TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3089980.2.1
(540 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV927308.1|AV927308 AV927308 K. Sato unpublished cDNA li... 151 3e-036
gb|BM817239.1|BM817239 HC05A08_T3.ab1 HC Hordeum vulgare su... 119 5e-034
gb|BU987748.1|BU987748 HF15K13r HF Hordeum vulgare subsp. v... 119 1e-026
>gb|AV927308.1|AV927308 AV927308 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd1h18 3', mRNA sequence
Length = 488
Score = 151 bits (324), Expect = 3e-036
Identities = 64/123 (52%), Positives = 86/123 (69%)
Frame = -2 / -2
Query: 512 TRAIFVHEADMAAVTVRAVDDPRALDKTLYLRPPANTCSLAHLVRLWEDKTGRALDKYYM 333
+RAIFV+ DM+ +T+RAV+DPR LDK L++RPPAN S LV L E KTG+ +++Y+
Sbjct: 481 SRAIFVNXKDMSMLTIRAVEDPRTLDKILHVRPPANMRSFGQLVHLLEKKTGKTFERHYV 302
Query: 332 PDEELVNRIRDSPLPLNFQLAMVHATVVAGVCDQTVDAEAGGVEATELYPDVNYVTVHDY 153
+ ELV +I+++P PLNFQLAMVH+TV + A VEAT LYPDV ++ V D
Sbjct: 301 TEPELVKKIQEAPFPLNFQLAMVHSTVAHAGACEHAVDPAVSVEATLLYPDVQFIKVEDC 122
Query: 152 LDG 144
LDG
Sbjct: 121 LDG 113
>gb|BM817239.1|BM817239 HC05A08_T3.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC05A08_T3.ab1 similar to 2'-hydroxyisoflavone
reductase,sulfur reductase,, mRNA sequence
Length = 866
Score = 119 bits (255), Expect(2) = 5e-034
Identities = 45/99 (45%), Positives = 67/99 (67%)
Frame = -2 / +3
Query: 539 RATVFGDDATRAIFVHEADMAAVTVRAVDDPRALDKTLYLRPPANTCSLAHLVRLWEDKT 360
+ + GD + +F E D+ T++AVDDPR L+K LYLRPP+NT S LV LWE K
Sbjct: 225 KVLIMGDGNVKGVFAAEEDVGTYTIKAVDDPRTLNKILYLRPPSNTLSHNELVSLWEKKL 404
Query: 359 GRALDKYYMPDEELVNRIRDSPLPLNFQLAMVHATVVAG 243
G+ L++ Y+P++EL+ +I++SP PLN LA+ H+ + G
Sbjct: 405 GKTLERVYLPEDELLKKIQESPAPLNVALAISHSVWLKG 521
Score = 44.1 bits (90), Expect(2) = 5e-034
Identities = 16/21 (76%), Positives = 20/21 (95%)
Frame = -2 / +3
Query: 209 GVEATELYPDVNYVTVHDYLD 147
GVEATELYPDV+Y+TV +YL+
Sbjct: 555 GVEATELYPDVHYITVDEYLN 617
>gb|BU987748.1|BU987748 HF15K13r HF Hordeum vulgare subsp. vulgare cDNA clone HF15K13
5-PRIME, mRNA sequence
Length = 475
Score = 119 bits (255), Expect = 1e-026
Identities = 45/99 (45%), Positives = 67/99 (67%)
Frame = -2 / +1
Query: 539 RATVFGDDATRAIFVHEADMAAVTVRAVDDPRALDKTLYLRPPANTCSLAHLVRLWEDKT 360
+ + GD + +F E D+ T++AVDDPR L+K LYLRPP+NT S LV LWE K
Sbjct: 139 KVLIMGDGNVKGVFAAEEDVGTYTIKAVDDPRTLNKILYLRPPSNTLSHNELVSLWEKKL 318
Query: 359 GRALDKYYMPDEELVNRIRDSPLPLNFQLAMVHATVVAG 243
G+ L++ Y+P++EL+ +I++SP PLN LA+ H+ + G
Sbjct: 319 GKTLERVYLPEDELLKKIQESPAPLNVALAISHSVWLKG 435
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 158,364,564
Number of Sequences: 312970
Number of extensions: 2559135
Number of successful extensions: 131062
Number of sequences better than 1.0e-020: 5
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 5512128591
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)