TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3670867.2.1
(790 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA024243.1|CA024243 HZ48M07r HZ Hordeum vulgare subsp. v... 167 7e-041
gb|BM372253.2|BM372253 EBro03_SQ004_H03_R root, 3 week, wat... 148 4e-040
gb|AL503076.1|AL503076 AL503076 Hordeum vulgare Barke roots... 148 4e-040
gb|CA018552.1|CA018552 HV08P07r HV Hordeum vulgare subsp. v... 113 7e-030
gb|CA006291.1|CA006291 HU14N03u HU Hordeum vulgare subsp. v... 110 9e-024
gb|CA030254.1|CA030254 HX06I12r HX Hordeum vulgare subsp. v... 109 2e-023
gb|BI957153.1|BI957153 HVSMEn0007M22f Hordeum vulgare rachi... 107 8e-023
gb|BQ472774.1|BQ472774 HB01I09r BC Hordeum vulgare subsp. v... 105 2e-022
gb|BU996502.1|BU996502 HM13N02r HM Hordeum vulgare subsp. v... 104 8e-022
>gb|CA024243.1|CA024243 HZ48M07r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ48M07
5-PRIME, mRNA sequence
Length = 518
Score = 167 bits (359), Expect = 7e-041
Identities = 66/95 (69%), Positives = 75/95 (78%)
Frame = -2 / +2
Query: 633 PLALSAWMCVLSTLQSALLVSFLLPDPSAWRIHSLFELSCCVFSGVFGSGVTFYLQSWCI 454
PL LSAWMC LS Q ALL SF +PD AW+I+SLFEL C+F+G GSGVTFYLQSWCI
Sbjct: 233 PLTLSAWMCFLSVAQMALLSSFAVPDMDAWKINSLFELMGCIFAGAVGSGVTFYLQSWCI 412
Query: 453 SVRGPLYSAMFKPLCTVITTVFAAAVLREELHVGS 349
+VRGPLYSAMF PLCTV+TTV A L E+ H+GS
Sbjct: 413 TVRGPLYSAMFNPLCTVVTTVLATIFLHEQPHIGS 517
Score = 129 bits (277), Expect = 1e-029
Identities = 60/95 (63%), Positives = 63/95 (66%)
Frame = +2 / -3
Query: 350 LPTCSSSRRTAAANTVVITVQSGLNMAE*SGPLTEMHHDCR*NVTPDPNTPEKTQQESSK 529
LP C S R A+TVV TVQ GLNMAE* GPLT MHHDCR*NVTPDP P K Q SSK
Sbjct: 516 LPMCGCSWRKMVASTVVTTVQRGLNMAE*RGPLTVMHHDCR*NVTPDPTAPAKMQPMSSK 337
Query: 530 SEWILQALGSGRRKETSSADWSVDRTHIHADRASG 634
SE I QA SG KE S+A + DR HI AD G
Sbjct: 336 SELIFQASMSGTAKELSNAICATDRKHIQADSVRG 232
Score = 104 bits (221), Expect = 8e-022
Identities = 48/73 (65%), Positives = 49/73 (67%)
Frame = +1 / -2
Query: 412 ERFEHGGVERXPHGDAPRLQVERDAGPEHAREDAAGELEERVDPPGAGVRQEEGDEQRGL 591
E EHG VER PH APRLQVER P A EDAA ELEERVD PG VR EG EQR L
Sbjct: 454 EGVEHGRVERPPHRYAPRLQVERHP*PNRAGEDAAHELEERVDLPGVHVRHREGAEQRHL 275
Query: 592 ERGQDAHPRRQSQ 630
GQ+AHP RQ Q
Sbjct: 274 RHGQEAHPGRQRQ 236
>gb|BM372253.2|BM372253 EBro03_SQ004_H03_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ004_H03 5', mRNA sequence
Length = 643
Score = 148 bits (317), Expect(2) = 4e-040
Identities = 55/89 (61%), Positives = 71/89 (79%)
Frame = -2 / +2
Query: 546 WRIHSLFELSCCVFSGVFGSGVTFYLQSWCISVRGPLYSAMFKPLCTVITTVFAAAVLRE 367
W++ S++EL C ++ GVF SG F++QSWCISV+GPLYSA+F PL VITT+ + L E
Sbjct: 125 WKLASIWELPCILYGGVFASGANFFMQSWCISVKGPLYSAIFTPLSAVITTILSTLFLHE 304
Query: 366 ELHVGSLLGAIAVIAGLYVVLWGKAGDAN 280
ELH+GS+LGAI +I GLYVVLWGKA DA+
Sbjct: 305 ELHIGSVLGAIVIILGLYVVLWGKADDAS 391
Score = 36.8 bits (74), Expect(2) = 4e-040
Identities = 13/23 (56%), Positives = 18/23 (78%)
Frame = -2 / +2
Query: 633 PLALSAWMCVLSTLQSALLVSFL 565
PL+L+ WMC L+TLQ A++ FL
Sbjct: 35 PLSLATWMCFLATLQCAVMAFFL 103
>gb|AL503076.1|AL503076 AL503076 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW09K03u 3', mRNA sequence
Length = 601
Score = 148 bits (317), Expect(2) = 4e-040
Identities = 55/89 (61%), Positives = 71/89 (79%)
Frame = -2 / -1
Query: 546 WRIHSLFELSCCVFSGVFGSGVTFYLQSWCISVRGPLYSAMFKPLCTVITTVFAAAVLRE 367
W++ S++EL C ++ GVF SG F++QSWCISV+GPLYSA+F PL VITT+ + L E
Sbjct: 448 WKLASIWELPCILYGGVFASGANFFMQSWCISVKGPLYSAIFTPLSAVITTILSTLFLHE 269
Query: 366 ELHVGSLLGAIAVIAGLYVVLWGKAGDAN 280
ELH+GS+LGAI +I GLYVVLWGKA DA+
Sbjct: 268 ELHIGSVLGAIVIILGLYVVLWGKADDAS 182
Score = 36.8 bits (74), Expect(2) = 4e-040
Identities = 13/23 (56%), Positives = 18/23 (78%)
Frame = -2 / -1
Query: 633 PLALSAWMCVLSTLQSALLVSFL 565
PL+L+ WMC L+TLQ A++ FL
Sbjct: 538 PLSLATWMCFLATLQCAVMAFFL 470
>gb|CA018552.1|CA018552 HV08P07r HV Hordeum vulgare subsp. vulgare cDNA clone HV08P07
5-PRIME, mRNA sequence
Length = 575
Score = 113 bits (242), Expect(2) = 7e-030
Identities = 41/73 (56%), Positives = 56/73 (76%)
Frame = -2 / +3
Query: 546 WRIHSLFELSCCVFSGVFGSGVTFYLQSWCISVRGPLYSAMFKPLCTVITTVFAAAVLRE 367
W++ S++EL C ++ GVF SG F++QSWCISV+GPLYSA+F PL VITT+ + L E
Sbjct: 357 WKLASIWELPCILYGGVFASGANFFMQSWCISVKGPLYSAIFTPLSAVITTILSTLFLHE 536
Query: 366 ELHVGSLLGAIAV 328
ELH+ S+LGAI +
Sbjct: 537 ELHIXSVLGAIVI 575
Score = 36.8 bits (74), Expect(2) = 7e-030
Identities = 13/23 (56%), Positives = 18/23 (78%)
Frame = -2 / +3
Query: 633 PLALSAWMCVLSTLQSALLVSFL 565
PL+L+ WMC L+TLQ A++ FL
Sbjct: 267 PLSLATWMCFLATLQCAVMAFFL 335
>gb|CA006291.1|CA006291 HU14N03u HU Hordeum vulgare subsp. vulgare cDNA clone HU14N03
3-PRIME, mRNA sequence
Length = 582
Score = 110 bits (235), Expect = 9e-024
Identities = 46/121 (38%), Positives = 75/121 (61%)
Frame = -2 / -1
Query: 630 LALSAWMCVLSTLQSALLVSFLLPDPSAWRIHSLFELSCCVFSGVFGSGVTFYLQSWCIS 451
++ +A+M + S+LQ+ L S W + +++ +F+GV SG+ + L +WCI
Sbjct: 549 ISSTAFMALFSSLQAGALAVTTQRRLSVWLLRGSIQIATVLFAGVGVSGIGYVLMTWCIE 370
Query: 450 VRGPLYSAMFKPLCTVITTVFAAAVLREELHVGSLLGAIAVIAGLYVVLWGKAGDANKGG 271
RGP+++A F PL +I V VL E+L+VGS++GA VI GLY++LWGK+ +A+
Sbjct: 369 KRGPVFTAGFLPLIQIIAGVLDLLVLHEQLYVGSVVGAALVIGGLYLLLWGKSKEASSAA 190
Query: 270 V 268
V
Sbjct: 189 V 187
>gb|CA030254.1|CA030254 HX06I12r HX Hordeum vulgare subsp. vulgare cDNA clone HX06I12
5-PRIME, mRNA sequence
Length = 651
Score = 109 bits (233), Expect = 2e-023
Identities = 39/113 (34%), Positives = 71/113 (62%)
Frame = -2 / +1
Query: 630 LALSAWMCVLSTLQSALLVSFLLPDPSAWRIHSLFELSCCVFSGVFGSGVTFYLQSWCIS 451
L+++++ C +Q ++ +F D AW HS E+ +++G SGV F +Q WCI
Sbjct: 304 LSVTSYTCFFGVIQFLIIAAFFERDAGAWVFHSGSEVFTILYAGFIASGVAFAVQIWCIE 483
Query: 450 VRGPLYSAMFKPLCTVITTVFAAAVLREELHVGSLLGAIAVIAGLYVVLWGKA 292
GP++ A+++P+ T++ + A+ L E+ ++G ++GA +I GLY+VLWGK+
Sbjct: 484 RGGPVFVAVYQPVQTLVVAIMASLTLGEKFYLGGIIGAALIITGLYLVLWGKS 642
>gb|BI957153.1|BI957153 HVSMEn0007M22f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0007M22f, mRNA sequence
Length = 1133
Score = 107 bits (228), Expect = 8e-023
Identities = 38/118 (32%), Positives = 72/118 (61%)
Frame = -2 / +3
Query: 630 LALSAWMCVLSTLQSALLVSFLLPDPSAWRIHSLFELSCCVFSGVFGSGVTFYLQSWCIS 451
L+++++ C +Q ++ +F D AW HS E+ +++G SGV F +Q WCI
Sbjct: 183 LSVTSYTCFFGVIQFLIIAAFFERDAGAWVFHSGSEVFTILYAGFIASGVAFAVQIWCIE 362
Query: 450 VRGPLYSAMFKPLCTVITTVFAAAVLREELHVGSLLGAIAVIAGLYVVLWGKAGDANK 277
GP++ A+++P+ T++ + A L E+ ++ ++GA +I GLY+VLWGK+ + ++
Sbjct: 363 RGGPVFVAVYQPVQTLVVAIMACLTLGEKFYLSGIIGAALIITGLYLVLWGKSEERSR 536
>gb|BQ472774.1|BQ472774 HB01I09r BC Hordeum vulgare subsp. vulgare cDNA clone HB01I09
5-PRIME, mRNA sequence
Length = 660
Score = 105 bits (225), Expect = 2e-022
Identities = 41/118 (34%), Positives = 69/118 (58%)
Frame = -2 / +2
Query: 639 TSPLALSAWMCVLSTLQSALLVSFLLPDPSAWRIHSLFELSCCVFSGVFGSGVTFYLQSW 460
+SP + M ++++Q +++ SAW + L +++GV SG+ + SW
Sbjct: 254 SSPYTTTVLMAAMASVQCSVIALAAERRLSAWALGFDIRLIGSLYAGVVASGIVIAVMSW 433
Query: 459 CISVRGPLYSAMFKPLCTVITTVFAAAVLREELHVGSLLGAIAVIAGLYVVLWGKAGD 286
CI VRGP++ +MF P+ +I V +L E++ VGS++GA+ ++ GLY VLWGK D
Sbjct: 434 CIQVRGPVFVSMFSPMMLIIVAVVGWGILGEKIRVGSVIGAVLIVVGLYTVLWGKGRD 607
>gb|BU996502.1|BU996502 HM13N02r HM Hordeum vulgare subsp. vulgare cDNA clone HM13N02
5-PRIME, mRNA sequence
Length = 622
Score = 104 bits (221), Expect = 8e-022
Identities = 45/104 (43%), Positives = 63/104 (60%)
Frame = -2 / +2
Query: 609 CVLSTLQSALLVSFLLPDPSAWRIHSLFELSCCVFSGVFGSGVTFYLQSWCISVRGPLYS 430
CV T+Q+ ++ + P AW + +L V+SGVF +G +F L W +S RGP Y
Sbjct: 311 CVSGTVQAVVIGVMIDRRPMAWALKWNLQLLTVVYSGVFNTGFSFCLILWAVSRRGPTYP 490
Query: 429 AMFKPLCTVITTVFAAAVLREELHVGSLLGAIAVIAGLYVVLWG 298
+MF L +IT V + +L +L VGSLLGA+ +IAGLY LWG
Sbjct: 491 SMFNSLSLIITMVLDSVLLGTDLSVGSLLGAMLIIAGLYAFLWG 622
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 351,730,790
Number of Sequences: 312970
Number of extensions: 5932894
Number of successful extensions: 103681
Number of sequences better than 1.0e-020: 12
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 8841785190
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)