TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3203235.2.1
(637 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM373233.2|BM373233 EBma04_SQ003_K04_R maternal, 10 DPA,... 151 3e-036
gb|BF623053.2|BF623053 HVSMEa0012M22f Hordeum vulgare seedl... 147 6e-035
gb|BQ767981.1|BQ767981 EBro08_SQ010_J07_R root, 3 week, dro... 146 9e-035
gb|BM371229.2|BM371229 EBro04_SQ003_O11_R root, 3 week, sal... 145 2e-034
gb|AL507983.1|AL507983 AL507983 Hordeum vulgare Barke devel... 139 1e-032
gb|BF628008.2|BF628008 HVSMEb0002J11f Hordeum vulgare seedl... 139 1e-032
gb|CA014589.1|CA014589 HT11M09r HT Hordeum vulgare subsp. v... 134 5e-031
gb|BF259970.1|BF259970 HVSMEf0020M03f Hordeum vulgare seedl... 126 1e-028
gb|BI953372.1|BI953372 HVSMEm0012F09f Hordeum vulgare green... 124 4e-028
gb|BF621150.3|BF621150 HVSMEa0004J06f Hordeum vulgare seedl... 123 7e-028
gb|BM097354.2|BM097354 EBro01_SQ004_O08_R root, 3 week, hyd... 122 1e-027
gb|BF260721.2|BF260721 HVSMEf0022M07f Hordeum vulgare seedl... 117 6e-026
gb|BF258425.1|BF258425 HVSMEf0015L01f Hordeum vulgare seedl... 109 2e-023
gb|BQ763476.1|BQ763476 EBro02_SQ008_B17_R root, 3 week, hyd... 106 1e-022
gb|BU980354.1|BU980354 HA20H10r HA Hordeum vulgare subsp. v... 100 8e-021
>gb|BM373233.2|BM373233 EBma04_SQ003_K04_R maternal, 10 DPA, no treatment, cv Optic, EBma04
Hordeum vulgare subsp. vulgare cDNA clone
EBma04_SQ003_K04 5', mRNA sequence
Length = 577
Score = 151 bits (325), Expect = 3e-036
Identities = 61/153 (39%), Positives = 101/153 (66%)
Frame = +2 / +1
Query: 140 PHVQMVLAQLCYTLMYFITEAAFNQGLNPYVYITYRHLLVAVLIWPFAYYLEKGLRPKMT 319
P++ M+L Q+ Y MY ++ A+ +G++ YV +TYR+L+ +++ PFA EKG+RPKMT
Sbjct: 16 PYMMMILLQIGYAGMYIVSVASLKRGMSHYVLVTYRNLVATLVMLPFALVFEKGVRPKMT 195
Query: 320 LMLFVEIFVLSLLGVSLTLNMYFTSLKYTSPTFVTSVVNTIASMTFVIAIILRMEIVDVK 499
L +F++I L+LL L N+Y+ K TS +F +++VN + ++TF++AI+LRME + ++
Sbjct: 196 LRIFIKILGLALLEPVLDQNLYYVGNKLTSASFSSALVNILPAVTFIMAIVLRMEKLRLR 375
Query: 500 SLRGVAKVAGTVVSFAGVTTMTLYKGAAITSLW 598
S AKVAGT+ + G M +Y G + W
Sbjct: 376 SSHSQAKVAGTICTVIGAVLMIMYHGPVVQFPW 474
>gb|BF623053.2|BF623053 HVSMEa0012M22f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0012M22f, mRNA sequence
Length = 833
Score = 147 bits (315), Expect = 6e-035
Identities = 60/136 (44%), Positives = 90/136 (66%)
Frame = +2 / +2
Query: 185 YFITEAAFNQGLNPYVYITYRHLLVAVLIWPFAYYLEKGLRPKMTLMLFVEIFVLSLLGV 364
+ ++ AA N G++ V+I YR+++ L+ PFAY+LEK RP +TL L E FVL+L+G+
Sbjct: 176 HIVSRAALNMGISKLVFIVYRNVISLALLAPFAYFLEKKDRPPLTLSLLAEFFVLALIGI 355
Query: 365 SLTLNMYFTSLKYTSPTFVTSVVNTIASMTFVIAIILRMEIVDVKSLRGVAKVAGTVVSF 544
+ Y L + SPT+ +++ N + ++TFV+A +LR+E VD+ GVAKV GTVVS
Sbjct: 356 TANQGFYLLGLYHLSPTYASAIQNMVPAITFVLAAVLRLEQVDLGRRHGVAKVVGTVVSV 535
Query: 545 AGVTTMTLYKGAAITS 592
G T +TLYKG + S
Sbjct: 536 GGATVITLYKGLPLFS 583
>gb|BQ767981.1|BQ767981 EBro08_SQ010_J07_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ010_J07 5', mRNA sequence
Length = 611
Score = 146 bits (314), Expect = 9e-035
Identities = 61/143 (42%), Positives = 95/143 (66%)
Frame = +2 / +3
Query: 140 PHVQMVLAQLCYTLMYFITEAAFNQGLNPYVYITYRHLLVAVLIWPFAYYLEKGLRPKMT 319
P++ MVL Q+ + MY I A+ G++ +V + YR+L+ ++ PFA Y E+GLRPKMT
Sbjct: 183 PYLAMVLLQVGFAGMYIIAVASLKAGMSHFVLVVYRNLVATAVMMPFAIYFERGLRPKMT 362
Query: 320 LMLFVEIFVLSLLGVSLTLNMYFTSLKYTSPTFVTSVVNTIASMTFVIAIILRMEIVDVK 499
+ +F++I L+ L + N+YF K TS F T++VN + ++TFV+A+ILRME V ++
Sbjct: 363 ITIFIKIMGLAFLEPVIDQNLYFMGAKMTSAGFATALVNILPAVTFVLALILRMEKVRLR 542
Query: 500 SLRGVAKVAGTVVSFAGVTTMTL 568
SL AK+AGT+++ AG M L
Sbjct: 543 SLHSQAKIAGTLLTVAGAVLMVL 611
>gb|BM371229.2|BM371229 EBro04_SQ003_O11_R root, 3 week, salt-stressed, cv Optic, EBro04
Hordeum vulgare subsp. vulgare cDNA clone
EBro04_SQ003_O11 5', mRNA sequence
Length = 597
Score = 145 bits (311), Expect = 2e-034
Identities = 61/143 (42%), Positives = 95/143 (66%)
Frame = +2 / +1
Query: 140 PHVQMVLAQLCYTLMYFITEAAFNQGLNPYVYITYRHLLVAVLIWPFAYYLEKGLRPKMT 319
P++ MVL Q+ + MY I A+ +G++ +V + YR+L+ ++ PFA Y E+GLRPKMT
Sbjct: 169 PYLAMVLLQVGFAGMYIIAVASLKRGMSHFVLVVYRNLVATAVMAPFALYFERGLRPKMT 348
Query: 320 LMLFVEIFVLSLLGVSLTLNMYFTSLKYTSPTFVTSVVNTIASMTFVIAIILRMEIVDVK 499
+ +F++I L+ L L N+Y+ TS F T++VN + ++TFV+A+ILRME V ++
Sbjct: 349 ITIFIKIMGLAFLEPVLDQNLYYMGANLTSAGFATALVNILPAVTFVLALILRMEKVRLR 528
Query: 500 SLRGVAKVAGTVVSFAGVTTMTL 568
SL AK+AGTV++ AG M L
Sbjct: 529 SLHSQAKIAGTVLTVAGAVLMVL 597
>gb|AL507983.1|AL507983 AL507983 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY07G20V 5',
mRNA sequence
Length = 700
Score = 139 bits (299), Expect = 1e-032
Identities = 63/163 (38%), Positives = 100/163 (61%)
Frame = +2 / +3
Query: 140 PHVQMVLAQLCYTLMYFITEAAFNQGLNPYVYITYRHLLVAVLIWPFAYYLEKGLRPKMT 319
P+V V+ Q+ YT M+ +++AAFNQG N YV+I YR ++L+ P A E+ M+
Sbjct: 93 PYVIAVIIQVIYTGMFVVSKAAFNQGFNTYVFIFYRQAAASLLLLPLAIAFERKNARAMS 272
Query: 320 LMLFVEIFVLSLLGVSLTLNMYFTSLKYTSPTFVTSVVNTIASMTFVIAIILRMEIVDVK 499
L +++F+ +L+G + +LN+Y SLK TS T ++ N++ +TF IA++LRME+V ++
Sbjct: 273 FRLLLKLFLFALIGTTFSLNLYNVSLKLTSATVGSATSNSMPVVTFFIALLLRMEVVKLR 452
Query: 500 SLRGVAKVAGTVVSFAGVTTMTLYKGAAITSLWKSPVQIAGSG 628
S G+AKVAG + AGV + Y G ++ L V A G
Sbjct: 453 SPSGMAKVAGVGLCLAGVLVIAFYVGPSLNPLNHHRVLAAAIG 581
>gb|BF628008.2|BF628008 HVSMEb0002J11f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0002J11f, mRNA sequence
Length = 665
Score = 139 bits (298), Expect = 1e-032
Identities = 59/136 (43%), Positives = 87/136 (63%)
Frame = +2 / +3
Query: 185 YFITEAAFNQGLNPYVYITYRHLLVAVLIWPFAYYLEKGLRPKMTLMLFVEIFVLSLLGV 364
+ ++ AA N G++ V+I YR+++ L+ PFAY+LEK RP +TL L E FVL+L+G+
Sbjct: 96 HIVSRAALNMGISKLVFIVYRNVISLALLAPFAYFLEKKDRPPLTLSLLAEFFVLALIGI 275
Query: 365 SLTLNMYFTSLKYTSPTFVTSVVNTIASMTFVIAIILRMEIVDVKSLRGVAKVAGTVVSF 544
+ Y L + SPT+ +++ N ++TFV+A LR+E VD+ GVA V GTVVS
Sbjct: 276 TANQGFYLLGLYHLSPTYASAIQNMEPAITFVLAAELRLEQVDLGRRHGVANVVGTVVSV 455
Query: 545 AGVTTMTLYKGAAITS 592
G T +TLYKG + S
Sbjct: 456 GGATVITLYKGLPLFS 503
>gb|CA014589.1|CA014589 HT11M09r HT Hordeum vulgare subsp. vulgare cDNA clone HT11M09
5-PRIME, mRNA sequence
Length = 671
Score = 134 bits (287), Expect = 5e-031
Identities = 60/146 (41%), Positives = 89/146 (60%)
Frame = +2 / +1
Query: 140 PHVQMVLAQLCYTLMYFITEAAFNQGLNPYVYITYRHLLVAVLIWPFAYYLEKGLRPKMT 319
P V MVL QL + M +++ A + G++PYV I YR+L+ + + P AYY E+ +T
Sbjct: 148 PTVAMVLVQLGFAGMNVLSKLALDTGMSPYVLIAYRNLIAGMFLAPGAYYFERRSGMVIT 327
Query: 320 LMLFVEIFVLSLLGVSLTLNMYFTSLKYTSPTFVTSVVNTIASMTFVIAIILRMEIVDVK 499
+ ++IF S+ G +L +YF LK T+PT ++ NT+ ++TFV+A IL+ME V +K
Sbjct: 328 KQVLIQIFFSSIFGATLNQVLYFVGLKSTTPTVACALSNTLPALTFVMAAILKMETVRLK 507
Query: 500 SLRGVAKVAGTVVSFAGVTTMTLYKG 577
+ G AKV GTVV G M YKG
Sbjct: 508 TSSGKAKVLGTVVCVGGSMVMPFYKG 585
>gb|BF259970.1|BF259970 HVSMEf0020M03f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0020M03f, mRNA sequence
Length = 549
Score = 126 bits (269), Expect = 1e-028
Identities = 51/99 (51%), Positives = 73/99 (73%)
Frame = +2 / +2
Query: 302 LRPKMTLMLFVEIFVLSLLGVSLTLNMYFTSLKYTSPTFVTSVVNTIASMTFVIAIILRM 481
+RP+MT ++F+ IF+L+L+G + N Y+ LKYTSPTF ++ N + +MTFV+A+I RM
Sbjct: 26 VRPRMTPLVFLNIFLLALMGPVIDQNFYYAGLKYTSPTFSCAMSNMLPAMTFVMAVIFRM 205
Query: 482 EIVDVKSLRGVAKVAGTVVSFAGVTTMTLYKGAAITSLW 598
E VD+K R +AKVAGT+V+ AG MTLYKG A+ +W
Sbjct: 206 EKVDLKKARCLAKVAGTLVTVAGAMLMTLYKGRAVQMVW 322
>gb|BI953372.1|BI953372 HVSMEm0012F09f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0012F09f, mRNA sequence
Length = 831
Score = 124 bits (266), Expect = 4e-028
Identities = 52/116 (44%), Positives = 77/116 (66%)
Frame = +2 / +1
Query: 245 RHLLVAVLIWPFAYYLEKGLRPKMTLMLFVEIFVLSLLGVSLTLNMYFTSLKYTSPTFVT 424
R+++ L+ PFAY+LEK RP +TL L E FVL+L+G++ Y + + SPT+ +
Sbjct: 4 RNVISLALLAPFAYFLEKKDRPPLTLSLLAEFFVLALIGITANQGFYLLGVCHLSPTYAS 183
Query: 425 SVVNTIASMTFVIAIILRMEIVDVKSLRGVAKVAGTVVSFAGVTTMTLYKGAAITS 592
++ N + ++TFV+A +LR+E VD+ GVAKV GTVVS G T +TLYKG + S
Sbjct: 184 AIQNMVPAITFVLAAVLRLEQVDLGRRHGVAKVVGTVVSVCGATVITLYKGLPLFS 351
>gb|BF621150.3|BF621150 HVSMEa0004J06f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0004J06f, mRNA sequence
Length = 735
Score = 123 bits (264), Expect = 7e-028
Identities = 52/109 (47%), Positives = 73/109 (66%)
Frame = +2 / +3
Query: 266 LIWPFAYYLEKGLRPKMTLMLFVEIFVLSLLGVSLTLNMYFTSLKYTSPTFVTSVVNTIA 445
L+ PFAY+LEK RP +TL L E FVL+L+G++ Y L + SPT+ +++ N +
Sbjct: 3 LLAPFAYFLEKKDRPPLTLSLLAEFFVLALIGITANQGFYLLGLYHLSPTYASAIQNMVP 182
Query: 446 SMTFVIAIILRMEIVDVKSLRGVAKVAGTVVSFAGVTTMTLYKGAAITS 592
++TFV+A +LR+E VD+ GVAKV GTVVS G T +TLYKG + S
Sbjct: 183 AITFVLAAVLRLEQVDLGRRHGVAKVVGTVVSVGGATVITLYKGLPLFS 329
>gb|BM097354.2|BM097354 EBro01_SQ004_O08_R root, 3 week, hydroponic grown, no treatment, cv
Optic, EBro01 Hordeum vulgare subsp. vulgare cDNA clone
EBro01_SQ004_O08 5', mRNA sequence
Length = 534
Score = 122 bits (262), Expect = 1e-027
Identities = 48/114 (42%), Positives = 81/114 (71%)
Frame = +2 / +2
Query: 140 PHVQMVLAQLCYTLMYFITEAAFNQGLNPYVYITYRHLLVAVLIWPFAYYLEKGLRPKMT 319
P++ M+L Q+ Y MY ++ A+ +G++ YV +TYR+L+ +++ PFA EKG+RPKMT
Sbjct: 191 PYMMMILLQIGYAGMYIVSVASLKRGMSHYVLVTYRNLVATLVMLPFALVFEKGVRPKMT 370
Query: 320 LMLFVEIFVLSLLGVSLTLNMYFTSLKYTSPTFVTSVVNTIASMTFVIAIILRM 481
L +F++I L+LL L N+Y+ K TS +F +++VN + ++TF++AI+LRM
Sbjct: 371 LRIFIKILGLALLEPVLDQNLYYVGNKLTSASFSSALVNILPAVTFIMAIVLRM 532
>gb|BF260721.2|BF260721 HVSMEf0022M07f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0022M07f, mRNA sequence
Length = 542
Score = 117 bits (250), Expect = 6e-026
Identities = 50/122 (40%), Positives = 79/122 (64%)
Frame = +2 / +2
Query: 140 PHVQMVLAQLCYTLMYFITEAAFNQGLNPYVYITYRHLLVAVLIWPFAYYLEKGLRPKMT 319
P++ MVL Q+ + MY I A+ G++ +V + YR+L+ ++ PF Y +GLRPKMT
Sbjct: 176 PYLAMVLLQVGFAGMYIIAVASLMAGMSHFVLVVYRNLVGTAVMMPFVIYFVRGLRPKMT 355
Query: 320 LMLFVEIFVLSLLGVSLTLNMYFTSLKYTSPTFVTSVVNTIASMTFVIAIILRMEIVDVK 499
+ +F++I L+ L + N+YF K TS F T++VN + ++TFV+A+ILRME V +
Sbjct: 356 ITMFIKIMGLAFLEPVIDQNLYFMGAKMTSAGFATALVNILPAVTFVLALILRMENVRMP 535
Query: 500 SL 505
SL
Sbjct: 536 SL 541
>gb|BF258425.1|BF258425 HVSMEf0015L01f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0015L01f, mRNA sequence
Length = 620
Score = 109 bits (232), Expect = 2e-023
Identities = 46/99 (46%), Positives = 66/99 (66%)
Frame = +2 / +1
Query: 302 LRPKMTLMLFVEIFVLSLLGVSLTLNMYFTSLKYTSPTFVTSVVNTIASMTFVIAIILRM 481
+RP+MT ++F+ IF+L L+G + N Y+ L YT PTF ++ N + +MTFV+A I RM
Sbjct: 28 VRPRMTHLVFLNIFLLDLMGPVIHQNFYYAGLMYTYPTFSCAMSNMLPAMTFVMADIFRM 207
Query: 482 EIVDVKSLRGVAKVAGTVVSFAGVTTMTLYKGAAITSLW 598
E VD+ +A VAGT+V+ AG MTLYKG A+ +W
Sbjct: 208 EKVDLTKAMCLANVAGTLVTVAGAMLMTLYKGRAVQMVW 324
>gb|BQ763476.1|BQ763476 EBro02_SQ008_B17_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ008_B17 5', mRNA sequence
Length = 536
Score = 106 bits (226), Expect = 1e-022
Identities = 46/125 (36%), Positives = 74/125 (59%)
Frame = +2 / +3
Query: 143 HVQMVLAQLCYTLMYFITEAAFNQGLNPYVYITYRHLLVAVLIWPFAYYLEKGLRPKMTL 322
HV M+ Q Y + ++ A N G++ V+ YR+++ L+ PFAY+LEK RP++TL
Sbjct: 120 HVAMLALQFGYAGFHVVSRLALNMGISKLVFPVYRNIIALFLLVPFAYFLEKKDRPRLTL 299
Query: 323 MLFVEIFVLSLLGVSLTLNMYFTSLKYTSPTFVTSVVNTIASMTFVIAIILRMEIVDVKS 502
V+ F L+L G++ Y L TSPTF +++ N++ ++TF +A LR+E V +
Sbjct: 300 GFAVQFFFLALCGITANQGFYLLGLDNTSPTFASAIQNSVPAITFGMAAALRIEKVRLDR 479
Query: 503 LRGVA 517
GVA
Sbjct: 480 RDGVA 494
>gb|BU980354.1|BU980354 HA20H10r HA Hordeum vulgare subsp. vulgare cDNA clone HA20H10
5-PRIME, mRNA sequence
Length = 502
Score = 100 bits (213), Expect = 8e-021
Identities = 47/132 (35%), Positives = 77/132 (58%)
Frame = +2 / +3
Query: 233 YITYRHLLVAVLIWPFAYYLEKGLRPKMTLMLFVEIFVLSLLGVSLTLNMYFTSLKYTSP 412
+I YR ++L+ P A E+ M+ L +++F+ +L+G + +LN+Y SLK TS
Sbjct: 12 FIFYRQAAASLLLLPLAIAFERKNARAMSFRLLLKLFLFALIGTTFSLNLYNVSLKLTSA 191
Query: 413 TFVTSVVNTIASMTFVIAIILRMEIVDVKSLRGVAKVAGTVVSFAGVTTMTLYKGAAITS 592
T ++ N++ +TF IA++LRME+V ++S G+AKVAG + AGV + Y G ++
Sbjct: 192 TVGSATSNSMPVVTFFIALLLRMEVVKLRSPSGMAKVAGVGLCLAGVLVIAFYVGPSLNP 371
Query: 593 LWKSPVQIAGSG 628
L V A G
Sbjct: 372 LNHHRVLAAAIG 407
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 213,662,251
Number of Sequences: 312970
Number of extensions: 2695952
Number of successful extensions: 43448
Number of sequences better than 1.0e-020: 17
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6786673440
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)