TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3148257.2.1
(675 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV927733.1|AV927733 AV927733 K. Sato unpublished cDNA li... 127 6e-029
gb|AV934689.1|AV934689 AV934689 K. Sato unpublished cDNA li... 127 6e-029
gb|AV936387.1|AV936387 AV936387 K. Sato unpublished cDNA li... 127 6e-029
gb|BJ473617.1|BJ473617 BJ473617 K. Sato unpublished cDNA li... 127 6e-029
gb|BJ475011.1|BJ475011 BJ475011 K. Sato unpublished cDNA li... 127 6e-029
gb|BJ477252.1|BJ477252 BJ477252 K. Sato unpublished cDNA li... 127 6e-029
gb|BQ661605.1|BQ661605 HM04E05u HM Hordeum vulgare subsp. v... 127 6e-029
gb|BU972175.1|BU972175 HB20N14r BC Hordeum vulgare subsp. v... 122 3e-027
gb|AV837078.1|AV837078 AV837078 K. Sato unpublished cDNA li... 121 4e-027
gb|BG415664.1|BG415664 HVSMEk0007G03f Hordeum vulgare testa... 121 4e-027
gb|BG417338.1|BG417338 HVSMEk0017J08f Hordeum vulgare testa... 121 4e-027
gb|AY177665.1| Hordeum vulgare subsp. vulgare putative call... 121 4e-027
gb|AV931607.1|AV931607 AV931607 K. Sato unpublished cDNA li... 120 1e-026
gb|BG418775.1|BG418775 HVSMEk0024G07f Hordeum vulgare testa... 119 2e-026
gb|BM377017.2|BM377017 EBem05_SQ003_O02_R embryo, 14 DPA, n... 116 2e-025
gb|CD663772.1|CD663772 UCRHV18_06cd06_b1 Drought-stressed D... 111 6e-024
gb|CB882058.1|CB882058 HM11L21w HM Hordeum vulgare subsp. v... 110 8e-024
gb|BU995906.1|BU995906 HM11L21r HM Hordeum vulgare subsp. v... 105 3e-022
gb|CD662722.1|CD662722 UCRHV18_02df07_b1 Drought-stressed D... 105 3e-022
>gb|AV927733.1|AV927733 AV927733 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd3g22 3', mRNA sequence
Length = 626
Score = 127 bits (272), Expect = 6e-029
Identities = 52/103 (50%), Positives = 71/103 (68%)
Frame = +3 / -3
Query: 129 IIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAPVALL 308
I DIF +LAF+PTGWGL+ +AQ ++P I +W SI ++AR YEI++G+ + P+A L
Sbjct: 519 IQDIFVCILAFMPTGWGLLLVAQALKPAIMRVGLWGSIRALARGYEIIMGLLLFTPIAFL 340
Query: 309 SWLPGFQEMQTRVLFNEGFSRGLQISRILTGKRTNTV*GSDPD 437
+W P E QTR+LFN+ FSRGLQISRIL G + + S D
Sbjct: 339 AWFPFVSEFQTRMLFNQAFSRGLQISRILGGHKKDRAARSKDD 211
>gb|AV934689.1|AV934689 AV934689 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal1e06 3', mRNA sequence
Length = 678
Score = 127 bits (272), Expect = 6e-029
Identities = 52/103 (50%), Positives = 71/103 (68%)
Frame = +3 / -1
Query: 129 IIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAPVALL 308
I DIF +LAF+PTGWGL+ +AQ ++P I +W SI ++AR YEI++G+ + P+A L
Sbjct: 573 IQDIFVCILAFMPTGWGLLLVAQALKPAIMRVGLWGSIRALARGYEIIMGLLLFTPIAFL 394
Query: 309 SWLPGFQEMQTRVLFNEGFSRGLQISRILTGKRTNTV*GSDPD 437
+W P E QTR+LFN+ FSRGLQISRIL G + + S D
Sbjct: 393 AWFPFVSEFQTRMLFNQAFSRGLQISRILGGHKKDRAARSKDD 265
>gb|AV936387.1|AV936387 AV936387 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal11c09 3', mRNA sequence
Length = 650
Score = 127 bits (272), Expect = 6e-029
Identities = 52/103 (50%), Positives = 71/103 (68%)
Frame = +3 / -3
Query: 129 IIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAPVALL 308
I DIF +LAF+PTGWGL+ +AQ ++P I +W SI ++AR YEI++G+ + P+A L
Sbjct: 543 IQDIFVCILAFMPTGWGLLLVAQALKPAIMRVGLWGSIRALARGYEIIMGLLLFTPIAFL 364
Query: 309 SWLPGFQEMQTRVLFNEGFSRGLQISRILTGKRTNTV*GSDPD 437
+W P E QTR+LFN+ FSRGLQISRIL G + + S D
Sbjct: 363 AWFPFVSEFQTRMLFNQAFSRGLQISRILGGHKKDRAARSKDD 235
>gb|BJ473617.1|BJ473617 BJ473617 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal15l17 3', mRNA sequence
Length = 633
Score = 127 bits (272), Expect = 6e-029
Identities = 52/103 (50%), Positives = 71/103 (68%)
Frame = +3 / -2
Query: 129 IIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAPVALL 308
I DIF +LAF+PTGWGL+ +AQ ++P I +W SI ++AR YEI++G+ + P+A L
Sbjct: 527 IQDIFVCILAFMPTGWGLLLVAQALKPAIMRVGLWGSIRALARGYEIIMGLLLFTPIAFL 348
Query: 309 SWLPGFQEMQTRVLFNEGFSRGLQISRILTGKRTNTV*GSDPD 437
+W P E QTR+LFN+ FSRGLQISRIL G + + S D
Sbjct: 347 AWFPFVSEFQTRMLFNQAFSRGLQISRILGGHKKDRAARSKDD 219
>gb|BJ475011.1|BJ475011 BJ475011 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal27j14 3', mRNA sequence
Length = 623
Score = 127 bits (272), Expect = 6e-029
Identities = 52/103 (50%), Positives = 71/103 (68%)
Frame = +3 / -1
Query: 129 IIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAPVALL 308
I DIF +LAF+PTGWGL+ +AQ ++P I +W SI ++AR YEI++G+ + P+A L
Sbjct: 521 IQDIFVCILAFMPTGWGLLLVAQALKPAIMRVGLWGSIRALARGYEIIMGLLLFTPIAFL 342
Query: 309 SWLPGFQEMQTRVLFNEGFSRGLQISRILTGKRTNTV*GSDPD 437
+W P E QTR+LFN+ FSRGLQISRIL G + + S D
Sbjct: 341 AWFPFVSEFQTRMLFNQAFSRGLQISRILGGHKKDRAARSKDD 213
>gb|BJ477252.1|BJ477252 BJ477252 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal40n15 3', mRNA sequence
Length = 623
Score = 127 bits (272), Expect = 6e-029
Identities = 52/103 (50%), Positives = 71/103 (68%)
Frame = +3 / -1
Query: 129 IIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAPVALL 308
I DIF +LAF+PTGWGL+ +AQ ++P I +W SI ++AR YEI++G+ + P+A L
Sbjct: 521 IQDIFVCILAFMPTGWGLLLVAQALKPAIMRVGLWGSIRALARGYEIIMGLLLFTPIAFL 342
Query: 309 SWLPGFQEMQTRVLFNEGFSRGLQISRILTGKRTNTV*GSDPD 437
+W P E QTR+LFN+ FSRGLQISRIL G + + S D
Sbjct: 341 AWFPFVSEFQTRMLFNQAFSRGLQISRILGGHKKDRAARSKDD 213
>gb|BQ661605.1|BQ661605 HM04E05u HM Hordeum vulgare subsp. vulgare cDNA clone HM04E05
3-PRIME, mRNA sequence
Length = 616
Score = 127 bits (272), Expect = 6e-029
Identities = 52/103 (50%), Positives = 71/103 (68%)
Frame = +3 / -1
Query: 129 IIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAPVALL 308
I DIF +LAF+PTGWGL+ +AQ ++P I +W SI ++AR YEI++G+ + P+A L
Sbjct: 583 IQDIFVCILAFMPTGWGLLLVAQALKPAIMRVGLWGSIRALARGYEIIMGLLLFTPIAFL 404
Query: 309 SWLPGFQEMQTRVLFNEGFSRGLQISRILTGKRTNTV*GSDPD 437
+W P E QTR+LFN+ FSRGLQISRIL G + + S D
Sbjct: 403 AWFPFVSEFQTRMLFNQAFSRGLQISRILGGHKKDRAARSKDD 275
>gb|BU972175.1|BU972175 HB20N14r BC Hordeum vulgare subsp. vulgare cDNA clone HB20N14
5-PRIME, mRNA sequence
Length = 373
Score = 122 bits (260), Expect = 3e-027
Identities = 47/93 (50%), Positives = 65/93 (69%)
Frame = +3 / +3
Query: 129 IIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAPVALL 308
I DI LAF+PTGWG++ IAQ P T +W S+ ++AR YE+++G+ + P+ +L
Sbjct: 27 IKDILVCFLAFLPTGWGILLIAQPCIPLFRVTGLWGSVRALARAYEVIMGMLLFTPITVL 206
Query: 309 SWLPGFQEMQTRVLFNEGFSRGLQISRILTGKR 407
SW P E QTR+LFN+ FSRGLQISRIL G++
Sbjct: 207 SWFPFVSEFQTRMLFNQAFSRGLQISRILGGQK 305
>gb|AV837078.1|AV837078 AV837078 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd19k01, mRNA
sequence
Length = 618
Score = 121 bits (259), Expect = 4e-027
Identities = 44/99 (44%), Positives = 67/99 (67%)
Frame = +3 / -3
Query: 117 TEFQIIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAP 296
T ++D+ S+LA++PTGWG++SIA +P ++ +W ++ S+ARLY+ +G+ I P
Sbjct: 607 TRLAVVDVLASILAYVPTGWGILSIAVAWKPIVKRLGLWKTVRSLARLYDAGMGMIIFVP 428
Query: 297 VALLSWLPGFQEMQTRVLFNEGFSRGLQISRILTGKRTN 413
+A+ SW P QTR+LFN+ FSRGL+IS IL G N
Sbjct: 427 IAICSWFPFISTFQTRLLFNQAFSRGLEISLILAGNNQN 311
>gb|BG415664.1|BG415664 HVSMEk0007G03f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0007G03f, mRNA sequence
Length = 641
Score = 121 bits (259), Expect = 4e-027
Identities = 44/99 (44%), Positives = 67/99 (67%)
Frame = +3 / -2
Query: 117 TEFQIIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAP 296
T ++D+ S+LA++PTGWG++SIA +P ++ +W ++ S+ARLY+ +G+ I P
Sbjct: 520 TRLAVVDVLASILAYVPTGWGILSIAVAWKPIVKRLGLWKTVRSLARLYDAGMGMIIFVP 341
Query: 297 VALLSWLPGFQEMQTRVLFNEGFSRGLQISRILTGKRTN 413
+A+ SW P QTR+LFN+ FSRGL+IS IL G N
Sbjct: 340 IAICSWFPFISTFQTRLLFNQAFSRGLEISLILAGNNQN 224
>gb|BG417338.1|BG417338 HVSMEk0017J08f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0017J08f, mRNA sequence
Length = 839
Score = 121 bits (259), Expect = 4e-027
Identities = 44/99 (44%), Positives = 67/99 (67%)
Frame = +3 / +2
Query: 117 TEFQIIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAP 296
T ++D+ S+LA++PTGWG++SIA +P ++ +W ++ S+ARLY+ +G+ I P
Sbjct: 140 TRLAVVDVLASILAYVPTGWGILSIAVAWKPIVKRLGLWKTVRSLARLYDAGMGMIIFVP 319
Query: 297 VALLSWLPGFQEMQTRVLFNEGFSRGLQISRILTGKRTN 413
+A+ SW P QTR+LFN+ FSRGL+IS IL G N
Sbjct: 320 IAICSWFPFISTFQTRLLFNQAFSRGLEISLILAGNNQN 436
>gb|AY177665.1| Hordeum vulgare subsp. vulgare putative callose synthase mRNA,
complete cds
Length = 6180
Score = 121 bits (259), Expect = 4e-027
Identities = 44/99 (44%), Positives = 67/99 (67%)
Frame = +3 / +3
Query: 117 TEFQIIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAP 296
T ++D+ S+LA++PTGWG++SIA +P ++ +W ++ S+ARLY+ +G+ I P
Sbjct: 5532 TRLAVVDVLASILAYVPTGWGILSIAVAWKPIVKRLGLWKTVRSLARLYDAGMGMIIFVP 5711
Query: 297 VALLSWLPGFQEMQTRVLFNEGFSRGLQISRILTGKRTN 413
+A+ SW P QTR+LFN+ FSRGL+IS IL G N
Sbjct: 5712 IAICSWFPFISTFQTRLLFNQAFSRGLEISLILAGNNQN 5828
>gb|AV931607.1|AV931607 AV931607 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd23h13 3', mRNA sequence
Length = 508
Score = 120 bits (256), Expect = 1e-026
Identities = 48/96 (50%), Positives = 67/96 (69%)
Frame = +3 / -2
Query: 150 LLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAPVALLSWLPGFQ 329
+LAF+PTGWGL+ +AQ ++P I +W SI ++AR YEI++G+ + P+A L+W P
Sbjct: 501 ILAFMPTGWGLLLVAQALKPAIMRVGLWGSIRALARGYEIIMGLLLFTPIAFLAWFPFVS 322
Query: 330 EMQTRVLFNEGFSRGLQISRILTGKRTNTV*GSDPD 437
E QTR+LFN+ FSRGLQISRIL G + + S D
Sbjct: 321 EFQTRMLFNQAFSRGLQISRILGGHKKDRAARSKDD 214
>gb|BG418775.1|BG418775 HVSMEk0024G07f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0024G07f, mRNA sequence
Length = 834
Score = 119 bits (254), Expect = 2e-026
Identities = 43/95 (45%), Positives = 66/95 (69%)
Frame = +3 / +2
Query: 117 TEFQIIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAP 296
T ++D+ S+LA++PTGWG++SIA +P ++ +W ++ S+ARLY+ +G+ I P
Sbjct: 362 TRLAVVDVLASILAYVPTGWGILSIAVAWKPIVKRLGLWKTVRSLARLYDAGMGMIIFVP 541
Query: 297 VALLSWLPGFQEMQTRVLFNEGFSRGLQISRILTG 401
+A+ SW P QTR+LFN+ FSRGL+IS IL G
Sbjct: 542 IAICSWFPFISTFQTRLLFNQAFSRGLEISLILAG 646
>gb|BM377017.2|BM377017 EBem05_SQ003_O02_R embryo, 14 DPA, no treatment, cv Optic, EBem05
Hordeum vulgare subsp. vulgare cDNA clone
EBem05_SQ003_O02 5', mRNA sequence
Length = 466
Score = 116 bits (247), Expect = 2e-025
Identities = 44/85 (51%), Positives = 62/85 (72%)
Frame = +3 / +1
Query: 129 IIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAPVALL 308
I DIF +LAF+PTGWGL+ +AQ ++P I +W SI ++AR Y+I++G+ + P+A L
Sbjct: 211 IQDIFVCILAFMPTGWGLLLVAQALKPAIMKVGLWGSIRALARGYQIIMGLLLFTPIAFL 390
Query: 309 SWLPGFQEMQTRVLFNEGFSRGLQI 383
+W P E QTR+LFN+ FSRGLQI
Sbjct: 391 AWFPFVSEFQTRMLFNQAFSRGLQI 465
>gb|CD663772.1|CD663772 UCRHV18_06cd06_b1 Drought-stressed Dicktoo barley epidermis cDNA
library Hordeum vulgare subsp. vulgare cDNA clone
UCRHV18_06cd06, mRNA sequence
Length = 639
Score = 111 bits (236), Expect = 6e-024
Identities = 41/94 (43%), Positives = 62/94 (65%)
Frame = +3 / -1
Query: 132 IDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAPVALLS 311
+D+ S+LA++PTGWG+ SIA +P ++ +W ++ S+A LY+ +G+ I P+A+ S
Sbjct: 630 VDVLASILAYVPTGWGIFSIAVAWKPIVKRLGLWKTVRSLAPLYDAGMGMIIFVPIAICS 451
Query: 312 WLPGFQEMQTRVLFNEGFSRGLQISRILTGKRTN 413
W P QTR+LFN+ FSRGL+I IL G N
Sbjct: 450 WFPFISPFQTRLLFNQAFSRGLEIFFILAGNNQN 349
>gb|CB882058.1|CB882058 HM11L21w HM Hordeum vulgare subsp. vulgare cDNA clone HM11L21
3-PRIME, mRNA sequence
Length = 585
Score = 110 bits (235), Expect = 8e-024
Identities = 41/99 (41%), Positives = 64/99 (64%)
Frame = +3 / -1
Query: 117 TEFQIIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAP 296
T+F I D+F S LAF+ TGW ++ +A + +++ +WDS+ ++R+Y+ +G I AP
Sbjct: 585 TDFTIADLFASALAFLATGWCILCLAITWKRVVKTLGLWDSVREISRMYDAGMGAVIFAP 406
Query: 297 VALLSWLPGFQEMQTRVLFNEGFSRGLQISRILTGKRTN 413
+ SW P Q+R+LFN+ FSRGL+IS IL G + N
Sbjct: 405 IVFFSWFPFVSTFQSRILFNQAFSRGLEISLILAGNKAN 289
>gb|BU995906.1|BU995906 HM11L21r HM Hordeum vulgare subsp. vulgare cDNA clone HM11L21
5-PRIME, mRNA sequence
Length = 641
Score = 105 bits (223), Expect = 3e-022
Identities = 39/95 (41%), Positives = 61/95 (64%)
Frame = +3 / +3
Query: 129 IIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAPVALL 308
I D+F S LAF+ TGW ++ +A + +++ +WDS+ ++R+Y+ +G I AP+
Sbjct: 72 IADLFASALAFLATGWCILCLAITWKRVVKTLGLWDSVREISRMYDAGMGAVIFAPIVFF 251
Query: 309 SWLPGFQEMQTRVLFNEGFSRGLQISRILTGKRTN 413
SW P Q+R+LFN+ FSRGL+IS IL G + N
Sbjct: 252 SWFPFVSTFQSRILFNQAFSRGLEISLILAGNKAN 356
>gb|CD662722.1|CD662722 UCRHV18_02df07_b1 Drought-stressed Dicktoo barley epidermis cDNA
library Hordeum vulgare subsp. vulgare cDNA clone
UCRHV18_02df07, mRNA sequence
Length = 676
Score = 105 bits (223), Expect = 3e-022
Identities = 39/95 (41%), Positives = 61/95 (64%)
Frame = +3 / -2
Query: 129 IIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWDSIISVARLYEILLGVFIMAPVALL 308
I D+F S LAF+ TGW ++ +A + +++ +WDS+ ++R+Y+ +G I AP+
Sbjct: 666 IADLFASALAFLATGWCILCLAITWKRVVKTLGLWDSVREISRMYDAGMGAVIFAPIVFF 487
Query: 309 SWLPGFQEMQTRVLFNEGFSRGLQISRILTGKRTN 413
SW P Q+R+LFN+ FSRGL+IS IL G + N
Sbjct: 486 SWFPFVSTFQSRILFNQAFSRGLEISLILAGNKAN 382
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 292,323,065
Number of Sequences: 312970
Number of extensions: 4174382
Number of successful extensions: 34377
Number of sequences better than 1.0e-020: 23
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7338090657
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)