjct: 422 KLVLMGVIAEPLSFVSPMVMLGRKTITGSFIGSMDETEEVLKFCVDKGLTSQIEVVKMDY 601
Query: 482 VNTAMERLAKADVR 523
VN A+E+L + DVR
Sbjct: 602 VNXALEKLERNDVR 643
Score = 22.1 bits (42), Expect(2) = 2e-033
Identities = 10/23 (43%), Positives = 12/23 (52%)
Frame = +3 / +3
Query: 3 AP*STTA*TCRGSTSA*WGWEAW 71
A *ST+ * S + W W AW
Sbjct: 123 AR*STSG**PPASAAGSWAWAAW 191
>emb|X92754.1|HVCADMRNA H.vulgare mRNA for cinnamyl alcohol dehydrogenase
Length = 458
Score = 140 bits (300), Expect = 2e-032
Identities = 60/136 (44%), Positives = 94/136 (69%)
Frame = +2 / +1
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
IS+S K+QEAL+ LGAD ++VS D+ M+ A ++D II+TV L PY+ LL+ +G
Sbjct: 49 ISSSDKKRQEALEHLGADDYLVSSDSTRMQEVADSLDYIIDTVPVFHPLEPYLSLLRLDG 228
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADY 481
K+I++G+ PL+ +++G KT+ GS +G +++T+EM+ + G+++ IE+V DY
Sbjct: 229 KLILMGVINTPLQFLTPVVMLGRKTITGSFVGSIKETEEMLEFCKEKGLSSMIEVVKMDY 408
Query: 482 VNTAMERLAKADVRYR 529
VN A+ERL K DVRYR
Sbjct: 409 VNKALERLEKNDVRYR 456
>gb|CB881629.1|CB881629 HM10G11w HM Hordeum vulgare subsp. vulgare cDNA clone HM10G11
3-PRIME, mRNA sequence
Length = 524
Score = 139 bits (299), Expect = 2e-032
Identities = 57/128 (44%), Positives = 88/128 (68%)
Frame = +2 / -1
Query: 170 ADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNGKMIMVGLPTKPLEIPP 349
AD ++VS DA++M AAA ++D II+TV L PY+ LLK +GK++++G+ +PL
Sbjct: 524 ADEYLVSSDADQMAAAADSLDYIIDTVPVKHPLEPYLALLKMDGKLVLMGVIAEPLSFVS 345
Query: 350 FDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAMERLAKADVRYR 529
+++G KT+ GS IG M +T+E++ G+T+ IE+V DYVN A+ERL + DVRYR
Sbjct: 344 PMVMLGRKTITGSFIGSMDETEEVLKFCVDKGLTSQIEVVKMDYVNQALERLERNDVRYR 165
Query: 530 FVIDIGNT 553
FV+D+ +
Sbjct: 164 FVVDVAGS 141
>gb|AL509755.1|AL509755 AL509755 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY02M11u 3',
mRNA sequence
Length = 695
Score = 96.4 bits (204), Expect(2) = 6e-031
Identities = 40/75 (53%), Positives = 53/75 (70%)
Frame = +2 / -1
Query: 335 LEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAMERLAKA 514
+ + P L +G +TL+GS GG +DTQEMIN A + + DIEL+ DY+N A+ERL
Sbjct: 365 IRVHPATLNLGARTLSGSVTGGTKDTQEMINFCAANKIYPDIELIKIDYINQALERLVNR 186
Query: 515 DVRYRFVIDIGNTLK 559
DVRYRFVIDI ++LK
Sbjct: 185 DVRYRFVIDIESSLK 141
Score = 58.3 bits (121), Expect(2) = 6e-031
Identities = 24/53 (45%), Positives = 35/53 (66%)
Frame = +2 / -1
Query: 167 GADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNGKMIMVGLP 325
GAD FVVS D ++M++ S++D I++T S + PY+ LLK G M +VG P
Sbjct: 530 GADNFVVSSDKKQMESLKSSLDFIVDTASGDHPFDPYLALLKVGGLMALVGFP 372
>gb|BQ661428.1|BQ661428 HM03H06u HM Hordeum vulgare subsp. vulgare cDNA clone HM03H06
3-PRIME, mRNA sequence
Length = 486
Score = 132 bits (282), Expect = 5e-030
Identities = 54/122 (44%), Positives = 83/122 (68%)
Frame = +2 / -2
Query: 188 SKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNGKMIMVGLPTKPLEIPPFDLIIG 367
S DA++M AAA ++D II+TV L PY+ LLK +GK++++G+ +PL +++G
Sbjct: 485 SSDADQMAAAADSLDYIIDTVPVKHPLEPYLALLKMDGKLVLMGVIAEPLSFVSPMVMLG 306
Query: 368 NKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAMERLAKADVRYRFVIDIG 547
KT+ GS IG M +T+E++ G+T+ IE+V DYVN A+ERL + DVRYRFV+D+
Sbjct: 305 RKTITGSFIGSMDETEEVLKFCVDKGLTSQIEVVKMDYVNQALERLERNDVRYRFVVDVA 126
Query: 548 NT 553
+
Sbjct: 125 GS 120
>gb|BI947283.1|BI947283 HVSMEl0005A06f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0005A06f, mRNA sequence
Length = 994
Score = 100 bits (213), Expect(2) = 6e-030
Identities = 38/78 (48%), Positives = 57/78 (73%)
Frame = +2 / +2
Query: 311 MVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNT 490
+VG P++PL +P + +I G K LAG+ +G +RD Q M++ A KHG+TAD+E+ DY+NT
Sbjct: 389 VVGAPSEPLALPAYAIIGGGKRLAGNGVGSVRDCQAMLDFAGKHGITADVEVFKKDYLNT 568
Query: 491 AMERLAKADVRYRFVIDI 544
A+ERL D+RYRF+ +
Sbjct: 569 AVERLENNDLRYRFLXQL 622
Score = 56.5 bits (117), Expect(3) = 1e-025
Identities = 27/51 (52%), Positives = 30/51 (58%)
Frame = -3 / -3
Query: 307 HLAVGLEQAHVRGQGEVRRHRVDDAVHGARSRLHFFGVLADDEGVGAKPVE 155
HL L Q + RRHRVDDAVH R LH GVLAD E VGA+ +E
Sbjct: 386 HLPHRLHQLQQWHERVPRRHRVDDAVHRPRRSLHLLGVLADQERVGAEALE 234
Score = 54.2 bits (112), Expect(3) = 1e-025
Identities = 30/71 (42%), Positives = 35/71 (49%)
Frame = -3 / -1
Query: 505 QALHGRVHVVRGHQFYVRRHAVLGRHVDHLLRVPHAADAAPGQGLVPNDEIKGRDLERLG 326
Q L V VV VR AVL R V H L V HAA A PGQ L +D+ G + L
Sbjct: 583 QPLDRGVEVVLFEDLDVRGDAVLPREVQHRLAVAHAAHAVPGQALAASDDGVGWQRQGLA 404
Query: 325 GQADHDHLAVG 293
+ADH +G
Sbjct: 403 RRADHTICPIG 371
Score = 51.0 bits (105), Expect(2) = 6e-030
Identities = 23/38 (60%), Positives = 25/38 (65%)
Frame = +3 / +1
Query: 171 PTPSSSARTPKK*RRLRAPWTASSTRCRRTSPWPRTWA 284
PT S SARTP K*R R WTASSTRCRR + W+
Sbjct: 250 PTRSWSARTPSK*RLRRGRWTASSTRCRRGTRSCHCWS 363
Score = 44.1 bits (90), Expect(3) = 1e-025
Identities = 18/33 (54%), Positives = 24/33 (72%)
Frame = -3 / -3
Query: 106 PERLGELDGDVAQASQPHHADVLPRHVQAVVLH 8
PERL ELDG VA+ + ++VLPR V+AV+ H
Sbjct: 185 PERLAELDGHVAETPEADDSEVLPRGVEAVLHH 87
>gb|CB878272.1|CB878272 HP07O19T HP Hordeum vulgare subsp. vulgare cDNA clone HP07O19
5-PRIME, mRNA sequence
Length = 595
Score = 131 bits (280), Expect = 9e-030
Identities = 52/90 (57%), Positives = 72/90 (80%)
Frame = +2 / +1
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
ISTSPGK++EAL+RLGAD F+VS+D E+M+AA TMDGI++TVSA ++P L+KP G
Sbjct: 325 ISTSPGKREEALERLGADEFLVSRDPEQMQAAVGTMDGILDTVSAWHPISPLFALMKPMG 504
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSC 391
+M+ VG PTKPLE+P + ++ G K +AG+C
Sbjct: 505 QMVFVGGPTKPLELPAYAIVPGGKGIAGNC 594
Score = 104 bits (221), Expect = 1e-021
Identities = 55/130 (42%), Positives = 59/130 (45%)
Frame = -1 / -3
Query: 390 QLPARVLFPMMRSKGGISRGLVGRPTMIILPLGLSRPMYGAKVRFADTVLMMPSMVXXXX 211
Q PA P + G S GLVG PT I P+G G ADTV MPSMV
Sbjct: 593 QFPAMPFPPGTMAYAGSSSGLVGPPTNTICPIGFISANSGEIGCHADTVSRMPSMVPTAA 414
Query: 210 XXXXXXXXXXXXXXXSRSSASCFFPGDVLMXXXXXXXXXXXXXXTWPRPPSPITPTCFPG 31
SRSSAS FPG+VLM TWPRPP P TP CFPG
Sbjct: 413 CICSGSRLTRNSSAPSRSSASSRFPGEVLMTVTRMPNALPNLTATWPRPPRPTTPRCFPG 234
Query: 30 TFRPWYFMGL 1
FRP +GL
Sbjct: 233 AFRPKRIIGL 204
>gb|BJ455039.1|BJ455039 BJ455039 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak15m16 3', mRNA sequence
Length = 446
Score = 96.4 bits (204), Expect(2) = 3e-028
Identities = 40/75 (53%), Positives = 53/75 (70%)
Frame = +2 / -1
Query: 335 LEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAMERLAKA 514
+ + P L +G +TL+GS GG +DTQEMIN A + + DIEL+ DY+N A+ERL
Sbjct: 296 IRVHPATLNLGARTLSGSVTGGTKDTQEMINFCAANKIYPDIELIKIDYINQALERLVNR 117
Query: 515 DVRYRFVIDIGNTLK 559
DVRYRFVIDI ++LK
Sbjct: 116 DVRYRFVIDIESSLK 72
Score = 49.2 bits (101), Expect(2) = 3e-028
Identities = 20/48 (41%), Positives = 31/48 (64%)
Frame = +2 / -1
Query: 182 VVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNGKMIMVGLP 325
VVS D ++M++ S++D I++T S + PY+ LLK G M +VG P
Sbjct: 446 VVSSDKKQMESLKSSLDFIVDTASGDHPFDPYLALLKVGGLMALVGFP 303
>gb|BI960471.1|BI960471 HVSMEn0024L10f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0024L10f, mRNA sequence
Length = 647
Score = 124 bits (265), Expect = 1e-027
Identities = 48/84 (57%), Positives = 67/84 (79%)
Frame = +2 / +2
Query: 323 PTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAMER 502
PTKPLE+P ++ G K +AG+C+GG+RD Q M+ AAKHG+TA++E++ DYVNTA+ER
Sbjct: 2 PTKPLELPGDAIVPGGKGIAGNCVGGIRDCQAMLEFAAKHGITAEVEVIKMDYVNTALER 181
Query: 503 LAKADVRYRFVIDIGNTLKNSE*S 574
+AK DVRYRFVIDI +L ++ *+
Sbjct: 182 VAKNDVRYRFVIDIAGSLGSTS*A 253
>gb|BQ761920.1|BQ761920 EBpi01_SQ005_D08_R pistil, 1 DPA, no treatment, cv Optic, EBpi01
Hordeum vulgare subsp. vulgare cDNA clone
EBpi01_SQ005_D08 5', mRNA sequence
Length = 383
Score = 124 bits (265), Expect = 1e-027
Identities = 50/58 (86%), Positives = 57/58 (98%)
Frame = +2 / +3
Query: 383 GSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAMERLAKADVRYRFVIDIGNTL 556
GSCIGGMRDTQEM+++AAKHGVTADIE+++A+YVNTAMERLAKADVRYRFVIDI NTL
Sbjct: 3 GSCIGGMRDTQEMLDLAAKHGVTADIEVISAEYVNTAMERLAKADVRYRFVIDIANTL 176
>gb|CB880767.1|CB880767 HM07M03w HM Hordeum vulgare subsp. vulgare cDNA clone HM07M03
3-PRIME, mRNA sequence
Length = 552
Score = 124 bits (265), Expect = 1e-027
Identities = 50/58 (86%), Positives = 57/58 (98%)
Frame = +2 / -3
Query: 383 GSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAMERLAKADVRYRFVIDIGNTL 556
GSCIGGMRDTQEM+++AAKHGVTADIE+++A+YVNTAMERLAKADVRYRFVIDI NTL
Sbjct: 550 GSCIGGMRDTQEMLDLAAKHGVTADIEVISAEYVNTAMERLAKADVRYRFVIDIANTL 377
>gb|BI957762.1|BI957762 HVSMEn0011B06f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0011B06f, mRNA sequence
Length = 1173
Score = 117 bits (250), Expect(2) = 3e-027
Identities = 54/111 (48%), Positives = 75/111 (67%)
Frame = +2 / +2
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
IS+SP K++EAL+RL ADAF+VS+DAE+MKAAA TMD II+TVSA L P + L+KP G
Sbjct: 200 ISSSPRKREEALERLCADAFLVSQDAEQMKAAAGTMDSIIDTVSAGHPLVPLLELMKPMG 379
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTA 454
M++VG P +PL + +I + LA + +G + D Q M++ TA
Sbjct: 380 HMVVVGAPNEPLALSA*AIIRRGRRLARNGVGNVHDCQAMLDFGGSTVSTA 532
Score = 84.0 bits (177), Expect(2) = 1e-023
Identities = 40/91 (43%), Positives = 49/91 (53%)
Frame = -3 / -3
Query: 427 VDHLLRVPHAADAAPGQGLVPNDEIKGRDLERLGGQADHDHLAVGLEQAHVRGQGEVRRH 248
V H L + H A A PGQ +D+ G + L +ADH H+ Q + RRH
Sbjct: 505 VQHRLAIVHVAHAVPGQAPAASDDSLG*QRQGLVRRADHHHMPHRFHQLQQWHERVPRRH 326
Query: 247 RVDDAVHGARSRLHFFGVLADDEGVGAKPVE 155
RVDDAVH RS H GVLAD E VGA+ +E
Sbjct: 325 RVDDAVHRPRSSFHLLGVLADQERVGAEALE 233
Score = 46.4 bits (95), Expect(2) = 1e-023
Identities = 19/35 (54%), Positives = 25/35 (71%)
Frame = -3 / -3
Query: 106 PERLGELDGDVAQASQPHHADVLPRHVQAVVLHGA 2
PERL ELDG VA+ + ++VLPR V+AV+ H A
Sbjct: 184 PERLAELDGHVAETPEAEDSEVLPRRVEAVLHHRA 80
Score = 24.9 bits (48), Expect(2) = 3e-027
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +2 / +1
Query: 437 KHGVTADIELVAADYV 484
KHGV D++ V DYV
Sbjct: 514 KHGVNRDVQDVKMDYV 561
>gb|BQ768475.1|BQ768475 EBro08_SQ011_G19_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ011_G19 5', mRNA sequence
Length = 421
Score = 85.4 bits (180), Expect(2) = 4e-027
Identities = 35/66 (53%), Positives = 49/66 (74%)
Frame = +2 / +1
Query: 212 AAASTMDGIINTVSANLTLAPYMGLLKPNGKMIMVGLPTKPLEIPPFDLIIGNKTLAGSC 391
AAA TMDGII+TVSA L P + L+KP G+M++VG P++PL +P + +I G K LAG+
Sbjct: 220 AAAGTMDGIIDTVSAGHPLVPLLELMKPMGQMVVVGAPSEPLALPAYAIIGGGKRLAGNG 399
Query: 392 IGGMRD 409
+G +RD
Sbjct: 400 VGSVRD 417
Score = 56.5 bits (117), Expect(2) = 4e-027
Identities = 23/34 (67%), Positives = 30/34 (88%)
Frame = +2 / +2
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAAS 223
IS+SP K++EAL+RLGADAF+VS+DAE+MK S
Sbjct: 35 ISSSPRKREEALERLGADAFLVSQDAEQMKVTLS 136
>gb|BI949722.1|BI949722 HVSMEl0015I24f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0015I24f, mRNA sequence
Length = 561
Score = 122 bits (260), Expect = 5e-027
Identities = 47/85 (55%), Positives = 66/85 (77%)
Frame = +2 / +1
Query: 299 GKMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAAD 478
G+M++VG P++PL +P + +I G K LAG+ +G +RD Q M++ A KHG+TAD+E+V D
Sbjct: 4 GQMVVVGAPSEPLALPAYAIIGGGKRLAGNGVGSVRDCQAMLDFAGKHGITADVEVVKMD 183
Query: 479 YVNTAMERLAKADVRYRFVIDIGNT 553
YVNTA+ERL K DVRYRFVID+ +
Sbjct: 184 YVNTAVERLEKNDVRYRFVIDVAGS 258
>gb|BQ662843.1|BQ662843 HS04G20u HS Hordeum vulgare subsp. vulgare cDNA clone HS04G20
3-PRIME, mRNA sequence
Length = 475
Score = 96.4 bits (204), Expect(2) = 1e-026
Identities = 40/75 (53%), Positives = 53/75 (70%)
Frame = +2 / -3
Query: 335 LEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADYVNTAMERLAKA 514
+ + P L +G +TL+GS GG +DTQEMIN A + + DIEL+ DY+N A+ERL
Sbjct: 332 IRVHPATLNLGARTLSGSVTGGTKDTQEMINFCAANKIYPDIELIKIDYINQALERLVNR 153
Query: 515 DVRYRFVIDIGNTLK 559
DVRYRFVIDI ++LK
Sbjct: 152 DVRYRFVIDIESSLK 108
Score = 43.7 bits (89), Expect(2) = 1e-026
Identities = 17/44 (38%), Positives = 28/44 (63%)
Frame = +2 / -3
Query: 194 DAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNGKMIMVGLP 325
D ++M++ S++D I++T S + PY+ LLK G M +VG P
Sbjct: 470 DKKQMESLKSSLDFIVDTASGDHPFDPYLALLKVGGLMALVGFP 339
>gb|BM368384.2|BM368384 EBed01_SQ002_P23_R endosperm, 6 DPA, no treatment, cv Optic, EBed01
Hordeum vulgare subsp. vulgare cDNA clone
EBed01_SQ002_P23 5', mRNA sequence
Length = 568
Score = 119 bits (255), Expect = 2e-026
Identities = 48/82 (58%), Positives = 66/82 (80%)
Frame = +2 / +3
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
ISTSPGK++EAL+RLGAD F+VS+D E+M+AA TMDGI++TVSA ++P L+KP G
Sbjct: 321 ISTSPGKREEALERLGADEFLVSRDPEQMQAAVGTMDGILDTVSAWHPISPLFALMKPMG 500
Query: 302 KMIMVGLPTKPLEIPPFDLIIG 367
+M+ VG PTKPLE+P + ++ G
Sbjct: 501 QMVFVGGPTKPLELPAYAIVPG 566
>gb|BG299988.1|BG299988 HVSMEa0022N15f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0022N15f, mRNA sequence
Length = 787
Score = 114 bits (244), Expect = 8e-025
Identities = 54/114 (47%), Positives = 76/114 (66%)
Frame = +2 / +3
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
IS+SP K++EAL+RLGADAF+VS+DAE+MKAAA TMDGII+TVSA L P + L+KP G
Sbjct: 432 ISSSPRKREEALERLGADAFLVSQDAEQMKAAAGTMDGIIDTVSAGHPLVPLLELMKPMG 611
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIE 463
+M+ V P +PL +P + L + + L G + M++ + +T D E
Sbjct: 612 QMVGVCPPNEPLALPTYPLHLRPEALVLERSGPPSQFKFMLDFPGEQCITPDGE 773
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 455,528,964
Number of Sequences: 312970
Number of extensions: 7272644
Number of successful extensions: 107432
Number of sequences better than 1.0e-020: 75
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 13581999925
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)