TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3802264.2.1
(1189 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CK125884.1|CK125884 BES1824111A2a04 BES1824 Hordeum vulg... 67 7e-037
gb|BF259829.2|BF259829 HVSMEf0020G01f Hordeum vulgare seedl... 123 1e-036
gb|BF256953.2|BF256953 HVSMEf0011H11f Hordeum vulgare seedl... 125 2e-035
gb|BG369061.1|BG369061 HVSMEi0021J08f Hordeum vulgare 20 DA... 74 9e-032
gb|BU995458.1|BU995458 HM10F02r HM Hordeum vulgare subsp. v... 74 8e-031
gb|CA023829.1|CA023829 HZ47I13r HZ Hordeum vulgare subsp. v... 133 1e-030
gb|BU985192.1|BU985192 HF06G12r HF Hordeum vulgare subsp. v... 90 2e-029
gb|BQ766009.1|BQ766009 EBro03_SQ008_D24_R root, 3 week, wat... 65 3e-027
gb|BI960651.1|BI960651 HVSMEn0025K02f Hordeum vulgare rachi... 63 7e-027
dbj|AB040769.1| Hordeum vulgare mRNA for endo-1,4-beta-gluc... 67 7e-027
gb|CK123410.1|CK123410 BES1824103p13 BES1824 Hordeum vulgar... 75 2e-026
gb|AW982232.3|AW982232 HVSMEg0002G13f Hordeum vulgare pre-a... 67 4e-026
gb|BG418595.1|BG418595 HVSMEk0023G06f Hordeum vulgare testa... 67 4e-026
gb|BE601584.3|BE601584 HVSMEh0098M02f Hordeum vulgare 5-45 ... 67 4e-026
gb|BM097444.2|BM097444 EBro05_SQ003_F03_R root, 3 week, red... 67 4e-026
gb|AW983004.3|AW983004 HVSMEg0004M14f Hordeum vulgare pre-a... 67 1e-024
gb|CK122715.1|CK122715 BES1824105i22 BES1824 Hordeum vulgar... 67 2e-024
gb|CK123273.1|CK123273 BES1824103c09 BES1824 Hordeum vulgar... 67 2e-024
gb|BQ755502.1|BQ755502 EBed07_SQ002_E24_R endosperm, 28 DPA... 74 4e-024
gb|AV927273.1|AV927273 AV927273 K. Sato unpublished cDNA li... 67 8e-024
gb|BM099274.2|BM099274 EBes01_SQ002_M04_R embryo sac, 4-6 D... 109 3e-023
gb|AV916435.1|AV916435 AV916435 K. Sato unpublished cDNA li... 67 5e-023
gb|BQ460303.1|BQ460303 HA06K13r HA Hordeum vulgare subsp. v... 67 7e-023
gb|CK125396.1|CK125396 BES1824107o06 BES1824 Hordeum vulgar... 66 1e-022
gb|BQ760319.1|BQ760319 EBpi07_SQ003_L24_R pistil, 12 DPA, n... 63 3e-022
gb|BJ467008.1|BJ467008 BJ467008 K. Sato unpublished cDNA li... 65 5e-022
gb|BU985662.1|BU985662 HF08E02r HF Hordeum vulgare subsp. v... 67 6e-022
gb|CB875548.1|CB875548 HX08K06w HX Hordeum vulgare subsp. v... 63 9e-022
>gb|CK125884.1|CK125884 BES1824111A2a04 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010A0411 5-PRIME, mRNA sequence
Length = 748
Score = 67.0 bits (140), Expect(3) = 7e-037
Identities = 30/63 (47%), Positives = 36/63 (57%)
Frame = +1 / +2
Query: 718 VNCGLSFGDWMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLA 897
V+C + W NPN L GAIVGGPD+ D F D+R N TE TY N+ +G LA
Sbjct: 380 VSCRGGYATWYPRKGSNPNLLDGAIVGGPDEYDNFADERNNYEQTEATTYNNAPFMGVLA 559
Query: 898 ALA 906
LA
Sbjct: 560 RLA 568
Score = 66.1 bits (138), Expect(3) = 7e-037
Identities = 26/52 (50%), Positives = 35/52 (67%)
Frame = +1 / +2
Query: 535 VLCGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGAS 690
V C PA + FA+ Q+DY+LG+NPR +SY+VG+G P + HHRGAS
Sbjct: 194 VRCPAGAASPAEIMAFAKSQVDYILGSNPRGTSYMVGYGFIYPLEAHHRGAS 349
Score = 59.7 bits (124), Expect(3) = 7e-037
Identities = 27/61 (44%), Positives = 36/61 (59%)
Frame = +1 / +2
Query: 349 QNFKSQADNFVCAVLPDTAFHQVFITPGGVIHLRDGANSQYVTSTAFLLVVYADLLLRTG 528
Q ++ +AD FVCA L V TPGG++H + N Q+VTS +FLL Y+D L G
Sbjct: 5 QRYRQKADFFVCASLGKQGSGNVKRTPGGLLHHQKWNNLQFVTSGSFLLAAYSDSLAAAG 184
Query: 529 Q 531
Q
Sbjct: 185 Q 187
>gb|BF259829.2|BF259829 HVSMEf0020G01f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0020G01f, mRNA sequence
Length = 534
Score = 123 bits (264), Expect(2) = 1e-036
Identities = 51/116 (43%), Positives = 73/116 (62%)
Frame = +1 / +3
Query: 349 QNFKSQADNFVCAVLPDTAFHQVFITPGGVIHLRDGANSQYVTSTAFLLVVYADLLLRTG 528
+ F+++A+ F+CA + + V TPGG++++ D N QYV+S+ FLL VYAD L +G
Sbjct: 12 KQFRAKAEFFMCACIQKNGGNNVKTTPGGLLYVADWNNMQYVSSSVFLLTVYADYLAESG 191
Query: 529 QTVLCGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGASTP 696
+ C + + PA + FAR Q+DY+LG NP SY+VG PT HHRGAS P
Sbjct: 192 DKLKCPDGEVAPAEIVAFARSQVDYVLGKNPLSMSYMVGHSDKFPTHVHHRGASIP 359
Score = 50.1 bits (103), Expect(2) = 1e-036
Identities = 22/47 (46%), Positives = 26/47 (55%)
Frame = +1 / +3
Query: 718 VNCGLSFGDWMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEP 858
V C F + +PN L GA+VGGPD +DGFVD R N EP
Sbjct: 384 VGCMEGFDAYYNSKGADPNVLVGALVGGPDGHDGFVDDRCNYQRAEP 524
>gb|BF256953.2|BF256953 HVSMEf0011H11f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0011H11f, mRNA sequence
Length = 819
Score = 125 bits (267), Expect(2) = 2e-035
Identities = 51/116 (43%), Positives = 74/116 (63%)
Frame = +1 / +3
Query: 349 QNFKSQADNFVCAVLPDTAFHQVFITPGGVIHLRDGANSQYVTSTAFLLVVYADLLLRTG 528
+ F+++A+ F+CA + + + V TPGG++++ D N QYV+S+ FLL VYAD L +G
Sbjct: 12 KQFRAKAEFFMCACIQENGGNNVKTTPGGLLYVADWNNMQYVSSSVFLLTVYADYLAESG 191
Query: 529 QTVLCGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGASTP 696
+ C + + PA + FAR Q+DY+LG NP SY+VG PT HHRGAS P
Sbjct: 192 DKLKCPDGEVAPAEIVAFARSQVDYVLGKNPLSMSYMVGHSDKFPTHVHHRGASIP 359
Score = 44.6 bits (91), Expect(2) = 2e-035
Identities = 22/63 (34%), Positives = 29/63 (46%)
Frame = +1 / +3
Query: 718 VNCGLSFGDWMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLA 897
V C F + +PN L A+VGGPD++DGF D R + EP + G
Sbjct: 384 VGCMEGFDAYYNSKGADPNVLVSALVGGPDRHDGFGDDRCSYQRAEPTLAAGAPMCGVFG 563
Query: 898 ALA 906
LA
Sbjct: 564 RLA 572
>gb|BG369061.1|BG369061 HVSMEi0021J08f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0021J08f, mRNA sequence
Length = 797
Score = 74.4 bits (156), Expect(3) = 9e-032
Identities = 31/62 (50%), Positives = 36/62 (58%)
Frame = +1 / +3
Query: 718 VNCGLSFGDWMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLA 897
+ C F DW A +PNPN LTGAIVGGP+ D F D RGN TE CTY + + A
Sbjct: 363 IGCMQGFDDWFARKRPNPNVLTGAIVGGPNCRDEFRDDRGNYVQTEACTYNTAPMVAVFA 542
Query: 898 AL 903
L
Sbjct: 543 RL 548
Score = 57.4 bits (119), Expect(3) = 9e-032
Identities = 22/45 (48%), Positives = 28/45 (62%)
Frame = +1 / +3
Query: 556 LPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGAS 690
+PP+ L AR Q +Y+LG NP SY+VG+G P HHR AS
Sbjct: 198 VPPSELLALARSQAEYILGRNPLRLSYMVGYGPRYPAHVHHRAAS 332
Score = 43.7 bits (89), Expect(3) = 9e-032
Identities = 20/52 (38%), Positives = 30/52 (57%)
Frame = +1 / +3
Query: 373 NFVCAVLPDTAFHQVFITPGGVIHLRDGANSQYVTSTAFLLVVYADLLLRTG 528
+++CA L V +PGG++++R N QY +S AFLL VY+ L G
Sbjct: 6 HYLCACLRLNNGSNVDRSPGGMLYVRQWNNLQYASSAAFLLTVYSRYLAGAG 161
>gb|BU995458.1|BU995458 HM10F02r HM Hordeum vulgare subsp. vulgare cDNA clone HM10F02
5-PRIME, mRNA sequence
Length = 628
Score = 74.4 bits (156), Expect(3) = 8e-031
Identities = 31/62 (50%), Positives = 36/62 (58%)
Frame = +1 / +1
Query: 718 VNCGLSFGDWMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLA 897
+ C F DW A +PNPN LTGAIVGGP+ D F D RGN TE CTY + + A
Sbjct: 367 IGCMQGFDDWFARKRPNPNVLTGAIVGGPNCRDEFRDDRGNYVQTEACTYNTAPMVAVFA 546
Query: 898 AL 903
L
Sbjct: 547 RL 552
Score = 57.4 bits (119), Expect(3) = 8e-031
Identities = 22/45 (48%), Positives = 28/45 (62%)
Frame = +1 / +1
Query: 556 LPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGAS 690
+PP+ L AR Q +Y+LG NP SY+VG+G P HHR AS
Sbjct: 202 VPPSELLALARSQAEYILGRNPLRLSYMVGYGPRYPAHVHHRAAS 336
Score = 40.5 bits (82), Expect(3) = 8e-031
Identities = 16/35 (45%), Positives = 23/35 (65%)
Frame = +1 / +1
Query: 424 TPGGVIHLRDGANSQYVTSTAFLLVVYADLLLRTG 528
+PGG++++R N QY +S AFLL VY+ L G
Sbjct: 61 SPGGMLYVRQWNNLQYASSAAFLLTVYSRYLAGAG 165
>gb|CA023829.1|CA023829 HZ47I13r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ47I13
5-PRIME, mRNA sequence
Length = 460
Score = 133 bits (286), Expect = 1e-030
Identities = 54/119 (45%), Positives = 77/119 (64%)
Frame = +1 / +2
Query: 340 GGAQNFKSQADNFVCAVLPDTAFHQVFITPGGVIHLRDGANSQYVTSTAFLLVVYADLLL 519
G Q +K+ AD+F+C+++P T Q T GG++ +N QYVTS++FLL+ YA L+
Sbjct: 14 GALQEYKAHADSFICSMVPGTPTDQTTYTRGGLLFKLSDSNMQYVTSSSFLLLTYAKYLV 193
Query: 520 RTGQTVLCGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGASTP 696
+TV CG + P RL AR+Q+DYLLG+NP SY+VG+GA P + HHR +S P
Sbjct: 194 SAKKTVSCGGVAVTPQRLRAIARRQVDYLLGSNPMGMSYMVGYGAKYPKKLHHRASSLP 370
>gb|BU985192.1|BU985192 HF06G12r HF Hordeum vulgare subsp. vulgare cDNA clone HF06G12
5-PRIME, mRNA sequence
Length = 615
Score = 90.0 bits (190), Expect(2) = 2e-029
Identities = 38/79 (48%), Positives = 52/79 (65%)
Frame = +1 / +3
Query: 460 NSQYVTSTAFLLVVYADLLLRTGQTVLCGNQPLPPARLHEFARQQMDYLLGANPRHSSYV 639
N Q+VTS +FLL+ YA L ++ TV CG+ + P L A++Q+DYLLG NP SY+
Sbjct: 3 NMQHVTSISFLLLTYAKYLTKSSHTVNCGDISVGPDTLRLQAKKQIDYLLGDNPMKMSYM 182
Query: 640 VGFGANPPTQPHHRGASTP 696
VG+G P + HHRG+S P
Sbjct: 183 VGYGDRYPQRIHHRGSSLP 239
Score = 60.2 bits (125), Expect(2) = 2e-029
Identities = 24/45 (53%), Positives = 31/45 (68%)
Frame = +1 / +3
Query: 763 PNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLA 897
PNPN L GAIVGGP ++D + D R + +EP TYIN+ +G LA
Sbjct: 306 PNPNPLVGAIVGGPGEDDSYGDDRADYRKSEPTTYINAPLVGVLA 440
>gb|BQ766009.1|BQ766009 EBro03_SQ008_D24_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ008_D24 5', mRNA sequence
Length = 613
Score = 64.8 bits (135), Expect(3) = 3e-027
Identities = 28/70 (40%), Positives = 31/70 (44%)
Frame = +1 / +2
Query: 658 PPTQPHHRGASTPVLPPGTDVNCGLSFGDWMAPDKPNPNELTGAIVGGPDKNDGFVDKRG 837
P H + P + C F W NPN L GAIVGGPD+ D F D R
Sbjct: 386 PARVHHRAASIVPYKHSKQFIGCTQGFDHWFGRRSSNPNVLVGAIVGGPDRRDRFRDNRD 565
Query: 838 NSSYTEPCTY 867
N TE CTY
Sbjct: 566 NYMQTEACTY 595
Score = 49.6 bits (102), Expect(3) = 3e-027
Identities = 17/26 (65%), Positives = 19/26 (73%)
Frame = +2 / +3
Query: 587 GSRWTTCWAPTRGTAPTSWASAPTRP 664
G RWTTCWA TRG TSW +AP+ P
Sbjct: 312 GRRWTTCWAATRGG*ATSWGTAPSTP 389
Score = 46.0 bits (94), Expect(3) = 3e-027
Identities = 18/35 (51%), Positives = 24/35 (68%)
Frame = +1 / +2
Query: 424 TPGGVIHLRDGANSQYVTSTAFLLVVYADLLLRTG 528
+PGG++++R N QYVT+ AFLL YAD L G
Sbjct: 143 SPGGMLYIRQWNNMQYVTNAAFLLSTYADYLAGAG 247
>gb|BI960651.1|BI960651 HVSMEn0025K02f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0025K02f, mRNA sequence
Length = 632
Score = 63.4 bits (132), Expect(3) = 7e-027
Identities = 27/69 (39%), Positives = 31/69 (44%)
Frame = +1 / +3
Query: 658 PPTQPHHRGASTPVLPPGTDVNCGLSFGDWMAPDKPNPNELTGAIVGGPDKNDGFVDKRG 837
P H + P + C F W NPN L GAIVGGPD+ D F D R
Sbjct: 312 PARVHHRAASIVPYKHSKQFIGCTQGFXHWFXRXSSNPNVLVGAIVGGPDREDRFRDNRD 491
Query: 838 NSSYTEPCT 864
N +TE CT
Sbjct: 492 NYMHTEACT 518
Score = 49.6 bits (102), Expect(3) = 7e-027
Identities = 17/26 (65%), Positives = 19/26 (73%)
Frame = +2 / +1
Query: 587 GSRWTTCWAPTRGTAPTSWASAPTRP 664
G RWTTCWA TRG TSW +AP+ P
Sbjct: 238 GRRWTTCWAATRGG*ATSWGTAPSTP 315
Score = 46.0 bits (94), Expect(3) = 7e-027
Identities = 18/35 (51%), Positives = 24/35 (68%)
Frame = +1 / +3
Query: 424 TPGGVIHLRDGANSQYVTSTAFLLVVYADLLLRTG 528
+PGG++++R N QYVT+ AFLL YAD L G
Sbjct: 69 SPGGMLYIRQWNNMQYVTNAAFLLSTYADYLAGAG 173
>dbj|AB040769.1| Hordeum vulgare mRNA for endo-1,4-beta-glucanase Cel1, complete cds
Length = 2442
Score = 67.0 bits (140), Expect(4) = 7e-027
Identities = 27/52 (51%), Positives = 33/52 (63%)
Frame = +1 / +1
Query: 541 CGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGASTP 696
CG L +FA+ Q+DY+LG NP+ SYVVGFG P + HHRGAS P
Sbjct: 1591 CGPNFYTTDVLRKFAKSQLDYILGKNPQKMSYVVGFGKKYPKRVHHRGASIP 1746
Score = 63.8 bits (133), Expect(4) = 7e-027
Identities = 27/54 (50%), Positives = 33/54 (61%)
Frame = +1 / +1
Query: 745 WMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLAALA 906
W K NPN L GA+V GPDK+DGF D R N +YTEP N+ + L +LA
Sbjct: 1786 WRESKKANPNILVGAMVAGPDKHDGFKDIRTNYNYTEPTLAANAGLVAALISLA 1947
Score = 30.9 bits (61), Expect(4) = 7e-027
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = +1 / +1
Query: 256 SSSVAEFSWDLKFPGAQVLLAEFNMTSAGG 345
S + F+WD K PG+QVLL+ + + G
Sbjct: 1285 SPNYGVFTWDDKLPGSQVLLSRLRLFLSPG 1374
Score = 25.4 bits (49), Expect(4) = 7e-027
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +1 / +1
Query: 466 QYVTSTAFLLVVYADLL 516
QYV + AFL +YAD L
Sbjct: 1513 QYVVNAAFLASLYADYL 1563
>gb|CK123410.1|CK123410 BES1824103p13 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010P133 5-PRIME, mRNA sequence
Length = 579
Score = 74.8 bits (157), Expect(2) = 2e-026
Identities = 30/61 (49%), Positives = 40/61 (65%)
Frame = +1 / +1
Query: 514 LLRTGQTVLCGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGAST 693
L+ +TV CG + P RL AR+Q+DYLLG+NP SY+VG+GA P + HHR +S
Sbjct: 1 LVSAKKTVSCGGVAVTPQRLRAIARRQVDYLLGSNPMGMSYMVGYGAKYPKKLHHRASSL 180
Query: 694 P 696
P
Sbjct: 181 P 183
Score = 64.8 bits (135), Expect(2) = 2e-026
Identities = 27/53 (50%), Positives = 36/53 (67%)
Frame = +1 / +1
Query: 766 NPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLAALAVRGVQL 924
NPN GA+VGGP++ND F D+R + ++EP TYIN+ +G LA LA QL
Sbjct: 253 NPNVHVGAVVGGPNQNDQFPDQRSDYEHSEPATYINAPLVGALAYLAHSSGQL 411
>gb|AW982232.3|AW982232 HVSMEg0002G13f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0002G13f, mRNA sequence
Length = 850
Score = 67.0 bits (140), Expect(3) = 4e-026
Identities = 27/52 (51%), Positives = 33/52 (63%)
Frame = +1 / +3
Query: 541 CGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGASTP 696
CG L +FA+ Q+DY+LG NP+ SYVVGFG P + HHRGAS P
Sbjct: 87 CGPNFYTTDVLRKFAKSQLDYILGKNPQKMSYVVGFGKKYPKRVHHRGASIP 242
Score = 63.8 bits (133), Expect(3) = 4e-026
Identities = 27/54 (50%), Positives = 33/54 (61%)
Frame = +1 / +3
Query: 745 WMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLAALA 906
W K NPN L GA+V GPDK+DGF D R N +YTEP N+ + L +LA
Sbjct: 282 WRESKKANPNILVGAMVAGPDKHDGFKDIRTNYNYTEPTLAANAGLVAALISLA 443
Score = 25.4 bits (49), Expect(3) = 4e-026
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +1 / +3
Query: 466 QYVTSTAFLLVVYADLL 516
QYV + AFL +YAD L
Sbjct: 9 QYVVNAAFLASLYADYL 59
>gb|BG418595.1|BG418595 HVSMEk0023G06f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0023G06f, mRNA sequence
Length = 815
Score = 67.0 bits (140), Expect(3) = 4e-026
Identities = 27/52 (51%), Positives = 33/52 (63%)
Frame = +1 / +3
Query: 541 CGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGASTP 696
CG L +FA+ Q+DY+LG NP+ SYVVGFG P + HHRGAS P
Sbjct: 111 CGPNFYTTDVLRKFAKSQLDYILGKNPQKMSYVVGFGKKYPKRVHHRGASIP 266
Score = 63.8 bits (133), Expect(3) = 4e-026
Identities = 27/54 (50%), Positives = 33/54 (61%)
Frame = +1 / +3
Query: 745 WMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLAALA 906
W K NPN L GA+V GPDK+DGF D R N +YTEP N+ + L +LA
Sbjct: 306 WRESKKANPNILVGAMVAGPDKHDGFKDIRTNYNYTEPTLAANAGLVAALISLA 467
Score = 25.4 bits (49), Expect(3) = 4e-026
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +1 / +3
Query: 466 QYVTSTAFLLVVYADLL 516
QYV + AFL +YAD L
Sbjct: 33 QYVVNAAFLASLYADYL 83
>gb|BE601584.3|BE601584 HVSMEh0098M02f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0098M02f, mRNA sequence
Length = 641
Score = 67.0 bits (140), Expect(3) = 4e-026
Identities = 27/52 (51%), Positives = 33/52 (63%)
Frame = +1 / +1
Query: 541 CGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGASTP 696
CG L +FA+ Q+DY+LG NP+ SYVVGFG P + HHRGAS P
Sbjct: 142 CGPNFYTTDVLRKFAKSQLDYILGKNPQKMSYVVGFGKKYPKRVHHRGASIP 297
Score = 63.8 bits (133), Expect(3) = 4e-026
Identities = 27/54 (50%), Positives = 33/54 (61%)
Frame = +1 / +1
Query: 745 WMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLAALA 906
W K NPN L GA+V GPDK+DGF D R N +YTEP N+ + L +LA
Sbjct: 337 WRESKKANPNILVGAMVAGPDKHDGFKDIRTNYNYTEPTLAANAGLVAALISLA 498
Score = 25.4 bits (49), Expect(3) = 4e-026
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +1 / +1
Query: 466 QYVTSTAFLLVVYADLL 516
QYV + AFL +YAD L
Sbjct: 64 QYVVNAAFLASLYADYL 114
>gb|BM097444.2|BM097444 EBro05_SQ003_F03_R root, 3 week, reduced light, cv Optic, EBro05
Hordeum vulgare subsp. vulgare cDNA clone
EBro05_SQ003_F03 5', mRNA sequence
Length = 499
Score = 67.0 bits (140), Expect(3) = 4e-026
Identities = 27/52 (51%), Positives = 33/52 (63%)
Frame = +1 / +1
Query: 541 CGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGASTP 696
CG L +FA+ Q+DY+LG NP+ SYVVGFG P + HHRGAS P
Sbjct: 136 CGPNFYTTDVLRKFAKSQLDYILGKNPQKMSYVVGFGKKYPKRVHHRGASIP 291
Score = 63.8 bits (133), Expect(3) = 4e-026
Identities = 27/54 (50%), Positives = 33/54 (61%)
Frame = +1 / +1
Query: 745 WMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLAALA 906
W K NPN L GA+V GPDK+DGF D R N +YTEP N+ + L +LA
Sbjct: 331 WRESKKANPNILVGAMVAGPDKHDGFKDIRTNYNYTEPTLAANAGLVAALISLA 492
Score = 25.4 bits (49), Expect(3) = 4e-026
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +1 / +1
Query: 466 QYVTSTAFLLVVYADLL 516
QYV + AFL +YAD L
Sbjct: 58 QYVVNAAFLASLYADYL 108
>gb|AW983004.3|AW983004 HVSMEg0004M14f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0004M14f, mRNA sequence
Length = 562
Score = 67.0 bits (140), Expect(3) = 1e-024
Identities = 27/52 (51%), Positives = 33/52 (63%)
Frame = +1 / +1
Query: 541 CGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGASTP 696
CG L +FA+ Q+DY+LG NP+ SYVVGFG P + HHRGAS P
Sbjct: 187 CGPNFYTTDVLRKFAKSQLDYILGKNPQKMSYVVGFGKKYPKRVHHRGASIP 342
Score = 58.8 bits (122), Expect(3) = 1e-024
Identities = 26/54 (48%), Positives = 32/54 (59%)
Frame = +1 / +1
Query: 745 WMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLAALA 906
W K NPN L GA+V GPDK+DGF D R N +YT+P N+ L +LA
Sbjct: 382 WRESKKANPNILVGAMVAGPDKHDGFKDIRTNYNYTKPTLAANAGLGATLISLA 543
Score = 25.4 bits (49), Expect(3) = 1e-024
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +1 / +1
Query: 466 QYVTSTAFLLVVYADLL 516
QYV + AFL +YAD L
Sbjct: 109 QYVVNAAFLASLYADYL 159
>gb|CK122715.1|CK122715 BES1824105i22 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010I225 5-PRIME, mRNA sequence
Length = 776
Score = 67.0 bits (140), Expect(2) = 2e-024
Identities = 30/63 (47%), Positives = 36/63 (57%)
Frame = +1 / +2
Query: 718 VNCGLSFGDWMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLA 897
V+C + W NPN L GAIVGGPD+ D F D+R N TE TY N+ +G LA
Sbjct: 194 VSCRGGYATWYPRKGSNPNLLDGAIVGGPDEYDNFADERNNYEQTEATTYNNAPFMGVLA 373
Query: 898 ALA 906
LA
Sbjct: 374 RLA 382
Score = 66.1 bits (138), Expect(2) = 2e-024
Identities = 26/52 (50%), Positives = 35/52 (67%)
Frame = +1 / +2
Query: 535 VLCGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGAS 690
V C PA + FA+ Q+DY+LG+NPR +SY+VG+G P + HHRGAS
Sbjct: 8 VRCPAGAASPAEIMAFAKSQVDYILGSNPRGTSYMVGYGFIYPLEAHHRGAS 163
>gb|CK123273.1|CK123273 BES1824103c09 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010C093 5-PRIME, mRNA sequence
Length = 594
Score = 67.0 bits (140), Expect(2) = 2e-024
Identities = 30/63 (47%), Positives = 36/63 (57%)
Frame = +1 / +2
Query: 718 VNCGLSFGDWMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLA 897
V+C + W NPN L GAIVGGPD+ D F D+R N TE TY N+ +G LA
Sbjct: 251 VSCRGGYATWYPRKGSNPNLLDGAIVGGPDEYDNFADERNNYEQTEATTYNNAPFMGVLA 430
Query: 898 ALA 906
LA
Sbjct: 431 RLA 439
Score = 66.1 bits (138), Expect(2) = 2e-024
Identities = 26/52 (50%), Positives = 35/52 (67%)
Frame = +1 / +2
Query: 535 VLCGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGAS 690
V C PA + FA+ Q+DY+LG+NPR +SY+VG+G P + HHRGAS
Sbjct: 65 VRCPAGAASPAEIMAFAKSQVDYILGSNPRGTSYMVGYGFIYPLEAHHRGAS 220
>gb|BQ755502.1|BQ755502 EBed07_SQ002_E24_R endosperm, 28 DPA, no treatment, cv Optic,
EBed07 Hordeum vulgare subsp. vulgare cDNA clone
EBed07_SQ002_E24 5', mRNA sequence
Length = 397
Score = 74.4 bits (156), Expect(2) = 4e-024
Identities = 31/62 (50%), Positives = 36/62 (58%)
Frame = +1 / +2
Query: 718 VNCGLSFGDWMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLA 897
+ C F DW A +PNPN LTGAIVGGP+ D F D RGN TE CTY + + A
Sbjct: 173 IGCMQGFDDWFARKRPNPNVLTGAIVGGPNCRDEFRDDRGNYVQTEACTYNTAPMVAVFA 352
Query: 898 AL 903
L
Sbjct: 353 RL 358
Score = 57.4 bits (119), Expect(2) = 4e-024
Identities = 22/45 (48%), Positives = 28/45 (62%)
Frame = +1 / +2
Query: 556 LPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGAS 690
+PP+ L AR Q +Y+LG NP SY+VG+G P HHR AS
Sbjct: 8 VPPSELLALARSQAEYILGRNPLRLSYMVGYGPRYPAHVHHRAAS 142
>gb|AV927273.1|AV927273 AV927273 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd1e17 3', mRNA sequence
Length = 684
Score = 67.0 bits (140), Expect(2) = 8e-024
Identities = 27/52 (51%), Positives = 33/52 (63%)
Frame = +1 / -3
Query: 541 CGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGASTP 696
CG L +FA+ Q+DY+LG NP+ SYVVGFG P + HHRGAS P
Sbjct: 640 CGPNFYTTDVLRKFAKSQLDYILGKNPQKMSYVVGFGKKYPKRVHHRGASIP 485
Score = 63.8 bits (133), Expect(2) = 8e-024
Identities = 27/54 (50%), Positives = 33/54 (61%)
Frame = +1 / -3
Query: 745 WMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLAALA 906
W K NPN L GA+V GPDK+DGF D R N +YTEP N+ + L +LA
Sbjct: 445 WRESKKANPNILVGAMVAGPDKHDGFKDIRTNYNYTEPTLAANAGLVAALISLA 284
>gb|BM099274.2|BM099274 EBes01_SQ002_M04_R embryo sac, 4-6 DPA, no treatment, cv Optic,
EBes01 Hordeum vulgare subsp. vulgare cDNA clone
EBes01_SQ002_M04 5', mRNA sequence
Length = 464
Score = 109 bits (233), Expect = 3e-023
Identities = 45/100 (45%), Positives = 64/100 (64%)
Frame = +1 / +3
Query: 382 CAVLPDTAFHQVFITPGGVIHLRDGANSQYVTSTAFLLVVYADLLLRTGQTVLCGNQPLP 561
C+++P T Q T GG++ +N QYVTS++FLL+ YA+ L+ +TV CG +
Sbjct: 162 CSMVPGTPTDQTTYTRGGLLFKLSDSNMQYVTSSSFLLLTYANYLVSAKKTVSCGGVAVT 341
Query: 562 PARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHR 681
P RL AR+ +DYLLG+NP SY+VG+GA P + HHR
Sbjct: 342 PQRLRAIARRMVDYLLGSNPMGMSYMVGYGAKYPKKLHHR 461
>gb|AV916435.1|AV916435 AV916435 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags17d08 5', mRNA sequence
Length = 607
Score = 67.0 bits (140), Expect(3) = 5e-023
Identities = 27/52 (51%), Positives = 33/52 (63%)
Frame = +1 / +3
Query: 541 CGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGASTP 696
CG L +FA+ Q+DY+LG NP+ SYVVGFG P + HHRGAS P
Sbjct: 303 CGPNFYTTDVLRKFAKSQLDYILGKNPQKMSYVVGFGKKYPKRVHHRGASIP 458
Score = 53.3 bits (110), Expect(3) = 5e-023
Identities = 21/36 (58%), Positives = 24/36 (66%)
Frame = +1 / +3
Query: 745 WMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYT 852
W K NPN L GA+V GPDK+DGF D R N +YT
Sbjct: 498 WRESKKANPNILVGAMVAGPDKHDGFKDIRTNYNYT 605
Score = 25.4 bits (49), Expect(3) = 5e-023
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +1 / +3
Query: 466 QYVTSTAFLLVVYADLL 516
QYV + AFL +YAD L
Sbjct: 225 QYVVNAAFLASLYADYL 275
>gb|BQ460303.1|BQ460303 HA06K13r HA Hordeum vulgare subsp. vulgare cDNA clone HA06K13
5-PRIME, mRNA sequence
Length = 581
Score = 67.0 bits (140), Expect(2) = 7e-023
Identities = 30/63 (47%), Positives = 36/63 (57%)
Frame = +1 / +1
Query: 718 VNCGLSFGDWMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLA 897
V+C + W NPN L GAIVGGPD+ D F D+R N TE TY N+ +G LA
Sbjct: 163 VSCRGGYATWYPRKGSNPNLLDGAIVGGPDEYDNFADERNNYEQTEATTYNNAPFMGVLA 342
Query: 898 ALA 906
LA
Sbjct: 343 RLA 351
Score = 60.6 bits (126), Expect(2) = 7e-023
Identities = 22/37 (59%), Positives = 30/37 (81%)
Frame = +1 / +1
Query: 580 FARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGAS 690
FA+ Q+DY+LG+NPR +SY+VG+G P + HHRGAS
Sbjct: 22 FAKSQVDYILGSNPRGTSYMVGYGFIYPLEAHHRGAS 132
>gb|CK125396.1|CK125396 BES1824107o06 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010O067 5-PRIME, mRNA sequence
Length = 745
Score = 66.1 bits (138), Expect(2) = 1e-022
Identities = 26/52 (50%), Positives = 35/52 (67%)
Frame = +1 / +2
Query: 535 VLCGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGAS 690
V C PA + FA+ Q+DY+LG+NPR +SY+VG+G P + HHRGAS
Sbjct: 425 VRCPAGAASPAEIMAFAKSQVDYILGSNPRGTSYMVGYGFIYPLEAHHRGAS 580
Score = 61.1 bits (127), Expect(2) = 1e-022
Identities = 31/77 (40%), Positives = 42/77 (54%)
Frame = +1 / +2
Query: 301 AQVLLAEFNMTSAGGAQNFKSQADNFVCAVLPDTAFHQVFITPGGVIHLRDGANSQYVTS 480
A++LL A Q ++ +AD FVCA L V TPGG++H + N Q+VTS
Sbjct: 188 AKILLHGKAGAHAAVLQRYRQKADFFVCASLGKQGSGNVKRTPGGLLHHQKWNNLQFVTS 367
Query: 481 TAFLLVVYADLLLRTGQ 531
+FLL Y+D L GQ
Sbjct: 368 GSFLLAAYSDSLAAAGQ 418
>gb|BQ760319.1|BQ760319 EBpi07_SQ003_L24_R pistil, 12 DPA, no treatment, cv Optic, EBpi07
Hordeum vulgare subsp. vulgare cDNA clone
EBpi07_SQ003_L24 5', mRNA sequence
Length = 620
Score = 63.4 bits (132), Expect(2) = 3e-022
Identities = 26/57 (45%), Positives = 35/57 (61%)
Frame = +1 / +1
Query: 745 WMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLAALAVRG 915
W KPNP+ + GA+V GPD++DGF D R N +YTEP N+ + L AL+ G
Sbjct: 223 WRDTKKPNPHIIVGAMVAGPDRHDGFKDIRKNRNYTEPTLAGNAGLVAALVALSGEG 393
Score = 62.5 bits (130), Expect(2) = 3e-022
Identities = 25/42 (59%), Positives = 29/42 (69%)
Frame = +1 / +1
Query: 571 LHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGASTP 696
L FAR QM+Y+LG NP SYVVG G + P + HHRGAS P
Sbjct: 55 LRSFARTQMEYILGKNPLKMSYVVGHGKHYPKRVHHRGASIP 180
>gb|BJ467008.1|BJ467008 BJ467008 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags35i04 3', mRNA sequence
Length = 786
Score = 64.8 bits (135), Expect(2) = 5e-022
Identities = 27/55 (49%), Positives = 36/55 (65%)
Frame = +1 / -3
Query: 532 TVLCGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGASTP 696
TV CG+ + P L A++Q+DYLLG NP SY+VG+G P + HHRG+S P
Sbjct: 781 TVNCGDISVGPDTLRLQAKKQIDYLLGDNPMKMSYMVGYGDRYPQRIHHRGSSLP 617
Score = 60.2 bits (125), Expect(2) = 5e-022
Identities = 24/45 (53%), Positives = 31/45 (68%)
Frame = +1 / -3
Query: 763 PNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLA 897
PNPN L GAIVGGP ++D + D R + +EP TYIN+ +G LA
Sbjct: 550 PNPNPLVGAIVGGPGEDDSYGDDRADYRKSEPTTYINAPLVGVLA 416
>gb|BU985662.1|BU985662 HF08E02r HF Hordeum vulgare subsp. vulgare cDNA clone HF08E02
5-PRIME, mRNA sequence
Length = 583
Score = 67.0 bits (140), Expect(4) = 6e-022
Identities = 27/52 (51%), Positives = 33/52 (63%)
Frame = +1 / +3
Query: 541 CGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGASTP 696
CG L +FA+ Q+DY+LG NP+ SYVVGFG P + HHRGAS P
Sbjct: 312 CGPNFYTTDVLRKFAKSQLDYILGKNPQKMSYVVGFGKKYPKRVHHRGASIP 467
Score = 40.9 bits (83), Expect(4) = 6e-022
Identities = 15/25 (60%), Positives = 17/25 (68%)
Frame = +1 / +3
Query: 745 WMAPDKPNPNELTGAIVGGPDKNDG 819
W K NPN L GA+V GPDK+DG
Sbjct: 507 WRESKKANPNILVGAMVAGPDKHDG 581
Score = 30.4 bits (60), Expect(4) = 6e-022
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = +1 / +3
Query: 274 FSWDLKFPGAQVLLAEFNMTSAGG 345
F+WD K PG+QVLL+ + + G
Sbjct: 24 FTWDDKLPGSQVLLSRLRLFLSPG 95
Score = 25.4 bits (49), Expect(4) = 6e-022
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +1 / +3
Query: 466 QYVTSTAFLLVVYADLL 516
QYV + AFL +YAD L
Sbjct: 234 QYVVNAAFLASLYADYL 284
>gb|CB875548.1|CB875548 HX08K06w HX Hordeum vulgare subsp. vulgare cDNA clone HX08K06
3-PRIME, mRNA sequence
Length = 620
Score = 63.4 bits (132), Expect(2) = 9e-022
Identities = 26/57 (45%), Positives = 35/57 (61%)
Frame = +1 / -3
Query: 745 WMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLAALAVRG 915
W KPNP+ + GA+V GPD++DGF D R N +YTEP N+ + L AL+ G
Sbjct: 456 WRDTKKPNPHIIVGAMVAGPDRHDGFKDIRKNRNYTEPTLAGNAGLVAALVALSGEG 286
Score = 60.6 bits (126), Expect(2) = 9e-022
Identities = 24/39 (61%), Positives = 28/39 (71%)
Frame = +1 / -3
Query: 580 FARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGASTP 696
FAR QM+Y+LG NP SYVVG G + P + HHRGAS P
Sbjct: 615 FARTQMEYILGKNPLKMSYVVGHGKHYPKRVHHRGASIP 499
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 422,765,745
Number of Sequences: 312970
Number of extensions: 7813017
Number of successful extensions: 90026
Number of sequences better than 1.0e-020: 39
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 14292442998
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)