TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3067213.2.1
(924 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI780615.2|BI780615 EBes01_SQ001_H19_R embryo sac, 4-6 D... 407 e-113
gb|BJ473611.1|BJ473611 BJ473611 K. Sato unpublished cDNA li... 366 e-100
gb|BJ455168.1|BJ455168 BJ455168 K. Sato unpublished cDNA li... 364 e-100
gb|BJ453298.1|BJ453298 BJ453298 K. Sato unpublished cDNA li... 327 5e-089
gb|BI948762.1|BI948762 HVSMEl0010L20f Hordeum vulgare spike... 298 3e-080
gb|CV064134.1|CV064134 BNEL98d5 Barley EST endosperm librar... 277 5e-074
gb|BI957363.1|BI957363 HVSMEn0009C19f Hordeum vulgare rachi... 256 1e-067
gb|CV054787.1|CV054787 BNEL113c8 Barley EST endosperm libra... 248 2e-065
gb|BU997246.1|BU997246 HI07G08r HI Hordeum vulgare subsp. v... 245 3e-064
gb|BJ460841.1|BJ460841 BJ460841 K. Sato unpublished cDNA li... 231 6e-060
gb|CV061394.1|CV061394 BNEL68d1 Barley EST endosperm librar... 226 1e-058
gb|CV064122.1|CV064122 BNEL98c5 Barley EST endosperm librar... 226 1e-058
gb|CA014678.1|CA014678 HT12A16r HT Hordeum vulgare subsp. v... 207 9e-053
emb|AJ234443.1|HVU234443 Hordeum vulgare partial mRNA; clon... 185 4e-046
gb|BJ447422.1|BJ447422 BJ447422 K. Sato unpublished cDNA li... 165 9e-044
gb|CB858531.1|CB858531 HI07G08w HI Hordeum vulgare subsp. v... 131 5e-030
>gb|BI780615.2|BI780615 EBes01_SQ001_H19_R embryo sac, 4-6 DPA, no treatment, cv Optic,
EBes01 Hordeum vulgare subsp. vulgare cDNA clone
EBes01_SQ001_H19 5', mRNA sequence
Length = 527
Score = 407 bits (882), Expect = e-113
Identities = 165/175 (94%), Positives = 171/175 (97%)
Frame = -2 / +2
Query: 833 FPIAITFYITWWFIHFVDGFFSPIYAQLGINIFGLGFVTSVTFIFLIGVFMSSWLGASVL 654
+PIAITFY TWWFIHFVDGFFSPIYAQLGINIFGLGF+TSVTFIF +GVFMSSW+GASVL
Sbjct: 2 WPIAITFYFTWWFIHFVDGFFSPIYAQLGINIFGLGFITSVTFIFFVGVFMSSWVGASVL 181
Query: 653 SLGEWIIKRMPLVRHIYNASKQISAAISPDQNKQAFKEVVIIRHPRVGEYAFGFITSSVS 474
+GEWIIKRMPLVRHIYNASKQISAAISPDQNKQAFKE VIIRHPR+GEYAFGFITSSVS
Sbjct: 182 GIGEWIIKRMPLVRHIYNASKQISAAISPDQNKQAFKEAVIIRHPRIGEYAFGFITSSVS 361
Query: 473 LQSYSGQEDLYCVYVPTNHLYIGDIFMVNSKDVIRPNLSVREGIEIVVSGGMSMP 309
LQSYSGQEDLYCVYVPTNHLYIGDIFMVNSKDVIRPNLSVREGIEIVVSGGMSMP
Sbjct: 362 LQSYSGQEDLYCVYVPTNHLYIGDIFMVNSKDVIRPNLSVREGIEIVVSGGMSMP 526
Score = 337 bits (731), Expect = 4e-092
Identities = 147/175 (84%), Positives = 158/175 (90%)
Frame = +1 / -3
Query: 310 GIDIPPDTTISMPSRTERFGLMTSFEFTMKISPM*RWFVGT*TQ*RSS*PE*LWRDTDDV 489
GIDIPP+TTISMPSRTER GL+TSFEFTMKISP+*RW VGT TQ*RSS*PE*LWR+TD+V
Sbjct: 525 GIDIPPETTISMPSRTERLGLITSFEFTMKISPI*RWLVGTYTQ*RSS*PE*LWRETDEV 346
Query: 490 MKPNAYSPTRGCLMMTTSLNACLF*SGDIAALICFDAL*I*RTRGMRLIIHSPRLRTDAP 669
M PNAYSP RGC MMT SLNACLF*SGDIAALIC DAL*I RT GMRL+IHSP RTDAP
Sbjct: 345 MNPNAYSPIRGCRMMTASLNACLF*SGDIAALICLDAL*IWRTSGMRLMIHSPMPRTDAP 166
Query: 670 SQDDIKTPIKNMKVTDVTKPRPNMLIPS*A*IGEKNPSTK*MNHQVM*NVIAMGK 834
+QDD+KTP KNMKVT+V KPRP MLIP+ A*IGEKNPSTK*MNHQV *NVIAMG+
Sbjct: 165 TQDDMKTPTKNMKVTEVMKPRPKMLIPNCA*IGEKNPSTK*MNHQVK*NVIAMGQ 1
Score = 297 bits (642), Expect = 8e-080
Identities = 109/160 (68%), Positives = 129/160 (80%)
Frame = -1 / +1
Query: 789 FC*WILLSNLCSTGNQHIRSWLCHICYFHIFDRSLYVILAWGICP*PWRVDY*AHASCSS 610
FC*WILLSNLC+ GNQH SWL H CYFHIF SL+VIL W IC W +D+*AHA+C+
Sbjct: 46 FC*WILLSNLCTIGNQHFWSWLHHFCYFHIFRWSLHVILGWSICSWHW*MDH*AHATCAP 225
Query: 609 YLQCIEAN*CCNITRSEQTGVQGSGHHKASSCWRICVWFHHIVSIPPELFWSRRSLLRLC 430
YLQC++AN*CC ITRS+QTG+QG HH ASS W IC+W H+++S+ PELFWSR LL +C
Sbjct: 226 YLQCVQAN*CCYITRSKQTGIQGGSHHTASSYWGICIWIHYLISLSPELFWSRGPLLCVC 405
Query: 429 PNEPSLHW*YLHGEFKGCHKAKSFCA*RHRNCCIWWYVDA 310
PN+PSL+W*YLHGE KGC KA SFCA*RH + C WWYV+A
Sbjct: 406 PNQPSLYW*YLHGELKGCDKA*SFCA*RH*DRCFWWYVNA 525
Score = 290 bits (628), Expect = 7e-078
Identities = 124/174 (71%), Positives = 140/174 (80%)
Frame = +3 / -2
Query: 309 GHRHTTRYNNFDAFTHRKIWPYDIL*IHHEDITNVKMVRWDIDAVKIFLTRITLEGY*RC 488
GH HTTR N+ +AFTHRKI PY IL*+HHEDITN+KMV WDI VK+ LTRI LE *
Sbjct: 526 GH*HTTRNNDLNAFTHRKIRPYHIL*VHHEDITNIKMVGWDIHTVKVLLTRIALERD**G 347
Query: 489 DETKRIFSNTRMPYDDHFLERLFVLIW*YCSTNLLRCIVDMTNKRHALNNPLAKAKDRCP 668
+E+K IF NTRMPYDD LE LFVLIW*Y STNLL IVDM +K HALN+P AK+RC
Sbjct: 346 NESKCIFPNTRMPYDDCLLECLFVLIW*YSSTNLLGRIVDMAHKWHALNDPFTNAKNRCS 167
Query: 669 KPR*HKDSDQKYESNRCDKAKTEYVDSQLSIDWREESINKMNEPPGDVERYCYG 830
PR*H+DS++KYESNR D+AKT+ VDSQL IDWREESINKMNEPPG+VE Y YG
Sbjct: 166 NPR*HEDSNEKYESNRSDEAKTKNVDSQLCIDWREESINKMNEPPGEVECYRYG 5
Score = 226 bits (489), Expect = 1e-058
Identities = 113/162 (69%), Positives = 122/162 (75%)
Frame = +2 / -1
Query: 308 GASTYHQIQQFRCLHAQKDLAL*HPLNSP*RYHQCKDGSLGHRRSKDLLDQNNSGGILTM 487
GA TYHQ Q+ +CLHAQKD AL HPL+SP*RYHQ KDG LGH SK LDQN+SG L
Sbjct: 527 GALTYHQKQRSQCLHAQKD*ALSHPLSSP*RYHQYKDGWLGHTHSKGPLDQNSSGERLMR 348
Query: 488 **NQTHILQHEDAL**PLP*TPVCSDLVILQH*FASMHCRYDEQEACA**STRQG*GQMP 667
** Q HI Q+EDA+** P* PVC DLVI QH*FA HCRY Q ACA**S Q QM
Sbjct: 347 **IQMHIPQYEDAV**LPP*MPVCFDLVI*QH*FAWTHCRYGAQVACA**SIHQCQEQML 168
Query: 668 QAKMT*RLRSKI*K*QM*QSQDRIC*FPVEHRLERRIHQQNE 793
Q KMT*RL+ KI*K*Q * SQD+ C*FP+ HRLERRIHQQNE
Sbjct: 167 QPKMT*RLQRKI*K*QK**SQDQKC*FPIVHRLERRIHQQNE 42
Score = 145 bits (311), Expect(2) = 2e-052
Identities = 70/101 (69%), Positives = 76/101 (75%)
Frame = -3 / +3
Query: 610 ISTMHRSKLVLQYHQIRTNRRSRKWSS*GILVLENMRLVSSHRQYPSRVILVKKIFTASM 431
ISTM SKLVL YHQI+TNR SR+ SS GILVL NM L S Q SR ILVK+ FT M
Sbjct: 225 ISTMRPSKLVLLYHQIKTNRHSRRQSSYGILVLGNMHLDSLPHQSLSRAILVKRTFTVCM 404
Query: 430 SQRTIFTLVISSW*IQRMS*GQIFLCVKASKLLYLVVCRCP 308
SQ TIF LVISSW* QRM *G IFLCVKA + L+LVVC+CP
Sbjct: 405 SQPTIFILVISSW*TQRM**GLIFLCVKALRSLFLVVCQCP 527
Score = 80.8 bits (170), Expect(2) = 2e-052
Identities = 37/44 (84%), Positives = 39/44 (88%)
Frame = -3 / +3
Query: 829 P*Q*RSTSPGGSFILLMDSSLQSMLNWESTYSVLALSHLLLSYF 698
P*+* STSPGGSFILLMDSSLQSM NWEST+ VLA S LLLSYF
Sbjct: 6 P*R*HSTSPGGSFILLMDSSLQSMHNWESTFLVLASSLLLLSYF 137
>gb|BJ473611.1|BJ473611 BJ473611 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal15k07 3', mRNA sequence
Length = 699
Score = 366 bits (793), Expect = e-100
Identities = 152/160 (95%), Positives = 157/160 (98%)
Frame = -2 / -1
Query: 764 IYAQLGINIFGLGFVTSVTFIFLIGVFMSSWLGASVLSLGEWIIKRMPLVRHIYNASKQI 585
IYAQLGINIFGLGF+TSVTFIF +GVFMSSW+GASVL +GEWIIKRMPLVRHIYNASKQI
Sbjct: 699 IYAQLGINIFGLGFITSVTFIFFVGVFMSSWVGASVLGIGEWIIKRMPLVRHIYNASKQI 520
Query: 584 SAAISPDQNKQAFKEVVIIRHPRVGEYAFGFITSSVSLQSYSGQEDLYCVYVPTNHLYIG 405
SAAISPDQNKQAFKE VIIRHPR+GEYAFGFITSSVSLQSYSGQEDLYCVYVPTNHLYIG
Sbjct: 519 SAAISPDQNKQAFKEAVIIRHPRIGEYAFGFITSSVSLQSYSGQEDLYCVYVPTNHLYIG 340
Query: 404 DIFMVNSKDVIRPNLSVREGIEIVVSGGMSMPQILSTLDP 285
DIFMVNSKDVIRPNLSVREGIEIVVSGGMSMPQILSTLDP
Sbjct: 339 DIFMVNSKDVIRPNLSVREGIEIVVSGGMSMPQILSTLDP 220
Score = 312 bits (676), Expect = 2e-084
Identities = 133/160 (83%), Positives = 144/160 (90%)
Frame = +1 / +2
Query: 283 CGSRVDRIWGIDIPPDTTISMPSRTERFGLMTSFEFTMKISPM*RWFVGT*TQ*RSS*PE 462
CGSRVD+IWGIDIPP+TTISMPSRTER GL+TSFEFTMKISP+*RW VGT TQ*RSS*PE
Sbjct: 218 CGSRVDKIWGIDIPPETTISMPSRTERLGLITSFEFTMKISPI*RWLVGTYTQ*RSS*PE 397
Query: 463 *LWRDTDDVMKPNAYSPTRGCLMMTTSLNACLF*SGDIAALICFDAL*I*RTRGMRLIIH 642
*LWR+TD+VM PNAYSP RGC MMT SLNACLF*SGDIAALIC DAL*I RT GMRL+IH
Sbjct: 398 *LWRETDEVMNPNAYSPIRGCRMMTASLNACLF*SGDIAALICLDAL*IWRTSGMRLMIH 577
Query: 643 SPRLRTDAPSQDDIKTPIKNMKVTDVTKPRPNMLIPS*A* 762
SP RTDAP+QDD+KTP KNMKVT+V KPRP MLIP+ A*
Sbjct: 578 SPMPRTDAPTQDDMKTPTKNMKVTEVMKPRPKMLIPNCA* 697
Score = 286 bits (618), Expect = 2e-076
Identities = 105/160 (65%), Positives = 126/160 (78%)
Frame = -1 / -3
Query: 762 LCSTGNQHIRSWLCHICYFHIFDRSLYVILAWGICP*PWRVDY*AHASCSSYLQCIEAN* 583
LC+ GNQH SWL H CYFHIF SL+VIL W IC W +D+*AHA+C+ YLQC++AN*
Sbjct: 697 LCTIGNQHFWSWLHHFCYFHIFRWSLHVILGWSICSWHW*MDH*AHATCAPYLQCVQAN* 518
Query: 582 CCNITRSEQTGVQGSGHHKASSCWRICVWFHHIVSIPPELFWSRRSLLRLCPNEPSLHW* 403
CC ITRS+QTG+QG HH ASS W IC+W H+++S+ PELFWSR LL +CPN+PSL+W*
Sbjct: 517 CCYITRSKQTGIQGGSHHTASSYWGICIWIHYLISLSPELFWSRGPLLCVCPNQPSLYW* 338
Query: 402 YLHGEFKGCHKAKSFCA*RHRNCCIWWYVDAPNSVNPRPT 283
YLHGE KGC KA SFCA*RH + C WWYV+AP+ VNP T
Sbjct: 337 YLHGELKGCDKA*SFCA*RH*DRCFWWYVNAPDFVNPGST 218
Score = 260 bits (562), Expect = 9e-069
Identities = 111/161 (68%), Positives = 129/161 (80%)
Frame = +3 / +1
Query: 282 LWVEG*QNLGHRHTTRYNNFDAFTHRKIWPYDIL*IHHEDITNVKMVRWDIDAVKIFLTR 461
+W++G*QNLGH HTTR N+ +AFTHRKI PY IL*+HHEDITN+KMV WDI VK+ LTR
Sbjct: 217 MWIQG*QNLGH*HTTRNNDLNAFTHRKIRPYHIL*VHHEDITNIKMVGWDIHTVKVLLTR 396
Query: 462 ITLEGY*RCDETKRIFSNTRMPYDDHFLERLFVLIW*YCSTNLLRCIVDMTNKRHALNNP 641
I LE * +E+K IF NTRMPYDD LE LFVLIW*Y STNLL IVDM +K HALN+P
Sbjct: 397 IALERD**GNESKCIFPNTRMPYDDCLLECLFVLIW*YSSTNLLGRIVDMAHKWHALNDP 576
Query: 642 LAKAKDRCPKPR*HKDSDQKYESNRCDKAKTEYVDSQLSID 764
AK+RC PR*H+DS++KYESNR D+AKT+ VDSQL ID
Sbjct: 577 FTNAKNRCSNPR*HEDSNEKYESNRSDEAKTKNVDSQLCID 699
Score = 210 bits (452), Expect = 1e-053
Identities = 107/160 (66%), Positives = 117/160 (73%)
Frame = +2 / +3
Query: 284 VGRGLTEFGASTYHQIQQFRCLHAQKDLAL*HPLNSP*RYHQCKDGSLGHRRSKDLLDQN 463
V GLT+ GA TYHQ Q+ +CLHAQKD AL HPL+SP*RYHQ KDG LGH SK LDQN
Sbjct: 219 VDPGLTKSGALTYHQKQRSQCLHAQKD*ALSHPLSSP*RYHQYKDGWLGHTHSKGPLDQN 398
Query: 464 NSGGILTM**NQTHILQHEDAL**PLP*TPVCSDLVILQH*FASMHCRYDEQEACA**ST 643
+SG L ** Q HI Q+EDA+** P* PVC DLVI QH*FA HCRY Q ACA**S
Sbjct: 399 SSGERLMR**IQMHIPQYEDAV**LPP*MPVCFDLVI*QH*FAWTHCRYGAQVACA**SI 578
Query: 644 RQG*GQMPQAKMT*RLRSKI*K*QM*QSQDRIC*FPVEHR 763
Q QM Q KMT*RL+ KI*K*Q * SQD+ C*FP+ HR
Sbjct: 579 HQCQEQMLQPKMT*RLQRKI*K*QK**SQDQKC*FPIVHR 698
Score = 160 bits (344), Expect(2) = 3e-044
Identities = 75/109 (68%), Positives = 82/109 (75%)
Frame = -3 / -2
Query: 610 ISTMHRSKLVLQYHQIRTNRRSRKWSS*GILVLENMRLVSSHRQYPSRVILVKKIFTASM 431
ISTM SKLVL YHQI+TNR SR+ SS GILVL NM L S Q SR ILVK+ FT M
Sbjct: 545 ISTMRPSKLVLLYHQIKTNRHSRRQSSYGILVLGNMHLDSLPHQSLSRAILVKRTFTVCM 366
Query: 430 SQRTIFTLVISSW*IQRMS*GQIFLCVKASKLLYLVVCRCPKFCQPSTH 284
SQ TIF LVISSW* QRM *G IFLCVKA + L+LVVC+CP+FCQP H
Sbjct: 365 SQPTIFILVISSW*TQRM**GLIFLCVKALRSLFLVVCQCPRFCQPWIH 219
Score = 38.6 bits (78), Expect(2) = 3e-044
Identities = 17/22 (77%), Positives = 18/22 (81%)
Frame = -3 / -2
Query: 763 SMLNWESTYSVLALSHLLLSYF 698
SM NWEST+ VLA S LLLSYF
Sbjct: 698 SMHNWESTFLVLASSLLLLSYF 633
>gb|BJ455168.1|BJ455168 BJ455168 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak35h23 3', mRNA sequence
Length = 697
Score = 364 bits (789), Expect = e-100
Identities = 151/159 (94%), Positives = 156/159 (98%)
Frame = -2 / -2
Query: 761 YAQLGINIFGLGFVTSVTFIFLIGVFMSSWLGASVLSLGEWIIKRMPLVRHIYNASKQIS 582
YAQLGINIFGLGF+TSVTFIF +GVFMSSW+GASVL +GEWIIKRMPLVRHIYNASKQIS
Sbjct: 696 YAQLGINIFGLGFITSVTFIFFVGVFMSSWVGASVLGIGEWIIKRMPLVRHIYNASKQIS 517
Query: 581 AAISPDQNKQAFKEVVIIRHPRVGEYAFGFITSSVSLQSYSGQEDLYCVYVPTNHLYIGD 402
AAISPDQNKQAFKE VIIRHPR+GEYAFGFITSSVSLQSYSGQEDLYCVYVPTNHLYIGD
Sbjct: 516 AAISPDQNKQAFKEAVIIRHPRIGEYAFGFITSSVSLQSYSGQEDLYCVYVPTNHLYIGD 337
Query: 401 IFMVNSKDVIRPNLSVREGIEIVVSGGMSMPQILSTLDP 285
IFMVNSKDVIRPNLSVREGIEIVVSGGMSMPQILSTLDP
Sbjct: 336 IFMVNSKDVIRPNLSVREGIEIVVSGGMSMPQILSTLDP 220
Score = 312 bits (676), Expect = 2e-084
Identities = 133/160 (83%), Positives = 144/160 (90%)
Frame = +1 / +2
Query: 283 CGSRVDRIWGIDIPPDTTISMPSRTERFGLMTSFEFTMKISPM*RWFVGT*TQ*RSS*PE 462
CGSRVD+IWGIDIPP+TTISMPSRTER GL+TSFEFTMKISP+*RW VGT TQ*RSS*PE
Sbjct: 218 CGSRVDKIWGIDIPPETTISMPSRTERLGLITSFEFTMKISPI*RWLVGTYTQ*RSS*PE 397
Query: 463 *LWRDTDDVMKPNAYSPTRGCLMMTTSLNACLF*SGDIAALICFDAL*I*RTRGMRLIIH 642
*LWR+TD+VM PNAYSP RGC MMT SLNACLF*SGDIAALIC DAL*I RT GMRL+IH
Sbjct: 398 *LWRETDEVMNPNAYSPIRGCRMMTASLNACLF*SGDIAALICLDAL*IWRTSGMRLMIH 577
Query: 643 SPRLRTDAPSQDDIKTPIKNMKVTDVTKPRPNMLIPS*A* 762
SP RTDAP+QDD+KTP KNMKVT+V KPRP MLIP+ A*
Sbjct: 578 SPMPRTDAPTQDDMKTPTKNMKVTEVMKPRPKMLIPNCA* 697
Score = 286 bits (618), Expect = 2e-076
Identities = 105/160 (65%), Positives = 126/160 (78%)
Frame = -1 / -1
Query: 762 LCSTGNQHIRSWLCHICYFHIFDRSLYVILAWGICP*PWRVDY*AHASCSSYLQCIEAN* 583
LC+ GNQH SWL H CYFHIF SL+VIL W IC W +D+*AHA+C+ YLQC++AN*
Sbjct: 697 LCTIGNQHFWSWLHHFCYFHIFRWSLHVILGWSICSWHW*MDH*AHATCAPYLQCVQAN* 518
Query: 582 CCNITRSEQTGVQGSGHHKASSCWRICVWFHHIVSIPPELFWSRRSLLRLCPNEPSLHW* 403
CC ITRS+QTG+QG HH ASS W IC+W H+++S+ PELFWSR LL +CPN+PSL+W*
Sbjct: 517 CCYITRSKQTGIQGGSHHTASSYWGICIWIHYLISLSPELFWSRGPLLCVCPNQPSLYW* 338
Query: 402 YLHGEFKGCHKAKSFCA*RHRNCCIWWYVDAPNSVNPRPT 283
YLHGE KGC KA SFCA*RH + C WWYV+AP+ VNP T
Sbjct: 337 YLHGELKGCDKA*SFCA*RH*DRCFWWYVNAPDFVNPGST 218
Score = 257 bits (556), Expect = 6e-068
Identities = 110/160 (68%), Positives = 128/160 (80%)
Frame = +3 / +1
Query: 282 LWVEG*QNLGHRHTTRYNNFDAFTHRKIWPYDIL*IHHEDITNVKMVRWDIDAVKIFLTR 461
+W++G*QNLGH HTTR N+ +AFTHRKI PY IL*+HHEDITN+KMV WDI VK+ LTR
Sbjct: 217 MWIQG*QNLGH*HTTRNNDLNAFTHRKIRPYHIL*VHHEDITNIKMVGWDIHTVKVLLTR 396
Query: 462 ITLEGY*RCDETKRIFSNTRMPYDDHFLERLFVLIW*YCSTNLLRCIVDMTNKRHALNNP 641
I LE * +E+K IF NTRMPYDD LE LFVLIW*Y STNLL IVDM +K HALN+P
Sbjct: 397 IALERD**GNESKCIFPNTRMPYDDCLLECLFVLIW*YSSTNLLGRIVDMAHKWHALNDP 576
Query: 642 LAKAKDRCPKPR*HKDSDQKYESNRCDKAKTEYVDSQLSI 761
AK+RC PR*H+DS++KYESNR D+AKT+ VDSQL I
Sbjct: 577 FTNAKNRCSNPR*HEDSNEKYESNRSDEAKTKNVDSQLCI 696
Score = 207 bits (447), Expect = 6e-053
Identities = 106/159 (66%), Positives = 116/159 (72%)
Frame = +2 / +3
Query: 284 VGRGLTEFGASTYHQIQQFRCLHAQKDLAL*HPLNSP*RYHQCKDGSLGHRRSKDLLDQN 463
V GLT+ GA TYHQ Q+ +CLHAQKD AL HPL+SP*RYHQ KDG LGH SK LDQN
Sbjct: 219 VDPGLTKSGALTYHQKQRSQCLHAQKD*ALSHPLSSP*RYHQYKDGWLGHTHSKGPLDQN 398
Query: 464 NSGGILTM**NQTHILQHEDAL**PLP*TPVCSDLVILQH*FASMHCRYDEQEACA**ST 643
+SG L ** Q HI Q+EDA+** P* PVC DLVI QH*FA HCRY Q ACA**S
Sbjct: 399 SSGERLMR**IQMHIPQYEDAV**LPP*MPVCFDLVI*QH*FAWTHCRYGAQVACA**SI 578
Query: 644 RQG*GQMPQAKMT*RLRSKI*K*QM*QSQDRIC*FPVEH 760
Q QM Q KMT*RL+ KI*K*Q * SQD+ C*FP+ H
Sbjct: 579 HQCQEQMLQPKMT*RLQRKI*K*QK**SQDQKC*FPIVH 695
Score = 160 bits (344), Expect(2) = 1e-043
Identities = 75/109 (68%), Positives = 82/109 (75%)
Frame = -3 / -3
Query: 610 ISTMHRSKLVLQYHQIRTNRRSRKWSS*GILVLENMRLVSSHRQYPSRVILVKKIFTASM 431
ISTM SKLVL YHQI+TNR SR+ SS GILVL NM L S Q SR ILVK+ FT M
Sbjct: 545 ISTMRPSKLVLLYHQIKTNRHSRRQSSYGILVLGNMHLDSLPHQSLSRAILVKRTFTVCM 366
Query: 430 SQRTIFTLVISSW*IQRMS*GQIFLCVKASKLLYLVVCRCPKFCQPSTH 284
SQ TIF LVISSW* QRM *G IFLCVKA + L+LVVC+CP+FCQP H
Sbjct: 365 SQPTIFILVISSW*TQRM**GLIFLCVKALRSLFLVVCQCPRFCQPWIH 219
Score = 36.8 bits (74), Expect(2) = 1e-043
Identities = 16/21 (76%), Positives = 17/21 (80%)
Frame = -3 / -3
Query: 760 MLNWESTYSVLALSHLLLSYF 698
M NWEST+ VLA S LLLSYF
Sbjct: 695 MHNWESTFLVLASSLLLLSYF 633
>gb|BJ453298.1|BJ453298 BJ453298 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak42g23 5', mRNA sequence
Length = 633
Score = 327 bits (709), Expect = 5e-089
Identities = 123/178 (69%), Positives = 157/178 (88%)
Frame = -2 / +2
Query: 902 REAFHKVVRSWASKKFMTGCVILFPIAITFYITWWFIHFVDGFFSPIYAQLGINIFGLGF 723
R+A V++SW S+KFMTGCV+LFP+A TF+ITWWF+ FVDGFFSP+YA++G++IFGLGF
Sbjct: 98 RKACCAVLQSWVSRKFMTGCVVLFPVAFTFFITWWFVQFVDGFFSPLYAKIGVDIFGLGF 277
Query: 722 VTSVTFIFLIGVFMSSWLGASVLSLGEWIIKRMPLVRHIYNASKQISAAISPDQNKQAFK 543
+TS+ FIFL+G+F+SSW+G++V +GEW IK+MP V+HIY+ASKQ+S AISPDQN AFK
Sbjct: 278 LTSLAFIFLVGIFVSSWVGSTVFWVGEWFIKKMPFVKHIYSASKQVSTAISPDQNTTAFK 457
Query: 542 EVVIIRHPRVGEYAFGFITSSVSLQSYSGQEDLYCVYVPTNHLYIGDIFMVNSKDVIR 369
EV IIRHPRVGEYAFGFITS+V LQ+ G E+L VYVPTNHLYIGDIF++NS+++IR
Sbjct: 458 EVAIIRHPRVGEYAFGFITSTVVLQTDKGDEELCSVYVPTNHLYIGDIFLLNSEEIIR 631
Score = 203 bits (438), Expect = 1e-051
Identities = 98/179 (54%), Positives = 116/179 (64%)
Frame = +1 / -1
Query: 367 GLMTSFEFTMKISPM*RWFVGT*TQ*RSS*PE*LWRDTDDVMKPNAYSPTRGCLMMTTSL 546
GLM S EF+ ISP+ R FVGT*T SS P +WR T DV+ PNAYSPTRGC ++ TSL
Sbjct: 633 GLMISSEFSRNISPIYR*FVGT*TLHNSSSPLSVWRTTVDVINPNAYSPTRGCRIIATSL 454
Query: 547 NACLF*SGDIAALICFDAL*I*RTRGMRLIIHSPRLRTDAPSQDDIKTPIKNMKVTDVTK 726
NA +F*SG++A L CFDA + T G+ I HSP +T P+ ++ P +NM DV K
Sbjct: 453 NAVVF*SGEMAVLTCFDAEYMCFTNGIFFINHSPTQKTVEPTHEETNIPTRNMNARDVRK 274
Query: 727 PRPNMLIPS*A*IGEKNPSTK*MNHQVM*NVIAMGKRITHPVMNFFEAQLRTTLWKASR 903
PRPNM P A G KNPST * NH VM NV A GKR THPV+NF + QL T A R
Sbjct: 273 PRPNMSTPIFAYSGLKNPSTN*TNHHVMKNVKATGKRTTHPVINFLDTQL*RTAQHALR 97
Score = 132 bits (282), Expect = 4e-030
Identities = 67/176 (38%), Positives = 98/176 (55%)
Frame = +3 / -3
Query: 375 DIL*IHHEDITNVKMVRWDIDAVKIFLTRITLEGY*RCDETKRIFSNTRMPYDDHFLERL 554
D+ + + ITN+ M+ I+ + +T + LE C +++ +F+NT M + +
Sbjct: 625 DLFRVQQKYITNI*MICRHINTTQFLITFVCLENNS*CYKSECVFTNTWMSNYRNLFKCC 446
Query: 555 FVLIW*YCSTNLLRCIVDMTNKRHALNNPLAKAKDRCPKPR*HKDSDQKYESNRCDKAKT 734
+LIW* S +LL C + + K H L P + +D P +K S+QKYE RC K KT
Sbjct: 445 RILIW*NGSADLL*CRIYVLYKWHFLYKPFSNPEDS*THP*RNKYSNQKYECQRCQKTKT 266
Query: 735 EYVDSQLSIDWREESINKMNEPPGDVERYCYGEENYTSSHELL*SPTADNLMESLA 902
+YVDS I W E+SINK+NEPP D ER ++N+TSSH+ PT N LA
Sbjct: 265 KYVDSDFCI*WAEKSINKLNEPPCDEERESNRKKNHTSSHKFSRYPTLKNSAACLA 98
>gb|BI948762.1|BI948762 HVSMEl0010L20f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0010L20f, mRNA sequence
Length = 834
Score = 298 bits (645), Expect = 3e-080
Identities = 115/171 (67%), Positives = 146/171 (85%)
Frame = -2 / +2
Query: 797 FIHFVDGFFSPIYAQLGINIFGLGFVTSVTFIFLIGVFMSSWLGASVLSLGEWIIKRMPL 618
FI DGFFSP+YA+LG ++FGLGFVTS+ FIF++G+F+SSW+G++V +GEW I++MP
Sbjct: 2 FIRSFDGFFSPLYAKLGFDVFGLGFVTSLVFIFIVGIFVSSWVGSTVFWVGEWFIEKMPF 181
Query: 617 VRHIYNASKQISAAISPDQNKQAFKEVVIIRHPRVGEYAFGFITSSVSLQSYSGQEDLYC 438
VRHIY+ASKQ+S A+SPDQN AFKEV II HPR GEYAFGFITSS+ LQ+ G E+L
Sbjct: 182 VRHIYSASKQVSTAVSPDQNTTAFKEVAIISHPRAGEYAFGFITSSMILQTDKGDEELCS 361
Query: 437 VYVPTNHLYIGDIFMVNSKDVIRPNLSVREGIEIVVSGGMSMPQILSTLDP 285
VYVPTNHL IGDIF+VNS ++IRPNLS+REGIEI+VSGGM+MP++++ L P
Sbjct: 362 VYVPTNHLCIGDIFLVNSAEIIRPNLSIREGIEIIVSGGMTMPEVITALGP 514
Score = 167 bits (360), Expect = 6e-041
Identities = 86/163 (52%), Positives = 103/163 (63%)
Frame = +1 / -1
Query: 310 GIDIPPDTTISMPSRTERFGLMTSFEFTMKISPM*RWFVGT*TQ*RSS*PE*LWRDTDDV 489
G+ IPP+T IS+PSR +R GL+ S EFT KISP+ R VGT*T SS P + R DV
Sbjct: 489 GMVIPPETIISIPSRMDRLGLIISAEFTKKISPIHR*LVGT*TLHSSSSPLSV*RIMLDV 310
Query: 490 MKPNAYSPTRGCLMMTTSLNACLF*SGDIAALICFDAL*I*RTRGMRLIIHSPRLRTDAP 669
+ PNAYSP RG L++ TSLNA +F*SG+ A L CFDA + T G+ I HSP +T P
Sbjct: 309 INPNAYSPARGWLIIATSLNAVVF*SGETAVLTCFDAEYMCLTNGIFSINHSPTQKTVEP 130
Query: 670 SQDDIKTPIKNMKVTDVTKPRPNMLIPS*A*IGEKNPSTK*MN 798
+ DD P NM DVT PRPN PS A* G K PS +N
Sbjct: 129 THDDTNIPTINMNTRDVTNPRPNTSNPSFA*SGLKKPSKDRIN 1
Score = 144 bits (308), Expect = 9e-034
Identities = 61/165 (36%), Positives = 100/165 (60%)
Frame = +3 / -3
Query: 303 NLGHRHTTRYNNFDAFTHRKIWPYDIL*IHHEDITNVKMVRWDIDAVKIFLTRITLEGY* 482
+L H H++R N F++ ++ PY++ +H +DITN M+ W ID + F+T ++L+ +
Sbjct: 496 HLRHGHSSRNNYLYPFSNGQVGPYNLCRVHQKDITNT*MIGWHIDTTQFFITFVSLKNHA 317
Query: 483 RCDETKRIFSNTRMPYDDHFLERLFVLIW*YCSTNLLRCIVDMTNKRHALNNPLAKAKDR 662
RC ++K +F++T M HF + +LIW* S NLL C + + K H L P + +D
Sbjct: 316 RCYKSKCVFTSTGMANYCHFFKCCCILIW*NSSANLL*CRIYVPYKWHFLYKPFSNPEDS 137
Query: 663 CPKPR*HKDSDQKYESNRCDKAKTEYVDSQLSIDWREESINKMNE 797
P *H+ S+ KYE RC K K +YV+S+L I+W E+++ N+
Sbjct: 136 *AHP**HEYSNNKYEYKRCHKPKAKYVESKLCIEWTEKTVEGSNK 2
>gb|CV064134.1|CV064134 BNEL98d5 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL98d5 5' similar to unknown
protein, mRNA sequence
Length = 649
Score = 277 bits (600), Expect = 5e-074
Identities = 105/159 (66%), Positives = 134/159 (84%)
Frame = -2 / +2
Query: 902 REAFHKVVRSWASKKFMTGCVILFPIAITFYITWWFIHFVDGFFSPIYAQLGINIFGLGF 723
R+A V++SW S+KFMTGCV++FP+A+TF+IT WFI F DGFFSP+YA+LG ++FGLGF
Sbjct: 173 RKACFAVLQSWVSRKFMTGCVVIFPMAVTFFITLWFIRFFDGFFSPLYAKLGFDVFGLGF 352
Query: 722 VTSVTFIFLIGVFMSSWLGASVLSLGEWIIKRMPLVRHIYNASKQISAAISPDQNKQAFK 543
VTS+ FIF++G+F+SSW+G++V +GEW IK+MP VRHIY+ASKQ+S A+SPDQN AFK
Sbjct: 353 VTSLVFIFIVGIFVSSWVGSTVFWVGEWFIKKMPFVRHIYSASKQVSTAVSPDQNTTAFK 532
Query: 542 EVVIIRHPRVGEYAFGFITSSVSLQSYSGQEDLYCVYVP 426
EV II HPR GEYAFGFITSS+ LQ+ G E+L VYVP
Sbjct: 533 EVAIISHPRAGEYAFGFITSSMILQTDKGDEELCSVYVP 649
Score = 158 bits (340), Expect = 4e-038
Identities = 82/159 (51%), Positives = 97/159 (61%)
Frame = +1 / -2
Query: 427 GT*TQ*RSS*PE*LWRDTDDVMKPNAYSPTRGCLMMTTSLNACLF*SGDIAALICFDAL* 606
GT*T SS P + R DV+ PNAYSP RG L++ TSLNA +F*SG+ A L CFDA
Sbjct: 648 GT*TLHSSSSPLSV*RIMLDVINPNAYSPARGWLIIATSLNAVVF*SGETAVLTCFDAEY 469
Query: 607 I*RTRGMRLIIHSPRLRTDAPSQDDIKTPIKNMKVTDVTKPRPNMLIPS*A*IGEKNPST 786
+ T G+ I HSP +T P+ DD P NM DVT PRPN PS A* G K PS
Sbjct: 468 MCLTNGIFFINHSPTQKTVEPTHDDTNIPTINMNTRDVTNPRPNTSNPSFA*SGLKKPSK 289
Query: 787 K*MNHQVM*NVIAMGKRITHPVMNFFEAQLRTTLWKASR 903
+NH+VM NV A+GK THPV+NF + QL T A R
Sbjct: 288 NRINHKVMKNVTAIGKITTHPVINFLDTQL*RTAKHALR 172
Score = 138 bits (296), Expect = 4e-032
Identities = 60/149 (40%), Positives = 91/149 (61%)
Frame = +3 / -1
Query: 426 WDIDAVKIFLTRITLEGY*RCDETKRIFSNTRMPYDDHFLERLFVLIW*YCSTNLLRCIV 605
W ID + F+T ++L+ + RC ++K +F++T M HF + +LIW* S NLL C +
Sbjct: 649 WHIDTTQFFITFVSLKNHARCYKSKCVFTSTGMANYCHFFKCCCILIW*NSSANLL*CRI 470
Query: 606 DMTNKRHALNNPLAKAKDRCPKPR*HKDSDQKYESNRCDKAKTEYVDSQLSIDWREESIN 785
+ K H L P + +D P *H+ S+ KYE RC K K +YV+S+L I+W E+++
Sbjct: 469 YVPYKWHFLYKPFSNPEDS*AHP**HEYSNNKYEYKRCHKPKAKYVESKLCIEWTEKTVE 290
Query: 786 KMNEPPGDVERYCYGEENYTSSHELL*SP 872
+ N+P D E + + E NYTSSH+L * P
Sbjct: 289 ESNKPQSDEECHSHWENNYTSSHKLS*HP 203
>gb|BI957363.1|BI957363 HVSMEn0009C19f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0009C19f, mRNA sequence
Length = 656
Score = 256 bits (554), Expect = 1e-067
Identities = 95/140 (67%), Positives = 121/140 (86%)
Frame = -2 / +3
Query: 902 REAFHKVVRSWASKKFMTGCVILFPIAITFYITWWFIHFVDGFFSPIYAQLGINIFGLGF 723
R+A V++SW S+KFMTGCV++FP+A+TF+IT WFI F DGFFSP+YA+LG ++FGLGF
Sbjct: 192 RKACFAVLQSWVSRKFMTGCVVIFPMAVTFFITLWFIRFFDGFFSPLYAKLGFDVFGLGF 371
Query: 722 VTSVTFIFLIGVFMSSWLGASVLSLGEWIIKRMPLVRHIYNASKQISAAISPDQNKQAFK 543
VTS+ FIF++G+F+SSW+G++V +GEW IK+MP VRHIY+ASKQ+S A+SPDQN AFK
Sbjct: 372 VTSLVFIFIVGIFVSSWVGSTVFWVGEWFIKKMPFVRHIYSASKQVSTAVSPDQNTTAFK 551
Query: 542 EVVIIRHPRVGEYAFGFITS 483
EV II HPR GEYAFGFITS
Sbjct: 552 EVAIISHPRAGEYAFGFITS 611
Score = 155 bits (332), Expect = 5e-037
Identities = 74/140 (52%), Positives = 88/140 (62%)
Frame = +1 / -2
Query: 484 DVMKPNAYSPTRGCLMMTTSLNACLF*SGDIAALICFDAL*I*RTRGMRLIIHSPRLRTD 663
DV+ PNAYSP RG L++ TSLNA +F*SG+ A L CFDA + T G+ I HSP +T
Sbjct: 610 DVINPNAYSPARGWLIIATSLNAVVF*SGETAVLTCFDAEYMCLTNGIFFINHSPTQKTV 431
Query: 664 APSQDDIKTPIKNMKVTDVTKPRPNMLIPS*A*IGEKNPSTK*MNHQVM*NVIAMGKRIT 843
P+ DD P NM DVT PRPN PS A* G K PS +NH+VM NV A+GK T
Sbjct: 430 EPTHDDTNIPTINMNTRDVTNPRPNTSNPSFA*SGLKKPSKNRINHKVMKNVTAIGKITT 251
Query: 844 HPVMNFFEAQLRTTLWKASR 903
HPV+NF + QL T A R
Sbjct: 250 HPVINFLDTQL*RTAKHALR 191
Score = 118 bits (253), Expect = 4e-026
Identities = 53/130 (40%), Positives = 79/130 (60%)
Frame = +3 / -1
Query: 483 RCDETKRIFSNTRMPYDDHFLERLFVLIW*YCSTNLLRCIVDMTNKRHALNNPLAKAKDR 662
RC ++K +F++T M HF + +LIW* S NLL C + + K H L P + +D
Sbjct: 611 RCYKSKCVFTSTGMANYCHFFKCCCILIW*NSSANLL*CRIYVPYKWHFLYKPFSNPEDS 432
Query: 663 CPKPR*HKDSDQKYESNRCDKAKTEYVDSQLSIDWREESINKMNEPPGDVERYCYGEENY 842
P *H+ S+ KYE RC K K +YV+S+L I+W E+++ + N+P D E + + E N+
Sbjct: 431 *AHP**HEYSNNKYEYKRCHKPKAKYVESKLCIEWTEKTVEESNKPQSDEECHSHWENNH 252
Query: 843 TSSHELL*SP 872
TSSH+L * P
Sbjct: 251 TSSHKLS*HP 222
>gb|CV054787.1|CV054787 BNEL113c8 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL113c8 5' similar to P0677H08.7,
mRNA sequence
Length = 588
Score = 248 bits (537), Expect = 2e-065
Identities = 91/132 (68%), Positives = 117/132 (88%)
Frame = -2 / +3
Query: 902 REAFHKVVRSWASKKFMTGCVILFPIAITFYITWWFIHFVDGFFSPIYAQLGINIFGLGF 723
R+A V++SW S+KFMTGCV+LFP+A TF+ITWWF+ FVDGFFSP+YA++G++IFGLGF
Sbjct: 192 RKACCAVLQSWVSRKFMTGCVVLFPVAFTFFITWWFVQFVDGFFSPLYAKIGVDIFGLGF 371
Query: 722 VTSVTFIFLIGVFMSSWLGASVLSLGEWIIKRMPLVRHIYNASKQISAAISPDQNKQAFK 543
+TS+ FIFL+G+F+SSW+G++V +GEW IK+MP V+HIY+ASKQ+S AISPDQN AFK
Sbjct: 372 LTSLAFIFLVGIFVSSWVGSTVFWVGEWFIKKMPFVKHIYSASKQVSTAISPDQNTTAFK 551
Query: 542 EVVIIRHPRVGE 507
EV IIRHPRVGE
Sbjct: 552 EVAIIRHPRVGE 587
Score = 149 bits (320), Expect = 2e-035
Identities = 70/132 (53%), Positives = 84/132 (63%)
Frame = +1 / -3
Query: 508 SPTRGCLMMTTSLNACLF*SGDIAALICFDAL*I*RTRGMRLIIHSPRLRTDAPSQDDIK 687
SPTRGC ++ TSLNA +F*SG++A L CFDA + T G+ I HSP +T P+ ++
Sbjct: 586 SPTRGCRIIATSLNAVVF*SGEMAVLTCFDAEYMCFTNGIFFINHSPTQKTVEPTHEETN 407
Query: 688 TPIKNMKVTDVTKPRPNMLIPS*A*IGEKNPSTK*MNHQVM*NVIAMGKRITHPVMNFFE 867
P +NM DV KPRPNM P A G KNPST * NH VM NV A GKR THPV+NF +
Sbjct: 406 IPTRNMNARDVRKPRPNMSTPIFAYSGLKNPSTN*TNHHVMKNVKATGKRTTHPVINFLD 227
Query: 868 AQLRTTLWKASR 903
QL T A R
Sbjct: 226 TQL*RTAQHALR 191
Score = 113 bits (242), Expect = 1e-024
Identities = 57/132 (43%), Positives = 75/132 (56%)
Frame = +3 / -2
Query: 507 FSNTRMPYDDHFLERLFVLIW*YCSTNLLRCIVDMTNKRHALNNPLAKAKDRCPKPR*HK 686
F+NT M + + +LIW* S +LL C + + K H L P + +D P +K
Sbjct: 587 FTNTWMSNYRNLFKCCRILIW*NGSADLL*CRIYVLYKWHFLYKPFSNPEDS*THP*RNK 408
Query: 687 DSDQKYESNRCDKAKTEYVDSQLSIDWREESINKMNEPPGDVERYCYGEENYTSSHELL* 866
S+QKYE RC K KT+YVDS I W E+SINK+NEPP D ER ++N+TSSH+
Sbjct: 407 YSNQKYECQRCQKTKTKYVDSDFCI*WAEKSINKLNEPPCDEERESNRKKNHTSSHKFSR 228
Query: 867 SPTADNLMESLA 902
PT N LA
Sbjct: 227 YPTLKNSAACLA 192
>gb|BU997246.1|BU997246 HI07G08r HI Hordeum vulgare subsp. vulgare cDNA clone HI07G08
5-PRIME, mRNA sequence
Length = 640
Score = 245 bits (529), Expect = 3e-064
Identities = 90/135 (66%), Positives = 116/135 (85%)
Frame = -2 / +2
Query: 902 REAFHKVVRSWASKKFMTGCVILFPIAITFYITWWFIHFVDGFFSPIYAQLGINIFGLGF 723
R+A V++SW S+KFMTGCV++FP+A+TF+IT WFI F DGFFSP+YA+LG ++FGLGF
Sbjct: 236 RKACFAVLQSWVSRKFMTGCVVIFPMAVTFFITLWFIRFFDGFFSPLYAKLGFDVFGLGF 415
Query: 722 VTSVTFIFLIGVFMSSWLGASVLSLGEWIIKRMPLVRHIYNASKQISAAISPDQNKQAFK 543
VTS+ FIF++G+F+SSW+G++V +GEW IK+MP VRHIY+ASKQ+S A+SPDQN AFK
Sbjct: 416 VTSLVFIFIVGIFVSSWVGSTVFWVGEWFIKKMPFVRHIYSASKQVSTAVSPDQNTTAFK 595
Query: 542 EVVIIRHPRVGEYAF 498
EV II HPR GEYAF
Sbjct: 596 EVAIISHPRAGEYAF 640
Score = 146 bits (314), Expect = 1e-034
Identities = 71/135 (52%), Positives = 84/135 (62%)
Frame = +1 / -2
Query: 499 NAYSPTRGCLMMTTSLNACLF*SGDIAALICFDAL*I*RTRGMRLIIHSPRLRTDAPSQD 678
NAYSP RG L++ TSLNA +F*SG+ A L CFDA + T G+ I HSP +T P+ D
Sbjct: 639 NAYSPARGWLIIATSLNAVVF*SGETAVLTCFDAEYMCLTNGIFFINHSPTQKTVEPTHD 460
Query: 679 DIKTPIKNMKVTDVTKPRPNMLIPS*A*IGEKNPSTK*MNHQVM*NVIAMGKRITHPVMN 858
D P NM DVT PRPN PS A* G K PS +NH+VM NV A+GK THPV+N
Sbjct: 459 DTNIPTINMNTRDVTNPRPNTSNPSFA*SGLKKPSKNRINHKVMKNVTAIGKITTHPVIN 280
Query: 859 FFEAQLRTTLWKASR 903
F + QL T A R
Sbjct: 279 FLDTQL*RTAKHALR 235
Score = 112 bits (240), Expect = 2e-024
Identities = 51/125 (40%), Positives = 75/125 (60%)
Frame = +3 / -1
Query: 498 KRIFSNTRMPYDDHFLERLFVLIW*YCSTNLLRCIVDMTNKRHALNNPLAKAKDRCPKPR 677
K +F++T M HF + +LIW* S NLL C + + K H L P + +D P
Sbjct: 640 KCVFTSTGMANYCHFFKCCCILIW*NSSANLL*CRIYVPYKWHFLYKPFSNPEDS*AHP* 461
Query: 678 *HKDSDQKYESNRCDKAKTEYVDSQLSIDWREESINKMNEPPGDVERYCYGEENYTSSHE 857
*H+ S+ KYE RC K K +YV+S+L I+W E+++ + N+P D E + + E N+TSSH+
Sbjct: 460 *HEYSNNKYEYKRCHKPKAKYVESKLCIEWTEKTVEESNKPQSDEECHSHWENNHTSSHK 281
Query: 858 LL*SP 872
L * P
Sbjct: 280 LS*HP 266
>gb|BJ460841.1|BJ460841 BJ460841 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak42g23 3', mRNA sequence
Length = 644
Score = 231 bits (498), Expect = 6e-060
Identities = 90/122 (73%), Positives = 110/122 (90%)
Frame = -2 / -1
Query: 650 LGEWIIKRMPLVRHIYNASKQISAAISPDQNKQAFKEVVIIRHPRVGEYAFGFITSSVSL 471
+GEW IK+MP V+HIY+ASKQ+S AISPDQN AFKEV IIRHPRVGEYAFGFITS+V L
Sbjct: 644 VGEWFIKKMPFVKHIYSASKQVSTAISPDQNTTAFKEVAIIRHPRVGEYAFGFITSTVVL 465
Query: 470 QSYSGQEDLYCVYVPTNHLYIGDIFMVNSKDVIRPNLSVREGIEIVVSGGMSMPQILSTL 291
Q+ G E+L VYVPTNHLYIGDIF++NS+++IRPNLS+REGIEI+VSGGM+MPQ++++L
Sbjct: 464 QTDKGDEELCSVYVPTNHLYIGDIFLLNSEEIIRPNLSIREGIEIIVSGGMTMPQVIASL 285
Query: 290 DP 285
DP
Sbjct: 284 DP 279
Score = 143 bits (307), Expect = 1e-033
Identities = 66/113 (58%), Positives = 82/113 (72%)
Frame = +1 / +1
Query: 310 GIDIPPDTTISMPSRTERFGLMTSFEFTMKISPM*RWFVGT*TQ*RSS*PE*LWRDTDDV 489
G+ IPP+T IS+PSR ++FGLM S EF+ ISP+ R FVGT*T SS P +WR T DV
Sbjct: 304 GMVIPPETIISIPSRMDKFGLMISSEFSRNISPIYR*FVGT*TLHNSSSPLSVWRTTVDV 483
Query: 490 MKPNAYSPTRGCLMMTTSLNACLF*SGDIAALICFDAL*I*RTRGMRLIIHSP 648
+ PNAYSPTRGC ++ TSLNA +F*SG++A L CFDA + T G+ I HSP
Sbjct: 484 INPNAYSPTRGCRIIATSLNAVVF*SGEMAVLTCFDAEYMCFTNGIFFINHSP 642
>gb|CV061394.1|CV061394 BNEL68d1 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL68d1 5' similar to unknown
protein, mRNA sequence
Length = 554
Score = 226 bits (489), Expect = 1e-058
Identities = 83/127 (65%), Positives = 109/127 (85%)
Frame = -2 / +2
Query: 902 REAFHKVVRSWASKKFMTGCVILFPIAITFYITWWFIHFVDGFFSPIYAQLGINIFGLGF 723
R+A V++SW S+KFMTGCV++FP+A+TF+IT WFI F DGFFSP+YA+LG ++FGLGF
Sbjct: 173 RKACFAVLQSWVSRKFMTGCVVIFPMAVTFFITLWFIRFFDGFFSPLYAKLGFDVFGLGF 352
Query: 722 VTSVTFIFLIGVFMSSWLGASVLSLGEWIIKRMPLVRHIYNASKQISAAISPDQNKQAFK 543
VTS+ FIF++G+F+SSW+G++V +GEW IK+MP VRHIY+ASKQ+S A+SPDQN AFK
Sbjct: 353 VTSLVFIFIVGIFVSSWVGSTVFWVGEWFIKKMPFVRHIYSASKQVSTAVSPDQNTTAFK 532
Query: 542 EVVIIRH 522
EV II H
Sbjct: 533 EVAIISH 553
Score = 129 bits (277), Expect = 2e-029
Identities = 64/126 (50%), Positives = 77/126 (61%)
Frame = +1 / -3
Query: 526 LMMTTSLNACLF*SGDIAALICFDAL*I*RTRGMRLIIHSPRLRTDAPSQDDIKTPIKNM 705
L++ TSLNA +F*SG+ A L CFDA + T G+ I HSP +T P+ DD P NM
Sbjct: 549 LIIATSLNAVVF*SGETAVLTCFDAEYMCLTNGIFFINHSPTQKTVEPTHDDTNIPTINM 370
Query: 706 KVTDVTKPRPNMLIPS*A*IGEKNPSTK*MNHQVM*NVIAMGKRITHPVMNFFEAQLRTT 885
DVT PRPN PS A* G K PS +NH+VM NV A+GK THPV+NF + QL T
Sbjct: 369 NTRDVTNPRPNTSNPSFA*SGLKKPSKNRINHKVMKNVTAIGKITTHPVINFLDTQL*RT 190
Query: 886 LWKASR 903
A R
Sbjct: 189 AKHALR 172
Score = 109 bits (233), Expect = 2e-023
Identities = 48/112 (42%), Positives = 68/112 (60%)
Frame = +3 / -2
Query: 537 HFLERLFVLIW*YCSTNLLRCIVDMTNKRHALNNPLAKAKDRCPKPR*HKDSDQKYESNR 716
HF + +LIW* S NLL C + + K H L P + +D P *H+ S+ KYE R
Sbjct: 538 HFFKCCCILIW*NSSANLL*CRIYVPYKWHFLYKPFSNPEDS*AHP**HEYSNNKYEYKR 359
Query: 717 CDKAKTEYVDSQLSIDWREESINKMNEPPGDVERYCYGEENYTSSHELL*SP 872
C K K +YV+S+L I+W E+++ + N+P D E + + E NYTSSH+L * P
Sbjct: 358 CHKPKAKYVESKLCIEWTEKTVEESNKPQSDEECHSHWENNYTSSHKLS*HP 203
>gb|CV064122.1|CV064122 BNEL98c5 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL98c5 5' similar to Oryza sativa,
mRNA sequence
Length = 648
Score = 226 bits (489), Expect = 1e-058
Identities = 85/140 (60%), Positives = 111/140 (79%)
Frame = -2 / +2
Query: 914 GRVDREAFHKVVRSWASKKFMTGCVILFPIAITFYITWWFIHFVDGFFSPIYAQLGINIF 735
G R+A V++SW +KFMTGCV++FP+A+TF+IT WFI F DGFFSP+YA+LG ++F
Sbjct: 161 GTSTRKACFAVLQSWVPRKFMTGCVVIFPMAVTFFITLWFIRFFDGFFSPLYAKLGFDVF 340
Query: 734 GLGFVTSVTFIFLIGVFMSSWLGASVLSLGEWIIKRMPLVRHIYNASKQISAAISPDQNK 555
GLG+ TS+ FI + G+F SSW+G++ +GEW IK+MP VRHIY+ASKQ+S +SPDQN
Sbjct: 341 GLGYGTSLVFILIRGIFESSWVGSTDFWVGEWFIKKMPFVRHIYSASKQVSTDVSPDQNT 520
Query: 554 QAFKEVVIIRHPRVGEYAFG 495
A+KEV IIRH R GEYAFG
Sbjct: 521 TAYKEVAIIRHTRAGEYAFG 580
Score = 141 bits (303), Expect(2) = 4e-035
Identities = 70/140 (50%), Positives = 84/140 (60%)
Frame = +1 / -1
Query: 496 PNAYSPTRGCLMMTTSLNACLF*SGDIAALICFDAL*I*RTRGMRLIIHSPRLRTDAPSQ 675
PNAYSP R CL+M TSL A +F*SG+ + L CFDA + T G+ I HSP ++ P+
Sbjct: 579 PNAYSPARVCLIMATSLYAVVF*SGETSVLTCFDAEYMCLTNGIFFINHSPTQKSVEPTH 400
Query: 676 DDIKTPIKNMKVTDVTKPRPNMLIPS*A*IGEKNPSTK*MNHQVM*NVIAMGKRITHPVM 855
DD P+ NM DV PRPN PS A* G K PS +NH+VM NV A+GK THPV+
Sbjct: 399 DDSNIPLINMNTRDVPYPRPNTSNPSFA*SGLKKPSKNRINHKVMKNVTAIGKITTHPVI 220
Query: 856 NFFEAQLRTTLWKASRSTRP 915
NF QL T A R P
Sbjct: 219 NFLGTQL*RTAKHALRVEVP 160
Score = 102 bits (218), Expect = 2e-021
Identities = 47/122 (38%), Positives = 71/122 (58%)
Frame = +3 / -3
Query: 495 TKRIFSNTRMPYDDHFLERLFVLIW*YCSTNLLRCIVDMTNKRHALNNPLAKAKDRCPKP 674
+K +F++T MP HF R +LIW* S NLL C + + K H L P + + P
Sbjct: 580 SKCVFTSTGMPNYGHFFIRCCILIW*NISANLL*CRIYVPYKWHFLYKPFSNPEVS*AHP 401
Query: 675 R*HKDSDQKYESNRCDKAKTEYVDSQLSIDWREESINKMNEPPGDVERYCYGEENYTSSH 854
* + S +YE RC + +YV+S+L I+W E+++ + N+P D E + + E NYTSSH
Sbjct: 400 **LEYSPNQYEYERCPVPQAKYVESKLCIEWTEKTVEESNKPQSDEECHSHWENNYTSSH 221
Query: 855 EL 860
+L
Sbjct: 220 KL 215
Score = 26.7 bits (52), Expect(2) = 4e-035
Identities = 8/21 (38%), Positives = 16/21 (76%)
Frame = +3 / -1
Query: 426 WDIDAVKIFLTRITLEGY*RC 488
W IDA ++F+T ++++ + RC
Sbjct: 648 WLIDATQLFITFVSVKNHARC 586
>gb|CA014678.1|CA014678 HT12A16r HT Hordeum vulgare subsp. vulgare cDNA clone HT12A16
5-PRIME, mRNA sequence
Length = 675
Score = 207 bits (446), Expect = 9e-053
Identities = 72/106 (67%), Positives = 97/106 (91%)
Frame = -2 / +3
Query: 884 VVRSWASKKFMTGCVILFPIAITFYITWWFIHFVDGFFSPIYAQLGINIFGLGFVTSVTF 705
V++SW S+KFMTGCV+LFP+A TF+ITWWF+ FVDGFFSP+YA++G++IFGLGF+TS+ F
Sbjct: 18 VLQSWVSRKFMTGCVVLFPVAFTFFITWWFVQFVDGFFSPLYAKIGVDIFGLGFLTSLAF 197
Query: 704 IFLIGVFMSSWLGASVLSLGEWIIKRMPLVRHIYNASKQISAAISP 567
IFL+G+F+SSW+G++V +GEW IK+MP V+HIY+ASKQ+S AISP
Sbjct: 198 IFLVGIFVSSWVGSTVFWVGEWFIKKMPFVKHIYSASKQVSTAISP 335
Score = 116 bits (247), Expect = 2e-025
Identities = 53/103 (51%), Positives = 64/103 (62%)
Frame = +1 / -3
Query: 568 GDIAALICFDAL*I*RTRGMRLIIHSPRLRTDAPSQDDIKTPIKNMKVTDVTKPRPNMLI 747
G++A L CFDA + T G+ I HSP +T P+ ++ P +NM DV KPRPNM
Sbjct: 334 GEMAVLTCFDAEYMCFTNGIFFINHSPTQKTVEPTHEETNIPTRNMNARDVKKPRPNMST 155
Query: 748 PS*A*IGEKNPSTK*MNHQVM*NVIAMGKRITHPVMNFFEAQL 876
P A G KNPST * NH VM NV A GKR THPV+NF + QL
Sbjct: 154 PIFAYSGLKNPSTN*TNHHVMKNVKATGKRTTHPVINFLDTQL 26
Score = 104 bits (221), Expect = 9e-022
Identities = 50/118 (42%), Positives = 67/118 (56%)
Frame = +3 / -2
Query: 531 DDHFLERLFVLIW*YCSTNLLRCIVDMTNKRHALNNPLAKAKDRCPKPR*HKDSDQKYES 710
DD + + W* S +LL C + + + H L P + +D P +K S+QKYE
Sbjct: 371 DDQASHMIRRITW*NGSADLL*CRIYVLYEWHFLYKPFSNPEDS*THP*RNKYSNQKYEC 192
Query: 711 NRCDKAKTEYVDSQLSIDWREESINKMNEPPGDVERYCYGEENYTSSHELL*SPTADN 884
RC K KT+YVDS I W E+SINK+NEPP D ER ++N+TSSH+ PT N
Sbjct: 191 QRCQKTKTKYVDSDFCI*WAEKSINKLNEPPCDEERESNRKKNHTSSHKFSRYPTLKN 18
>emb|AJ234443.1|HVU234443 Hordeum vulgare partial mRNA; clone cMWG0733.rev
Length = 239
Score = 185 bits (398), Expect = 4e-046
Identities = 71/79 (89%), Positives = 75/79 (94%)
Frame = -2 / +1
Query: 854 MTGCVILFPIAITFYITWWFIHFVDGFFSPIYAQLGINIFGLGFVTSVTFIFLIGVFMSS 675
MTGCVILFPIAITFY TWWFIHFVDGFFSPIYAQLG NIFGLGF+TSVTFIF +GVFMSS
Sbjct: 1 MTGCVILFPIAITFYFTWWFIHFVDGFFSPIYAQLGXNIFGLGFITSVTFIFFVGVFMSS 180
Query: 674 WLGASVLSLGEWIIKRMPL 618
W+GASVL +GEWIIKRMPL
Sbjct: 181 WVGASVLGIGEWIIKRMPL 237
Score = 151 bits (325), Expect = 4e-036
Identities = 65/79 (82%), Positives = 70/79 (88%)
Frame = +1 / -1
Query: 616 TRGMRLIIHSPRLRTDAPSQDDIKTPIKNMKVTDVTKPRPNMLIPS*A*IGEKNPSTK*M 795
T GMRL+IHSP RTDAP+QDD+KTP KNMKVT+V KPRP M IP+ A*IGEKNPSTK*M
Sbjct: 239 TSGMRLMIHSPMPRTDAPTQDDMKTPTKNMKVTEVMKPRPKMXIPNCA*IGEKNPSTK*M 60
Query: 796 NHQVM*NVIAMGKRITHPV 852
NHQV *NVIAMGKRITHPV
Sbjct: 59 NHQVK*NVIAMGKRITHPV 3
Score = 143 bits (306), Expect = 2e-033
Identities = 57/79 (72%), Positives = 66/79 (83%)
Frame = +3 / -3
Query: 618 KRHALNNPLAKAKDRCPKPR*HKDSDQKYESNRCDKAKTEYVDSQLSIDWREESINKMNE 797
K HALN+P AK+RC PR*H+DS++KYESNR D+AKT+ V SQL IDWREESINKMNE
Sbjct: 237 KWHALNDPFTNAKNRCSNPR*HEDSNEKYESNRSDEAKTKNVXSQLCIDWREESINKMNE 58
Query: 798 PPGDVERYCYGEENYTSSH 854
PPG+VE Y YGEEN+ SSH
Sbjct: 57 PPGEVECYRYGEENHASSH 1
Score = 103 bits (219), Expect = 2e-021
Identities = 39/58 (67%), Positives = 44/58 (75%)
Frame = -1 / +3
Query: 789 FC*WILLSNLCSTGNQHIRSWLCHICYFHIFDRSLYVILAWGICP*PWRVDY*AHASC 616
FC*WILLSNLC+ GN H SWL H CYFHIF SL+VIL W IC W +D+*AHA+C
Sbjct: 66 FC*WILLSNLCTIGNXHFWSWLHHFCYFHIFRWSLHVILGWSICSWHW*MDH*AHATC 239
>gb|BJ447422.1|BJ447422 BJ447422 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak35h23 5', mRNA sequence
Length = 260
Score = 165 bits (355), Expect(2) = 9e-044
Identities = 67/73 (91%), Positives = 71/73 (97%)
Frame = -2 / +2
Query: 785 VDGFFSPIYAQLGINIFGLGFVTSVTFIFLIGVFMSSWLGASVLSLGEWIIKRMPLVRHI 606
VDGFFSPIYAQLGINIFGLGF+TSVTFIF +GVFMSSW+GASVL +GEWIIKRMPLVRHI
Sbjct: 41 VDGFFSPIYAQLGINIFGLGFITSVTFIFFVGVFMSSWVGASVLGIGEWIIKRMPLVRHI 220
Query: 605 YNASKQISAAISP 567
YNASKQISAAISP
Sbjct: 221 YNASKQISAAISP 259
Score = 135 bits (290), Expect(2) = 2e-032
Identities = 59/73 (80%), Positives = 64/73 (87%)
Frame = +1 / -3
Query: 568 GDIAALICFDAL*I*RTRGMRLIIHSPRLRTDAPSQDDIKTPIKNMKVTDVTKPRPNMLI 747
GDIAALIC DAL*I RT GMRL+IHSP RTDAP+QDD+KTP KNMKVT+V KPRP MLI
Sbjct: 258 GDIAALICLDAL*IWRTSGMRLMIHSPMPRTDAPTQDDMKTPTKNMKVTEVMKPRPKMLI 79
Query: 748 PS*A*IGEKNPST 786
P+ A*IGEKNPST
Sbjct: 78 PNCA*IGEKNPST 40
Score = 134 bits (288), Expect(2) = 1e-031
Identities = 50/74 (67%), Positives = 59/74 (79%)
Frame = -1 / +1
Query: 789 FC*WILLSNLCSTGNQHIRSWLCHICYFHIFDRSLYVILAWGICP*PWRVDY*AHASCSS 610
+C*WILLSNLC+ GNQH SWL H CYFHIF SL+VIL W IC W +D+*AHA+C+
Sbjct: 37 YC*WILLSNLCTIGNQHFWSWLHHFCYFHIFRWSLHVILGWSICSWHW*MDH*AHATCAP 216
Query: 609 YLQCIEAN*CCNIT 568
YLQC++AN*CC IT
Sbjct: 217 YLQCVQAN*CCYIT 258
Score = 126 bits (269), Expect = 2e-028
Identities = 52/73 (71%), Positives = 60/73 (82%)
Frame = +3 / -2
Query: 567 W*YCSTNLLRCIVDMTNKRHALNNPLAKAKDRCPKPR*HKDSDQKYESNRCDKAKTEYVD 746
W*Y STNLL IVDM +K HALN+P AK+RC PR*H+DS++KYESNR D+AKT+ VD
Sbjct: 259 W*YSSTNLLGRIVDMAHKWHALNDPFTNAKNRCSNPR*HEDSNEKYESNRSDEAKTKNVD 80
Query: 747 SQLSIDWREESIN 785
SQL IDWREESIN
Sbjct: 79 SQLCIDWREESIN 41
Score = 103 bits (220), Expect = 1e-021
Identities = 53/74 (71%), Positives = 56/74 (75%)
Frame = +2 / -1
Query: 566 LVILQH*FASMHCRYDEQEACA**STRQG*GQMPQAKMT*RLRSKI*K*QM*QSQDRIC* 745
LVI QH*FA HCRY Q ACA**S Q QM Q KMT*RL+ KI*K*Q * SQD+ C*
Sbjct: 260 LVI*QH*FAWTHCRYGAQVACA**SIHQCQEQMLQPKMT*RLQRKI*K*QK**SQDQKC* 81
Query: 746 FPVEHRLERRIHQQ 787
FP+ HRLERRIHQQ
Sbjct: 80 FPIVHRLERRIHQQ 39
Score = 31.8 bits (63), Expect(2) = 9e-044
Identities = 8/10 (80%), Positives = 10/10 (100%)
Frame = -2 / +1
Query: 815 FYITWWFIHF 786
FY+TWWFIH+
Sbjct: 10 FYLTWWFIHY 39
Score = 23.5 bits (45), Expect(2) = 2e-032
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = +3 / -3
Query: 789 MNEPPGDVE 815
MNEPPG VE
Sbjct: 36 MNEPPGQVE 10
Score = 21.7 bits (41), Expect(2) = 1e-031
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = -3 / +2
Query: 814 STSPGGSFILL 782
ST PGGSFI++
Sbjct: 11 ST*PGGSFIIV 43
>gb|CB858531.1|CB858531 HI07G08w HI Hordeum vulgare subsp. vulgare cDNA clone HI07G08
3-PRIME, mRNA sequence
Length = 580
Score = 131 bits (281), Expect = 5e-030
Identities = 52/73 (71%), Positives = 64/73 (87%)
Frame = -2 / -3
Query: 503 AFGFITSSVSLQSYSGQEDLYCVYVPTNHLYIGDIFMVNSKDVIRPNLSVREGIEIVVSG 324
AFGFITSS+ LQ+ G E+L VYVPTNHLYIGDIF+VNS ++IRPNLS+REGIEI+VSG
Sbjct: 578 AFGFITSSMILQTDKGDEELCSVYVPTNHLYIGDIFLVNSAEIIRPNLSIREGIEIIVSG 399
Query: 323 GMSMPQILSTLDP 285
GM+MPQ+++ L P
Sbjct: 398 GMTMPQVITALGP 360
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 431,662,679
Number of Sequences: 312970
Number of extensions: 6592042
Number of successful extensions: 37281
Number of sequences better than 1.0e-020: 20
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 10736453445
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)