TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3106097.2.2
(1005 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG416908.2|BG416908 HVSMEk0015C11f Hordeum vulgare testa... 344 6e-094
gb|BQ767300.1|BQ767300 EBro08_SQ008_C18_R root, 3 week, dro... 337 6e-092
gb|CA015292.1|CA015292 HT13N20r HT Hordeum vulgare subsp. v... 309 2e-083
gb|AF261107.1|AF261107 Hordeum vulgare sucrose-phosphate sy... 220 2e-069
gb|CD662445.1|CD662445 UCRHV18_01cb12_b1 Drought-stressed D... 211 2e-068
gb|BJ466481.1|BJ466481 BJ466481 K. Sato unpublished cDNA li... 212 7e-066
gb|BJ468331.1|BJ468331 BJ468331 K. Sato unpublished cDNA li... 247 7e-065
gb|BI950175.1|BI950175 HVSMEl0019E11f Hordeum vulgare spike... 245 3e-064
gb|BE601555.2|BE601555 HVSMEh0098G14f Hordeum vulgare 5-45 ... 239 2e-062
gb|BE437516.1|BE437516 SFR004.E11F990621 ITEC SFR Barley Le... 186 4e-062
gb|BU984860.1|BU984860 HF05E11r HF Hordeum vulgare subsp. v... 195 5e-061
gb|AV917170.1|AV917170 AV917170 K. Sato unpublished cDNA li... 219 2e-056
gb|CA022248.1|CA022248 HZ42J07r HZ Hordeum vulgare subsp. v... 211 4e-054
gb|AV931912.1|AV931912 AV931912 K. Sato unpublished cDNA li... 177 4e-054
gb|AV914348.1|AV914348 AV914348 K. Sato unpublished cDNA li... 199 3e-053
gb|BG418110.1|BG418110 HVSMEk0021F18f Hordeum vulgare testa... 193 1e-048
gb|CA011510.1|CA011510 HT05E02u HT Hordeum vulgare subsp. v... 184 8e-046
gb|BQ768491.1|BQ768491 EBro08_SQ011_I24_R root, 3 week, dro... 152 3e-036
gb|AV834625.1|AV834625 AV834625 K. Sato unpublished cDNA li... 150 1e-035
gb|BM444184.1|BM444184 EBem09_SQ004_G12_R embryo, 1 Day ger... 145 4e-034
gb|BU989662.1|BU989662 HF22F18r HF Hordeum vulgare subsp. v... 135 3e-031
gb|BU990273.1|BU990273 HF24J09r HF Hordeum vulgare subsp. v... 135 3e-031
gb|CB859330.1|CB859330 HI10C07w HI Hordeum vulgare subsp. v... 135 3e-031
gb|BU994139.1|BU994139 HM05O02r HM Hordeum vulgare subsp. v... 126 2e-028
gb|BM442430.2|BM442430 EBan01_SQ003_C15_R anther, yellow st... 125 5e-028
gb|CA016632.1|CA016632 HV08B18u HV Hordeum vulgare subsp. v... 117 8e-026
gb|BJ454449.1|BJ454449 BJ454449 K. Sato unpublished cDNA li... 117 1e-025
gb|CB874869.1|CB874869 HX06J01w HX Hordeum vulgare subsp. v... 115 5e-025
gb|CB880803.1|CB880803 HM07O02w HM Hordeum vulgare subsp. v... 111 5e-024
gb|AL503268.1|AL503268 AL503268 Hordeum vulgare Barke roots... 55 2e-023
>gb|BG416908.2|BG416908 HVSMEk0015C11f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0015C11f, mRNA sequence
Length = 488
Score = 344 bits (745), Expect = 6e-094
Identities = 139/162 (85%), Positives = 150/162 (92%)
Frame = +3 / +3
Query: 63 PSSSSEDMLNPAELPFMIDLDYHSQIEYRWGGEGLRKTLIRWAAEKNKERGQKIFIEDEE 242
PSS+SEDML+PAELPFMIDLDYHSQI+YRWGGEGLRKTLIRWAAEKN ERGQ+ ED+E
Sbjct: 3 PSSNSEDMLSPAELPFMIDLDYHSQIQYRWGGEGLRKTLIRWAAEKNSERGQEAVTEDDE 182
Query: 243 CSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSKLNVIPVLASRSQALRY 422
CSSTYCISFK NT A PPVK++R+TMRIQALRCHVLYSHDGSKLN IPVLASRSQALRY
Sbjct: 183 CSSTYCISFKVKNTEAVPPVKDLRKTMRIQALRCHVLYSHDGSKLNFIPVLASRSQALRY 362
Query: 423 LYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGSFN 548
LYIRWGVELS +TV+VGE GDTDYEGLLGG+ KTIILKGSFN
Sbjct: 363 LYIRWGVELSKMTVVVGESGDTDYEGLLGGMQKTIILKGSFN 488
Score = 230 bits (497), Expect(2) = 3e-061
Identities = 104/136 (76%), Positives = 114/136 (83%)
Frame = -3 / -2
Query: 469 TITVMFDSSTPHRIYKYLKACEREAKTGITFNLLPSWLYKTWQRSACILIVLLISFTGGA 290
T TV+FDSSTPH IYKYL A +R+A+TG+ FNLLPSWLY T R+A ILIV L SFTGG
Sbjct: 409 TTTVIFDSSTPHLIYKYLSAWDRDARTGMKFNLLPSWLYNT*HRNA*ILIVFLRSFTGGT 230
Query: 289 AAVLDPLNEMQ*VDEHSSSSINIFCPLSLFFSAAQRINVFLKPSPPQRYSIWE**SRSIM 110
A+V L EMQ*VDEHSSSS+ CPLSLFFSAAQRI+VFLKPSPPQRY I E**SRSI+
Sbjct: 229 ASVFFTLKEMQ*VDEHSSSSVTASCPLSLFFSAAQRISVFLKPSPPQRY*I*E**SRSII 50
Query: 109 NGSSAGLSMSSELEDG 62
NGSSAGLSMSSE EDG
Sbjct: 49 NGSSAGLSMSSEFEDG 2
Score = 213 bits (459), Expect = 2e-054
Identities = 97/162 (59%), Positives = 111/162 (68%)
Frame = -2 / -1
Query: 548 IERAFEYDSFVHASK*SFIICVTTLTDNHGDV*QLYPPSDIQIP*SL*ARSQNRNYIQLA 369
I+ AFEYD +H SK* FIIC+ T+NH +*QLYPP IQ+P* L + NR +QLA
Sbjct: 488 IK*AFEYDGLLHPSK*PFIICIAAFTNNHRHL*QLYPPPYIQVP*CLGS*C*NRYEVQLA 309
Query: 368 TIMAVQNMATQCLYSHCPPNLLHRRCSCSIGPFK*NAVGG*AFFILNKYFLSPFFVFLSC 189
IMAVQ M QCL SHC +LHRR SI FK NAV G* F I + FLSP + C
Sbjct: 308 AIMAVQYMTPQCLNSHCLSKILHRRDGLSILHFKRNAVSG*TFVIFSNCFLSPLAILFCC 129
Query: 188 PTN*CLP*TFSSPAIFNLGVIIKINHEWELSRVKHVLRA*RW 63
PTN*CLP*TFSSPAI NL VIIKI+H+W+L R KHV *RW
Sbjct: 128 PTN*CLP*TFSSPAILNLRVIIKIDHKWKLGRAKHVF*I*RW 3
Score = 171 bits (368), Expect = 6e-042
Identities = 81/136 (59%), Positives = 97/136 (71%)
Frame = +2 / +2
Query: 62 SIFKL*GHA*PC*APIHD*S*LSLPN*ISLGRRRFKEDINSLGS*EKQRKGTKNIY*G*R 241
SIFK HA*PC A I+D S*LSL + ISLGRRRFKED NSLGS ++ R+GT++ Y* *R
Sbjct: 2 SIFKFRRHA*PCRASIYDRS*LSLSDSISLGRRRFKEDTNSLGSRKE*REGTRSSY*R*R 181
Query: 242 MLIHLLHFI*RVQYCSCTSCEGD*EDNENTSTALPCFVQP*W*QVECNSCFGFSLTGFKV 421
M IHLLHF * +Y SCEG *+DNEN+S A+ C VQP*W QVE ++C ++ KV
Sbjct: 182 MFIHLLHFF*SEEY*GRPSCEGS*KDNENSSIAVSCIVQP*WQQVELHTCSSITIPSTKV 361
Query: 422 FVYPMGGRAVKHHRDC 469
VY +GGRAVK C
Sbjct: 362 LVYKVGGRAVKDDGGC 409
Score = 102 bits (218), Expect(3) = 5e-033
Identities = 48/74 (64%), Positives = 57/74 (77%)
Frame = -1 / -3
Query: 282 YWTL*MKCSRWMSILHPQ*IFFVPFLCFSQLPNELMSSLNLLLPSDIQFGSDNQDQS*MG 103
Y +L* KCS+WM+I H Q*+ VP LPNEL+SSLNLLLPSDI+ SDNQD+S*M
Sbjct: 222 YSSL*KKCSKWMNIRHLQ*LLLVPSRYSFLLPNELVSSLNLLLPSDIESESDNQDRS*ME 43
Query: 102 AQQG*ACPQSLKMD 61
A+QG*AC +LKMD
Sbjct: 42 ARQG*ACLLNLKMD 1
Score = 80.3 bits (169), Expect(2) = 2e-031
Identities = 40/72 (55%), Positives = 45/72 (62%)
Frame = +1 / +1
Query: 325 EYKHCVAMFCTAMMVAS*M*FLFWLLAHRL*GICISDGG*SCQTSP*LSVSVVTQIMKDY 504
E+KHC M+CTAMM AS* +LF + *G CI GG*SCQ L V QIMK Y
Sbjct: 265 EFKHCGVMYCTAMMAAS*TSYLF*HHDPKH*GTCI*GGG*SCQR*RWLLVKAAIQIMKGY 444
Query: 505 LEACTKLSYSKA 540
LE C + SYSKA
Sbjct: 445 LEGCRRPSYSKA 480
Score = 75.8 bits (159), Expect(2) = 2e-031
Identities = 32/46 (69%), Positives = 37/46 (80%)
Frame = +1 / +1
Query: 136 KLNIAGEEKV*GRH*FVGQLRKTKKGDKKYLLRMKNAHPPTAFHLK 273
+ NIAGEEKV*GRH*FVGQ ++ +GDKK LL+M N HP TAF LK
Sbjct: 76 RFNIAGEEKV*GRH*FVGQQKRIARGDKKQLLKMTNVHPLTAFLLK 213
Score = 46.4 bits (95), Expect(3) = 5e-033
Identities = 25/42 (59%), Positives = 27/42 (64%)
Frame = -1 / -3
Query: 540 SL*V**FCARLQVVLHNLCHHTHRQSR*CLTALPPIGYTNTL 415
SL*V* A LQV LHNL H+Q LTALPP YT+TL
Sbjct: 480 SL*V*WSSASLQVALHNLYRRFHQQPPSSLTALPPTLYTSTL 355
Score = 30.4 bits (60), Expect(3) = 5e-033
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = -1 / -3
Query: 378 STCYHHGCTKHGNA 337
STC HHGCT H A
Sbjct: 318 STCCHHGCTIHDTA 277
Score = 25.4 bits (49), Expect(2) = 3e-061
Identities = 9/11 (81%), Positives = 10/11 (90%)
Frame = -3 / -2
Query: 547 LNEPLSMIVLC 515
LNEPLSM+V C
Sbjct: 487 LNEPLSMMVFC 455
>gb|BQ767300.1|BQ767300 EBro08_SQ008_C18_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ008_C18 5', mRNA sequence
Length = 629
Score = 337 bits (730), Expect = 6e-092
Identities = 138/171 (80%), Positives = 152/171 (88%)
Frame = +3 / +1
Query: 177 LIRWAAEKNKERGQKIFIEDEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLY 356
LIRWAAEKN E GQ+ ED+ECSSTYCISFK NT A PPVK++R+TMRIQALRCHVLY
Sbjct: 1 LIRWAAEKNSESGQEAVTEDDECSSTYCISFKVKNTEAVPPVKDLRKTMRIQALRCHVLY 180
Query: 357 SHDGSKLNVIPVLASRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILK 536
SHDGSKLN IPVLASRSQALRYLYIRWGVELS +TV+VGE GDTDYEGLLGG+ KTIILK
Sbjct: 181 SHDGSKLNFIPVLASRSQALRYLYIRWGVELSKMTVVVGESGDTDYEGLLGGMQKTIILK 360
Query: 537 GSFNTAPNQVHANRSYSSQDVVSFDKQGIASIEGYGPDNLKSALRQFGILK 689
GSFN+ PNQ+HA RSYS +DVVSFDK GIAS+EGY PDNLKSAL+QFG L+
Sbjct: 361 GSFNSVPNQLHAARSYSLEDVVSFDKPGIASVEGYSPDNLKSALQQFGALE 513
Score = 192 bits (414), Expect = 2e-048
Identities = 96/173 (55%), Positives = 111/173 (64%)
Frame = -2 / -3
Query: 695 LVFQYTKLP*S*L*IVWTISLN*SNSLFVKGYNILG*IASVSMNLVWSSIERAFEYDSFV 516
L Q TKL *S* ++W I+L+ SNS VK YNIL IAS M LVW I+ AFEYD +
Sbjct: 519 LALQGTKLL*S*FEVIWGITLDGSNSWLVK*YNIL*RIASGGMKLVWHGIK*AFEYDGLL 340
Query: 515 HASK*SFIICVTTLTDNHGDV*QLYPPSDIQIP*SL*ARSQNRNYIQLATIMAVQNMATQ 336
HASK* FIIC+ T+NH + QLYPP IQ+P*SL + NR +QLA IMAVQ M Q
Sbjct: 339 HASK*PFIICIAAFTNNHRHLRQLYPPPYIQVP*SLGS*C*NRYEVQLAAIMAVQYMTPQ 160
Query: 335 CLYSHCPPNLLHRRCSCSIGPFK*NAVGG*AFFILNKYFLSPFFVFLSCPTN* 177
CL SHC +LHRR SI FK NAV G* F I + FLS + CPTN*
Sbjct: 159 CLNSHCLSKILHRRDGLSILHFKRNAVSG*TFVIFSNCFLSTLAILFCCPTN* 1
Score = 160 bits (343), Expect(2) = 3e-059
Identities = 70/97 (72%), Positives = 79/97 (81%)
Frame = -3 / -1
Query: 469 TITVMFDSSTPHRIYKYLKACEREAKTGITFNLLPSWLYKTWQRSACILIVLLISFTGGA 290
T TV+FDSSTPH IYKYL+A +R+A+TG+ FNLLPSWLY T R+A ILIV L SFTGG
Sbjct: 293 TTTVIFDSSTPHLIYKYLRAWDRDARTGMKFNLLPSWLYNT*HRNA*ILIVFLRSFTGGT 114
Query: 289 AAVLDPLNEMQ*VDEHSSSSINIFCPLSLFFSAAQRI 179
A+V L EMQ*VDEHSSSS+ CPLSLFFSAAQRI
Sbjct: 113 ASVFFTLKEMQ*VDEHSSSSVTASCPLSLFFSAAQRI 3
Score = 125 bits (268), Expect(2) = 2e-035
Identities = 65/119 (54%), Positives = 76/119 (63%)
Frame = +1 / +2
Query: 325 EYKHCVAMFCTAMMVAS*M*FLFWLLAHRL*GICISDGG*SCQTSP*LSVSVVTQIMKDY 504
E+KHC M+CTAMM AS* +LF +L*G CI GG*SC+ L V QIMK Y
Sbjct: 149 EFKHCGVMYCTAMMAAS*TSYLF*HHDPKL*GTCI*GGG*SCRR*RWLLVKAAIQIMKGY 328
Query: 505 LEACTKLSYSKARSILLQTKFMLTEAIHPKMLYPLTNKELLQLRDMVQTI*SQLYGNLV 681
LEAC + SYSKA I QT FM EAI +MLY LT++ELL R + Q +QLY NLV
Sbjct: 329 LEACRRPSYSKAHLIPCQTSFMPPEAIR*RMLYHLTSQELLPSRVIPQITSNQLYNNLV 505
Score = 112 bits (240), Expect(2) = 7e-042
Identities = 51/97 (52%), Positives = 66/97 (68%)
Frame = +2 / +3
Query: 179 NSLGS*EKQRKGTKNIY*G*RMLIHLLHFI*RVQYCSCTSCEGD*EDNENTSTALPCFVQ 358
NSLGS ++ R+ T++ Y* *RM IHLLHF * +Y SCEG *+DNEN+S A+ C VQ
Sbjct: 3 NSLGSRKE*REWTRSSY*R*RMFIHLLHFF*SEEY*GRPSCEGS*KDNENSSIAVSCIVQ 182
Query: 359 P*W*QVECNSCFGFSLTGFKVFVYPMGGRAVKHHRDC 469
P*W QVE ++C ++ KV VY +GGRAV+ C
Sbjct: 183 P*WQQVELHTCSSITIPSSKVLVYKVGGRAVEDDGGC 293
Score = 107 bits (228), Expect(2) = 3e-028
Identities = 59/115 (51%), Positives = 67/115 (58%)
Frame = -1 / -2
Query: 681 YQIAVELTLDCLDHIPQLKQFLVCQRIQHLGMNSFC*HELGLEQY*TSL*V**FCARLQV 502
+QI VEL L + P+ KQFL CQ IQH NSF HE GL + * SL*V* A LQV
Sbjct: 505 HQIVVELI*GYLGNNPRRKQFLACQMIQHPLANSFWRHEAGLARN*MSL*V*WSSACLQV 326
Query: 501 VLHNLCHHTHRQSR*CLTALPPIGYTNTLKPVSEKPKQELHSTCYHHGCTKHGNA 337
LHNL H+Q TALPP YT+TL+ +Q STC HHGCT H A
Sbjct: 325 ALHNLYRRFHQQPPSSSTALPPTLYTSTLELGIVMLEQV*SSTCCHHGCTIHDTA 161
Score = 89.5 bits (189), Expect(2) = 3e-059
Identities = 40/55 (72%), Positives = 42/55 (76%)
Frame = -3 / -1
Query: 679 PNCRRADFRLSGPYPSIEAIPCLSKDTTSWDE*LLLA*TWFGAVLNEPLSMIVLC 515
PNC RAD RLSG PS EAIP LS DTTS E*LL A*+WFG LNEPLSM+V C
Sbjct: 503 PNCCRADLRLSGE*PSTEAIPGLSNDTTSSSE*LLAA*SWFGTELNEPLSMMVFC 339
Score = 78.5 bits (165), Expect(2) = 7e-042
Identities = 30/55 (54%), Positives = 42/55 (76%)
Frame = +2 / +3
Query: 515 AQNYHTQRLVQYCSKPSSC*QKLFIPRCCIL*QTRNCFN*GIWSRQSKVSSTAIW 679
A+++HTQRL+ + +KP+SC QKLF CCI+*Q RNCF G++ R ++SST IW
Sbjct: 339 AEDHHTQRLI*FRAKPASCRQKLFARGCCII*QARNCFRRGLFPR*PQISSTTIW 503
Score = 43.7 bits (89), Expect(2) = 2e-035
Identities = 19/32 (59%), Positives = 23/32 (71%)
Frame = +1 / +2
Query: 178 *FVGQLRKTKKGDKKYLLRMKNAHPPTAFHLK 273
*FVGQ ++ + DKK LL+M N HP TAF LK
Sbjct: 2 *FVGQQKRIARVDKKQLLKMTNVHPLTAFLLK 97
Score = 38.2 bits (77), Expect(2) = 3e-028
Identities = 18/35 (51%), Positives = 22/35 (62%)
Frame = -1 / -2
Query: 282 YWTL*MKCSRWMSILHPQ*IFFVPFLCFSQLPNEL 178
Y +L* KCS+WM+I H Q*+ V LPNEL
Sbjct: 106 YSSL*KKCSKWMNIRHLQ*LLLVHSRYSFLLPNEL 2
>gb|CA015292.1|CA015292 HT13N20r HT Hordeum vulgare subsp. vulgare cDNA clone HT13N20
5-PRIME, mRNA sequence
Length = 544
Score = 309 bits (669), Expect = 2e-083
Identities = 126/153 (82%), Positives = 139/153 (90%)
Frame = +3 / +2
Query: 231 EDEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSKLNVIPVLASRSQ 410
ED+ECSSTYCISFK NT A PPVK++R+TMRIQALRCHVLYSHDGSKLN IPVLASRSQ
Sbjct: 17 EDDECSSTYCISFKVKNTEAVPPVKDLRKTMRIQALRCHVLYSHDGSKLNFIPVLASRSQ 196
Query: 411 ALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGSFNTAPNQVHANRSYSS 590
ALRYLYIRWGVELS +TV+VGE GDTDYEGLLGG+ KTIILKGSFN+ PNQ+HA RSYS
Sbjct: 197 ALRYLYIRWGVELSKMTVVVGESGDTDYEGLLGGMQKTIILKGSFNSVPNQLHAARSYSL 376
Query: 591 QDVVSFDKQGIASIEGYGPDNLKSALRQFGILK 689
+DVVSFDK GIAS+EGY PDNLKSAL+QFG L+
Sbjct: 377 EDVVSFDKPGIASVEGYSPDNLKSALQQFGALE 475
Score = 175 bits (377), Expect = 3e-043
Identities = 88/156 (56%), Positives = 102/156 (65%)
Frame = -2 / -1
Query: 695 LVFQYTKLP*S*L*IVWTISLN*SNSLFVKGYNILG*IASVSMNLVWSSIERAFEYDSFV 516
L Q TKL *S* *++W I+L+ SNS VK YNIL IAS M LVW I+ AFEYD +
Sbjct: 481 LALQGTKLL*S*F*VIWGITLDGSNSWLVKRYNIL*RIASGGMKLVWHGIK*AFEYDGLL 302
Query: 515 HASK*SFIICVTTLTDNHGDV*QLYPPSDIQIP*SL*ARSQNRNYIQLATIMAVQNMATQ 336
HASK* FIIC+ T+NH + QLYPP IQ+P* L + NR +QLA IMAVQ M Q
Sbjct: 301 HASK*PFIICIAAFTNNHRHLRQLYPPPYIQVP*CLGS*C*NRYEVQLAAIMAVQYMTPQ 122
Query: 335 CLYSHCPPNLLHRRCSCSIGPFK*NAVGG*AFFILN 228
CL SHC +LHRR SI FK NAV G* F I +
Sbjct: 121 CLNSHCLSKILHRRDGLSILHFKRNAVSG*TFVIFS 14
Score = 128 bits (274), Expect(2) = 1e-050
Identities = 56/83 (67%), Positives = 65/83 (78%)
Frame = -3 / -2
Query: 469 TITVMFDSSTPHRIYKYLKACEREAKTGITFNLLPSWLYKTWQRSACILIVLLISFTGGA 290
T TV+FDSSTPH IYKYL A +R+A+TG+ FNLLPSWLY T R+A I IV L SFTGG
Sbjct: 255 TTTVIFDSSTPHLIYKYLSAWDRDARTGMKFNLLPSWLYNT*HRNA*IRIVFLRSFTGGT 76
Query: 289 AAVLDPLNEMQ*VDEHSSSSINI 221
A+V L EMQ*VDEHSSSS+ +
Sbjct: 75 ASVFFTLKEMQ*VDEHSSSSVTL 7
Score = 124 bits (266), Expect(2) = 4e-030
Identities = 65/119 (54%), Positives = 76/119 (63%)
Frame = +1 / +3
Query: 325 EYKHCVAMFCTAMMVAS*M*FLFWLLAHRL*GICISDGG*SCQTSP*LSVSVVTQIMKDY 504
E+KHC M+CTAMM AS* +LF + *G CI GG*SC+ L V QIMK Y
Sbjct: 111 EFKHCGVMYCTAMMAAS*TSYLF*HHDPKH*GTCI*GGG*SCRR*RWLLVKAAIQIMKGY 290
Query: 505 LEACTKLSYSKARSILLQTKFMLTEAIHPKMLYPLTNKELLQLRDMVQTI*SQLYGNLV 681
LEAC + SYSKA I QT FM EAI +MLY LT++ELL R + Q *+QLY NLV
Sbjct: 291 LEACRRPSYSKAHLIPCQTSFMPPEAIR*RMLYRLTSQELLPSRVIPQIT*NQLYNNLV 467
Score = 97.3 bits (206), Expect(2) = 1e-037
Identities = 43/81 (53%), Positives = 53/81 (65%)
Frame = +2 / +1
Query: 227 Y*G*RMLIHLLHFI*RVQYCSCTSCEGD*EDNENTSTALPCFVQP*W*QVECNSCFGFSL 406
Y* *RM IHLLHF * +Y SCEG *+DN N+S A+ C VQP*W QVE ++C ++
Sbjct: 13 Y*R*RMFIHLLHFF*SEEY*GRPSCEGS*KDNANSSIAVSCIVQP*WQQVELHTCSSITI 192
Query: 407 TGFKVFVYPMGGRAVKHHRDC 469
KV VY +GGRAV+ C
Sbjct: 193 PSTKVLVYKVGGRAVEDDGGC 255
Score = 92.3 bits (195), Expect(2) = 1e-050
Identities = 41/55 (74%), Positives = 43/55 (78%)
Frame = -3 / -2
Query: 679 PNCRRADFRLSGPYPSIEAIPCLSKDTTSWDE*LLLA*TWFGAVLNEPLSMIVLC 515
PNC RADFRLSG PS EAIP LS DTTS E*LL A*+WFG LNEPLSM+V C
Sbjct: 465 PNCCRADFRLSGE*PSTEAIPGLSNDTTSSSE*LLAA*SWFGTELNEPLSMMVFC 301
Score = 90.0 bits (190), Expect(3) = 2e-024
Identities = 49/89 (55%), Positives = 55/89 (61%)
Frame = -1 / -3
Query: 681 YQIAVELTLDCLDHIPQLKQFLVCQRIQHLGMNSFC*HELGLEQY*TSL*V**FCARLQV 502
+QI VEL L L + P+ KQFL CQ IQH NSF HE GL + * SL*V* A LQV
Sbjct: 467 HQIVVELILGYLGNNPRRKQFLACQTIQHPLANSFWRHEAGLARN*MSL*V*WSSACLQV 288
Query: 501 VLHNLCHHTHRQSR*CLTALPPIGYTNTL 415
LHNL H+Q TALPP YT+TL
Sbjct: 287 ALHNLYRRFHQQPPSSSTALPPTLYTSTL 201
Score = 79.9 bits (168), Expect(2) = 1e-037
Identities = 31/55 (56%), Positives = 42/55 (76%)
Frame = +2 / +1
Query: 515 AQNYHTQRLVQYCSKPSSC*QKLFIPRCCIL*QTRNCFN*GIWSRQSKVSSTAIW 679
A+++HTQRL+ + +KP+SC QKLF CCI+*Q RNCF G++ R K+SST IW
Sbjct: 301 AEDHHTQRLI*FRAKPASCRQKLFARGCCIV*QARNCFRRGLFPR*PKISSTTIW 465
Score = 30.4 bits (60), Expect(3) = 2e-024
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = -1 / -3
Query: 378 STCYHHGCTKHGNA 337
STC HHGCT H A
Sbjct: 164 STCCHHGCTIHDTA 123
Score = 29.9 bits (59), Expect(3) = 2e-024
Identities = 12/19 (63%), Positives = 15/19 (78%)
Frame = -1 / -3
Query: 282 YWTL*MKCSRWMSILHPQ* 226
Y +L* KCS+WM+I H Q*
Sbjct: 68 YSSL*KKCSKWMNIRHLQ* 12
Score = 27.2 bits (53), Expect(2) = 4e-030
Identities = 11/16 (68%), Positives = 12/16 (75%)
Frame = +1 / +3
Query: 226 LLRMKNAHPPTAFHLK 273
LL+M N HP TAF LK
Sbjct: 12 LLKMTNVHPLTAFLLK 59
>gb|AF261107.1|AF261107 Hordeum vulgare sucrose-phosphate synthase mRNA, partial cds
Length = 2085
Score = 220 bits (475), Expect(3) = 2e-069
Identities = 86/156 (55%), Positives = 117/156 (75%)
Frame = +3 / +1
Query: 216 QKIFIEDEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSKLNVIPVL 395
+++ ED E SSTYC++FK N PP+KE+R+ MRIQ+LRC+ LY+H ++L+V P+
Sbjct: 1339 RQMIFEDSEHSSTYCLAFKVVNPNHLPPLKELRKLMRIQSLRCNALYNHSATRLSVTPIH 1518
Query: 396 ASRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGSFNTAPNQVHAN 575
ASRSQA+RYL++RWG+EL NI VIVGE GD+DYE LLGG+H+TIILKG FN A N++H
Sbjct: 1519 ASRSQAIRYLFVRWGIELPNIVVIVGESGDSDYEELLGGLHRTIILKGDFNIAANRIHTV 1698
Query: 576 RSYSSQDVVSFDKQGIASIEGYGPDNLKSALRQFGI 683
R Y QDVV+ D I ++G +++KSALR G+
Sbjct: 1699 RRYPLQDVVALDSSNIIEVQGCTTEDIKSALRHIGV 1806
Score = 75.3 bits (158), Expect(3) = 7e-022
Identities = 43/95 (45%), Positives = 53/95 (55%)
Frame = -3 / -2
Query: 469 TITVMFDSSTPHRIYKYLKACEREAKTGITFNLLPSWLYKTWQRSACILIVLLISFTGGA 290
T+T +F +S P R KYL A ER+A G+T +L+ WLY QRS ILI L SF GG
Sbjct: 1592 TMTTIFGNSIPQRTNKYLIA*ERDA*IGVTDSLVALWLYSALQRSDWILINFLNSFRGGR 1413
Query: 289 AAVLDPLNEMQ*VDEHSSSSINIFCPLSLFFSAAQ 185
L LN Q*V+E S SS I + F S +
Sbjct: 1412 *FGLTTLNARQ*VEECSESSKIICLSILPFLSTTE 1308
Score = 54.7 bits (113), Expect(3) = 2e-069
Identities = 19/41 (46%), Positives = 30/41 (73%)
Frame = +3 / +1
Query: 96 AELPFMIDLDYHSQIEYRWGGEGLRKTLIRWAAEKNKERGQ 218
+++ +ID ++ S IEYRWGGEGLRK L++WA + +G+
Sbjct: 1210 SKVTHVIDRNHQSHIEYRWGGEGLRKYLVKWATSVVERKGR 1332
Score = 37.3 bits (75), Expect(3) = 7e-022
Identities = 16/20 (80%), Positives = 18/20 (90%)
Frame = -3 / -2
Query: 172 FLKPSPPQRYSIWE**SRSI 113
FL+PSPPQRYSI +** RSI
Sbjct: 1286 FLRPSPPQRYSICD**FRSI 1227
Score = 29.0 bits (57), Expect(3) = 7e-022
Identities = 21/54 (38%), Positives = 24/54 (44%)
Frame = -3 / -2
Query: 679 PNCRRADFRLSGPYPSIEAIPCLSKDTTSWDE*LLLA*TWFGAVLNEPLSMIVL 518
P RRAD S P I LS TTS LL F A+L P ++VL
Sbjct: 1802 PM*RRADLMSSVVQPWTSMILELSSATTSCKGYLLTVWILFAAMLKSPFRIMVL 1641
Score = 27.2 bits (53), Expect(3) = 2e-069
Identities = 11/30 (36%), Positives = 14/30 (46%)
Frame = +3 / +1
Query: 3 GIEASXFXAFICNXXXDLXXPSSSSEDMLN 92
G+ + F AFICN + PS S N
Sbjct: 1120 GMHPAGFDAFICNSGSSIYYPSYSGNTPSN 1209
>gb|CD662445.1|CD662445 UCRHV18_01cb12_b1 Drought-stressed Dicktoo barley epidermis cDNA
library Hordeum vulgare subsp. vulgare cDNA clone
UCRHV18_01cb12, mRNA sequence
Length = 898
Score = 211 bits (456), Expect(3) = 2e-068
Identities = 84/171 (49%), Positives = 117/171 (68%)
Frame = +3 / -1
Query: 195 EKNKERGQKIFIEDEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSK 374
E+ +++ ED E SST C++F+ N PP+KE+++ MRIQ+LRCH LY+H ++
Sbjct: 637 ERRGRTEKQVIFEDAEHSSTSCLAFRVVNPNYLPPLKELQKLMRIQSLRCHALYNHSATR 458
Query: 375 LNVIPVLASRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGSFNTA 554
L+VIP+ ASRSQALRYL +RWG+EL N+ ++VGE GD+DYE L GG+HKTI+LKG FNT
Sbjct: 457 LSVIPIHASRSQALRYLSVRWGIELRNVVILVGESGDSDYEELFGGLHKTIVLKGEFNTP 278
Query: 555 PNQVHANRSYSSQDVVSFDKQGIASIEGYGPDNLKSALRQFGILKD*IFDL 707
N++H R Y QDV++ D I +EG D+L L+ GI * D+
Sbjct: 277 ANRIHTVRRYPLQDVIALDCSNIIGVEGCSTDDLTPTLKTLGIPTK*HIDI 125
Score = 60.2 bits (125), Expect(3) = 2e-068
Identities = 22/46 (47%), Positives = 32/46 (69%)
Frame = +3 / -1
Query: 81 DMLNPAELPFMIDLDYHSQIEYRWGGEGLRKTLIRWAAEKNKERGQ 218
D + + + F +D +Y S IEYRWGGEGLRK L++WA+ + RG+
Sbjct: 760 DSPSTSRVTFALDRNYQSHIEYRWGGEGLRKYLVKWASSIVERRGR 623
Score = 27.2 bits (53), Expect(3) = 2e-068
Identities = 10/21 (47%), Positives = 12/21 (57%)
Frame = +3 / -1
Query: 3 GIEASXFXAFICNXXXDLXXP 65
G+ + F AFICN DL P
Sbjct: 835 GMAPTDFDAFICNSGSDLFYP 773
>gb|BJ466481.1|BJ466481 BJ466481 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags32g04 3', mRNA sequence
Length = 688
Score = 212 bits (457), Expect(2) = 7e-066
Identities = 84/171 (49%), Positives = 117/171 (68%)
Frame = +3 / -3
Query: 195 EKNKERGQKIFIEDEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSK 374
E+ +++ ED E SST C++F+ N PP+KE+++ MRIQ+LRCH LY+H ++
Sbjct: 566 ERRGRTEKQVIFEDAEHSSTSCLAFRVVNPNYLPPLKELQKLMRIQSLRCHALYNHSATR 387
Query: 375 LNVIPVLASRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGSFNTA 554
L+VIP+ ASRSQALRYL +RWG+EL N+ ++VGE GD+DYE L GG+HKTI+LKG FNT
Sbjct: 386 LSVIPIHASRSQALRYLSVRWGIELRNVVILVGESGDSDYEELFGGLHKTIVLKGEFNTP 207
Query: 555 PNQVHANRSYSSQDVVSFDKQGIASIEGYGPDNLKSALRQFGILKD*IFDL 707
N++H R Y QDV++ D I +EG D+L L+ GI * D+
Sbjct: 206 ANRIHTVRRYPLQDVIALDCSNIIGVEGCSTDDLTPTLKMLGIPTK*HIDI 54
Score = 59.3 bits (123), Expect(2) = 7e-066
Identities = 21/41 (51%), Positives = 30/41 (73%)
Frame = +3 / -3
Query: 96 AELPFMIDLDYHSQIEYRWGGEGLRKTLIRWAAEKNKERGQ 218
+ + F +D +Y S IEYRWGGEGLRK L++WA+ + RG+
Sbjct: 674 SRVTFALDRNYQSHIEYRWGGEGLRKYLVKWASSIVERRGR 552
>gb|BJ468331.1|BJ468331 BJ468331 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags38e11 3', mRNA sequence
Length = 726
Score = 247 bits (534), Expect = 7e-065
Identities = 102/121 (84%), Positives = 111/121 (91%)
Frame = +3 / -1
Query: 327 IQALRCHVLYSHDGSKLNVIPVLASRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLL 506
IQALRCHVLYSHDGSKLN IPVLASRSQALRYLYIRWGVELS +TV+VGE GDTDYEGLL
Sbjct: 726 IQALRCHVLYSHDGSKLNFIPVLASRSQALRYLYIRWGVELSKMTVVVGESGDTDYEGLL 547
Query: 507 GGVHKTIILKGSFNTAPNQVHANRSYSSQDVVSFDKQGIASIEGYGPDNLKSALRQFGIL 686
GG+ KTIILKGSFN+ PNQ+HA RSYS +DVVSFDK GIAS+EGY PDNLKSAL+QFG L
Sbjct: 546 GGMQKTIILKGSFNSVPNQLHAARSYSLEDVVSFDKPGIASVEGYSPDNLKSALQQFGAL 367
Query: 687 K 689
+
Sbjct: 366 E 364
Score = 141 bits (302), Expect = 7e-033
Identities = 70/122 (57%), Positives = 82/122 (67%)
Frame = -2 / +1
Query: 695 LVFQYTKLP*S*L*IVWTISLN*SNSLFVKGYNILG*IASVSMNLVWSSIERAFEYDSFV 516
L Q TKL *S* *++W I+L+ SNS VK YNIL IAS M LVW I+ AFEYD +
Sbjct: 358 LALQGTKLL*S*F*VIWGITLDGSNSWLVKRYNIL*RIASGGMKLVWHGIK*AFEYDGLL 537
Query: 515 HASK*SFIICVTTLTDNHGDV*QLYPPSDIQIP*SL*ARSQNRNYIQLATIMAVQNMATQ 336
HASK* FIIC+ T+NH + QLYPP IQ+P* L + NR +QLA IMAVQ M Q
Sbjct: 538 HASK*PFIICIAAFTNNHRHLRQLYPPPYIQVP*CLGS*C*NRYEVQLAAIMAVQYMTPQ 717
Query: 335 CL 330
CL
Sbjct: 718 CL 723
Score = 122 bits (261), Expect = 3e-027
Identities = 64/118 (54%), Positives = 75/118 (63%)
Frame = +1 / -2
Query: 328 YKHCVAMFCTAMMVAS*M*FLFWLLAHRL*GICISDGG*SCQTSP*LSVSVVTQIMKDYL 507
+KHC M+CTAMM AS* +LF + *G CI GG*SC+ L V QIMK YL
Sbjct: 725 FKHCGVMYCTAMMAAS*TSYLF*HHDPKH*GTCI*GGG*SCRR*RWLLVKAAIQIMKGYL 546
Query: 508 EACTKLSYSKARSILLQTKFMLTEAIHPKMLYPLTNKELLQLRDMVQTI*SQLYGNLV 681
EAC + SYSKA I QT FM EAI +MLY LT++ELL R + Q *+QLY NLV
Sbjct: 545 EACRRPSYSKAHLIPCQTSFMPPEAIR*RMLYRLTSQELLPSRVIPQIT*NQLYNNLV 372
Score = 92.3 bits (195), Expect(2) = 1e-036
Identities = 41/55 (74%), Positives = 43/55 (78%)
Frame = -3 / +2
Query: 679 PNCRRADFRLSGPYPSIEAIPCLSKDTTSWDE*LLLA*TWFGAVLNEPLSMIVLC 515
PNC RADFRLSG PS EAIP LS DTTS E*LL A*+WFG LNEPLSM+V C
Sbjct: 374 PNCCRADFRLSGE*PSTEAIPGLSNDTTSSSE*LLAA*SWFGTELNEPLSMMVFC 538
Score = 90.0 bits (190), Expect(2) = 9e-021
Identities = 49/89 (55%), Positives = 55/89 (61%)
Frame = -1 / +3
Query: 681 YQIAVELTLDCLDHIPQLKQFLVCQRIQHLGMNSFC*HELGLEQY*TSL*V**FCARLQV 502
+QI VEL L L + P+ KQFL CQ IQH NSF HE GL + * SL*V* A LQV
Sbjct: 372 HQIVVELILGYLGNNPRRKQFLACQTIQHPLANSFWRHEAGLARN*MSL*V*WSSACLQV 551
Query: 501 VLHNLCHHTHRQSR*CLTALPPIGYTNTL 415
LHNL H+Q TALPP YT+TL
Sbjct: 552 ALHNLYRRFHQQPPSSSTALPPTLYTSTL 638
Score = 81.3 bits (171), Expect(2) = 1e-036
Identities = 32/46 (69%), Positives = 38/46 (82%)
Frame = -3 / +2
Query: 469 TITVMFDSSTPHRIYKYLKACEREAKTGITFNLLPSWLYKTWQRSA 332
T TV+FDSSTPH IYKYL A +R+A+TG+ FNLLPSWLY T R+A
Sbjct: 584 TTTVIFDSSTPHLIYKYLSAWDRDARTGMKFNLLPSWLYNT*HRNA 721
Score = 79.9 bits (168), Expect(2) = 5e-025
Identities = 31/55 (56%), Positives = 42/55 (76%)
Frame = +2 / -3
Query: 515 AQNYHTQRLVQYCSKPSSC*QKLFIPRCCIL*QTRNCFN*GIWSRQSKVSSTAIW 679
A+++HTQRL+ + +KP+SC QKLF CCI+*Q RNCF G++ R K+SST IW
Sbjct: 538 AEDHHTQRLI*FRAKPASCRQKLFARGCCIV*QARNCFRRGLFPR*PKISSTTIW 374
Score = 54.7 bits (113), Expect(2) = 5e-025
Identities = 22/47 (46%), Positives = 30/47 (63%)
Frame = +2 / -3
Query: 329 TSTALPCFVQP*W*QVECNSCFGFSLTGFKVFVYPMGGRAVKHHRDC 469
+S A+ C VQP*W QVE ++C ++ KV VY +GGRAV+ C
Sbjct: 724 SSIAVSCIVQP*WQQVELHTCSSITIPSTKVLVYKVGGRAVEDDGGC 584
Score = 30.4 bits (60), Expect(2) = 9e-021
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = -1 / +3
Query: 378 STCYHHGCTKHGNA 337
STC HHGCT H A
Sbjct: 675 STCCHHGCTIHDTA 716
>gb|BI950175.1|BI950175 HVSMEl0019E11f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0019E11f, mRNA sequence
Length = 470
Score = 245 bits (529), Expect = 3e-064
Identities = 101/122 (82%), Positives = 112/122 (91%)
Frame = +3 / +2
Query: 324 RIQALRCHVLYSHDGSKLNVIPVLASRSQALRYLYIRWGVELSNITVIVGECGDTDYEGL 503
RIQALRCHVLYSHDGSKLN IPVLASRSQALRYLYIRWGVELS +TV+VGE GDTDYEGL
Sbjct: 2 RIQALRCHVLYSHDGSKLNFIPVLASRSQALRYLYIRWGVELSKMTVVVGENGDTDYEGL 181
Query: 504 LGGVHKTIILKGSFNTAPNQVHANRSYSSQDVVSFDKQGIASIEGYGPDNLKSALRQFGI 683
LGG+ KTIILKGSFN+ PNQ+HA RSYS +DVVSFDK GIAS+EGY P+NLK+AL+QFG
Sbjct: 182 LGGMQKTIILKGSFNSVPNQLHAARSYSLEDVVSFDKPGIASVEGYSPNNLKTALQQFGA 361
Query: 684 LK 689
L+
Sbjct: 362 LE 367
Score = 139 bits (299), Expect = 2e-032
Identities = 70/124 (56%), Positives = 82/124 (66%)
Frame = -2 / -2
Query: 695 LVFQYTKLP*S*L*IVWTISLN*SNSLFVKGYNILG*IASVSMNLVWSSIERAFEYDSFV 516
L Q TKL *S *++W I+L+ SNS VK YNIL IAS M LVW I+ AFEYD +
Sbjct: 373 LALQGTKLL*SCF*VIWGITLDGSNSWLVKRYNIL*RIASGGMKLVWHGIK*AFEYDGLL 194
Query: 515 HASK*SFIICVTTLTDNHGDV*QLYPPSDIQIP*SL*ARSQNRNYIQLATIMAVQNMATQ 336
H SK* FIIC+ T+NH +*QLYPP IQ+P* L + NR +QLA IMAVQ M Q
Sbjct: 193 HPSK*PFIICIAVFTNNHRHL*QLYPPPYIQVP*CLGS*C*NRYEVQLAAIMAVQYMTPQ 14
Query: 335 CLYS 324
CL S
Sbjct: 13 CLNS 2
Score = 124 bits (265), Expect = 9e-028
Identities = 65/119 (54%), Positives = 74/119 (62%)
Frame = +1 / +3
Query: 325 EYKHCVAMFCTAMMVAS*M*FLFWLLAHRL*GICISDGG*SCQTSP*LSVSVVTQIMKDY 504
E+KHC M+CTAMM AS* +LF + *G CI GG*SCQ L V QIMK Y
Sbjct: 3 EFKHCGVMYCTAMMAAS*TSYLF*HHDPKH*GTCI*GGG*SCQR*RWLLVKTAIQIMKGY 182
Query: 505 LEACTKLSYSKARSILLQTKFMLTEAIHPKMLYPLTNKELLQLRDMVQTI*SQLYGNLV 681
LE C + SYSKA I QT FM EAI +MLY LT++ELL R + Q * QLY NLV
Sbjct: 183 LEGCRRPSYSKAHLIPCQTNFMPPEAIR*RMLYRLTSQELLPSRVIPQIT*KQLYNNLV 359
Score = 94.1 bits (199), Expect(2) = 5e-022
Identities = 50/89 (56%), Positives = 57/89 (64%)
Frame = -1 / -1
Query: 681 YQIAVELTLDCLDHIPQLKQFLVCQRIQHLGMNSFC*HELGLEQY*TSL*V**FCARLQV 502
+QI VEL L L + P+ KQFL CQ IQH NSF HE+GL + * SL*V* A LQV
Sbjct: 359 HQIVVELFLGYLGNNPRRKQFLACQTIQHPLANSFWRHEVGLARN*MSL*V*WSSASLQV 180
Query: 501 VLHNLCHHTHRQSR*CLTALPPIGYTNTL 415
LHNL H+Q LTALPP YT+TL
Sbjct: 179 ALHNLYRRFHQQPPSSLTALPPTLYTSTL 93
Score = 85.4 bits (180), Expect(2) = 4e-035
Identities = 39/55 (70%), Positives = 41/55 (74%)
Frame = -3 / -3
Query: 679 PNCRRADFRLSGPYPSIEAIPCLSKDTTSWDE*LLLA*TWFGAVLNEPLSMIVLC 515
PNC RA FRL G PS EAIP LS DTTS E*LL A*+WFG LNEPLSM+V C
Sbjct: 357 PNCCRAVFRLFGE*PSTEAIPGLSNDTTSSSE*LLAA*SWFGTELNEPLSMMVFC 193
Score = 83.1 bits (175), Expect(2) = 4e-035
Identities = 34/49 (69%), Positives = 40/49 (81%)
Frame = -3 / -3
Query: 469 TITVMFDSSTPHRIYKYLKACEREAKTGITFNLLPSWLYKTWQRSACIL 323
T TV+FDSSTPH IYKYL A +R+A+TG+ FNLLPSWLY T R+A IL
Sbjct: 147 TTTVIFDSSTPHLIYKYLSAWDRDARTGMKFNLLPSWLYNT*HRNA*IL 1
Score = 75.8 bits (159), Expect(2) = 8e-026
Identities = 30/55 (54%), Positives = 41/55 (74%)
Frame = +2 / +1
Query: 515 AQNYHTQRLVQYCSKPSSC*QKLFIPRCCIL*QTRNCFN*GIWSRQSKVSSTAIW 679
A+++HTQRL+ + +KP+SC QKLF CCI+*Q RNCF G++ + K SST IW
Sbjct: 193 AEDHHTQRLI*FRAKPTSCRQKLFARGCCIV*QARNCFRRGLFPK*PKNSSTTIW 357
Score = 61.6 bits (128), Expect(2) = 8e-026
Identities = 25/49 (51%), Positives = 32/49 (65%)
Frame = +2 / +1
Query: 323 ENTSTALPCFVQP*W*QVECNSCFGFSLTGFKVFVYPMGGRAVKHHRDC 469
EN+S A+ C VQP*W QVE ++C ++ KV VY +GGRAVK C
Sbjct: 1 ENSSIAVSCIVQP*WQQVELHTCSSITIPSTKVLVYKVGGRAVKDDGGC 147
Score = 30.4 bits (60), Expect(2) = 5e-022
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = -1 / -1
Query: 378 STCYHHGCTKHGNA 337
STC HHGCT H A
Sbjct: 56 STCCHHGCTIHDTA 15
>gb|BE601555.2|BE601555 HVSMEh0098G14f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0098G14f, mRNA sequence
Length = 636
Score = 239 bits (517), Expect = 2e-062
Identities = 98/117 (83%), Positives = 107/117 (91%)
Frame = +3 / +2
Query: 339 RCHVLYSHDGSKLNVIPVLASRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVH 518
RCHVLYSHDGSKLN IPVLASRSQALRYLYIRWGVELS +TV+VGE GDTDYEGLLGG+
Sbjct: 2 RCHVLYSHDGSKLNFIPVLASRSQALRYLYIRWGVELSKMTVVVGESGDTDYEGLLGGMQ 181
Query: 519 KTIILKGSFNTAPNQVHANRSYSSQDVVSFDKQGIASIEGYGPDNLKSALRQFGILK 689
KTIILKGSFN+ PNQ+HA RSYS +DVVSFDK GIAS+EGY PDNLKSAL+QFG L+
Sbjct: 182 KTIILKGSFNSVPNQLHAARSYSLEDVVSFDKPGIASVEGYSPDNLKSALQQFGALE 352
Score = 133 bits (285), Expect = 2e-030
Identities = 67/117 (57%), Positives = 79/117 (67%)
Frame = -2 / -3
Query: 695 LVFQYTKLP*S*L*IVWTISLN*SNSLFVKGYNILG*IASVSMNLVWSSIERAFEYDSFV 516
L Q TKL *S* *++W I+L+ SNS VK YNIL IAS M LVW I+ AFEYD +
Sbjct: 358 LALQGTKLL*S*F*VIWGITLDGSNSWLVKRYNIL*RIASGGMKLVWHGIK*AFEYDGLL 179
Query: 515 HASK*SFIICVTTLTDNHGDV*QLYPPSDIQIP*SL*ARSQNRNYIQLATIMAVQNM 345
H SK* FIIC+ T+NH +*QLYPP IQ+P* L + NR +QLA IMAVQ M
Sbjct: 178 HPSK*PFIICIAAFTNNHRHL*QLYPPPYIQVP*CLGS*C*NRYEVQLAAIMAVQYM 8
Score = 112 bits (239), Expect = 3e-024
Identities = 61/112 (54%), Positives = 70/112 (62%)
Frame = +1 / +3
Query: 346 MFCTAMMVAS*M*FLFWLLAHRL*GICISDGG*SCQTSP*LSVSVVTQIMKDYLEACTKL 525
M+CTAMM AS* +LF + *G CI GG*SCQ L V QIMK YLE C +
Sbjct: 9 MYCTAMMAAS*TSYLF*HHDPKH*GTCI*GGG*SCQR*RWLLVKAAIQIMKGYLEGCRRP 188
Query: 526 SYSKARSILLQTKFMLTEAIHPKMLYPLTNKELLQLRDMVQTI*SQLYGNLV 681
SYSKA I QT FM EAI +MLY LT++ELL R + Q *+QLY NLV
Sbjct: 189 SYSKAHLIPCQTSFMPPEAIR*RMLYRLTSQELLPSRVIPQIT*NQLYNNLV 344
Score = 93.6 bits (198), Expect(2) = 2e-021
Identities = 50/89 (56%), Positives = 56/89 (62%)
Frame = -1 / -2
Query: 681 YQIAVELTLDCLDHIPQLKQFLVCQRIQHLGMNSFC*HELGLEQY*TSL*V**FCARLQV 502
+QI VEL L L + P+ KQFL CQ IQH NSF HE GL + * SL*V* A LQV
Sbjct: 344 HQIVVELILGYLGNNPRRKQFLACQTIQHPLANSFWRHEAGLARN*MSL*V*WSSASLQV 165
Query: 501 VLHNLCHHTHRQSR*CLTALPPIGYTNTL 415
LHNL H+Q LTALPP YT+TL
Sbjct: 164 ALHNLYRRFHQQPPSSLTALPPTLYTSTL 78
Score = 92.3 bits (195), Expect(2) = 7e-036
Identities = 41/55 (74%), Positives = 43/55 (78%)
Frame = -3 / -1
Query: 679 PNCRRADFRLSGPYPSIEAIPCLSKDTTSWDE*LLLA*TWFGAVLNEPLSMIVLC 515
PNC RADFRLSG PS EAIP LS DTTS E*LL A*+WFG LNEPLSM+V C
Sbjct: 342 PNCCRADFRLSGE*PSTEAIPGLSNDTTSSSE*LLAA*SWFGTELNEPLSMMVFC 178
Score = 79.9 bits (168), Expect(2) = 5e-025
Identities = 31/55 (56%), Positives = 42/55 (76%)
Frame = +2 / +1
Query: 515 AQNYHTQRLVQYCSKPSSC*QKLFIPRCCIL*QTRNCFN*GIWSRQSKVSSTAIW 679
A+++HTQRL+ + +KP+SC QKLF CCI+*Q RNCF G++ R K+SST IW
Sbjct: 178 AEDHHTQRLI*FRAKPASCRQKLFARGCCIV*QARNCFRRGLFPR*PKISSTTIW 342
Score = 79.0 bits (166), Expect(2) = 7e-036
Identities = 31/44 (70%), Positives = 36/44 (81%)
Frame = -3 / -1
Query: 469 TITVMFDSSTPHRIYKYLKACEREAKTGITFNLLPSWLYKTWQR 338
T TV+FDSSTPH IYKYL A +R+A+TG+ FNLLPSWLY T R
Sbjct: 132 TTTVIFDSSTPHLIYKYLSAWDRDARTGMKFNLLPSWLYNT*HR 1
Score = 54.7 bits (113), Expect(2) = 5e-025
Identities = 22/44 (50%), Positives = 28/44 (63%)
Frame = +2 / +1
Query: 338 ALPCFVQP*W*QVECNSCFGFSLTGFKVFVYPMGGRAVKHHRDC 469
A+ C VQP*W QVE ++C ++ KV VY +GGRAVK C
Sbjct: 1 AVSCIVQP*WQQVELHTCSSITIPSTKVLVYKVGGRAVKDDGGC 132
Score = 29.0 bits (57), Expect(2) = 2e-021
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = -1 / -2
Query: 378 STCYHHGCTKH 346
STC HHGCT H
Sbjct: 41 STCCHHGCTIH 9
>gb|BE437516.1|BE437516 SFR004.E11F990621 ITEC SFR Barley Leaf Epidermis Library Hordeum
vulgare subsp. vulgare cDNA clone SF, mRNA sequence
Length = 804
Score = 186 bits (400), Expect(4) = 4e-062
Identities = 72/123 (58%), Positives = 96/123 (78%)
Frame = +3 / +2
Query: 216 QKIFIEDEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSKLNVIPVL 395
+++ ED E SSTYC++FK N PP+KE+R+ MRIQ+LRC+ LY+H ++L+V P+
Sbjct: 335 RQMIFEDSEHSSTYCLAFKVVNPNHLPPLKELRKLMRIQSLRCNALYNHSATRLSVTPIH 514
Query: 396 ASRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGSFNTAPNQVHAN 575
ASRSQA+RYL++RWG+EL NI VIVGE GD+DYE LLGG+H+TI LKG FN A N++H
Sbjct: 515 ASRSQAIRYLFVRWGIELPNIVVIVGESGDSDYEELLGGLHRTINLKGDFNIAANRIHTV 694
Query: 576 RSY 584
R Y
Sbjct: 695 RRY 703
Score = 54.7 bits (113), Expect(4) = 4e-062
Identities = 19/41 (46%), Positives = 30/41 (73%)
Frame = +3 / +2
Query: 96 AELPFMIDLDYHSQIEYRWGGEGLRKTLIRWAAEKNKERGQ 218
+++ +ID ++ S IEYRWGGEGLRK L++WA + +G+
Sbjct: 206 SKVTHVIDRNHQSHIEYRWGGEGLRKYLVKWATSVVERKGR 328
Score = 27.6 bits (54), Expect(4) = 4e-062
Identities = 11/29 (37%), Positives = 18/29 (62%)
Frame = +3 / +1
Query: 591 QDVVSFDKQGIASIEGYGPDNLKSALRQF 677
QDVV+ D I ++ +++KSALR +
Sbjct: 709 QDVVALDSVNIIEVQXCTXEDIKSALRTY 795
Score = 27.2 bits (53), Expect(4) = 4e-062
Identities = 11/30 (36%), Positives = 14/30 (46%)
Frame = +3 / +2
Query: 3 GIEASXFXAFICNXXXDLXXPSSSSEDMLN 92
G+ + F AFICN + PS S N
Sbjct: 116 GMHPAGFDAFICNSGSSIYYPSYSGNTPSN 205
>gb|BU984860.1|BU984860 HF05E11r HF Hordeum vulgare subsp. vulgare cDNA clone HF05E11
5-PRIME, mRNA sequence
Length = 584
Score = 195 bits (420), Expect(2) = 5e-061
Identities = 74/139 (53%), Positives = 103/139 (74%)
Frame = +3 / +1
Query: 195 EKNKERGQKIFIEDEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSK 374
E+ +++ ED E SST C++F+ N PP+KE+++ MRIQ+LRCH LY+H ++
Sbjct: 163 ERRGRTEKQVIFEDAEHSSTSCLAFRVVNPNYLPPLKELQKLMRIQSLRCHALYNHSATR 342
Query: 375 LNVIPVLASRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGSFNTA 554
L+VIP+ ASRSQALRYL +RWG+EL N+ ++VGE GD+DYE L GG+HKTI+LKG FNT
Sbjct: 343 LSVIPIHASRSQALRYLSVRWGIELRNVVILVGESGDSDYEELFGGLHKTIVLKGEFNTP 522
Query: 555 PNQVHANRSYSSQDVVSFD 611
N++H R Y QDV++ D
Sbjct: 523 ANRIHTVRRYPLQDVIALD 579
Score = 60.2 bits (125), Expect(2) = 5e-061
Identities = 22/46 (47%), Positives = 32/46 (69%)
Frame = +3 / +1
Query: 81 DMLNPAELPFMIDLDYHSQIEYRWGGEGLRKTLIRWAAEKNKERGQ 218
D + + + F +D +Y S IEYRWGGEGLRK L++WA+ + RG+
Sbjct: 40 DSPSTSRVTFALDRNYQSHIEYRWGGEGLRKYLVKWASSIVERRGR 177
>gb|AV917170.1|AV917170 AV917170 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags8c04 5', mRNA sequence
Length = 630
Score = 219 bits (473), Expect = 2e-056
Identities = 86/154 (55%), Positives = 115/154 (74%)
Frame = +3 / +2
Query: 222 IFIEDEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSKLNVIPVLAS 401
+ ED E SSTYC++FK N PP+KE+R+ MRIQ+LRC+ LY+H ++L+V P+ AS
Sbjct: 59 MIFEDSEHSSTYCLAFKVVNPNHLPPLKELRKLMRIQSLRCNALYNHSATRLSVTPIHAS 238
Query: 402 RSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGSFNTAPNQVHANRS 581
RSQA+RYL++RWG+EL NI VIVGE GD+DYE LLGG+H+TIILKG FN A N++H R
Sbjct: 239 RSQAIRYLFVRWGIELPNIVVIVGESGDSDYEELLGGLHRTIILKGDFNIAANRIHTVRR 418
Query: 582 YSSQDVVSFDKQGIASIEGYGPDNLKSALRQFGI 683
Y QDVV+ D I ++G +++KSALR G+
Sbjct: 419 YPLQDVVALDSSNIIEVQGCTTEDIKSALRHIGV 520
>gb|CA022248.1|CA022248 HZ42J07r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ42J07
5-PRIME, mRNA sequence
Length = 594
Score = 211 bits (456), Expect = 4e-054
Identities = 84/171 (49%), Positives = 117/171 (68%)
Frame = +3 / +3
Query: 195 EKNKERGQKIFIEDEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSK 374
E+ +++ ED E SST C++F+ N PP+KE+++ MRIQ+LRCH LY+H ++
Sbjct: 45 ERRGRTEKQVIFEDAEHSSTSCLAFRVVNPNYLPPLKELQKLMRIQSLRCHALYNHSATR 224
Query: 375 LNVIPVLASRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGSFNTA 554
L+VIP+ ASRSQALRYL +RWG+EL N+ ++VGE GD+DYE L GG+HKTI+LKG FNT
Sbjct: 225 LSVIPIHASRSQALRYLSVRWGIELRNVVILVGESGDSDYEELFGGLHKTIVLKGEFNTP 404
Query: 555 PNQVHANRSYSSQDVVSFDKQGIASIEGYGPDNLKSALRQFGILKD*IFDL 707
N++H R Y QDV++ D I +EG D+L L+ GI * D+
Sbjct: 405 ANRIHTVRRYPLQDVIALDCSNIIGVEGCSTDDLTPTLKTLGIPTK*HIDI 557
>gb|AV931912.1|AV931912 AV931912 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd26l03 3', mRNA sequence
Length = 653
Score = 177 bits (382), Expect(2) = 4e-054
Identities = 69/113 (61%), Positives = 91/113 (80%)
Frame = +3 / -1
Query: 216 QKIFIEDEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSKLNVIPVL 395
+++ ED E SSTYC++FK N PP+KE+R+ MRIQ+LRC+ LY+H ++L+V P+
Sbjct: 527 RQMIFEDSEHSSTYCLAFKVVNPNHLPPLKELRKLMRIQSLRCNALYNHSATRLSVTPIH 348
Query: 396 ASRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGSFNTA 554
ASRSQA+RYL++RWG+EL NI VIVGE GD+DYE LLGG+H+TIILKG FN A
Sbjct: 347 ASRSQAIRYLFVRWGIELPNIVVIVGESGDSDYEELLGGLHRTIILKGDFNIA 189
Score = 54.2 bits (112), Expect(2) = 4e-054
Identities = 19/36 (52%), Positives = 27/36 (75%)
Frame = +3 / -1
Query: 111 MIDLDYHSQIEYRWGGEGLRKTLIRWAAEKNKERGQ 218
+ID ++ S IEYRWGGEGLRK L++WA + +G+
Sbjct: 641 VIDRNHQSHIEYRWGGEGLRKYLVKWATSVVERKGR 534
>gb|AV914348.1|AV914348 AV914348 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags5m14 5', mRNA sequence
Length = 475
Score = 199 bits (430), Expect(2) = 3e-053
Identities = 78/132 (59%), Positives = 103/132 (78%)
Frame = +3 / +1
Query: 216 QKIFIEDEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSKLNVIPVL 395
+++ ED E SSTYC++FK N PP+KE+R+ MRIQ+LRC+ LY+H ++L+V P+
Sbjct: 79 RQMIFEDSEHSSTYCLAFKVVNPNHLPPLKELRKLMRIQSLRCNALYNHSATRLSVTPIH 258
Query: 396 ASRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGSFNTAPNQVHAN 575
ASRSQA+RYL++RWG+EL NI VIVGE GD+DYE LLGG+H+TIILKG FN A N++H
Sbjct: 259 ASRSQAIRYLFVRWGIELPNIVVIVGESGDSDYEELLGGLHRTIILKGDFNIAANRIHTV 438
Query: 576 RSYSSQDVVSFD 611
R Y QDVV+ D
Sbjct: 439 RRYPLQDVVALD 474
Score = 29.5 bits (58), Expect(2) = 3e-053
Identities = 10/21 (47%), Positives = 15/21 (71%)
Frame = +3 / +1
Query: 156 GEGLRKTLIRWAAEKNKERGQ 218
GEGLRK L++WA + +G+
Sbjct: 10 GEGLRKYLVKWATSVVERKGR 72
>gb|BG418110.1|BG418110 HVSMEk0021F18f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0021F18f, mRNA sequence
Length = 668
Score = 193 bits (417), Expect = 1e-048
Identities = 82/113 (72%), Positives = 93/113 (82%)
Frame = +3 / +1
Query: 348 VLYSHDGSKLNVIPVLASRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTI 527
VLYSHDG K N IPVL+S SQALRYLYI WGVELSN+TV+VGE D+ YE LLGG+ KTI
Sbjct: 1 VLYSHDGCKSNFIPVLSSLSQALRYLYIMWGVELSNMTVVVGESCDSHYERLLGGMQKTI 180
Query: 528 ILKGSFNTAPNQVHANRSYSSQDVVSFDKQGIASIEGYGPDNLKSALRQFGIL 686
ILKGSFN+ PNQ+HA SYS +DVVSFDK IA +EGY P+NLKS L+QFG L
Sbjct: 181 ILKGSFNSVPNQLHATTSYSLKDVVSFDKPRIAYVEGYSPNNLKSTLQQFGAL 339
Score = 108 bits (230), Expect = 6e-023
Identities = 58/111 (52%), Positives = 68/111 (61%)
Frame = +1 / +2
Query: 349 FCTAMMVAS*M*FLFWLLAHRL*GICISDGG*SCQTSP*LSVSVVTQIMKDYLEACTKLS 528
+CTAMM AS +LF+ + *G CI GG*SCQT *L V IM YLE C + S
Sbjct: 2 YCTAMMAASRTSYLFYHHYPKH*GTCI*CGG*SCQT*Q*LLVKAAIHIMNGYLEGCRRPS 181
Query: 529 YSKARSILLQTKFMLTEAIHPKMLYPLTNKELLQLRDMVQTI*SQLYGNLV 681
YSKA I QT FM +AI +MLY LT+ ELL R + Q *+QLY NLV
Sbjct: 182 YSKAHLIPCQTSFMPPQAIR*RMLYRLTSHELLTSRVIPQIT*NQLYNNLV 334
Score = 105 bits (224), Expect(2) = 3e-024
Identities = 53/96 (55%), Positives = 64/96 (66%)
Frame = -2 / -3
Query: 695 LVFQYTKLP*S*L*IVWTISLN*SNSLFVKGYNILG*IASVSMNLVWSSIERAFEYDSFV 516
L Q TKL *S* *++W I+L+ SNS VK YNIL IA M LVW I+ AFEYD +
Sbjct: 348 LALQGTKLL*S*F*VIWGITLDVSNSWLVKRYNIL*RIACGGMKLVWHGIK*AFEYDGLL 169
Query: 515 HASK*SFIICVTTLTDNHGDV*QLYPPSDIQIP*SL 408
H SK* FI+ + T+N+ V*QLYPP IQ+P* L
Sbjct: 168 HPSK*PFIM*IAAFTNNYCHV*QLYPPHYIQVP*CL 61
Score = 81.7 bits (172), Expect(2) = 3e-029
Identities = 36/58 (62%), Positives = 42/58 (72%)
Frame = -3 / -1
Query: 688 FNIPNCRRADFRLSGPYPSIEAIPCLSKDTTSWDE*LLLA*TWFGAVLNEPLSMIVLC 515
+ PNC R DFRL G PS AI LS DTTS+ E*L++A*+WFG LNEPLSM+V C
Sbjct: 341 YRAPNCCRVDFRLFGE*PST*AIRGLSNDTTSFSE*LVVA*SWFGTELNEPLSMMVFC 168
Score = 69.8 bits (146), Expect(2) = 5e-021
Identities = 26/55 (47%), Positives = 39/55 (70%)
Frame = +2 / +3
Query: 515 AQNYHTQRLVQYCSKPSSC*QKLFIPRCCIL*QTRNCFN*GIWSRQSKVSSTAIW 679
A+++HTQRL+ + +KP+SC KLF CCI+*Q NC G++ + K++ST IW
Sbjct: 168 AEDHHTQRLI*FRAKPASCHHKLFAKGCCIV*QATNCLRRGLFPK*PKINSTTIW 332
Score = 67.0 bits (140), Expect(2) = 3e-029
Identities = 26/39 (66%), Positives = 31/39 (79%)
Frame = -3 / -1
Query: 469 TITVMFDSSTPHRIYKYLKACEREAKTGITFNLLPSWLY 353
T TVMFDSSTPH IYKYL A + + +TG+ F+L PSWLY
Sbjct: 122 TTTVMFDSSTPHIIYKYLSAWDSDDRTGMKFDLQPSWLY 6
Score = 51.5 bits (106), Expect(2) = 5e-021
Identities = 21/39 (53%), Positives = 26/39 (66%)
Frame = +2 / +3
Query: 353 VQP*W*QVECNSCFGFSLTGFKVFVYPMGGRAVKHHRDC 469
VQP*W QVE ++C ++ KV VY +GGRAVKH C
Sbjct: 6 VQP*WLQVELHTCSIITIPSTKVLVYNVGGRAVKHDSSC 122
Score = 26.7 bits (52), Expect(2) = 3e-024
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = -1 / -2
Query: 378 STCYHHGCT 352
STC HHGCT
Sbjct: 31 STCSHHGCT 5
>gb|CA011510.1|CA011510 HT05E02u HT Hordeum vulgare subsp. vulgare cDNA clone HT05E02
3-PRIME, mRNA sequence
Length = 493
Score = 184 bits (396), Expect = 8e-046
Identities = 72/135 (53%), Positives = 97/135 (71%)
Frame = +3 / -1
Query: 303 KEIRRTMRIQALRCHVLYSHDGSKLNVIPVLASRSQALRYLYIRWGVELSNITVIVGECG 482
KE+++ MRIQ+LRCH LY+H ++L+VIP+ ASRSQALRYL +RWG+EL N+ ++VGE G
Sbjct: 493 KELQKLMRIQSLRCHALYNHSATRLSVIPIHASRSQALRYLSVRWGIELRNVVILVGESG 314
Query: 483 DTDYEGLLGGVHKTIILKGSFNTAPNQVHANRSYSSQDVVSFDKQGIASIEGYGPDNLKS 662
D+DYE L GG+HKTI+LKG FNT N++H R Y QDV++ D I +EG D+L
Sbjct: 313 DSDYEELFGGLHKTIVLKGEFNTPANRIHTVRRYPLQDVIALDCSNIIGVEGCSTDDLTP 134
Query: 663 ALRQFGILKD*IFDL 707
L+ GI * D+
Sbjct: 133 TLKTLGIPTK*HIDI 89
>gb|BQ768491.1|BQ768491 EBro08_SQ011_I24_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ011_I24 5', mRNA sequence
Length = 632
Score = 152 bits (327), Expect = 3e-036
Identities = 62/80 (77%), Positives = 71/80 (88%)
Frame = +3 / +2
Query: 450 SNITVIVGECGDTDYEGLLGGVHKTIILKGSFNTAPNQVHANRSYSSQDVVSFDKQGIAS 629
S +TV+VGE GDTDYEGLLGG+ KTIILKGSFN+ PNQ+HA RSYS +DVVSFDK GIAS
Sbjct: 2 SKMTVVVGESGDTDYEGLLGGMQKTIILKGSFNSVPNQLHAARSYSLEDVVSFDKPGIAS 181
Query: 630 IEGYGPDNLKSALRQFGILK 689
+EGY PDNLKSAL+QFG L+
Sbjct: 182 VEGYSPDNLKSALQQFGALE 241
>gb|AV834625.1|AV834625 AV834625 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags18o22, mRNA sequence
Length = 569
Score = 150 bits (322), Expect = 1e-035
Identities = 61/77 (79%), Positives = 69/77 (89%)
Frame = +3 / -2
Query: 459 TVIVGECGDTDYEGLLGGVHKTIILKGSFNTAPNQVHANRSYSSQDVVSFDKQGIASIEG 638
TV+VGE GDTDYEGLLGG+ KTIILKGSFN+ PNQ+HA RSYS +DVVSFDK GIAS+EG
Sbjct: 568 TVVVGESGDTDYEGLLGGMQKTIILKGSFNSVPNQLHAARSYSLEDVVSFDKPGIASVEG 389
Query: 639 YGPDNLKSALRQFGILK 689
Y PDNLKSAL+QFG L+
Sbjct: 388 YSPDNLKSALQQFGALE 338
>gb|BM444184.1|BM444184 EBem09_SQ004_G12_R embryo, 1 Day germination, no treatment, cv
Optic, EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ004_G12 5', mRNA sequence
Length = 406
Score = 145 bits (311), Expect = 4e-034
Identities = 57/96 (59%), Positives = 73/96 (76%)
Frame = +3 / +1
Query: 396 ASRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGSFNTAPNQVHAN 575
ASRSQA+RYL++RWG+EL NI VIVGE GD+DYE LLGG+H+TIILKG FN A N++H
Sbjct: 1 ASRSQAIRYLFVRWGIELPNIVVIVGESGDSDYEELLGGLHRTIILKGDFNIAANRIHTV 180
Query: 576 RSYSSQDVVSFDKQGIASIEGYGPDNLKSALRQFGI 683
R Y QDVV+ D I ++G +++KSALR G+
Sbjct: 181 RRYPLQDVVALDSSNIIEVQGCTTEDIKSALRHIGV 288
>gb|BU989662.1|BU989662 HF22F18r HF Hordeum vulgare subsp. vulgare cDNA clone HF22F18
5-PRIME, mRNA sequence
Length = 484
Score = 135 bits (290), Expect = 3e-031
Identities = 55/69 (79%), Positives = 62/69 (89%)
Frame = +3 / +1
Query: 483 DTDYEGLLGGVHKTIILKGSFNTAPNQVHANRSYSSQDVVSFDKQGIASIEGYGPDNLKS 662
DTDYEGLLGG+ KTIILKGSFN+ PNQ+HA RSYS +DVVSFDK GIAS+EGY PDNLKS
Sbjct: 1 DTDYEGLLGGMQKTIILKGSFNSVPNQLHAARSYSLEDVVSFDKPGIASVEGYSPDNLKS 180
Query: 663 ALRQFGILK 689
AL+QFG L+
Sbjct: 181 ALQQFGALE 207
>gb|BU990273.1|BU990273 HF24J09r HF Hordeum vulgare subsp. vulgare cDNA clone HF24J09
5-PRIME, mRNA sequence
Length = 480
Score = 135 bits (290), Expect = 3e-031
Identities = 55/69 (79%), Positives = 62/69 (89%)
Frame = +3 / +1
Query: 483 DTDYEGLLGGVHKTIILKGSFNTAPNQVHANRSYSSQDVVSFDKQGIASIEGYGPDNLKS 662
DTDYEGLLGG+ KTIILKGSFN+ PNQ+HA RSYS +DVVSFDK GIAS+EGY PDNLKS
Sbjct: 1 DTDYEGLLGGMQKTIILKGSFNSVPNQLHAARSYSLEDVVSFDKPGIASVEGYSPDNLKS 180
Query: 663 ALRQFGILK 689
AL+QFG L+
Sbjct: 181 ALQQFGALE 207
>gb|CB859330.1|CB859330 HI10C07w HI Hordeum vulgare subsp. vulgare cDNA clone HI10C07
3-PRIME, mRNA sequence
Length = 590
Score = 135 bits (290), Expect = 3e-031
Identities = 55/69 (79%), Positives = 62/69 (89%)
Frame = +3 / -1
Query: 483 DTDYEGLLGGVHKTIILKGSFNTAPNQVHANRSYSSQDVVSFDKQGIASIEGYGPDNLKS 662
DTDYEGLLGG+ KTIILKGSFN+ PNQ+HA RSYS +DVVSFDK GIAS+EGY PDNLKS
Sbjct: 590 DTDYEGLLGGMQKTIILKGSFNSVPNQLHAARSYSLEDVVSFDKPGIASVEGYSPDNLKS 411
Query: 663 ALRQFGILK 689
AL+QFG L+
Sbjct: 410 ALQQFGALE 384
>gb|BU994139.1|BU994139 HM05O02r HM Hordeum vulgare subsp. vulgare cDNA clone HM05O02
5-PRIME, mRNA sequence
Length = 593
Score = 126 bits (270), Expect = 2e-028
Identities = 46/102 (45%), Positives = 74/102 (72%)
Frame = +3 / +2
Query: 234 DEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSKLNVIPVLASRSQA 413
D E + +C+SF + + E+R +R++ LRCH++Y + +++ V+P++ASRSQA
Sbjct: 266 DVESCNAHCVSFFVRDPKKVRTIDEMRERLRMRGLRCHLMYCRNSTRMQVVPLMASRSQA 445
Query: 414 LRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKG 539
LRYL++RWG+ + N+ +IVGE GDTD E +L G+HKT+I+KG
Sbjct: 446 LRYLFVRWGLAVGNMYLIVGEHGDTDREEMLSGLHKTVIVKG 571
>gb|BM442430.2|BM442430 EBan01_SQ003_C15_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_C15 5', mRNA sequence
Length = 504
Score = 125 bits (267), Expect = 5e-028
Identities = 45/102 (44%), Positives = 74/102 (72%)
Frame = +3 / +1
Query: 234 DEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSKLNVIPVLASRSQA 413
D E + +C+SF + + E+R +R++ LRCH++Y + +++ V+P++ASRSQA
Sbjct: 67 DVESCNAHCVSFFVRDPKKVRTIDEMRERLRMRGLRCHLMYCRNSTRMQVVPLMASRSQA 246
Query: 414 LRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKG 539
L+YL++RWG+ + N+ +IVGE GDTD E +L G+HKT+I+KG
Sbjct: 247 LKYLFVRWGLAVGNMYLIVGEHGDTDREEMLSGLHKTVIVKG 372
>gb|CA016632.1|CA016632 HV08B18u HV Hordeum vulgare subsp. vulgare cDNA clone HV08B18
3-PRIME, mRNA sequence
Length = 559
Score = 117 bits (251), Expect = 8e-026
Identities = 46/95 (48%), Positives = 62/95 (65%)
Frame = +3 / -2
Query: 423 LYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGSFNTAPNQVHANRSYSSQDVV 602
L +RWG+EL N+ ++VGE GD+DYE L GG+HKTI+LKG FNT N++H R Y QDV+
Sbjct: 558 LSVRWGIELRNVVILVGESGDSDYEELFGGLHKTIVLKGEFNTPANRIHTVRRYPLQDVI 379
Query: 603 SFDKQGIASIEGYGPDNLKSALRQFGILKD*IFDL 707
+ D I +EG D+L L+ GI * D+
Sbjct: 378 ALDCSNIIGVEGCSTDDLTPTLKTLGIPTK*HIDI 274
>gb|BJ454449.1|BJ454449 BJ454449 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak24h18 5', mRNA sequence
Length = 405
Score = 117 bits (250), Expect = 1e-025
Identities = 46/111 (41%), Positives = 74/111 (66%)
Frame = +3 / +3
Query: 234 DEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSKLNVIPVLASRSQA 413
D S +C ++ ++ + V IR+ +R++ RC+++Y+ ++LNVIP+ ASR +A
Sbjct: 72 DASACSVHCHAYAATDASKVRKVDSIRQQLRMRGFRCNLVYTRACTRLNVIPLSASRPRA 251
Query: 414 LRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGSFNTAPNQV 566
LRYL I+WG++LS + V+VGE GDTD E LL G+H+T+IL G + Q+
Sbjct: 252 LRYLSIQWGIDLSKVAVLVGEAGDTDREKLLPGLHRTLILPGMVSRGSEQL 404
>gb|CB874869.1|CB874869 HX06J01w HX Hordeum vulgare subsp. vulgare cDNA clone HX06J01
3-PRIME, mRNA sequence
Length = 563
Score = 115 bits (245), Expect = 5e-025
Identities = 46/82 (56%), Positives = 59/82 (71%)
Frame = +3 / -2
Query: 438 GVELSNITVIVGECGDTDYEGLLGGVHKTIILKGSFNTAPNQVHANRSYSSQDVVSFDKQ 617
G+EL NI VIVGE GD+DYE LLGG+H+TIILKG FN A N++H R Y QDVV+ D
Sbjct: 562 GIELPNIVVIVGESGDSDYEELLGGLHRTIILKGDFNIAANRIHTVRRYPLQDVVALDSS 383
Query: 618 GIASIEGYGPDNLKSALRQFGI 683
I ++G +++KSALR G+
Sbjct: 382 NIIEVQGCTTEDIKSALRHIGV 317
>gb|CB880803.1|CB880803 HM07O02w HM Hordeum vulgare subsp. vulgare cDNA clone HM07O02
3-PRIME, mRNA sequence
Length = 626
Score = 111 bits (238), Expect = 5e-024
Identities = 39/73 (53%), Positives = 61/73 (83%)
Frame = +3 / -1
Query: 321 MRIQALRCHVLYSHDGSKLNVIPVLASRSQALRYLYIRWGVELSNITVIVGECGDTDYEG 500
+R++ LRCH++Y + +++ V+P++ASRSQALRYL++RWG+ + N+ +IVGE GDTD E
Sbjct: 623 LRMRGLRCHLMYCRNSTRMQVVPLMASRSQALRYLFVRWGLAVGNMYLIVGEHGDTDREE 444
Query: 501 LLGGVHKTIILKG 539
+L G+HKT+I+KG
Sbjct: 443 MLSGLHKTVIVKG 405
>gb|AL503268.1|AL503268 AL503268 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW01G06T 5', mRNA sequence
Length = 683
Score = 54.7 bits (113), Expect(4) = 2e-023
Identities = 19/41 (46%), Positives = 30/41 (73%)
Frame = +3 / +1
Query: 96 AELPFMIDLDYHSQIEYRWGGEGLRKTLIRWAAEKNKERGQ 218
+++ +ID ++ S IEYRWGGEGLRK L++WA + +G+
Sbjct: 364 SKVTHVIDRNHQSHIEYRWGGEGLRKYLVKWATSVVERKGR 486
Score = 46.4 bits (95), Expect(4) = 2e-023
Identities = 19/39 (48%), Positives = 27/39 (69%)
Frame = +3 / +1
Query: 216 QKIFIEDEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQ 332
+++ ED E SSTY ++FK N PP+KE+R+ MRIQ
Sbjct: 493 RQMIFEDSEHSSTYXLAFKVVNPNHLPPLKELRKLMRIQ 609
Score = 35.9 bits (72), Expect(4) = 2e-023
Identities = 12/25 (48%), Positives = 20/25 (80%)
Frame = +3 / +2
Query: 330 QALRCHVLYSHDGSKLNVIPVLASR 404
++LRC+ LY+H ++L+V P+ ASR
Sbjct: 608 RSLRCNALYNHSATRLSVTPIHASR 682
Score = 26.7 bits (52), Expect(4) = 2e-023
Identities = 12/30 (40%), Positives = 15/30 (50%)
Frame = +3 / +1
Query: 3 GIEASXFXAFICNXXXDLXXPSSSSEDMLN 92
G+ + F AFICN + PS S N
Sbjct: 274 GMHPAGFDAFICNXGSSIYYPSYSGNTPSN 363
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 407,596,558
Number of Sequences: 312970
Number of extensions: 5759819
Number of successful extensions: 26794
Number of sequences better than 1.0e-020: 37
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 11873254398
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)