TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3995289.2.1
(559 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF621150.3|BF621150 HVSMEa0004J06f Hordeum vulgare seedl... 152 1e-060
gb|BF623053.2|BF623053 HVSMEa0012M22f Hordeum vulgare seedl... 151 2e-055
gb|BI953372.1|BI953372 HVSMEm0012F09f Hordeum vulgare green... 150 7e-053
gb|CA030254.1|CA030254 HX06I12r HX Hordeum vulgare subsp. v... 121 9e-049
gb|BF628008.2|BF628008 HVSMEb0002J11f Hordeum vulgare seedl... 147 2e-047
gb|BQ763476.1|BQ763476 EBro02_SQ008_B17_R root, 3 week, hyd... 162 2e-041
gb|BF259970.1|BF259970 HVSMEf0020M03f Hordeum vulgare seedl... 101 4e-031
gb|BE060220.1|BE060220 HVSMEg0011K01f Hordeum vulgare pre-a... 121 3e-027
gb|BI957153.1|BI957153 HVSMEn0007M22f Hordeum vulgare rachi... 121 3e-027
gb|BF258425.1|BF258425 HVSMEf0015L01f Hordeum vulgare seedl... 92 2e-026
gb|BU996125.1|BU996125 HM12I08r HM Hordeum vulgare subsp. v... 79 2e-023
gb|CA021326.1|CA021326 HZ39M08r HZ Hordeum vulgare subsp. v... 73 4e-023
gb|CA014589.1|CA014589 HT11M09r HT Hordeum vulgare subsp. v... 104 5e-022
gb|BM443422.2|BM443422 EBro02_SQ005_B06_R root, 3 week, hyd... 100 6e-021
>gb|BF621150.3|BF621150 HVSMEa0004J06f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0004J06f, mRNA sequence
Length = 735
Score = 152 bits (326), Expect(2) = 1e-060
Identities = 69/112 (61%), Positives = 82/112 (73%)
Frame = +1 / +3
Query: 58 RKDRPQLTLNFVVQFFFLALCGITANQGFYLLGLDNTSPTFASAIQNSVPAITFAMAAAL 237
+KDRP LTL+ + +FF LAL GITANQGFYLLGL + SPT+ASAIQN VPAITF +AA L
Sbjct: 33 KKDRPPLTLSLLAEFFVLALIGITANQGFYLLGLYHLSPTYASAIQNMVPAITFVLAAVL 212
Query: 238 RIEKVRLDRRDGVAKVAGTLACVAXASVITLYKGPTIFXXXXXXXLELASTS 393
R+E+V L RR GVAKV GT+ V A+VITLYKG +F L+ TS
Sbjct: 213 RLEQVDLGRRHGVAKVVGTVVSVGGATVITLYKGLPLFSHNLHVQAVLSWTS 368
Score = 100 bits (214), Expect(2) = 1e-060
Identities = 34/46 (73%), Positives = 42/46 (91%)
Frame = +1 / +3
Query: 415 NWTLGCVYLIGHCLSWSGWLVLQAPVLKKYPARLAVTSYTCFFGVI 552
NWTLGCV+++GHCLSWSGW+VLQ PVLK+YPARL+V S TC FG++
Sbjct: 384 NWTLGCVFILGHCLSWSGWMVLQVPVLKRYPARLSVISLTCVFGLL 521
Score = 91.8 bits (194), Expect(2) = 5e-027
Identities = 47/96 (48%), Positives = 54/96 (56%)
Frame = -3 / -3
Query: 338 PL*SVMTDAXATHASVPATLXXXXXXXXXXXXXXXXXAMAKVMAGTEFWMADANVGDVLS 159
PL*SV+T A T +VP TL A KVMAGT FW+ADA VGD
Sbjct: 313 PL*SVITVAPPTLTTVPTTLATPCRRPRSTCSSRSTAASTKVMAGTMFWIADA*VGDRWY 134
Query: 158 RPRR*NPWFAVMPQSARKKNWTTKLRVSCGLSFLQQ 51
P R*NPWFAV+P SA KN + +VS GLSF +
Sbjct: 133 SPSR*NPWFAVIPMSASTKNSASSDKVSGGLSFFSR 26
Score = 48.7 bits (100), Expect(2) = 5e-027
Identities = 23/44 (52%), Positives = 26/44 (59%)
Frame = -3 / -3
Query: 548 TPKKQV*EVTASRAGYFFSTGACSTSQPDHDRQCPIRYTXPSVQ 417
+PK QV E+T S AGY TG ST PD +RQ P T P VQ
Sbjct: 517 SPKTQVSEMTDSLAGYRLRTGTWSTIHPDQERQWPRMKTQPRVQ 386
>gb|BF623053.2|BF623053 HVSMEa0012M22f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0012M22f, mRNA sequence
Length = 833
Score = 151 bits (324), Expect(2) = 2e-055
Identities = 66/98 (67%), Positives = 78/98 (79%)
Frame = +1 / +2
Query: 58 RKDRPQLTLNFVVQFFFLALCGITANQGFYLLGLDNTSPTFASAIQNSVPAITFAMAAAL 237
+KDRP LTL+ + +FF LAL GITANQGFYLLGL + SPT+ASAIQN VPAITF +AA L
Sbjct: 287 KKDRPPLTLSLLAEFFVLALIGITANQGFYLLGLYHLSPTYASAIQNMVPAITFVLAAVL 466
Query: 238 RIEKVRLDRRDGVAKVAGTLACVAXASVITLYKGPTIF 351
R+E+V L RR GVAKV GT+ V A+VITLYKG +F
Sbjct: 467 RLEQVDLGRRHGVAKVVGTVVSVGGATVITLYKGLPLF 580
Score = 84.0 bits (177), Expect(2) = 2e-055
Identities = 30/46 (65%), Positives = 37/46 (80%)
Frame = +1 / +2
Query: 415 NWTLGCVYLIGHCLSWSGWLVLQAPVLKKYPARLAVTSYTCFFGVI 552
NWTLGCV+++GHCLS SGW+VL PVLK+YPARL S TC F ++
Sbjct: 638 NWTLGCVFILGHCLSCSGWMVLPVPVLKRYPARLFGISLTCVFRLL 775
>gb|BI953372.1|BI953372 HVSMEm0012F09f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0012F09f, mRNA sequence
Length = 831
Score = 150 bits (323), Expect(2) = 7e-053
Identities = 68/112 (60%), Positives = 82/112 (73%)
Frame = +1 / +1
Query: 58 RKDRPQLTLNFVVQFFFLALCGITANQGFYLLGLDNTSPTFASAIQNSVPAITFAMAAAL 237
+KDRP LTL+ + +FF LAL GITANQGFYLLG+ + SPT+ASAIQN VPAITF +AA L
Sbjct: 55 KKDRPPLTLSLLAEFFVLALIGITANQGFYLLGVCHLSPTYASAIQNMVPAITFVLAAVL 234
Query: 238 RIEKVRLDRRDGVAKVAGTLACVAXASVITLYKGPTIFXXXXXXXLELASTS 393
R+E+V L RR GVAKV GT+ V A+VITLYKG +F L+ TS
Sbjct: 235 RLEQVDLGRRHGVAKVVGTVVSVCGATVITLYKGLPLFSHNLHVQAVLSWTS 390
Score = 92.3 bits (195), Expect(2) = 3e-024
Identities = 47/96 (48%), Positives = 54/96 (56%)
Frame = -3 / -2
Query: 338 PL*SVMTDAXATHASVPATLXXXXXXXXXXXXXXXXXAMAKVMAGTEFWMADANVGDVLS 159
PL*SV+T A T +VP TL A KVMAGT FW+ADA VGD
Sbjct: 335 PL*SVITVAPQTLTTVPTTLATPCRRPRSTCSSRSTAASTKVMAGTMFWIADA*VGDRWH 156
Query: 158 RPRR*NPWFAVMPQSARKKNWTTKLRVSCGLSFLQQ 51
P R*NPWFAV+P SA KN + +VS GLSF +
Sbjct: 155 TPSR*NPWFAVIPMSASTKNSASSDKVSGGLSFFSR 48
Score = 76.2 bits (160), Expect(2) = 7e-053
Identities = 26/44 (59%), Positives = 33/44 (75%)
Frame = +1 / +1
Query: 415 NWTLGCVYLIGHCLSWSGWLVLQAPVLKKYPARLAVTSYTCFFG 546
NWTLGCV+++GHCLSWSGW+VLQ P ++ PARL+ T G
Sbjct: 406 NWTLGCVFILGHCLSWSGWMVLQGPGAQRDPARLSALEVTWVLG 537
Score = 39.1 bits (79), Expect(2) = 3e-024
Identities = 17/34 (50%), Positives = 21/34 (61%)
Frame = -2 / -1
Query: 516 QPCRVLLQHGSLQHQPAGPRQAVPDQVHAXQRPV 415
QP RV L G L+H P+ P +AV + A QRPV
Sbjct: 507 QPRRVPLSAGPLEHHPSRPGEAVAEYEDAAQRPV 406
>gb|CA030254.1|CA030254 HX06I12r HX Hordeum vulgare subsp. vulgare cDNA clone HX06I12
5-PRIME, mRNA sequence
Length = 651
Score = 121 bits (259), Expect(2) = 9e-049
Identities = 45/47 (95%), Positives = 47/47 (100%)
Frame = +1 / +1
Query: 412 KNWTLGCVYLIGHCLSWSGWLVLQAPVLKKYPARLAVTSYTCFFGVI 552
KNWTLGCVYLIGHCLSWSGWLVLQAPVLKKYPA+L+VTSYTCFFGVI
Sbjct: 202 KNWTLGCVYLIGHCLSWSGWLVLQAPVLKKYPAKLSVTSYTCFFGVI 342
Score = 99.1 bits (210), Expect(2) = 1e-032
Identities = 40/46 (86%), Positives = 41/46 (89%)
Frame = -3 / -2
Query: 551 MTPKKQV*EVTASRAGYFFSTGACSTSQPDHDRQCPIRYTXPSVQF 414
MTPKKQV*EVT S AGYF STGACSTS PDHDRQCP+RYT PSVQF
Sbjct: 341 MTPKKQV*EVTDSFAGYFLSTGACSTSHPDHDRQCPMRYTHPSVQF 204
Score = 91.8 bits (194), Expect(2) = 9e-049
Identities = 40/43 (93%), Positives = 41/43 (95%)
Frame = +1 / +2
Query: 223 MAAALRIEKVRLDRRDGVAKVAGTLACVAXASVITLYKGPTIF 351
MAAALRIEKVRLDRRDGVAKV GTLACVA ASVITLY+GPTIF
Sbjct: 5 MAAALRIEKVRLDRRDGVAKVVGTLACVAGASVITLYQGPTIF 133
Score = 85.4 bits (180), Expect(2) = 2e-023
Identities = 36/47 (76%), Positives = 39/47 (82%)
Frame = -2 / -1
Query: 552 NDAEEAGVGGDGQPCRVLLQHGSLQHQPAGPRQAVPDQVHAXQRPVL 412
+DAEEAGVGGD Q VLL+HG LQHQP PRQAVPD+VHA QRPVL
Sbjct: 342 DDAEEAGVGGDRQLRGVLLEHGCLQHQPPRPRQAVPDEVHAPQRPVL 202
Score = 60.2 bits (125), Expect(2) = 1e-032
Identities = 24/33 (72%), Positives = 27/33 (81%)
Frame = -2 / -3
Query: 333 VERDDGCXRHARQRARHLGYAVAAVQPDLLDPQ 235
VERDDGC RHARQ A HL +AVA V+P LLDP+
Sbjct: 115 VERDDGCPRHARQGAHHLCHAVAPVEPHLLDPE 17
Score = 43.2 bits (88), Expect(2) = 2e-023
Identities = 19/23 (82%), Positives = 19/23 (82%)
Frame = -3 / -1
Query: 350 KMVGPL*SVMTDAXATHASVPAT 282
KMVGP *SVMTDA ATHA VP T
Sbjct: 132 KMVGPW*SVMTDAPATHARVPTT 64
>gb|BF628008.2|BF628008 HVSMEb0002J11f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0002J11f, mRNA sequence
Length = 665
Score = 147 bits (315), Expect(3) = 2e-047
Identities = 67/114 (58%), Positives = 81/114 (71%)
Frame = +1 / +3
Query: 58 RKDRPQLTLNFVVQFFFLALCGITANQGFYLLGLDNTSPTFASAIQNSVPAITFAMAAAL 237
+KDRP LTL+ + +FF LAL GITANQGFYLLGL + SPT+ASAIQN PAITF +AA L
Sbjct: 207 KKDRPPLTLSLLAEFFVLALIGITANQGFYLLGLYHLSPTYASAIQNMEPAITFVLAAEL 386
Query: 238 RIEKVRLDRRDGVAKVAGTLACVAXASVITLYKGPTIFXXXXXXXLELASTSKA 399
R+E+V L RR GVA V GT+ V A+VITLYKG +F L+ TS +
Sbjct: 387 RLEQVDLGRRHGVANVVGTVVSVGGATVITLYKGLPLFSHNLHVQAVLSWTSSS 548
Score = 53.3 bits (110), Expect(3) = 2e-047
Identities = 16/21 (76%), Positives = 19/21 (90%)
Frame = +1 / +3
Query: 415 NWTLGCVYLIGHCLSWSGWLV 477
NWTLGC ++GHCLSWSGW+V
Sbjct: 558 NWTLGCRLILGHCLSWSGWMV 620
Score = 26.7 bits (52), Expect(3) = 2e-047
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = +1 / +1
Query: 478 LQAPVLKKYPARL 516
LQ PVLK YPA+L
Sbjct: 622 LQVPVLKPYPAKL 660
>gb|BQ763476.1|BQ763476 EBro02_SQ008_B17_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ008_B17 5', mRNA sequence
Length = 536
Score = 162 bits (349), Expect(2) = 2e-041
Identities = 69/74 (93%), Positives = 71/74 (95%)
Frame = +1 / +3
Query: 58 RKDRPQLTLNFVVQFFFLALCGITANQGFYLLGLDNTSPTFASAIQNSVPAITFAMAAAL 237
+KDRP+LTL F VQFFFLALCGITANQGFYLLGLDNTSPTFASAIQNSVPAITF MAAAL
Sbjct: 273 KKDRPRLTLGFAVQFFFLALCGITANQGFYLLGLDNTSPTFASAIQNSVPAITFGMAAAL 452
Query: 238 RIEKVRLDRRDGVA 279
RIEKVRLDRRDGVA
Sbjct: 453 RIEKVRLDRRDGVA 494
Score = 111 bits (238), Expect(3) = 1e-028
Identities = 46/58 (79%), Positives = 48/58 (82%)
Frame = -3 / -2
Query: 224 MAKVMAGTEFWMADANVGDVLSRPRR*NPWFAVMPQSARKKNWTTKLRVSCGLSFLQQ 51
M KVMAGTEFWMA+A VGDVLSRPRR* PW AVMPQSARKKNWT K RVS G SF +
Sbjct: 439 MPKVMAGTEFWMAEAKVGDVLSRPRR*KPWLAVMPQSARKKNWTAKPRVSLGRSFFSR 266
Score = 27.2 bits (53), Expect(3) = 1e-028
Identities = 11/14 (78%), Positives = 11/14 (78%)
Frame = -3 / -3
Query: 320 TDAXATHASVPATL 279
TDA ATHA VP TL
Sbjct: 534 TDAPATHARVPTTL 493
Score = 26.3 bits (51), Expect(2) = 2e-041
Identities = 11/13 (84%), Positives = 11/13 (84%)
Frame = +1 / +2
Query: 283 VAGTLACVAXASV 321
V GTLACVA ASV
Sbjct: 497 VVGTLACVAGASV 535
Score = 24.9 bits (48), Expect(3) = 1e-028
Identities = 9/14 (64%), Positives = 12/14 (85%)
Frame = -2 / -1
Query: 276 YAVAAVQPDLLDPQ 235
+AVA V+P LLDP+
Sbjct: 491 HAVAPVEPHLLDPE 450
>gb|BF259970.1|BF259970 HVSMEf0020M03f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0020M03f, mRNA sequence
Length = 549
Score = 101 bits (216), Expect(2) = 4e-031
Identities = 47/96 (48%), Positives = 61/96 (63%)
Frame = +1 / +2
Query: 61 KDRPQLTLNFVVQFFFLALCGITANQGFYLLGLDNTSPTFASAIQNSVPAITFAMAAALR 240
K RP++T + F LAL G +Q FY GL TSPTF+ A+ N +PA+TF MA R
Sbjct: 23 KVRPRMTPLVFLNIFLLALMGPVIDQNFYYAGLKYTSPTFSCAMSNMLPAMTFVMAVIFR 202
Query: 241 IEKVRLDRRDGVAKVAGTLACVAXASVITLYKGPTI 348
+EKV L + +AKVAGTL VA A ++TLYKG +
Sbjct: 203 MEKVDLKKARCLAKVAGTLVTVAGAMLMTLYKGRAV 310
Score = 52.4 bits (108), Expect(2) = 4e-031
Identities = 20/52 (38%), Positives = 30/52 (57%)
Frame = +1 / +2
Query: 397 AVAGDKNWTLGCVYLIGHCLSWSGWLVLQAPVLKKYPARLAVTSYTCFFGVI 552
A A K+W G ++LI L+W+ +LQ P L +Y A L +T+ CF G +
Sbjct: 362 AAAAAKDWFKGSIFLIIATLAWASLFILQGPTLTRYNAPLTLTTLICFVGTL 517
>gb|BE060220.1|BE060220 HVSMEg0011K01f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0011K01f, mRNA sequence
Length = 645
Score = 121 bits (259), Expect = 3e-027
Identities = 45/47 (95%), Positives = 47/47 (100%)
Frame = +1 / +1
Query: 412 KNWTLGCVYLIGHCLSWSGWLVLQAPVLKKYPARLAVTSYTCFFGVI 552
KNWTLGCVYLIGHCLSWSGWLVLQAPVLKKYPA+L+VTSYTCFFGVI
Sbjct: 31 KNWTLGCVYLIGHCLSWSGWLVLQAPVLKKYPAKLSVTSYTCFFGVI 171
>gb|BI957153.1|BI957153 HVSMEn0007M22f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0007M22f, mRNA sequence
Length = 1133
Score = 121 bits (259), Expect = 3e-027
Identities = 45/47 (95%), Positives = 47/47 (100%)
Frame = +1 / +3
Query: 412 KNWTLGCVYLIGHCLSWSGWLVLQAPVLKKYPARLAVTSYTCFFGVI 552
KNWTLGCVYLIGHCLSWSGWLVLQAPVLKKYPA+L+VTSYTCFFGVI
Sbjct: 81 KNWTLGCVYLIGHCLSWSGWLVLQAPVLKKYPAKLSVTSYTCFFGVI 221
>gb|BF258425.1|BF258425 HVSMEf0015L01f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0015L01f, mRNA sequence
Length = 620
Score = 91.8 bits (194), Expect(2) = 2e-026
Identities = 44/96 (45%), Positives = 58/96 (60%)
Frame = +1 / +1
Query: 61 KDRPQLTLNFVVQFFFLALCGITANQGFYLLGLDNTSPTFASAIQNSVPAITFAMAAALR 240
K RP++T + F L L G +Q FY GL T PTF+ A+ N +PA+TF MA R
Sbjct: 25 KVRPRMTHLVFLNIFLLDLMGPVIHQNFYYAGLMYTYPTFSCAMSNMLPAMTFVMADIFR 204
Query: 241 IEKVRLDRRDGVAKVAGTLACVAXASVITLYKGPTI 348
+EKV L + +A VAGTL VA A ++TLYKG +
Sbjct: 205 MEKVDLTKAMCLANVAGTLVTVAGAMLMTLYKGRAV 312
Score = 46.9 bits (96), Expect(2) = 2e-026
Identities = 19/52 (36%), Positives = 28/52 (53%)
Frame = +1 / +1
Query: 397 AVAGDKNWTLGCVYLIGHCLSWSGWLVLQAPVLKKYPARLAVTSYTCFFGVI 552
A K+W G + LI L+W+ L+LQ P L +Y A L +T+ C G +
Sbjct: 364 AAVAAKDWFKGYILLIIRTLAWACLLILQGPTLTRYNAPLTLTTLICLVGTL 519
>gb|BU996125.1|BU996125 HM12I08r HM Hordeum vulgare subsp. vulgare cDNA clone HM12I08
5-PRIME, mRNA sequence
Length = 512
Score = 79.0 bits (166), Expect(2) = 2e-023
Identities = 34/67 (50%), Positives = 45/67 (67%)
Frame = +1 / +1
Query: 142 FYLLGLDNTSPTFASAIQNSVPAITFAMAAALRIEKVRLDRRDGVAKVAGTLACVAXASV 321
F L+GL T PTFA A+ N +PA+TF MA R+EK+ L + AK+ GT+ VA A +
Sbjct: 82 FLLVGLKYTGPTFACAMSNILPAMTFVMAVIFRMEKIELTKLRCQAKIFGTVVTVAGAML 261
Query: 322 ITLYKGP 342
+TLYKGP
Sbjct: 262 MTLYKGP 282
Score = 49.2 bits (101), Expect(2) = 2e-023
Identities = 17/47 (36%), Positives = 29/47 (61%)
Frame = +1 / +1
Query: 412 KNWTLGCVYLIGHCLSWSGWLVLQAPVLKKYPARLAVTSYTCFFGVI 552
+ W LG +++I L+W+ +LQ +KKY +L++T+ CF G I
Sbjct: 367 REWFLGSLFIIIATLAWASLFILQTHTIKKYTXQLSLTTLICFVGTI 507
>gb|CA021326.1|CA021326 HZ39M08r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ39M08
5-PRIME, mRNA sequence
Length = 567
Score = 73.5 bits (154), Expect(2) = 4e-023
Identities = 32/72 (44%), Positives = 46/72 (63%)
Frame = +1 / +1
Query: 133 NQGFYLLGLDNTSPTFASAIQNSVPAITFAMAAALRIEKVRLDRRDGVAKVAGTLACVAX 312
+Q Y +G TS +F+SA+ N +PA+TF MA LR+EK+RL AKVAGT+ V
Sbjct: 1 DQNLYYVGNKLTSASFSSALVNILPAVTFIMAIVLRMEKLRLRSSHSQAKVAGTICTVIG 180
Query: 313 ASVITLYKGPTI 348
A ++ +Y GP +
Sbjct: 181 AVLMIMYHGPVV 216
Score = 53.8 bits (111), Expect(2) = 4e-023
Identities = 19/58 (32%), Positives = 33/58 (56%)
Frame = +1 / +1
Query: 373 LELASTSKAVAGDKNWTLGCVYLIGHCLSWSGWLVLQAPVLKKYPARLAVTSYTCFFG 546
++ A+++ A W G + +I C++W+G+ VLQ+ L YPA L +T+ C G
Sbjct: 247 VDQAASAAAAQSSATWLKGTIAIITSCVAWAGFFVLQSNTLNSYPAALTLTTLICAMG 420
>gb|CA014589.1|CA014589 HT11M09r HT Hordeum vulgare subsp. vulgare cDNA clone HT11M09
5-PRIME, mRNA sequence
Length = 671
Score = 104 bits (221), Expect = 5e-022
Identities = 43/97 (44%), Positives = 62/97 (63%)
Frame = +1 / +1
Query: 58 RKDRPQLTLNFVVQFFFLALCGITANQGFYLLGLDNTSPTFASAIQNSVPAITFAMAAAL 237
R+ +T ++Q FF ++ G T NQ Y +GL +T+PT A A+ N++PA+TF MAA L
Sbjct: 304 RRSGMVITKQVLIQIFFSSIFGATLNQVLYFVGLKSTTPTVACALSNTLPALTFVMAAIL 483
Query: 238 RIEKVRLDRRDGVAKVAGTLACVAXASVITLYKGPTI 348
++E VRL G AKV GT+ CV + V+ YKGP +
Sbjct: 484 KMETVRLKTSSGKAKVLGTVVCVGGSMVMPFYKGPLL 594
>gb|BM443422.2|BM443422 EBro02_SQ005_B06_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ005_B06 5', mRNA sequence
Length = 243
Score = 100 bits (213), Expect = 6e-021
Identities = 38/40 (95%), Positives = 40/40 (100%)
Frame = +1 / +1
Query: 433 VYLIGHCLSWSGWLVLQAPVLKKYPARLAVTSYTCFFGVI 552
VYLIGHCLSWSGWLVLQAPVLKKYPA+L+VTSYTCFFGVI
Sbjct: 1 VYLIGHCLSWSGWLVLQAPVLKKYPAKLSVTSYTCFFGVI 120
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 203,835,921
Number of Sequences: 312970
Number of extensions: 2854409
Number of successful extensions: 66560
Number of sequences better than 1.0e-020: 19
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 5768506665
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)