TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3198766.2.1
(1273 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI960587.1|BI960587 HVSMEn0025E05f Hordeum vulgare rachi... 275 5e-073
gb|BI959076.1|BI959076 HVSMEn0018A17f Hordeum vulgare rachi... 115 9e-061
gb|BM442096.2|BM442096 EBan01_SQ002_C23_R anther, yellow st... 233 1e-060
gb|BU981349.1|BU981349 HA23F03r HA Hordeum vulgare subsp. v... 226 2e-058
gb|BU995294.1|BU995294 HM09M17r HM Hordeum vulgare subsp. v... 179 1e-049
gb|AW983378.3|AW983378 HVSMEg0010G22f Hordeum vulgare pre-a... 181 7e-045
gb|AV915392.1|AV915392 AV915392 K. Sato unpublished cDNA li... 167 9e-041
gb|CA001634.1|CA001634 HS05C08r HS Hordeum vulgare subsp. v... 162 4e-039
gb|CB881433.1|CB881433 HM09M17w HM Hordeum vulgare subsp. v... 133 1e-030
gb|BQ764302.1|BQ764302 EBan01_SQ005_P07_R anther, yellow st... 105 4e-022
>gb|BI960587.1|BI960587 HVSMEn0025E05f Hordeum vulgare rachis EST library HVcDNA0015 (normal)
Hordeum vulgare subsp. vulgare cDNA clone HVSMEn0025E05f,
mRNA sequence
Length = 663
Score = 275 bits (594), Expect = 5e-073
Identities = 106/200 (53%), Positives = 142/200 (71%)
Frame = +2 / +1
Query: 500 FGSTNVWLDHLSLSNCQDGLIDVIAKSTGVTISNCHLTNHNDVMLFSSSDKHPEDQIMQI 679
FG+++VW+DH SLSNC DGLID I ST +T+SN + T+HN+VML SD + +D+ MQ+
Sbjct: 28 FGASHVWVDHCSLSNCADGLIDAIMGSTAITVSNNYFTHHNEVMLLGHSDSYLKDKAMQV 207
Query: 680 TVAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSKAPTIISQGNRYIAPPNLA 859
T+AFNHFG GL+QRMPRCR G+FHVVNNDYTHW MYAIGGS PTI SQGNRY+AP N
Sbjct: 208 TIAFNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNRYLAPTNPF 387
Query: 860 AKQVTKQHDTPESVWKNWVWHSENDLFMEGAYFTVTGGQINRQFNKKDLIKPRNGSYVTR 1039
AK+VTK+ +T ++ WK W W SE D+ + GA+FT + + +++ + ++ S V
Sbjct: 388 AKEVTKRVETAQTTWKAWNWRSEGDMLLNGAFFTPSRAXASAIYSRAXSLGGKSPSMVAT 567
Query: 1040 LTRYAGSLACTPGKPC*IKP 1099
+T AG+L+C P P
Sbjct: 568 ITSGAGALSCHKAPPAITAP 627
Score = 167 bits (360), Expect = 9e-041
Identities = 84/154 (54%), Positives = 97/154 (62%)
Frame = -2 / -2
Query: 960 VK*APSMKRSFSECHTQFFHTDSGVSCCLVTCFAARLGGAM*RLPWLMMVGALLPPMAYM 781
VK AP S S+ Q FH VS VT A L GA *R PWL+MVG+ LPPMAY+
Sbjct: 488 VKKAPFSSMSPSDLQFQAFHVVWAVSTLFVTSLAKGLVGAR*RFPWLLMVGSALPPMAYI 309
Query: 780 SQCV*SLLTTWKKPQRHLGILCTRPLPKWLKATVICMIWSSGCLSLELKSMTSLWLVRWQ 601
SQ V*SL TTWK P HLGIL P PKWLKA V C+ S SL +S+TSLW V+
Sbjct: 308 SQ*V*SLFTTWK*PCLHLGILWMSPSPKWLKAMVTCIALSLR*ESL*PRSITSLWWVK*L 129
Query: 600 LEMVTPVDLAMTSMRPSWQLERERWSSHTLVEPK 499
L+ V V+ M SMRPS QLERE+WS+ T + PK
Sbjct: 128 LDTVMAVEPMMASMRPSAQLEREQWSTQTWLAPK 27
>gb|BI959076.1|BI959076 HVSMEn0018A17f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0018A17f, mRNA sequence
Length = 632
Score = 115 bits (246), Expect(3) = 9e-061
Identities = 40/73 (54%), Positives = 55/73 (75%)
Frame = +2 / +3
Query: 437 VRDSPDHFGWRTVSDGDGITVFGSTNVWLDHLSLSNCQDGLIDVIAKSTGVTISNCHLTN 616
VR SP H+GWRT++DGD + +FG+++VW+DH SLSNC DGLID I ST +T+SN + T+
Sbjct: 411 VRSSPSHYGWRTIADGDAVXIFGASHVWVDHCSLSNCADGLIDAIMGSTAITVSNNYFTH 590
Query: 617 HNDVMLFSSSDKH 655
HN+ ML D +
Sbjct: 591 HNEGMLLGHRDSY 629
Score = 86.8 bits (183), Expect(3) = 9e-061
Identities = 35/60 (58%), Positives = 44/60 (73%)
Frame = +2 / +3
Query: 188 NPRPGTLRWGVIQIEPLWITFAKTMIITLKEELIIRGDKTIDGRGVQVRITNGAQLTLQF 367
+P+ GTLR+ VIQ EPLWI F + M+ITL +ELI+ KTIDGRG V I NGA T+Q+
Sbjct: 168 DPKKGTLRYAVIQDEPLWIIFKRDMVITLSQELIMNSFKTIDGRGANVHIANGACTTIQY 347
Score = 75.8 bits (159), Expect(3) = 4e-034
Identities = 36/71 (50%), Positives = 44/71 (61%)
Frame = -2 / -2
Query: 648 SLELKSMTSLWLVRWQLEMVTPVDLAMTSMRPSWQLERERWSSHTLVEPKTVMPSPSETV 469
SL +S+ SLW V+ L+ V V+ M SM PS QLERE+WS+ T + PK SPS V
Sbjct: 622 SL*PRSIPSLWWVK*LLDTVMAVEPMMASMSPSAQLEREQWSTQTWLAPKMXTASPSAMV 443
Query: 468 RQPKWSGESRT 436
R P W GE RT
Sbjct: 442 RHP*WLGELRT 410
Score = 71.2 bits (149), Expect(3) = 9e-061
Identities = 27/56 (48%), Positives = 30/56 (53%)
Frame = +2 / +2
Query: 35 NPIDRCWRCRQDWATDRKRLARCAKGFGRNTTGGLAGKFYXXXXXXXXXXXNPRPG 202
NPID CWRC DW +R+RLA C GFGRN GG GK Y P+ G
Sbjct: 14 NPIDDCWRCDSDWHNNRQRLADCGIGFGRNAIGGRDGKIYVVTDAGDDDPGRPQEG 181
Score = 61.6 bits (128), Expect(3) = 4e-034
Identities = 28/43 (65%), Positives = 31/43 (72%)
Frame = -1 / -1
Query: 346 AVGDAHLHAAAVNGLVAADDELLLKRDDHGLGEGDPQRLDLDD 218
AVGD H+ AAAV+GL A DELL + DDH E DPQRL LDD
Sbjct: 326 AVGDVHVGAAAVDGLEAVHDELLAEGDDHVALEDDPQRLVLDD 198
Score = 46.4 bits (95), Expect(3) = 4e-034
Identities = 19/38 (50%), Positives = 24/38 (63%)
Frame = -2 / -3
Query: 147 LPASPPVVLRPNPLAHRARRFRSVAQSCRHRQHRSIGF 34
LP+ PP+ LRP P+ ARR R + QS RQ S+GF
Sbjct: 126 LPSRPPMALRPKPMPQSARRCRLLCQSESQRQQSSMGF 13
>gb|BM442096.2|BM442096 EBan01_SQ002_C23_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ002_C23 5', mRNA sequence
Length = 356
Score = 233 bits (504), Expect = 1e-060
Identities = 89/118 (75%), Positives = 101/118 (85%)
Frame = +2 / +2
Query: 653 HPEDQIMQITVAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSKAPTIISQGN 832
+P+DQIMQ+TVAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSK PTIISQGN
Sbjct: 2 NPKDQIMQVTVAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSKNPTIISQGN 181
Query: 833 RYIAPPNLAAKQVTKQHDTPESVWKNWVWHSENDLFMEGAYFTVTGGQINRQFNKKDL 1006
RYIAPPNLAAK +TK+ E+ WKNWVWHS++DL + A F +GG R+F+K DL
Sbjct: 182 RYIAPPNLAAKLITKRLGASEAEWKNWVWHSQDDLLLNDAVFADSGGANERKFDKDDL 355
Score = 193 bits (416), Expect = 2e-048
Identities = 83/117 (70%), Positives = 87/117 (74%)
Frame = -2 / -3
Query: 1005 RSFLLNCLLIWPPVTVK*APSMKRSFSECHTQFFHTDSGVSCCLVTCFAARLGGAM*RLP 826
RS L N L + PP++ K A RS ECHTQFFH+ S LV FAAR GGAM*R P
Sbjct: 354 RSSLSNFLSLAPPLSAKTASFSSRSSCECHTQFFHSASDAPSRLVISFAARFGGAM*RFP 175
Query: 825 WLMMVGALLPPMAYMSQCV*SLLTTWKKPQRHLGILCTRPLPKWLKATVICMIWSSG 655
WLMMVG LLPPMAYMSQ V*SL TTWK P RHLGILCTRPLPKWLKATV CMIWS G
Sbjct: 174 WLMMVGFLLPPMAYMSQWV*SLFTTWKNPHRHLGILCTRPLPKWLKATVTCMIWSLG 4
>gb|BU981349.1|BU981349 HA23F03r HA Hordeum vulgare subsp. vulgare cDNA clone HA23F03
5-PRIME, mRNA sequence
Length = 509
Score = 226 bits (488), Expect = 2e-058
Identities = 86/138 (62%), Positives = 109/138 (78%)
Frame = +2 / +3
Query: 677 ITVAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSKAPTIISQGNRYIAPPNL 856
+TVAFNHFG+GLVQRMPRCR+GFFHVVNNDYTHW MYAIGG++ PTIISQGNR+IAP +
Sbjct: 12 VTVAFNHFGKGLVQRMPRCRFGFFHVVNNDYTHWKMYAIGGNRDPTIISQGNRFIAPDDP 191
Query: 857 AAKQVTKQHDTPESVWKNWVWHSENDLFMEGAYFTVTGGQINRQFNKKDLIKPRNGSYVT 1036
AK+VTK+ TP + +K+WVW S+ D+ M GA+F +GGQ R + D I ++G YV
Sbjct: 192 NAKEVTKREYTPYNEYKDWVWKSQGDVMMNGAFFNQSGGQNERTYGNMDFIPAKHGKYVG 371
Query: 1037 RLTRYAGSLACTPGKPC* 1090
+LT++AG+L C GKPC*
Sbjct: 372 QLTQFAGTLECRVGKPC* 425
Score = 150 bits (322), Expect = 2e-035
Identities = 77/152 (50%), Positives = 90/152 (59%)
Frame = -2 / -2
Query: 1134 QVCLWSSAVLAGGLI*QGLPGVQAREPA*RVSLVT*DPFLGLMRSFLLNCLLIWPPVTVK 955
Q+ W + A * GLP + PA VS T P L M+S L WPP +K
Sbjct: 469 QLINWQARRDAIEFH*HGLPTRHSSVPANCVSCPTYLPCLAGMKSMLPYVRSFWPPDWLK 290
Query: 954 *APSMKRSFSECHTQFFHTDSGVSCCLVTCFAARLGGAM*RLPWLMMVGALLPPMAYMSQ 775
AP + S + HTQ ++ GV LVT A GAM R PWLMMVG+ LPPMAY+ Q
Sbjct: 289 KAPFIMTSPCDFHTQSLYSL*GVYSRLVTSLALGSSGAMNRFPWLMMVGSRLPPMAYIFQ 110
Query: 774 CV*SLLTTWKKPQRHLGILCTRPLPKWLKATV 679
CV*SL TTWKKP+R G+ CTRPLPKWLKATV
Sbjct: 109 CV*SLFTTWKKPKRQRGMRCTRPLPKWLKATV 14
>gb|BU995294.1|BU995294 HM09M17r HM Hordeum vulgare subsp. vulgare cDNA clone HM09M17
5-PRIME, mRNA sequence
Length = 585
Score = 179 bits (386), Expect = 2e-044
Identities = 67/102 (65%), Positives = 87/102 (85%)
Frame = +2 / +1
Query: 638 SSSDKHPEDQIMQITVAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSKAPTI 817
+S+ + +D++MQ+TVAFNHFG+GLVQRMPRCR+GFFHVVNNDYTHW MYAIGG++ PTI
Sbjct: 277 ASNAQEQQDRMMQVTVAFNHFGKGLVQRMPRCRFGFFHVVNNDYTHWKMYAIGGNRDPTI 456
Query: 818 ISQGNRYIAPPNLAAKQVTKQHDTPESVWKNWVWHSENDLFM 943
ISQGNR+IAP + AK+VTK+ TP + +K+WVW S+ D+ M
Sbjct: 457 ISQGNRFIAPDDPNAKEVTKREYTPYNEYKDWVWKSQGDVMM 582
Score = 131 bits (280), Expect(2) = 1e-049
Identities = 56/86 (65%), Positives = 63/86 (73%)
Frame = -2 / -2
Query: 918 HTQFFHTDSGVSCCLVTCFAARLGGAM*RLPWLMMVGALLPPMAYMSQCV*SLLTTWKKP 739
HTQ ++ GV LVT A GAM R PWLMMVG+ LPPMAY+ QCV*SL TTWKKP
Sbjct: 557 HTQSLYSL*GVYSRLVTSLALGSSGAMNRFPWLMMVGSRLPPMAYIFQCV*SLFTTWKKP 378
Query: 738 QRHLGILCTRPLPKWLKATVICMIWS 661
+R G+ CTRPLPKWLKATV C+I S
Sbjct: 377 KRQRGMRCTRPLPKWLKATVTCIILS 300
Score = 121 bits (259), Expect(2) = 1e-028
Identities = 44/82 (53%), Positives = 61/82 (74%)
Frame = +2 / +1
Query: 428 GGYVRDSPDHFGWRTVSDGDGITVFGSTNVWLDHLSLSNCQDGLIDVIAKSTGVTISNCH 607
GG +RDS H+G RT SDGDG+++ S+NVW+DH+S+S C DGLIDV++ ST +T+SN H
Sbjct: 64 GGMIRDSKHHYGRRTRSDGDGVSILSSSNVWIDHVSMSTCADGLIDVVSGSTAITVSNSH 243
Query: 608 LTNHNDVMLFSSSDKHPEDQIM 673
TNH+ VMLF +S+ + M
Sbjct: 244 FTNHDHVMLFGASNAQEQQDRM 309
Score = 86.8 bits (183), Expect(2) = 1e-049
Identities = 38/70 (54%), Positives = 46/70 (65%)
Frame = -2 / -2
Query: 636 KSMTSLWLVRWQLEMVTPVDLAMTSMRPSWQLERERWSSHTLVEPKTVMPSPSETVRQPK 457
KSMT WLV+W LE V V+ TSMRPS Q++ + WS HTL+E + PSPS VR+P
Sbjct: 272 KSMTWSWLVKWLLETVMAVEPLTTSMRPSAQVDMDTWSIHTLLEERMETPSPSLRVRRP* 93
Query: 456 WSGESRT*PP 427
W ESR PP
Sbjct: 92 WCLESRIMPP 63
Score = 28.1 bits (55), Expect(2) = 1e-028
Identities = 14/28 (50%), Positives = 17/28 (60%)
Frame = +1 / +3
Query: 835 LHRAAQPCREAGHQAA*HAGVRVEELGV 918
+HRA P + G QA HA RV+ LGV
Sbjct: 474 VHRAR*PQCQGGDQAGVHALQRVQGLGV 557
>gb|AW983378.3|AW983378 HVSMEg0010G22f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0010G22f, mRNA sequence
Length = 809
Score = 181 bits (390), Expect = 7e-045
Identities = 71/114 (62%), Positives = 82/114 (71%)
Frame = +2 / +2
Query: 23 CRATNPIDRCWRCRQDWATDRKRLARCAKGFGRNTTGGLAGKFYXXXXXXXXXXXNPRPG 202
C ATNPIDRCWRCR+DWATDR+RLARCAKGFGR TGGL GK Y NPRPG
Sbjct: 272 CLATNPIDRCWRCRKDWATDRQRLARCAKGFGRGATGGLHGKIYIVTDPSDEDFTNPRPG 451
Query: 203 TLRWGVIQIEPLWITFAKTMIITLKEELIIRGDKTIDGRGVQVRITNGAQLTLQ 364
TLRWGV+Q+EPLWI FA+ MII +E+II+ +K + GR QV I LT+Q
Sbjct: 452 TLRWGVVQLEPLWIIFARDMIINPTQEIIIQSNKNLYGRRAQVHIGKRGGLTIQ 613
Score = 105 bits (225), Expect = 4e-022
Identities = 48/75 (64%), Positives = 49/75 (65%)
Frame = -2 / -3
Query: 252 AKVIHSGSIWMTPHRSVPGRGFXXXXXXXXXXX*NLPASPPVVLRPNPLAHRARRFRSVA 73
AK+IH GS W TPHRSVPGRG * LP PPV RP PLA RA R RSVA
Sbjct: 501 AKMIHKGSSWTTPHRSVPGRGLVKSSSLGSVTM*ILPWRPPVAPRPKPLAQRASRCRSVA 322
Query: 72 QSCRHRQHRSIGFVA 28
QS R RQHRSIGFVA
Sbjct: 321 QSLRQRQHRSIGFVA 277
>gb|AV915392.1|AV915392 AV915392 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags14d11 5', mRNA sequence
Length = 554
Score = 167 bits (360), Expect = 9e-041
Identities = 64/123 (52%), Positives = 85/123 (69%)
Frame = +2 / +3
Query: 722 MPRCRWGFFHVVNNDYTHWLMYAIGGSKAPTIISQGNRYIAPPNLAAKQVTKQHDTPESV 901
MPRCR G+FHVVNNDYTHW MYAIGGS PTI SQGNRY+AP N AK+VTK+ +T ++
Sbjct: 21 MPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNRYLAPTNPFAKEVTKRVETAQTT 200
Query: 902 WKNWVWHSENDLFMEGAYFTVTGGQINRQFNKKDLIKPRNGSYVTRLTRYAGSLACTPGK 1081
WK W W SE D+ + GA+FT +G + +++ + ++ S V +T AG+L+C G
Sbjct: 201 WKAWNWRSEGDMLLNGAFFTPSGAGASASYSRASSLGAKSSSMVATITSGAGALSCHKGS 380
Query: 1082 PC* 1090
C*
Sbjct: 381 SC* 389
>gb|CA001634.1|CA001634 HS05C08r HS Hordeum vulgare subsp. vulgare cDNA clone HS05C08
5-PRIME, mRNA sequence
Length = 521
Score = 162 bits (348), Expect = 4e-039
Identities = 62/121 (51%), Positives = 83/121 (68%)
Frame = +2 / +1
Query: 728 RCRWGFFHVVNNDYTHWLMYAIGGSKAPTIISQGNRYIAPPNLAAKQVTKQHDTPESVWK 907
RCR G+FHVVNNDYTHW MYAIGGS PTI SQGNRY+AP N AK+VTK+ +T ++ WK
Sbjct: 13 RCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNRYLAPTNPFAKEVTKRVETAQTTWK 192
Query: 908 NWVWHSENDLFMEGAYFTVTGGQINRQFNKKDLIKPRNGSYVTRLTRYAGSLACTPGKPC 1087
W W SE D+ + GA+FT +G + +++ + ++ S V +T AG+L+C G C
Sbjct: 193 AWNWRSEGDMLLNGAFFTPSGAGASASYSRASSLGAKSSSMVATITSGAGALSCHKGSSC 372
Query: 1088 * 1090
*
Sbjct: 373 * 375
>gb|CB881433.1|CB881433 HM09M17w HM Hordeum vulgare subsp. vulgare cDNA clone HM09M17
3-PRIME, mRNA sequence
Length = 490
Score = 133 bits (286), Expect = 1e-030
Identities = 53/101 (52%), Positives = 72/101 (71%)
Frame = +2 / -1
Query: 788 AIGGSKAPTIISQGNRYIAPPNLAAKQVTKQHDTPESVWKNWVWHSENDLFMEGAYFTVT 967
AIGG+ PTIISQGNR+IAP + AK+VTK+ TP + +K+WVW S+ D+ M GA+F +
Sbjct: 490 AIGGNPDPTIISQGNRFIAPDDPNAKEVTKREYTPYNEYKDWVWKSQGDVMMNGAFFNQS 311
Query: 968 GGQINRQFNKKDLIKPRNGSYVTRLTRYAGSLACTPGKPC* 1090
GGQ R + D I ++G YV +LT++AG+L C GKPC*
Sbjct: 310 GGQNERTYGNMDFIPAKHGKYVGQLTQFAGTLECRVGKPC* 188
>gb|BQ764302.1|BQ764302 EBan01_SQ005_P07_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ005_P07 5', mRNA sequence
Length = 450
Score = 105 bits (225), Expect = 4e-022
Identities = 40/59 (67%), Positives = 42/59 (71%)
Frame = +2 / +3
Query: 23 CRATNPIDRCWRCRQDWATDRKRLARCAKGFGRNTTGGLAGKFYXXXXXXXXXXXNPRP 199
C ATNPIDRCWRCR+DWATDR+RLARCAKGF R TGGL GK Y NPRP
Sbjct: 273 CLATNPIDRCWRCRKDWATDRQRLARCAKGFCRGATGGLHGKIYIVTDPSDEDFTNPRP 449
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 442,249,658
Number of Sequences: 312970
Number of extensions: 7129332
Number of successful extensions: 55311
Number of sequences better than 1.0e-020: 13
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 15462584530
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)