TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3067043.2.1
(834 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG309831.1|BG309831 HVSMEc0013A09f Hordeum vulgare seedl... 201 2e-073
gb|BF623091.2|BF623091 HVSMEa0014D14f Hordeum vulgare seedl... 162 3e-039
gb|AV912784.1|AV912784 AV912784 K. Sato unpublished cDNA li... 155 4e-039
gb|AV915960.1|AV915960 AV915960 K. Sato unpublished cDNA li... 154 6e-037
gb|BE060120.2|BE060120 HVSMEg0011I12f Hordeum vulgare pre-a... 116 2e-025
gb|BG415849.1|BG415849 HVSMEk0008C14f Hordeum vulgare testa... 109 3e-023
gb|BF260956.1|BF260956 HVSMEf0023G04f Hordeum vulgare seedl... 105 4e-022
gb|AV917519.1|AV917519 AV917519 K. Sato unpublished cDNA li... 71 1e-021
>gb|BG309831.1|BG309831 HVSMEc0013A09f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0013A09f, mRNA sequence
Length = 792
Score = 201 bits (434), Expect(3) = 2e-073
Identities = 83/125 (66%), Positives = 96/125 (76%)
Frame = +3 / +2
Query: 90 FPTKPSPKVLHPFASLVTVNDAIWKMYFDELLPLFTVDGDDGSYAQTVALDLACLQVLSQ 269
FPTK PKVLHPFAS V VNDAIWKMYF+ELLPLFT DGDDG+YA+TVALD ACLQ LS+
Sbjct: 290 FPTKGYPKVLHPFASSVCVNDAIWKMYFNELLPLFTADGDDGNYAETVALDFACLQALSR 469
Query: 270 RIHIGKYVAEVKFKDAPQEYSRLIKEKDSNSLMDMLTFXXXXXXXXXXXXXXXXTFGQNV 449
RIH GKYVAEVKF+DAP +YS I+ KD+N+LM++LTF FGQNV
Sbjct: 470 RIHCGKYVAEVKFRDAPHDYSPPIRAKDNNALMNLLTFTGVEEKVKNRVEKTATVFGQNV 649
Query: 450 TLDDN 464
TL+D+
Sbjct: 650 TLEDS 664
Score = 175 bits (377), Expect(3) = 2e-055
Identities = 83/124 (66%), Positives = 90/124 (72%)
Frame = -3 / -1
Query: 463 LSSKVTFCPNVXXXXXXXXXXXXXXXLNVSISIRELLSFSFISRLYS*GASLNFTSATYL 284
LSS+VTFCPN +NVS IR LLS + I L S*GASLNFTSATYL
Sbjct: 663 LSSRVTFCPNTVAVFSTLFLTFSSTPVNVSKFIRALLSLARIGGL*S*GASLNFTSATYL 484
Query: 283 PI*ILCDRTCRHAKSSATVCA*LPSSPSTVNNGSNSSKYIFHIASFTVTNEAKGCKTFGE 104
P *IL +R CRHAKS+ATV A*LPSSPS VN+GSNS KYIFHIASFT T+EAKGCKTFG
Sbjct: 483 PQ*ILLERACRHAKSNATVSA*LPSSPSAVNSGSNSLKYIFHIASFTQTDEAKGCKTFG* 304
Query: 103 GFVG 92
VG
Sbjct: 303 PLVG 292
Score = 149 bits (321), Expect(3) = 9e-046
Identities = 63/98 (64%), Positives = 73/98 (74%)
Frame = +2 / +1
Query: 89 LSYKAFPKGLAPFCFIGHRE*CNMENVF**IAPVIHC*W**WQLCTNSCTGFSVSAGSIT 268
+SY+ PKG APFCFI E*CNME+VF *IA IHC W *WQLC NSC F +SAGS+
Sbjct: 289 VSYQRLPKGFAPFCFISLCE*CNMEDVFQ*IATTIHCGWR*WQLCRNSCIRFCMSAGSLK 468
Query: 269 KNSYW*ICC*GEVQRRSSRI*STNKRKGQQFSDGYADI 382
KNS W +CC*GE+QRR+SR+* TN R GQQ SD +I
Sbjct: 469 KNSLWQVCC*GEIQRRASRL*PTNTR*GQQCSDELTNI 582
Score = 134 bits (287), Expect(3) = 3e-041
Identities = 62/99 (62%), Positives = 68/99 (68%)
Frame = -1 / -2
Query: 387 P*MSAYPSENCCPFLLLVDYILEERL*TSPQQHIYQYEFFVIEPADTLNPVQLFVHSCHH 208
P*M SE+CCP +LV Y E RL* SPQQH EFF+ EPAD N +QLF+HSCHH
Sbjct: 587 P*MLVSSSEHCCP*RVLVGYNREARL*ISPQQHTCHNEFFLREPADMQNLMQLFLHSCHH 408
Query: 207 HHQQ*ITGAIHQNTFSILHHSR*PMKQKGARPLGKAL*E 91
H Q*I AIH NT SILHHS MKQKGA+PLG *E
Sbjct: 407 RHPQ*IVVAIH*NTSSILHHSHRLMKQKGAKPLGNLW*E 291
Score = 119 bits (255), Expect = 2e-026
Identities = 59/98 (60%), Positives = 64/98 (65%)
Frame = -2 / -3
Query: 383 ECQHIHQRIAVLFFY*STIFLRSVFELHLSNIFTNMNSL**NLQTR*IQCNCLCIAAIIT 204
EC +HQ I VL Y I +R V E HLSNI MNS * +LQT I CNC CI AII
Sbjct: 583 EC**VHQSIVVLSAYWWAIIVRRVSEFHLSNILATMNSS*ESLQTCKI*CNCFCIVAIIA 404
Query: 203 INSE*REQFIKIHFPYCIIHGDQ*SKRVQDLWGRLCRK 90
I SE* QFI+IH PYCIIH D *SKRVQ+LW RK
Sbjct: 403 IRSE*W*QFIEIHLPYCIIHTD**SKRVQNLWVTFGRK 290
Score = 113 bits (242), Expect = 1e-024
Identities = 57/98 (58%), Positives = 65/98 (66%)
Frame = +1 / +3
Query: 91 FLQSLPQRSCTLLLHWSP*MMQYGKCILMNCSRYSLLMVMMAAMHKQLHWI*RVCRFYHK 270
FL + QR CTLLLH S *MMQYG+CI MNC YSL M MMA M KQLH I VCR +
Sbjct: 291 FLPKVTQRFCTLLLHQSV*MMQYGRCISMNCYHYSLRMAMMATMQKQLH*ILHVCRLSQE 470
Query: 271 EFILVNMLLR*SSKTLLKNIVD**KKRTAIL*WIC*HS 384
EFI+ +MLLR*+S+T L I RT +L* *HS
Sbjct: 471 EFIVASMLLR*NSETRLTIIAHQYALRTTML**TY*HS 584
Score = 62.9 bits (131), Expect(3) = 2e-073
Identities = 25/33 (75%), Positives = 26/33 (78%)
Frame = +3 / +2
Query: 477 DSECKVDPKVLSKLYDQWVMPLTKDVEVEYLLR 575
DS C DPKVLSKLYD WVMP TKDVE E+L R
Sbjct: 692 DSHCIGDPKVLSKLYDIWVMPFTKDVETEFLFR 790
Score = 51.0 bits (105), Expect(3) = 2e-073
Identities = 22/31 (70%), Positives = 23/31 (74%)
Frame = +1 / +2
Query: 1 GKQRPSTQRLDIIKSQKMFHSSLKIYPHLSF 93
GK R T RLD IKSQKMFHS LKI+ HL F
Sbjct: 200 GKPRSCTPRLDTIKSQKMFHSCLKIFHHLWF 292
Score = 48.3 bits (99), Expect(3) = 9e-046
Identities = 21/28 (75%), Positives = 23/28 (82%)
Frame = +2 / +3
Query: 2 GSRGPQRKGXTLSKARRCSILPSRSTLT 85
GS GP R+G TLSKARRCSIL SRS+ T
Sbjct: 201 GSPGPARQGWTLSKARRCSILVSRSSTT 284
Score = 45.1 bits (92), Expect(3) = 2e-055
Identities = 18/24 (75%), Positives = 21/24 (87%)
Frame = -2 / -1
Query: 74 ILREEWNIFWLLIMSSLCVEGLCF 3
ILR+EWNIFWLLI+SSL V+ L F
Sbjct: 273 ILRQEWNIFWLLIVSSLGVQDLGF 202
Score = 44.6 bits (91), Expect(3) = 3e-041
Identities = 20/29 (68%), Positives = 22/29 (75%)
Frame = -1 / -3
Query: 87 EVRVDLEGRMEHLLAFDNVXPLR*GPLLP 1
+V DLE RMEHLLAFD+V P R GP LP
Sbjct: 286 QVVEDLETRMEHLLAFDSVQPWRAGPGLP 200
Score = 34.1 bits (68), Expect(3) = 2e-055
Identities = 15/23 (65%), Positives = 17/23 (73%)
Frame = -3 / -1
Query: 544 VSGITH*SYSLESTLGSTLHSLS 476
V GITH SYSL+ST GS + LS
Sbjct: 759 VKGITHISYSLDSTFGSPMQ*LS 691
Score = 28.1 bits (55), Expect(3) = 3e-041
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = -2 / -3
Query: 557 FDILGQWHHPLIIQLGEHFGIDLALAV 477
F+I + H+P II L +HF I A+ V
Sbjct: 772 FNIFRKGHYPYII*LRQHFWITYAVTV 692
Score = 24.0 bits (46), Expect(3) = 9e-046
Identities = 14/32 (43%), Positives = 17/32 (53%)
Frame = +1 / +3
Query: 463 MPLLVTASARSIPKCSPSCMISG*CH*PRMSK 558
M + T +A I KC S MI G*C +M K
Sbjct: 678 MVIPATVTA*VIQKCCLSYMIYG*CPLRKMLK 773
>gb|BF623091.2|BF623091 HVSMEa0014D14f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0014D14f, mRNA sequence
Length = 903
Score = 162 bits (348), Expect = 3e-039
Identities = 66/111 (59%), Positives = 79/111 (71%)
Frame = +3 / +2
Query: 3 EAEALNAKAXHYQKPEDVPFFPQDLPSPLFPTKPSPKVLHPFASLVTVNDAIWKMYFDEL 182
EA+ L+AKA HYQKPEDVPF DLP P+FPTK PKVLHP A+ NDA WKMY +EL
Sbjct: 176 EAQVLHAKAGHYQKPEDVPFLSHDLPPPVFPTKGYPKVLHPSATSACENDAFWKMYLNEL 355
Query: 183 LPLFTVDGDDGSYAQTVALDLACLQVLSQRIHIGKYVAEVKFKDAPQEYSR 335
LPLFT D +G+YA+T ALD AC+++LS+ + K VKF DAP E R
Sbjct: 356 LPLFTADWHEGNYAKTAALDFACMKLLSREFIVVKMPLRVKFNDAPHENYR 508
Score = 116 bits (248), Expect = 2e-025
Identities = 57/106 (53%), Positives = 69/106 (65%)
Frame = +2 / +1
Query: 2 GSRGPQRKGXTLSKARRCSILPSRSTLTSLSYKAFPKGLAPFCFIGHRE*CNMENVF**I 181
GS GP R+G TLSKARRCSIL SRS+ T LSY+ PKG APFC+I E*C +E+V *I
Sbjct: 175 GSPGPARQGWTLSKARRCSILVSRSSTTCLSYQRLPKGFAPFCYISL*E*CILEDVSQ*I 354
Query: 182 APVIHC*W**WQLCTNSCTGFSVSAGSITKNSYW*ICC*GEVQRRS 319
A +HC * +LC NSC F + S+ + *GE+QRRS
Sbjct: 355 ATTLHCGLA*RKLCKNSCIRFCMYEASLKRIHCGKDAA*GEIQRRS 492
Score = 111 bits (237), Expect(3) = 3e-026
Identities = 54/88 (61%), Positives = 62/88 (70%)
Frame = -3 / -1
Query: 265 DRTCRHAKSSATVCA*LPSSPSTVNNGSNSSKYIFHIASFTVTNEAKGCKTFGEGFVGKR 86
+R+ HAKS+A V A* PS S V +GSNS +YIF ASF+ + A+GCKTFG VGK
Sbjct: 438 ERSFIHAKSNAAVFA*FPSCQSAVKSGSNSLRYIFQNASFSQADVAEGCKTFG*PLVGKT 259
Query: 85 GEGRS*GKNGTSSGF**CXAFALRASAS 2
G GRS* KNGTSSGF**C A A R AS
Sbjct: 258 GGGRS*DKNGTSSGF**CPALACRTWAS 175
Score = 109 bits (232), Expect = 3e-023
Identities = 57/112 (50%), Positives = 64/112 (57%)
Frame = +1 / +3
Query: 1 GKQRPSTQRLDIIKSQKMFHSSLKIYPHLSFLQSLPQRSCTLLLHWSP*MMQYGKCILMN 180
GK R T RLD IKSQKMFHS L I+ HLSFL + QR CTLLLH MM G+CI MN
Sbjct: 174 GKPRSCTPRLDTIKSQKMFHSCLTIFHHLSFLPKVTQRFCTLLLHQPVRMMHSGRCISMN 353
Query: 181 CSRYSLLMVMMAAMHKQLHWI*RVCRFYHKEFILVNMLLR*SSKTLLKNIVD 336
C SL + M M KQLH I V F + + L *+S TL I D
Sbjct: 354 CYHSSLRIGMKETMQKQLH*ILHV*SFSQENSLW*RCRLG*NSTTLPMRITD 509
Score = 22.6 bits (43), Expect(3) = 3e-026
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = -3 / -1
Query: 370 SIRELLSFSFISRLYS*GASLNFT 299
S+ +S + R +S GASLNFT
Sbjct: 543 SLHSFVSLAR*CR*FSWGASLNFT 472
Score = 22.1 bits (42), Expect(3) = 3e-026
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = -2 / -1
Query: 299 LSNIFTNMNSL 267
LS IFT MNSL
Sbjct: 471 LSGIFTTMNSL 439
>gb|AV912784.1|AV912784 AV912784 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags10e01 5', mRNA sequence
Length = 543
Score = 155 bits (333), Expect(2) = 4e-039
Identities = 58/123 (47%), Positives = 81/123 (65%)
Frame = +3 / +3
Query: 15 LNAKAXHYQKPEDVPFFPQDLPSPLFPTKPSPKVLHPFASLVTVNDAIWKMYFDELLPLF 194
L+AK Y+ P++ PFFP+DLP L P P VLHP A + +N IWKMYFDE++P
Sbjct: 9 LHAKVGRYKSPDEHPFFPEDLPETLLPPIQYPTVLHPIADSININKEIWKMYFDEVIPRL 188
Query: 195 TVDGDDGSYAQTVALDLACLQVLSQRIHIGKYVAEVKFKDAPQEYSRLIKEKDSNSLMDM 374
+G DG+ + D CLQ LS+RIH GK+VAE KF+++P+ Y+ IK +D + LM +
Sbjct: 189 VKEGSDGNSGSSALCDTTCLQALSKRIHYGKFVAEAKFQESPEAYTPAIKAQDGDQLMQL 368
Query: 375 LTF 383
LT+
Sbjct: 369 LTY 377
Score = 26.3 bits (51), Expect(2) = 4e-039
Identities = 9/20 (45%), Positives = 14/20 (70%)
Frame = +3 / +2
Query: 489 KVDPKVLSKLYDQWVMPLTK 548
K+ P +++ LY +MPLTK
Sbjct: 482 KIRPSLVAGLYSNRIMPLTK 541
>gb|AV915960.1|AV915960 AV915960 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags16n12 5', mRNA sequence
Length = 470
Score = 154 bits (331), Expect = 6e-037
Identities = 60/144 (41%), Positives = 83/144 (57%)
Frame = +3 / +3
Query: 36 YQKPEDVPFFPQDLPSPLFPTKPSPKVLHPFASLVTVNDAIWKMYFDELLPLFTVDGDDG 215
Y+ P++ PFFP+DLP L P P VLHP A + +N IWKMYFDE++P +G DG
Sbjct: 12 YKSPDEHPFFPEDLPETLLPPIQYPTVLHPIADSININKEIWKMYFDEVIPRLVKEGSDG 191
Query: 216 SYAQTVALDLACLQVLSQRIHIGKYVAEVKFKDAPQEYSRLIKEKDSNSLMDMLTFXXXX 395
+ + D CLQ LS+RIH GK+VAE KF+++P+ Y+ IK +D + LM +LT+
Sbjct: 192 NSGSSALCDTTCLQALSKRIHYGKFVAEAKFQESPEAYTPAIKAQDGDQLMQLLTYETVE 371
Query: 396 XXXXXXXXXXXXTFGQNVTLDDNA 467
FGQ V + A
Sbjct: 372 RAIEHRVETKAKIFGQEVNIGAEA 443
>gb|BE060120.2|BE060120 HVSMEg0011I12f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0011I12f, mRNA sequence
Length = 592
Score = 116 bits (248), Expect = 2e-025
Identities = 46/110 (41%), Positives = 65/110 (59%)
Frame = +3 / +3
Query: 3 EAEALNAKAXHYQKPEDVPFFPQDLPSPLFPTKPSPKVLHPFASLVTVNDAIWKMYFDEL 182
E E L+AK Y+ P++ PFFP+DLP L P P VLHP A + +N IWKMYFDE+
Sbjct: 171 ETEKLHAKVGRYKSPDEHPFFPEDLPETLLPPIQYPTVLHPIADSININKEIWKMYFDEV 350
Query: 183 LPLFTVDGDDGSYAQTVALDLACLQVLSQRIHIGKYVAEVKFKDAPQEYS 332
+ + DG+ + D CLQ L +R + K+VA KF++ P+ Y+
Sbjct: 351 ISRLVKERSDGNS*FSALCDTTCLQALFKRNPLWKFVAXAKFQEVPEAYT 500
>gb|BG415849.1|BG415849 HVSMEk0008C14f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0008C14f, mRNA sequence
Length = 660
Score = 109 bits (232), Expect = 3e-023
Identities = 46/126 (36%), Positives = 67/126 (53%)
Frame = +3 / +1
Query: 3 EAEALNAKAXHYQKPEDVPFFPQDLPSPLFPTKPSPKVLHPFASLVTVNDAIWKMYFDEL 182
E E L+ + Y+ P + PFFP+DLP L+P VLHP A + +N IWKM +DE+
Sbjct: 190 EPETLHPRVGRYKSPYEHPFFPEDLPDTLWPPIQHTAVLHPIADSLNINRQIWKMSYDEV 369
Query: 183 LPLFTVDGDDGSYAQTVALDLACLQVLSQRIHIGKYVAEVKFKDAPQEYSRLIKEKDSNS 362
+P +G +G+ + D CLQVL + I G A+ + P Y+ IK +D
Sbjct: 370 IPRLAKEGSEGNGGSSALCDTTCLQVLYKTIPYGTSAAQANS*EPPDAYTPGIKPEDGEQ 549
Query: 363 LMDMLT 380
LM + T
Sbjct: 550 LMQLPT 567
>gb|BF260956.1|BF260956 HVSMEf0023G04f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0023G04f, mRNA sequence
Length = 746
Score = 105 bits (223), Expect = 4e-022
Identities = 39/85 (45%), Positives = 51/85 (60%)
Frame = +3 / +1
Query: 3 EAEALNAKAXHYQKPEDVPFFPQDLPSPLFPTKPSPKVLHPFASLVTVNDAIWKMYFDEL 182
E E L+AK Y+ P++ PFFP+DLP L P P VLHP A + +N IWKMYFDE+
Sbjct: 487 ETEKLHAKVGRYKSPDEHPFFPEDLPETLLPPIQYPTVLHPIADSININKEIWKMYFDEV 666
Query: 183 LPLFTVDGDDGSYAQTVALDLACLQ 257
+P +G DG+ + D CLQ
Sbjct: 667 IPRLVKEGSDGNSGSSALCDTTCLQ 741
>gb|AV917519.1|AV917519 AV917519 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags10e01 3', mRNA sequence
Length = 706
Score = 70.7 bits (148), Expect(2) = 1e-021
Identities = 29/73 (39%), Positives = 41/73 (56%)
Frame = +3 / -2
Query: 249 CLQVLSQRIHIGKYVAEVKFKDAPQEYSRLIKEKDSNSLMDMLTFXXXXXXXXXXXXXXX 428
CLQ LS+RIH GK+VAE KF+++P+ Y+ IK +D + LM +LT+
Sbjct: 678 CLQALSKRIHYGKFVAEAKFQESPEAYTPAIKAQDGDQLMQLLTYETVERAIEHRVETKA 499
Query: 429 XTFGQNVTLDDNA 467
FGQ V + A
Sbjct: 498 KIFGQEVNIGAEA 460
Score = 51.9 bits (107), Expect(2) = 1e-021
Identities = 22/46 (47%), Positives = 32/46 (69%)
Frame = +3 / -2
Query: 489 KVDPKVLSKLYDQWVMPLTKDVEVEYLLRRLD*SVITRLAVAANFM 626
K+ P +++ LY +MPLTKDV+V YLLRRLD*++ A +F+
Sbjct: 441 KIRPSLVAGLYSNRIMPLTKDVQVAYLLRRLD*ALTNVQCNACSFV 304
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 374,645,465
Number of Sequences: 312970
Number of extensions: 5869394
Number of successful extensions: 53203
Number of sequences better than 1.0e-020: 10
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 9473341275
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)