TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3092809.2.1
(652 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AL506124.1|AL506124 AL506124 Hordeum vulgare Barke devel... 60 1e-020
>gb|AL506124.1|AL506124 AL506124 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY02B21T 5',
mRNA sequence
Length = 646
Score = 60.2 bits (125), Expect(2) = 1e-020
Identities = 25/48 (52%), Positives = 33/48 (68%)
Frame = +2 / +1
Query: 53 PHVYTASKHAVVGLTKSVAAELGRHGVRVNCVSPYAVPTALSMPHLPQ 196
PH Y+ SK AV+G+ ++VA E+ R GVRVN +SP +PT L M L Q
Sbjct: 157 PHPYSVSKAAVLGVVRAVAGEMARSGVRVNAISPNYIPTPLVMRILEQ 300
Score = 59.3 bits (123), Expect(2) = 1e-020
Identities = 23/38 (60%), Positives = 31/38 (81%)
Frame = +2 / +1
Query: 257 LKGVDAMPEDVAQAVLYLASDEARYVSAVNLMVDGGFT 370
++G P+D+A+A LYLASDEA+YV+ NL+VDGGFT
Sbjct: 361 MEGAVLEPDDIARAALYLASDEAKYVNGHNLVVDGGFT 474
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 261,356,717
Number of Sequences: 312970
Number of extensions: 3887310
Number of successful extensions: 128712
Number of sequences better than 1.0e-020: 2
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6998756985
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)