TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3204486.2.1
(607 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ763635.1|BQ763635 EBro03_SQ005_B06_R root, 3 week, wat... 193 7e-055
gb|CA013018.1|CA013018 HT07C12r HT Hordeum vulgare subsp. v... 193 7e-055
gb|AF023472.1|AF023472 Hordeum vulgare peptide transporter ... 193 2e-054
gb|AJ435519.1|AJ435519 AJ435519 S00002 Hordeum vulgare subs... 168 2e-047
gb|BM100095.2|BM100095 EBma01_SQ001_C23_R maternal, 4 DPA, ... 187 6e-047
gb|BF259472.2|BF259472 HVSMEf0019D07f Hordeum vulgare seedl... 169 1e-046
gb|AV925465.1|AV925465 AV925465 K. Sato unpublished cDNA li... 146 8e-035
gb|AV930611.1|AV930611 AV930611 K. Sato unpublished cDNA li... 146 8e-035
gb|AV931970.1|AV931970 AV931970 K. Sato unpublished cDNA li... 146 8e-035
gb|BQ659812.1|BQ659812 HF01M18w HF Hordeum vulgare subsp. v... 127 7e-029
gb|AV834230.1|AV834230 AV834230 K. Sato unpublished cDNA li... 111 3e-024
gb|AJ435085.1|AJ435085 AJ435085 S00002 Hordeum vulgare subs... 111 3e-024
gb|BQ134693.1|BQ134693 LP30190 Lemma/palea-enriched cDNA li... 105 2e-022
gb|BJ466430.1|BJ466430 BJ466430 K. Sato unpublished cDNA li... 104 4e-022
gb|BJ468216.1|BJ468216 BJ468216 K. Sato unpublished cDNA li... 104 4e-022
gb|BJ458380.1|BJ458380 BJ458380 K. Sato unpublished cDNA li... 104 6e-022
gb|BU993945.1|BU993945 HM05A23r HM Hordeum vulgare subsp. v... 102 2e-021
gb|BG301085.2|BG301085 HVSMEb0019I02f Hordeum vulgare seedl... 100 1e-020
>gb|BQ763635.1|BQ763635 EBro03_SQ005_B06_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ005_B06 5', mRNA sequence
Length = 704
Score = 193 bits (416), Expect(2) = 7e-055
Identities = 78/101 (77%), Positives = 83/101 (82%)
Frame = +3 / +2
Query: 3 RAIARHGLYGEDDIVPISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALS 182
R IA+HGLYGE D+VPISI WQ+PQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALS
Sbjct: 44 RTIAQHGLYGEKDVVPISIFWQVPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALS 223
Query: 183 LTTVALGNYLSXXXXXXXXXXXXRHGRIGWIPENLNRGHLD 305
LTTVALGNYLS R G+ GWIP+NLN GHLD
Sbjct: 224 LTTVALGNYLSTLLVTVVAKVTTRGGKQGWIPDNLNVGHLD 346
Score = 143 bits (306), Expect(2) = 3e-054
Identities = 59/74 (79%), Positives = 64/74 (86%)
Frame = -3 / -2
Query: 224 QDRAEVVAERDGGERQRGAHAPHGVGRLVVEELQLPHEGEHLRAPDDEVLRYLPADGDGH 45
+ RAEVV ER GGER+RGAHAPHGVGRLVVEEL+LPHEGEHL A DDEVLR+LP DGDGH
Sbjct: 265 EQRAEVVPERHGGERERGAHAPHGVGRLVVEELELPHEGEHLGAADDEVLRHLPEDGDGH 86
Query: 44 DVVLAVQAVPRDGA 3
V+L VQ V RDGA
Sbjct: 85 HVLLPVQPVLRDGA 44
Score = 142 bits (304), Expect(2) = 2e-042
Identities = 58/67 (86%), Positives = 60/67 (89%)
Frame = -1 / -3
Query: 202 PSATVVSDSAEHMLLMASGAWS*KNSSCPTKVNTSAHPMMKYCGICQQMEMGTMSSSPYR 23
PSATVVS+SAEHMLLMASGAWS*KNSSCPTKVNTSA PMMKYCG CQ+MEMGT S SPY
Sbjct: 243 PSATVVSESAEHMLLMASGAWS*KNSSCPTKVNTSAQPMMKYCGTCQKMEMGTTSFSPYS 64
Query: 22 PCRAMAR 2
PC AM R
Sbjct: 63 PCCAMVR 43
Score = 88.6 bits (187), Expect(2) = 3e-054
Identities = 41/54 (75%), Positives = 43/54 (79%)
Frame = -1 / -3
Query: 418 PLAPG*SSAVFLYLYQLAMTR*ARKLRRLSTASSQKK*SRWPRLRFSGIHPMRP 257
PLA G SSAVFL +YQLAM+R* KL RLS ASSQKK*SRWP LR SGIHP P
Sbjct: 459 PLASGESSAVFL*VYQLAMSR*TAKLTRLSAASSQKK*SRWPTLRLSGIHPCLP 298
Score = 50.1 bits (103), Expect(2) = 2e-042
Identities = 22/34 (64%), Positives = 26/34 (76%)
Frame = -3 / -2
Query: 416 FGARVIIGRLLVLVPAGDDEVGKEVEKAEHGEQP 315
FG R I G LLV VPAGD++V EV++AE GEQP
Sbjct: 457 FGVRRIFGGLLVGVPAGDEQVDGEVDEAECGEQP 356
Score = 40.5 bits (82), Expect(2) = 7e-055
Identities = 15/19 (78%), Positives = 16/19 (84%)
Frame = +3 / +2
Query: 363 IASWYKYKKTADDYPGAKG 419
IASWY YKKTA+D P AKG
Sbjct: 404 IASWYTYKKTAEDSPDAKG 460
>gb|CA013018.1|CA013018 HT07C12r HT Hordeum vulgare subsp. vulgare cDNA clone HT07C12
5-PRIME, mRNA sequence
Length = 623
Score = 193 bits (416), Expect(2) = 7e-055
Identities = 78/101 (77%), Positives = 83/101 (82%)
Frame = +3 / +2
Query: 3 RAIARHGLYGEDDIVPISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALS 182
R IA+HGLYGE D+VPISI WQ+PQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALS
Sbjct: 179 RTIAQHGLYGEKDVVPISIFWQVPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALS 358
Query: 183 LTTVALGNYLSXXXXXXXXXXXXRHGRIGWIPENLNRGHLD 305
LTTVALGNYLS R G+ GWIP+NLN GHLD
Sbjct: 359 LTTVALGNYLSTLLVTVVAKVTTRGGKQGWIPDNLNVGHLD 481
Score = 143 bits (306), Expect(2) = 3e-054
Identities = 59/74 (79%), Positives = 64/74 (86%)
Frame = -3 / -2
Query: 224 QDRAEVVAERDGGERQRGAHAPHGVGRLVVEELQLPHEGEHLRAPDDEVLRYLPADGDGH 45
+ RAEVV ER GGER+RGAHAPHGVGRLVVEEL+LPHEGEHL A DDEVLR+LP DGDGH
Sbjct: 400 EQRAEVVPERHGGERERGAHAPHGVGRLVVEELELPHEGEHLGAADDEVLRHLPEDGDGH 221
Query: 44 DVVLAVQAVPRDGA 3
V+L VQ V RDGA
Sbjct: 220 HVLLPVQPVLRDGA 179
Score = 142 bits (304), Expect(2) = 2e-042
Identities = 58/67 (86%), Positives = 60/67 (89%)
Frame = -1 / -3
Query: 202 PSATVVSDSAEHMLLMASGAWS*KNSSCPTKVNTSAHPMMKYCGICQQMEMGTMSSSPYR 23
PSATVVS+SAEHMLLMASGAWS*KNSSCPTKVNTSA PMMKYCG CQ+MEMGT S SPY
Sbjct: 378 PSATVVSESAEHMLLMASGAWS*KNSSCPTKVNTSAQPMMKYCGTCQKMEMGTTSFSPYS 199
Query: 22 PCRAMAR 2
PC AM R
Sbjct: 198 PCCAMVR 178
Score = 88.6 bits (187), Expect(2) = 3e-054
Identities = 41/54 (75%), Positives = 43/54 (79%)
Frame = -1 / -3
Query: 418 PLAPG*SSAVFLYLYQLAMTR*ARKLRRLSTASSQKK*SRWPRLRFSGIHPMRP 257
PLA G SSAVFL +YQLAM+R* KL RLS ASSQKK*SRWP LR SGIHP P
Sbjct: 594 PLASGESSAVFL*VYQLAMSR*TAKLTRLSAASSQKK*SRWPTLRLSGIHPCLP 433
Score = 50.1 bits (103), Expect(2) = 2e-042
Identities = 22/34 (64%), Positives = 26/34 (76%)
Frame = -3 / -2
Query: 416 FGARVIIGRLLVLVPAGDDEVGKEVEKAEHGEQP 315
FG R I G LLV VPAGD++V EV++AE GEQP
Sbjct: 592 FGVRRIFGGLLVGVPAGDEQVDGEVDEAECGEQP 491
Score = 40.5 bits (82), Expect(2) = 7e-055
Identities = 15/19 (78%), Positives = 16/19 (84%)
Frame = +3 / +2
Query: 363 IASWYKYKKTADDYPGAKG 419
IASWY YKKTA+D P AKG
Sbjct: 539 IASWYTYKKTAEDSPDAKG 595
>gb|AF023472.1|AF023472 Hordeum vulgare peptide transporter (ptr1) mRNA, complete cds
Length = 2260
Score = 193 bits (416), Expect(2) = 2e-054
Identities = 78/101 (77%), Positives = 83/101 (82%)
Frame = +3 / +3
Query: 3 RAIARHGLYGEDDIVPISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALS 182
R IA+HGLYGE D+VPISI WQ+PQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALS
Sbjct: 1581 RTIAQHGLYGEKDVVPISIFWQVPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALS 1760
Query: 183 LTTVALGNYLSXXXXXXXXXXXXRHGRIGWIPENLNRGHLD 305
LTTVALGNYLS R G+ GWIP+NLN GHLD
Sbjct: 1761 LTTVALGNYLSTLLVTVVAKVTTRGGKQGWIPDNLNVGHLD 1883
Score = 143 bits (306), Expect(2) = 2e-053
Identities = 59/74 (79%), Positives = 64/74 (86%)
Frame = -3 / -3
Query: 224 QDRAEVVAERDGGERQRGAHAPHGVGRLVVEELQLPHEGEHLRAPDDEVLRYLPADGDGH 45
+ RAEVV ER GGER+RGAHAPHGVGRLVVEEL+LPHEGEHL A DDEVLR+LP DGDGH
Sbjct: 1802 EQRAEVVPERHGGERERGAHAPHGVGRLVVEELELPHEGEHLGAADDEVLRHLPEDGDGH 1623
Query: 44 DVVLAVQAVPRDGA 3
V+L VQ V RDGA
Sbjct: 1622 HVLLPVQPVLRDGA 1581
Score = 142 bits (304), Expect(2) = 1e-042
Identities = 58/67 (86%), Positives = 60/67 (89%)
Frame = -1 / -1
Query: 202 PSATVVSDSAEHMLLMASGAWS*KNSSCPTKVNTSAHPMMKYCGICQQMEMGTMSSSPYR 23
PSATVVS+SAEHMLLMASGAWS*KNSSCPTKVNTSA PMMKYCG CQ+MEMGT S SPY
Sbjct: 1780 PSATVVSESAEHMLLMASGAWS*KNSSCPTKVNTSAQPMMKYCGTCQKMEMGTTSFSPYS 1601
Query: 22 PCRAMAR 2
PC AM R
Sbjct: 1600 PCCAMVR 1580
Score = 86.3 bits (182), Expect(2) = 2e-053
Identities = 40/54 (74%), Positives = 42/54 (77%)
Frame = -1 / -1
Query: 418 PLAPG*SSAVFLYLYQLAMTR*ARKLRRLSTASSQKK*SRWPRLRFSGIHPMRP 257
PLA G S AVFL +YQLAM+R* KL RLS ASSQKK*SRWP LR SGIHP P
Sbjct: 1996 PLASGESPAVFL*VYQLAMSR*TAKLTRLSAASSQKK*SRWPTLRLSGIHPCLP 1835
Score = 50.6 bits (104), Expect(2) = 1e-042
Identities = 22/34 (64%), Positives = 26/34 (76%)
Frame = -3 / -3
Query: 416 FGARVIIGRLLVLVPAGDDEVGKEVEKAEHGEQP 315
FG R I G LLV VPAGD++V EV++AE GEQP
Sbjct: 1994 FGVRRISGGLLVGVPAGDEQVDGEVDEAERGEQP 1893
Score = 39.1 bits (79), Expect(2) = 2e-054
Identities = 15/19 (78%), Positives = 15/19 (78%)
Frame = +3 / +3
Query: 363 IASWYKYKKTADDYPGAKG 419
IASWY YKKTA D P AKG
Sbjct: 1941 IASWYTYKKTAGDSPDAKG 1997
>gb|AJ435519.1|AJ435519 AJ435519 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200061H10F1, mRNA sequence
Length = 555
Score = 168 bits (361), Expect(2) = 2e-047
Identities = 68/88 (77%), Positives = 72/88 (81%)
Frame = +3 / +2
Query: 42 IVPISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLSXX 221
+VPISI WQ+PQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLS
Sbjct: 5 VVPISIFWQVPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLSTL 184
Query: 222 XXXXXXXXXXRHGRIGWIPENLNRGHLD 305
R G+ GWIP+NLN GHLD
Sbjct: 185 LVTVVAKVTTRGGKQGWIPDNLNVGHLD 268
Score = 126 bits (269), Expect(2) = 4e-047
Identities = 50/60 (83%), Positives = 54/60 (90%)
Frame = -3 / -3
Query: 224 QDRAEVVAERDGGERQRGAHAPHGVGRLVVEELQLPHEGEHLRAPDDEVLRYLPADGDGH 45
+ RAEVV ER GGER+RGAHAPHGVGRLVVEEL+LPHEGEHL A DDEVLR+LP DGDGH
Sbjct: 187 EQRAEVVPERHGGERERGAHAPHGVGRLVVEELELPHEGEHLGAADDEVLRHLPEDGDGH 8
Score = 121 bits (259), Expect(2) = 1e-036
Identities = 49/53 (92%), Positives = 51/53 (96%)
Frame = -1 / -1
Query: 202 PSATVVSDSAEHMLLMASGAWS*KNSSCPTKVNTSAHPMMKYCGICQQMEMGT 44
PSATVVS+SAEHMLLMASGAWS*KNSSCPTKVNTSA PMMKYCG CQ+MEMGT
Sbjct: 165 PSATVVSESAEHMLLMASGAWS*KNSSCPTKVNTSAQPMMKYCGTCQKMEMGT 7
Score = 81.7 bits (172), Expect(2) = 4e-047
Identities = 40/54 (74%), Positives = 42/54 (77%)
Frame = -1 / -1
Query: 418 PLAPG*SSAVFLYLYQLAMTR*ARKLRRLSTASSQKK*SRWPRLRFSGIHPMRP 257
PLA G SSAVFL +YQLAM+R* KL RLS ASSQKK*SR P LR SGIHP P
Sbjct: 381 PLASGESSAVFL*VYQLAMSR*TAKLTRLSAASSQKK*SR*PTLRLSGIHPCLP 220
Score = 51.5 bits (106), Expect(2) = 1e-036
Identities = 22/34 (64%), Positives = 26/34 (76%)
Frame = -3 / -3
Query: 416 FGARVIIGRLLVLVPAGDDEVGKEVEKAEHGEQP 315
FG R I G LLV VPAGD++V EV++AE GEQP
Sbjct: 379 FGVRRIFGGLLVGVPAGDEQVDGEVDEAERGEQP 278
Score = 40.5 bits (82), Expect(2) = 2e-047
Identities = 15/19 (78%), Positives = 16/19 (84%)
Frame = +3 / +2
Query: 363 IASWYKYKKTADDYPGAKG 419
IASWY YKKTA+D P AKG
Sbjct: 326 IASWYTYKKTAEDSPDAKG 382
>gb|BM100095.2|BM100095 EBma01_SQ001_C23_R maternal, 4 DPA, no treatment, cv Optic, EBma01
Hordeum vulgare subsp. vulgare cDNA clone
EBma01_SQ001_C23 5', mRNA sequence
Length = 412
Score = 187 bits (402), Expect = 6e-047
Identities = 76/101 (75%), Positives = 81/101 (80%)
Frame = +3 / +2
Query: 3 RAIARHGLYGEDDIVPISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALS 182
R IA+HGLYGE D+VPISI WQ+PQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALS
Sbjct: 92 RTIAQHGLYGEKDVVPISIFWQVPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALS 271
Query: 183 LTTVALGNYLSXXXXXXXXXXXXRHGRIGWIPENLNRGHLD 305
LTTVALGNYLS G+ GWIP+NLN HLD
Sbjct: 272 LTTVALGNYLSTLLVTVVAKVTTTGGKQGWIPDNLNVRHLD 394
Score = 143 bits (306), Expect(2) = 2e-039
Identities = 59/74 (79%), Positives = 64/74 (86%)
Frame = -3 / -1
Query: 224 QDRAEVVAERDGGERQRGAHAPHGVGRLVVEELQLPHEGEHLRAPDDEVLRYLPADGDGH 45
+ RAEVV ER GGER+RGAHAPHGVGRLVVEEL+LPHEGEHL A DDEVLR+LP DGDGH
Sbjct: 313 EQRAEVVPERHGGERERGAHAPHGVGRLVVEELELPHEGEHLGAADDEVLRHLPEDGDGH 134
Query: 44 DVVLAVQAVPRDGA 3
V+L VQ V RDGA
Sbjct: 133 HVLLPVQPVLRDGA 92
Score = 142 bits (304), Expect = 2e-033
Identities = 58/67 (86%), Positives = 60/67 (89%)
Frame = -1 / -2
Query: 202 PSATVVSDSAEHMLLMASGAWS*KNSSCPTKVNTSAHPMMKYCGICQQMEMGTMSSSPYR 23
PSATVVS+SAEHMLLMASGAWS*KNSSCPTKVNTSA PMMKYCG CQ+MEMGT S SPY
Sbjct: 291 PSATVVSESAEHMLLMASGAWS*KNSSCPTKVNTSAQPMMKYCGTCQKMEMGTTSFSPYS 112
Query: 22 PCRAMAR 2
PC AM R
Sbjct: 111 PCCAMVR 91
Score = 39.1 bits (79), Expect(2) = 2e-039
Identities = 16/21 (76%), Positives = 16/21 (76%)
Frame = -1 / -2
Query: 319 SQKK*SRWPRLRFSGIHPMRP 257
SQKK*SRW LR SGIHP P
Sbjct: 408 SQKK*SRWRTLRLSGIHPCLP 346
>gb|BF259472.2|BF259472 HVSMEf0019D07f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0019D07f, mRNA sequence
Length = 757
Score = 169 bits (363), Expect(2) = 1e-046
Identities = 68/101 (67%), Positives = 74/101 (73%)
Frame = +3 / +3
Query: 3 RAIARHGLYGEDDIVPISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALS 182
R IA+HGLYGE D+VPISI WQ+PQYFII C EVFTF GQLE+ YDQAPDAMRSMC LS
Sbjct: 282 RTIAQHGLYGEKDVVPISIFWQVPQYFIISCTEVFTFSGQLEYLYDQAPDAMRSMCCELS 461
Query: 183 LTTVALGNYLSXXXXXXXXXXXXRHGRIGWIPENLNRGHLD 305
LTTVALGNYL R G+ GWIP +LN GH D
Sbjct: 462 LTTVALGNYLRTLLVTEVAKVTTRGGKQGWIPGDLNVGHFD 584
Score = 124 bits (265), Expect(2) = 4e-044
Identities = 51/67 (76%), Positives = 55/67 (82%)
Frame = -1 / -1
Query: 202 PSATVVSDSAEHMLLMASGAWS*KNSSCPTKVNTSAHPMMKYCGICQQMEMGTMSSSPYR 23
PSATVVS+S++HMLLMASGAWS K SSCP KVNTS MMKYCG CQ+MEMGT S SPY
Sbjct: 481 PSATVVSESSQHMLLMASGAWSYKYSSCPEKVNTSVQLMMKYCGTCQKMEMGTTSFSPYS 302
Query: 22 PCRAMAR 2
PC AM R
Sbjct: 301 PCCAMVR 281
Score = 121 bits (258), Expect(2) = 1e-033
Identities = 53/74 (71%), Positives = 57/74 (77%)
Frame = -3 / -3
Query: 224 QDRAEVVAERDGGERQRGAHAPHGVGRLVVEELQLPHEGEHLRAPDDEVLRYLPADGDGH 45
+ R EVV ER GGER+ AHAPHGVGRLVV L LP EGEHL A DDEVLR+LP DGDGH
Sbjct: 503 EQRPEVVPERHGGERELAAHAPHGVGRLVV*VL*LPREGEHLGAADDEVLRHLPEDGDGH 324
Query: 44 DVVLAVQAVPRDGA 3
V+L VQ V RDGA
Sbjct: 323 HVLLPVQPVLRDGA 282
Score = 73.5 bits (154), Expect(2) = 4e-044
Identities = 35/53 (66%), Positives = 42/53 (79%)
Frame = -1 / -1
Query: 415 LAPG*SSAVFLYLYQLAMTR*ARKLRRLSTASSQKK*SRWPRLRFSGIHPMRP 257
LAPG S AVFL +YQLAM+R* +KL RLS+++SQKK*S+ P LR GIHP P
Sbjct: 694 LAPGESLAVFL*VYQLAMSR*TQKLTRLSSSTSQKK*SK*PTLRSPGIHPCLP 536
Score = 41.4 bits (84), Expect(2) = 1e-033
Identities = 19/35 (54%), Positives = 24/35 (68%)
Frame = -3 / -3
Query: 419 PFGARVIIGRLLVLVPAGDDEVGKEVEKAEHGEQP 315
P GAR I G LLV VPAG ++V EV++ E +QP
Sbjct: 698 PSGARRIFGGLLVGVPAGHEQVDAEVDEVELVDQP 594
Score = 36.8 bits (74), Expect(2) = 1e-046
Identities = 13/18 (72%), Positives = 15/18 (83%)
Frame = +3 / +3
Query: 363 IASWYKYKKTADDYPGAK 416
+ASWY YKKTA D PGA+
Sbjct: 642 MASWYTYKKTAKDSPGAR 695
>gb|AV925465.1|AV925465 AV925465 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd24f11 5', mRNA sequence
Length = 649
Score = 146 bits (314), Expect = 8e-035
Identities = 57/87 (65%), Positives = 65/87 (74%)
Frame = +3 / +3
Query: 45 VPISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLSXXX 224
VP+SI WQIPQYF++G +EVFTF+G LEFFYDQ+PDAMRS+CSAL L T ALGNYLS
Sbjct: 366 VPLSILWQIPQYFLVGASEVFTFIGALEFFYDQSPDAMRSLCSALQLITTALGNYLSAFI 545
Query: 225 XXXXXXXXXRHGRIGWIPENLNRGHLD 305
R GR GWIP+NLN G LD
Sbjct: 546 LTMVAYFTTRGGRPGWIPDNLNEGRLD 626
Score = 90.0 bits (190), Expect(2) = 2e-022
Identities = 42/66 (63%), Positives = 45/66 (68%)
Frame = -3 / -3
Query: 221 DRAEVVAERDGGERQRGAHAPHGVGRLVVEELQLPHEGEHLRAPDDEVLRYLPADGDGHD 42
+RAEVV ER G E QR A APHGV RLVVEEL+ P EGEHLR D EVLR LP D +
Sbjct: 542 ERAEVVPERRGDELQRAAQAPHGVRRLVVEELERPDEGEHLRGADQEVLRDLPQDAQWNR 363
Query: 41 VVLAVQ 24
VL Q
Sbjct: 362 DVLVHQ 345
Score = 35.4 bits (71), Expect(2) = 2e-022
Identities = 16/23 (69%), Positives = 16/23 (69%)
Frame = -1 / -1
Query: 325 ASSQKK*SRWPRLRFSGIHPMRP 257
ASSQKK SR P R SGIHP P
Sbjct: 646 ASSQKKLSRRPSFRLSGIHPGLP 578
>gb|AV930611.1|AV930611 AV930611 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd24f11 3', mRNA sequence
Length = 623
Score = 146 bits (314), Expect = 8e-035
Identities = 57/87 (65%), Positives = 65/87 (74%)
Frame = +3 / -1
Query: 45 VPISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLSXXX 224
VP+SI WQIPQYF++G +EVFTF+G LEFFYDQ+PDAMRS+CSAL L T ALGNYLS
Sbjct: 578 VPLSILWQIPQYFLVGASEVFTFIGALEFFYDQSPDAMRSLCSALQLITTALGNYLSAFI 399
Query: 225 XXXXXXXXXRHGRIGWIPENLNRGHLD 305
R GR GWIP+NLN G LD
Sbjct: 398 LTMVAYFTTRGGRPGWIPDNLNEGRLD 318
Score = 90.0 bits (190), Expect(2) = 6e-028
Identities = 42/66 (63%), Positives = 45/66 (68%)
Frame = -3 / +3
Query: 221 DRAEVVAERDGGERQRGAHAPHGVGRLVVEELQLPHEGEHLRAPDDEVLRYLPADGDGHD 42
+RAEVV ER G E QR A APHGV RLVVEEL+ P EGEHLR D EVLR LP D +
Sbjct: 402 ERAEVVPERRGDELQRAAQAPHGVRRLVVEELERPDEGEHLRGADQEVLRDLPQDAQWNR 581
Query: 41 VVLAVQ 24
VL Q
Sbjct: 582 DVLVHQ 599
Score = 53.8 bits (111), Expect(2) = 6e-028
Identities = 27/46 (58%), Positives = 29/46 (63%)
Frame = -1 / +1
Query: 394 AVFLYLYQLAMTR*ARKLRRLSTASSQKK*SRWPRLRFSGIHPMRP 257
A FL L LA T +K R+LS ASSQKK SR P R SGIHP P
Sbjct: 229 AAFLLLNLLAHTTYTKKFRKLSPASSQKKLSRRPSFRLSGIHPGLP 366
>gb|AV931970.1|AV931970 AV931970 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd26p14 3', mRNA sequence
Length = 680
Score = 146 bits (314), Expect = 8e-035
Identities = 57/87 (65%), Positives = 65/87 (74%)
Frame = +3 / -1
Query: 45 VPISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLSXXX 224
VP+SI WQIPQYF++G +EVFTF+G LEFFYDQ+PDAMRS+CSAL L T ALGNYLS
Sbjct: 548 VPLSILWQIPQYFLVGASEVFTFIGALEFFYDQSPDAMRSLCSALQLITTALGNYLSAFI 369
Query: 225 XXXXXXXXXRHGRIGWIPENLNRGHLD 305
R GR GWIP+NLN G LD
Sbjct: 368 LTMVAYFTTRGGRPGWIPDNLNEGRLD 288
Score = 90.0 bits (190), Expect(2) = 6e-028
Identities = 42/66 (63%), Positives = 45/66 (68%)
Frame = -3 / +3
Query: 221 DRAEVVAERDGGERQRGAHAPHGVGRLVVEELQLPHEGEHLRAPDDEVLRYLPADGDGHD 42
+RAEVV ER G E QR A APHGV RLVVEEL+ P EGEHLR D EVLR LP D +
Sbjct: 372 ERAEVVPERRGDELQRAAQAPHGVRRLVVEELERPDEGEHLRGADQEVLRDLPQDAQWNR 551
Query: 41 VVLAVQ 24
VL Q
Sbjct: 552 DVLVHQ 569
Score = 53.8 bits (111), Expect(2) = 6e-028
Identities = 27/46 (58%), Positives = 29/46 (63%)
Frame = -1 / +1
Query: 394 AVFLYLYQLAMTR*ARKLRRLSTASSQKK*SRWPRLRFSGIHPMRP 257
A FL L LA T +K R+LS ASSQKK SR P R SGIHP P
Sbjct: 199 AAFLLLNLLAHTTYTKKFRKLSPASSQKKLSRRPSFRLSGIHPGLP 336
>gb|BQ659812.1|BQ659812 HF01M18w HF Hordeum vulgare subsp. vulgare cDNA clone HF01M18
3-PRIME, mRNA sequence
Length = 682
Score = 127 bits (271), Expect = 7e-029
Identities = 49/87 (56%), Positives = 61/87 (70%)
Frame = +3 / -2
Query: 45 VPISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLSXXX 224
V +SI WQ PQY +IG EVFT +GQ EFFY+Q+PD+MRS+CSA +L TV+LG+YLS
Sbjct: 525 VSMSILWQAPQYLLIGVGEVFTSIGQAEFFYNQSPDSMRSLCSAFALVTVSLGSYLSSFI 346
Query: 225 XXXXXXXXXRHGRIGWIPENLNRGHLD 305
R +GWIP+NLN GHLD
Sbjct: 345 LTLVSYLTTRGEEMGWIPDNLNEGHLD 265
Score = 71.2 bits (149), Expect(2) = 9e-024
Identities = 31/53 (58%), Positives = 35/53 (66%)
Frame = -1 / +2
Query: 202 PSATVVSDSAEHMLLMASGAWS*KNSSCPTKVNTSAHPMMKYCGICQQMEMGT 44
PS TV AEH LLM SG W *KNS+CP VNTS P+ +Y G CQ+M M T
Sbjct: 368 PSDTVTRAKAEHRLLMESGDWL*KNSACPMDVNTSPTPISRY*GACQRMLMET 526
Score = 58.3 bits (121), Expect(2) = 9e-024
Identities = 27/47 (57%), Positives = 31/47 (65%)
Frame = -1 / +2
Query: 403 *SSAVFLYLYQLAMTR*ARKLRRLSTASSQKK*SRWPRLRFSGIHPM 263
*S+ FL+LY A R AR+ R L A SQKK SRWP R SGIHP+
Sbjct: 167 *STEAFLHLYCCAK*RKARRFRELRPAMSQKKRSRWPSFRLSGIHPI 307
>gb|AV834230.1|AV834230 AV834230 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags7k20, mRNA sequence
Length = 628
Score = 111 bits (238), Expect = 3e-024
Identities = 44/85 (51%), Positives = 56/85 (65%)
Frame = +3 / -1
Query: 51 ISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLSXXXXX 230
+SI WQ+PQYF+I AE F + QLEFF+ QAPD+M+SM +A +L T ALGNYLS
Sbjct: 478 LSIVWQLPQYFVIAGAECFVIIAQLEFFHGQAPDSMKSMLTAFALLTTALGNYLSSAIIT 299
Query: 231 XXXXXXXRHGRIGWIPENLNRGHLD 305
GWIP++LN+GHLD
Sbjct: 298 LVAGLTREWQSPGWIPDDLNQGHLD 224
>gb|AJ435085.1|AJ435085 AJ435085 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200019H08F1, mRNA sequence
Length = 525
Score = 111 bits (238), Expect = 3e-024
Identities = 44/85 (51%), Positives = 56/85 (65%)
Frame = +3 / +3
Query: 51 ISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLSXXXXX 230
+SI WQ+PQYF+I AE F + QLEFF+ QAPD+M+SM +A +L T ALGNYLS
Sbjct: 96 LSIVWQLPQYFVIAGAECFVIIAQLEFFHGQAPDSMKSMLTAFALLTTALGNYLSSAIIT 275
Query: 231 XXXXXXXRHGRIGWIPENLNRGHLD 305
GWIP++LN+GHLD
Sbjct: 276 LVAGLTREWQSPGWIPDDLNQGHLD 350
>gb|BQ134693.1|BQ134693 LP30190 Lemma/palea-enriched cDNA library from dough stage of
kernel Hordeum vulgare subsp. vulgare cDNA clone 3-190
similar to Peptide transporter, similar to 15076661,
mRNA sequence
Length = 521
Score = 105 bits (224), Expect = 2e-022
Identities = 42/69 (60%), Positives = 49/69 (71%)
Frame = +3 / +1
Query: 99 EVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLSXXXXXXXXXXXXRHGRIGWIP 278
EVFT +GQLEFFYDQAPDAMRS+C+A +L TV+ G+YLS R G GWIP
Sbjct: 1 EVFTNIGQLEFFYDQAPDAMRSLCAAFALVTVSAGSYLSSFILTLVSYVTTRGGDPGWIP 180
Query: 279 ENLNRGHLD 305
+NLN GHLD
Sbjct: 181 DNLNEGHLD 207
>gb|BJ466430.1|BJ466430 BJ466430 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags32b11 3', mRNA sequence
Length = 595
Score = 104 bits (222), Expect = 4e-022
Identities = 39/85 (45%), Positives = 54/85 (63%)
Frame = +3 / -2
Query: 51 ISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLSXXXXX 230
+SI WQ+PQ+ +I C++VF + QLEFFY +AP +MRS+CSA S ++LG YL+
Sbjct: 456 LSIAWQLPQFMLIACSDVFCGIAQLEFFYSEAPMSMRSLCSAFSFLAMSLGYYLNSMIIS 277
Query: 231 XXXXXXXRHGRIGWIPENLNRGHLD 305
G GW+P +LN GHLD
Sbjct: 276 AIAALSKSGGGKGWLPADLNDGHLD 202
>gb|BJ468216.1|BJ468216 BJ468216 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags37k17 3', mRNA sequence
Length = 699
Score = 104 bits (222), Expect = 4e-022
Identities = 39/85 (45%), Positives = 54/85 (63%)
Frame = +3 / -1
Query: 51 ISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLSXXXXX 230
+SI WQ+PQ+ +I C++VF + QLEFFY +AP +MRS+CSA S ++LG YL+
Sbjct: 474 LSIAWQLPQFMLIACSDVFCGIAQLEFFYSEAPMSMRSLCSAFSFLAMSLGYYLNSMIIS 295
Query: 231 XXXXXXXRHGRIGWIPENLNRGHLD 305
G GW+P +LN GHLD
Sbjct: 294 AIAALSKSGGGKGWLPADLNDGHLD 220
>gb|BJ458380.1|BJ458380 BJ458380 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak31m11 3', mRNA sequence
Length = 472
Score = 104 bits (221), Expect = 6e-022
Identities = 39/85 (45%), Positives = 57/85 (67%)
Frame = +3 / -3
Query: 51 ISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLSXXXXX 230
++I WQ P Y ++G AEVFT VG +EFFYD++P++M+SM +AL+ ++ GNYL+
Sbjct: 470 LNIMWQTPSYVVLGVAEVFTSVGIMEFFYDESPESMKSMGAALAQLAISAGNYLNSAVLG 291
Query: 231 XXXXXXXRHGRIGWIPENLNRGHLD 305
R G GWIP++L+ GHLD
Sbjct: 290 VVASATGRGGAPGWIPDDLDVGHLD 216
>gb|BU993945.1|BU993945 HM05A23r HM Hordeum vulgare subsp. vulgare cDNA clone HM05A23
5-PRIME, mRNA sequence
Length = 657
Score = 102 bits (217), Expect = 2e-021
Identities = 41/118 (34%), Positives = 61/118 (51%)
Frame = +3 / +1
Query: 36 DDIVPISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLS 215
D +VP+S+ W +PQY ++G A+VF VG EFFYDQ P ++S+ AL L+ + +G+++S
Sbjct: 223 DAVVPMSLWWIVPQYVLLGAADVFAMVGMQEFFYDQMPVELKSLGLALYLSVLGVGSFIS 402
Query: 216 XXXXXXXXXXXXRHGRIGWIPENLNRGHLDXXXXXXXXXXXXXXXXXXXIASWYKYKK 389
R G W +NLNRGHLD ++ Y YK+
Sbjct: 403 SFLISVIDSLTKRGGGTSWFADNLNRGHLDYFYVLLAALSALELLAFLYFSATYVYKR 576
>gb|BG301085.2|BG301085 HVSMEb0019I02f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0019I02f, mRNA sequence
Length = 541
Score = 100 bits (212), Expect = 1e-020
Identities = 37/81 (45%), Positives = 51/81 (62%)
Frame = +3 / +1
Query: 63 WQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLSXXXXXXXXX 242
WQ+PQ+ +I C++VF + QLEFFY +AP +MRS+CSA S ++LG YL+
Sbjct: 1 WQLPQFMLIACSDVFCGIAQLEFFYSEAPMSMRSLCSAFSFLAMSLGYYLNSMIISAIAA 180
Query: 243 XXXRHGRIGWIPENLNRGHLD 305
G GW+P +LN GHLD
Sbjct: 181 LSKSGGGKGWLPADLNDGHLD 243
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 260,660,745
Number of Sequences: 312970
Number of extensions: 3739696
Number of successful extensions: 75290
Number of sequences better than 1.0e-020: 20
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6362506350
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)