TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4005867.2.1
(1967 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI780849.2|BI780849 EBma05_SQ001_C24_R maternal, 12 DPA,... 471 e-132
gb|BI950935.1|BI950935 HVSMEl0023L12f Hordeum vulgare spike... 415 e-115
gb|BG415761.2|BG415761 HVSMEk0007L15f Hordeum vulgare testa... 219 4e-056
gb|BJ465418.1|BJ465418 BJ465418 K. Sato unpublished cDNA li... 218 1e-055
gb|BQ754059.1|BQ754059 EBca01_SQ002_L07_R carpel, pre-anthe... 160 2e-038
gb|CK122985.1|CK122985 BES1824101k13 BES1824 Hordeum vulgar... 110 5e-037
gb|BI956815.1|BI956815 HVSMEn0005H07f Hordeum vulgare rachi... 98 4e-034
gb|BI956859.1|BI956859 HVSMEn0005L01f Hordeum vulgare rachi... 98 1e-033
gb|CK122193.1|CK122193 BES1824101p15 BES1824 Hordeum vulgar... 110 1e-032
gb|BE422260.1|BE422260 HWM022cC.01r ITEC HWM Barley Leaf Li... 111 4e-028
gb|BM442480.2|BM442480 EBan01_SQ003_E21_R anther, yellow st... 121 2e-026
gb|CA029858.1|CA029858 HX05G15r HX Hordeum vulgare subsp. v... 76 5e-026
gb|BQ765469.1|BQ765469 EBro03_SQ007_C09_R root, 3 week, wat... 112 9e-026
gb|CB859122.1|CB859122 HI09G17w HI Hordeum vulgare subsp. v... 99 1e-025
gb|BQ765947.1|BQ765947 EBro03_SQ008_I17_R root, 3 week, wat... 102 2e-025
gb|BI954264.1|BI954264 HVSMEm0016O11f Hordeum vulgare green... 105 2e-025
gb|CB859989.1|CB859989 HI12E03w HI Hordeum vulgare subsp. v... 99 2e-024
gb|BJ468665.1|BJ468665 BJ468665 K. Sato unpublished cDNA li... 91 4e-022
gb|BM374246.2|BM374246 EBpi03_SQ003_B10_R pistil, 4 DPA, no... 89 2e-021
>gb|BI780849.2|BI780849 EBma05_SQ001_C24_R maternal, 12 DPA, no treatment, cv Optic, EBma05
Hordeum vulgare subsp. vulgare cDNA clone
EBma05_SQ001_C24 5', mRNA sequence
Length = 602
Score = 471 bits (1023), Expect = e-132
Identities = 189/200 (94%), Positives = 196/200 (98%)
Frame = +1 / +3
Query: 934 KQSRVTGRKSFADGITTMKTATFSVEASGFICKNMGFHNTAGAERHQAVALRVQGDLAAF 1113
KQSRVTG KSFADGITTMKTATFS+EA+GFICKNMGFHNTAGAE+HQAVALRVQGDL+AF
Sbjct: 3 KQSRVTGSKSFADGITTMKTATFSIEAAGFICKNMGFHNTAGAEKHQAVALRVQGDLSAF 182
Query: 1114 YNCRFDAFQDTLYVHARRQFFRNCVVSGTIDFIFGNSAAVFQNCLIITRRPMDNQQNSVT 1293
+NCRFDAFQDTLYVHARRQFFRNCV+SGTIDFIFGNSAAVFQNCLIITRRPMDNQQNSVT
Sbjct: 183 FNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIFGNSAAVFQNCLIITRRPMDNQQNSVT 362
Query: 1294 AHGRTDPNMKSGLVIQNCRLVPDQKLFPDRFKIPSYLGRPWKEFSRLVIMESTIADFVKP 1473
AHGRTDPNMKSGLVIQNCRLVPDQKLF DRFKIPSYLGRPWKEFSRLVIMES IADF+KP
Sbjct: 363 AHGRTDPNMKSGLVIQNCRLVPDQKLFVDRFKIPSYLGRPWKEFSRLVIMESQIADFIKP 542
Query: 1474 EGYMPWNGDFALKTLYYAEY 1533
EGYMPWNGDF LKTLYYAEY
Sbjct: 543 EGYMPWNGDFGLKTLYYAEY 602
Score = 421 bits (914), Expect = e-117
Identities = 178/200 (89%), Positives = 180/200 (90%)
Frame = -1 / -2
Query: 1532 YSA**SVLRAKSPFQGMYPSGLTKSAMVLSMMTRRENSFQGRPRYEGILKRSGNSFWSGT 1353
YSA**SVL KSPF GMYPSGL KSA+ LSMMT RENSF G PR +GILKRS SFWSGT
Sbjct: 601 YSA**SVLSPKSPFHGMYPSGLMKSAIWLSMMTSRENSFHGLPR*DGILKRSTKSFWSGT 422
Query: 1352 RRQFWMTSPDFMLGSVRPCAVTEFCWLSMGRRVMMRQFWNTAAELPKMKSMVPETTQLRK 1173
RRQFWMTSPDFMLGSVRPCAVTEFCWLSMGRRVMMRQFWNTAAELPKMKSMVPE TQLRK
Sbjct: 421 RRQFWMTSPDFMLGSVRPCAVTEFCWLSMGRRVMMRQFWNTAAELPKMKSMVPEMTQLRK 242
Query: 1172 NCRRACTYSVSWNASNRQL*NAARSPCTRSATAWCRSAPAVLWNPMFLQMNPDASTEKVA 993
NCRRACTYSVSWNASNRQL A RSPCT SATAWC SAPAVLWNPMFLQMNP AS EKVA
Sbjct: 241 NCRRACTYSVSWNASNRQLKKADRSPCTLSATAWCFSAPAVLWNPMFLQMNPAASMEKVA 62
Query: 992 VFMVVMPSAKLLRPVTRLCL 933
VFMVVMPSAKLL PVTRLCL
Sbjct: 61 VFMVVMPSAKLLLPVTRLCL 2
Score = 194 bits (418), Expect(2) = 8e-059
Identities = 87/115 (75%), Positives = 90/115 (78%)
Frame = -3 / -1
Query: 1491 PGHVPLWLDEVGDGALHDDEARELLPGAAQVRGDLEAVREQLLVGHQAAVLDDEPGLHVG 1312
P HVPL LDEVGD ALHDD+ RELLP AQV D EAV E+LLV HQAAVLDD+PGLHVG
Sbjct: 560 PRHVPLGLDEVGDLALHDDQPRELLPRPAQVGRDFEAVHEELLVRHQAAVLDDQPGLHVG 381
Query: 1311 VGAPXXXXXXXXXXHGPPRDDEAVLEHRRRVAEDEVDGAGDHAVAEELPPRVHVQ 1147
V AP HGPPRDDEAVLEH RRVAEDEVDGAGD AVAEELPP VHVQ
Sbjct: 380 VRAPVRGHRVLLVVHGPPRDDEAVLEHGRRVAEDEVDGAGDDAVAEELPPGVHVQ 216
Score = 144 bits (309), Expect(3) = 2e-043
Identities = 63/97 (64%), Positives = 66/97 (68%)
Frame = +3 / +2
Query: 1233 VPELPXXXXXXXGQPAELGDGARAHRPQHEVRARHPELPPGARPEAVPGPLQDXXXXXXX 1412
VPELP GQPAELGD ARAH PQHEVRA HPELPPGA PEA+ GPLQ+
Sbjct: 302 VPELPHHHAAAHGQPAELGDRARAHGPQHEVRAGHPELPPGAGPEALRGPLQNPVLPGQA 481
Query: 1413 XXXXXXXRHHGEHHRRLRQARGVHALERRLRPQDALL 1523
HHGE RRL QARGVHA+ERRLR QDALL
Sbjct: 482 VEGVLAAGHHGEPDRRLHQARGVHAVERRLRAQDALL 592
Score = 54.7 bits (113), Expect(2) = 8e-059
Identities = 22/25 (88%), Positives = 23/25 (92%)
Frame = -3 / -1
Query: 1092 HPERHRLVPLRAGRVVEPHVLADEP 1018
HP+RHRLV LRA RVVEPHVLADEP
Sbjct: 161 HPQRHRLVFLRARRVVEPHVLADEP 87
Score = 46.4 bits (95), Expect(3) = 2e-043
Identities = 20/23 (86%), Positives = 21/23 (91%)
Frame = +3 / +2
Query: 1110 VLQLPVRRVPGHAVRARAAAVLP 1178
+LQLPV RVPGHAVRAR AAVLP
Sbjct: 179 LLQLPV*RVPGHAVRARPAAVLP 247
Score = 24.9 bits (48), Expect(3) = 2e-043
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +3 / +2
Query: 1029 QEHGVPQH 1052
QEHGVPQH
Sbjct: 98 QEHGVPQH 121
>gb|BI950935.1|BI950935 HVSMEl0023L12f Hordeum vulgare spike EST library HVcDNA0012 (Fusarium
infected) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEl0023L12f, mRNA sequence
Length = 536
Score = 415 bits (901), Expect = e-115
Identities = 165/177 (93%), Positives = 170/177 (96%)
Frame = +1 / +2
Query: 1084 LRVQGDLAAFYNCRFDAFQDTLYVHARRQFFRNCVVSGTIDFIFGNSAAVFQNCLIITRR 1263
LRVQGDL+AF+NCRFDAFQDTLYVHARRQFFRNCV+SGTIDFIFGNSAAVFQNCLIITRR
Sbjct: 2 LRVQGDLSAFFNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIFGNSAAVFQNCLIITRR 181
Query: 1264 PMDNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKLFPDRFKIPSYLGRPWKEFSRLVIM 1443
PMDNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKLF DRFKIPSYLGRPWKEFSRLVIM
Sbjct: 182 PMDNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKLFVDRFKIPSYLGRPWKEFSRLVIM 361
Query: 1444 ESTIADFVKPEGYMPWNGDFALKTLYYAEYNNRGPGAGTSKRVNWPGFHVIGRKEAE 1614
ES IADF+KPEGYMPWNGDF LKTLYYAEY NRGPGAGTSKRV WPGF VI R+EAE
Sbjct: 362 ESQIADFIKPEGYMPWNGDFGLKTLYYAEYGNRGPGAGTSKRVTWPGFRVIARQEAE 532
Score = 358 bits (776), Expect = 6e-098
Identities = 153/177 (86%), Positives = 156/177 (88%)
Frame = -1 / -3
Query: 1613 SASFRPMTWKPGQLTLLLVPAPGPRLLYSA**SVLRAKSPFQGMYPSGLTKSAMVLSMMT 1434
SAS MT KPGQ+TLLLVPAPGPRL YSA**SVL KSPF GMYPSGL KSA+ LSMMT
Sbjct: 531 SASCLAMTRKPGQVTLLLVPAPGPRLPYSA**SVLSPKSPFHGMYPSGLMKSAIWLSMMT 352
Query: 1433 RRENSFQGRPRYEGILKRSGNSFWSGTRRQFWMTSPDFMLGSVRPCAVTEFCWLSMGRRV 1254
RENSF G PR +GILKRS SFWSGTRRQFWMTSPDFMLGSVRPCAVTEFCWLSMGRRV
Sbjct: 351 SRENSFHGLPR*DGILKRSTKSFWSGTRRQFWMTSPDFMLGSVRPCAVTEFCWLSMGRRV 172
Query: 1253 MMRQFWNTAAELPKMKSMVPETTQLRKNCRRACTYSVSWNASNRQL*NAARSPCTRS 1083
MMRQFWNTAAELPKMKSMVPE TQLRKNCRRACTYSVSWNASNRQL A RSPCT S
Sbjct: 171 MMRQFWNTAAELPKMKSMVPEMTQLRKNCRRACTYSVSWNASNRQLKKADRSPCTLS 1
Score = 194 bits (418), Expect(2) = 2e-051
Identities = 87/115 (75%), Positives = 90/115 (78%)
Frame = -3 / -2
Query: 1491 PGHVPLWLDEVGDGALHDDEARELLPGAAQVRGDLEAVREQLLVGHQAAVLDDEPGLHVG 1312
P HVPL LDEVGD ALHDD+ RELLP AQV D EAV E+LLV HQAAVLDD+PGLHVG
Sbjct: 409 PRHVPLGLDEVGDLALHDDQPRELLPRPAQVGRDFEAVHEELLVRHQAAVLDDQPGLHVG 230
Query: 1311 VGAPXXXXXXXXXXHGPPRDDEAVLEHRRRVAEDEVDGAGDHAVAEELPPRVHVQ 1147
V AP HGPPRDDEAVLEH RRVAEDEVDGAGD AVAEELPP VHVQ
Sbjct: 229 VRAPVRGHRVLLVVHGPPRDDEAVLEHGRRVAEDEVDGAGDDAVAEELPPGVHVQ 65
Score = 144 bits (309), Expect(2) = 2e-041
Identities = 63/97 (64%), Positives = 66/97 (68%)
Frame = +3 / +1
Query: 1233 VPELPXXXXXXXGQPAELGDGARAHRPQHEVRARHPELPPGARPEAVPGPLQDXXXXXXX 1412
VPELP GQPAELGD ARAH PQHEVRA HPELPPGA PEA+ GPLQ+
Sbjct: 151 VPELPHHHAAAHGQPAELGDRARAHGPQHEVRAGHPELPPGAGPEALRGPLQNPVLPGQA 330
Query: 1413 XXXXXXXRHHGEHHRRLRQARGVHALERRLRPQDALL 1523
HHGE RRL QARGVHA+ERRLR QDALL
Sbjct: 331 VEGVLAAGHHGEPDRRLHQARGVHAVERRLRAQDALL 441
Score = 46.4 bits (95), Expect(2) = 2e-041
Identities = 20/23 (86%), Positives = 21/23 (91%)
Frame = +3 / +1
Query: 1110 VLQLPVRRVPGHAVRARAAAVLP 1178
+LQLPV RVPGHAVRAR AAVLP
Sbjct: 28 LLQLPV*RVPGHAVRARPAAVLP 96
Score = 29.9 bits (59), Expect(2) = 2e-051
Identities = 12/17 (70%), Positives = 13/17 (76%)
Frame = -2 / -1
Query: 1609 PPSVR*RGSRAS*PSCW 1559
PP+ R* GS A *PSCW
Sbjct: 527 PPAWR*LGSPAR*PSCW 477
>gb|BG415761.2|BG415761 HVSMEk0007L15f Hordeum vulgare testa/pericarp EST library HVcDNA0013
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEk0007L15f, mRNA sequence
Length = 650
Score = 219 bits (473), Expect = 4e-056
Identities = 90/189 (47%), Positives = 117/189 (61%)
Frame = +1 / +2
Query: 751 KPNAIVAKDGSGQFKSIQQAVDAVPKGHQGRYVIYVKAGLYDEIVMVPKDKVNIFMYGDG 930
+P+ +VAKDGSG++ ++ AVDA P G + RYVIYVK G+Y E + V K K + + GDG
Sbjct: 71 QPDVVVAKDGSGKYTTVHAAVDAAPDGGKSRYVIYVKKGVYKENLEVGKKKRKLMIVGDG 250
Query: 931 PKQSRVTGRKSFADGITTMKTATFSVEASGFICKNMGFHNTAGAERHQAVALRVQGDLAA 1110
+ +TG ++ DG TT +AT +V A G I ++ NTA E H AVALRV D A
Sbjct: 251 MDATVITGSRNVVDGATTFNSATLAVAADGVILXDLRIENTAVPEMHHAVALRVSADRAV 430
Query: 1111 FYNCRFDAFQDTLYVHARRQFFRNCVVSGTIDFIFGNSAAVFQNCLIITRRPMDNQQNSV 1290
CR D +QDTLY H R F+R+C V GT+DF F N+ V Q CL+ RR Q+ V
Sbjct: 431 INRCRVDGYQDTLYAHQLRDFYRDCAVCGTVDFFFSNAGVVLQGCLLTERRAARGQKKRV 610
Query: 1291 TAHGRTDPN 1317
T HG TDPN
Sbjct: 611 TGHGMTDPN 637
Score = 136 bits (291), Expect = 5e-031
Identities = 81/190 (42%), Positives = 94/190 (49%)
Frame = -1 / -3
Query: 1322 FMLGSVRPCAVTEFCWLSMGRRVMMRQFWNTAAELPKMKSMVPETTQLRKNCRRACTYSV 1143
F GSV PC VT F RR + +Q +T L K KS VP+T Q R R C V
Sbjct: 642 FGFGSVMPCPVTRFF*PRAARRSVSKQP*STTPALLKKKSTVPQTAQSR*KSRSWCA*RV 463
Query: 1142 SWNASNRQL*NAARSPCTRSATAWCRSAPAVLWNPMFLQMNPDASTEKVAVFMVVMPSAK 963
SW S RQ ARS TR ATAWC S AV ++ P A+T +VA VV PS
Sbjct: 462 SW*PSTRQRLMTARSALTRRATAWCISGTAVFSILRSCRITPSAATARVAELKVVAPSTT 283
Query: 962 LLRPVTRLCLGPSPYMKMLTLSLGTMTISS*RPAFT*MTYLP*WPLGTASTACWMLLNCP 783
L PV + PSP + L T S P FT*+TY P G ASTA ++ P
Sbjct: 282 LRLPVITVASMPSPTIISLRFFFPTSRFSLYTPFFT*ITYRLLPPSGAASTAACTVVYLP 103
Query: 782 LPSLATIAFG 753
LPSLAT G
Sbjct: 102 LPSLATTTSG 73
>gb|BJ465418.1|BJ465418 BJ465418 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags39m02 5', mRNA sequence
Length = 528
Score = 218 bits (470), Expect = 1e-055
Identities = 88/171 (51%), Positives = 116/171 (67%)
Frame = +1 / +1
Query: 841 RYVIYVKAGLYDEIVMVPKDKVNIFMYGDGPKQSRVTGRKSFADGITTMKTATFSVEASG 1020
RYVIY+KAG Y E V V K+ N+ GDG ++ + + DG TT ++AT +V +
Sbjct: 10 RYVIYIKAGAYMENVEVGKNHKNLMFMGDGMGKTVIKASLNVVDGSTTFRSATVAVVGNN 189
Query: 1021 FICKNMGFHNTAGAERHQAVALRVQGDLAAFYNCRFDAFQDTLYVHARRQFFRNCVVSGT 1200
F+ +++ N AG +HQAVALRV DL+AFY C F +QDTLYVH+ RQFFR C + GT
Sbjct: 190 FLARDLTIENAAGPSKHQAVALRVGADLSAFYRCSFVGYQDTLYVHSLRQFFRECDIYGT 369
Query: 1201 IDFIFGNSAAVFQNCLIITRRPMDNQQNSVTAHGRTDPNMKSGLVIQNCRL 1353
IDF+FGNSAAV Q+C + RRP+ NQ N TA GRTDPN +G+ IQ C++
Sbjct: 370 IDFVFGNSAAVLQSCNLYARRPLPNQSNIYTAQGRTDPNQNTGISIQKCKV 522
Score = 125 bits (267), Expect(2) = 4e-030
Identities = 57/97 (58%), Positives = 60/97 (61%)
Frame = -1 / -2
Query: 1343 FWMTSPDFMLGSVRPCAVTEFCWLSMGRRVMMRQFWNTAAELPKMKSMVPETTQLRKNCR 1164
FWM P F LGSVRPCAV WL GRR Q W+TAAELPK KSMVP + RKN R
Sbjct: 512 FWMEMPVFWLGSVRPCAV*MLLWLGRGRRA*RLQLWSTAAELPKTKSMVP*MSHSRKN*R 333
Query: 1163 RACTYSVSWNASNRQL*NAARSPCTRSATAWCRSAPA 1053
R CT VSW + * A RS TRSATAWC PA
Sbjct: 332 RECT*RVSW*PTKLHR*KAERSAPTRSATAWCLDGPA 222
Score = 27.6 bits (54), Expect(2) = 4e-030
Identities = 20/57 (35%), Positives = 24/57 (42%)
Frame = -1 / -2
Query: 1010 STEKVAVFMVVMPSAKLLRPVTRLCLGPSPYMKMLTLSLGTMTISS*RPAFT*MTYL 840
+T VA VV PS L + + PSP T T S PA *+TYL
Sbjct: 179 TTATVAERKVVEPSTTLRLALITVLPIPSPMNMRFLWFFPTSTFSMYAPALM*ITYL 9
>gb|BQ754059.1|BQ754059 EBca01_SQ002_L07_R carpel, pre-anthesis, no treatment, cv Optic,
EBca01 Hordeum vulgare subsp. vulgare cDNA clone
EBca01_SQ002_L07 5', mRNA sequence
Length = 372
Score = 160 bits (344), Expect = 2e-038
Identities = 61/108 (56%), Positives = 80/108 (74%)
Frame = +1 / +2
Query: 1030 KNMGFHNTAGAERHQAVALRVQGDLAAFYNCRFDAFQDTLYVHARRQFFRNCVVSGTIDF 1209
++M F NTAG +HQAVALR DL+ FY C F+ QDTLY H+ RQF+R+C V+GT+DF
Sbjct: 5 RDMTFENTAGPAKHQAVALRCDSDLSVFYRCAFEGHQDTLYAHSLRQFYRDCRVAGTVDF 184
Query: 1210 IFGNSAAVFQNCLIITRRPMDNQQNSVTAHGRTDPNMKSGLVIQNCRL 1353
+FGN+AAVFQNCL++ R P+ Q+NSVTA GR + +M SG Q C +
Sbjct: 185 VFGNAAAVFQNCLLLARAPLPGQKNSVTAQGRFNASMNSGFRFQFCNV 328
Score = 126 bits (270), Expect = 4e-028
Identities = 58/106 (54%), Positives = 60/106 (56%)
Frame = -1 / -1
Query: 1346 QFWMTSPDFMLGSVRPCAVTEFCWLSMGRRVMMRQFWNTAAELPKMKSMVPETTQLRKNC 1167
Q W P+FM RP AVTEF W G R RQFWNTAA LPK KS VP T Q R N
Sbjct: 321 QNWKRKPEFMEALKRPWAVTEFFWPGSGARARRRQFWNTAAALPKTKSTVPATRQSR*NW 142
Query: 1166 RRACTYSVSWNASNRQL*NAARSPCTRSATAWCRSAPAVLWNPMFL 1029
R C YSVSW S * RS RSATAWC + PAV N M L
Sbjct: 141 RSECAYSVSWCPSKAHR*KTERSESQRSATAWCLAGPAVFSNVMSL 4
>gb|CK122985.1|CK122985 BES1824101k13 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010K131 5-PRIME, mRNA sequence
Length = 611
Score = 110 bits (235), Expect(3) = 5e-037
Identities = 45/94 (47%), Positives = 59/94 (62%)
Frame = +1 / +1
Query: 973 GITTMKTATFSVEASGFICKNMGFHNTAGAERHQAVALRVQGDLAAFYNCRFDAFQDTLY 1152
G++T TAT V A GF+ +++ NTAG + HQAVA R GD QDTLY
Sbjct: 142 GVSTFNTATVGVLADGFMARDLTIANTAGPDAHQAVAFRSTGDRTVLDTVELAGHQDTLY 321
Query: 1153 VHARRQFFRNCVVSGTIDFIFGNSAAVFQNCLII 1254
HA RQF+ C+V+GT+DFIFGNSAAV + ++
Sbjct: 322 AHAMRQFYTRCLVAGTVDFIFGNSAAVLHDSDVV 423
Score = 106 bits (227), Expect(3) = 2e-027
Identities = 49/95 (51%), Positives = 55/95 (57%)
Frame = -1 / -1
Query: 1238 WNTAAELPKMKSMVPETTQLRKNCRRACTYSVSWNASNRQL*NAARSPCTRSATAWCRSA 1059
W+TAAELPKMKS VP T NCR AC YSVSW ++ + + RSP R+ATAWC S
Sbjct: 407 WSTAAELPKMKSTVPATRHRV*NCRMACAYSVSWCPASSTVSSTVRSPVDRNATAWCASG 228
Query: 1058 PAVLWNPMFLQMNPDASTEKVAVFMVVMPSAKLLR 954
PAV M P AST VAV V P LR
Sbjct: 227 PAVFAIVRSRAMKPSASTPTVAVLKVETPGVSALR 123
Score = 44.6 bits (91), Expect(3) = 5e-037
Identities = 17/38 (44%), Positives = 26/38 (68%)
Frame = +1 / +1
Query: 841 RYVIYVKAGLYDEIVMVPKDKVNIFMYGDGPKQSRVTG 954
R+V+ VK G+Y E V VP +K N+ + GDG ++ +TG
Sbjct: 7 RFVVRVKEGVYKETVSVPWEKTNLVLVGDGMGKTVITG 120
Score = 39.1 bits (79), Expect(3) = 5e-037
Identities = 13/31 (41%), Positives = 22/31 (70%)
Frame = +1 / +1
Query: 1261 RPMDNQQNSVTAHGRTDPNMKSGLVIQNCRL 1353
RP + +++TA GRTDP +G+V++ CR+
Sbjct: 442 RPEXGETDALTAQGRTDPAQPTGIVLRGCRV 534
Score = 29.9 bits (59), Expect(3) = 2e-027
Identities = 15/36 (41%), Positives = 19/36 (52%)
Frame = -1 / -1
Query: 962 LLRPVTRLCLGPSPYMKMLTLSLGTMTISS*RPAFT 855
L PV + PSP L S GT+T+S P+FT
Sbjct: 128 LRSPVMTVLPMPSPTSTRLVFSHGTLTVSLYTPSFT 21
Score = 24.9 bits (48), Expect(3) = 2e-027
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = -1 / -1
Query: 1349 RQFWMTSPDFMLGSVRPCAVT 1287
RQ T P GSVRPCAV+
Sbjct: 530 RQPRSTIPVGCAGSVRPCAVS 468
>gb|BI956815.1|BI956815 HVSMEn0005H07f Hordeum vulgare rachis EST library HVcDNA0015 (normal)
Hordeum vulgare subsp. vulgare cDNA clone HVSMEn0005H07f,
mRNA sequence
Length = 877
Score = 98.2 bits (208), Expect(3) = 4e-034
Identities = 35/71 (49%), Positives = 50/71 (70%)
Frame = +1 / +3
Query: 1159 ARRQFFRNCVVSGTIDFIFGNSAAVFQNCLIITRRPMDNQQNSVTAHGRTDPNMKSGLVI 1338
+ RQF+R CV+SGT+DFI+GN+ AVFQ+CL++ R P+ + N +TA GR P+ SG
Sbjct: 6 SNRQFYRECVISGTVDFIYGNAKAVFQDCLLLARAPLKGKHNVITAQGRDGPDGSSGFSF 185
Query: 1339 QNCRLVPDQKL 1371
QNCR+ + L
Sbjct: 186 QNCRVTSQEDL 218
Score = 45.5 bits (93), Expect(3) = 4e-034
Identities = 15/33 (45%), Positives = 21/33 (63%)
Frame = +1 / +3
Query: 1396 SYLGRPWKEFSRLVIMESTIADFVKPEGYMPWN 1494
+Y GRPWK S +V M+S + V P G++ WN
Sbjct: 237 TYFGRPWKNHSHVVFMQSFLDTIVHPSGWVAWN 335
Score = 40.5 bits (82), Expect(3) = 4e-034
Identities = 14/22 (63%), Positives = 16/22 (72%)
Frame = +1 / +3
Query: 1513 TLYYAEYNNRGPGAGTSKRVNW 1578
T++Y EY N GPGA SKRV W
Sbjct: 360 TVFYGEYGNTGPGANLSKRVPW 425
>gb|BI956859.1|BI956859 HVSMEn0005L01f Hordeum vulgare rachis EST library HVcDNA0015 (normal)
Hordeum vulgare subsp. vulgare cDNA clone HVSMEn0005L01f,
mRNA sequence
Length = 867
Score = 98.2 bits (208), Expect(3) = 1e-033
Identities = 35/71 (49%), Positives = 50/71 (70%)
Frame = +1 / +3
Query: 1159 ARRQFFRNCVVSGTIDFIFGNSAAVFQNCLIITRRPMDNQQNSVTAHGRTDPNMKSGLVI 1338
+ RQF+R CV+SGT+DFI+GN+ AVFQ+CL++ R P+ + N +TA GR P+ SG
Sbjct: 6 SNRQFYRECVISGTVDFIYGNAKAVFQDCLLLARAPLKGKHNVITAQGRDGPDGSSGFSF 185
Query: 1339 QNCRLVPDQKL 1371
QNCR+ + L
Sbjct: 186 QNCRVTSQEDL 218
Score = 45.5 bits (93), Expect(3) = 1e-033
Identities = 15/33 (45%), Positives = 21/33 (63%)
Frame = +1 / +3
Query: 1396 SYLGRPWKEFSRLVIMESTIADFVKPEGYMPWN 1494
+Y GRPWK S +V M+S + V P G++ WN
Sbjct: 237 TYFGRPWKNHSHVVFMQSFLDTIVHPSGWVAWN 335
Score = 39.1 bits (79), Expect(3) = 1e-033
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = +1 / +3
Query: 1513 TLYYAEYNNRGPGAGTSKRVNW 1578
T++Y EY N GPGA +KRV W
Sbjct: 360 TVFYGEYGNTGPGANLTKRVPW 425
>gb|CK122193.1|CK122193 BES1824101p15 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010P151 5-PRIME, mRNA sequence
Length = 641
Score = 110 bits (235), Expect(3) = 1e-032
Identities = 45/94 (47%), Positives = 59/94 (62%)
Frame = +1 / +1
Query: 973 GITTMKTATFSVEASGFICKNMGFHNTAGAERHQAVALRVQGDLAAFYNCRFDAFQDTLY 1152
G++T TAT V A GF+ +++ NTAG + HQAVA R GD QDTLY
Sbjct: 142 GVSTFNTATVGVLADGFMARDLTIANTAGPDAHQAVAFRSTGDRTVLDTVELAGHQDTLY 321
Query: 1153 VHARRQFFRNCVVSGTIDFIFGNSAAVFQNCLII 1254
HA RQF+ C+V+GT+DFIFGNSAAV + ++
Sbjct: 322 AHAMRQFYTRCLVAGTVDFIFGNSAAVLHDSDVV 423
Score = 106 bits (227), Expect(3) = 3e-027
Identities = 49/95 (51%), Positives = 55/95 (57%)
Frame = -1 / -1
Query: 1238 WNTAAELPKMKSMVPETTQLRKNCRRACTYSVSWNASNRQL*NAARSPCTRSATAWCRSA 1059
W+TAAELPKMKS VP T NCR AC YSVSW ++ + + RSP R+ATAWC S
Sbjct: 407 WSTAAELPKMKSTVPATRHRV*NCRMACAYSVSWCPASSTVSSTVRSPVDRNATAWCASG 228
Query: 1058 PAVLWNPMFLQMNPDASTEKVAVFMVVMPSAKLLR 954
PAV M P AST VAV V P LR
Sbjct: 227 PAVFAIVRSRAMKPSASTPTVAVLKVETPGVSALR 123
Score = 44.6 bits (91), Expect(3) = 1e-032
Identities = 17/38 (44%), Positives = 26/38 (68%)
Frame = +1 / +1
Query: 841 RYVIYVKAGLYDEIVMVPKDKVNIFMYGDGPKQSRVTG 954
R+V+ VK G+Y E V VP +K N+ + GDG ++ +TG
Sbjct: 7 RFVVRVKEGVYKETVSVPWEKTNLVLVGDGMGKTVITG 120
Score = 29.9 bits (59), Expect(3) = 1e-032
Identities = 10/13 (76%), Positives = 12/13 (92%)
Frame = +1 / +2
Query: 1399 YLGRPWKEFSRLV 1437
+LGRPWKE+SR V
Sbjct: 593 FLGRPWKEYSRPV 631
Score = 29.9 bits (59), Expect(3) = 3e-027
Identities = 15/36 (41%), Positives = 19/36 (52%)
Frame = -1 / -1
Query: 962 LLRPVTRLCLGPSPYMKMLTLSLGTMTISS*RPAFT 855
L PV + PSP L S GT+T+S P+FT
Sbjct: 128 LRSPVMTVLPMPSPTSTRLVFSHGTLTVSLYTPSFT 21
Score = 24.0 bits (46), Expect(3) = 3e-027
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = -1 / -1
Query: 1334 TSPDFMLGSVRPCAVT 1287
T P GSVRPCAV+
Sbjct: 515 TIPVGCAGSVRPCAVS 468
>gb|BE422260.1|BE422260 HWM022cC.01r ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM022cC.01, mRNA sequence
Length = 1239
Score = 111 bits (236), Expect(2) = 4e-028
Identities = 41/67 (61%), Positives = 49/67 (73%)
Frame = +1 / +3
Query: 1396 SYLGRPWKEFSRLVIMESTIADFVKPEGYMPWNGDFALKTLYYAEYNNRGPGAGTSKRVN 1575
SYLGRPWK++SR V M+S + V P G++ W+G FAL TLYY EY N GPGAGTS RV
Sbjct: 117 SYLGRPWKQYSRTVFMQSELDSVVNPAGWLAWDGTFALDTLYYGEYQNTGPGAGTSGRVT 296
Query: 1576 WPGFHVI 1596
W G+ VI
Sbjct: 297 WKGYRVI 317
Score = 35.0 bits (70), Expect(2) = 4e-028
Identities = 14/32 (43%), Positives = 16/32 (50%)
Frame = +1 / +3
Query: 1591 VIGRKEAEPFTAGPFIDGAMWLKYTGAPHILG 1686
+ EA FT G FIDG +WL T P G
Sbjct: 315 ITSASEASTFTVGSFIDGDVWLAGTSIPFSAG 410
>gb|BM442480.2|BM442480 EBan01_SQ003_E21_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_E21 5', mRNA sequence
Length = 230
Score = 121 bits (258), Expect = 2e-026
Identities = 50/64 (78%), Positives = 60/64 (93%)
Frame = +2 / +2
Query: 137 LPTSGKSVKSLCAPTLYKESCEKTLSQATNGTENPKEVFHSVAKVALESVQTAVEQSKSI 316
L TSGKSVKSLCAPTLYKESCEKTL+ A+NGTENPK+VF +VAK A+ES+++AVE+S+SI
Sbjct: 8 LATSGKSVKSLCAPTLYKESCEKTLTSASNGTENPKDVFATVAKSAMESIKSAVERSRSI 187
Query: 317 GEAK 328
GEAK
Sbjct: 188 GEAK 199
>gb|CA029858.1|CA029858 HX05G15r HX Hordeum vulgare subsp. vulgare cDNA clone HX05G15
5-PRIME, mRNA sequence
Length = 638
Score = 76.2 bits (160), Expect(3) = 5e-026
Identities = 27/63 (42%), Positives = 40/63 (63%)
Frame = +1 / +1
Query: 1057 GAERHQAVALRVQGDLAAFYNCRFDAFQDTLYVHARRQFFRNCVVSGTIDFIFGNSAAVF 1236
G VALR+ D AAF C F QDTLY H R ++++C + G++DFIFGN+ +++
Sbjct: 4 GRAGEAGVALRISADNAAFVGCNFLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLY 183
Query: 1237 QNC 1245
+ C
Sbjct: 184 EGC 192
Score = 58.3 bits (121), Expect(3) = 5e-026
Identities = 22/60 (36%), Positives = 33/60 (55%)
Frame = +1 / +1
Query: 1399 YLGRPWKEFSRLVIMESTIADFVKPEGYMPWNGDFALKTLYYAEYNNRGPGAGTSKRVNW 1578
YLGR W FSR+V + + + + P G+ W T++Y +Y GPGA + RV+W
Sbjct: 310 YLGRAWGTFSRVVFAYTYMDNIIIPRGWYNWGDPTREMTVFYGQYKCSGPGANHAGRVDW 489
Score = 28.1 bits (55), Expect(3) = 5e-026
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = +1 / +1
Query: 1603 KEAEPFTAGPFIDGAMWLK 1659
+EA+PF + FIDG WL+
Sbjct: 508 EEAKPFISLSFIDGLEWLR 564
>gb|BQ765469.1|BQ765469 EBro03_SQ007_C09_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ007_C09 5', mRNA sequence
Length = 417
Score = 112 bits (239), Expect(2) = 9e-026
Identities = 40/61 (65%), Positives = 50/61 (81%)
Frame = +1 / +2
Query: 1414 WKEFSRLVIMESTIADFVKPEGYMPWNGDFALKTLYYAEYNNRGPGAGTSKRVNWPGFHV 1593
WK +SR VIM+S +A V P G+MPW+GDFAL TLYYAEY+N G G+ TS+RVNWPG+HV
Sbjct: 2 WKMYSRTVIMQSDVAGIVDPAGWMPWSGDFALSTLYYAEYDNSGAGSDTSRRVNWPGYHV 181
Query: 1594 I 1596
+
Sbjct: 182 L 184
Score = 25.8 bits (50), Expect(2) = 9e-026
Identities = 10/27 (37%), Positives = 13/27 (48%)
Frame = +1 / +2
Query: 1606 EAEPFTAGPFIDGAMWLKYTGAPHILG 1686
+A FT + G WL TG P +G
Sbjct: 197 DAGNFTVANMVLGDFWLPQTGVPFTVG 277
>gb|CB859122.1|CB859122 HI09G17w HI Hordeum vulgare subsp. vulgare cDNA clone HI09G17
3-PRIME, mRNA sequence
Length = 565
Score = 98.7 bits (209), Expect(2) = 1e-025
Identities = 35/56 (62%), Positives = 45/56 (80%)
Frame = +1 / -1
Query: 1399 YLGRPWKEFSRLVIMESTIADFVKPEGYMPWNGDFALKTLYYAEYNNRGPGAGTSK 1566
+LGRPWKE+SR V + T+A+ V+P G+MPW+GDFAL TLYY EY + GPGAG S+
Sbjct: 376 FLGRPWKEYSRTVFVGCTLAEIVQPAGWMPWSGDFALDTLYYGEYGSAGPGAGRSR 209
Score = 39.1 bits (79), Expect(2) = 1e-025
Identities = 13/31 (41%), Positives = 22/31 (70%)
Frame = +1 / -1
Query: 1261 RPMDNQQNSVTAHGRTDPNMKSGLVIQNCRL 1353
RP + +++TA GRTDP +G+V++ CR+
Sbjct: 526 RPEKGETDALTAQGRTDPAQPTGIVLRGCRV 434
>gb|BQ765947.1|BQ765947 EBro03_SQ008_I17_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ008_I17 5', mRNA sequence
Length = 723
Score = 102 bits (217), Expect(3) = 2e-025
Identities = 41/82 (50%), Positives = 55/82 (67%)
Frame = +1 / +3
Query: 1006 VEASGFICKNMGFHNTAGAERHQAVALRVQGDLAAFYNCRFDAFQDTLYVHARRQFFRNC 1185
VE FI +N+ F N+A QAVA+RV D AFY+CRF +QDTLY+H +Q+ R+C
Sbjct: 9 VEGEDFIAENITFQNSAPQGSGQAVAVRVTADKCAFYSCRFLGWQDTLYLHYGKQYLRDC 188
Query: 1186 VVSGTIDFIFGNSAAVFQNCLI 1251
+ G DFIFGNS A+ ++C I
Sbjct: 189 YIEGNCDFIFGNSIALLEHCHI 254
Score = 36.8 bits (74), Expect(3) = 2e-025
Identities = 17/50 (34%), Positives = 25/50 (50%)
Frame = +1 / +1
Query: 1429 RLVIMESTIADFVKPEGYMPWNGDFALKTLYYAEYNNRGPGAGTSKRVNW 1578
R+V + + VK G+ W+ +T + EY GPG+ TS RV W
Sbjct: 400 RVVFAHTFMDRCVKATGWHNWDKSENERTACFYEYRCSGPGSRTSSRVAW 549
Score = 21.7 bits (41), Expect(3) = 2e-025
Identities = 6/11 (54%), Positives = 8/11 (72%)
Frame = +1 / +3
Query: 1399 YLGRPWKEFSR 1431
+LGRPW F +
Sbjct: 369 FLGRPWGPFGK 401
>gb|BI954264.1|BI954264 HVSMEm0016O11f Hordeum vulgare green seedling EST library HVcDNA0014
(Blumeria infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEm0016O11f, mRNA sequence
Length = 674
Score = 105 bits (224), Expect(2) = 2e-025
Identities = 44/93 (47%), Positives = 58/93 (62%)
Frame = +1 / +2
Query: 973 GITTMKTATFSVEASGFICKNMGFHNTAGAERHQAVALRVQGDLAAFYNCRFDAFQDTLY 1152
G T T VE FI +N+ F N+A QAVA+RV D AFY+CRF +QDTLY
Sbjct: 191 GTGTFGCGTVIVEGEDFIAENITFQNSAPQGSGQAVAVRVTADKCAFYSCRFLGWQDTLY 370
Query: 1153 VHARRQFFRNCVVSGTIDFIFGNSAAVFQNCLI 1251
+H +Q+ R+C + G DFIFGNS A+ ++C I
Sbjct: 371 LHYGKQYLRDCYIEGNCDFIFGNSIALLEHCHI 469
Score = 31.3 bits (62), Expect(2) = 2e-025
Identities = 16/35 (45%), Positives = 21/35 (60%)
Frame = +1 / +2
Query: 820 VPKGHQGRYVIYVKAGLYDEIVMVPKDKVNIFMYG 924
VP G++ R VI + G+Y E V VPK K I + G
Sbjct: 8 VPLGNRERTVIRLAPGVYREPVYVPKTKNFITLAG 112
>gb|CB859989.1|CB859989 HI12E03w HI Hordeum vulgare subsp. vulgare cDNA clone HI12E03
3-PRIME, mRNA sequence
Length = 521
Score = 98.7 bits (209), Expect(2) = 2e-024
Identities = 35/56 (62%), Positives = 45/56 (80%)
Frame = +1 / -1
Query: 1399 YLGRPWKEFSRLVIMESTIADFVKPEGYMPWNGDFALKTLYYAEYNNRGPGAGTSK 1566
+LGRPWKE+SR V + T+A+ V+P G+MPW+GDFAL TLYY EY + GPGAG S+
Sbjct: 377 FLGRPWKEYSRTVFVGCTLAEIVQPAGWMPWSGDFALDTLYYGEYGSAGPGAGRSR 210
Score = 35.0 bits (70), Expect(2) = 2e-024
Identities = 11/26 (42%), Positives = 20/26 (76%)
Frame = +1 / -1
Query: 1276 QQNSVTAHGRTDPNMKSGLVIQNCRL 1353
+ +++TA GRTDP +G+V++ CR+
Sbjct: 512 ETDALTAQGRTDPAQPTGIVLRGCRV 435
>gb|BJ468665.1|BJ468665 BJ468665 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags39m02 3', mRNA sequence
Length = 606
Score = 90.9 bits (192), Expect(2) = 4e-022
Identities = 34/60 (56%), Positives = 42/60 (70%)
Frame = +1 / -3
Query: 1417 KEFSRLVIMESTIADFVKPEGYMPWNGDFALKTLYYAEYNNRGPGAGTSKRVNWPGFHVI 1596
K++SR V M+S + V P G++ W+G FAL TLYY EY N GPGAGTS RV W G+ VI
Sbjct: 604 KQYSRTVFMQSELDSVVNPAGWLAWDGTFALDTLYYGEYQNTGPGAGTSGRVTWKGYRVI 425
Score = 35.0 bits (70), Expect(2) = 4e-022
Identities = 14/32 (43%), Positives = 16/32 (50%)
Frame = +1 / -3
Query: 1591 VIGRKEAEPFTAGPFIDGAMWLKYTGAPHILG 1686
+ EA FT G FIDG +WL T P G
Sbjct: 427 ITSASEASTFTVGSFIDGDVWLAGTSIPFSAG 332
>gb|BM374246.2|BM374246 EBpi03_SQ003_B10_R pistil, 4 DPA, no treatment, cv Optic, EBpi03
Hordeum vulgare subsp. vulgare cDNA clone
EBpi03_SQ003_B10 5', mRNA sequence
Length = 449
Score = 88.6 bits (187), Expect(2) = 2e-021
Identities = 33/59 (55%), Positives = 41/59 (69%)
Frame = +1 / +2
Query: 1420 EFSRLVIMESTIADFVKPEGYMPWNGDFALKTLYYAEYNNRGPGAGTSKRVNWPGFHVI 1596
++SR V M+S + V P G++ W+G FAL TLYY EY N GPGAGTS RV W G+ VI
Sbjct: 2 QYSRTVFMQSELDSVVNPAGWLAWDGTFALDTLYYGEYQNTGPGAGTSGRVTWKGYRVI 178
Score = 35.0 bits (70), Expect(2) = 2e-021
Identities = 14/32 (43%), Positives = 16/32 (50%)
Frame = +1 / +2
Query: 1591 VIGRKEAEPFTAGPFIDGAMWLKYTGAPHILG 1686
+ EA FT G FIDG +WL T P G
Sbjct: 176 ITSASEASTFTVGSFIDGDVWLAGTSIPFSAG 271
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 812,449,866
Number of Sequences: 312970
Number of extensions: 14043146
Number of successful extensions: 207036
Number of sequences better than 1.0e-020: 25
length of database: 58,378,179
effective HSP length: 54
effective length of database: 41,477,799
effective search space used: 24928157199
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)