TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3438088.2.1
(615 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ760319.1|BQ760319 EBpi07_SQ003_L24_R pistil, 12 DPA, n... 137 7e-049
gb|CB875548.1|CB875548 HX08K06w HX Hordeum vulgare subsp. v... 137 3e-048
dbj|AB040769.1| Hordeum vulgare mRNA for endo-1,4-beta-gluc... 166 9e-042
gb|AW982232.3|AW982232 HVSMEg0002G13f Hordeum vulgare pre-a... 166 9e-042
gb|BG418595.1|BG418595 HVSMEk0023G06f Hordeum vulgare testa... 166 9e-042
gb|AV927273.1|AV927273 AV927273 K. Sato unpublished cDNA li... 166 9e-042
gb|BE601584.3|BE601584 HVSMEh0098M02f Hordeum vulgare 5-45 ... 166 7e-041
gb|BM097444.2|BM097444 EBro05_SQ003_F03_R root, 3 week, red... 166 7e-041
gb|AW983004.3|AW983004 HVSMEg0004M14f Hordeum vulgare pre-a... 165 3e-040
gb|AV916435.1|AV916435 AV916435 K. Sato unpublished cDNA li... 164 4e-040
gb|CB859220.1|CB859220 HI09M02w HI Hordeum vulgare subsp. v... 133 6e-037
gb|BU994975.1|BU994975 HM08M21r HM Hordeum vulgare subsp. v... 136 9e-035
gb|AV923747.1|AV923747 AV923747 K. Sato unpublished cDNA li... 144 6e-034
gb|AV920035.1|AV920035 AV920035 K. Sato unpublished cDNA li... 140 8e-034
gb|AV917955.1|AV917955 AV917955 K. Sato unpublished cDNA li... 138 4e-033
gb|BJ456192.1|BJ456192 BJ456192 K. Sato unpublished cDNA li... 138 4e-033
gb|BU985662.1|BU985662 HF08E02r HF Hordeum vulgare subsp. v... 141 4e-033
gb|BQ768653.1|BQ768653 EBro08_SQ012_F07_R root, 3 week, dro... 136 9e-032
gb|BQ764603.1|BQ764603 EBca01_SQ004_I10_R carpel, pre-anthe... 115 2e-026
gb|AV912096.1|AV912096 AV912096 K. Sato unpublished cDNA li... 91 1e-025
>gb|BQ760319.1|BQ760319 EBpi07_SQ003_L24_R pistil, 12 DPA, no treatment, cv Optic, EBpi07
Hordeum vulgare subsp. vulgare cDNA clone
EBpi07_SQ003_L24 5', mRNA sequence
Length = 620
Score = 137 bits (293), Expect(3) = 7e-049
Identities = 57/71 (80%), Positives = 59/71 (83%)
Frame = +3 / -1
Query: 318 PWPSPDKATNAATRPALPASVASV*L*FLRASLNPSWRSGPATMAPAMVLGFGFLASLHF 497
PWPSPDKATNAATRPALPA V SV*L F R SLNPSWRSGPAT+AP M+ G GFL SLH
Sbjct: 398 PWPSPDKATNAATRPALPARVGSV*LRFFRMSLNPSWRSGPATIAPTMM*GLGFLVSLHV 219
Query: 498 HPPLQP**TPF 530
HPPLQP * PF
Sbjct: 218 HPPLQPG*IPF 186
Score = 110 bits (235), Expect(3) = 5e-046
Identities = 40/58 (68%), Positives = 44/58 (75%)
Frame = -3 / +1
Query: 556 HHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGPDRHDGFKDARRNYNYTE 383
H + K G+H GCKGGW WRD KKPNP+ I GAMVAGPDRHDGFKD R+N NYTE
Sbjct: 160 HRGASIPKKKGIHPGCKGGWTWRDTKKPNPHIIVGAMVAGPDRHDGFKDIRKNRNYTE 333
Score = 87.7 bits (185), Expect(2) = 3e-022
Identities = 42/72 (58%), Positives = 51/72 (70%)
Frame = +2 / -3
Query: 317 AVAVTRQSYQCGD*TSVARQRCFSVVVVPAGIFEPVMAVGASNHGTCYGIRVWLLGVPPL 496
A+A TR SYQ +*TS+AR F VV V + +FEPVMA+ AS+H + +RV LLGVPP
Sbjct: 399 AMAFTR*SYQRSN*TSIAR*GWFCVVAVLSDVFEPVMAIRASHHSSNNDVRVGLLGVPPR 220
Query: 497 PPSFAAIVNTVL 532
PP FAA VNT L
Sbjct: 219 PPPFAARVNTFL 184
Score = 76.7 bits (161), Expect(3) = 1e-027
Identities = 42/72 (58%), Positives = 43/72 (59%)
Frame = -1 / +2
Query: 531 RTVFTMAAKEGGSGGTPRSQTLIP*QVPWLLAPTAMTGSKMPAGTTTTLKQRWRATLV*S 352
R VFT+AAK GG GGTPRS TL W LA AMTGSK T TT Q RA LV*
Sbjct: 185 RKVFTLAAKGGGRGGTPRSPTLTSLLELWWLALIAMTGSKTSERTATTQNQP*RAMLV*L 364
Query: 351 PHW*LCRVTATA 316
W*L RV A A
Sbjct: 365 LRW*LYRVKAMA 400
Score = 72.1 bits (151), Expect(2) = 7e-021
Identities = 28/45 (62%), Positives = 32/45 (71%)
Frame = +1 / -2
Query: 313 PRRGRHPTKLPMRRLDQRCPPALLQCSCSSCGHL*TRHGGRGQQP 447
PR G HP KLP ++LDQ CP L+ CSC S G L*TRHG +GQ P
Sbjct: 403 PRHGLHPIKLPTQQLDQHCPLGLVLCSCGSFGCL*TRHGDQGQPP 269
Score = 63.8 bits (133), Expect(3) = 5e-046
Identities = 24/28 (85%), Positives = 25/28 (89%)
Frame = -3 / +1
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPK 530
NPLKMSYVVG GK YP+ VHHRGASIPK
Sbjct: 100 NPLKMSYVVGHGKHYPKRVHHRGASIPK 183
Score = 48.7 bits (100), Expect(3) = 7e-049
Identities = 23/29 (79%), Positives = 24/29 (82%)
Frame = +3 / -1
Query: 528 FLGIEAPRW*TCLG*RFPNPTT*LIFKGF 614
F GI+APR *T LG* FP PTT*LIFKGF
Sbjct: 185 FFGIDAPR**TRLG*CFPCPTT*LIFKGF 99
Score = 48.3 bits (99), Expect(3) = 5e-046
Identities = 18/28 (64%), Positives = 22/28 (78%)
Frame = -2 / +3
Query: 311 GQEHHVLCSTAHVPFASAAAGSMETVTA 228
GQEHHVLC A+VPFA AA+ +ME + A
Sbjct: 402 GQEHHVLCCAANVPFAPAASSTMEAIMA 485
Score = 47.8 bits (98), Expect(2) = 7e-021
Identities = 21/42 (50%), Positives = 26/42 (61%)
Frame = +2 / -3
Query: 488 PPLPPSFAAIVNTVLRDRGAPVVNMPGVTLSEPNHVAHLQRI 613
P PP A + +LRDR V+N GV LS P+H+ HLQRI
Sbjct: 225 PRPPPFAARVNTFLLRDRRTTVMNTLGVMLSVPDHITHLQRI 100
Score = 46.4 bits (95), Expect(3) = 7e-049
Identities = 19/29 (65%), Positives = 20/29 (68%)
Frame = +1 / -2
Query: 226 QAVTVSMEPAAAEAKGTWAVLQRTWCSCP 312
QA+ SM AA AKGT A QRTWCSCP
Sbjct: 487 QAIMASMVLEAAGAKGTLAAQQRTWCSCP 401
Score = 46.0 bits (94), Expect(3) = 1e-027
Identities = 20/29 (68%), Positives = 22/29 (75%)
Frame = -2 / +3
Query: 614 ESFEDELRGWVRKALPQACSPPGRLDP*E 528
+SFEDEL G RKALPQACS P +DP E
Sbjct: 99 KSFEDELCGRARKALPQACSSPWCVDPEE 185
Score = 39.6 bits (80), Expect(3) = 1e-027
Identities = 15/29 (51%), Positives = 17/29 (58%)
Frame = -1 / +2
Query: 312 WTRTPCSLQYRPCSXXXXXXXLHGNRDGL 226
WTRTPCSL R CS HG+ +GL
Sbjct: 401 WTRTPCSLLCRQCSLRPRRLQHHGSHNGL 487
Score = 36.8 bits (74), Expect(2) = 3e-022
Identities = 13/17 (76%), Positives = 15/17 (88%)
Frame = +1 / -2
Query: 562 AWGNAFRTQPRSSSSKD 612
AWGNAFR +P +SSSKD
Sbjct: 151 AWGNAFRARPHNSSSKD 101
>gb|CB875548.1|CB875548 HX08K06w HX Hordeum vulgare subsp. vulgare cDNA clone HX08K06
3-PRIME, mRNA sequence
Length = 620
Score = 137 bits (293), Expect(3) = 3e-048
Identities = 57/71 (80%), Positives = 59/71 (83%)
Frame = +3 / +2
Query: 318 PWPSPDKATNAATRPALPASVASV*L*FLRASLNPSWRSGPATMAPAMVLGFGFLASLHF 497
PWPSPDKATNAATRPALPA V SV*L F R SLNPSWRSGPAT+AP M+ G GFL SLH
Sbjct: 281 PWPSPDKATNAATRPALPARVGSV*LRFFRMSLNPSWRSGPATIAPTMM*GLGFLVSLHV 460
Query: 498 HPPLQP**TPF 530
HPPLQP * PF
Sbjct: 461 HPPLQPG*IPF 493
Score = 110 bits (235), Expect(3) = 5e-046
Identities = 40/58 (68%), Positives = 44/58 (75%)
Frame = -3 / -3
Query: 556 HHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGPDRHDGFKDARRNYNYTE 383
H + K G+H GCKGGW WRD KKPNP+ I GAMVAGPDRHDGFKD R+N NYTE
Sbjct: 519 HRGASIPKKKGIHPGCKGGWTWRDTKKPNPHIIVGAMVAGPDRHDGFKDIRKNRNYTE 346
Score = 96.4 bits (204), Expect(2) = 1e-027
Identities = 44/82 (53%), Positives = 55/82 (67%)
Frame = -2 / -2
Query: 530 ERCSLWLQRRVEVEGRQEAKP*YHSRCHGCWXXXXXRVQRCPQELQLH*SNAGGQRWSSR 351
ER S WLQR V+VEG QEA+P +H +G W RVQR P+E QLH +N GQ WSS
Sbjct: 493 ERYSPWLQRGVDVEGHQEAQPSHHCWSYGGWP*SP*RVQRHPKEPQLHRTNPSGQCWSSC 314
Query: 350 RIGSFVG*RPRRGGQEHHVLCS 285
+GSF+G*RP RG + H + C+
Sbjct: 313 CVGSFIG*RPWRGQEHHVLCCA 248
Score = 87.7 bits (185), Expect(2) = 3e-022
Identities = 42/72 (58%), Positives = 51/72 (70%)
Frame = +2 / +1
Query: 317 AVAVTRQSYQCGD*TSVARQRCFSVVVVPAGIFEPVMAVGASNHGTCYGIRVWLLGVPPL 496
A+A TR SYQ +*TS+AR F VV V + +FEPVMA+ AS+H + +RV LLGVPP
Sbjct: 280 AMAFTR*SYQRSN*TSIAR*GWFCVVAVLSDVFEPVMAIRASHHSSNNDVRVGLLGVPPR 459
Query: 497 PPSFAAIVNTVL 532
PP FAA VNT L
Sbjct: 460 PPPFAARVNTFL 495
Score = 72.1 bits (151), Expect(2) = 7e-021
Identities = 28/45 (62%), Positives = 32/45 (71%)
Frame = +1 / +3
Query: 313 PRRGRHPTKLPMRRLDQRCPPALLQCSCSSCGHL*TRHGGRGQQP 447
PR G HP KLP ++LDQ CP L+ CSC S G L*TRHG +GQ P
Sbjct: 276 PRHGLHPIKLPTQQLDQHCPLGLVLCSCGSFGCL*TRHGDQGQPP 410
Score = 63.8 bits (133), Expect(3) = 5e-046
Identities = 24/28 (85%), Positives = 25/28 (89%)
Frame = -3 / -3
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPK 530
NPLKMSYVVG GK YP+ VHHRGASIPK
Sbjct: 579 NPLKMSYVVGHGKHYPKRVHHRGASIPK 496
Score = 48.7 bits (100), Expect(3) = 3e-048
Identities = 23/29 (79%), Positives = 24/29 (82%)
Frame = +3 / +2
Query: 528 FLGIEAPRW*TCLG*RFPNPTT*LIFKGF 614
F GI+APR *T LG* FP PTT*LIFKGF
Sbjct: 494 FFGIDAPR**TRLG*CFPCPTT*LIFKGF 580
Score = 48.3 bits (99), Expect(3) = 5e-046
Identities = 18/28 (64%), Positives = 22/28 (78%)
Frame = -2 / -2
Query: 311 GQEHHVLCSTAHVPFASAAAGSMETVTA 228
GQEHHVLC A+VPFA AA+ +ME + A
Sbjct: 277 GQEHHVLCCAANVPFAPAASSTMEAIMA 194
Score = 47.8 bits (98), Expect(2) = 7e-021
Identities = 21/42 (50%), Positives = 26/42 (61%)
Frame = +2 / +1
Query: 488 PPLPPSFAAIVNTVLRDRGAPVVNMPGVTLSEPNHVAHLQRI 613
P PP A + +LRDR V+N GV LS P+H+ HLQRI
Sbjct: 454 PRPPPFAARVNTFLLRDRRTTVMNTLGVMLSVPDHITHLQRI 579
Score = 46.0 bits (94), Expect(2) = 1e-027
Identities = 20/29 (68%), Positives = 22/29 (75%)
Frame = -2 / -2
Query: 614 ESFEDELRGWVRKALPQACSPPGRLDP*E 528
+SFEDEL G RKALPQACS P +DP E
Sbjct: 580 KSFEDELCGRARKALPQACSSPWCVDPEE 494
Score = 44.1 bits (90), Expect(3) = 3e-048
Identities = 18/28 (64%), Positives = 19/28 (67%)
Frame = +1 / +3
Query: 229 AVTVSMEPAAAEAKGTWAVLQRTWCSCP 312
A+ SM AA AKGT A QRTWCSCP
Sbjct: 195 AIMASMVLEAAGAKGTLAAQQRTWCSCP 278
Score = 36.8 bits (74), Expect(2) = 3e-022
Identities = 13/17 (76%), Positives = 15/17 (88%)
Frame = +1 / +3
Query: 562 AWGNAFRTQPRSSSSKD 612
AWGNAFR +P +SSSKD
Sbjct: 528 AWGNAFRARPHNSSSKD 578
>dbj|AB040769.1| Hordeum vulgare mRNA for endo-1,4-beta-glucanase Cel1, complete cds
Length = 2442
Score = 166 bits (358), Expect(2) = 9e-042
Identities = 61/77 (79%), Positives = 68/77 (88%)
Frame = -3 / +1
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP KMSYVVGFGK+YP+ VHHRGASIP NGV YGCKGG+KWR++KK NPN + GAMVAGP
Sbjct: 1666 NPQKMSYVVGFGKKYPKRVHHRGASIPHNGVKYGCKGGFKWRESKKANPNILVGAMVAGP 1845
Query: 433 DRHDGFKDARRNYNYTE 383
D+HDGFKD R NYNYTE
Sbjct: 1846 DKHDGFKDIRTNYNYTE 1896
Score = 124 bits (265), Expect = 5e-028
Identities = 61/93 (65%), Positives = 65/93 (69%)
Frame = +3 / -2
Query: 336 KATNAATRPALPASVASV*L*FLRASLNPSWRSGPATMAPAMVLGFGFLASLHFHPPLQP 515
K NAATRPA A V SV*L*F+R SL PS SGPAT+AP +LGF FL SLH PPL P
Sbjct: 1943 KEINAATRPAFAARVGSV*L*FVRISLKPSCLSGPATIAPTRILGFAFLDSLHLKPPLHP 1764
Query: 516 **TPFLGIEAPRW*TCLG*RFPNPTT*LIFKGF 614
TP GI+AP *T LG FP PTT*LIF GF
Sbjct: 1763 YLTPL*GIDAPL**TRLGYFFPKPTT*LIFCGF 1665
Score = 23.1 bits (44), Expect(2) = 9e-042
Identities = 8/10 (80%), Positives = 10/10 (100%)
Frame = -3 / +1
Query: 313 VDKNTMFSAV 284
+DKNT+FSAV
Sbjct: 1972 IDKNTIFSAV 2001
>gb|AW982232.3|AW982232 HVSMEg0002G13f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0002G13f, mRNA sequence
Length = 850
Score = 166 bits (358), Expect(2) = 9e-042
Identities = 61/77 (79%), Positives = 68/77 (88%)
Frame = -3 / +3
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP KMSYVVGFGK+YP+ VHHRGASIP NGV YGCKGG+KWR++KK NPN + GAMVAGP
Sbjct: 162 NPQKMSYVVGFGKKYPKRVHHRGASIPHNGVKYGCKGGFKWRESKKANPNILVGAMVAGP 341
Query: 433 DRHDGFKDARRNYNYTE 383
D+HDGFKD R NYNYTE
Sbjct: 342 DKHDGFKDIRTNYNYTE 392
Score = 124 bits (265), Expect = 5e-028
Identities = 61/93 (65%), Positives = 65/93 (69%)
Frame = +3 / -1
Query: 336 KATNAATRPALPASVASV*L*FLRASLNPSWRSGPATMAPAMVLGFGFLASLHFHPPLQP 515
K NAATRPA A V SV*L*F+R SL PS SGPAT+AP +LGF FL SLH PPL P
Sbjct: 439 KEINAATRPAFAARVGSV*L*FVRISLKPSCLSGPATIAPTRILGFAFLDSLHLKPPLHP 260
Query: 516 **TPFLGIEAPRW*TCLG*RFPNPTT*LIFKGF 614
TP GI+AP *T LG FP PTT*LIF GF
Sbjct: 259 YLTPL*GIDAPL**TRLGYFFPKPTT*LIFCGF 161
Score = 23.1 bits (44), Expect(2) = 9e-042
Identities = 8/10 (80%), Positives = 10/10 (100%)
Frame = -3 / +3
Query: 313 VDKNTMFSAV 284
+DKNT+FSAV
Sbjct: 468 IDKNTIFSAV 497
>gb|BG418595.1|BG418595 HVSMEk0023G06f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0023G06f, mRNA sequence
Length = 815
Score = 166 bits (358), Expect(2) = 9e-042
Identities = 61/77 (79%), Positives = 68/77 (88%)
Frame = -3 / +3
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP KMSYVVGFGK+YP+ VHHRGASIP NGV YGCKGG+KWR++KK NPN + GAMVAGP
Sbjct: 186 NPQKMSYVVGFGKKYPKRVHHRGASIPHNGVKYGCKGGFKWRESKKANPNILVGAMVAGP 365
Query: 433 DRHDGFKDARRNYNYTE 383
D+HDGFKD R NYNYTE
Sbjct: 366 DKHDGFKDIRTNYNYTE 416
Score = 124 bits (265), Expect = 5e-028
Identities = 61/93 (65%), Positives = 65/93 (69%)
Frame = +3 / -2
Query: 336 KATNAATRPALPASVASV*L*FLRASLNPSWRSGPATMAPAMVLGFGFLASLHFHPPLQP 515
K NAATRPA A V SV*L*F+R SL PS SGPAT+AP +LGF FL SLH PPL P
Sbjct: 463 KEINAATRPAFAARVGSV*L*FVRISLKPSCLSGPATIAPTRILGFAFLDSLHLKPPLHP 284
Query: 516 **TPFLGIEAPRW*TCLG*RFPNPTT*LIFKGF 614
TP GI+AP *T LG FP PTT*LIF GF
Sbjct: 283 YLTPL*GIDAPL**TRLGYFFPKPTT*LIFCGF 185
Score = 23.1 bits (44), Expect(2) = 9e-042
Identities = 8/10 (80%), Positives = 10/10 (100%)
Frame = -3 / +3
Query: 313 VDKNTMFSAV 284
+DKNT+FSAV
Sbjct: 492 IDKNTIFSAV 521
>gb|AV927273.1|AV927273 AV927273 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd1e17 3', mRNA sequence
Length = 684
Score = 166 bits (358), Expect(2) = 9e-042
Identities = 61/77 (79%), Positives = 68/77 (88%)
Frame = -3 / -3
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP KMSYVVGFGK+YP+ VHHRGASIP NGV YGCKGG+KWR++KK NPN + GAMVAGP
Sbjct: 565 NPQKMSYVVGFGKKYPKRVHHRGASIPHNGVKYGCKGGFKWRESKKANPNILVGAMVAGP 386
Query: 433 DRHDGFKDARRNYNYTE 383
D+HDGFKD R NYNYTE
Sbjct: 385 DKHDGFKDIRTNYNYTE 335
Score = 124 bits (265), Expect = 5e-028
Identities = 61/93 (65%), Positives = 65/93 (69%)
Frame = +3 / +3
Query: 336 KATNAATRPALPASVASV*L*FLRASLNPSWRSGPATMAPAMVLGFGFLASLHFHPPLQP 515
K NAATRPA A V SV*L*F+R SL PS SGPAT+AP +LGF FL SLH PPL P
Sbjct: 288 KEINAATRPAFAARVGSV*L*FVRISLKPSCLSGPATIAPTRILGFAFLDSLHLKPPLHP 467
Query: 516 **TPFLGIEAPRW*TCLG*RFPNPTT*LIFKGF 614
TP GI+AP *T LG FP PTT*LIF GF
Sbjct: 468 YLTPL*GIDAPL**TRLGYFFPKPTT*LIFCGF 566
Score = 23.1 bits (44), Expect(2) = 9e-042
Identities = 8/10 (80%), Positives = 10/10 (100%)
Frame = -3 / -3
Query: 313 VDKNTMFSAV 284
+DKNT+FSAV
Sbjct: 259 IDKNTIFSAV 230
>gb|BE601584.3|BE601584 HVSMEh0098M02f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0098M02f, mRNA sequence
Length = 641
Score = 166 bits (358), Expect = 7e-041
Identities = 61/77 (79%), Positives = 68/77 (88%)
Frame = -3 / +1
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP KMSYVVGFGK+YP+ VHHRGASIP NGV YGCKGG+KWR++KK NPN + GAMVAGP
Sbjct: 217 NPQKMSYVVGFGKKYPKRVHHRGASIPHNGVKYGCKGGFKWRESKKANPNILVGAMVAGP 396
Query: 433 DRHDGFKDARRNYNYTE 383
D+HDGFKD R NYNYTE
Sbjct: 397 DKHDGFKDIRTNYNYTE 447
Score = 124 bits (265), Expect = 5e-028
Identities = 61/93 (65%), Positives = 65/93 (69%)
Frame = +3 / -1
Query: 336 KATNAATRPALPASVASV*L*FLRASLNPSWRSGPATMAPAMVLGFGFLASLHFHPPLQP 515
K NAATRPA A V SV*L*F+R SL PS SGPAT+AP +LGF FL SLH PPL P
Sbjct: 494 KEINAATRPAFAARVGSV*L*FVRISLKPSCLSGPATIAPTRILGFAFLDSLHLKPPLHP 315
Query: 516 **TPFLGIEAPRW*TCLG*RFPNPTT*LIFKGF 614
TP GI+AP *T LG FP PTT*LIF GF
Sbjct: 314 YLTPL*GIDAPL**TRLGYFFPKPTT*LIFCGF 216
>gb|BM097444.2|BM097444 EBro05_SQ003_F03_R root, 3 week, reduced light, cv Optic, EBro05
Hordeum vulgare subsp. vulgare cDNA clone
EBro05_SQ003_F03 5', mRNA sequence
Length = 499
Score = 166 bits (358), Expect = 7e-041
Identities = 61/77 (79%), Positives = 68/77 (88%)
Frame = -3 / +1
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP KMSYVVGFGK+YP+ VHHRGASIP NGV YGCKGG+KWR++KK NPN + GAMVAGP
Sbjct: 211 NPQKMSYVVGFGKKYPKRVHHRGASIPHNGVKYGCKGGFKWRESKKANPNILVGAMVAGP 390
Query: 433 DRHDGFKDARRNYNYTE 383
D+HDGFKD R NYNYTE
Sbjct: 391 DKHDGFKDIRTNYNYTE 441
Score = 124 bits (265), Expect = 5e-028
Identities = 61/93 (65%), Positives = 65/93 (69%)
Frame = +3 / -3
Query: 336 KATNAATRPALPASVASV*L*FLRASLNPSWRSGPATMAPAMVLGFGFLASLHFHPPLQP 515
K NAATRPA A V SV*L*F+R SL PS SGPAT+AP +LGF FL SLH PPL P
Sbjct: 488 KEINAATRPAFAARVGSV*L*FVRISLKPSCLSGPATIAPTRILGFAFLDSLHLKPPLHP 309
Query: 516 **TPFLGIEAPRW*TCLG*RFPNPTT*LIFKGF 614
TP GI+AP *T LG FP PTT*LIF GF
Sbjct: 308 YLTPL*GIDAPL**TRLGYFFPKPTT*LIFCGF 210
>gb|AW983004.3|AW983004 HVSMEg0004M14f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0004M14f, mRNA sequence
Length = 562
Score = 165 bits (354), Expect = 3e-040
Identities = 60/77 (77%), Positives = 68/77 (88%)
Frame = -3 / +1
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP KMSYVVGFGK+YP+ VHHRGASIP NGV YGCKGG+KWR++KK NPN + GAMVAGP
Sbjct: 262 NPQKMSYVVGFGKKYPKRVHHRGASIPHNGVKYGCKGGFKWRESKKANPNILVGAMVAGP 441
Query: 433 DRHDGFKDARRNYNYTE 383
D+HDGFKD R NYNYT+
Sbjct: 442 DKHDGFKDIRTNYNYTK 492
Score = 116 bits (249), Expect = 8e-026
Identities = 58/93 (62%), Positives = 62/93 (66%)
Frame = +3 / -3
Query: 336 KATNAATRPALPASVASV*L*FLRASLNPSWRSGPATMAPAMVLGFGFLASLHFHPPLQP 515
K N A RPA A V V*L*F+R SL PS SGPAT+AP +LGF FL SLH PPL P
Sbjct: 539 KEINVAPRPAFAARVGFV*L*FVRISLKPSCLSGPATIAPTRILGFAFLDSLHLKPPLHP 360
Query: 516 **TPFLGIEAPRW*TCLG*RFPNPTT*LIFKGF 614
TP GI+AP *T LG FP PTT*LIF GF
Sbjct: 359 YLTPL*GIDAPL**TRLGYFFPKPTT*LIFCGF 261
>gb|AV916435.1|AV916435 AV916435 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags17d08 5', mRNA sequence
Length = 607
Score = 164 bits (353), Expect = 4e-040
Identities = 60/76 (78%), Positives = 67/76 (88%)
Frame = -3 / +3
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP KMSYVVGFGK+YP+ VHHRGASIP NGV YGCKGG+KWR++KK NPN + GAMVAGP
Sbjct: 378 NPQKMSYVVGFGKKYPKRVHHRGASIPHNGVKYGCKGGFKWRESKKANPNILVGAMVAGP 557
Query: 433 DRHDGFKDARRNYNYT 386
D+HDGFKD R NYNYT
Sbjct: 558 DKHDGFKDIRTNYNYT 605
Score = 104 bits (221), Expect = 6e-022
Identities = 51/77 (66%), Positives = 55/77 (71%)
Frame = +3 / -1
Query: 384 SV*L*FLRASLNPSWRSGPATMAPAMVLGFGFLASLHFHPPLQP**TPFLGIEAPRW*TC 563
SV*L*F+R SL PS SGPAT+AP +LGF FL SLH PPL P TP GI+AP *T
Sbjct: 607 SV*L*FVRISLKPSCLSGPATIAPTRILGFAFLDSLHLKPPLHPYLTPL*GIDAPL**TR 428
Query: 564 LG*RFPNPTT*LIFKGF 614
LG FP PTT*LIF GF
Sbjct: 427 LGYFFPKPTT*LIFCGF 377
>gb|CB859220.1|CB859220 HI09M02w HI Hordeum vulgare subsp. vulgare cDNA clone HI09M02
3-PRIME, mRNA sequence
Length = 649
Score = 133 bits (286), Expect(2) = 6e-037
Identities = 48/76 (63%), Positives = 61/76 (80%)
Frame = -3 / -2
Query: 610 PLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGPD 431
P+KMSYVVG+GK+YP+ +HHRGAS PKNG+ Y C GG KWRD+KK +P+ + GAMV GPD
Sbjct: 648 PMKMSYVVGYGKKYPKRLHHRGASTPKNGIKYSCTGGNKWRDSKKADPHVLTGAMVGGPD 469
Query: 430 RHDGFKDARRNYNYTE 383
++D FKDAR +Y E
Sbjct: 468 KNDKFKDARISYAQNE 421
Score = 101 bits (216), Expect(2) = 2e-029
Identities = 52/98 (53%), Positives = 59/98 (60%)
Frame = +3 / +2
Query: 318 PWPSPDKATNAATRPALPASVASV*L*FLRASLNPSWRSGPATMAPAMVLGFGFLASLHF 497
P P AT+AAT PALP SV S * + ASLN S+ SGP TMAP G FL S H
Sbjct: 356 PRPLLVMATSAATSPALPTSVGSFCA*LILASLNLSFLSGPPTMAPVST*GSAFLESRHL 535
Query: 498 HPPLQP**TPFLGIEAPRW*TCLG*RFPNPTT*LIFKG 611
PP+ PFLG++APRW + G P PTT*LIF G
Sbjct: 536 LPPVHEYLIPFLGVDAPRWCSRFGYFLPYPTT*LIFIG 649
Score = 46.4 bits (95), Expect(2) = 2e-029
Identities = 18/30 (60%), Positives = 20/30 (66%)
Frame = +1 / +3
Query: 232 VTVSMEPAAAEAKGTWAVLQRTWCSCPPRR 321
+T S PAAA GTWA +RTWCSC RR
Sbjct: 258 LTASTWPAAAGRPGTWAARRRTWCSCRRRR 347
Score = 40.0 bits (81), Expect(2) = 6e-037
Identities = 18/29 (62%), Positives = 19/29 (65%)
Frame = -2 / -1
Query: 320 RRGGQEHHVLCSTAHVPFASAAAGSMETV 234
RR QEHHVL AHVP AAAG +E V
Sbjct: 346 RRRRQEHHVLRRAAHVPGRPAAAGHVEAV 260
>gb|BU994975.1|BU994975 HM08M21r HM Hordeum vulgare subsp. vulgare cDNA clone HM08M21
5-PRIME, mRNA sequence
Length = 537
Score = 136 bits (292), Expect(2) = 9e-035
Identities = 49/77 (63%), Positives = 62/77 (80%)
Frame = -3 / +2
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP+KMSYVVG+GK+YP+ +HHRGAS PKNG+ Y C GG KWRD+KK +P+ + GAMV GP
Sbjct: 173 NPMKMSYVVGYGKKYPKRLHHRGASTPKNGIKYSCTGGNKWRDSKKADPHVLTGAMVGGP 352
Query: 433 DRHDGFKDARRNYNYTE 383
D++D FKDAR +Y E
Sbjct: 353 DKNDKFKDARISYAQNE 403
Score = 101 bits (216), Expect(2) = 2e-026
Identities = 52/98 (53%), Positives = 59/98 (60%)
Frame = +3 / -1
Query: 318 PWPSPDKATNAATRPALPASVASV*L*FLRASLNPSWRSGPATMAPAMVLGFGFLASLHF 497
P P AT+AAT PALP SV S * + ASLN S+ SGP TMAP G FL S H
Sbjct: 468 PRPLLVMATSAATSPALPTSVGSFCA*LILASLNLSFLSGPPTMAPVST*GSAFLESRHL 289
Query: 498 HPPLQP**TPFLGIEAPRW*TCLG*RFPNPTT*LIFKG 611
PP+ PFLG++APRW + G P PTT*LIF G
Sbjct: 288 LPPVHEYLIPFLGVDAPRWCSRFGYFLPYPTT*LIFIG 175
Score = 36.8 bits (74), Expect(2) = 2e-026
Identities = 12/17 (70%), Positives = 13/17 (76%)
Frame = +1 / -2
Query: 271 GTWAVLQRTWCSCPPRR 321
GTWA +RTWCSC RR
Sbjct: 527 GTWAARRRTWCSCRRRR 477
Score = 29.9 bits (59), Expect(2) = 9e-035
Identities = 12/17 (70%), Positives = 12/17 (70%)
Frame = -2 / +1
Query: 320 RRGGQEHHVLCSTAHVP 270
RR QEHHVL AHVP
Sbjct: 478 RRRRQEHHVLRRAAHVP 528
>gb|AV923747.1|AV923747 AV923747 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd1e17 5', mRNA sequence
Length = 507
Score = 144 bits (308), Expect = 6e-034
Identities = 52/66 (78%), Positives = 59/66 (89%)
Frame = -3 / +1
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP KMSYVVGFGK+YP+ VHHRGASIP NGV YGCKGG+KWR++KK NPN + GAMVAGP
Sbjct: 310 NPQKMSYVVGFGKKYPKRVHHRGASIPHNGVKYGCKGGFKWRESKKANPNILVGAMVAGP 489
Query: 433 DRHDGF 416
D+HDGF
Sbjct: 490 DKHDGF 507
>gb|AV920035.1|AV920035 AV920035 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags9a10 3', mRNA sequence
Length = 664
Score = 140 bits (300), Expect(2) = 8e-034
Identities = 50/65 (76%), Positives = 57/65 (87%)
Frame = -3 / -1
Query: 577 KRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGPDRHDGFKDARRN 398
K+YP+ VHHRGASIP NGV YGCKGG+KWR++KK NPN + GAMVAGPD+HDGFKD R N
Sbjct: 664 KKYPKRVHHRGASIPHNGVKYGCKGGFKWRESKKANPNILVGAMVAGPDKHDGFKDIRTN 485
Query: 397 YNYTE 383
YNYTE
Sbjct: 484 YNYTE 470
Score = 102 bits (218), Expect = 1e-021
Identities = 50/78 (64%), Positives = 54/78 (69%)
Frame = +3 / +3
Query: 336 KATNAATRPALPASVASV*L*FLRASLNPSWRSGPATMAPAMVLGFGFLASLHFHPPLQP 515
K NAATRPA A V SV*L*F+R SL PS SGPAT+AP +LGF FL SLH PPL P
Sbjct: 423 KEINAATRPAFAARVGSV*L*FVRISLKPSCLSGPATIAPTRILGFAFLDSLHLKPPLHP 602
Query: 516 **TPFLGIEAPRW*TCLG 569
TP GI+AP *T LG
Sbjct: 603 YLTPL*GIDAPL**TRLG 656
Score = 23.1 bits (44), Expect(2) = 8e-034
Identities = 8/10 (80%), Positives = 10/10 (100%)
Frame = -3 / -1
Query: 313 VDKNTMFSAV 284
+DKNT+FSAV
Sbjct: 394 IDKNTIFSAV 365
>gb|AV917955.1|AV917955 AV917955 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags17d08 3', mRNA sequence
Length = 663
Score = 138 bits (295), Expect(2) = 4e-033
Identities = 49/64 (76%), Positives = 56/64 (87%)
Frame = -3 / -3
Query: 574 RYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGPDRHDGFKDARRNY 395
+YP+ VHHRGASIP NGV YGCKGG+KWR++KK NPN + GAMVAGPD+HDGFKD R NY
Sbjct: 661 KYPKRVHHRGASIPHNGVKYGCKGGFKWRESKKANPNILVGAMVAGPDKHDGFKDIRTNY 482
Query: 394 NYTE 383
NYTE
Sbjct: 481 NYTE 470
Score = 102 bits (218), Expect = 1e-021
Identities = 50/78 (64%), Positives = 54/78 (69%)
Frame = +3 / +3
Query: 336 KATNAATRPALPASVASV*L*FLRASLNPSWRSGPATMAPAMVLGFGFLASLHFHPPLQP 515
K NAATRPA A V SV*L*F+R SL PS SGPAT+AP +LGF FL SLH PPL P
Sbjct: 423 KEINAATRPAFAARVGSV*L*FVRISLKPSCLSGPATIAPTRILGFAFLDSLHLKPPLHP 602
Query: 516 **TPFLGIEAPRW*TCLG 569
TP GI+AP *T LG
Sbjct: 603 YLTPL*GIDAPL**TRLG 656
Score = 23.1 bits (44), Expect(2) = 4e-033
Identities = 8/10 (80%), Positives = 10/10 (100%)
Frame = -3 / -3
Query: 313 VDKNTMFSAV 284
+DKNT+FSAV
Sbjct: 394 IDKNTIFSAV 365
>gb|BJ456192.1|BJ456192 BJ456192 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak20b09 3', mRNA sequence
Length = 663
Score = 138 bits (295), Expect(2) = 4e-033
Identities = 49/64 (76%), Positives = 56/64 (87%)
Frame = -3 / -2
Query: 574 RYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGPDRHDGFKDARRNY 395
+YP+ VHHRGASIP NGV YGCKGG+KWR++KK NPN + GAMVAGPD+HDGFKD R NY
Sbjct: 662 KYPKRVHHRGASIPHNGVKYGCKGGFKWRESKKANPNILVGAMVAGPDKHDGFKDIRTNY 483
Query: 394 NYTE 383
NYTE
Sbjct: 482 NYTE 471
Score = 102 bits (218), Expect = 1e-021
Identities = 50/78 (64%), Positives = 54/78 (69%)
Frame = +3 / +1
Query: 336 KATNAATRPALPASVASV*L*FLRASLNPSWRSGPATMAPAMVLGFGFLASLHFHPPLQP 515
K NAATRPA A V SV*L*F+R SL PS SGPAT+AP +LGF FL SLH PPL P
Sbjct: 424 KEINAATRPAFAARVGSV*L*FVRISLKPSCLSGPATIAPTRILGFAFLDSLHLKPPLHP 603
Query: 516 **TPFLGIEAPRW*TCLG 569
TP GI+AP *T LG
Sbjct: 604 YLTPL*GIDAPL**TRLG 657
Score = 23.1 bits (44), Expect(2) = 4e-033
Identities = 8/10 (80%), Positives = 10/10 (100%)
Frame = -3 / -2
Query: 313 VDKNTMFSAV 284
+DKNT+FSAV
Sbjct: 395 IDKNTIFSAV 366
>gb|BU985662.1|BU985662 HF08E02r HF Hordeum vulgare subsp. vulgare cDNA clone HF08E02
5-PRIME, mRNA sequence
Length = 583
Score = 141 bits (302), Expect = 4e-033
Identities = 51/65 (78%), Positives = 58/65 (89%)
Frame = -3 / +3
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP KMSYVVGFGK+YP+ VHHRGASIP NGV YGCKGG+KWR++KK NPN + GAMVAGP
Sbjct: 387 NPQKMSYVVGFGKKYPKRVHHRGASIPHNGVKYGCKGGFKWRESKKANPNILVGAMVAGP 566
Query: 433 DRHDG 419
D+HDG
Sbjct: 567 DKHDG 581
>gb|BQ768653.1|BQ768653 EBro08_SQ012_F07_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ012_F07 5', mRNA sequence
Length = 408
Score = 136 bits (292), Expect = 9e-032
Identities = 49/77 (63%), Positives = 62/77 (80%)
Frame = -3 / +3
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP+KMSYVVG+GK+YP+ +HHRGAS PKNG+ Y C GG KWRD+KK +P+ + GAMV GP
Sbjct: 123 NPMKMSYVVGYGKKYPKRLHHRGASTPKNGIKYSCTGGNKWRDSKKADPHVLTGAMVGGP 302
Query: 433 DRHDGFKDARRNYNYTE 383
D++D FKDAR +Y E
Sbjct: 303 DKNDKFKDARISYAQNE 353
Score = 100 bits (214), Expect = 5e-021
Identities = 50/91 (54%), Positives = 57/91 (62%)
Frame = +3 / -3
Query: 339 ATNAATRPALPASVASV*L*FLRASLNPSWRSGPATMAPAMVLGFGFLASLHFHPPLQP* 518
AT+AAT PALP SV S * + ASLN S+ SGP TMAP G FL S H PP+
Sbjct: 397 ATSAATSPALPTSVGSFCA*LILASLNLSFLSGPPTMAPVST*GSAFLESRHLLPPVHEY 218
Query: 519 *TPFLGIEAPRW*TCLG*RFPNPTT*LIFKG 611
PFLG++APRW + G P PTT*LIF G
Sbjct: 217 LIPFLGVDAPRWCSRFGYFLPYPTT*LIFIG 125
>gb|BQ764603.1|BQ764603 EBca01_SQ004_I10_R carpel, pre-anthesis, no treatment, cv Optic,
EBca01 Hordeum vulgare subsp. vulgare cDNA clone
EBca01_SQ004_I10 5', mRNA sequence
Length = 512
Score = 115 bits (246), Expect(2) = 2e-026
Identities = 42/54 (77%), Positives = 47/54 (87%)
Frame = -3 / +1
Query: 544 ASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGPDRHDGFKDARRNYNYTE 383
ASIP NGV YGCKGG+KWR++KK NPN + GAMVAGPD+HDGFKD R NYNYTE
Sbjct: 1 ASIPHNGVKYGCKGGFKWRESKKANPNILVGAMVAGPDKHDGFKDIRTNYNYTE 162
Score = 23.1 bits (44), Expect(2) = 2e-026
Identities = 8/10 (80%), Positives = 10/10 (100%)
Frame = -3 / +1
Query: 313 VDKNTMFSAV 284
+DKNT+FSAV
Sbjct: 238 IDKNTIFSAV 267
>gb|AV912096.1|AV912096 AV912096 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak2l16 3', mRNA sequence
Length = 569
Score = 91.3 bits (193), Expect(3) = 1e-025
Identities = 33/45 (73%), Positives = 38/45 (84%)
Frame = -3 / -1
Query: 517 YGCKGGWKWRDAKKPNPNTIAGAMVAGPDRHDGFKDARRNYNYTE 383
YG KGG+KWR++KK N N + GAMVAGPD+HDGFKD R NYNYTE
Sbjct: 509 YGXKGGFKWRESKKANXNILVGAMVAGPDKHDGFKDIRTNYNYTE 375
Score = 39.1 bits (79), Expect(3) = 1e-025
Identities = 13/17 (76%), Positives = 15/17 (88%)
Frame = -3 / -3
Query: 574 RYPRHVHHRGASIPKNG 524
+YP+ VHHRGASIP NG
Sbjct: 567 KYPKRVHHRGASIPHNG 517
Score = 23.1 bits (44), Expect(3) = 1e-025
Identities = 8/10 (80%), Positives = 10/10 (100%)
Frame = -3 / -1
Query: 313 VDKNTMFSAV 284
+DKNT+FSAV
Sbjct: 299 IDKNTIFSAV 270
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 342,551,649
Number of Sequences: 312970
Number of extensions: 6688125
Number of successful extensions: 106284
Number of sequences better than 1.0e-020: 28
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6489756477
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)