TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3390529.2.1
(407 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF624193.2|BF624193 HVSMEa0012J04f Hordeum vulgare seedl... 130 4e-048
gb|BG300284.1|BG300284 HVSMEb0011P06f Hordeum vulgare seedl... 130 5e-048
gb|CA024561.1|CA024561 HZ49K12r HZ Hordeum vulgare subsp. v... 130 2e-047
gb|BF266997.3|BF266997 HV_CEa0016K10f Hordeum vulgare seedl... 130 1e-044
gb|AF474923.1| Hordeum vulgare subsp. vulgare chalcone isom... 76 3e-041
gb|BM375220.2|BM375220 EBem06_SQ002_L10_R embryo, 21 DPA, n... 81 2e-030
gb|BU995376.1|BU995376 HM10B01r HM Hordeum vulgare subsp. v... 116 4e-026
>gb|BF624193.2|BF624193 HVSMEa0012J04f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0012J04f, mRNA sequence
Length = 487
Score = 130 bits (279), Expect(2) = 4e-048
Identities = 53/64 (82%), Positives = 58/64 (90%)
Frame = +1 / +2
Query: 214 DAAVPALAKKWGGKTADELASDAAFFRDVVTGDFEKFTRVTMILPLTGEQYAEKVTENCV 393
DAAV ALA KW GK A +LASDAAFFRDVVTG+FEKFTRVTMILPLTG QY++KVTENCV
Sbjct: 179 DAAVSALAAKWAGKPAADLASDAAFFRDVVTGEFEKFTRVTMILPLTGAQYSDKVTENCV 358
Query: 394 AFWK 405
A+WK
Sbjct: 359 AYWK 370
Score = 102 bits (217), Expect(2) = 5e-036
Identities = 47/66 (71%), Positives = 49/66 (74%)
Frame = -1 / -2
Query: 407 AFQNATQFSVTFSAYCSPVSGRIIVTLVNFSKSPVTTSRKKXXXXXXXXAVLPPHFLASA 228
AFQ ATQFSVT S YC+PVSGRIIVTLVNFS SPVTTSRKK A LP H ASA
Sbjct: 372 AFQYATQFSVTLSEYCAPVSGRIIVTLVNFSNSPVTTSRKKAASEARSAAGLPAHLAASA 193
Query: 227 GTAASS 210
TAAS+
Sbjct: 192 ETAASA 175
Score = 79.0 bits (166), Expect(2) = 4e-048
Identities = 31/37 (83%), Positives = 35/37 (94%)
Frame = +1 / +2
Query: 103 ARPPGSAGSHFLGGAGVRGLEIGGNFIKFTAIGVYLE 213
ARPPGSA +HFL GAGVRG+EIGG+FIKFTAIGVYL+
Sbjct: 65 ARPPGSAHAHFLAGAGVRGMEIGGHFIKFTAIGVYLQ 175
Score = 67.5 bits (141), Expect(2) = 5e-036
Identities = 30/53 (56%), Positives = 32/53 (60%)
Frame = -3 / -1
Query: 213 LQVHADGRELDEVASDLEASHACXXXXXXXXXXXXXXXXXEDDAVDHHLRHGH 55
LQVHADGRELDEVA+DL +HAC EDDAVD LRHGH
Sbjct: 175 LQVHADGRELDEVAADLHPAHACAGEEVGVGGARRAGERREDDAVDLQLRHGH 17
>gb|BG300284.1|BG300284 HVSMEb0011P06f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0011P06f, mRNA sequence
Length = 536
Score = 130 bits (279), Expect(2) = 5e-048
Identities = 53/64 (82%), Positives = 58/64 (90%)
Frame = +1 / +2
Query: 214 DAAVPALAKKWGGKTADELASDAAFFRDVVTGDFEKFTRVTMILPLTGEQYAEKVTENCV 393
DAAV ALA KW GK A +LASDAAFFRDVVTG+FEKFTRVTMILPLTG QY++KVTENCV
Sbjct: 245 DAAVSALAAKWAGKPAADLASDAAFFRDVVTGEFEKFTRVTMILPLTGAQYSDKVTENCV 424
Query: 394 AFWK 405
A+WK
Sbjct: 425 AYWK 436
Score = 102 bits (217), Expect(2) = 5e-036
Identities = 47/66 (71%), Positives = 49/66 (74%)
Frame = -1 / -3
Query: 407 AFQNATQFSVTFSAYCSPVSGRIIVTLVNFSKSPVTTSRKKXXXXXXXXAVLPPHFLASA 228
AFQ ATQFSVT S YC+PVSGRIIVTLVNFS SPVTTSRKK A LP H ASA
Sbjct: 438 AFQYATQFSVTLSEYCAPVSGRIIVTLVNFSNSPVTTSRKKAASEARSAAGLPAHLAASA 259
Query: 227 GTAASS 210
TAAS+
Sbjct: 258 ETAASA 241
Score = 79.0 bits (166), Expect(2) = 5e-048
Identities = 31/37 (83%), Positives = 35/37 (94%)
Frame = +1 / +2
Query: 103 ARPPGSAGSHFLGGAGVRGLEIGGNFIKFTAIGVYLE 213
ARPPGSA +HFL GAGVRG+EIGG+FIKFTAIGVYL+
Sbjct: 131 ARPPGSAHAHFLAGAGVRGMEIGGHFIKFTAIGVYLQ 241
Score = 67.5 bits (141), Expect(2) = 5e-036
Identities = 30/53 (56%), Positives = 32/53 (60%)
Frame = -3 / -2
Query: 213 LQVHADGRELDEVASDLEASHACXXXXXXXXXXXXXXXXXEDDAVDHHLRHGH 55
LQVHADGRELDEVA+DL +HAC EDDAVD LRHGH
Sbjct: 241 LQVHADGRELDEVAADLHPAHACAGEEVGVGGARRAGERREDDAVDLQLRHGH 83
>gb|CA024561.1|CA024561 HZ49K12r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ49K12
5-PRIME, mRNA sequence
Length = 499
Score = 130 bits (279), Expect(2) = 2e-047
Identities = 53/64 (82%), Positives = 58/64 (90%)
Frame = +1 / +2
Query: 214 DAAVPALAKKWGGKTADELASDAAFFRDVVTGDFEKFTRVTMILPLTGEQYAEKVTENCV 393
DAAV ALA KW GK A +LASDAAFFRDVVTG+FEKFTRVTMILPLTG QY++KVTENCV
Sbjct: 110 DAAVSALAAKWAGKPAADLASDAAFFRDVVTGEFEKFTRVTMILPLTGAQYSDKVTENCV 289
Query: 394 AFWK 405
A+WK
Sbjct: 290 AYWK 301
Score = 102 bits (217), Expect(2) = 1e-030
Identities = 47/66 (71%), Positives = 49/66 (74%)
Frame = -1 / -2
Query: 407 AFQNATQFSVTFSAYCSPVSGRIIVTLVNFSKSPVTTSRKKXXXXXXXXAVLPPHFLASA 228
AFQ ATQFSVT S YC+PVSGRIIVTLVNFS SPVTTSRKK A LP H ASA
Sbjct: 303 AFQYATQFSVTLSEYCAPVSGRIIVTLVNFSNSPVTTSRKKAASEARSAAGLPAHLAASA 124
Query: 227 GTAASS 210
TAAS+
Sbjct: 123 ETAASA 106
Score = 77.1 bits (162), Expect(2) = 2e-047
Identities = 30/35 (85%), Positives = 33/35 (94%)
Frame = +1 / +2
Query: 109 PPGSAGSHFLGGAGVRGLEIGGNFIKFTAIGVYLE 213
PPGSA +HFL GAGVRG+EIGGNFIKFTAIGVYL+
Sbjct: 2 PPGSAHAHFLAGAGVRGMEIGGNFIKFTAIGVYLQ 106
Score = 49.6 bits (102), Expect(2) = 1e-030
Identities = 19/23 (82%), Positives = 21/23 (91%)
Frame = -3 / -1
Query: 213 LQVHADGRELDEVASDLEASHAC 145
LQVHADGRELDEVA+DL +HAC
Sbjct: 106 LQVHADGRELDEVAADLHPAHAC 38
>gb|BF266997.3|BF266997 HV_CEa0016K10f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0016K10f, mRNA sequence
Length = 751
Score = 130 bits (279), Expect(2) = 1e-044
Identities = 53/64 (82%), Positives = 58/64 (90%)
Frame = +1 / +3
Query: 214 DAAVPALAKKWGGKTADELASDAAFFRDVVTGDFEKFTRVTMILPLTGEQYAEKVTENCV 393
DAAV ALA KW GK A +LASDAAFFRDVVTG+FEKFTRVTMILPLTG QY++KVTENCV
Sbjct: 105 DAAVSALAAKWAGKPAADLASDAAFFRDVVTGEFEKFTRVTMILPLTGAQYSDKVTENCV 284
Query: 394 AFWK 405
A+WK
Sbjct: 285 AYWK 296
Score = 102 bits (217), Expect(2) = 2e-030
Identities = 47/66 (71%), Positives = 49/66 (74%)
Frame = -1 / -1
Query: 407 AFQNATQFSVTFSAYCSPVSGRIIVTLVNFSKSPVTTSRKKXXXXXXXXAVLPPHFLASA 228
AFQ ATQFSVT S YC+PVSGRIIVTLVNFS SPVTTSRKK A LP H ASA
Sbjct: 298 AFQYATQFSVTLSEYCAPVSGRIIVTLVNFSNSPVTTSRKKAASEARSAAGLPAHLAASA 119
Query: 227 GTAASS 210
TAAS+
Sbjct: 118 ETAASA 101
Score = 68.4 bits (143), Expect(2) = 1e-044
Identities = 27/33 (81%), Positives = 31/33 (93%)
Frame = +1 / +3
Query: 115 GSAGSHFLGGAGVRGLEIGGNFIKFTAIGVYLE 213
GSA +HFL GAGVRG+EIGG+FIKFTAIGVYL+
Sbjct: 3 GSAHAHFLAGAGVRGMEIGGHFIKFTAIGVYLQ 101
Score = 49.6 bits (102), Expect(2) = 2e-030
Identities = 19/23 (82%), Positives = 21/23 (91%)
Frame = -3 / -3
Query: 213 LQVHADGRELDEVASDLEASHAC 145
LQVHADGRELDEVA+DL +HAC
Sbjct: 101 LQVHADGRELDEVAADLHPAHAC 33
>gb|AF474923.1| Hordeum vulgare subsp. vulgare chalcone isomerase (CHI) gene,
complete cds
Length = 11475
Score = 75.8 bits (159), Expect(4) = 3e-041
Identities = 28/33 (84%), Positives = 32/33 (96%)
Frame = +1 / +3
Query: 307 GDFEKFTRVTMILPLTGEQYAEKVTENCVAFWK 405
G+FEKFTRVTMILPLTG QY++KVTENCVA+WK
Sbjct: 7392 GEFEKFTRVTMILPLTGAQYSDKVTENCVAYWK 7490
Score = 68.9 bits (144), Expect(3) = 2e-027
Identities = 29/34 (85%), Positives = 30/34 (88%)
Frame = -1 / -3
Query: 407 AFQNATQFSVTFSAYCSPVSGRIIVTLVNFSKSP 306
AFQ ATQFSVT S YC+PVSGRIIVTLVNFS SP
Sbjct: 7492 AFQYATQFSVTLSEYCAPVSGRIIVTLVNFSNSP 7391
Score = 61.1 bits (127), Expect(4) = 3e-041
Identities = 26/33 (78%), Positives = 28/33 (84%)
Frame = +1 / +1
Query: 214 DAAVPALAKKWGGKTADELASDAAFFRDVVTGD 312
DAAV ALA KW GK A +LASDAAFFRDVVTG+
Sbjct: 7219 DAAVSALAAKWAGKPAADLASDAAFFRDVVTGN 7317
Score = 51.5 bits (106), Expect(4) = 3e-041
Identities = 20/23 (86%), Positives = 23/23 (100%)
Frame = +1 / +1
Query: 145 AGVRGLEIGGNFIKFTAIGVYLE 213
AGVRG+EIGG+FIKFTAIGVYL+
Sbjct: 7147 AGVRGMEIGGHFIKFTAIGVYLQ 7215
Score = 49.6 bits (102), Expect(3) = 2e-027
Identities = 19/23 (82%), Positives = 21/23 (91%)
Frame = -3 / -1
Query: 213 LQVHADGRELDEVASDLEASHAC 145
LQVHADGRELDEVA+DL +HAC
Sbjct: 7215 LQVHADGRELDEVAADLHPAHAC 7147
Score = 40.9 bits (83), Expect(3) = 2e-027
Identities = 20/35 (57%), Positives = 21/35 (60%)
Frame = -1 / -2
Query: 314 KSPVTTSRKKXXXXXXXXAVLPPHFLASAGTAASS 210
K PVTTSRKK A LP H ASA TAAS+
Sbjct: 7319 KLPVTTSRKKAASEARSAAGLPAHLAASAETAASA 7215
Score = 35.0 bits (70), Expect(4) = 3e-041
Identities = 13/16 (81%), Positives = 14/16 (87%)
Frame = +1 / +1
Query: 103 ARPPGSAGSHFLGGAG 150
ARPPGSA +HFL GAG
Sbjct: 6985 ARPPGSAHAHFLAGAG 7032
>gb|BM375220.2|BM375220 EBem06_SQ002_L10_R embryo, 21 DPA, no treatment, cv Optic, EBem06
Hordeum vulgare subsp. vulgare cDNA clone
EBem06_SQ002_L10 5', mRNA sequence
Length = 375
Score = 81.3 bits (171), Expect(2) = 2e-030
Identities = 32/37 (86%), Positives = 35/37 (94%)
Frame = +1 / +2
Query: 103 ARPPGSAGSHFLGGAGVRGLEIGGNFIKFTAIGVYLE 213
ARPPGSA +HFL GAGVRG+EIGGNFIKFTAIGVYL+
Sbjct: 152 ARPPGSAHAHFLAGAGVRGMEIGGNFIKFTAIGVYLQ 262
Score = 68.9 bits (144), Expect(2) = 2e-030
Identities = 29/36 (80%), Positives = 31/36 (86%)
Frame = +1 / +2
Query: 214 DAAVPALAKKWGGKTADELASDAAFFRDVVTGDFEK 321
DAAV ALA KW GK A +LASDAAFFRDVVTG+FEK
Sbjct: 266 DAAVSALAAKWAGKPAADLASDAAFFRDVVTGEFEK 373
>gb|BU995376.1|BU995376 HM10B01r HM Hordeum vulgare subsp. vulgare cDNA clone HM10B01
5-PRIME, mRNA sequence
Length = 602
Score = 116 bits (249), Expect = 4e-026
Identities = 45/56 (80%), Positives = 52/56 (92%)
Frame = +1 / +3
Query: 238 KKWGGKTADELASDAAFFRDVVTGDFEKFTRVTMILPLTGEQYAEKVTENCVAFWK 405
++W GK A +LASDAAFFRDVVTG+FEKFTRVTMILPLTG QY++KVTENCVA+WK
Sbjct: 33 RQWAGKPAADLASDAAFFRDVVTGEFEKFTRVTMILPLTGAQYSDKVTENCVAYWK 200
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 116,401,485
Number of Sequences: 312970
Number of extensions: 1122604
Number of successful extensions: 77222
Number of sequences better than 1.0e-020: 9
length of database: 58,378,179
effective HSP length: 49
effective length of database: 43,042,649
effective search space used: 3701667814
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)