TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3023930.2.1
(544 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE420972.1|BE420972 HWM004.E01 ITEC HWM Barley Leaf Libr... 191 3e-048
gb|AV836811.1|AV836811 AV836811 K. Sato unpublished cDNA li... 183 5e-046
gb|BE437755.1|BE437755 SFR007.B08F990617 ITEC SFR Barley Le... 160 5e-039
gb|BJ466672.1|BJ466672 BJ466672 K. Sato unpublished cDNA li... 100 7e-035
gb|BJ447915.1|BJ447915 BJ447915 K. Sato unpublished cDNA li... 100 7e-035
gb|BJ455660.1|BJ455660 BJ455660 K. Sato unpublished cDNA li... 100 7e-035
gb|AV917599.1|AV917599 AV917599 K. Sato unpublished cDNA li... 100 7e-035
gb|AV934949.1|AV934949 AV934949 K. Sato unpublished cDNA li... 100 7e-035
gb|AV930332.1|AV930332 AV930332 K. Sato unpublished cDNA li... 100 7e-035
gb|AV927973.1|AV927973 AV927973 K. Sato unpublished cDNA li... 100 7e-035
gb|BM817330.1|BM817330 HC111G07_T3.ab1 HC Hordeum vulgare s... 100 1e-034
gb|BU981962.1|BU981962 HA25B23r HA Hordeum vulgare subsp. v... 98 2e-033
gb|AV930658.1|AV930658 AV930658 K. Sato unpublished cDNA li... 100 1e-029
gb|BI776606.2|BI776606 EBpi07_SQ001_B24_R pistil, 12 DPA, n... 80 2e-028
gb|CA025468.1|CA025468 HZ52E03r HZ Hordeum vulgare subsp. v... 98 1e-023
gb|BF631424.2|BF631424 HVSMEb0015O10f Hordeum vulgare seedl... 68 5e-023
gb|BU971681.1|BU971681 HB19F12r BC Hordeum vulgare subsp. v... 100 7e-022
>gb|BE420972.1|BE420972 HWM004.E01 ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM004.E01, mRNA sequence
Length = 1149
Score = 191 bits (411), Expect = 3e-048
Identities = 75/108 (69%), Positives = 90/108 (83%)
Frame = +1 / +1
Query: 1 YGCPNYWMLHFFKESSGAILHPTAIQVSSYDQLVASAITWQNAKDKSTYLRIKVVNFGNQ 180
YGCPNYWMLHFFK+SSGA HPT +Q+S+YDQ+VASAITWQ+ KDKSTYL+ KVVNF Q
Sbjct: 742 YGCPNYWMLHFFKDSSGATFHPTTMQISNYDQMVASAITWQSPKDKSTYLKNKVVNFRXQ 921
Query: 181 AVDLNISVAELATGVKKSGSKQTVLTSSSPLDENSFQQPEKVAPVSSP 324
AVDLNI+V L +G+K SGS +TVLT +P++ENS P+KVAPV SP
Sbjct: 922 AVDLNITVTGLXSGIKSSGSTKTVLTFXAPVEENSSSHPQKVAPVXSP 1065
Score = 142 bits (304), Expect = 2e-033
Identities = 68/107 (63%), Positives = 77/107 (71%)
Frame = -3 / -2
Query: 323 GLDTGATFSGCWKEFSSSGLLDVRTVCFDPDFLTPVASSATDMFRSTAWLPKFTTFILR* 144
GL TGATF G +EFSS+G +V TV +P+ L P+ S T M RSTA KFTT R*
Sbjct: 1064 GLXTGATFWGWLEEFSSTGAXNVSTVFVEPELLMPLXSPVTVMLRSTACXRKFTTLFFR* 885
Query: 143 VLLSLAFCQVMADATS*S*LET*MAVGWRMAPLDSLKKCSIQ*LGHP 3
VLLSL CQVMADAT *S*LE M VG ++APL+SLKKCSIQ* GHP
Sbjct: 884 VLLSLGLCQVMADATI*S*LEICMVVG*KVAPLESLKKCSIQ*FGHP 744
Score = 117 bits (250), Expect = 5e-026
Identities = 53/114 (46%), Positives = 73/114 (64%)
Frame = +3 / +3
Query: 12 QLLDATLFQGI*RRHPPSYSHSGLQLRSAGCVCHHLAER*RQEHLPQDKGGEFRQPSCGS 191
+LLDATL QG+ RR+ ++H+ LQLRS GC+CHHLAE *RQEHLP+ GGE S GS
Sbjct: 753 KLLDATLLQGLERRNLSPHNHADLQLRSDGCICHHLAEP*RQEHLPEK*GGELPXASRGS 932
Query: 192 EHIRCGTGHRRQEVWIEADGSDVQQSA*RELLPAAREGGASVEPNGERGAADGR 353
+H TG R QE+ ++ + +D++++ RELL +GG +P GR
Sbjct: 933 QHNGHRTGERHQELRLDKNRADIRRARRRELLQPPPKGGPRXKPVAHPQEQKGR 1094
Score = 105 bits (223), Expect(2) = 6e-024
Identities = 48/91 (52%), Positives = 60/91 (65%)
Frame = -2 / -1
Query: 285 GVLVKRTAGRQNRLLRSRLLDAGGQFRNGYVQIHSLVAEIHHLYPEVSALVFSVLPSDGR 106
GVL Q+ R+ LDA Q + YV+IH L+ E+HHL +VSALVF LP DGR
Sbjct: 1026 GVLFDGRXECQHGFCRAGALDAAXQSGDRYVEIHGLLXEVHHLIFQVSALVFRALPGDGR 847
Query: 105 RNQLIVAGDLNGCRMEDGAARFLEKV*HPIV 13
N LIV GDL+GC ++ AAR LE+V*HP+V
Sbjct: 846 CNHLIVVGDLHGCGVKGCAARVLEEV*HPVV 754
Score = 24.9 bits (48), Expect(2) = 6e-024
Identities = 9/24 (37%), Positives = 11/24 (45%)
Frame = -3 / -3
Query: 356 EAPICCPAFAIGLDTGATFSGCWK 285
E P C +A G G F G W+
Sbjct: 1096 ERPFCSWGWATGXKRGPPFGGGWR 1025
>gb|AV836811.1|AV836811 AV836811 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd3o13, mRNA
sequence
Length = 556
Score = 183 bits (395), Expect = 5e-046
Identities = 77/123 (62%), Positives = 98/123 (79%)
Frame = +1 / -1
Query: 37 KESSGAILHPTAIQVSSYDQLVASAITWQNAKDKSTYLRIKVVNFGNQAVDLNISVAELA 216
+ SGA LHP+ IQVS+Y+QLVASAITWQN+KD + YL+IKV+NFGN+AVDL+IS+ L
Sbjct: 553 RTQSGAALHPSTIQVSNYNQLVASAITWQNSKDGNAYLKIKVLNFGNKAVDLSISITGLE 374
Query: 217 TGVKKSGSKQTVLTSSSPLDENSFQQPEKVAPVSSPMANAGQQMGASVGPYSLTSFDLLL 396
G++ GS +TVLTS S DENSFQQP+KV PV SP+ NA ++M + PY+LTSFDLLL
Sbjct: 373 NGIQTFGSIKTVLTSGSLWDENSFQQPDKVVPVESPITNAREEMSLVLDPYALTSFDLLL 194
Query: 397 EPS 405
+ S
Sbjct: 193 DQS 185
Score = 133 bits (285), Expect = 7e-031
Identities = 67/121 (55%), Positives = 75/121 (61%)
Frame = -3 / +3
Query: 407 LLGSSSRSNEVREYGPTEAPICCPAFAIGLDTGATFSGCWKEFSSSGLLDVRTVCFDPDF 228
LL S RSNEVR YG + I A IGL TG T SGCWKEFSS DV TV +P+
Sbjct: 183 LLWSKRRSNEVRAYGSSTRLISSLALVIGLSTGTTLSGCWKEFSSHREPDVSTVFMEPNV 362
Query: 227 LTPVASSATDMFRSTAWLPKFTTFILR*VLLSLAFCQVMADATS*S*LET*MAVGWRMAP 48
P +S ++ RSTA LPKF TFI R* SL FCQV+ADATS * +T*M G AP
Sbjct: 363 *IPFSSPVIEILRSTALLPKFRTFIFR*AFPSLEFCQVIADATSWL*FDT*MVDG*SAAP 542
Query: 47 L 45
L
Sbjct: 543 L 545
>gb|BE437755.1|BE437755 SFR007.B08F990617 ITEC SFR Barley Leaf Epidermis Library Hordeum
vulgare subsp. vulgare cDNA clone SF, mRNA sequence
Length = 522
Score = 160 bits (344), Expect = 5e-039
Identities = 67/107 (62%), Positives = 86/107 (80%)
Frame = +1 / +1
Query: 85 SYDQLVASAITWQNAKDKSTYLRIKVVNFGNQAVDLNISVAELATGVKKSGSKQTVLTSS 264
+Y+QLVASAITWQN+KD + YL+IKV+NFGN+AVDL+IS+ L G++ GS +TVLTS
Sbjct: 4 NYNQLVASAITWQNSKDGNAYLKIKVLNFGNKAVDLSISITGLENGIQTFGSIKTVLTSG 183
Query: 265 SPLDENSFQQPEKVAPVSSPMANAGQQMGASVGPYSLTSFDLLLEPS 405
S DENSFQQP+KV PV SP+ NA ++M + PY+LTSFDLLL+ S
Sbjct: 184 SLWDENSFQQPDKVVPVESPITNAREEMSLVLDPYALTSFDLLLDQS 324
Score = 124 bits (265), Expect = 4e-028
Identities = 59/104 (56%), Positives = 66/104 (63%)
Frame = -3 / -2
Query: 407 LLGSSSRSNEVREYGPTEAPICCPAFAIGLDTGATFSGCWKEFSSSGLLDVRTVCFDPDF 228
LL S RSNEVR YG + I A IGL TG T SGCWKEFSS DV TV +P+
Sbjct: 326 LLWSKRRSNEVRAYGSSTRLISSLALVIGLSTGTTLSGCWKEFSSHREPDVSTVFMEPNV 147
Query: 227 LTPVASSATDMFRSTAWLPKFTTFILR*VLLSLAFCQVMADATS 96
P +S ++ RSTA LPKF TFI R* SL FCQV+ADATS
Sbjct: 146 *IPFSSPVIEILRSTALLPKFRTFIFR*AFPSLEFCQVIADATS 15
>gb|BJ466672.1|BJ466672 BJ466672 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags33f17 3', mRNA sequence
Length = 715
Score = 100 bits (212), Expect(2) = 7e-035
Identities = 43/90 (47%), Positives = 59/90 (65%)
Frame = +1 / -3
Query: 139 STYLRIKVVNFGNQAVDLNISVAELATGVKKSGSKQTVLTSSSPLDENSFQQPEKVAPVS 318
+ +LR+K+VNFG+ V L ISV+ L + GS TVLTSS+ DENSF P KV PV+
Sbjct: 473 NNFLRVKIVNFGSDTVSLTISVSGLQASINALGSNATVLTSSNVKDENSFSNPTKVVPVT 294
Query: 319 SPMANAGQQMGASVGPYSLTSFDLLLEPSK 408
S + NA +QM ++ +S +SFDL L S+
Sbjct: 293 SQLHNAAEQMQVTLAAHSFSSFDLALAQSE 204
Score = 66.6 bits (139), Expect(2) = 7e-035
Identities = 26/52 (50%), Positives = 35/52 (67%)
Frame = +1 / -3
Query: 1 YGCPNYWMLHFFKESSGAILHPTAIQVSSYDQLVASAITWQNAKDKSTYLRI 156
YG P+YWM FF+ESSGA++HP I S L ASAITWQ++ + ++I
Sbjct: 605 YGTPSYWMQKFFRESSGAMIHPITISSSYSGSLAASAITWQDSGNNFLRVKI 450
>gb|BJ447915.1|BJ447915 BJ447915 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak17l17 5', mRNA sequence
Length = 712
Score = 100 bits (212), Expect(2) = 7e-035
Identities = 43/90 (47%), Positives = 59/90 (65%)
Frame = +1 / +1
Query: 139 STYLRIKVVNFGNQAVDLNISVAELATGVKKSGSKQTVLTSSSPLDENSFQQPEKVAPVS 318
+ +LR+K+VNFG+ V L ISV+ L + GS TVLTSS+ DENSF P KV PV+
Sbjct: 349 NNFLRVKIVNFGSDTVSLTISVSGLQASINALGSNATVLTSSNVKDENSFSNPTKVVPVT 528
Query: 319 SPMANAGQQMGASVGPYSLTSFDLLLEPSK 408
S + NA +QM ++ +S +SFDL L S+
Sbjct: 529 SQLHNAAEQMQVTLAAHSFSSFDLALAQSE 618
Score = 66.6 bits (139), Expect(2) = 7e-035
Identities = 26/52 (50%), Positives = 35/52 (67%)
Frame = +1 / +1
Query: 1 YGCPNYWMLHFFKESSGAILHPTAIQVSSYDQLVASAITWQNAKDKSTYLRI 156
YG P+YWM FF+ESSGA++HP I S L ASAITWQ++ + ++I
Sbjct: 217 YGTPSYWMQKFFRESSGAMIHPITISSSYSGSLAASAITWQDSGNNFLRVKI 372
>gb|BJ455660.1|BJ455660 BJ455660 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak17l17 3', mRNA sequence
Length = 704
Score = 100 bits (212), Expect(2) = 7e-035
Identities = 43/90 (47%), Positives = 59/90 (65%)
Frame = +1 / -3
Query: 139 STYLRIKVVNFGNQAVDLNISVAELATGVKKSGSKQTVLTSSSPLDENSFQQPEKVAPVS 318
+ +LR+K+VNFG+ V L ISV+ L + GS TVLTSS+ DENSF P KV PV+
Sbjct: 447 NNFLRVKIVNFGSDTVSLTISVSGLQASINALGSNATVLTSSNVKDENSFSNPTKVVPVT 268
Query: 319 SPMANAGQQMGASVGPYSLTSFDLLLEPSK 408
S + NA +QM ++ +S +SFDL L S+
Sbjct: 267 SQLHNAAEQMQVTLAAHSFSSFDLALAQSE 178
Score = 66.6 bits (139), Expect(2) = 7e-035
Identities = 26/52 (50%), Positives = 35/52 (67%)
Frame = +1 / -3
Query: 1 YGCPNYWMLHFFKESSGAILHPTAIQVSSYDQLVASAITWQNAKDKSTYLRI 156
YG P+YWM FF+ESSGA++HP I S L ASAITWQ++ + ++I
Sbjct: 579 YGTPSYWMQKFFRESSGAMIHPITISSSYSGSLAASAITWQDSGNNFLRVKI 424
>gb|AV917599.1|AV917599 AV917599 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags10j19 3', mRNA sequence
Length = 656
Score = 100 bits (212), Expect(2) = 7e-035
Identities = 43/90 (47%), Positives = 59/90 (65%)
Frame = +1 / -2
Query: 139 STYLRIKVVNFGNQAVDLNISVAELATGVKKSGSKQTVLTSSSPLDENSFQQPEKVAPVS 318
+ +LR+K+VNFG+ V L ISV+ L + GS TVLTSS+ DENSF P KV PV+
Sbjct: 466 NNFLRVKIVNFGSDTVSLTISVSGLQASINALGSNATVLTSSNVKDENSFSNPTKVVPVT 287
Query: 319 SPMANAGQQMGASVGPYSLTSFDLLLEPSK 408
S + NA +QM ++ +S +SFDL L S+
Sbjct: 286 SQLHNAAEQMQVTLAAHSFSSFDLALAQSE 197
Score = 66.6 bits (139), Expect(2) = 7e-035
Identities = 26/52 (50%), Positives = 35/52 (67%)
Frame = +1 / -2
Query: 1 YGCPNYWMLHFFKESSGAILHPTAIQVSSYDQLVASAITWQNAKDKSTYLRI 156
YG P+YWM FF+ESSGA++HP I S L ASAITWQ++ + ++I
Sbjct: 598 YGTPSYWMQKFFRESSGAMIHPITISSSYSGSLAASAITWQDSGNNFLRVKI 443
>gb|AV934949.1|AV934949 AV934949 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal2e14 3', mRNA sequence
Length = 654
Score = 100 bits (212), Expect(2) = 7e-035
Identities = 43/90 (47%), Positives = 59/90 (65%)
Frame = +1 / -1
Query: 139 STYLRIKVVNFGNQAVDLNISVAELATGVKKSGSKQTVLTSSSPLDENSFQQPEKVAPVS 318
+ +LR+K+VNFG+ V L ISV+ L + GS TVLTSS+ DENSF P KV PV+
Sbjct: 465 NNFLRVKIVNFGSDTVSLTISVSGLQASINALGSNATVLTSSNVKDENSFSNPTKVVPVT 286
Query: 319 SPMANAGQQMGASVGPYSLTSFDLLLEPSK 408
S + NA +QM ++ +S +SFDL L S+
Sbjct: 285 SQLHNAAEQMQVTLAAHSFSSFDLALAQSE 196
Score = 66.6 bits (139), Expect(2) = 7e-035
Identities = 26/52 (50%), Positives = 35/52 (67%)
Frame = +1 / -1
Query: 1 YGCPNYWMLHFFKESSGAILHPTAIQVSSYDQLVASAITWQNAKDKSTYLRI 156
YG P+YWM FF+ESSGA++HP I S L ASAITWQ++ + ++I
Sbjct: 597 YGTPSYWMQKFFRESSGAMIHPITISSSYSGSLAASAITWQDSGNNFLRVKI 442
>gb|AV930332.1|AV930332 AV930332 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd22b16 3', mRNA sequence
Length = 646
Score = 100 bits (212), Expect(2) = 7e-035
Identities = 43/90 (47%), Positives = 59/90 (65%)
Frame = +1 / -2
Query: 139 STYLRIKVVNFGNQAVDLNISVAELATGVKKSGSKQTVLTSSSPLDENSFQQPEKVAPVS 318
+ +LR+K+VNFG+ V L ISV+ L + GS TVLTSS+ DENSF P KV PV+
Sbjct: 456 NNFLRVKIVNFGSDTVSLTISVSGLQASINALGSNATVLTSSNVKDENSFSNPTKVVPVT 277
Query: 319 SPMANAGQQMGASVGPYSLTSFDLLLEPSK 408
S + NA +QM ++ +S +SFDL L S+
Sbjct: 276 SQLHNAAEQMQVTLAAHSFSSFDLALAQSE 187
Score = 66.6 bits (139), Expect(2) = 7e-035
Identities = 26/52 (50%), Positives = 35/52 (67%)
Frame = +1 / -2
Query: 1 YGCPNYWMLHFFKESSGAILHPTAIQVSSYDQLVASAITWQNAKDKSTYLRI 156
YG P+YWM FF+ESSGA++HP I S L ASAITWQ++ + ++I
Sbjct: 588 YGTPSYWMQKFFRESSGAMIHPITISSSYSGSLAASAITWQDSGNNFLRVKI 433
>gb|AV927973.1|AV927973 AV927973 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd13h16 3', mRNA sequence
Length = 642
Score = 100 bits (212), Expect(2) = 7e-035
Identities = 43/90 (47%), Positives = 59/90 (65%)
Frame = +1 / -2
Query: 139 STYLRIKVVNFGNQAVDLNISVAELATGVKKSGSKQTVLTSSSPLDENSFQQPEKVAPVS 318
+ +LR+K+VNFG+ V L ISV+ L + GS TVLTSS+ DENSF P KV PV+
Sbjct: 452 NNFLRVKIVNFGSDTVSLTISVSGLQASINALGSNATVLTSSNVKDENSFSNPTKVVPVT 273
Query: 319 SPMANAGQQMGASVGPYSLTSFDLLLEPSK 408
S + NA +QM ++ +S +SFDL L S+
Sbjct: 272 SQLHNAAEQMQVTLAAHSFSSFDLALAQSE 183
Score = 66.6 bits (139), Expect(2) = 7e-035
Identities = 26/52 (50%), Positives = 35/52 (67%)
Frame = +1 / -2
Query: 1 YGCPNYWMLHFFKESSGAILHPTAIQVSSYDQLVASAITWQNAKDKSTYLRI 156
YG P+YWM FF+ESSGA++HP I S L ASAITWQ++ + ++I
Sbjct: 584 YGTPSYWMQKFFRESSGAMIHPITISSSYSGSLAASAITWQDSGNNFLRVKI 429
>gb|BM817330.1|BM817330 HC111G07_T3.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC111G07_T3.ab1 similar to (AC011708) putative
alpha-L-arabinofuranosidase [Arabidopsis thaliana], mRNA
sequence
Length = 924
Score = 99.6 bits (211), Expect(2) = 1e-034
Identities = 43/90 (47%), Positives = 58/90 (64%)
Frame = +1 / +3
Query: 139 STYLRIKVVNFGNQAVDLNISVAELATGVKKSGSKQTVLTSSSPLDENSFQQPEKVAPVS 318
+ +LR+K+VNFG+ V L ISV+ L + GS TVLTSS+ DENSF P KV PV+
Sbjct: 495 NNFLRVKIVNFGSDTVSLTISVSGLQASINALGSNATVLTSSNVKDENSFSNPTKVVPVT 674
Query: 319 SPMANAGQQMGASVGPYSLTSFDLLLEPSK 408
S + NA +QM ++ +S SFDL L S+
Sbjct: 675 SQLHNAAEQMQVTLAAHSFXSFDLALAQSE 764
Score = 66.6 bits (139), Expect(2) = 1e-034
Identities = 26/52 (50%), Positives = 35/52 (67%)
Frame = +1 / +3
Query: 1 YGCPNYWMLHFFKESSGAILHPTAIQVSSYDQLVASAITWQNAKDKSTYLRI 156
YG P+YWM FF+ESSGA++HP I S L ASAITWQ++ + ++I
Sbjct: 363 YGTPSYWMQKFFRESSGAMIHPITISSSYSGSLAASAITWQDSGNNFLRVKI 518
>gb|BU981962.1|BU981962 HA25B23r HA Hordeum vulgare subsp. vulgare cDNA clone HA25B23
5-PRIME, mRNA sequence
Length = 558
Score = 98.2 bits (208), Expect(2) = 2e-033
Identities = 43/89 (48%), Positives = 58/89 (65%)
Frame = +1 / +1
Query: 133 DKSTYLRIKVVNFGNQAVDLNISVAELATGVKKSGSKQTVLTSSSPLDENSFQQPEKVAP 312
++ +LRIK+VNFG+ AV+L +S L GV S S TVLTS + LDEN+F P V P
Sbjct: 169 NEDIFLRIKIVNFGSNAVNLTLSAIGLQAGVNTSRSAVTVLTSDNVLDENTFDDPRNVVP 348
Query: 313 VSSPMANAGQQMGASVGPYSLTSFDLLLE 399
S + NA ++M A + P+S TSFDL L+
Sbjct: 349 ERSDLPNAAEEMQAMLVPHSFTSFDLALD 435
Score = 63.8 bits (133), Expect(2) = 2e-033
Identities = 24/45 (53%), Positives = 31/45 (68%)
Frame = +1 / +1
Query: 1 YGCPNYWMLHFFKESSGAILHPTAIQVSSYDQLVASAITWQNAKD 135
YG P+YWM FF+ESSGA++HP + S L ASAITW + +D
Sbjct: 43 YGTPSYWMQTFFRESSGAVIHPMRLNSSYSGSLAASAITWGDNED 177
>gb|AV930658.1|AV930658 AV930658 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd24j13 3', mRNA sequence
Length = 561
Score = 100 bits (212), Expect(2) = 1e-029
Identities = 43/90 (47%), Positives = 59/90 (65%)
Frame = +1 / -1
Query: 139 STYLRIKVVNFGNQAVDLNISVAELATGVKKSGSKQTVLTSSSPLDENSFQQPEKVAPVS 318
+ +LR+K+VNFG+ V L ISV+ L + GS TVLTSS+ DENSF P KV PV+
Sbjct: 447 NNFLRVKIVNFGSDTVSLTISVSGLQASINALGSNATVLTSSNVKDENSFSNPTKVVPVT 268
Query: 319 SPMANAGQQMGASVGPYSLTSFDLLLEPSK 408
S + NA +QM ++ +S +SFDL L S+
Sbjct: 267 SQLHNAAEQMQVTLAAHSFSSFDLALAQSE 178
Score = 49.2 bits (101), Expect(2) = 1e-029
Identities = 21/45 (46%), Positives = 29/45 (64%)
Frame = +1 / -1
Query: 22 MLHFFKESSGAILHPTAIQVSSYDQLVASAITWQNAKDKSTYLRI 156
M FF+ESSGA++HP I S L ASAITWQ++ + ++I
Sbjct: 558 MQKFFRESSGAMIHPITISSSYSGSLAASAITWQDSGNNFLRVKI 424
>gb|BI776606.2|BI776606 EBpi07_SQ001_B24_R pistil, 12 DPA, no treatment, cv Optic, EBpi07
Hordeum vulgare subsp. vulgare cDNA clone
EBpi07_SQ001_B24 5', mRNA sequence
Length = 453
Score = 80.3 bits (169), Expect(2) = 2e-028
Identities = 36/76 (47%), Positives = 48/76 (63%)
Frame = +1 / +2
Query: 145 YLRIKVVNFGNQAVDLNISVAELATGVKKSGSKQTVLTSSSPLDENSFQQPEKVAPVSSP 324
+LRIK+VNFG+ AV+L +S L GV S TVLTS + LDEN+F P V P S
Sbjct: 221 FLRIKIVNFGSNAVNLTLSAIGLQAGVNT*RSAVTVLTSDNVLDENTFDDPRNVVPERSD 400
Query: 325 MANAGQQMGASVGPYS 372
+ NA ++M A + P+S
Sbjct: 401 LPNAAEEMQAMLVPHS 448
Score = 64.8 bits (135), Expect(2) = 2e-028
Identities = 24/45 (53%), Positives = 32/45 (71%)
Frame = +1 / +2
Query: 1 YGCPNYWMLHFFKESSGAILHPTAIQVSSYDQLVASAITWQNAKD 135
YG P+YWM FF+ESSGA++HP + S L ASAITW +++D
Sbjct: 83 YGTPSYWMHTFFRESSGAVIHPMRLNSSYSGSLAASAITWGDSED 217
>gb|CA025468.1|CA025468 HZ52E03r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ52E03
5-PRIME, mRNA sequence
Length = 462
Score = 98.2 bits (208), Expect(2) = 1e-023
Identities = 43/89 (48%), Positives = 58/89 (65%)
Frame = +1 / +1
Query: 133 DKSTYLRIKVVNFGNQAVDLNISVAELATGVKKSGSKQTVLTSSSPLDENSFQQPEKVAP 312
++ +LRIK+VNFG+ AV+L +S L GV S S TVLTS + LDEN+F P V P
Sbjct: 85 NEDIFLRIKIVNFGSNAVNLTLSAIGLQAGVNTSRSAVTVLTSDNVLDENTFDDPRNVVP 264
Query: 313 VSSPMANAGQQMGASVGPYSLTSFDLLLE 399
S + NA ++M A + P+S TSFDL L+
Sbjct: 265 ERSDLPNAAEEMQAMLVPHSFTSFDLALD 351
Score = 30.9 bits (61), Expect(2) = 1e-023
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = +1 / +1
Query: 52 AILHPTAIQVSSYDQLVASAITWQNAKD 135
A++HP + S L ASAITW + +D
Sbjct: 10 AVIHPMRLNSSYSGSLAASAITWGDNED 93
>gb|BF631424.2|BF631424 HVSMEb0015O10f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0015O10f, mRNA sequence
Length = 762
Score = 67.5 bits (141), Expect(2) = 5e-023
Identities = 25/43 (58%), Positives = 32/43 (74%)
Frame = +1 / +2
Query: 1 YGCPNYWMLHFFKESSGAILHPTAIQVSSYDQLVASAITWQNA 129
YG P+YWM FF+ESSGA++HP AI S L ASA+TWQ++
Sbjct: 149 YGTPSYWMQKFFRESSGAMIHPIAISSSYSGSLTASAVTWQDS 277
Score = 59.3 bits (123), Expect(2) = 5e-023
Identities = 29/75 (38%), Positives = 42/75 (56%)
Frame = +1 / +2
Query: 148 LRIKVVNFGNQAVDLNISVAELATGVKKSGSKQTVLTSSSPLDENSFQQPEKVAPVSSPM 327
LR+K+ N G+ L I V L T + GS TVLT +S DENS + P K P +S +
Sbjct: 290 LRVKIEN*GSDTESLTICVTGLQTDINALGSNATVLTCNSEEDENSLKDPTKDMPGTSQV 469
Query: 328 ANAGQQMGASVGPYS 372
+A +QM ++ +S
Sbjct: 470 HSARKQMHVTLATHS 514
>gb|BU971681.1|BU971681 HB19F12r BC Hordeum vulgare subsp. vulgare cDNA clone HB19F12
5-PRIME, mRNA sequence
Length = 556
Score = 100 bits (212), Expect(2) = 7e-022
Identities = 43/90 (47%), Positives = 59/90 (65%)
Frame = +1 / +2
Query: 139 STYLRIKVVNFGNQAVDLNISVAELATGVKKSGSKQTVLTSSSPLDENSFQQPEKVAPVS 318
+ +LR+K+VNFG+ V L ISV+ L + GS TVLTSS+ DENSF P KV PV+
Sbjct: 83 NNFLRVKIVNFGSDTVSLTISVSGLQASINALGSNATVLTSSNVKDENSFSNPTKVVPVT 262
Query: 319 SPMANAGQQMGASVGPYSLTSFDLLLEPSK 408
S + NA +QM ++ +S +SFDL L S+
Sbjct: 263 SQLHNAAEQMQVTLAAHSFSSFDLALAQSE 352
Score = 23.1 bits (44), Expect(2) = 7e-022
Identities = 9/20 (45%), Positives = 14/20 (70%)
Frame = +1 / +2
Query: 97 LVASAITWQNAKDKSTYLRI 156
L ASAITWQ++ + ++I
Sbjct: 47 LAASAITWQDSGNNFLRVKI 106
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 270,193,028
Number of Sequences: 312970
Number of extensions: 4383639
Number of successful extensions: 80966
Number of sequences better than 1.0e-020: 22
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 5554858270
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)