TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115208.2.3
(672 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AL505038.1|AL505038 AL505038 Hordeum vulgare Barke roots... 136 1e-038
gb|CK125352.1|CK125352 BES1824107m07 BES1824 Hordeum vulgar... 131 5e-030
gb|AJ461534.1|AJ461534 AJ461534 S00002 Hordeum vulgare subs... 124 4e-028
gb|BE420880.1|BE420880 HWM003.E05 ITEC HWM Barley Leaf Libr... 113 1e-024
gb|AJ462026.1|AJ462026 AJ462026 S00002 Hordeum vulgare subs... 108 4e-023
>gb|AL505038.1|AL505038 AL505038 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW07D06V 5', mRNA sequence
Length = 694
Score = 136 bits (291), Expect(2) = 1e-038
Identities = 49/100 (49%), Positives = 70/100 (70%)
Frame = +2 / +3
Query: 20 GPLHVIPNVITAEFRTFIEIVFENPEKSIDSLHVDGYAFFGVGMGPGKWSPEVRKTYNLL 199
GP ++ +V+ + R ++E+VFEN E + S H+DGYAF+ VGM G+W+P R+ YNL
Sbjct: 36 GPAYLQTSVMASNMRDYVEVVFENAEGYVQSWHIDGYAFWVVGMDGGQWTPASRQNYNLR 215
Query: 200 DTVSRHTIQVYPRSWTAIMLTFDNAGMWSVHSNIWERYYL 319
D ++R+T+QVYP +WTAI + DN GMW+V S W R YL
Sbjct: 216 DAIARYTLQVYPGAWTAIYMPLDNVGMWNVRSESWGRQYL 335
Score = 93.2 bits (197), Expect(2) = 8e-022
Identities = 39/72 (54%), Positives = 47/72 (65%)
Frame = -3 / -3
Query: 319 EVVPLPDVGMHAPHARVVERQHDCRP*PRVHLDRVPAHRVQQVVRLPHLRRPLPGAHPDA 140
+V+P P + H PH VV+R D RP R+HL RVP RV QVV L RRPLP HPD
Sbjct: 335 QVLPAPALRPHVPHPHVVQRHVDRRPRSRIHLQRVPGDRVPQVVVLTARRRPLPSVHPDD 156
Query: 139 EEGVAIDVEGVY 104
EGVA+DV G++
Sbjct: 155 PEGVAVDVPGLH 120
Score = 43.2 bits (88), Expect(2) = 1e-038
Identities = 19/41 (46%), Positives = 24/41 (58%)
Frame = +2 / +2
Query: 323 EQFYISVVSPARSLRDEYNMPDNALRCGKVVGLPLPPSYAP 445
+QFY+ V SPA S R E +P NAL CG+ G P +P
Sbjct: 338 QQFYLRVYSPANSWRXENPIPKNALLCGRASGRRTRPF*SP 460
Score = 29.9 bits (59), Expect(2) = 8e-022
Identities = 14/27 (51%), Positives = 16/27 (59%)
Frame = -1 / -2
Query: 405 PQRRALSGMLYSSRSDRAGETTLM*NC 325
PQ A GM +S R + AGE T *NC
Sbjct: 420 PQSSAFLGMGFSXRHELAGE*TRR*NC 340
>gb|CK125352.1|CK125352 BES1824107m07 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010M077 5-PRIME, mRNA sequence
Length = 930
Score = 131 bits (280), Expect = 5e-030
Identities = 47/122 (38%), Positives = 76/122 (62%)
Frame = +2 / +2
Query: 41 NVITAEFRTFIEIVFENPEKSIDSLHVDGYAFFGVGMGPGKWSPEVRKTYNLLDTVSRHT 220
+V+ A ++ F+E+VF+N E + + H+DGY+FF VGM G+W+P R +YN D +SR T
Sbjct: 455 SVLNASYKGFLEMVFQNNETDVQTYHLDGYSFFVVGMDYGEWTPNSRGSYNKWDAISRST 634
Query: 221 IQVYPRSWTAIMLTFDNAGMWSVHSNIWERYYLGEQFYISVVSPARSLRDEYNMPDNALR 400
QV+P WTA++++ DN G+W++ + + +Y G++ Y+ V P E P L
Sbjct: 635 TQVFPGGWTAVLVSLDNVGIWNIRAEKLDNWYNGQEVYVKVADPLGYNVTEMIAPKXTLY 814
Query: 401 CG 406
CG
Sbjct: 815 CG 820
>gb|AJ461534.1|AJ461534 AJ461534 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200012C08F1, mRNA sequence
Length = 300
Score = 124 bits (266), Expect = 4e-028
Identities = 43/89 (48%), Positives = 65/89 (73%)
Frame = +2 / +1
Query: 158 GKWSPEVRKTYNLLDTVSRHTIQVYPRSWTAIMLTFDNAGMWSVHSNIWERYYLGEQFYI 337
G+W+ R YNL+D +RHT+QVYP W+AI+++ DN GMW++ S W+R+YLG+Q Y+
Sbjct: 13 GQWTENERLNYNLVDAQARHTVQVYPNGWSAILVSLDNQGMWNLRSANWDRHYLGQQLYL 192
Query: 338 SVVSPARSLRDEYNMPDNALRCGKVVGLP 424
V + +SL +EY++P NA+ CG+ GLP
Sbjct: 193 RVWTAEQSLSNEYSIPTNAILCGRADGLP 279
>gb|BE420880.1|BE420880 HWM003.E05 ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM003.E05, mRNA sequence
Length = 890
Score = 113 bits (241), Expect = 1e-024
Identities = 45/112 (40%), Positives = 70/112 (62%)
Frame = +2 / +2
Query: 17 NGPLHVIPNVITAEFRTFIEIVFENPEKSIDSLHVDGYAFFGVGMGPGKWSPEVRKTYNL 196
+GP + +VI + ++ F+EIVF+N + + + H+DGYAF+ VGM G+W+ R TYN
Sbjct: 344 DGPPVIGSSVINSTYKNFMEIVFQNNDTKVQTYHIDGYAFWVVGMDYGEWTDSSRGTYNK 523
Query: 197 LDTVSRHTIQVYPRSWTAIMLTFDNAGMWSVHSNIWERYYLGEQFYISVVSP 352
D VSR T QV+P +WT +L D+ W+V + +YL ++ Y+ VV P
Sbjct: 524 WDGVSRCTTQVFPGAWTGGLLPLDSPAFWNVRTENLGPWYLWQETYLRVVEP 679
>gb|AJ462026.1|AJ462026 AJ462026 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200079D08F1, mRNA sequence
Length = 300
Score = 108 bits (230), Expect = 4e-023
Identities = 42/80 (52%), Positives = 54/80 (67%)
Frame = +2 / +1
Query: 206 VSRHTIQVYPRSWTAIMLTFDNAGMWSVHSNIWERYYLGEQFYISVVSPARSLRDEYNMP 385
++R+T+QVYP +WTAI + DN GMW+V S W R YLG+QFY+ V SPA S RDE +P
Sbjct: 1 IARYTLQVYPGAWTAIYMPLDNVGMWNVRSESWGRQYLGQQFYLRVYSPANSWRDENPIP 180
Query: 386 DNALRCGKVVGLPLPPSYAP 445
NAL CG+ G P +P
Sbjct: 181 KNALLCGRASGRRTRPL*SP 240
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 294,080,475
Number of Sequences: 312970
Number of extensions: 4541329
Number of successful extensions: 81435
Number of sequences better than 1.0e-020: 8
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7295673948
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)