TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3922840.2.1
(683 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU995861.1|BU995861 HM11J18r HM Hordeum vulgare subsp. v... 165 2e-040
gb|BU996696.1|BU996696 HM14H07r HM Hordeum vulgare subsp. v... 165 2e-040
gb|CB881055.1|CB881055 HM08K06w HM Hordeum vulgare subsp. v... 162 2e-039
gb|BM375547.2|BM375547 EBem06_SQ003_K19_R embryo, 21 DPA, n... 160 1e-038
>gb|BU995861.1|BU995861 HM11J18r HM Hordeum vulgare subsp. vulgare cDNA clone HM11J18
5-PRIME, mRNA sequence
Length = 598
Score = 165 bits (355), Expect = 2e-040
Identities = 56/73 (76%), Positives = 66/73 (90%)
Frame = -1 / +2
Query: 494 TTSPHGQAEAIDCGASCSYRCSKSGRPKMCLRACGTCCQRCGCVPPGTSGNEDVCPCYAN 315
TT+P GQA+A+DCG +CS+RCSKS RP +C RAC TCC+RCGCVPPGT+GNEDVCPCYA+
Sbjct: 122 TTAPRGQADAVDCGGACSFRCSKSSRPNLCGRACNTCCRRCGCVPPGTAGNEDVCPCYAH 301
Query: 314 MKTHDGQHKCP*S 276
M THDG+HKCP*S
Sbjct: 302 MTTHDGRHKCP*S 340
Score = 113 bits (241), Expect = 1e-024
Identities = 51/70 (72%), Positives = 51/70 (72%)
Frame = +1 / -2
Query: 277 DHGHLCWPSWVFMLA*QGQTSSLPEVPGGTQPQRWQQVPHALRHILGRPDLLHRYEQLAP 456
DHGHL PS V M A*QGQTSSLP VPGGTQPQR Q V HAL H GR DLLHR E P
Sbjct: 339 DHGHLWRPSCVVMWA*QGQTSSLPAVPGGTQPQRRQHVLHALPHRFGRLDLLHRNEHAPP 160
Query: 457 QSMASACPWG 486
QS ASACP G
Sbjct: 159 QSTASACPLG 130
>gb|BU996696.1|BU996696 HM14H07r HM Hordeum vulgare subsp. vulgare cDNA clone HM14H07
5-PRIME, mRNA sequence
Length = 467
Score = 165 bits (355), Expect = 2e-040
Identities = 56/73 (76%), Positives = 66/73 (90%)
Frame = -1 / +2
Query: 494 TTSPHGQAEAIDCGASCSYRCSKSGRPKMCLRACGTCCQRCGCVPPGTSGNEDVCPCYAN 315
TT+P GQA+A+DCG +CS+RCSKS RP +C RAC TCC+RCGCVPPGT+GNEDVCPCYA+
Sbjct: 74 TTAPRGQADAVDCGGACSFRCSKSSRPNLCGRACNTCCRRCGCVPPGTAGNEDVCPCYAH 253
Query: 314 MKTHDGQHKCP*S 276
M THDG+HKCP*S
Sbjct: 254 MTTHDGRHKCP*S 292
Score = 113 bits (241), Expect = 1e-024
Identities = 51/70 (72%), Positives = 51/70 (72%)
Frame = +1 / -3
Query: 277 DHGHLCWPSWVFMLA*QGQTSSLPEVPGGTQPQRWQQVPHALRHILGRPDLLHRYEQLAP 456
DHGHL PS V M A*QGQTSSLP VPGGTQPQR Q V HAL H GR DLLHR E P
Sbjct: 291 DHGHLWRPSCVVMWA*QGQTSSLPAVPGGTQPQRRQHVLHALPHRFGRLDLLHRNEHAPP 112
Query: 457 QSMASACPWG 486
QS ASACP G
Sbjct: 111 QSTASACPLG 82
>gb|CB881055.1|CB881055 HM08K06w HM Hordeum vulgare subsp. vulgare cDNA clone HM08K06
3-PRIME, mRNA sequence
Length = 512
Score = 162 bits (348), Expect = 2e-039
Identities = 55/73 (75%), Positives = 65/73 (89%)
Frame = -1 / -1
Query: 494 TTSPHGQAEAIDCGASCSYRCSKSGRPKMCLRACGTCCQRCGCVPPGTSGNEDVCPCYAN 315
TT+P GQA+A+DC +CS+RCSKS RP +C RAC TCC+RCGCVPPGT+GNEDVCPCYA+
Sbjct: 452 TTAPRGQADAVDCDGACSFRCSKSSRPNLCGRACNTCCRRCGCVPPGTAGNEDVCPCYAH 273
Query: 314 MKTHDGQHKCP*S 276
M THDG+HKCP*S
Sbjct: 272 MTTHDGRHKCP*S 234
Score = 109 bits (233), Expect = 1e-023
Identities = 50/70 (71%), Positives = 50/70 (71%)
Frame = +1 / +1
Query: 277 DHGHLCWPSWVFMLA*QGQTSSLPEVPGGTQPQRWQQVPHALRHILGRPDLLHRYEQLAP 456
DHGHL PS V M A*QGQTSSLP VPGGTQPQR Q V HAL H GR DLLHR E
Sbjct: 235 DHGHLWRPSCVVMWA*QGQTSSLPAVPGGTQPQRRQHVLHALPHRFGRLDLLHRNEHAPS 414
Query: 457 QSMASACPWG 486
QS ASACP G
Sbjct: 415 QSTASACPLG 444
>gb|BM375547.2|BM375547 EBem06_SQ003_K19_R embryo, 21 DPA, no treatment, cv Optic, EBem06
Hordeum vulgare subsp. vulgare cDNA clone
EBem06_SQ003_K19 5', mRNA sequence
Length = 501
Score = 160 bits (343), Expect = 1e-038
Identities = 55/73 (75%), Positives = 64/73 (87%)
Frame = -1 / +1
Query: 494 TTSPHGQAEAIDCGASCSYRCSKSGRPKMCLRACGTCCQRCGCVPPGTSGNEDVCPCYAN 315
TT+P GQA A+DCG +CSYRCSKS RP +C RAC TCC+RC CVPPGT+GNEDVCPCYA+
Sbjct: 133 TTAPIGQAHAVDCGGACSYRCSKSSRPNLCNRACNTCCRRCDCVPPGTAGNEDVCPCYAH 312
Query: 314 MKTHDGQHKCP*S 276
M TH+G+HKCP*S
Sbjct: 313 MTTHNGRHKCP*S 351
Score = 105 bits (225), Expect = 2e-022
Identities = 49/70 (70%), Positives = 49/70 (70%)
Frame = +1 / -2
Query: 277 DHGHLCWPSWVFMLA*QGQTSSLPEVPGGTQPQRWQQVPHALRHILGRPDLLHRYEQLAP 456
DHGHL P V M A*QGQTSSLP VPGGTQ QR Q V HAL H GR DLLHRYE P
Sbjct: 350 DHGHLWRPLCVVM*A*QGQTSSLPAVPGGTQSQRRQHVLHALLHRFGRLDLLHRYEHAPP 171
Query: 457 QSMASACPWG 486
QS A ACP G
Sbjct: 170 QSTA*ACPIG 141
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 295,111,900
Number of Sequences: 312970
Number of extensions: 4648957
Number of successful extensions: 70389
Number of sequences better than 1.0e-020: 5
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7465340784
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)