TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 3054736.2.1
         (725 letters)

Database: Hordeum_nucl_with_EST.fasta 
           312,970 sequences; 175,134,539 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|BG414688.2|BG414688  HVSMEk0003F21f Hordeum vulgare testa...   130   1e-029
gb|BG416042.1|BG416042  HVSMEk0009I22f Hordeum vulgare testa...    74   1e-027
gb|CK122677.1|CK122677  BES1824105a18 BES1824 Hordeum vulgar...   105   4e-022

>gb|BG414688.2|BG414688 HVSMEk0003F21f Hordeum vulgare testa/pericarp EST library
           HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
           clone HVSMEk0003F21f, mRNA sequence
          Length = 625

 Score =  130 bits (278), Expect = 1e-029
 Identities = 50/95 (52%), Positives = 68/95 (71%)
 Frame = +3 / +3

                                                                       
Query: 69  MHASGSLYALSSEVVEALATAKSDSLRMFDYEDVTIGAWMLAMNVKHEDNRAMCDSVCTP 248
           +HA G +YALS++VV +L   +++S RMF+ EDVTIG+WMLAMNV HE+  A+C+  CT 
Sbjct: 3   LHAYGPIYALSADVVASLVALRNNSFRMFNNEDVTIGSWMLAMNVNHENTHALCEPECTA 182

                                              
Query: 249 TSIAVWDSKKCSGTCNVADKIKQLHNTTVCSKSPT 353
           +SIAVWD  KCSG C+   K+ +LH    C+  PT
Sbjct: 183 SSIAVWDIPKCSGLCHPEVKMLELHRRKECTGGPT 287



>gb|BG416042.1|BG416042 HVSMEk0009I22f Hordeum vulgare testa/pericarp EST library
           HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
           clone HVSMEk0009I22f, mRNA sequence
          Length = 1066

 Score = 73.9 bits (155), Expect(2) = 1e-027
 Identities = 30/56 (53%), Positives = 40/56 (71%)
 Frame = +3 / +2

                                                                   
Query: 3   VVNDPNLKWYESSWELLGNEYFMHASGSLYALSSEVVEALATAKSDSLRMFDYEDV 170
           V  DP LKW E    LLG+EYF+HA G +YALS++VV +L   +++S RMF+ EDV
Sbjct: 50  VFTDPKLKWCEPQSFLLGSEYFLHAYGPIYALSADVVASLVALRNNSFRMFNNEDV 217




 Score = 68.9 bits (144), Expect(2) = 1e-027
 Identities = 26/47 (55%), Positives = 32/47 (68%)
 Frame = +3 / +3

                                                          
Query: 168 VTIGAWMLAMNVKHEDNRAMCDSVCTPTSIAVWDSKKCSGTCNVADK 308
           +TIG+WMLAMNV HE+  A+C+  C   SIA WD  KCSG C+   K
Sbjct: 216 LTIGSWMLAMNVNHENPHALCEPECNGFSIADWDIPKCSGLCHPKGK 356



>gb|CK122677.1|CK122677 BES1824105a18 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
           MPMGp2010A185 5-PRIME, mRNA sequence
          Length = 627

 Score =  105 bits (223), Expect = 4e-022
 Identities = 40/72 (55%), Positives = 51/72 (70%)
 Frame = +3 / +1

                                                                       
Query: 138 DSLRMFDYEDVTIGAWMLAMNVKHEDNRAMCDSVCTPTSIAVWDSKKCSGTCNVADKIKQ 317
           +S RMF+ EDVTIG+WMLAMNV HE+  A+C+  CT +SIAVWD  KCSG C+   K+ +
Sbjct: 1   NSFRMFNNEDVTIGSWMLAMNVNHENTHALCEPECTASSIAVWDIPKCSGLCHPEVKMLE 180

                       
Query: 318 LHNTTVCSKSPT 353
           LH    C+  PT
Sbjct: 181 LHRRKECTGGPT 216



  Database: Hordeum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:16 PM
  Number of letters in database: 175,134,539
  Number of sequences in database:  312,970
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 358,724,864
Number of Sequences: 312970
Number of extensions: 5237476
Number of successful extensions: 41348
Number of sequences better than 1.0e-020: 4
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 8059174710
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)