TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3510875.2.1
(707 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ471435.1|BQ471435 HV02G18r HV Hordeum vulgare subsp. v... 172 3e-061
gb|AL507467.1|AL507467 AL507467 Hordeum vulgare Barke devel... 177 4e-061
gb|BQ471542.1|BQ471542 HV02L22r HV Hordeum vulgare subsp. v... 177 4e-061
gb|BQ763415.1|BQ763415 EBro02_SQ008_F07_R root, 3 week, hyd... 177 8e-061
gb|BM373485.2|BM373485 EBma04_SQ004_J22_R maternal, 10 DPA,... 177 8e-061
gb|BQ471411.1|BQ471411 HV02F17r HV Hordeum vulgare subsp. v... 175 1e-060
gb|BM371288.2|BM371288 EBma08_SQ002_C11_R maternal, 28 DPA,... 167 1e-059
gb|BQ763387.1|BQ763387 EBro02_SQ008_O04_R root, 3 week, hyd... 167 1e-059
gb|BE193614.2|BE193614 HVSMEh0081O22f Hordeum vulgare 5-45 ... 167 1e-059
gb|BI957678.1|BI957678 HVSMEn0010J22f Hordeum vulgare rachi... 162 4e-058
gb|BU979623.1|BU979623 HA16L23r HA Hordeum vulgare subsp. v... 160 1e-057
gb|BU981234.1|BU981234 HA23A06r HA Hordeum vulgare subsp. v... 159 3e-057
gb|BQ459061.1|BQ459061 HA02H05r HA Hordeum vulgare subsp. v... 163 4e-057
gb|BI960172.1|BI960172 HVSMEn0023I14f Hordeum vulgare rachi... 112 6e-057
gb|BG417730.2|BG417730 HVSMEk0019E17f Hordeum vulgare testa... 161 1e-056
gb|BG343533.2|BG343533 HVSMEg0006F02f Hordeum vulgare pre-a... 153 4e-054
gb|BU982763.1|BU982763 HA27I15r HA Hordeum vulgare subsp. v... 148 5e-054
gb|AV916732.1|AV916732 AV916732 K. Sato unpublished cDNA li... 177 3e-051
gb|AV922026.1|AV922026 AV922026 K. Sato unpublished cDNA li... 177 3e-051
gb|BF625296.2|BF625296 HVSMEa0008E19f Hordeum vulgare seedl... 112 5e-051
gb|BQ664296.1|BQ664296 HV02L22u HV Hordeum vulgare subsp. v... 177 1e-050
gb|BI960628.1|BI960628 HVSMEn0025G23f Hordeum vulgare rachi... 125 1e-045
gb|BU982674.1|BU982674 HA27E09r HA Hordeum vulgare subsp. v... 116 3e-044
gb|AL510732.1|AL510732 AL510732 Hordeum vulgare Barke devel... 177 8e-044
gb|BG343276.2|BG343276 HVSMEg0005E17f Hordeum vulgare pre-a... 113 4e-042
gb|AL502849.1|AL502849 AL502849 Hordeum vulgare Barke roots... 168 3e-041
gb|BM444860.1|BM444860 EBem09_SQ006_L09_R embryo, 1 Day ger... 167 5e-041
gb|CV056595.1|CV056595 BNEL19E4 Barley EST endosperm librar... 167 5e-041
gb|BI958849.1|BI958849 HVSMEn0016O03f Hordeum vulgare rachi... 165 3e-040
gb|CV054743.1|CV054743 BNEL112g9 Barley EST endosperm libra... 159 2e-038
gb|AJ433789.1|AJ433789 AJ433789 S00002 Hordeum vulgare subs... 155 3e-037
gb|BQ760080.1|BQ760080 EBpi07_SQ003_K06_R pistil, 12 DPA, n... 83 1e-033
gb|CA028103.1|CA028103 HZ61A03r HZ Hordeum vulgare subsp. v... 100 6e-029
gb|BF065894.2|BF065894 HV_CEb0014E14f Hordeum vulgare seedl... 99 1e-028
gb|BF064827.1|BF064827 HV_CEb0019M01f Hordeum vulgare seedl... 90 4e-026
gb|BG300692.2|BG300692 HVSMEb0018A09f Hordeum vulgare seedl... 106 8e-026
gb|CA026158.1|CA026158 HZ55B18r HZ Hordeum vulgare subsp. v... 80 1e-025
gb|AV922971.1|AV922971 AV922971 K. Sato unpublished cDNA li... 100 5e-025
gb|CA029181.1|CA029181 HZ64H06r HZ Hordeum vulgare subsp. v... 78 5e-025
gb|BI958606.1|BI958606 HVSMEn0015N17f Hordeum vulgare rachi... 98 3e-024
gb|BE215009.2|BE215009 HV_CEb0005I10f Hordeum vulgare seedl... 89 8e-024
gb|BI959140.1|BI959140 HVSMEn0018F14f Hordeum vulgare rachi... 89 1e-021
gb|BQ768104.1|BQ768104 EBro08_SQ010_O16_R root, 3 week, dro... 101 3e-021
gb|BF620088.2|BF620088 HVSMEc0011N11f Hordeum vulgare seedl... 100 4e-021
gb|BI958965.1|BI958965 HVSMEn0017G24f Hordeum vulgare rachi... 87 5e-021
gb|BF623292.1|BF623292 HVSMEa0015C23f Hordeum vulgare seedl... 73 5e-021
>gb|BQ471435.1|BQ471435 HV02G18r HV Hordeum vulgare subsp. vulgare cDNA clone HV02G18
5-PRIME, mRNA sequence
Length = 572
Score = 172 bits (371), Expect(2) = 3e-061
Identities = 74/92 (80%), Positives = 82/92 (89%)
Frame = -2 / +2
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
E+GRAQGTYTFADQ TFGLLM MNFVFTAG++ GS L+I GRNEVLS VREMSIVGG+GK
Sbjct: 281 EIGRAQGTYTFADQATFGLLMNMNFVFTAGDYKGSGLTIYGRNEVLSAVREMSIVGGTGK 460
Query: 358 FRMARGYVQAHTIDSGATSGTTVVQYTVNVKA 263
FRMARGYV+A T+DSGA SG TVV+YTV VKA
Sbjct: 461 FRMARGYVEARTMDSGAKSGETVVEYTVYVKA 556
Score = 132 bits (282), Expect(2) = 4e-048
Identities = 62/92 (67%), Positives = 65/92 (70%)
Frame = +3 / -3
Query: 264 ALTLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPL 443
ALT TVY TTV P++APESMV A T PRAMRNLP+PPTMLI P M LPL
Sbjct: 555 ALT*TVYSTTVSPDLAPESMVRAST*PRAMRNLPVPPTMLISLTADSTSLRP*MVRPLPL 376
Query: 444 CSPAVNTKFMTMRRPNVCWSANV*VPWARPTS 539
SPAV TKFM MRRP V WSANV*VP ARP S
Sbjct: 375 *SPAVKTKFMFMRRPKVAWSANV*VPCARPIS 280
Score = 82.6 bits (174), Expect(2) = 3e-061
Identities = 33/50 (66%), Positives = 39/50 (78%)
Frame = -2 / +2
Query: 706 GLTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
GLT ++Y+HDV+AGT+PTA+RIAQ A FGAVVAIDDPLTTGP
Sbjct: 101 GLTKFKLYWHDVLAGTSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTGP 250
Score = 79.4 bits (167), Expect(2) = 4e-048
Identities = 33/50 (66%), Positives = 41/50 (82%)
Frame = +3 / -3
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVVNP 707
GPVVSGSSMATTAPK V+++L DA CA+R AVG VPA+TS +Y+ +V P
Sbjct: 249 GPVVSGSSMATTAPKKVEELLVDAACAIRIAVGLVPASTSCQYSLNLVRP 100
>gb|AL507467.1|AL507467 AL507467 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY05N10V 5',
mRNA sequence
Length = 668
Score = 177 bits (380), Expect(2) = 4e-061
Identities = 76/92 (82%), Positives = 83/92 (90%)
Frame = -2 / +3
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
EVGRAQGTYTFADQ TFGLLM MNFVFTAGE+ GS+L+I GRNEVLS VREMSI+GG+GK
Sbjct: 315 EVGRAQGTYTFADQATFGLLMNMNFVFTAGEYKGSSLTIYGRNEVLSAVREMSILGGTGK 494
Query: 358 FRMARGYVQAHTIDSGATSGTTVVQYTVNVKA 263
FRMARGYVQA T+DSGA SG TVV+YTV VKA
Sbjct: 495 FRMARGYVQASTVDSGANSGETVVEYTVYVKA 590
Score = 134 bits (288), Expect(2) = 2e-048
Identities = 62/92 (67%), Positives = 65/92 (70%)
Frame = +3 / -2
Query: 264 ALTLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPL 443
ALT TVY TTV PE+APES V A T PRAMRNLP+PP MLI P M +LPL
Sbjct: 589 ALTYTVYSTTVSPELAPESTVLAWT*PRAMRNLPVPPRMLISLTAESTSLRP*MVRLLPL 410
Query: 444 CSPAVNTKFMTMRRPNVCWSANV*VPWARPTS 539
SPAVNTKFM MR P V WSANV*VP ARPTS
Sbjct: 409 YSPAVNTKFMFMRSPKVAWSANV*VPCARPTS 314
Score = 78.0 bits (164), Expect(2) = 2e-048
Identities = 33/48 (68%), Positives = 38/48 (79%)
Frame = +3 / -2
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVV 701
G VVSGSSMATTAPK V+++L DA CA+R AVG PA TSWKYT +V
Sbjct: 283 GLVVSGSSMATTAPKKVEELLVDAACAIRIAVGLFPARTSWKYTLNLV 140
Score = 78.0 bits (164), Expect(2) = 4e-061
Identities = 32/49 (65%), Positives = 36/49 (73%)
Frame = -2 / +3
Query: 703 LTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
LT +VYFHDV+AG +PTA+RIAQ A FGAVVAIDDPLTT P
Sbjct: 138 LTKFKVYFHDVLAGKSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTSP 284
>gb|BQ471542.1|BQ471542 HV02L22r HV Hordeum vulgare subsp. vulgare cDNA clone HV02L22
5-PRIME, mRNA sequence
Length = 565
Score = 177 bits (380), Expect(2) = 4e-061
Identities = 76/92 (82%), Positives = 83/92 (90%)
Frame = -2 / +2
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
EVGRAQGTYTFADQ TFGLLM MNFVFTAGE+ GS+L+I GRNEVLS VREMSI+GG+GK
Sbjct: 290 EVGRAQGTYTFADQATFGLLMNMNFVFTAGEYKGSSLTIYGRNEVLSAVREMSILGGTGK 469
Query: 358 FRMARGYVQAHTIDSGATSGTTVVQYTVNVKA 263
FRMARGYVQA T+DSGA SG TVV+YTV VKA
Sbjct: 470 FRMARGYVQASTVDSGANSGETVVEYTVYVKA 565
Score = 134 bits (288), Expect(2) = 2e-048
Identities = 62/92 (67%), Positives = 65/92 (70%)
Frame = +3 / -2
Query: 264 ALTLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPL 443
ALT TVY TTV PE+APES V A T PRAMRNLP+PP MLI P M +LPL
Sbjct: 564 ALTYTVYSTTVSPELAPESTVLAWT*PRAMRNLPVPPRMLISLTAESTSLRP*MVRLLPL 385
Query: 444 CSPAVNTKFMTMRRPNVCWSANV*VPWARPTS 539
SPAVNTKFM MR P V WSANV*VP ARPTS
Sbjct: 384 YSPAVNTKFMFMRSPKVAWSANV*VPCARPTS 289
Score = 78.0 bits (164), Expect(2) = 2e-048
Identities = 33/48 (68%), Positives = 38/48 (79%)
Frame = +3 / -2
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVV 701
G VVSGSSMATTAPK V+++L DA CA+R AVG PA TSWKYT +V
Sbjct: 258 GLVVSGSSMATTAPKKVEELLVDAACAIRIAVGLFPARTSWKYTLNLV 115
Score = 78.0 bits (164), Expect(2) = 4e-061
Identities = 32/49 (65%), Positives = 36/49 (73%)
Frame = -2 / +2
Query: 703 LTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
LT +VYFHDV+AG +PTA+RIAQ A FGAVVAIDDPLTT P
Sbjct: 113 LTKFKVYFHDVLAGKSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTSP 259
>gb|BQ763415.1|BQ763415 EBro02_SQ008_F07_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ008_F07 5', mRNA sequence
Length = 660
Score = 177 bits (380), Expect(2) = 8e-061
Identities = 76/92 (82%), Positives = 83/92 (90%)
Frame = -2 / +2
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
EVGRAQGTYTFADQ TFGLLM MNFVFTAGE+ GS+L+I GRNEVLS VREMSI+GG+GK
Sbjct: 287 EVGRAQGTYTFADQATFGLLMNMNFVFTAGEYKGSSLTIYGRNEVLSAVREMSILGGTGK 466
Query: 358 FRMARGYVQAHTIDSGATSGTTVVQYTVNVKA 263
FRMARGYVQA T+DSGA SG TVV+YTV VKA
Sbjct: 467 FRMARGYVQASTVDSGANSGETVVEYTVYVKA 562
Score = 134 bits (288), Expect(2) = 2e-048
Identities = 62/92 (67%), Positives = 65/92 (70%)
Frame = +3 / -1
Query: 264 ALTLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPL 443
ALT TVY TTV PE+APES V A T PRAMRNLP+PP MLI P M +LPL
Sbjct: 561 ALTYTVYSTTVSPELAPESTVLAWT*PRAMRNLPVPPRMLISLTAESTSLRP*MVRLLPL 382
Query: 444 CSPAVNTKFMTMRRPNVCWSANV*VPWARPTS 539
SPAVNTKFM MR P V WSANV*VP ARPTS
Sbjct: 381 YSPAVNTKFMFMRSPKVAWSANV*VPCARPTS 286
Score = 78.0 bits (164), Expect(2) = 2e-048
Identities = 33/48 (68%), Positives = 38/48 (79%)
Frame = +3 / -1
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVV 701
G VVSGSSMATTAPK V+++L DA CA+R AVG PA TSWKYT +V
Sbjct: 255 GLVVSGSSMATTAPKKVEELLVDAACAIRIAVGLFPARTSWKYTLNLV 112
Score = 77.1 bits (162), Expect(2) = 8e-061
Identities = 31/49 (63%), Positives = 36/49 (73%)
Frame = -2 / +2
Query: 703 LTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
+T +VYFHDV+AG +PTA+RIAQ A FGAVVAIDDPLTT P
Sbjct: 110 MTKFKVYFHDVLAGKSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTSP 256
>gb|BM373485.2|BM373485 EBma04_SQ004_J22_R maternal, 10 DPA, no treatment, cv Optic, EBma04
Hordeum vulgare subsp. vulgare cDNA clone
EBma04_SQ004_J22 5', mRNA sequence
Length = 634
Score = 177 bits (380), Expect(2) = 8e-061
Identities = 76/92 (82%), Positives = 83/92 (90%)
Frame = -2 / +3
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
EVGRAQGTYTFADQ TFGLLM MNFVFTAGE+ GS+L+I GRNEVLS VREMSI+GG+GK
Sbjct: 285 EVGRAQGTYTFADQATFGLLMNMNFVFTAGEYKGSSLTIYGRNEVLSAVREMSILGGTGK 464
Query: 358 FRMARGYVQAHTIDSGATSGTTVVQYTVNVKA 263
FRMARGYVQA T+DSGA SG TVV+YTV VKA
Sbjct: 465 FRMARGYVQASTVDSGANSGETVVEYTVYVKA 560
Score = 134 bits (288), Expect(2) = 2e-048
Identities = 62/92 (67%), Positives = 65/92 (70%)
Frame = +3 / -1
Query: 264 ALTLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPL 443
ALT TVY TTV PE+APES V A T PRAMRNLP+PP MLI P M +LPL
Sbjct: 559 ALTYTVYSTTVSPELAPESTVLAWT*PRAMRNLPVPPRMLISLTAESTSLRP*MVRLLPL 380
Query: 444 CSPAVNTKFMTMRRPNVCWSANV*VPWARPTS 539
SPAVNTKFM MR P V WSANV*VP ARPTS
Sbjct: 379 YSPAVNTKFMFMRSPKVAWSANV*VPCARPTS 284
Score = 78.0 bits (164), Expect(2) = 2e-048
Identities = 33/48 (68%), Positives = 38/48 (79%)
Frame = +3 / -1
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVV 701
G VVSGSSMATTAPK V+++L DA CA+R AVG PA TSWKYT +V
Sbjct: 253 GLVVSGSSMATTAPKKVEELLVDAACAIRIAVGLFPARTSWKYTLNLV 110
Score = 77.1 bits (162), Expect(2) = 8e-061
Identities = 31/49 (63%), Positives = 36/49 (73%)
Frame = -2 / +3
Query: 703 LTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
+T +VYFHDV+AG +PTA+RIAQ A FGAVVAIDDPLTT P
Sbjct: 108 MTKFKVYFHDVLAGKSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTSP 254
>gb|BQ471411.1|BQ471411 HV02F17r HV Hordeum vulgare subsp. vulgare cDNA clone HV02F17
5-PRIME, mRNA sequence
Length = 572
Score = 175 bits (376), Expect(2) = 1e-060
Identities = 75/91 (82%), Positives = 82/91 (90%)
Frame = -2 / +2
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
EVGRAQGTYTFADQ TFGLLM MNFVFTAGE+ GS+L+I GRNEVLS VREMSI+GG+GK
Sbjct: 299 EVGRAQGTYTFADQATFGLLMNMNFVFTAGEYKGSSLTIYGRNEVLSAVREMSILGGTGK 478
Query: 358 FRMARGYVQAHTIDSGATSGTTVVQYTVNVK 266
FRMARGYVQA T+DSGA SG TVV+YTV VK
Sbjct: 479 FRMARGYVQASTVDSGANSGETVVEYTVYVK 571
Score = 133 bits (284), Expect(2) = 6e-048
Identities = 61/91 (67%), Positives = 64/91 (70%)
Frame = +3 / -3
Query: 267 LTLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPLC 446
LT TVY TTV PE+APES V A T PRAMRNLP+PP MLI P M +LPL
Sbjct: 570 LTYTVYSTTVSPELAPESTVLAWT*PRAMRNLPVPPRMLISLTAESTSLRP*MVRLLPLY 391
Query: 447 SPAVNTKFMTMRRPNVCWSANV*VPWARPTS 539
SPAVNTKFM MR P V WSANV*VP ARPTS
Sbjct: 390 SPAVNTKFMFMRSPKVAWSANV*VPCARPTS 298
Score = 78.0 bits (164), Expect(2) = 6e-048
Identities = 33/48 (68%), Positives = 38/48 (79%)
Frame = +3 / -3
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVV 701
G VVSGSSMATTAPK V+++L DA CA+R AVG PA TSWKYT +V
Sbjct: 267 GLVVSGSSMATTAPKKVEELLVDAACAIRIAVGLFPARTSWKYTLNLV 124
Score = 78.0 bits (164), Expect(2) = 1e-060
Identities = 32/49 (65%), Positives = 36/49 (73%)
Frame = -2 / +2
Query: 703 LTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
LT +VYFHDV+AG +PTA+RIAQ A FGAVVAIDDPLTT P
Sbjct: 122 LTKFKVYFHDVLAGKSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTSP 268
>gb|BM371288.2|BM371288 EBma08_SQ002_C11_R maternal, 28 DPA, no treatment, cv Optic, EBma08
Hordeum vulgare subsp. vulgare cDNA clone
EBma08_SQ002_C11 5', mRNA sequence
Length = 601
Score = 167 bits (360), Expect(2) = 1e-059
Identities = 71/88 (80%), Positives = 79/88 (89%)
Frame = -2 / +3
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
E+GRAQGTYTFADQ TFGLLM MNFVFTAG++ GS L+I GRNEVLS VREMSIVGG+GK
Sbjct: 333 EIGRAQGTYTFADQATFGLLMNMNFVFTAGDYKGSGLTIYGRNEVLSAVREMSIVGGTGK 512
Query: 358 FRMARGYVQAHTIDSGATSGTTVVQYTV 275
FRMARGYV+A T+DSGA SG TVV+YTV
Sbjct: 513 FRMARGYVEARTMDSGAKSGETVVEYTV 596
Score = 128 bits (274), Expect(2) = 5e-047
Identities = 60/90 (66%), Positives = 63/90 (70%)
Frame = +3 / -1
Query: 270 TLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPLCS 449
T TVY TTV P++APESMV A T PRAMRNLP+PPTMLI P M LPL S
Sbjct: 601 T*TVYSTTVSPDLAPESMVRAST*PRAMRNLPVPPTMLISLTADSTSLRP*MVRPLPL*S 422
Query: 450 PAVNTKFMTMRRPNVCWSANV*VPWARPTS 539
PAV TKFM MRRP V WSANV*VP ARP S
Sbjct: 421 PAVKTKFMFMRRPKVAWSANV*VPCARPIS 332
Score = 82.6 bits (174), Expect(2) = 1e-059
Identities = 33/50 (66%), Positives = 39/50 (78%)
Frame = -2 / +3
Query: 706 GLTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
GLT ++Y+HDV+AGT+PTA+RIAQ A FGAVVAIDDPLTTGP
Sbjct: 153 GLTKFKLYWHDVLAGTSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTGP 302
Score = 79.4 bits (167), Expect(2) = 5e-047
Identities = 33/50 (66%), Positives = 41/50 (82%)
Frame = +3 / -1
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVVNP 707
GPVVSGSSMATTAPK V+++L DA CA+R AVG VPA+TS +Y+ +V P
Sbjct: 301 GPVVSGSSMATTAPKKVEELLVDAACAIRIAVGLVPASTSCQYSLNLVRP 152
>gb|BQ763387.1|BQ763387 EBro02_SQ008_O04_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ008_O04 5', mRNA sequence
Length = 543
Score = 167 bits (360), Expect(2) = 1e-059
Identities = 71/88 (80%), Positives = 79/88 (89%)
Frame = -2 / +2
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
E+GRAQGTYTFADQ TFGLLM MNFVFTAG++ GS L+I GRNEVLS VREMSIVGG+GK
Sbjct: 275 EIGRAQGTYTFADQATFGLLMNMNFVFTAGDYKGSGLTIYGRNEVLSAVREMSIVGGTGK 454
Query: 358 FRMARGYVQAHTIDSGATSGTTVVQYTV 275
FRMARGYV+A T+DSGA SG TVV+YTV
Sbjct: 455 FRMARGYVEARTMDSGAKSGETVVEYTV 538
Score = 128 bits (274), Expect(2) = 5e-047
Identities = 60/90 (66%), Positives = 63/90 (70%)
Frame = +3 / -1
Query: 270 TLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPLCS 449
T TVY TTV P++APESMV A T PRAMRNLP+PPTMLI P M LPL S
Sbjct: 543 T*TVYSTTVSPDLAPESMVRAST*PRAMRNLPVPPTMLISLTADSTSLRP*MVRPLPL*S 364
Query: 450 PAVNTKFMTMRRPNVCWSANV*VPWARPTS 539
PAV TKFM MRRP V WSANV*VP ARP S
Sbjct: 363 PAVKTKFMFMRRPKVAWSANV*VPCARPIS 274
Score = 82.6 bits (174), Expect(2) = 1e-059
Identities = 33/50 (66%), Positives = 39/50 (78%)
Frame = -2 / +2
Query: 706 GLTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
GLT ++Y+HDV+AGT+PTA+RIAQ A FGAVVAIDDPLTTGP
Sbjct: 95 GLTKFKLYWHDVLAGTSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTGP 244
Score = 79.4 bits (167), Expect(2) = 5e-047
Identities = 33/50 (66%), Positives = 41/50 (82%)
Frame = +3 / -1
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVVNP 707
GPVVSGSSMATTAPK V+++L DA CA+R AVG VPA+TS +Y+ +V P
Sbjct: 243 GPVVSGSSMATTAPKKVEELLVDAACAIRIAVGLVPASTSCQYSLNLVRP 94
>gb|BE193614.2|BE193614 HVSMEh0081O22f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0081O22f, mRNA sequence
Length = 756
Score = 167 bits (359), Expect(2) = 1e-059
Identities = 72/91 (79%), Positives = 80/91 (87%)
Frame = -2 / +2
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
E+GRAQGTYTFADQ TFGLLM MNFVFTAG++ GS L+I GRNEVLS VREMSIVGG+GK
Sbjct: 212 EIGRAQGTYTFADQATFGLLMNMNFVFTAGDYKGSGLTIYGRNEVLSAVREMSIVGGTGK 391
Query: 358 FRMARGYVQAHTIDSGATSGTTVVQYTVNVK 266
FRMARGYV+A T+DS A SG TVV+YTV VK
Sbjct: 392 FRMARGYVEARTMDSRAKSGETVVEYTVYVK 484
Score = 126 bits (269), Expect(2) = 3e-046
Identities = 60/91 (65%), Positives = 63/91 (69%)
Frame = +3 / -1
Query: 267 LTLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPLC 446
LT TVY TTV P++A ESMV A T PRAMRNLP+PPTMLI P M LPL
Sbjct: 483 LT*TVYSTTVSPDLARESMVRAST*PRAMRNLPVPPTMLISLTADSTSLRP*MVRPLPL* 304
Query: 447 SPAVNTKFMTMRRPNVCWSANV*VPWARPTS 539
SPAV TKFM MRRP V WSANV*VP ARP S
Sbjct: 303 SPAVKTKFMFMRRPKVAWSANV*VPCARPIS 211
Score = 82.6 bits (174), Expect(2) = 1e-059
Identities = 33/50 (66%), Positives = 39/50 (78%)
Frame = -2 / +2
Query: 706 GLTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
GLT ++Y+HDV+AGT+PTA+RIAQ A FGAVVAIDDPLTTGP
Sbjct: 32 GLTKFKLYWHDVLAGTSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTGP 181
Score = 79.4 bits (167), Expect(2) = 3e-046
Identities = 33/50 (66%), Positives = 41/50 (82%)
Frame = +3 / -1
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVVNP 707
GPVVSGSSMATTAPK V+++L DA CA+R AVG VPA+TS +Y+ +V P
Sbjct: 180 GPVVSGSSMATTAPKKVEELLVDAACAIRIAVGLVPASTSCQYSLNLVRP 31
>gb|BI957678.1|BI957678 HVSMEn0010J22f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0010J22f, mRNA sequence
Length = 659
Score = 162 bits (348), Expect(2) = 4e-058
Identities = 71/92 (77%), Positives = 79/92 (85%)
Frame = -2 / +1
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
E+GRAQGTYTFADQ TFGLLM MNFVFTAG++ GS L+I GRNEVLS VREMSIV G+GK
Sbjct: 286 EIGRAQGTYTFADQATFGLLMNMNFVFTAGDYKGSGLTIYGRNEVLSAVREMSIVWGTGK 465
Query: 358 FRMARGYVQAHTIDSGATSGTTVVQYTVNVKA 263
FRMARGYV+A T+DS A SG T V+YTV VKA
Sbjct: 466 FRMARGYVEARTMDSRAKSGETGVEYTVYVKA 561
Score = 121 bits (259), Expect(2) = 6e-045
Identities = 59/92 (64%), Positives = 62/92 (67%)
Frame = +3 / -1
Query: 264 ALTLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPL 443
ALT TVY T V P++A ESMV A T PRAMRNLP+P TMLI P M LPL
Sbjct: 560 ALT*TVYSTPVSPDLARESMVRAST*PRAMRNLPVPQTMLISLTADSTSLRP*MVRPLPL 381
Query: 444 CSPAVNTKFMTMRRPNVCWSANV*VPWARPTS 539
SPAV TKFM MRRP V WSANV*VP ARP S
Sbjct: 380 *SPAVKTKFMFMRRPKVAWSANV*VPCARPIS 285
Score = 82.6 bits (174), Expect(2) = 4e-058
Identities = 33/50 (66%), Positives = 39/50 (78%)
Frame = -2 / +1
Query: 706 GLTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
GLT ++Y+HDV+AGT+PTA+RIAQ A FGAVVAIDDPLTTGP
Sbjct: 106 GLTKFKLYWHDVLAGTSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTGP 255
Score = 79.4 bits (167), Expect(2) = 6e-045
Identities = 33/50 (66%), Positives = 41/50 (82%)
Frame = +3 / -1
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVVNP 707
GPVVSGSSMATTAPK V+++L DA CA+R AVG VPA+TS +Y+ +V P
Sbjct: 254 GPVVSGSSMATTAPKKVEELLVDAACAIRIAVGLVPASTSCQYSLNLVRP 105
>gb|BU979623.1|BU979623 HA16L23r HA Hordeum vulgare subsp. vulgare cDNA clone HA16L23
5-PRIME, mRNA sequence
Length = 540
Score = 160 bits (344), Expect(2) = 1e-057
Identities = 68/85 (80%), Positives = 76/85 (89%)
Frame = -2 / +3
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
E+GRAQGTYTFADQ TFGLLM MNFVFTAG++ GS L+I GRNEVLS VREMSIVGG+GK
Sbjct: 285 EIGRAQGTYTFADQATFGLLMNMNFVFTAGDYKGSGLTIYGRNEVLSAVREMSIVGGTGK 464
Query: 358 FRMARGYVQAHTIDSGATSGTTVVQ 284
FRMARGYV+A T+DSGA SG TVV+
Sbjct: 465 FRMARGYVEARTMDSGAKSGETVVE 539
Score = 122 bits (260), Expect(2) = 4e-045
Identities = 56/84 (66%), Positives = 59/84 (70%)
Frame = +3 / -3
Query: 288 TTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPLCSPAVNTK 467
TTV P++APESMV A T PRAMRNLP+PPTMLI P M LPL SPAV TK
Sbjct: 535 TTVSPDLAPESMVRAST*PRAMRNLPVPPTMLISLTADSTSLRP*MVRPLPL*SPAVKTK 356
Query: 468 FMTMRRPNVCWSANV*VPWARPTS 539
FM MRRP V WSANV*VP ARP S
Sbjct: 355 FMFMRRPKVAWSANV*VPCARPIS 284
Score = 82.6 bits (174), Expect(2) = 1e-057
Identities = 33/50 (66%), Positives = 39/50 (78%)
Frame = -2 / +3
Query: 706 GLTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
GLT ++Y+HDV+AGT+PTA+RIAQ A FGAVVAIDDPLTTGP
Sbjct: 105 GLTKFKLYWHDVLAGTSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTGP 254
Score = 79.4 bits (167), Expect(2) = 4e-045
Identities = 33/50 (66%), Positives = 41/50 (82%)
Frame = +3 / -3
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVVNP 707
GPVVSGSSMATTAPK V+++L DA CA+R AVG VPA+TS +Y+ +V P
Sbjct: 253 GPVVSGSSMATTAPKKVEELLVDAACAIRIAVGLVPASTSCQYSLNLVRP 104
>gb|BU981234.1|BU981234 HA23A06r HA Hordeum vulgare subsp. vulgare cDNA clone HA23A06
5-PRIME, mRNA sequence
Length = 537
Score = 159 bits (342), Expect(2) = 3e-057
Identities = 68/84 (80%), Positives = 75/84 (89%)
Frame = -2 / +3
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
E+GRAQGTYTFADQ TFGLLM MNFVFTAG++ GS L+I GRNEVLS VREMSIVGG+GK
Sbjct: 285 EIGRAQGTYTFADQATFGLLMNMNFVFTAGDYKGSGLTIYGRNEVLSAVREMSIVGGTGK 464
Query: 358 FRMARGYVQAHTIDSGATSGTTVV 287
FRMARGYV+A T+DSGA SG TVV
Sbjct: 465 FRMARGYVEARTMDSGAKSGETVV 536
Score = 122 bits (260), Expect(2) = 4e-045
Identities = 56/84 (66%), Positives = 59/84 (70%)
Frame = +3 / -3
Query: 288 TTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPLCSPAVNTK 467
TTV P++APESMV A T PRAMRNLP+PPTMLI P M LPL SPAV TK
Sbjct: 535 TTVSPDLAPESMVRAST*PRAMRNLPVPPTMLISLTADSTSLRP*MVRPLPL*SPAVKTK 356
Query: 468 FMTMRRPNVCWSANV*VPWARPTS 539
FM MRRP V WSANV*VP ARP S
Sbjct: 355 FMFMRRPKVAWSANV*VPCARPIS 284
Score = 82.6 bits (174), Expect(2) = 3e-057
Identities = 33/50 (66%), Positives = 39/50 (78%)
Frame = -2 / +3
Query: 706 GLTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
GLT ++Y+HDV+AGT+PTA+RIAQ A FGAVVAIDDPLTTGP
Sbjct: 105 GLTKFKLYWHDVLAGTSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTGP 254
Score = 79.4 bits (167), Expect(2) = 4e-045
Identities = 33/50 (66%), Positives = 41/50 (82%)
Frame = +3 / -3
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVVNP 707
GPVVSGSSMATTAPK V+++L DA CA+R AVG VPA+TS +Y+ +V P
Sbjct: 253 GPVVSGSSMATTAPKKVEELLVDAACAIRIAVGLVPASTSCQYSLNLVRP 104
>gb|BQ459061.1|BQ459061 HA02H05r HA Hordeum vulgare subsp. vulgare cDNA clone HA02H05
5-PRIME, mRNA sequence
Length = 546
Score = 163 bits (351), Expect(2) = 4e-057
Identities = 70/84 (83%), Positives = 76/84 (90%)
Frame = -2 / +3
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
EVGRAQGTYTFADQ TFGLLM MNFVFTAGE+ GS+L+I GRNEVLS VREMSI+GG+GK
Sbjct: 294 EVGRAQGTYTFADQATFGLLMNMNFVFTAGEYKGSSLTIYGRNEVLSAVREMSILGGTGK 473
Query: 358 FRMARGYVQAHTIDSGATSGTTVV 287
FRMARGYVQA T+DSGA SG TVV
Sbjct: 474 FRMARGYVQASTVDSGANSGETVV 545
Score = 123 bits (263), Expect(2) = 4e-045
Identities = 56/84 (66%), Positives = 59/84 (70%)
Frame = +3 / -3
Query: 288 TTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPLCSPAVNTK 467
TTV PE+APES V A T PRAMRNLP+PP MLI P M +LPL SPAVNTK
Sbjct: 544 TTVSPELAPESTVLAWT*PRAMRNLPVPPRMLISLTAESTSLRP*MVRLLPLYSPAVNTK 365
Query: 468 FMTMRRPNVCWSANV*VPWARPTS 539
FM MR P V WSANV*VP ARPTS
Sbjct: 364 FMFMRSPKVAWSANV*VPCARPTS 293
Score = 78.0 bits (164), Expect(2) = 4e-045
Identities = 33/48 (68%), Positives = 38/48 (79%)
Frame = +3 / -3
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVV 701
G VVSGSSMATTAPK V+++L DA CA+R AVG PA TSWKYT +V
Sbjct: 262 GLVVSGSSMATTAPKKVEELLVDAACAIRIAVGLFPARTSWKYTLNLV 119
Score = 78.0 bits (164), Expect(2) = 4e-057
Identities = 32/49 (65%), Positives = 36/49 (73%)
Frame = -2 / +3
Query: 703 LTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
LT +VYFHDV+AG +PTA+RIAQ A FGAVVAIDDPLTT P
Sbjct: 117 LTKFKVYFHDVLAGKSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTSP 263
>gb|BI960172.1|BI960172 HVSMEn0023I14f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0023I14f, mRNA sequence
Length = 644
Score = 112 bits (240), Expect(3) = 6e-057
Identities = 48/58 (82%), Positives = 53/58 (91%)
Frame = -2 / +2
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGS 365
EVGRAQGTYTFADQ TFGLLM MNFVFTAGE+ GS+L+I GRNEVLS VREMSI+GG+
Sbjct: 287 EVGRAQGTYTFADQATFGLLMNMNFVFTAGEYKGSSLTIYGRNEVLSAVREMSILGGT 460
Score = 80.8 bits (170), Expect(3) = 1e-044
Identities = 36/58 (62%), Positives = 38/58 (65%)
Frame = +3 / -3
Query: 366 LPPTMLIXXXXXXXXXXPRMESVLPLCSPAVNTKFMTMRRPNVCWSANV*VPWARPTS 539
+PP MLI P M +LPL SPAVNTKFM MR P V WSANV*VP ARPTS
Sbjct: 459 VPPRMLISLTAESTSLRP*MVRLLPLYSPAVNTKFMFMRSPKVAWSANV*VPCARPTS 286
Score = 78.0 bits (164), Expect(3) = 1e-044
Identities = 33/48 (68%), Positives = 38/48 (79%)
Frame = +3 / -3
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVV 701
G VVSGSSMATTAPK V+++L DA CA+R AVG PA TSWKYT +V
Sbjct: 255 GLVVSGSSMATTAPKKVEELLVDAACAIRIAVGLFPARTSWKYTLNLV 112
Score = 78.0 bits (164), Expect(3) = 6e-057
Identities = 32/49 (65%), Positives = 36/49 (73%)
Frame = -2 / +2
Query: 703 LTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
LT +VYFHDV+AG +PTA+RIAQ A FGAVVAIDDPLTT P
Sbjct: 110 LTKFKVYFHDVLAGKSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTSP 256
Score = 68.9 bits (144), Expect(3) = 6e-057
Identities = 29/37 (78%), Positives = 31/37 (83%)
Frame = -2 / +2
Query: 373 GGSGKFRMARGYVQAHTIDSGATSGTTVVQYTVNVKA 263
G GKFRMARGYVQA T+DSGA SG TVV+YTV VKA
Sbjct: 455 GTXGKFRMARGYVQASTVDSGANSGETVVEYTVYVKA 565
Score = 59.7 bits (124), Expect(3) = 1e-044
Identities = 27/41 (65%), Positives = 30/41 (73%)
Frame = +3 / -3
Query: 264 ALTLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLI 386
ALT TVY TTV PE+APES V A T PRAMRNLP P ++
Sbjct: 564 ALTYTVYSTTVSPELAPESTVLAWT*PRAMRNLPXVPPRML 442
>gb|BG417730.2|BG417730 HVSMEk0019E17f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0019E17f, mRNA sequence
Length = 877
Score = 161 bits (347), Expect(2) = 1e-056
Identities = 72/92 (78%), Positives = 78/92 (84%)
Frame = -2 / +3
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
EVGRAQGTYTFADQ TFGLLM MNFVFTAGE+ GS+L+I RNEVLS VREMSI+GG+GK
Sbjct: 279 EVGRAQGTYTFADQATFGLLMNMNFVFTAGEYKGSSLTIYCRNEVLSAVREMSILGGTGK 458
Query: 358 FRMARGYVQAHTIDSGATSGTTVVQYTVNVKA 263
FRMARGY+QA T DSGA S TVV YTV V A
Sbjct: 459 FRMARGYLQASTGDSGANSSKTVVXYTVYVNA 554
Score = 125 bits (267), Expect(2) = 1e-045
Identities = 59/92 (64%), Positives = 63/92 (68%)
Frame = +3 / -1
Query: 264 ALTLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPL 443
ALT TVY TTV+ E+APES V A PRAMRNLP+PP MLI M +LPL
Sbjct: 553 ALTYTVYXTTVLLELAPESPVLAWR*PRAMRNLPVPPRMLISLTAESTSLRQ*MVRLLPL 374
Query: 444 CSPAVNTKFMTMRRPNVCWSANV*VPWARPTS 539
SPAVNTKFM MR P V WSANV*VP ARPTS
Sbjct: 373 YSPAVNTKFMFMRSPKVAWSANV*VPCARPTS 278
Score = 78.0 bits (164), Expect(2) = 1e-045
Identities = 33/48 (68%), Positives = 38/48 (79%)
Frame = +3 / -1
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVV 701
G VVSGSSMATTAPK V+++L DA CA+R AVG PA TSWKYT +V
Sbjct: 247 GLVVSGSSMATTAPKKVEELLVDAACAIRIAVGLFPARTSWKYTLNLV 104
Score = 78.0 bits (164), Expect(2) = 1e-056
Identities = 32/49 (65%), Positives = 36/49 (73%)
Frame = -2 / +3
Query: 703 LTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
LT +VYFHDV+AG +PTA+RIAQ A FGAVVAIDDPLTT P
Sbjct: 102 LTKFKVYFHDVLAGKSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTSP 248
>gb|BG343533.2|BG343533 HVSMEg0006F02f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0006F02f, mRNA sequence
Length = 962
Score = 153 bits (329), Expect(2) = 4e-054
Identities = 65/78 (83%), Positives = 71/78 (91%)
Frame = -2 / +2
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
EVGRAQGTYTFADQ TFGLLM MNFVFTAGE+ GS+L+I GRNEVLS VREMSI+GG+GK
Sbjct: 284 EVGRAQGTYTFADQATFGLLMNMNFVFTAGEYKGSSLTIYGRNEVLSAVREMSILGGTGK 463
Query: 358 FRMARGYVQAHTIDSGAT 305
FRMARGYVQA T+DSG T
Sbjct: 464 FRMARGYVQASTVDSGPT 517
Score = 111 bits (238), Expect(2) = 1e-041
Identities = 51/78 (65%), Positives = 53/78 (67%)
Frame = +3 / -3
Query: 306 VAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPLCSPAVNTKFMTMRR 485
V PES V A T PRAMRNLP+PP MLI P M +LPL SPAVNTKFM MR
Sbjct: 516 VGPESTVLAWT*PRAMRNLPVPPRMLISLTAESTSLRP*MVRLLPLYSPAVNTKFMFMRS 337
Query: 486 PNVCWSANV*VPWARPTS 539
P V WSANV*VP ARPTS
Sbjct: 336 PKVAWSANV*VPCARPTS 283
Score = 78.0 bits (164), Expect(2) = 1e-041
Identities = 33/48 (68%), Positives = 38/48 (79%)
Frame = +3 / -3
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVV 701
G VVSGSSMATTAPK V+++L DA CA+R AVG PA TSWKYT +V
Sbjct: 252 GLVVSGSSMATTAPKKVEELLVDAACAIRIAVGLFPARTSWKYTLNLV 109
Score = 78.0 bits (164), Expect(2) = 4e-054
Identities = 32/49 (65%), Positives = 36/49 (73%)
Frame = -2 / +2
Query: 703 LTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
LT +VYFHDV+AG +PTA+RIAQ A FGAVVAIDDPLTT P
Sbjct: 107 LTKFKVYFHDVLAGKSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTSP 253
>gb|BU982763.1|BU982763 HA27I15r HA Hordeum vulgare subsp. vulgare cDNA clone HA27I15
5-PRIME, mRNA sequence
Length = 587
Score = 148 bits (318), Expect(2) = 5e-054
Identities = 62/77 (80%), Positives = 70/77 (90%)
Frame = -2 / +2
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
E+GRAQGTYTFADQ TFGLLM MNFVFTAG++ GS L+I GRNEVLS VR+MSIVGG+GK
Sbjct: 353 EIGRAQGTYTFADQATFGLLMNMNFVFTAGDYKGSGLTIYGRNEVLSAVRDMSIVGGTGK 532
Query: 358 FRMARGYVQAHTIDSGA 308
FRMARGYV+A T+DSGA
Sbjct: 533 FRMARGYVEARTMDSGA 583
Score = 112 bits (239), Expect(2) = 3e-042
Identities = 52/78 (66%), Positives = 54/78 (69%)
Frame = +3 / -3
Query: 306 VAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPLCSPAVNTKFMTMRR 485
+APESMV A T PRAMRNLP+PPTMLI P M LPL SPAV TKFM MRR
Sbjct: 585 LAPESMVRAST*PRAMRNLPVPPTMLISLTADSTSLRP*MVRPLPL*SPAVKTKFMFMRR 406
Query: 486 PNVCWSANV*VPWARPTS 539
P V WSANV*VP ARP S
Sbjct: 405 PKVAWSANV*VPCARPIS 352
Score = 82.6 bits (174), Expect(2) = 5e-054
Identities = 33/50 (66%), Positives = 39/50 (78%)
Frame = -2 / +2
Query: 706 GLTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
GLT ++Y+HDV+AGT+PTA+RIAQ A FGAVVAIDDPLTTGP
Sbjct: 173 GLTKFKLYWHDVLAGTSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTGP 322
Score = 79.4 bits (167), Expect(2) = 3e-042
Identities = 33/50 (66%), Positives = 41/50 (82%)
Frame = +3 / -3
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVVNP 707
GPVVSGSSMATTAPK V+++L DA CA+R AVG VPA+TS +Y+ +V P
Sbjct: 321 GPVVSGSSMATTAPKKVEELLVDAACAIRIAVGLVPASTSCQYSLNLVRP 172
>gb|AV916732.1|AV916732 AV916732 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19h04 5', mRNA sequence
Length = 566
Score = 177 bits (380), Expect(2) = 3e-051
Identities = 76/92 (82%), Positives = 83/92 (90%)
Frame = -2 / +1
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
EVGRAQGTYTFADQ TFGLLM MNFVFTAGE+ GS+L+I GRNEVLS VREMSI+GG+GK
Sbjct: 130 EVGRAQGTYTFADQATFGLLMNMNFVFTAGEYKGSSLTIYGRNEVLSAVREMSILGGTGK 309
Query: 358 FRMARGYVQAHTIDSGATSGTTVVQYTVNVKA 263
FRMARGYVQA T+DSGA SG TVV+YTV VKA
Sbjct: 310 FRMARGYVQASTVDSGANSGETVVEYTVYVKA 405
Score = 134 bits (288), Expect(2) = 5e-040
Identities = 62/92 (67%), Positives = 65/92 (70%)
Frame = +3 / -1
Query: 264 ALTLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPL 443
ALT TVY TTV PE+APES V A T PRAMRNLP+PP MLI P M +LPL
Sbjct: 404 ALTYTVYSTTVSPELAPESTVLAWT*PRAMRNLPVPPRMLISLTAESTSLRP*MVRLLPL 225
Query: 444 CSPAVNTKFMTMRRPNVCWSANV*VPWARPTS 539
SPAVNTKFM MR P V WSANV*VP ARPTS
Sbjct: 224 YSPAVNTKFMFMRSPKVAWSANV*VPCARPTS 129
Score = 49.6 bits (102), Expect(2) = 5e-040
Identities = 21/29 (72%), Positives = 25/29 (86%)
Frame = +3 / -1
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMR 644
G VVSGSSMATTAPK V+++L DA CA+R
Sbjct: 98 GLVVSGSSMATTAPKKVEELLVDAACAIR 12
Score = 45.1 bits (92), Expect(2) = 3e-051
Identities = 19/29 (65%), Positives = 19/29 (65%)
Frame = -2 / +1
Query: 643 RIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
RIAQ A FGAVVAIDDPLTT P
Sbjct: 13 RIAQAASTNSSSTFFGAVVAIDDPLTTSP 99
>gb|AV922026.1|AV922026 AV922026 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19h04 3', mRNA sequence
Length = 519
Score = 177 bits (380), Expect(2) = 3e-051
Identities = 76/92 (82%), Positives = 83/92 (90%)
Frame = -2 / -3
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
EVGRAQGTYTFADQ TFGLLM MNFVFTAGE+ GS+L+I GRNEVLS VREMSI+GG+GK
Sbjct: 400 EVGRAQGTYTFADQATFGLLMNMNFVFTAGEYKGSSLTIYGRNEVLSAVREMSILGGTGK 221
Query: 358 FRMARGYVQAHTIDSGATSGTTVVQYTVNVKA 263
FRMARGYVQA T+DSGA SG TVV+YTV VKA
Sbjct: 220 FRMARGYVQASTVDSGANSGETVVEYTVYVKA 125
Score = 134 bits (288), Expect(2) = 5e-040
Identities = 62/92 (67%), Positives = 65/92 (70%)
Frame = +3 / +3
Query: 264 ALTLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPL 443
ALT TVY TTV PE+APES V A T PRAMRNLP+PP MLI P M +LPL
Sbjct: 126 ALTYTVYSTTVSPELAPESTVLAWT*PRAMRNLPVPPRMLISLTAESTSLRP*MVRLLPL 305
Query: 444 CSPAVNTKFMTMRRPNVCWSANV*VPWARPTS 539
SPAVNTKFM MR P V WSANV*VP ARPTS
Sbjct: 306 YSPAVNTKFMFMRSPKVAWSANV*VPCARPTS 401
Score = 49.6 bits (102), Expect(2) = 5e-040
Identities = 21/29 (72%), Positives = 25/29 (86%)
Frame = +3 / +3
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMR 644
G VVSGSSMATTAPK V+++L DA CA+R
Sbjct: 432 GLVVSGSSMATTAPKKVEELLVDAACAIR 518
Score = 45.1 bits (92), Expect(2) = 3e-051
Identities = 19/29 (65%), Positives = 19/29 (65%)
Frame = -2 / -3
Query: 643 RIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
RIAQ A FGAVVAIDDPLTT P
Sbjct: 517 RIAQAASTNSSSTFFGAVVAIDDPLTTSP 431
>gb|BF625296.2|BF625296 HVSMEa0008E19f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0008E19f, mRNA sequence
Length = 706
Score = 112 bits (239), Expect(4) = 5e-051
Identities = 48/60 (80%), Positives = 53/60 (88%)
Frame = -2 / +3
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
EVGRAQGTYTFADQ TFGLLM MNFVFTAGE+ GS L+I GRNEVLS VREM I+GG+G+
Sbjct: 273 EVGRAQGTYTFADQATFGLLMNMNFVFTAGEYKGSILTIYGRNEVLSAVREMIILGGTGQ 452
Score = 83.1 bits (175), Expect(4) = 3e-039
Identities = 36/59 (61%), Positives = 39/59 (66%)
Frame = +3 / -1
Query: 363 PLPPTMLIXXXXXXXXXXPRMESVLPLCSPAVNTKFMTMRRPNVCWSANV*VPWARPTS 539
P+PP M+I P M +LPL SPAVNTKFM MR P V WSANV*VP ARPTS
Sbjct: 448 PVPPRMIISLTAESTSFRP*MVRMLPLYSPAVNTKFMFMRSPKVAWSANV*VPCARPTS 272
Score = 78.0 bits (164), Expect(4) = 3e-039
Identities = 33/48 (68%), Positives = 38/48 (79%)
Frame = +3 / -1
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVV 701
G VVSGSSMATTAPK V+++L DA CA+R AVG PA TSWKYT +V
Sbjct: 241 GLVVSGSSMATTAPKKVEELLVDAACAIRIAVGLFPARTSWKYTLNLV 98
Score = 78.0 bits (164), Expect(4) = 5e-051
Identities = 32/49 (65%), Positives = 36/49 (73%)
Frame = -2 / +3
Query: 703 LTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
LT +VYFHDV+AG +PTA+RIAQ A FGAVVAIDDPLTT P
Sbjct: 96 LTKFKVYFHDVLAGKSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTSP 242
Score = 38.6 bits (78), Expect(4) = 5e-051
Identities = 15/21 (71%), Positives = 18/21 (85%)
Frame = -2 / +2
Query: 325 TIDSGATSGTTVVQYTVNVKA 263
T+DSGA SG TV++YTV VKA
Sbjct: 488 TVDSGANSGETVIEYTVYVKA 550
Score = 33.6 bits (67), Expect(4) = 3e-039
Identities = 15/21 (71%), Positives = 16/21 (76%)
Frame = +3 / -2
Query: 264 ALTLTVYWTTVVPEVAPESMV 326
ALT TVY TV PE+APES V
Sbjct: 549 ALTYTVYSMTVSPELAPESTV 487
Score = 28.6 bits (56), Expect(4) = 5e-051
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = -2 / +1
Query: 373 GGSGKFRMARGYVQA 329
G KFRMA GYVQA
Sbjct: 439 GARAKFRMAHGYVQA 483
Score = 23.1 bits (44), Expect(4) = 3e-039
Identities = 10/15 (66%), Positives = 10/15 (66%)
Frame = +3 / -3
Query: 330 ACTYPRAMRNLPLPP 374
ACT P AMRNL P
Sbjct: 482 ACT*P*AMRNLARAP 438
>gb|BQ664296.1|BQ664296 HV02L22u HV Hordeum vulgare subsp. vulgare cDNA clone HV02L22
3-PRIME, mRNA sequence
Length = 510
Score = 177 bits (380), Expect(2) = 1e-050
Identities = 76/92 (82%), Positives = 83/92 (90%)
Frame = -2 / -2
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
EVGRAQGTYTFADQ TFGLLM MNFVFTAGE+ GS+L+I GRNEVLS VREMSI+GG+GK
Sbjct: 395 EVGRAQGTYTFADQATFGLLMNMNFVFTAGEYKGSSLTIYGRNEVLSAVREMSILGGTGK 216
Query: 358 FRMARGYVQAHTIDSGATSGTTVVQYTVNVKA 263
FRMARGYVQA T+DSGA SG TVV+YTV VKA
Sbjct: 215 FRMARGYVQASTVDSGANSGETVVEYTVYVKA 120
Score = 134 bits (288), Expect(2) = 2e-039
Identities = 62/92 (67%), Positives = 65/92 (70%)
Frame = +3 / +1
Query: 264 ALTLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPL 443
ALT TVY TTV PE+APES V A T PRAMRNLP+PP MLI P M +LPL
Sbjct: 121 ALTYTVYSTTVSPELAPESTVLAWT*PRAMRNLPVPPRMLISLTAESTSLRP*MVRLLPL 300
Query: 444 CSPAVNTKFMTMRRPNVCWSANV*VPWARPTS 539
SPAVNTKFM MR P V WSANV*VP ARPTS
Sbjct: 301 YSPAVNTKFMFMRSPKVAWSANV*VPCARPTS 396
Score = 47.3 bits (97), Expect(2) = 2e-039
Identities = 20/28 (71%), Positives = 24/28 (85%)
Frame = +3 / +1
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAM 641
G VVSGSSMATTAPK V+++L DA CA+
Sbjct: 427 GLVVSGSSMATTAPKKVEELLVDAACAI 510
Score = 42.8 bits (87), Expect(2) = 1e-050
Identities = 18/28 (64%), Positives = 18/28 (64%)
Frame = -2 / -2
Query: 640 IAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
IAQ A FGAVVAIDDPLTT P
Sbjct: 509 IAQAASTNSSSTFFGAVVAIDDPLTTSP 426
>gb|BI960628.1|BI960628 HVSMEn0025G23f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0025G23f, mRNA sequence
Length = 645
Score = 125 bits (267), Expect(2) = 1e-045
Identities = 54/70 (77%), Positives = 60/70 (85%)
Frame = -2 / +1
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
EVGRAQGTYTFADQ TFGLLM MNFVFTAGE+ GS+L+I GRNEVLS VREMSI+GG+GK
Sbjct: 289 EVGRAQGTYTFADQATFGLLMNMNFVFTAGEYKGSSLTIYGRNEVLSAVREMSILGGTGK 468
Query: 358 FRMARGYVQA 329
F + YV A
Sbjct: 469 FPLDHRYVPA 498
Score = 88.6 bits (187), Expect(2) = 1e-034
Identities = 39/61 (63%), Positives = 41/61 (67%)
Frame = +3 / -2
Query: 357 NLPLPPTMLIXXXXXXXXXXPRMESVLPLCSPAVNTKFMTMRRPNVCWSANV*VPWARPT 536
NLP+PP MLI P M +LPL SPAVNTKFM MR P V WSANV*VP ARPT
Sbjct: 470 NLPVPPRMLISLTAESTSLRP*MVRLLPLYSPAVNTKFMFMRSPKVAWSANV*VPCARPT 291
Query: 537 S 539
S
Sbjct: 290 S 288
Score = 78.0 bits (164), Expect(2) = 1e-034
Identities = 33/48 (68%), Positives = 38/48 (79%)
Frame = +3 / -2
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVV 701
G VVSGSSMATTAPK V+++L DA CA+R AVG PA TSWKYT +V
Sbjct: 257 GLVVSGSSMATTAPKKVEELLVDAACAIRIAVGLFPARTSWKYTLNLV 114
Score = 78.0 bits (164), Expect(2) = 1e-045
Identities = 32/49 (65%), Positives = 36/49 (73%)
Frame = -2 / +1
Query: 703 LTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
LT +VYFHDV+AG +PTA+RIAQ A FGAVVAIDDPLTT P
Sbjct: 112 LTKFKVYFHDVLAGKSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTSP 258
>gb|BU982674.1|BU982674 HA27E09r HA Hordeum vulgare subsp. vulgare cDNA clone HA27E09
5-PRIME, mRNA sequence
Length = 532
Score = 116 bits (247), Expect(2) = 3e-044
Identities = 49/60 (81%), Positives = 54/60 (90%)
Frame = -2 / +1
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
E+GRAQGTYTFADQ TFGLLM MNFVFTAG++ GS L+I GRNEVLS VREMSIVGG+GK
Sbjct: 352 EIGRAQGTYTFADQATFGLLMNMNFVFTAGDYKGSGLTIYGRNEVLSAVREMSIVGGTGK 531
Score = 83.5 bits (176), Expect(2) = 1e-033
Identities = 38/60 (63%), Positives = 39/60 (65%)
Frame = +3 / -3
Query: 360 LPLPPTMLIXXXXXXXXXXPRMESVLPLCSPAVNTKFMTMRRPNVCWSANV*VPWARPTS 539
LP+PPTMLI P M LPL SPAV TKFM MRRP V WSANV*VP ARP S
Sbjct: 530 LPVPPTMLISLTADSTSLRP*MVRPLPL*SPAVKTKFMFMRRPKVAWSANV*VPCARPIS 351
Score = 82.6 bits (174), Expect(2) = 3e-044
Identities = 33/50 (66%), Positives = 39/50 (78%)
Frame = -2 / +1
Query: 706 GLTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
GLT ++Y+HDV+AGT+PTA+RIAQ A FGAVVAIDDPLTTGP
Sbjct: 172 GLTKFKLYWHDVLAGTSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTGP 321
Score = 79.4 bits (167), Expect(2) = 1e-033
Identities = 33/50 (66%), Positives = 41/50 (82%)
Frame = +3 / -3
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVVNP 707
GPVVSGSSMATTAPK V+++L DA CA+R AVG VPA+TS +Y+ +V P
Sbjct: 320 GPVVSGSSMATTAPKKVEELLVDAACAIRIAVGLVPASTSCQYSLNLVRP 171
>gb|AL510732.1|AL510732 AL510732 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY05N10u 3',
mRNA sequence
Length = 579
Score = 177 bits (380), Expect = 8e-044
Identities = 76/92 (82%), Positives = 83/92 (90%)
Frame = -2 / -1
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
EVGRAQGTYTFADQ TFGLLM MNFVFTAGE+ GS+L+I GRNEVLS VREMSI+GG+GK
Sbjct: 438 EVGRAQGTYTFADQATFGLLMNMNFVFTAGEYKGSSLTIYGRNEVLSAVREMSILGGTGK 259
Query: 358 FRMARGYVQAHTIDSGATSGTTVVQYTVNVKA 263
FRMARGYVQA T+DSGA SG TVV+YTV VKA
Sbjct: 258 FRMARGYVQASTVDSGANSGETVVEYTVYVKA 163
Score = 133 bits (285), Expect = 1e-030
Identities = 62/92 (67%), Positives = 65/92 (70%)
Frame = +3 / +2
Query: 264 ALTLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPL 443
ALT TVY TTV PE+APES V A T PRAMRNLP+PP MLI P M +LPL
Sbjct: 164 ALT*TVYSTTVSPELAPESTVLAWT*PRAMRNLPVPPRMLISLTAESTSLRP*MVRLLPL 343
Query: 444 CSPAVNTKFMTMRRPNVCWSANV*VPWARPTS 539
SPAVNTKFM MR P V WSANV*VP ARPTS
Sbjct: 344 YSPAVNTKFMFMRSPKVAWSANV*VPCARPTS 439
>gb|BG343276.2|BG343276 HVSMEg0005E17f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0005E17f, mRNA sequence
Length = 890
Score = 113 bits (241), Expect(2) = 4e-042
Identities = 50/66 (75%), Positives = 56/66 (84%)
Frame = -2 / +1
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
EVGRAQGTYTFADQ TFGLLM MNFVFTAGE+ GS+L+I GRNEVLS VREMSI G+G+
Sbjct: 292 EVGRAQGTYTFADQATFGLLMNMNFVFTAGEYKGSSLTIYGRNEVLSAVREMSIHRGTGQ 471
Query: 358 FRMARG 341
M+ G
Sbjct: 472 DAMSGG 489
Score = 81.7 bits (172), Expect(2) = 1e-032
Identities = 37/66 (56%), Positives = 41/66 (62%)
Frame = +3 / -1
Query: 342 PRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPLCSPAVNTKFMTMRRPNVCWSANV*VP 521
P + + P+P MLI P M +LPL SPAVNTKFM MR P V WSANV*VP
Sbjct: 488 PPLIASCPVPRCMLISLTAESTSLRP*MVRLLPLYSPAVNTKFMFMRSPKVAWSANV*VP 309
Query: 522 WARPTS 539
ARPTS
Sbjct: 308 CARPTS 291
Score = 78.0 bits (164), Expect(2) = 1e-032
Identities = 33/48 (68%), Positives = 38/48 (79%)
Frame = +3 / -1
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVV 701
G VVSGSSMATTAPK V+++L DA CA+R AVG PA TSWKYT +V
Sbjct: 260 GLVVSGSSMATTAPKKVEELLVDAACAIRIAVGLFPARTSWKYTLNLV 117
Score = 78.0 bits (164), Expect(2) = 4e-042
Identities = 32/49 (65%), Positives = 36/49 (73%)
Frame = -2 / +1
Query: 703 LTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
LT +VYFHDV+AG +PTA+RIAQ A FGAVVAIDDPLTT P
Sbjct: 115 LTKFKVYFHDVLAGKSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTSP 261
>gb|AL502849.1|AL502849 AL502849 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW08O16u 3', mRNA sequence
Length = 591
Score = 168 bits (361), Expect = 3e-041
Identities = 73/93 (78%), Positives = 80/93 (86%)
Frame = -2 / -3
Query: 541 TEVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSG 362
T AQGTYTFADQ TFGLLM MNFVFTAGE+ GS+L+I GRNEVLS VREMSI+GG+G
Sbjct: 565 TRWAAAQGTYTFADQATFGLLMNMNFVFTAGEYKGSSLTIYGRNEVLSAVREMSILGGTG 386
Query: 361 KFRMARGYVQAHTIDSGATSGTTVVQYTVNVKA 263
KFRMARGYVQA T+DSGA SG TVV+YTV VKA
Sbjct: 385 KFRMARGYVQASTVDSGANSGETVVEYTVYVKA 287
Score = 122 bits (262), Expect = 2e-027
Identities = 57/88 (64%), Positives = 60/88 (68%)
Frame = +3 / +3
Query: 264 ALTLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPL 443
ALT TVY TTV PE+APES V A T PRAMRNLP+PP MLI P M +LPL
Sbjct: 288 ALTYTVYSTTVSPELAPESTVLAWT*PRAMRNLPVPPRMLISLTAESTSLRP*MVRLLPL 467
Query: 444 CSPAVNTKFMTMRRPNVCWSANV*VPWA 527
SPAVNTKFM MR P V WSANV VP A
Sbjct: 468 YSPAVNTKFMFMRSPKVAWSANVXVPCA 551
>gb|BM444860.1|BM444860 EBem09_SQ006_L09_R embryo, 1 Day germination, no treatment, cv
Optic, EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ006_L09 5', mRNA sequence
Length = 327
Score = 167 bits (360), Expect = 5e-041
Identities = 72/88 (81%), Positives = 79/88 (89%)
Frame = -2 / +1
Query: 526 AQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGKFRMA 347
AQGTYTFADQ TFGLLM MNFVFTAGE+ GS+L+I GRNEVLS VREMSI+GG+GKFRMA
Sbjct: 1 AQGTYTFADQATFGLLMNMNFVFTAGEYKGSSLTIYGRNEVLSAVREMSILGGTGKFRMA 180
Query: 346 RGYVQAHTIDSGATSGTTVVQYTVNVKA 263
RGYVQA T+DSGA SG TVV+YTV VKA
Sbjct: 181 RGYVQASTVDSGANSGETVVEYTVYVKA 264
Score = 124 bits (265), Expect = 6e-028
Identities = 57/86 (66%), Positives = 60/86 (69%)
Frame = +3 / -2
Query: 264 ALTLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPL 443
ALT TVY TTV PE+APES V A T PRAMRNLP+PP MLI P M +LPL
Sbjct: 263 ALTYTVYSTTVSPELAPESTVLAWT*PRAMRNLPVPPRMLISLTAESTSLRP*MVRLLPL 84
Query: 444 CSPAVNTKFMTMRRPNVCWSANV*VP 521
SPAVNTKFM MR P V WSANV*VP
Sbjct: 83 YSPAVNTKFMFMRSPKVAWSANV*VP 6
>gb|CV056595.1|CV056595 BNEL19E4 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL19E4 5' similar to unknown
protein, mRNA sequence
Length = 359
Score = 167 bits (360), Expect = 5e-041
Identities = 72/88 (81%), Positives = 79/88 (89%)
Frame = -2 / +1
Query: 526 AQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGKFRMA 347
AQGTYTFADQ TFGLLM MNFVFTAGE+ GS+L+I GRNEVLS VREMSI+GG+GKFRMA
Sbjct: 1 AQGTYTFADQATFGLLMNMNFVFTAGEYKGSSLTIYGRNEVLSAVREMSILGGTGKFRMA 180
Query: 346 RGYVQAHTIDSGATSGTTVVQYTVNVKA 263
RGYVQA T+DSGA SG TVV+YTV VKA
Sbjct: 181 RGYVQASTVDSGANSGETVVEYTVYVKA 264
Score = 124 bits (265), Expect = 6e-028
Identities = 57/86 (66%), Positives = 60/86 (69%)
Frame = +3 / -1
Query: 264 ALTLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPL 443
ALT TVY TTV PE+APES V A T PRAMRNLP+PP MLI P M +LPL
Sbjct: 263 ALTYTVYSTTVSPELAPESTVLAWT*PRAMRNLPVPPRMLISLTAESTSLRP*MVRLLPL 84
Query: 444 CSPAVNTKFMTMRRPNVCWSANV*VP 521
SPAVNTKFM MR P V WSANV*VP
Sbjct: 83 YSPAVNTKFMFMRSPKVAWSANV*VP 6
>gb|BI958849.1|BI958849 HVSMEn0016O03f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0016O03f, mRNA sequence
Length = 668
Score = 165 bits (354), Expect = 3e-040
Identities = 71/92 (77%), Positives = 79/92 (85%)
Frame = -2 / -1
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGK 359
E+GRAQG YTFADQ T GLLM MN VFTAG++ GS L+I GRNEVLS VREMSIVGG+GK
Sbjct: 413 EIGRAQGPYTFADQATLGLLMNMNLVFTAGDYKGSGLTIYGRNEVLSPVREMSIVGGTGK 234
Query: 358 FRMARGYVQAHTIDSGATSGTTVVQYTVNVKA 263
FRMARGYV+A T+DSGA SG TVV+YTV VKA
Sbjct: 233 FRMARGYVEARTMDSGAKSGETVVEYTVYVKA 138
Score = 128 bits (274), Expect = 3e-029
Identities = 61/92 (66%), Positives = 64/92 (69%)
Frame = +3 / +1
Query: 264 ALTLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPL 443
ALT TVY TTV P++APESMV A T PRAMRNLP+PPTMLI P M LPL
Sbjct: 139 ALT*TVYSTTVSPDLAPESMVRAST*PRAMRNLPVPPTMLISLTGDSTSLRP*MVRPLPL 318
Query: 444 CSPAVNTKFMTMRRPNVCWSANV*VPWARPTS 539
SPAV TKFM MRRP V WSANV* P ARP S
Sbjct: 319 *SPAVKTKFMFMRRPKVAWSANV*GPCARPIS 414
>gb|CV054743.1|CV054743 BNEL112g9 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL112g9 5' similar to hypothetical
protein T10K17.300 - Arabidopsis thaliana, mRNA sequence
Length = 360
Score = 159 bits (341), Expect = 2e-038
Identities = 69/87 (79%), Positives = 76/87 (87%)
Frame = -2 / +1
Query: 523 QGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGKFRMAR 344
QGTYTFADQ TFGLLM MNFVFTAGE+ GS+L+I GRNEVLS VREMSI+GG+GK RMAR
Sbjct: 4 QGTYTFADQATFGLLMNMNFVFTAGEYKGSSLTIYGRNEVLSAVREMSILGGTGKVRMAR 183
Query: 343 GYVQAHTIDSGATSGTTVVQYTVNVKA 263
GY+QA T DSGA SG TVV+YTV VKA
Sbjct: 184 GYIQASTGDSGANSGETVVEYTVYVKA 264
Score = 115 bits (246), Expect = 2e-025
Identities = 54/86 (62%), Positives = 58/86 (67%)
Frame = +3 / -2
Query: 264 ALTLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPL 443
ALT TVY TTV PE+APES V A PRA+R LP+PP MLI P M +LPL
Sbjct: 263 ALTYTVYSTTVSPELAPESPVLA*M*PRAIRTLPVPPRMLISLTAESTSLRP*MVRLLPL 84
Query: 444 CSPAVNTKFMTMRRPNVCWSANV*VP 521
SPAVNTKFM MR P V WSANV*VP
Sbjct: 83 YSPAVNTKFMFMRSPKVAWSANV*VP 6
>gb|AJ433789.1|AJ433789 AJ433789 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200050C10F1, mRNA sequence
Length = 408
Score = 155 bits (332), Expect = 3e-037
Identities = 67/84 (79%), Positives = 74/84 (88%)
Frame = -2 / +1
Query: 514 YTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGKFRMARGYV 335
YTFADQ TFGLLM MNFVFTAG++ GS L+I GRNEVLS VREMSIVGG+GKFRMARGYV
Sbjct: 1 YTFADQATFGLLMNMNFVFTAGDYKGSGLTIYGRNEVLSAVREMSIVGGTGKFRMARGYV 180
Query: 334 QAHTIDSGATSGTTVVQYTVNVKA 263
+A T+DSGA SG TVV+YTV VKA
Sbjct: 181 EARTMDSGAKSGETVVEYTVYVKA 252
Score = 118 bits (253), Expect = 3e-026
Identities = 55/83 (66%), Positives = 58/83 (69%)
Frame = +3 / -2
Query: 264 ALTLTVYWTTVVPEVAPESMVCACTYPRAMRNLPLPPTMLIXXXXXXXXXXPRMESVLPL 443
ALT TVY TTV P++APESMV A T PRAMRNLP+PPTMLI P M LPL
Sbjct: 251 ALT*TVYSTTVSPDLAPESMVRAST*PRAMRNLPVPPTMLISLTADSTSLRP*MVRPLPL 72
Query: 444 CSPAVNTKFMTMRRPNVCWSANV 512
SPAV TKFM MRRP V WSANV
Sbjct: 71 *SPAVKTKFMFMRRPKVAWSANV 3
>gb|BQ760080.1|BQ760080 EBpi07_SQ003_K06_R pistil, 12 DPA, no treatment, cv Optic, EBpi07
Hordeum vulgare subsp. vulgare cDNA clone
EBpi07_SQ003_K06 5', mRNA sequence
Length = 462
Score = 82.6 bits (174), Expect(3) = 1e-033
Identities = 33/50 (66%), Positives = 39/50 (78%)
Frame = -2 / +2
Query: 706 GLTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
GLT ++Y+HDV+AGT+PTA+RIAQ A FGAVVAIDDPLTTGP
Sbjct: 104 GLTKFKLYWHDVLAGTSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTGP 253
Score = 79.4 bits (167), Expect(3) = 4e-027
Identities = 33/50 (66%), Positives = 41/50 (82%)
Frame = +3 / -1
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVVNP 707
GPVVSGSSMATTAPK V+++L DA CA+R AVG VPA+TS +Y+ +V P
Sbjct: 252 GPVVSGSSMATTAPKKVEELLVDAACAIRIAVGLVPASTSCQYSLNLVRP 103
Score = 60.2 bits (125), Expect(3) = 1e-033
Identities = 25/35 (71%), Positives = 27/35 (77%)
Frame = -2 / +2
Query: 553 RAAGTEVGRAQGTYTFADQQTFGLLMVMNFVFTAG 449
R + E+GRAQGTYTFADQ TFGLLM MNFV G
Sbjct: 269 RQSKDEIGRAQGTYTFADQATFGLLMNMNFVLPPG 373
Score = 51.9 bits (107), Expect(3) = 4e-027
Identities = 22/30 (73%), Positives = 22/30 (73%)
Frame = +3 / -1
Query: 450 PAVNTKFMTMRRPNVCWSANV*VPWARPTS 539
P TKFM MRRP V WSANV*VP ARP S
Sbjct: 372 PGGKTKFMFMRRPKVAWSANV*VPCARPIS 283
Score = 38.2 bits (77), Expect(3) = 1e-033
Identities = 18/23 (78%), Positives = 19/23 (82%)
Frame = -2 / +2
Query: 427 SILGRNEVLSDVREMSIVGGSGK 359
S L NEVLS VREMSIVGG+GK
Sbjct: 392 SHLRPNEVLSAVREMSIVGGTGK 460
Score = 27.6 bits (54), Expect(3) = 4e-027
Identities = 11/25 (44%), Positives = 13/25 (52%)
Frame = +3 / -1
Query: 357 NLPLPPTMLIXXXXXXXXXXPRMES 431
NLP+PPTMLI R E+
Sbjct: 462 NLPVPPTMLISLTADSTSFGRRCEA 388
>gb|CA028103.1|CA028103 HZ61A03r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ61A03
5-PRIME, mRNA sequence
Length = 627
Score = 100 bits (212), Expect(3) = 6e-029
Identities = 42/90 (46%), Positives = 59/90 (65%)
Frame = -2 / +2
Query: 535 VGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGKF 356
+GRAQG Y A + + LLM MNFVF G +NGS+++I+G N+ VREM +VGG+G F
Sbjct: 347 LGRAQGIYVSAGKDSLSLLMAMNFVFVDGAYNGSSIAIIGPNQADRAVREMPVVGGTGIF 526
Query: 355 RMARGYVQAHTIDSGATSGTTVVQYTVNVK 266
R ARGY Q T A +G V+Y ++++
Sbjct: 527 RFARGYCQLRTHWFDAKTGDATVEYKIHLR 616
Score = 42.3 bits (86), Expect(3) = 6e-029
Identities = 17/39 (43%), Positives = 20/39 (51%)
Frame = -2 / +2
Query: 673 VVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
V G N V++AQ FG V+ IDDPLT GP
Sbjct: 212 VSGGPNARVVQVAQAPSSNASATGFGTVLVIDDPLTEGP 328
Score = 22.6 bits (43), Expect(3) = 6e-029
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = -2 / +2
Query: 700 TTSEVYFHDVVAG 662
T VY+HDVV+G
Sbjct: 182 TRLRVYWHDVVSG 220
>gb|BF065894.2|BF065894 HV_CEb0014E14f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0014E14f, mRNA sequence
Length = 948
Score = 99.1 bits (210), Expect(3) = 1e-028
Identities = 42/88 (47%), Positives = 57/88 (64%)
Frame = -2 / +3
Query: 535 VGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGKF 356
+GRAQG Y A + + LLM MNFVF G +NGS+++I+G N+ VREM +VGG+G F
Sbjct: 342 LGRAQGIYVSAGKDSLSLLMAMNFVFVDGAYNGSSIAIIGPNQADRAVREMPVVGGTGVF 521
Query: 355 RMARGYVQAHTIDSGATSGTTVVQYTVN 272
R ARGY Q T A +G V+Y ++
Sbjct: 522 RFARGYCQLRTHWFDAKTGDATVEYKIH 605
Score = 42.3 bits (86), Expect(3) = 1e-028
Identities = 17/39 (43%), Positives = 20/39 (51%)
Frame = -2 / +3
Query: 673 VVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
V G N V++AQ FG V+ IDDPLT GP
Sbjct: 207 VSGGPNARVVQVAQAPSSNASATGFGTVLVIDDPLTEGP 323
Score = 22.6 bits (43), Expect(3) = 1e-028
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = -2 / +3
Query: 700 TTSEVYFHDVVAG 662
T VY+HDVV+G
Sbjct: 177 TRLRVYWHDVVSG 215
>gb|BF064827.1|BF064827 HV_CEb0019M01f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0019M01f, mRNA sequence
Length = 604
Score = 90.4 bits (191), Expect(3) = 4e-026
Identities = 40/83 (48%), Positives = 52/83 (62%)
Frame = -2 / +1
Query: 535 VGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGKF 356
+GRAQG Y A + + LLM MNFVF G +NGS+++I+G N+ VREM +VGG+G
Sbjct: 355 LGRAQGIYVSAGKDSLSLLMAMNFVFVDGAYNGSSIAIIGPNQADRAVREMPVVGGTGVX 534
Query: 355 RMARGYVQAHTIDSGATSGTTVV 287
R ARGY Q T A +G V
Sbjct: 535 RFARGYCQLRTHWFDAKTGDATV 603
Score = 42.3 bits (86), Expect(3) = 4e-026
Identities = 17/39 (43%), Positives = 20/39 (51%)
Frame = -2 / +1
Query: 673 VVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
V G N V++AQ FG V+ IDDPLT GP
Sbjct: 220 VSGGPNARVVQVAQAPSSNASATGFGTVLVIDDPLTEGP 336
Score = 22.6 bits (43), Expect(3) = 4e-026
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = -2 / +1
Query: 700 TTSEVYFHDVVAG 662
T VY+HDVV+G
Sbjct: 190 TRLRVYWHDVVSG 228
>gb|BG300692.2|BG300692 HVSMEb0018A09f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0018A09f, mRNA sequence
Length = 611
Score = 106 bits (227), Expect(2) = 8e-026
Identities = 48/93 (51%), Positives = 57/93 (61%)
Frame = -2 / +1
Query: 601 FGAVVAIDDPLTTGPTRAAGTEVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSI 422
F +V + D T VGRAQG Y ADQ G L MN V T+G +NGSTL++
Sbjct: 172 FFGMVNVMDDPLTEGPEPGSAAVGRAQGLYMGADQAQLGFLQTMNLVLTSGPYNGSTLAV 351
Query: 421 LGRNEVLSDVREMSIVGGSGKFRMARGYVQAHT 323
LGRN L+DVREM +VGG+G FR A GY QA T
Sbjct: 352 LGRNCPLTDVREMPVVGGTGAFRFASGYAQART 450
Score = 29.9 bits (59), Expect(2) = 8e-026
Identities = 9/16 (56%), Positives = 14/16 (87%)
Frame = -2 / +1
Query: 685 YFHDVVAGTNPTAVRI 638
+FHD V+G +P+AVR+
Sbjct: 94 FFHDTVSGASPSAVRV 141
>gb|CA026158.1|CA026158 HZ55B18r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ55B18
5-PRIME, mRNA sequence
Length = 333
Score = 80.3 bits (169), Expect(2) = 1e-025
Identities = 32/50 (64%), Positives = 38/50 (76%)
Frame = -2 / +2
Query: 706 GLTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
GLT ++Y+HDV+AGT+PTA+RIAQ A FGAVVA DDPLTTGP
Sbjct: 77 GLTKFKLYWHDVLAGTSPTAIRIAQAASTNSSSTFFGAVVAXDDPLTTGP 226
Score = 79.4 bits (167), Expect(2) = 1e-023
Identities = 33/50 (66%), Positives = 41/50 (82%)
Frame = +3 / -1
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVVNP 707
GPVVSGSSMATTAPK V+++L DA CA+R AVG VPA+TS +Y+ +V P
Sbjct: 225 GPVVSGSSMATTAPKKVEELLVDAACAIRIAVGLVPASTSCQYSLNLVRP 76
Score = 55.6 bits (115), Expect(2) = 1e-025
Identities = 22/25 (88%), Positives = 23/25 (92%)
Frame = -2 / +2
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNF 464
E+GRAQGTYTFADQ TFGLLM MNF
Sbjct: 257 EIGRAQGTYTFADQATFGLLMNMNF 331
Score = 50.1 bits (103), Expect(2) = 1e-023
Identities = 21/26 (80%), Positives = 21/26 (80%)
Frame = +3 / -1
Query: 462 TKFMTMRRPNVCWSANV*VPWARPTS 539
TKFM MRRP V WSANV*VP ARP S
Sbjct: 333 TKFMFMRRPKVAWSANV*VPCARPIS 256
>gb|AV922971.1|AV922971 AV922971 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd3d02 5', mRNA sequence
Length = 653
Score = 100 bits (213), Expect(3) = 5e-025
Identities = 41/94 (43%), Positives = 59/94 (62%)
Frame = -2 / +2
Query: 577 DPLTTGPTRAAGTEVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLS 398
D T + VGRAQG Y + Q L+M +NF+ T G++NGSTL+++GR+++++
Sbjct: 203 DDALTETSSPTSAPVGRAQGFYMMSSQSGMVLMMCVNFLLTTGDYNGSTLAVIGRDDIMT 382
Query: 397 DVREMSIVGGSGKFRMARGYVQAHTIDSGATSGT 296
RE+S+VGG+GKFRMA GYV T S T
Sbjct: 383 TTRELSVVGGTGKFRMATGYVLWKTNSSKGADAT 484
Score = 32.2 bits (64), Expect(3) = 5e-025
Identities = 11/16 (68%), Positives = 15/16 (93%)
Frame = -2 / +2
Query: 685 YFHDVVAGTNPTAVRI 638
+ HDVV+G+NPTAV+I
Sbjct: 89 FLHDVVSGSNPTAVQI 136
Score = 21.7 bits (41), Expect(3) = 5e-025
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = -1 / +1
Query: 608 HQLRRGGGHRRPAD 567
H LRR HRR AD
Sbjct: 175 HGLRRHDRHRRRAD 216
>gb|CA029181.1|CA029181 HZ64H06r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ64H06
5-PRIME, mRNA sequence
Length = 369
Score = 78.0 bits (164), Expect(2) = 5e-025
Identities = 32/49 (65%), Positives = 36/49 (73%)
Frame = -2 / +2
Query: 703 LTTSEVYFHDVVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
LT +VYFHDV+AG +PTA+RIAQ A FGAVVAIDDPLTT P
Sbjct: 116 LTKFKVYFHDVLAGKSPTAIRIAQAASTNSSSTFFGAVVAIDDPLTTSP 262
Score = 78.0 bits (164), Expect(2) = 3e-023
Identities = 33/48 (68%), Positives = 38/48 (79%)
Frame = +3 / -1
Query: 558 GPVVSGSSMATTAPKLVDDVLEDAXCAMRTAVGFVPATTSWKYTSEVV 701
G VVSGSSMATTAPK V+++L DA CA+R AVG PA TSWKYT +V
Sbjct: 261 GLVVSGSSMATTAPKKVEELLVDAACAIRIAVGLFPARTSWKYTLNLV 118
Score = 56.1 bits (116), Expect(2) = 5e-025
Identities = 23/25 (92%), Positives = 23/25 (92%)
Frame = -2 / +2
Query: 538 EVGRAQGTYTFADQQTFGLLMVMNF 464
EVGRAQGTYTFADQ TFGLLM MNF
Sbjct: 293 EVGRAQGTYTFADQATFGLLMNMNF 367
Score = 50.1 bits (103), Expect(2) = 3e-023
Identities = 21/26 (80%), Positives = 21/26 (80%)
Frame = +3 / -1
Query: 462 TKFMTMRRPNVCWSANV*VPWARPTS 539
TKFM MR P V WSANV*VP ARPTS
Sbjct: 369 TKFMFMRSPKVAWSANV*VPCARPTS 292
>gb|BI958606.1|BI958606 HVSMEn0015N17f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0015N17f, mRNA sequence
Length = 646
Score = 97.7 bits (207), Expect(3) = 3e-024
Identities = 40/94 (42%), Positives = 58/94 (61%)
Frame = -2 / +1
Query: 577 DPLTTGPTRAAGTEVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLS 398
D T + VGRAQG Y + Q L+M +NF+ T G++NGSTL+++GR+++++
Sbjct: 196 DDALTETSSPTSAPVGRAQGFYMMSSQSGMVLMMCVNFLLTTGDYNGSTLAVIGRDDIMT 375
Query: 397 DVREMSIVGGSGKFRMARGYVQAHTIDSGATSGT 296
RE+S+VGG+GKF MA GYV T S T
Sbjct: 376 TTRELSVVGGTGKFXMATGYVLWKTNSSKGADAT 477
Score = 32.2 bits (64), Expect(3) = 3e-024
Identities = 11/16 (68%), Positives = 15/16 (93%)
Frame = -2 / +1
Query: 685 YFHDVVAGTNPTAVRI 638
+ HDVV+G+NPTAV+I
Sbjct: 82 FLHDVVSGSNPTAVQI 129
Score = 21.7 bits (41), Expect(3) = 3e-024
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = -1 / +3
Query: 608 HQLRRGGGHRRPAD 567
H LRR HRR AD
Sbjct: 168 HGLRRHDRHRRRAD 209
>gb|BE215009.2|BE215009 HV_CEb0005I10f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0005I10f, mRNA sequence
Length = 687
Score = 88.6 bits (187), Expect(2) = 8e-024
Identities = 36/66 (54%), Positives = 47/66 (71%)
Frame = -2 / +3
Query: 535 VGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGKF 356
+GRAQG Y A + + LLM MNFVF G +NGS+++I+G N+ VREM +VGG+G F
Sbjct: 333 LGRAQGIYVSAGKDSLSLLMAMNFVFVDGAYNGSSIAIIGPNQADRAVREMPVVGGTGVF 512
Query: 355 RMARGY 338
R ARGY
Sbjct: 513 RFARGY 530
Score = 41.4 bits (84), Expect(2) = 8e-024
Identities = 17/39 (43%), Positives = 20/39 (51%)
Frame = -2 / +3
Query: 673 VVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
V G N V++AQ FG V+ IDDPLT GP
Sbjct: 198 VSGGPNARVVQVAQAPFSNASDTGFGTVLVIDDPLTEGP 314
>gb|BI959140.1|BI959140 HVSMEn0018F14f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0018F14f, mRNA sequence
Length = 645
Score = 88.6 bits (187), Expect(3) = 1e-021
Identities = 34/85 (40%), Positives = 54/85 (63%)
Frame = -2 / +2
Query: 577 DPLTTGPTRAAGTEVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLS 398
D T + VGRAQG Y + Q L+M +NF+ T G++NGSTL+++GR+++++
Sbjct: 203 DDALTETSSPTSAPVGRAQGFYMMSSQSGMVLMMCVNFLLTTGDYNGSTLAVIGRDDIMT 382
Query: 397 DVREMSIVGGSGKFRMARGYVQAHT 323
RE+S+VGG+GK+ MA + T
Sbjct: 383 TTRELSVVGGTGKYFMATANINCKT 457
Score = 32.2 bits (64), Expect(3) = 1e-021
Identities = 11/16 (68%), Positives = 15/16 (93%)
Frame = -2 / +2
Query: 685 YFHDVVAGTNPTAVRI 638
+ HDVV+G+NPTAV+I
Sbjct: 89 FLHDVVSGSNPTAVQI 136
Score = 21.7 bits (41), Expect(3) = 1e-021
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = -1 / +1
Query: 608 HQLRRGGGHRRPAD 567
H LRR HRR AD
Sbjct: 175 HGLRRHDRHRRRAD 216
>gb|BQ768104.1|BQ768104 EBro08_SQ010_O16_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ010_O16 5', mRNA sequence
Length = 444
Score = 101 bits (216), Expect = 3e-021
Identities = 43/90 (47%), Positives = 59/90 (65%)
Frame = -2 / +3
Query: 535 VGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGKF 356
+GRAQG Y A + + LLM MNFVF G +NGS+++I+G N V VREM++ GG+G F
Sbjct: 21 LGRAQGVYVSAGKDSLSLLMAMNFVFVDGAYNGSSIAIVGANPVNRAVREMAVAGGTGVF 200
Query: 355 RMARGYVQAHTIDSGATSGTTVVQYTVNVK 266
R ARGY Q T A +G V+Y V+++
Sbjct: 201 RFARGYCQLRTRWFDANTGDATVEYRVHLR 290
>gb|BF620088.2|BF620088 HVSMEc0011N11f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0011N11f, mRNA sequence
Length = 429
Score = 100 bits (213), Expect(2) = 4e-021
Identities = 41/94 (43%), Positives = 59/94 (62%)
Frame = -2 / +1
Query: 577 DPLTTGPTRAAGTEVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLS 398
D T + VGRAQG Y + Q L+M +NF+ T G++NGSTL+++GR+++++
Sbjct: 46 DDALTETSSPTSAPVGRAQGFYMMSSQSGMVLMMCVNFLLTTGDYNGSTLAVIGRDDIMT 225
Query: 397 DVREMSIVGGSGKFRMARGYVQAHTIDSGATSGT 296
RE+S+VGG+GKFRMA GYV T S T
Sbjct: 226 TTRELSVVGGTGKFRMATGYVLWKTNSSKGADAT 327
Score = 21.7 bits (41), Expect(2) = 4e-021
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = -1 / +3
Query: 608 HQLRRGGGHRRPAD 567
H LRR HRR AD
Sbjct: 18 HGLRRHDRHRRRAD 59
>gb|BI958965.1|BI958965 HVSMEn0017G24f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0017G24f, mRNA sequence
Length = 658
Score = 86.8 bits (183), Expect(3) = 5e-021
Identities = 33/79 (41%), Positives = 52/79 (65%)
Frame = -2 / +2
Query: 577 DPLTTGPTRAAGTEVGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLS 398
D T + VGRAQG Y + Q L+M +NF+ T G++NGSTL+++GR+++++
Sbjct: 191 DDALTETSSPTSAPVGRAQGFYMMSSQSGMVLMMCVNFLLTTGDYNGSTLAVIGRDDIMT 370
Query: 397 DVREMSIVGGSGKFRMARG 341
RE+S+VGG+GK A+G
Sbjct: 371 TTRELSVVGGTGKXXXAKG 427
Score = 32.2 bits (64), Expect(3) = 5e-021
Identities = 11/16 (68%), Positives = 15/16 (93%)
Frame = -2 / +2
Query: 685 YFHDVVAGTNPTAVRI 638
+ HDVV+G+NPTAV+I
Sbjct: 77 FLHDVVSGSNPTAVQI 124
Score = 21.7 bits (41), Expect(3) = 5e-021
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = -1 / +1
Query: 608 HQLRRGGGHRRPAD 567
H LRR HRR AD
Sbjct: 163 HGLRRHDRHRRRAD 204
>gb|BF623292.1|BF623292 HVSMEa0015C23f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0015C23f, mRNA sequence
Length = 830
Score = 73.0 bits (153), Expect(3) = 5e-021
Identities = 31/65 (47%), Positives = 43/65 (66%)
Frame = -2 / +3
Query: 535 VGRAQGTYTFADQQTFGLLMVMNFVFTAGEHNGSTLSILGRNEVLSDVREMSIVGGSGKF 356
+GRAQG Y A + + LLM MNF+F G +NGS++ I+G N+ VREM I G+G
Sbjct: 339 LGRAQGIYVSAGKDSLSLLMDMNFIFDNGAYNGSSIDIIGPNQADRTVREMPIKDGTGIL 518
Query: 355 RMARG 341
++ARG
Sbjct: 519 QIARG 533
Score = 42.3 bits (86), Expect(3) = 5e-021
Identities = 17/39 (43%), Positives = 20/39 (51%)
Frame = -2 / +3
Query: 673 VVAGTNPTAVRIAQXAXXXXXXXNFGAVVAIDDPLTTGP 557
V G N V++AQ FG V+ IDDPLT GP
Sbjct: 204 VSGGPNARVVQVAQAPSSNASATGFGTVLVIDDPLTEGP 320
Score = 22.6 bits (43), Expect(3) = 5e-021
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = -2 / +3
Query: 700 TTSEVYFHDVVAG 662
T VY+HDVV+G
Sbjct: 174 TRLRVYWHDVVSG 212
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 227,352,432
Number of Sequences: 312970
Number of extensions: 3080064
Number of successful extensions: 57355
Number of sequences better than 1.0e-020: 61
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7804674456
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)