TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3871943.2.1
(1085 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI780849.2|BI780849 EBma05_SQ001_C24_R maternal, 12 DPA,... 319 1e-086
gb|BI950935.1|BI950935 HVSMEl0023L12f Hordeum vulgare spike... 313 1e-084
gb|BJ465418.1|BJ465418 BJ465418 K. Sato unpublished cDNA li... 172 2e-042
gb|BQ754059.1|BQ754059 EBca01_SQ002_L07_R carpel, pre-anthe... 159 3e-038
gb|BI956815.1|BI956815 HVSMEn0005H07f Hordeum vulgare rachi... 91 9e-035
gb|BI956859.1|BI956859 HVSMEn0005L01f Hordeum vulgare rachi... 91 2e-034
gb|CK122193.1|CK122193 BES1824101p15 BES1824 Hordeum vulgar... 101 1e-031
gb|CA029858.1|CA029858 HX05G15r HX Hordeum vulgare subsp. v... 81 9e-031
gb|BG415761.2|BG415761 HVSMEk0007L15f Hordeum vulgare testa... 133 2e-030
gb|CK122985.1|CK122985 BES1824101k13 BES1824 Hordeum vulgar... 101 3e-030
gb|BE422260.1|BE422260 HWM022cC.01r ITEC HWM Barley Leaf Li... 114 2e-029
gb|CB859122.1|CB859122 HI09G17w HI Hordeum vulgare subsp. v... 93 1e-027
gb|BQ765947.1|BQ765947 EBro03_SQ008_I17_R root, 3 week, wat... 105 2e-027
gb|CB859989.1|CB859989 HI12E03w HI Hordeum vulgare subsp. v... 93 7e-027
gb|BU990192.1|BU990192 HF24F16r HF Hordeum vulgare subsp. v... 105 2e-025
gb|BQ765469.1|BQ765469 EBro03_SQ007_C09_R root, 3 week, wat... 106 1e-024
gb|BJ468665.1|BJ468665 BJ468665 K. Sato unpublished cDNA li... 96 7e-024
gb|BM374246.2|BM374246 EBpi03_SQ003_B10_R pistil, 4 DPA, no... 94 3e-023
gb|BI954264.1|BI954264 HVSMEm0016O11f Hordeum vulgare green... 105 3e-022
>gb|BI780849.2|BI780849 EBma05_SQ001_C24_R maternal, 12 DPA, no treatment, cv Optic, EBma05
Hordeum vulgare subsp. vulgare cDNA clone
EBma05_SQ001_C24 5', mRNA sequence
Length = 602
Score = 319 bits (692), Expect = 1e-086
Identities = 125/199 (62%), Positives = 150/199 (75%)
Frame = -2 / +3
Query: 1078 QTVVTGDKSNXXXXXXXXXXXXXAEGNGFICKSMGFVNTAGPEGHQAVAMHVQGDKSVFY 899
Q+ VTG KS E GFICK+MGF NTAG E HQAVA+ VQGD S F+
Sbjct: 6 QSRVTGSKSFADGITTMKTATFSIEAAGFICKNMGFHNTAGAEKHQAVALRVQGDLSAFF 185
Query: 898 NCRFEGYQDTLYVHANRQFFRDCEVLGTVDFIFGNSAALFQNCLMTVRKPGDSQSNMVTA 719
NCRF+ +QDTLYVHA RQFFR+C + GT+DFIFGNSAA+FQNCL+ R+P D+Q N VTA
Sbjct: 186 NCRFDAFQDTLYVHARRQFFRNCVISGTIDFIFGNSAAVFQNCLIITRRPMDNQQNSVTA 365
Query: 718 QGRTDPNMPTGIVLQGCRIVPEQALFPDRLQIATYLGRPWKEYARTVVMESTIGDLIRPE 539
GRTDPNM +G+V+Q CR+VP+Q LF DR +I +YLGRPWKE++R V+MES I D I+PE
Sbjct: 366 HGRTDPNMKSGLVIQNCRLVPDQKLFVDRFKIPSYLGRPWKEFSRLVIMESQIADFIKPE 545
Query: 538 GWAEWMGDLGLKTLYYAEY 482
G+ W GD GLKTLYYAEY
Sbjct: 546 GYMPWNGDFGLKTLYYAEY 602
Score = 255 bits (552), Expect = 2e-067
Identities = 114/172 (66%), Positives = 124/172 (72%)
Frame = +3 / -2
Query: 483 YSA**SVLRPRSPIHSAHPSGLMRSPMVLSITTVLAYSFHGRPR*VAIWRRSGKSACSGT 662
YSA**SVL P+SP H +PSGLM+S + LS+ T SFHG PR* I +RS KS SGT
Sbjct: 601 YSA**SVLSPKSPFHGMYPSGLMKSAIWLSMMTSRENSFHGLPR*DGILKRSTKSFWSGT 422
Query: 663 MRQPWSTMPVGMLGSVRPCAVTMLDWLSPGLRTVMRQFWNSAAELPKMKSTVPSTSQSRK 842
RQ W T P MLGSVRPCAVT WLS G R +MRQFWN+AAELPKMKS VP +Q RK
Sbjct: 421 RRQFWMTSPDFMLGSVRPCAVTEFCWLSMGRRVMMRQFWNTAAELPKMKSMVPEMTQLRK 242
Query: 843 NCRLACTYSVSWYPSNRQL*NTDLSPCTCIATAWCPSGPAVFTNPIDLQMNP 998
NCR ACTYSVSW SNRQL D SPCT ATAWC S PAV NP+ LQMNP
Sbjct: 241 NCRRACTYSVSWNASNRQLKKADRSPCTLSATAWCFSAPAVLWNPMFLQMNP 86
Score = 150 bits (323), Expect = 1e-035
Identities = 74/123 (60%), Positives = 81/123 (65%)
Frame = +2 / -1
Query: 524 PLRPPFRPDEVADGALHHHRPRVLLPRPAEVGGDLEAVGEERLLRHDAAALEHDARGHVG 703
P P DEV D ALH +PR LLPRPA+VG D EAV EE L+RH AA L+ HVG
Sbjct: 560 PRHVPLGLDEVGDLALHDDQPRELLPRPAQVGRDFEAVHEELLVRHQAAVLDDQPGLHVG 381
Query: 704 VRPPLRRHHVGLAVPGLAHRHEAVLEQRRRVAEDEVHGAQHLAVAEELPVGVHVQRVLVP 883
VR P+R H V L V G EAVLE RRVAEDEV GA AVAEELP GVHVQRVL
Sbjct: 380 VRAPVRGHRVLLVVHGPPRDDEAVLEHGRRVAEDEVDGAGDDAVAEELPPGVHVQRVLER 201
Query: 884 LEP 892
++P
Sbjct: 200 VKP 192
>gb|BI950935.1|BI950935 HVSMEl0023L12f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0023L12f, mRNA sequence
Length = 536
Score = 313 bits (677), Expect = 1e-084
Identities = 117/176 (66%), Positives = 143/176 (81%)
Frame = -2 / +2
Query: 931 MHVQGDKSVFYNCRFEGYQDTLYVHANRQFFRDCEVLGTVDFIFGNSAALFQNCLMTVRK 752
+ VQGD S F+NCRF+ +QDTLYVHA RQFFR+C + GT+DFIFGNSAA+FQNCL+ R+
Sbjct: 2 LRVQGDLSAFFNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIFGNSAAVFQNCLIITRR 181
Query: 751 PGDSQSNMVTAQGRTDPNMPTGIVLQGCRIVPEQALFPDRLQIATYLGRPWKEYARTVVM 572
P D+Q N VTA GRTDPNM +G+V+Q CR+VP+Q LF DR +I +YLGRPWKE++R V+M
Sbjct: 182 PMDNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKLFVDRFKIPSYLGRPWKEFSRLVIM 361
Query: 571 ESTIGDLIRPEGWAEWMGDLGLKTLYYAEYANTGPGAGTSKRVNWPGYHVIGQADA 404
ES I D I+PEG+ W GD GLKTLYYAEY N GPGAGTSKRV WPG+ VI + +A
Sbjct: 362 ESQIADFIKPEGYMPWNGDFGLKTLYYAEYGNRGPGAGTSKRVTWPGFRVIARQEA 529
Score = 218 bits (471), Expect = 4e-056
Identities = 98/148 (66%), Positives = 107/148 (72%)
Frame = +3 / -3
Query: 483 YSA**SVLRPRSPIHSAHPSGLMRSPMVLSITTVLAYSFHGRPR*VAIWRRSGKSACSGT 662
YSA**SVL P+SP H +PSGLM+S + LS+ T SFHG PR* I +RS KS SGT
Sbjct: 450 YSA**SVLSPKSPFHGMYPSGLMKSAIWLSMMTSRENSFHGLPR*DGILKRSTKSFWSGT 271
Query: 663 MRQPWSTMPVGMLGSVRPCAVTMLDWLSPGLRTVMRQFWNSAAELPKMKSTVPSTSQSRK 842
RQ W T P MLGSVRPCAVT WLS G R +MRQFWN+AAELPKMKS VP +Q RK
Sbjct: 270 RRQFWMTSPDFMLGSVRPCAVTEFCWLSMGRRVMMRQFWNTAAELPKMKSMVPEMTQLRK 91
Query: 843 NCRLACTYSVSWYPSNRQL*NTDLSPCT 926
NCR ACTYSVSW SNRQL D SPCT
Sbjct: 90 NCRRACTYSVSWNASNRQLKKADRSPCT 7
Score = 150 bits (323), Expect(2) = 7e-038
Identities = 74/123 (60%), Positives = 81/123 (65%)
Frame = +2 / -2
Query: 524 PLRPPFRPDEVADGALHHHRPRVLLPRPAEVGGDLEAVGEERLLRHDAAALEHDARGHVG 703
P P DEV D ALH +PR LLPRPA+VG D EAV EE L+RH AA L+ HVG
Sbjct: 409 PRHVPLGLDEVGDLALHDDQPRELLPRPAQVGRDFEAVHEELLVRHQAAVLDDQPGLHVG 230
Query: 704 VRPPLRRHHVGLAVPGLAHRHEAVLEQRRRVAEDEVHGAQHLAVAEELPVGVHVQRVLVP 883
VR P+R H V L V G EAVLE RRVAEDEV GA AVAEELP GVHVQRVL
Sbjct: 229 VRAPVRGHRVLLVVHGPPRDDEAVLEHGRRVAEDEVDGAGDDAVAEELPPGVHVQRVLER 50
Query: 884 LEP 892
++P
Sbjct: 49 VKP 41
Score = 27.2 bits (53), Expect(2) = 7e-038
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = +1 / -1
Query: 871 CPGTPRTGSCRTPT 912
CPGT +TGS R PT
Sbjct: 62 CPGTRQTGS*RRPT 21
>gb|BJ465418.1|BJ465418 BJ465418 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags39m02 5', mRNA sequence
Length = 528
Score = 172 bits (371), Expect = 2e-042
Identities = 65/114 (57%), Positives = 84/114 (73%)
Frame = -2 / +1
Query: 1003 GNGFICKSMGFVNTAGPEGHQAVAMHVQGDKSVFYNCRFEGYQDTLYVHANRQFFRDCEV 824
GN F+ + + N AGP HQAVA+ V D S FY C F GYQDTLYVH+ RQFFR+C++
Sbjct: 181 GNNFLARDLTIENAAGPSKHQAVALRVGADLSAFYRCSFVGYQDTLYVHSLRQFFRECDI 360
Query: 823 LGTVDFIFGNSAALFQNCLMTVRKPGDSQSNMVTAQGRTDPNMPTGIVLQGCRI 662
GT+DF+FGNSAA+ Q+C + R+P +QSN+ TAQGRTDPN TGI +Q C++
Sbjct: 361 YGTIDFVFGNSAAVLQSCNLYARRPLPNQSNIYTAQGRTDPNQNTGISIQKCKV 522
Score = 116 bits (249), Expect = 2e-025
Identities = 55/99 (55%), Positives = 60/99 (60%)
Frame = +3 / -2
Query: 675 WSTMPVGMLGSVRPCAVTMLDWLSPGLRTVMRQFWNSAAELPKMKSTVPSTSQSRKNCRL 854
W MPV LGSVRPCAV ML WL G R Q W++AAELPK KS VP S SRKN R
Sbjct: 509 WMEMPVFWLGSVRPCAV*MLLWLGRGRRA*RLQLWSTAAELPKTKSMVP*MSHSRKN*RR 330
Query: 855 ACTYSVSWYPSNRQL*NTDLSPCTCIATAWCPSGPAVFT 971
CT VSW P+ * + S T ATAWC GPA F+
Sbjct: 329 ECT*RVSW*PTKLHR*KAERSAPTRSATAWCLDGPAAFS 213
>gb|BQ754059.1|BQ754059 EBca01_SQ002_L07_R carpel, pre-anthesis, no treatment, cv Optic,
EBca01 Hordeum vulgare subsp. vulgare cDNA clone
EBca01_SQ002_L07 5', mRNA sequence
Length = 372
Score = 159 bits (341), Expect = 3e-038
Identities = 63/108 (58%), Positives = 76/108 (70%)
Frame = -2 / +2
Query: 985 KSMGFVNTAGPEGHQAVAMHVQGDKSVFYNCRFEGYQDTLYVHANRQFFRDCEVLGTVDF 806
+ M F NTAGP HQAVA+ D SVFY C FEG+QDTLY H+ RQF+RDC V GTVDF
Sbjct: 5 RDMTFENTAGPAKHQAVALRCDSDLSVFYRCAFEGHQDTLYAHSLRQFYRDCRVAGTVDF 184
Query: 805 IFGNSAALFQNCLMTVRKPGDSQSNMVTAQGRTDPNMPTGIVLQGCRI 662
+FGN+AA+FQNCL+ R P Q N VTAQGR + +M +G Q C +
Sbjct: 185 VFGNAAAVFQNCLLLARAPLPGQKNSVTAQGRFNASMNSGFRFQFCNV 328
Score = 122 bits (260), Expect = 5e-027
Identities = 56/106 (52%), Positives = 62/106 (58%)
Frame = +3 / -1
Query: 669 QPWSTMPVGMLGSVRPCAVTMLDWLSPGLRTVMRQFWNSAAELPKMKSTVPSTSQSRKNC 848
Q W P M RP AVT W G R RQFWN+AA LPK KSTVP+T QSR N
Sbjct: 321 QNWKRKPEFMEALKRPWAVTEFFWPGSGARARRRQFWNTAAALPKTKSTVPATRQSR*NW 142
Query: 849 RLACTYSVSWYPSNRQL*NTDLSPCTCIATAWCPSGPAVFTNPIDL 986
R C YSVSW PS * T+ S ATAWC +GPAVF+N + L
Sbjct: 141 RSECAYSVSWCPSKAHR*KTERSESQRSATAWCLAGPAVFSNVMSL 4
>gb|BI956815.1|BI956815 HVSMEn0005H07f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0005H07f, mRNA sequence
Length = 877
Score = 91.3 bits (193), Expect(3) = 9e-035
Identities = 33/71 (46%), Positives = 50/71 (70%)
Frame = -2 / +3
Query: 856 ANRQFFRDCEVLGTVDFIFGNSAALFQNCLMTVRKPGDSQSNMVTAQGRTDPNMPTGIVL 677
+NRQF+R+C + GTVDFI+GN+ A+FQ+CL+ R P + N++TAQGR P+ +G
Sbjct: 6 SNRQFYRECVISGTVDFIYGNAKAVFQDCLLLARAPLKGKHNVITAQGRDGPDGSSGFSF 185
Query: 676 QGCRIVPEQAL 644
Q CR+ ++ L
Sbjct: 186 QNCRVTSQEDL 218
Score = 48.7 bits (100), Expect(3) = 9e-035
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = -2 / +3
Query: 619 TYLGRPWKEYARTVVMESTIGDLIRPEGWAEW 524
TY GRPWK ++ V M+S + ++ P GW W
Sbjct: 237 TYFGRPWKNHSHVVFMQSFLDTIVHPSGWVAW 332
Score = 45.5 bits (93), Expect(3) = 9e-035
Identities = 18/32 (56%), Positives = 21/32 (65%)
Frame = -2 / +3
Query: 502 TLYYAEYANTGPGAGTSKRVNWPGYHVIGQAD 407
T++Y EY NTGPGA SKRV W GQA+
Sbjct: 360 TVFYGEYGNTGPGANLSKRVPWLHVMDAGQAE 455
>gb|BI956859.1|BI956859 HVSMEn0005L01f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0005L01f, mRNA sequence
Length = 867
Score = 91.3 bits (193), Expect(3) = 2e-034
Identities = 33/71 (46%), Positives = 50/71 (70%)
Frame = -2 / +3
Query: 856 ANRQFFRDCEVLGTVDFIFGNSAALFQNCLMTVRKPGDSQSNMVTAQGRTDPNMPTGIVL 677
+NRQF+R+C + GTVDFI+GN+ A+FQ+CL+ R P + N++TAQGR P+ +G
Sbjct: 6 SNRQFYRECVISGTVDFIYGNAKAVFQDCLLLARAPLKGKHNVITAQGRDGPDGSSGFSF 185
Query: 676 QGCRIVPEQAL 644
Q CR+ ++ L
Sbjct: 186 QNCRVTSQEDL 218
Score = 48.7 bits (100), Expect(3) = 2e-034
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = -2 / +3
Query: 619 TYLGRPWKEYARTVVMESTIGDLIRPEGWAEW 524
TY GRPWK ++ V M+S + ++ P GW W
Sbjct: 237 TYFGRPWKNHSHVVFMQSFLDTIVHPSGWVAW 332
Score = 44.1 bits (90), Expect(3) = 2e-034
Identities = 17/32 (53%), Positives = 21/32 (65%)
Frame = -2 / +3
Query: 502 TLYYAEYANTGPGAGTSKRVNWPGYHVIGQAD 407
T++Y EY NTGPGA +KRV W GQA+
Sbjct: 360 TVFYGEYGNTGPGANLTKRVPWLHVMDAGQAE 455
>gb|CK122193.1|CK122193 BES1824101p15 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010P151 5-PRIME, mRNA sequence
Length = 641
Score = 101 bits (215), Expect(3) = 1e-031
Identities = 40/76 (52%), Positives = 51/76 (67%)
Frame = -2 / +1
Query: 1000 NGFICKSMGFVNTAGPEGHQAVAMHVQGDKSVFYNCRFEGYQDTLYVHANRQFFRDCEVL 821
+GF+ + + NTAGP+ HQAVA GD++V G+QDTLY HA RQF+ C V
Sbjct: 184 DGFMARDLTIANTAGPDAHQAVAFRSTGDRTVLDTVELAGHQDTLYAHAMRQFYTRCLVA 363
Query: 820 GTVDFIFGNSAALFQN 773
GTVDFIFGNSAA+ +
Sbjct: 364 GTVDFIFGNSAAVLHD 411
Score = 96.4 bits (204), Expect(3) = 7e-028
Identities = 41/74 (55%), Positives = 49/74 (66%)
Frame = +3 / -1
Query: 777 WNSAAELPKMKSTVPSTSQSRKNCRLACTYSVSWYPSNRQL*NTDLSPCTCIATAWCPSG 956
W++AAELPKMKSTVP+T NCR+AC YSVSW P++ + +T SP ATAWC SG
Sbjct: 407 WSTAAELPKMKSTVPATRHRV*NCRMACAYSVSWCPASSTVSSTVRSPVDRNATAWCASG 228
Query: 957 PAVFTNPIDLQMNP 998
PAVF M P
Sbjct: 227 PAVFAIVRSRAMKP 186
Score = 42.3 bits (86), Expect(3) = 1e-031
Identities = 16/28 (57%), Positives = 21/28 (75%)
Frame = -2 / +1
Query: 754 KPGDSQSNMVTAQGRTDPNMPTGIVLQG 671
+P +++ +TAQGRTDP PTGIVL G
Sbjct: 442 RPEKGETDALTAQGRTDPAQPTGIVLPG 525
Score = 36.8 bits (74), Expect(3) = 7e-028
Identities = 14/19 (73%), Positives = 16/19 (84%)
Frame = +3 / -1
Query: 672 PWSTMPVGMLGSVRPCAVT 728
P ST+PVG GSVRPCAV+
Sbjct: 524 PGSTIPVGCAGSVRPCAVS 468
Score = 31.3 bits (62), Expect(3) = 1e-031
Identities = 10/13 (76%), Positives = 12/13 (92%)
Frame = -2 / +2
Query: 616 YLGRPWKEYARTV 578
+LGRPWKEY+R V
Sbjct: 593 FLGRPWKEYSRPV 631
Score = 29.0 bits (57), Expect(3) = 7e-028
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = +2 / -2
Query: 578 HRPRVLLPRPAE 613
HR RVLLPRPAE
Sbjct: 631 HRSRVLLPRPAE 596
>gb|CA029858.1|CA029858 HX05G15r HX Hordeum vulgare subsp. vulgare cDNA clone HX05G15
5-PRIME, mRNA sequence
Length = 638
Score = 80.8 bits (170), Expect(4) = 9e-031
Identities = 28/63 (44%), Positives = 40/63 (63%)
Frame = -2 / +1
Query: 958 GPEGHQAVAMHVQGDKSVFYNCRFEGYQDTLYVHANRQFFRDCEVLGTVDFIFGNSAALF 779
G G VA+ + D + F C F G QDTLY H R +++DC + G+VDFIFGN+ +L+
Sbjct: 4 GRAGEAGVALRISADNAAFVGCNFLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLY 183
Query: 778 QNC 770
+ C
Sbjct: 184 EGC 192
Score = 61.6 bits (128), Expect(4) = 9e-031
Identities = 23/62 (37%), Positives = 35/62 (56%)
Frame = -2 / +1
Query: 622 ATYLGRPWKEYARTVVMESTIGDLIRPEGWAEWMGDLGLKTLYYAEYANTGPGAGTSKRV 443
A YLGR W ++R V + + ++I P GW W T++Y +Y +GPGA + RV
Sbjct: 304 ALYLGRAWGTFSRVVFAYTYMDNIIIPRGWYNWGDPTREMTVFYGQYKCSGPGANHAGRV 483
Query: 442 NW 437
+W
Sbjct: 484 DW 489
Score = 24.9 bits (48), Expect(4) = 9e-031
Identities = 11/21 (52%), Positives = 12/21 (57%)
Frame = -3 / +2
Query: 423 SSDRPTPRHSPPAPSSTARHG 361
SS P HS P+ SSTA G
Sbjct: 497 SSPTRRPSHSSPSASSTASSG 559
Score = 22.1 bits (42), Expect(4) = 9e-031
Identities = 11/32 (34%), Positives = 14/32 (43%)
Frame = -2 / +1
Query: 727 VTAQGRTDPNMPTGIVLQGCRIVPEQALFPDR 632
+TAQ R TG CR+ AL+ R
Sbjct: 226 LTAQNRRSMLDDTGFSFLNCRVTGSGALYLGR 321
>gb|BG415761.2|BG415761 HVSMEk0007L15f Hordeum vulgare testa/pericarp EST library HVcDNA0013
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEk0007L15f, mRNA sequence
Length = 650
Score = 133 bits (284), Expect = 2e-030
Identities = 54/126 (42%), Positives = 68/126 (53%)
Frame = -2 / +2
Query: 1075 TVVTGDKSNXXXXXXXXXXXXXAEGNGFICKSMGFVNTAGPEGHQAVAMHVQGDKSVFYN 896
TV+TG ++ +G I + NTA PE H AVA+ V D++V
Sbjct: 260 TVITGSRNVVDGATTFNSATLAVAADGVILXDLRIENTAVPEMHHAVALRVSADRAVINR 439
Query: 895 CRFEGYQDTLYVHANRQFFRDCEVLGTVDFIFGNSAALFQNCLMTVRKPGDSQSNMVTAQ 716
CR +GYQDTLY H R F+RDC V GTVDF F N+ + Q CL+T R+ Q VT
Sbjct: 440 CRVDGYQDTLYAHQLRDFYRDCAVCGTVDFFFSNAGVVLQGCLLTERRAARGQKKRVTGH 619
Query: 715 GRTDPN 698
G TDPN
Sbjct: 620 GMTDPN 637
>gb|CK122985.1|CK122985 BES1824101k13 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010K131 5-PRIME, mRNA sequence
Length = 611
Score = 101 bits (215), Expect(2) = 3e-030
Identities = 40/76 (52%), Positives = 51/76 (67%)
Frame = -2 / +1
Query: 1000 NGFICKSMGFVNTAGPEGHQAVAMHVQGDKSVFYNCRFEGYQDTLYVHANRQFFRDCEVL 821
+GF+ + + NTAGP+ HQAVA GD++V G+QDTLY HA RQF+ C V
Sbjct: 184 DGFMARDLTIANTAGPDAHQAVAFRSTGDRTVLDTVELAGHQDTLYAHAMRQFYTRCLVA 363
Query: 820 GTVDFIFGNSAALFQN 773
GTVDFIFGNSAA+ +
Sbjct: 364 GTVDFIFGNSAAVLHD 411
Score = 96.4 bits (204), Expect(2) = 9e-026
Identities = 41/74 (55%), Positives = 49/74 (66%)
Frame = +3 / -1
Query: 777 WNSAAELPKMKSTVPSTSQSRKNCRLACTYSVSWYPSNRQL*NTDLSPCTCIATAWCPSG 956
W++AAELPKMKSTVP+T NCR+AC YSVSW P++ + +T SP ATAWC SG
Sbjct: 407 WSTAAELPKMKSTVPATRHRV*NCRMACAYSVSWCPASSTVSSTVRSPVDRNATAWCASG 228
Query: 957 PAVFTNPIDLQMNP 998
PAVF M P
Sbjct: 227 PAVFAIVRSRAMKP 186
Score = 51.0 bits (105), Expect(2) = 3e-030
Identities = 18/31 (58%), Positives = 25/31 (80%)
Frame = -2 / +1
Query: 754 KPGDSQSNMVTAQGRTDPNMPTGIVLQGCRI 662
+P +++ +TAQGRTDP PTGIVL+GCR+
Sbjct: 442 RPEXGETDALTAQGRTDPAQPTGIVLRGCRV 534
Score = 40.9 bits (83), Expect(2) = 9e-026
Identities = 16/21 (76%), Positives = 18/21 (85%)
Frame = +3 / -1
Query: 666 RQPWSTMPVGMLGSVRPCAVT 728
RQP ST+PVG GSVRPCAV+
Sbjct: 530 RQPRSTIPVGCAGSVRPCAVS 468
>gb|BE422260.1|BE422260 HWM022cC.01r ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM022cC.01, mRNA sequence
Length = 1239
Score = 114 bits (244), Expect(2) = 2e-029
Identities = 42/70 (60%), Positives = 49/70 (70%)
Frame = -2 / +3
Query: 619 TYLGRPWKEYARTVVMESTIGDLIRPEGWAEWMGDLGLKTLYYAEYANTGPGAGTSKRVN 440
+YLGRPWK+Y+RTV M+S + ++ P GW W G L TLYY EY NTGPGAGTS RV
Sbjct: 117 SYLGRPWKQYSRTVFMQSELDSVVNPAGWLAWDGTFALDTLYYGEYQNTGPGAGTSGRVT 296
Query: 439 WPGYHVIGQA 410
W GY VI A
Sbjct: 297 WKGYRVITSA 326
Score = 35.0 bits (70), Expect(2) = 2e-029
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = -2 / +3
Query: 424 VIGQADATAFTAGAFIDGASWLQSTGTP 341
+ ++A+ FT G+FIDG WL T P
Sbjct: 315 ITSASEASTFTVGSFIDGDVWLAGTSIP 398
>gb|CB859122.1|CB859122 HI09G17w HI Hordeum vulgare subsp. vulgare cDNA clone HI09G17
3-PRIME, mRNA sequence
Length = 565
Score = 92.7 bits (196), Expect(2) = 1e-027
Identities = 31/56 (55%), Positives = 41/56 (73%)
Frame = -2 / -1
Query: 616 YLGRPWKEYARTVVMESTIGDLIRPEGWAEWMGDLGLKTLYYAEYANTGPGAGTSK 449
+LGRPWKEY+RTV + T+ ++++P GW W GD L TLYY EY + GPGAG S+
Sbjct: 376 FLGRPWKEYSRTVFVGCTLAEIVQPAGWMPWSGDFALDTLYYGEYGSAGPGAGRSR 209
Score = 51.0 bits (105), Expect(2) = 1e-027
Identities = 18/31 (58%), Positives = 25/31 (80%)
Frame = -2 / -1
Query: 754 KPGDSQSNMVTAQGRTDPNMPTGIVLQGCRI 662
+P +++ +TAQGRTDP PTGIVL+GCR+
Sbjct: 526 RPEKGETDALTAQGRTDPAQPTGIVLRGCRV 434
>gb|BQ765947.1|BQ765947 EBro03_SQ008_I17_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ008_I17 5', mRNA sequence
Length = 723
Score = 105 bits (225), Expect(2) = 2e-027
Identities = 42/87 (48%), Positives = 57/87 (65%)
Frame = -2 / +3
Query: 1006 EGNGFICKSMGFVNTAGPEGHQAVAMHVQGDKSVFYNCRFEGYQDTLYVHANRQFFRDCE 827
EG FI +++ F N+A QAVA+ V DK FY+CRF G+QDTLY+H +Q+ RDC
Sbjct: 12 EGEDFIAENITFQNSAPQGSGQAVAVRVTADKCAFYSCRFLGWQDTLYLHYGKQYLRDCY 191
Query: 826 VLGTVDFIFGNSAALFQNCLMTVRKPG 746
+ G DFIFGNS AL ++C + + G
Sbjct: 192 IEGNCDFIFGNSIALLEHCHIHCKSAG 272
Score = 39.6 bits (80), Expect(2) = 2e-027
Identities = 16/50 (32%), Positives = 24/50 (48%)
Frame = -2 / +1
Query: 586 RTVVMESTIGDLIRPEGWAEWMGDLGLKTLYYAEYANTGPGAGTSKRVNW 437
R V + + ++ GW W +T + EY +GPG+ TS RV W
Sbjct: 400 RVVFAHTFMDRCVKATGWHNWDKSENERTACFYEYRCSGPGSRTSSRVAW 549
>gb|CB859989.1|CB859989 HI12E03w HI Hordeum vulgare subsp. vulgare cDNA clone HI12E03
3-PRIME, mRNA sequence
Length = 521
Score = 92.7 bits (196), Expect(2) = 7e-027
Identities = 31/56 (55%), Positives = 41/56 (73%)
Frame = -2 / -1
Query: 616 YLGRPWKEYARTVVMESTIGDLIRPEGWAEWMGDLGLKTLYYAEYANTGPGAGTSK 449
+LGRPWKEY+RTV + T+ ++++P GW W GD L TLYY EY + GPGAG S+
Sbjct: 377 FLGRPWKEYSRTVFVGCTLAEIVQPAGWMPWSGDFALDTLYYGEYGSAGPGAGRSR 210
Score = 48.3 bits (99), Expect(2) = 7e-027
Identities = 17/26 (65%), Positives = 23/26 (88%)
Frame = -2 / -1
Query: 739 QSNMVTAQGRTDPNMPTGIVLQGCRI 662
+++ +TAQGRTDP PTGIVL+GCR+
Sbjct: 512 ETDALTAQGRTDPAQPTGIVLRGCRV 435
>gb|BU990192.1|BU990192 HF24F16r HF Hordeum vulgare subsp. vulgare cDNA clone HF24F16
5-PRIME, mRNA sequence
Length = 463
Score = 105 bits (223), Expect(2) = 2e-025
Identities = 39/60 (65%), Positives = 42/60 (70%)
Frame = -2 / +2
Query: 616 YLGRPWKEYARTVVMESTIGDLIRPEGWAEWMGDLGLKTLYYAEYANTGPGAGTSKRVNW 437
YLGRPWK Y+RTVVM S++ I P GW EW G L TLYY EY NTGPGAGT RV W
Sbjct: 5 YLGRPWKAYSRTVVMGSSLDRWIAPAGWLEWSGQFALSTLYYGEYGNTGPGAGTGGRVKW 184
Score = 31.3 bits (62), Expect(2) = 2e-025
Identities = 14/31 (45%), Positives = 15/31 (48%)
Frame = -2 / +2
Query: 421 IGQADATAFTAGAFIDGASWLQSTGTPNVMG 329
+ DAT FT FI G SWL TG G
Sbjct: 194 LSTVDATRFTVRDFILGDSWLGDTGVSYTSG 286
>gb|BQ765469.1|BQ765469 EBro03_SQ007_C09_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ007_C09 5', mRNA sequence
Length = 417
Score = 106 bits (227), Expect(2) = 1e-024
Identities = 36/61 (59%), Positives = 46/61 (75%)
Frame = -2 / +2
Query: 601 WKEYARTVVMESTIGDLIRPEGWAEWMGDLGLKTLYYAEYANTGPGAGTSKRVNWPGYHV 422
WK Y+RTV+M+S + ++ P GW W GD L TLYYAEY N+G G+ TS+RVNWPGYHV
Sbjct: 2 WKMYSRTVIMQSDVAGIVDPAGWMPWSGDFALSTLYYAEYDNSGAGSDTSRRVNWPGYHV 181
Query: 421 I 419
+
Sbjct: 182 L 184
Score = 26.7 bits (52), Expect(2) = 1e-024
Identities = 11/27 (40%), Positives = 13/27 (48%)
Frame = -2 / +2
Query: 409 DATAFTAGAFIDGASWLQSTGTPNVMG 329
DA FT + G WL TG P +G
Sbjct: 197 DAGNFTVANMVLGDFWLPQTGVPFTVG 277
>gb|BJ468665.1|BJ468665 BJ468665 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags39m02 3', mRNA sequence
Length = 606
Score = 95.9 bits (203), Expect(2) = 7e-024
Identities = 36/63 (57%), Positives = 42/63 (66%)
Frame = -2 / -3
Query: 598 KEYARTVVMESTIGDLIRPEGWAEWMGDLGLKTLYYAEYANTGPGAGTSKRVNWPGYHVI 419
K+Y+RTV M+S + ++ P GW W G L TLYY EY NTGPGAGTS RV W GY VI
Sbjct: 604 KQYSRTVFMQSELDSVVNPAGWLAWDGTFALDTLYYGEYQNTGPGAGTSGRVTWKGYRVI 425
Query: 418 GQA 410
A
Sbjct: 424 TSA 416
Score = 35.0 bits (70), Expect(2) = 7e-024
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = -2 / -3
Query: 424 VIGQADATAFTAGAFIDGASWLQSTGTP 341
+ ++A+ FT G+FIDG WL T P
Sbjct: 427 ITSASEASTFTVGSFIDGDVWLAGTSIP 344
>gb|BM374246.2|BM374246 EBpi03_SQ003_B10_R pistil, 4 DPA, no treatment, cv Optic, EBpi03
Hordeum vulgare subsp. vulgare cDNA clone
EBpi03_SQ003_B10 5', mRNA sequence
Length = 449
Score = 93.6 bits (198), Expect(2) = 3e-023
Identities = 35/62 (56%), Positives = 41/62 (66%)
Frame = -2 / +2
Query: 595 EYARTVVMESTIGDLIRPEGWAEWMGDLGLKTLYYAEYANTGPGAGTSKRVNWPGYHVIG 416
+Y+RTV M+S + ++ P GW W G L TLYY EY NTGPGAGTS RV W GY VI
Sbjct: 2 QYSRTVFMQSELDSVVNPAGWLAWDGTFALDTLYYGEYQNTGPGAGTSGRVTWKGYRVIT 181
Query: 415 QA 410
A
Sbjct: 182 SA 187
Score = 35.0 bits (70), Expect(2) = 3e-023
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = -2 / +2
Query: 424 VIGQADATAFTAGAFIDGASWLQSTGTP 341
+ ++A+ FT G+FIDG WL T P
Sbjct: 176 ITSASEASTFTVGSFIDGDVWLAGTSIP 259
>gb|BI954264.1|BI954264 HVSMEm0016O11f Hordeum vulgare green seedling EST library HVcDNA0014
(Blumeria infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEm0016O11f, mRNA sequence
Length = 674
Score = 105 bits (225), Expect = 3e-022
Identities = 42/87 (48%), Positives = 57/87 (65%)
Frame = -2 / +2
Query: 1006 EGNGFICKSMGFVNTAGPEGHQAVAMHVQGDKSVFYNCRFEGYQDTLYVHANRQFFRDCE 827
EG FI +++ F N+A QAVA+ V DK FY+CRF G+QDTLY+H +Q+ RDC
Sbjct: 227 EGEDFIAENITFQNSAPQGSGQAVAVRVTADKCAFYSCRFLGWQDTLYLHYGKQYLRDCY 406
Query: 826 VLGTVDFIFGNSAALFQNCLMTVRKPG 746
+ G DFIFGNS AL ++C + + G
Sbjct: 407 IEGNCDFIFGNSIALLEHCHIHCKSAG 487
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 457,990,914
Number of Sequences: 312970
Number of extensions: 7966440
Number of successful extensions: 124726
Number of sequences better than 1.0e-020: 25
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 12871556852
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)