TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3023309.2.1
(1096 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE420728.1|BE420728 HWM001.H09 ITEC HWM Barley Leaf Libr... 224 8e-088
gb|BF621115.2|BF621115 HVSMEa0004B20f Hordeum vulgare seedl... 224 3e-083
gb|BU969261.1|BU969261 HB11A08r BC Hordeum vulgare subsp. v... 210 9e-078
gb|BJ547861.1|BJ547861 BJ547861 K. Sato unpublished cDNA li... 224 3e-073
gb|BJ547531.1|BJ547531 BJ547531 K. Sato unpublished cDNA li... 224 7e-072
gb|BJ548991.1|BJ548991 BJ548991 K. Sato unpublished cDNA li... 200 7e-067
gb|BU979069.1|BU979069 HA15B05r HA Hordeum vulgare subsp. v... 224 2e-066
gb|AL503599.1|AL503599 AL503599 Hordeum vulgare Barke roots... 221 1e-064
gb|BU967835.1|BU967835 HB05I11r BC Hordeum vulgare subsp. v... 171 5e-064
gb|BF619204.2|BF619204 HVSMEc0015B21f Hordeum vulgare seedl... 194 2e-062
gb|BM816161.1|BM816161 HC108D04_SK.ab1 HC Hordeum vulgare s... 139 4e-059
gb|BU979480.1|BU979480 HA16E20r HA Hordeum vulgare subsp. v... 224 8e-058
gb|BM816160.1|BM816160 HC108D03_SK.ab1 HC Hordeum vulgare s... 139 3e-057
gb|BE601776.2|BE601776 HVSMEh0099I05f Hordeum vulgare 5-45 ... 221 4e-057
gb|CA001791.1|CA001791 HS05J13r HS Hordeum vulgare subsp. v... 218 4e-056
gb|BF623183.2|BF623183 HVSMEa0015A20f Hordeum vulgare seedl... 138 7e-052
gb|AL501018.1|AL501018 AL501018 Hordeum vulgare Barke roots... 193 7e-052
gb|CA001055.1|CA001055 HS05J13u HS Hordeum vulgare subsp. v... 193 1e-051
gb|BM368467.2|BM368467 EBem08_SQ002_G06_R embryo, 40 DPA, n... 132 2e-046
gb|CA025379.1|CA025379 HZ51P24r HZ Hordeum vulgare subsp. v... 139 3e-046
gb|AV920834.1|AV920834 AV920834 K. Sato unpublished cDNA li... 136 5e-044
gb|BU974881.1|BU974881 HB29G07r BC Hordeum vulgare subsp. v... 173 2e-042
gb|BM097565.2|BM097565 EBem04_SQ002_B01_R embryo, 12 DPA, n... 139 1e-037
gb|BG299708.1|BG299708 HVSMEa0021H20f Hordeum vulgare seedl... 132 4e-037
gb|AL505772.1|AL505772 AL505772 Hordeum vulgare Barke roots... 139 3e-032
gb|AV915725.1|AV915725 AV915725 K. Sato unpublished cDNA li... 139 3e-032
gb|CA000585.1|CA000585 HS08C04u HS Hordeum vulgare subsp. v... 136 3e-031
gb|BQ761571.1|BQ761571 EBem10_SQ001_K04_R embryo, 2 Day ger... 115 6e-025
>gb|BE420728.1|BE420728 HWM001.H09 ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM001.H09, mRNA sequence
Length = 1193
Score = 224 bits (483), Expect(4) = 8e-088
Identities = 93/128 (72%), Positives = 102/128 (79%)
Frame = +2 / +3
Query: 116 IPPNVTIYXXXXXXXXXXXXXXXSLTAVFSQFGKILDVLAFKTLKHKGQAWVVFEDVASA 295
+ PNVTIY SL AVFSQFGKI++VLAFKTLKHKGQAWVVFEDVASA
Sbjct: 156 VQPNVTIYINNLNEKIKLDALKKSLNAVFSQFGKIIEVLAFKTLKHKGQAWVVFEDVASA 335
Query: 296 TEALKRMQGFPFYDKTMRIQYAKTKSDIIAKADGTFVPRERRKRTDEKSEKKQKREHHHD 475
TEALKRMQ FPFYDK M+IQYAKTKSD++AKADGTFVPRE+RKR DEK EKKQKRE HHD
Sbjct: 336 TEALKRMQDFPFYDKPMKIQYAKTKSDVVAKADGTFVPREKRKRNDEKPEKKQKREQHHD 515
Query: 476 AGASQIGM 499
+ +GM
Sbjct: 516 VNQAGVGM 539
Score = 182 bits (392), Expect(4) = 8e-066
Identities = 81/132 (61%), Positives = 96/132 (72%)
Frame = -1 / -1
Query: 499 HANLTSTSIMVMLALLLLFRFFISPLSSFSRYKGAISLCYNI*FGLGILNSHSFVIEGKA 320
HA+ + +I+V+L LLLLFRF I PL FSRY+GAIS CYNI* GLGILN H FVIEG+
Sbjct: 539 HAHASLVNIVVLLTLLLLFRFLIIPLPFFSRYEGAISFCYNI*LGLGILNFHGFVIEGEI 360
Query: 319 LHPLQSLSS*CDIFEDNPSLTFMLQSLESKHIKNFPXXXXXXXXXXXKFFELDLLVEVVD 140
LHPLQSL *C+IFE +P L FMLQSL+SKH+ +FP K E DLLVEV+D
Sbjct: 359 LHPLQSLGC*CNIFEHDPCLPFMLQSLKSKHLNDFPKLGENRIEGLLKCVEFDLLVEVID 180
Query: 139 IDGDVRRDAIRL 104
+DG V DA+RL
Sbjct: 179 VDGHVWLDAVRL 144
Score = 157 bits (338), Expect(2) = 2e-044
Identities = 76/120 (63%), Positives = 84/120 (70%)
Frame = +1 / +2
Query: 118 PAERHHLYQQPQREDQARRT*EVPNSRFFPVWENS*CACFQDFEAXXXXXXXXXXXXISY 297
PA+R HL+Q PQREDQ RRT*EVP F PVWEN * ACF DFEA IS
Sbjct: 158 PAKRDHLHQ*PQREDQTRRT*EVPQCGFLPVWENH*GACF*DFEA*RASMGRVRRCCISN 337
Query: 298 *GSEEDAGLSLL*QNYENSICQDQIRYYSKG*WHLCTSRKKKED**KI*KEAEARASP*C 477
G EEDA LL*Q +ENSICQDQ+R SK *WHL TSRKK+E+**+ *KEAEA A+P C
Sbjct: 338 RGFEEDARFPLL*QTHENSICQDQVRCCSKS*WHLRTSRKKEEE**ET*KEAEA*AAPRC 517
Score = 150 bits (323), Expect(2) = 8e-037
Identities = 76/135 (56%), Positives = 86/135 (63%)
Frame = -3 / -3
Query: 521 HQGKQLHPCQFD*HQHHGDARXXXXXXXXXXXXXXFLEVQRCHQPLL*YLIWSWHIEFS* 342
H+ + H C H G A FLEV+RCHQ LL +L WSWHIEFS
Sbjct: 561 HKHQDRHACPRQPG*HRGAAHASASFQVSHHSSSFFLEVRRCHQLLLQHLTWSWHIEFSW 382
Query: 341 FCHRRESPASSSEPQ*LMRHLRRQPKLDLYASKS*KQAHQEFSQTGKKRLLGTS*VLRA* 162
CHRR + ASSS+P+ LM+HLR +P L LYASKS*KQA Q FSQTG+K GTS*V R *
Sbjct: 381 VCHRRGNLASSSKPRLLMQHLRTRPMLALYASKS*KQAPQ*FSQTGRKPH*GTS*VRRV* 202
Query: 161 SSR*GC*YRW*RSAG 117
SSR*G * RW R AG
Sbjct: 201 SSR*GY*CRWSRLAG 157
Score = 149 bits (319), Expect(3) = 5e-055
Identities = 70/97 (72%), Positives = 73/97 (75%)
Frame = -2 / -2
Query: 411 RGTKVPSAFAIISDLVLAY*ILIVLS*KGKPCILFRASVADATSSKTTQA*PLCFKVLKA 232
RGTKVPSAFA SDLVLAY*I + LS*KGK CILF+ASVADATSS TT A PLCFKVLKA
Sbjct: 451 RGTKVPSAFATTSDLVLAY*IFMGLS*KGKSCILFKASVADATSSNTTHACPLCFKVLKA 272
Query: 231 STSRIFPNWEKTAVRDXXXXXXXXXXXXXXX*MVTFG 121
STS IFPNWEKTA+RD *MVTFG
Sbjct: 271 STSMIFPNWEKTALRDFLSASSLIFSLRLLM*MVTFG 161
Score = 146 bits (313), Expect = 2e-034
Identities = 72/124 (58%), Positives = 81/124 (65%)
Frame = +3 / +1
Query: 105 RRMAXXXXXXXXXXXXXXXXXXKNLRSP*QPFFPSLGKFLMCLLSRL*SIKVKLGLSSKM 284
RR A +LRSP F PSLGK L CLL RL*SIK K G SKM
Sbjct: 145 RRTASSQT*PSTSITSTRRSNSTHLRSPSMRFSPSLGKSLRCLLLRL*SIKGKHGSCSKM 324
Query: 285 SHQLLRL*RGCRAFPSMTKL*EFNMPRPNQIL*QRLMAPLYLEKEERGLMKNLKRSRSAS 464
HQ RL*RGC+ PSMT *+FNMPRP+Q+L*Q+LMAP YLEK+ RG+M+NLKRSRS S
Sbjct: 325 LHQQPRL*RGCKISPSMTNP*KFNMPRPSQML*QKLMAPSYLEKKGRGMMRNLKRSRSVS 504
Query: 465 ITMM 476
T M
Sbjct: 505 STTM 516
Score = 91.8 bits (194), Expect(4) = 8e-088
Identities = 36/48 (75%), Positives = 40/48 (83%)
Frame = +2 / +1
Query: 563 GQRVMMPEIIVPNNILFVQNLPHETTPMMLQMLFCQYPGFKEVRMIEA 706
G MMP+IIVPNNILFVQ LPH+ T +MLQM FCQ+PGFKEV MIEA
Sbjct: 595 GTEAMMPDIIVPNNILFVQELPHDATALMLQMSFCQFPGFKEVPMIEA 738
Score = 77.1 bits (162), Expect(4) = 8e-066
Identities = 32/49 (65%), Positives = 35/49 (71%)
Frame = -2 / -1
Query: 708 FASIIRTSLKPGYWQKSIWSIMGVVSCGKFWTNKMLFGTIISGIMTLCP 562
FASII TSL PG WQK I SI V SCG WT ++LFGTIISGI+ P
Sbjct: 740 FASIIGTSLNPGNWQKDICSIKAVASCGSSWTKRILFGTIISGIIASVP 594
Score = 71.2 bits (149), Expect(3) = 5e-055
Identities = 23/42 (54%), Positives = 32/42 (76%)
Frame = -1 / -3
Query: 700 DHPNLLEARVLAEEHLEHHGRRLVWQVLDKQDVVWYDNLWHH 575
DH N LE LAE HL+H R+VW+ LD++++VWY+N+WHH
Sbjct: 732 DHRNFLEPWELAEGHLQHQSGRVVWEFLDEENIVWYNNIWHH 607
Score = 41.8 bits (85), Expect(2) = 2e-044
Identities = 19/49 (38%), Positives = 26/49 (53%)
Frame = +1 / +3
Query: 562 RTKGHDARDYRTKQHLVCPKLATRXXXXXXXXXXXXVPWLQGGSDDRGE 708
R + +DAR Y TKQ+ + P+ TR VP +QG SDDR +
Sbjct: 594 RDRSYDARYYCTKQYSLRPRTPTRRDRFDAADVLLPVPRVQGSSDDRSK 740
Score = 38.6 bits (78), Expect(4) = 8e-088
Identities = 12/18 (66%), Positives = 12/18 (66%)
Frame = +1 / +2
Query: 481 C*SNWHGCNCLPWCLWGS 534
C* W G CL WCLWGS
Sbjct: 515 C*PGWRGHACLSWCLWGS 568
Score = 33.6 bits (67), Expect(3) = 5e-055
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = -1 / -1
Query: 532 SPINTRVSSYTHANLTSTSIMVMLALLLLFRFFISPLSS 416
SPI+TR+ + HA+L + +++ L LL F P S
Sbjct: 566 SPISTRIGMHAHASLVNIVVLLTLLLLFRFLIIPLPFFS 450
Score = 28.1 bits (55), Expect(4) = 8e-088
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = +2 / +2
Query: 719 AFVEYGDETQAT 754
AFVEYGDE QAT
Sbjct: 749 AFVEYGDEGQAT 784
Score = 25.8 bits (50), Expect(4) = 8e-066
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = -3 / -3
Query: 539 AVEPHKHQGKQLHPCQFD*HQ 477
+ EPHKHQ + P Q *H+
Sbjct: 573 SAEPHKHQDRHACPRQPG*HR 511
Score = 23.5 bits (45), Expect(2) = 8e-037
Identities = 15/43 (34%), Positives = 18/43 (41%)
Frame = -3 / -2
Query: 701 RSSEPP*SQXXXXXXXXXXXXXXXXXSFGQTRCCLVR*SLAS* 573
RSSE P*+ G+ CLV+* LAS*
Sbjct: 733 RSSELP*TLGTGRRTSAASKRSRRVGVLGRREYCLVQ*YLAS* 605
Score = 23.1 bits (44), Expect(4) = 8e-066
Identities = 10/12 (83%), Positives = 10/12 (83%)
Frame = -2 / -3
Query: 753 VAWVSSPYSTKA 718
VA SSPYSTKA
Sbjct: 783 VACPSSPYSTKA 748
>gb|BF621115.2|BF621115 HVSMEa0004B20f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0004B20f, mRNA sequence
Length = 792
Score = 224 bits (483), Expect(2) = 3e-083
Identities = 93/128 (72%), Positives = 102/128 (79%)
Frame = +2 / +1
Query: 116 IPPNVTIYXXXXXXXXXXXXXXXSLTAVFSQFGKILDVLAFKTLKHKGQAWVVFEDVASA 295
+ PNVTIY SL AVFSQFGKI++VLAFKTLKHKGQAWVVFEDVASA
Sbjct: 103 VQPNVTIYINNLNEKIKLDALKKSLNAVFSQFGKIIEVLAFKTLKHKGQAWVVFEDVASA 282
Query: 296 TEALKRMQGFPFYDKTMRIQYAKTKSDIIAKADGTFVPRERRKRTDEKSEKKQKREHHHD 475
TEALKRMQ FPFYDK M+IQYAKTKSD++AKADGTFVPRE+RKR DEK EKKQKRE HHD
Sbjct: 283 TEALKRMQDFPFYDKPMKIQYAKTKSDVVAKADGTFVPREKRKRNDEKPEKKQKREQHHD 462
Query: 476 AGASQIGM 499
+ +GM
Sbjct: 463 VNQAGVGM 486
Score = 183 bits (394), Expect(2) = 2e-079
Identities = 82/136 (60%), Positives = 98/136 (72%)
Frame = -1 / -1
Query: 499 HANLTSTSIMVMLALLLLFRFFISPLSSFSRYKGAISLCYNI*FGLGILNSHSFVIEGKA 320
HA+ + +I+V+L LLLLFRF I PL FSRY+GAIS CYNI* GLGILN H FVIEG+
Sbjct: 486 HAHASLVNIVVLLTLLLLFRFLIIPLPFFSRYEGAISFCYNI*LGLGILNFHGFVIEGEI 307
Query: 319 LHPLQSLSS*CDIFEDNPSLTFMLQSLESKHIKNFPXXXXXXXXXXXKFFELDLLVEVVD 140
LHPLQSL *C+IFE +P L FMLQSL+SKH+ +FP K E DLLVEV+D
Sbjct: 306 LHPLQSLGC*CNIFEHDPCLPFMLQSLKSKHLNDFPKLGENRIEGLLKCVEFDLLVEVID 127
Query: 139 IDGDVRRDAIRLSFAS 92
+DG V DA+RL A+
Sbjct: 126 VDGHVWLDAVRLPAAA 79
Score = 179 bits (385), Expect = 3e-044
Identities = 74/124 (59%), Positives = 89/124 (71%)
Frame = +2 / +1
Query: 416 RRKRTDEKSEKKQKREHHHDAGASQIGMGVTAYPGVYGAPQLTQIPIAGGQRVMMPEIIV 595
R KR + ++K++ +Q G+G+ AYPG YGAP L+Q+P G +VMMPEIIV
Sbjct: 397 REKRKRNDEKPEKKQKREQHHDVNQAGVGMHAYPGAYGAPPLSQLPFGAGPKVMMPEIIV 576
Query: 596 PNNILFVQNLPHETTPMMLQMLFCQYPGFKEVRMIEAKPGIAFVEYGDETQATAAMNNLQ 775
PNNILFVQ LPH+ T +MLQM FCQYPGFK VRMIEA PGIAFVEYGD+ ATAA+
Sbjct: 577 PNNILFVQELPHDATALMLQMFFCQYPGFKGVRMIEAXPGIAFVEYGDKGTATAAIEPS* 756
Query: 776 SFKI 787
FKI
Sbjct: 757 GFKI 768
Score = 157 bits (338), Expect(3) = 6e-044
Identities = 76/120 (63%), Positives = 84/120 (70%)
Frame = +1 / +3
Query: 118 PAERHHLYQQPQREDQARRT*EVPNSRFFPVWENS*CACFQDFEAXXXXXXXXXXXXISY 297
PA+R HL+Q PQREDQ RRT*EVP F PVWEN * ACF DFEA IS
Sbjct: 105 PAKRDHLHQ*PQREDQTRRT*EVPQCGFLPVWENH*GACF*DFEA*RASMGRVRRCCISN 284
Query: 298 *GSEEDAGLSLL*QNYENSICQDQIRYYSKG*WHLCTSRKKKED**KI*KEAEARASP*C 477
G EEDA LL*Q +ENSICQDQ+R SK *WHL TSRKK+E+**+ *KEAEA A+P C
Sbjct: 285 RGFEEDARFPLL*QTHENSICQDQVRCCSKS*WHLRTSRKKEEE**ET*KEAEA*AAPRC 464
Score = 150 bits (323), Expect(2) = 5e-038
Identities = 76/135 (56%), Positives = 86/135 (63%)
Frame = -3 / -3
Query: 521 HQGKQLHPCQFD*HQHHGDARXXXXXXXXXXXXXXFLEVQRCHQPLL*YLIWSWHIEFS* 342
H+ + H C H G A FLEV+RCHQ LL +L WSWHIEFS
Sbjct: 508 HKHQDRHACPRQPG*HRGAAHASASFQVSHHSSSFFLEVRRCHQLLLQHLTWSWHIEFSW 329
Query: 341 FCHRRESPASSSEPQ*LMRHLRRQPKLDLYASKS*KQAHQEFSQTGKKRLLGTS*VLRA* 162
CHRR + ASSS+P+ LM+HLR +P L LYASKS*KQA Q FSQTG+K GTS*V R *
Sbjct: 328 VCHRRGNLASSSKPRLLMQHLRTRPMLALYASKS*KQAPQ*FSQTGRKPH*GTS*VRRV* 149
Query: 161 SSR*GC*YRW*RSAG 117
SSR*G * RW R AG
Sbjct: 148 SSR*GY*CRWSRLAG 104
Score = 149 bits (319), Expect(2) = 3e-070
Identities = 70/97 (72%), Positives = 73/97 (75%)
Frame = -2 / -2
Query: 411 RGTKVPSAFAIISDLVLAY*ILIVLS*KGKPCILFRASVADATSSKTTQA*PLCFKVLKA 232
RGTKVPSAFA SDLVLAY*I + LS*KGK CILF+ASVADATSS TT A PLCFKVLKA
Sbjct: 398 RGTKVPSAFATTSDLVLAY*IFMGLS*KGKSCILFKASVADATSSNTTHACPLCFKVLKA 219
Query: 231 STSRIFPNWEKTAVRDXXXXXXXXXXXXXXX*MVTFG 121
STS IFPNWEKTA+RD *MVTFG
Sbjct: 218 STSMIFPNWEKTALRDFLSASSLIFSLRLLM*MVTFG 108
Score = 146 bits (313), Expect = 2e-034
Identities = 72/124 (58%), Positives = 81/124 (65%)
Frame = +3 / +2
Query: 105 RRMAXXXXXXXXXXXXXXXXXXKNLRSP*QPFFPSLGKFLMCLLSRL*SIKVKLGLSSKM 284
RR A +LRSP F PSLGK L CLL RL*SIK K G SKM
Sbjct: 92 RRTASSQT*PSTSITSTRRSNSTHLRSPSMRFSPSLGKSLRCLLLRL*SIKGKHGSCSKM 271
Query: 285 SHQLLRL*RGCRAFPSMTKL*EFNMPRPNQIL*QRLMAPLYLEKEERGLMKNLKRSRSAS 464
HQ RL*RGC+ PSMT *+FNMPRP+Q+L*Q+LMAP YLEK+ RG+M+NLKRSRS S
Sbjct: 272 LHQQPRL*RGCKISPSMTNP*KFNMPRPSQML*QKLMAPSYLEKKGRGMMRNLKRSRSVS 451
Query: 465 ITMM 476
T M
Sbjct: 452 STTM 463
Score = 137 bits (293), Expect(2) = 3e-070
Identities = 58/124 (46%), Positives = 79/124 (63%)
Frame = -1 / -1
Query: 787 NFEALKVVHGSSGLGLVSILHESNPRLRLDHPNLLEARVLAEEHLEHHGRRLVWQVLDKQ 608
+FE L+ +G LVSILH+SN RL DHP+ E VLAEEHL+H R+VW+ LD++
Sbjct: 768 DFEPLRRFNGCGRCSLVSILHKSNTRLCFDHPHSFEPWVLAEEHLQHQSGRVVWEFLDEE 589
Query: 607 DVVWYDNLWHHDPLSSCYRYLGELWSPINTRVSSYTHANLTSTSIMVMLALLLLFRFFIS 428
++VWY+NLWHHD S R L E SPI+TR+ + HA+L + +++ L LL F
Sbjct: 588 NIVWYNNLWHHDLRSRTKR*LRERRSPISTRIGMHAHASLVNIVVLLTLLLLFRFLIIPL 409
Query: 427 PLSS 416
P S
Sbjct: 408 PFFS 397
Score = 133 bits (285), Expect(2) = 2e-079
Identities = 64/99 (64%), Positives = 68/99 (68%)
Frame = -2 / -2
Query: 789 VILKL*RLFMAAVAWVSSPYSTKAIPGFASIIRTSLKPGYWQKSIWSIMGVVSCGKFWTN 610
+IL * MAAVA SPYSTKAIPG ASIIRT L PGYWQK+I SI V SCG WT
Sbjct: 770 LILNP*EGSMAAVAVPLSPYSTKAIPGXASIIRTPLNPGYWQKNICSIKAVASCGSSWTK 591
Query: 609 KMLFGTIISGIMTLCPPAIGIWVSCGAP*TPG*AVTPMP 493
++LFGTIISGIMT P G S GAP* PG*A P P
Sbjct: 590 RILFGTIISGIMTFGPAPKGS*ESGGAP*APG*ACMPTP 474
Score = 105 bits (224), Expect(2) = 3e-083
Identities = 45/104 (43%), Positives = 56/104 (53%)
Frame = +1 / +3
Query: 481 C*SNWHGCNCLPWCLWGSTAHPDTYSRRTKGHDARDYRTKQHLVCPKLATRXXXXXXXXX 660
C* W G CL WCLWGS A T+ T+GHDARDY TKQ+ + P+ TR
Sbjct: 462 C*PGWRGHACLSWCLWGSAALSATFWCGTEGHDARDYCTKQYSLRPRTPTRRDRFDAADV 641
Query: 661 XXXVPWLQGGSDDRGEAWDCFRGVWRRDPGHCCHEQPSKLQNYK 792
VP +Q +DDR AW CF GVWR+ H ++QN +
Sbjct: 642 LLPVPRVQRSADDRSXAWYCFCGVWRQGNSDRSH*TFLRVQNQR 773
Score = 35.4 bits (71), Expect(3) = 6e-044
Identities = 18/32 (56%), Positives = 19/32 (59%)
Frame = +3 / +2
Query: 525 MGLHSSPRYL*QEDKGS*CQRLSYQTTSCLSK 620
MGL S YL D+ S*CQRL YQT SK
Sbjct: 506 MGLRRSLSYLLVRDRRS*CQRLLYQTIFSSSK 601
Score = 30.4 bits (60), Expect(2) = 5e-038
Identities = 16/33 (48%), Positives = 20/33 (60%)
Frame = -3 / -3
Query: 791 L*F*SFEGCSWQQWPGSRLHTPRKQSQASPRSS 693
L*F*+ + W + LHTP+KQ QA RSS
Sbjct: 772 L*F*TLKKVQWLRSLFPCLHTPQKQYQAXLRSS 674
Score = 23.5 bits (45), Expect(3) = 6e-044
Identities = 10/23 (43%), Positives = 13/23 (56%)
Frame = +3 / +2
Query: 726 WSMETRPRPLLP*TTFKASKLQK 794
WSMETR + P K SK ++
Sbjct: 707 WSMETREQRPQPLNLLKGSKSKR 775
>gb|BU969261.1|BU969261 HB11A08r BC Hordeum vulgare subsp. vulgare cDNA clone HB11A08
5-PRIME, mRNA sequence
Length = 545
Score = 210 bits (454), Expect(2) = 9e-078
Identities = 85/102 (83%), Positives = 93/102 (91%)
Frame = +2 / +2
Query: 194 AVFSQFGKILDVLAFKTLKHKGQAWVVFEDVASATEALKRMQGFPFYDKTMRIQYAKTKS 373
AVFSQFGKI++VLAFKTLKHKGQAWVVFEDVASATEALKRMQ FPFYDK M+IQYAKTKS
Sbjct: 11 AVFSQFGKIIEVLAFKTLKHKGQAWVVFEDVASATEALKRMQDFPFYDKPMKIQYAKTKS 190
Query: 374 DIIAKADGTFVPRERRKRTDEKSEKKQKREHHHDAGASQIGM 499
D++AKADGTFVPRE+RKR DEK EKKQKRE HHD + +GM
Sbjct: 191 DVVAKADGTFVPREKRKRNDEKPEKKQKREQHHDVNQAGVGM 316
Score = 169 bits (363), Expect = 3e-041
Identities = 67/106 (63%), Positives = 80/106 (75%)
Frame = +2 / +2
Query: 416 RRKRTDEKSEKKQKREHHHDAGASQIGMGVTAYPGVYGAPQLTQIPIAGGQRVMMPEIIV 595
R KR + ++K++ +Q G+G+ AYPG YGAP L+Q+P G +VMMPEIIV
Sbjct: 227 REKRKRNDEKPEKKQKREQHHDVNQAGVGMHAYPGAYGAPPLSQLPFGAGPKVMMPEIIV 406
Query: 596 PNNILFVQNLPHETTPMMLQMLFCQYPGFKEVRMIEAKPGIAFVEY 733
PNNILFVQ LPH+ T +MLQM FCQYPGFKEVRMIEAKPGIAFVEY
Sbjct: 407 PNNILFVQELPHDATALMLQMFFCQYPGFKEVRMIEAKPGIAFVEY 544
Score = 154 bits (331), Expect(2) = 8e-069
Identities = 65/96 (67%), Positives = 77/96 (80%)
Frame = -1 / -2
Query: 499 HANLTSTSIMVMLALLLLFRFFISPLSSFSRYKGAISLCYNI*FGLGILNSHSFVIEGKA 320
HA+ + +I+V+L LLLLFRF I PL FSRY+GAIS CYNI* GLGILN H FVIEG+
Sbjct: 316 HAHASLVNIVVLLTLLLLFRFLIIPLPFFSRYEGAISFCYNI*LGLGILNFHGFVIEGEI 137
Query: 319 LHPLQSLSS*CDIFEDNPSLTFMLQSLESKHIKNFP 212
LHPLQSL *C+IFE +P L FMLQSL+SKH+ +FP
Sbjct: 136 LHPLQSLGC*CNIFEHDPCLPFMLQSLKSKHLNDFP 29
Score = 138 bits (297), Expect(2) = 8e-037
Identities = 65/93 (69%), Positives = 73/93 (78%)
Frame = +3 / +3
Query: 198 FFPSLGKFLMCLLSRL*SIKVKLGLSSKMSHQLLRL*RGCRAFPSMTKL*EFNMPRPNQI 377
F PSLGK L CLL RL*SIK K G SKM HQ RL*RGC+ PSMT *+FNMPRP+Q+
Sbjct: 15 FSPSLGKSLRCLLLRL*SIKGKHGSCSKMLHQQPRL*RGCKISPSMTNP*KFNMPRPSQM 194
Query: 378 L*QRLMAPLYLEKEERGLMKNLKRSRSASITMM 476
L*Q+LMAP YLEK+ RG+M+NLKRSRS S T M
Sbjct: 195 L*QKLMAPSYLEKKGRGMMRNLKRSRSVSSTTM 293
Score = 138 bits (296), Expect(2) = 5e-064
Identities = 62/74 (83%), Positives = 65/74 (87%)
Frame = -2 / -3
Query: 411 RGTKVPSAFAIISDLVLAY*ILIVLS*KGKPCILFRASVADATSSKTTQA*PLCFKVLKA 232
RGTKVPSAFA SDLVLAY*I + LS*KGK CILF+ASVADATSS TT A PLCFKVLKA
Sbjct: 228 RGTKVPSAFATTSDLVLAY*IFMGLS*KGKSCILFKASVADATSSNTTHACPLCFKVLKA 49
Query: 231 STSRIFPNWEKTAV 190
STS IFPNWEKTA+
Sbjct: 48 STSMIFPNWEKTAL 7
Score = 127 bits (271), Expect(2) = 5e-064
Identities = 52/106 (49%), Positives = 70/106 (66%)
Frame = -1 / -2
Query: 733 ILHESNPRLRLDHPNLLEARVLAEEHLEHHGRRLVWQVLDKQDVVWYDNLWHHDPLSSCY 554
ILH+SN RL DHP+ LE VLAEEHL+H R+VW+ LD++++VWY+NLWHHD S
Sbjct: 544 ILHKSNTRLCFDHPHFLEPWVLAEEHLQHQSGRVVWEFLDEENIVWYNNLWHHDLRSRTK 365
Query: 553 RYLGELWSPINTRVSSYTHANLTSTSIMVMLALLLLFRFFISPLSS 416
R L E SPI+TR+ + HA+L + +++ L LL F P S
Sbjct: 364 R*LRERRSPISTRIGMHAHASLVNIVVLLTLLLLFRFLIIPLPFFS 227
Score = 127 bits (271), Expect(2) = 8e-069
Identities = 56/80 (70%), Positives = 59/80 (73%)
Frame = -2 / -3
Query: 732 YSTKAIPGFASIIRTSLKPGYWQKSIWSIMGVVSCGKFWTNKMLFGTIISGIMTLCPPAI 553
YSTKAIPGFASIIRTSL PGYWQK+I SI V SCG WT ++LFGTIISGIMT P
Sbjct: 543 YSTKAIPGFASIIRTSLNPGYWQKNICSIKAVASCGSSWTKRILFGTIISGIMTFGPAPK 364
Query: 552 GIWVSCGAP*TPG*AVTPMP 493
G S GAP* PG*A P P
Sbjct: 363 GS*ESGGAP*APG*ACMPTP 304
Score = 124 bits (266), Expect = 7e-028
Identities = 58/108 (53%), Positives = 68/108 (62%)
Frame = -3 / -1
Query: 521 HQGKQLHPCQFD*HQHHGDARXXXXXXXXXXXXXXFLEVQRCHQPLL*YLIWSWHIEFS* 342
H+ + H C H G A FLEV+RCHQ LL +L WSWHIEFS
Sbjct: 338 HKHQDRHACPRQPG*HRGAAHASASFQVSHHSSSFFLEVRRCHQLLLQHLTWSWHIEFSW 159
Query: 341 FCHRRESPASSSEPQ*LMRHLRRQPKLDLYASKS*KQAHQEFSQTGKK 198
CHRR + ASSS+P+ LM+HLR +P L LYASKS*KQA Q FSQTG+K
Sbjct: 158 VCHRRGNLASSSKPRLLMQHLRTRPMLALYASKS*KQAPQ*FSQTGRK 15
Score = 115 bits (245), Expect = 6e-025
Identities = 57/93 (61%), Positives = 63/93 (67%)
Frame = +1 / +1
Query: 199 FFPVWENS*CACFQDFEAXXXXXXXXXXXXISY*GSEEDAGLSLL*QNYENSICQDQIRY 378
F PVWEN * ACF DFEA IS G EEDA LL*Q +ENSICQDQ+R
Sbjct: 16 FLPVWENH*GACF*DFEA*RASMGRVRRCCISNRGFEEDARFPLL*QTHENSICQDQVRC 195
Query: 379 YSKG*WHLCTSRKKKED**KI*KEAEARASP*C 477
SK *WHL TSRKK+E+**+ *KEAEA A+P C
Sbjct: 196 CSKS*WHLRTSRKKEEE**ET*KEAEA*AAPRC 294
Score = 100 bits (213), Expect(2) = 9e-078
Identities = 41/84 (48%), Positives = 49/84 (58%)
Frame = +1 / +1
Query: 481 C*SNWHGCNCLPWCLWGSTAHPDTYSRRTKGHDARDYRTKQHLVCPKLATRXXXXXXXXX 660
C* W G CL WCLWGS A T+ T+GHDARDY TKQ+ + P+ TR
Sbjct: 292 C*PGWRGHACLSWCLWGSAALSATFWCGTEGHDARDYCTKQYSLRPRTPTRRDRFDAADV 471
Query: 661 XXXVPWLQGGSDDRGEAWDCFRGV 732
VP +QG +DDR +AW CF GV
Sbjct: 472 LLPVPRVQGSADDRSKAWYCFCGV 543
Score = 35.4 bits (71), Expect(2) = 8e-037
Identities = 18/32 (56%), Positives = 19/32 (59%)
Frame = +3 / +3
Query: 525 MGLHSSPRYL*QEDKGS*CQRLSYQTTSCLSK 620
MGL S YL D+ S*CQRL YQT SK
Sbjct: 336 MGLRRSLSYLLVRDRRS*CQRLLYQTIFSSSK 431
>gb|BJ547861.1|BJ547861 BJ547861 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal40p14 5', mRNA sequence
Length = 604
Score = 224 bits (483), Expect(2) = 3e-073
Identities = 93/128 (72%), Positives = 102/128 (79%)
Frame = +2 / +2
Query: 116 IPPNVTIYXXXXXXXXXXXXXXXSLTAVFSQFGKILDVLAFKTLKHKGQAWVVFEDVASA 295
+ PNVTIY SL AVFSQFGKI++VLAFKTLKHKGQAWVVFEDVASA
Sbjct: 56 VQPNVTIYINNLNEKIKLDALKKSLNAVFSQFGKIIEVLAFKTLKHKGQAWVVFEDVASA 235
Query: 296 TEALKRMQGFPFYDKTMRIQYAKTKSDIIAKADGTFVPRERRKRTDEKSEKKQKREHHHD 475
TEALKRMQ FPFYDK M+IQYAKTKSD++AKADGTFVPRE+RKR DEK EKKQKRE HHD
Sbjct: 236 TEALKRMQDFPFYDKPMKIQYAKTKSDVVAKADGTFVPREKRKRNDEKPEKKQKREQHHD 415
Query: 476 AGASQIGM 499
+ +GM
Sbjct: 416 VNQAGVGM 439
Score = 183 bits (394), Expect(2) = 2e-063
Identities = 82/136 (60%), Positives = 98/136 (72%)
Frame = -1 / -1
Query: 499 HANLTSTSIMVMLALLLLFRFFISPLSSFSRYKGAISLCYNI*FGLGILNSHSFVIEGKA 320
HA+ + +I+V+L LLLLFRF I PL FSRY+GAIS CYNI* GLGILN H FVIEG+
Sbjct: 439 HAHASLVNIVVLLTLLLLFRFLIIPLPFFSRYEGAISFCYNI*LGLGILNFHGFVIEGEI 260
Query: 319 LHPLQSLSS*CDIFEDNPSLTFMLQSLESKHIKNFPXXXXXXXXXXXKFFELDLLVEVVD 140
LHPLQSL *C+IFE +P L FMLQSL+SKH+ +FP K E DLLVEV+D
Sbjct: 259 LHPLQSLGC*CNIFEHDPCLPFMLQSLKSKHLNDFPKLGENRIEGLLKCVEFDLLVEVID 80
Query: 139 IDGDVRRDAIRLSFAS 92
+DG V DA+RL A+
Sbjct: 79 VDGHVWLDAVRLPAAA 32
Score = 157 bits (338), Expect(2) = 2e-042
Identities = 76/120 (63%), Positives = 84/120 (70%)
Frame = +1 / +1
Query: 118 PAERHHLYQQPQREDQARRT*EVPNSRFFPVWENS*CACFQDFEAXXXXXXXXXXXXISY 297
PA+R HL+Q PQREDQ RRT*EVP F PVWEN * ACF DFEA IS
Sbjct: 58 PAKRDHLHQ*PQREDQTRRT*EVPQCGFLPVWENH*GACF*DFEA*RASMGRVRRCCISN 237
Query: 298 *GSEEDAGLSLL*QNYENSICQDQIRYYSKG*WHLCTSRKKKED**KI*KEAEARASP*C 477
G EEDA LL*Q +ENSICQDQ+R SK *WHL TSRKK+E+**+ *KEAEA A+P C
Sbjct: 238 RGFEEDARFPLL*QTHENSICQDQVRCCSKS*WHLRTSRKKEEE**ET*KEAEA*AAPRC 417
Score = 150 bits (323), Expect = 1e-035
Identities = 76/135 (56%), Positives = 86/135 (63%)
Frame = -3 / -3
Query: 521 HQGKQLHPCQFD*HQHHGDARXXXXXXXXXXXXXXFLEVQRCHQPLL*YLIWSWHIEFS* 342
H+ + H C H G A FLEV+RCHQ LL +L WSWHIEFS
Sbjct: 461 HKHQDRHACPRQPG*HRGAAHASASFQVSHHSSSFFLEVRRCHQLLLQHLTWSWHIEFSW 282
Query: 341 FCHRRESPASSSEPQ*LMRHLRRQPKLDLYASKS*KQAHQEFSQTGKKRLLGTS*VLRA* 162
CHRR + ASSS+P+ LM+HLR +P L LYASKS*KQA Q FSQTG+K GTS*V R *
Sbjct: 281 VCHRRGNLASSSKPRLLMQHLRTRPMLALYASKS*KQAPQ*FSQTGRKPH*GTS*VRRV* 102
Query: 161 SSR*GC*YRW*RSAG 117
SSR*G * RW R AG
Sbjct: 101 SSR*GY*CRWSRLAG 57
Score = 149 bits (319), Expect(2) = 1e-058
Identities = 70/97 (72%), Positives = 73/97 (75%)
Frame = -2 / -2
Query: 411 RGTKVPSAFAIISDLVLAY*ILIVLS*KGKPCILFRASVADATSSKTTQA*PLCFKVLKA 232
RGTKVPSAFA SDLVLAY*I + LS*KGK CILF+ASVADATSS TT A PLCFKVLKA
Sbjct: 351 RGTKVPSAFATTSDLVLAY*IFMGLS*KGKSCILFKASVADATSSNTTHACPLCFKVLKA 172
Query: 231 STSRIFPNWEKTAVRDXXXXXXXXXXXXXXX*MVTFG 121
STS IFPNWEKTA+RD *MVTFG
Sbjct: 171 STSMIFPNWEKTALRDFLSASSLIFSLRLLM*MVTFG 61
Score = 146 bits (313), Expect = 2e-034
Identities = 72/124 (58%), Positives = 81/124 (65%)
Frame = +3 / +3
Query: 105 RRMAXXXXXXXXXXXXXXXXXXKNLRSP*QPFFPSLGKFLMCLLSRL*SIKVKLGLSSKM 284
RR A +LRSP F PSLGK L CLL RL*SIK K G SKM
Sbjct: 45 RRTASSQT*PSTSITSTRRSNSTHLRSPSMRFSPSLGKSLRCLLLRL*SIKGKHGSCSKM 224
Query: 285 SHQLLRL*RGCRAFPSMTKL*EFNMPRPNQIL*QRLMAPLYLEKEERGLMKNLKRSRSAS 464
HQ RL*RGC+ PSMT *+FNMPRP+Q+L*Q+LMAP YLEK+ RG+M+NLKRSRS S
Sbjct: 225 LHQQPRL*RGCKISPSMTNP*KFNMPRPSQML*QKLMAPSYLEKKGRGMMRNLKRSRSVS 404
Query: 465 ITMM 476
T M
Sbjct: 405 STTM 416
Score = 118 bits (252), Expect = 6e-026
Identities = 46/85 (54%), Positives = 59/85 (69%)
Frame = +2 / +2
Query: 416 RRKRTDEKSEKKQKREHHHDAGASQIGMGVTAYPGVYGAPQLTQIPIAGGQRVMMPEIIV 595
R KR + ++K++ +Q G+G+ AYPG YGAP L+Q+P G +VMMPEIIV
Sbjct: 350 REKRKRNDEKPEKKQKREQHHDVNQAGVGMHAYPGAYGAPPLSQLPFGAGPKVMMPEIIV 529
Query: 596 PNNILFVQNLPHETTPMMLQMLFCQ 670
PNNILFVQ LPH+ T +MLQM FCQ
Sbjct: 530 PNNILFVQELPHDATALMLQMFFCQ 604
Score = 98.2 bits (208), Expect(2) = 1e-058
Identities = 39/85 (45%), Positives = 55/85 (64%)
Frame = -1 / -1
Query: 670 LAEEHLEHHGRRLVWQVLDKQDVVWYDNLWHHDPLSSCYRYLGELWSPINTRVSSYTHAN 491
LAEEHL+H R+VW+ LD++++VWY+NLWHHD S R L E SPI+TR+ + HA+
Sbjct: 604 LAEEHLQHQSGRVVWEFLDEENIVWYNNLWHHDLRSRTKR*LRERRSPISTRIGMHAHAS 425
Query: 490 LTSTSIMVMLALLLLFRFFISPLSS 416
L + +++ L LL F P S
Sbjct: 424 LVNIVVLLTLLLLFRFLIIPLPFFS 350
Score = 80.3 bits (169), Expect(2) = 2e-063
Identities = 36/59 (61%), Positives = 39/59 (66%)
Frame = -2 / -2
Query: 669 WQKSIWSIMGVVSCGKFWTNKMLFGTIISGIMTLCPPAIGIWVSCGAP*TPG*AVTPMP 493
WQK+I SI V SCG WT ++LFGTIISGIMT P G S GAP* PG*A P P
Sbjct: 603 WQKNICSIKAVASCGSSWTKRILFGTIISGIMTFGPAPKGS*ESGGAP*APG*ACMPTP 427
Score = 72.1 bits (151), Expect(2) = 3e-073
Identities = 28/51 (54%), Positives = 33/51 (64%)
Frame = +1 / +1
Query: 481 C*SNWHGCNCLPWCLWGSTAHPDTYSRRTKGHDARDYRTKQHLVCPKLATR 633
C* W G CL WCLWGS A T+ T+GHDARDY TKQ+ + P+ TR
Sbjct: 415 C*PGWRGHACLSWCLWGSAALSATFWCGTEGHDARDYCTKQYSLRPRTPTR 567
Score = 35.4 bits (71), Expect(2) = 2e-042
Identities = 18/32 (56%), Positives = 19/32 (59%)
Frame = +3 / +3
Query: 525 MGLHSSPRYL*QEDKGS*CQRLSYQTTSCLSK 620
MGL S YL D+ S*CQRL YQT SK
Sbjct: 459 MGLRRSLSYLLVRDRRS*CQRLLYQTIFSSSK 554
>gb|BJ547531.1|BJ547531 BJ547531 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal28m19 5', mRNA sequence
Length = 563
Score = 224 bits (483), Expect(2) = 7e-072
Identities = 93/128 (72%), Positives = 102/128 (79%)
Frame = +2 / +1
Query: 116 IPPNVTIYXXXXXXXXXXXXXXXSLTAVFSQFGKILDVLAFKTLKHKGQAWVVFEDVASA 295
+ PNVTIY SL AVFSQFGKI++VLAFKTLKHKGQAWVVFEDVASA
Sbjct: 67 VQPNVTIYINNLNEKIKLDALKKSLNAVFSQFGKIIEVLAFKTLKHKGQAWVVFEDVASA 246
Query: 296 TEALKRMQGFPFYDKTMRIQYAKTKSDIIAKADGTFVPRERRKRTDEKSEKKQKREHHHD 475
TEALKRMQ FPFYDK M+IQYAKTKSD++AKADGTFVPRE+RKR DEK EKKQKRE HHD
Sbjct: 247 TEALKRMQDFPFYDKPMKIQYAKTKSDVVAKADGTFVPREKRKRNDEKPEKKQKREQHHD 426
Query: 476 AGASQIGM 499
+ +GM
Sbjct: 427 VNQAGVGM 450
Score = 183 bits (394), Expect(2) = 2e-056
Identities = 82/136 (60%), Positives = 98/136 (72%)
Frame = -1 / -3
Query: 499 HANLTSTSIMVMLALLLLFRFFISPLSSFSRYKGAISLCYNI*FGLGILNSHSFVIEGKA 320
HA+ + +I+V+L LLLLFRF I PL FSRY+GAIS CYNI* GLGILN H FVIEG+
Sbjct: 450 HAHASLVNIVVLLTLLLLFRFLIIPLPFFSRYEGAISFCYNI*LGLGILNFHGFVIEGEI 271
Query: 319 LHPLQSLSS*CDIFEDNPSLTFMLQSLESKHIKNFPXXXXXXXXXXXKFFELDLLVEVVD 140
LHPLQSL *C+IFE +P L FMLQSL+SKH+ +FP K E DLLVEV+D
Sbjct: 270 LHPLQSLGC*CNIFEHDPCLPFMLQSLKSKHLNDFPKLGENRIEGLLKCVEFDLLVEVID 91
Query: 139 IDGDVRRDAIRLSFAS 92
+DG V DA+RL A+
Sbjct: 90 VDGHVWLDAVRLPAAA 43
Score = 157 bits (338), Expect(2) = 5e-042
Identities = 76/120 (63%), Positives = 84/120 (70%)
Frame = +1 / +3
Query: 118 PAERHHLYQQPQREDQARRT*EVPNSRFFPVWENS*CACFQDFEAXXXXXXXXXXXXISY 297
PA+R HL+Q PQREDQ RRT*EVP F PVWEN * ACF DFEA IS
Sbjct: 69 PAKRDHLHQ*PQREDQTRRT*EVPQCGFLPVWENH*GACF*DFEA*RASMGRVRRCCISN 248
Query: 298 *GSEEDAGLSLL*QNYENSICQDQIRYYSKG*WHLCTSRKKKED**KI*KEAEARASP*C 477
G EEDA LL*Q +ENSICQDQ+R SK *WHL TSRKK+E+**+ *KEAEA A+P C
Sbjct: 249 RGFEEDARFPLL*QTHENSICQDQVRCCSKS*WHLRTSRKKEEE**ET*KEAEA*AAPRC 428
Score = 150 bits (323), Expect = 1e-035
Identities = 76/135 (56%), Positives = 86/135 (63%)
Frame = -3 / -2
Query: 521 HQGKQLHPCQFD*HQHHGDARXXXXXXXXXXXXXXFLEVQRCHQPLL*YLIWSWHIEFS* 342
H+ + H C H G A FLEV+RCHQ LL +L WSWHIEFS
Sbjct: 472 HKHQDRHACPRQPG*HRGAAHASASFQVSHHSSSFFLEVRRCHQLLLQHLTWSWHIEFSW 293
Query: 341 FCHRRESPASSSEPQ*LMRHLRRQPKLDLYASKS*KQAHQEFSQTGKKRLLGTS*VLRA* 162
CHRR + ASSS+P+ LM+HLR +P L LYASKS*KQA Q FSQTG+K GTS*V R *
Sbjct: 292 VCHRRGNLASSSKPRLLMQHLRTRPMLALYASKS*KQAPQ*FSQTGRKPH*GTS*VRRV* 113
Query: 161 SSR*GC*YRW*RSAG 117
SSR*G * RW R AG
Sbjct: 112 SSR*GY*CRWSRLAG 68
Score = 149 bits (319), Expect(2) = 8e-050
Identities = 70/97 (72%), Positives = 73/97 (75%)
Frame = -2 / -1
Query: 411 RGTKVPSAFAIISDLVLAY*ILIVLS*KGKPCILFRASVADATSSKTTQA*PLCFKVLKA 232
RGTKVPSAFA SDLVLAY*I + LS*KGK CILF+ASVADATSS TT A PLCFKVLKA
Sbjct: 362 RGTKVPSAFATTSDLVLAY*IFMGLS*KGKSCILFKASVADATSSNTTHACPLCFKVLKA 183
Query: 231 STSRIFPNWEKTAVRDXXXXXXXXXXXXXXX*MVTFG 121
STS IFPNWEKTA+RD *MVTFG
Sbjct: 182 STSMIFPNWEKTALRDFLSASSLIFSLRLLM*MVTFG 72
Score = 146 bits (313), Expect = 2e-034
Identities = 72/124 (58%), Positives = 81/124 (65%)
Frame = +3 / +2
Query: 105 RRMAXXXXXXXXXXXXXXXXXXKNLRSP*QPFFPSLGKFLMCLLSRL*SIKVKLGLSSKM 284
RR A +LRSP F PSLGK L CLL RL*SIK K G SKM
Sbjct: 56 RRTASSQT*PSTSITSTRRSNSTHLRSPSMRFSPSLGKSLRCLLLRL*SIKGKHGSCSKM 235
Query: 285 SHQLLRL*RGCRAFPSMTKL*EFNMPRPNQIL*QRLMAPLYLEKEERGLMKNLKRSRSAS 464
HQ RL*RGC+ PSMT *+FNMPRP+Q+L*Q+LMAP YLEK+ RG+M+NLKRSRS S
Sbjct: 236 LHQQPRL*RGCKISPSMTNP*KFNMPRPSQML*QKLMAPSYLEKKGRGMMRNLKRSRSVS 415
Query: 465 ITMM 476
T M
Sbjct: 416 STTM 427
Score = 68.9 bits (144), Expect(2) = 8e-050
Identities = 28/67 (41%), Positives = 41/67 (61%)
Frame = -1 / -3
Query: 616 DKQDVVWYDNLWHHDPLSSCYRYLGELWSPINTRVSSYTHANLTSTSIMVMLALLLLFRF 437
D++++VWY+NLWHHD S R L E SPI+TR+ + HA+L + +++ L LL F
Sbjct: 561 DEENIVWYNNLWHHDLRSRTKR*LRERRSPISTRIGMHAHASLVNIVVLLTLLLLFRFLI 382
Query: 436 FISPLSS 416
P S
Sbjct: 381 IPLPFFS 361
Score = 67.5 bits (141), Expect(2) = 7e-072
Identities = 26/46 (56%), Positives = 30/46 (65%)
Frame = +1 / +3
Query: 481 C*SNWHGCNCLPWCLWGSTAHPDTYSRRTKGHDARDYRTKQHLVCP 618
C* W G CL WCLWGS A T+ T+GHDARDY TKQ+ + P
Sbjct: 426 C*PGWRGHACLSWCLWGSAALSATFWCGTEGHDARDYCTKQYSLRP 563
Score = 56.5 bits (117), Expect(2) = 2e-056
Identities = 26/42 (61%), Positives = 28/42 (66%)
Frame = -2 / -1
Query: 618 WTNKMLFGTIISGIMTLCPPAIGIWVSCGAP*TPG*AVTPMP 493
WT ++LFGTIISGIMT P G S GAP* PG*A P P
Sbjct: 563 WTKRILFGTIISGIMTFGPAPKGS*ESGGAP*APG*ACMPTP 438
Score = 34.1 bits (68), Expect(2) = 5e-042
Identities = 16/26 (61%), Positives = 17/26 (65%)
Frame = +3 / +2
Query: 525 MGLHSSPRYL*QEDKGS*CQRLSYQT 602
MGL S YL D+ S*CQRL YQT
Sbjct: 470 MGLRRSLSYLLVRDRRS*CQRLLYQT 547
>gb|BJ548991.1|BJ548991 BJ548991 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal40p14 3', mRNA sequence
Length = 612
Score = 200 bits (432), Expect(3) = 3e-055
Identities = 82/124 (66%), Positives = 95/124 (76%)
Frame = +2 / -3
Query: 416 RRKRTDEKSEKKQKREHHHDAGASQIGMGVTAYPGVYGAPQLTQIPIAGGQRVMMPEIIV 595
R KR + ++K++ +Q G+G+ AYPG YGAP L+Q+P G +VMMPEIIV
Sbjct: 514 REKRKRNDEKPEKKQKREQHHDVNQAGVGMHAYPGAYGAPPLSQLPFGAGPKVMMPEIIV 335
Query: 596 PNNILFVQNLPHETTPMMLQMLFCQYPGFKEVRMIEAKPGIAFVEYGDETQATAAMNNLQ 775
PNNILFVQ LPH+ T +MLQM FCQYPGFKEVRMIEAKPGIAFVEYGDE QATAAMN LQ
Sbjct: 334 PNNILFVQELPHDATALMLQMFFCQYPGFKEVRMIEAKPGIAFVEYGDEGQATAAMNLLQ 155
Query: 776 SFKI 787
FKI
Sbjct: 154 GFKI 143
Score = 151 bits (324), Expect(4) = 7e-067
Identities = 70/99 (70%), Positives = 74/99 (74%)
Frame = -2 / +3
Query: 789 VILKL*RLFMAAVAWVSSPYSTKAIPGFASIIRTSLKPGYWQKSIWSIMGVVSCGKFWTN 610
+ILK *R FMAAVA SSPYSTKAIPGFASIIRTSL PGYWQK+I SI V SCG WT
Sbjct: 141 LILKP*RRFMAAVACPSSPYSTKAIPGFASIIRTSLNPGYWQKNICSIKAVASCGSSWTK 320
Query: 609 KMLFGTIISGIMTLCPPAIGIWVSCGAP*TPG*AVTPMP 493
++LFGTIISGIMT P G S GAP* PG*A P P
Sbjct: 321 RILFGTIISGIMTFGPAPKGS*ESGGAP*APG*ACMPTP 437
Score = 149 bits (321), Expect(3) = 1e-050
Identities = 63/124 (50%), Positives = 82/124 (66%)
Frame = -1 / +2
Query: 787 NFEALKVVHGSSGLGLVSILHESNPRLRLDHPNLLEARVLAEEHLEHHGRRLVWQVLDKQ 608
+FE LK VHG L LVSILH+SN RL DHP+ LE VLAEEHL+H R+VW+ LD++
Sbjct: 143 DFETLKKVHGCGRLSLVSILHKSNTRLCFDHPHFLEPWVLAEEHLQHQSGRVVWEFLDEE 322
Query: 607 DVVWYDNLWHHDPLSSCYRYLGELWSPINTRVSSYTHANLTSTSIMVMLALLLLFRFFIS 428
++VWY+NLWHHD S R L E SPI+TR+ + HA+L + +++ L LL F
Sbjct: 323 NIVWYNNLWHHDLRSRTKR*LRERRSPISTRIGMHAHASLVNIVVLLTLLLLFRFLIIPL 502
Query: 427 PLSS 416
P S
Sbjct: 503 PFFS 514
Score = 122 bits (261), Expect(3) = 1e-062
Identities = 47/62 (75%), Positives = 53/62 (85%)
Frame = +2 / -3
Query: 314 MQGFPFYDKTMRIQYAKTKSDIIAKADGTFVPRERRKRTDEKSEKKQKREHHHDAGASQI 493
MQ FPFYDK M+IQYAKTKSD++AKADGTFVPRE+RKR DEK EKKQKRE HHD + +
Sbjct: 610 MQDFPFYDKPMKIQYAKTKSDVVAKADGTFVPREKRKRNDEKPEKKQKREQHHDVNQAGV 431
Query: 494 GM 499
GM
Sbjct: 430 GM 425
Score = 121 bits (259), Expect(3) = 1e-062
Identities = 51/110 (46%), Positives = 60/110 (54%)
Frame = +1 / -2
Query: 481 C*SNWHGCNCLPWCLWGSTAHPDTYSRRTKGHDARDYRTKQHLVCPKLATRXXXXXXXXX 660
C* W G CL WCLWGS A T+ T+GHDARDY TKQ+ + P+ TR
Sbjct: 449 C*PGWRGHACLSWCLWGSAALSATFWCGTEGHDARDYCTKQYSLRPRTPTRRDRFDAADV 270
Query: 661 XXXVPWLQGGSDDRGEAWDCFRGVWRRDPGHCCHEQPSKLQNYKRKPDGH 810
VP +QG +DDR +AW CF GVWRR G HE S QN + H
Sbjct: 269 LLPVPRVQGSADDRSKAWYCFCGVWRRGTGDRSHEPSSGFQNQREPNAHH 120
Score = 99.6 bits (211), Expect(4) = 7e-067
Identities = 42/62 (67%), Positives = 49/62 (79%)
Frame = -1 / +2
Query: 499 HANLTSTSIMVMLALLLLFRFFISPLSSFSRYKGAISLCYNI*FGLGILNSHSFVIEGKA 320
HA+ + +I+V+L LLLLFRF I PL FSRY+GAIS CYNI* GLGILN H FVIEG+
Sbjct: 425 HAHASLVNIVVLLTLLLLFRFLIIPLPFFSRYEGAISFCYNI*LGLGILNFHGFVIEGEI 604
Query: 319 LH 314
LH
Sbjct: 605 LH 610
Score = 90.4 bits (191), Expect(4) = 2e-028
Identities = 38/55 (69%), Positives = 46/55 (83%)
Frame = +3 / -1
Query: 312 GCRAFPSMTKL*EFNMPRPNQIL*QRLMAPLYLEKEERGLMKNLKRSRSASITMM 476
GC+ PSMT *+FNMPRP+Q+L*Q+LMAP YLEK+ RG+M+NLKRSRS S T M
Sbjct: 612 GCKISPSMTNP*KFNMPRPSQML*QKLMAPSYLEKKGRGMMRNLKRSRSVSSTTM 448
Score = 58.3 bits (121), Expect(3) = 1e-050
Identities = 27/33 (81%), Positives = 28/33 (84%)
Frame = -2 / +3
Query: 411 RGTKVPSAFAIISDLVLAY*ILIVLS*KGKPCI 313
RGTKVPSAFA SDLVLAY*I + LS*KGK CI
Sbjct: 513 RGTKVPSAFATTSDLVLAY*IFMGLS*KGKSCI 611
Score = 35.4 bits (71), Expect(3) = 1e-062
Identities = 13/25 (52%), Positives = 15/25 (60%)
Frame = +1 / -1
Query: 865 CCIPSFTQLVNFLHVLGRQHPEQPV 939
CC P F V+FLH+ GR H PV
Sbjct: 75 CCSPGFAPSVDFLHM*GRLHQVNPV 1
Score = 35.4 bits (71), Expect(4) = 2e-028
Identities = 18/32 (56%), Positives = 19/32 (59%)
Frame = +3 / -1
Query: 525 MGLHSSPRYL*QEDKGS*CQRLSYQTTSCLSK 620
MGL S YL D+ S*CQRL YQT SK
Sbjct: 405 MGLRRSLSYLLVRDRRS*CQRLLYQTIFSSSK 310
Score = 31.3 bits (62), Expect(3) = 3e-055
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +1 / -2
Query: 766 QPSKLQNYKRKPDGHHICEEV 828
+PS +R+P+ HHICEEV
Sbjct: 167 EPSSGFQNQREPNAHHICEEV 105
Score = 30.9 bits (61), Expect(4) = 2e-028
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = +3 / -1
Query: 726 WSMETRPRPLLP*TTFKASKLQK 794
WSMETR R P*T F+ SK ++
Sbjct: 204 WSMETRDRRPQP*TFFRVSKSKR 136
Score = 30.9 bits (61), Expect(4) = 7e-067
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = -2 / +3
Query: 876 RNTTSHTSGKHCSGAHYFFAYVMTIWFSFVI 784
+N + + S + YFFAYVM I FS ++
Sbjct: 57 QNXGYNINSSQYSSSSYFFAYVMRIGFSLIL 149
Score = 30.9 bits (61), Expect(3) = 1e-050
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = -3 / +2
Query: 938 TGCSGCCLPNT*RKFTSCVKLGIQHLTHQVNIARELI 828
TG + C LP *RK T K G+QH + + + L+
Sbjct: 2 TGLTWCSLPYM*RKSTDGAKPGLQH*FKSIQLIKLLL 112
Score = 30.4 bits (60), Expect(4) = 7e-067
Identities = 13/20 (65%), Positives = 15/20 (75%)
Frame = -1 / +3
Query: 919 VYLTHEGNLPVV*NSEYNIS 860
VYLT E NLP+V N YNI+
Sbjct: 21 VYLTCEENLPMVQNXGYNIN 80
Score = 24.9 bits (48), Expect(4) = 2e-028
Identities = 11/13 (84%), Positives = 11/13 (84%)
Frame = +2 / -3
Query: 791 KENQMVITYAKK* 829
KEN M ITYAKK*
Sbjct: 142 KENPMRITYAKK* 104
Score = 22.6 bits (43), Expect(3) = 3e-055
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = +2 / -2
Query: 863 DVVFRVLHNW*ISFMC*VD 919
+VV VLH+ *I F C VD
Sbjct: 77 NVVAXVLHHR*IFFTCKVD 21
>gb|BU979069.1|BU979069 HA15B05r HA Hordeum vulgare subsp. vulgare cDNA clone HA15B05
5-PRIME, mRNA sequence
Length = 548
Score = 224 bits (483), Expect(2) = 2e-066
Identities = 93/128 (72%), Positives = 102/128 (79%)
Frame = +2 / +1
Query: 116 IPPNVTIYXXXXXXXXXXXXXXXSLTAVFSQFGKILDVLAFKTLKHKGQAWVVFEDVASA 295
+ PNVTIY SL AVFSQFGKI++VLAFKTLKHKGQAWVVFEDVASA
Sbjct: 91 VQPNVTIYINNLNEKIKLDALKKSLNAVFSQFGKIIEVLAFKTLKHKGQAWVVFEDVASA 270
Query: 296 TEALKRMQGFPFYDKTMRIQYAKTKSDIIAKADGTFVPRERRKRTDEKSEKKQKREHHHD 475
TEALKRMQ FPFYDK M+IQYAKTKSD++AKADGTFVPRE+RKR DEK EKKQKRE HHD
Sbjct: 271 TEALKRMQDFPFYDKPMKIQYAKTKSDVVAKADGTFVPREKRKRNDEKPEKKQKREQHHD 450
Query: 476 AGASQIGM 499
+ +GM
Sbjct: 451 VNQAGVGM 474
Score = 183 bits (394), Expect(2) = 2e-048
Identities = 82/136 (60%), Positives = 98/136 (72%)
Frame = -1 / -3
Query: 499 HANLTSTSIMVMLALLLLFRFFISPLSSFSRYKGAISLCYNI*FGLGILNSHSFVIEGKA 320
HA+ + +I+V+L LLLLFRF I PL FSRY+GAIS CYNI* GLGILN H FVIEG+
Sbjct: 474 HAHASLVNIVVLLTLLLLFRFLIIPLPFFSRYEGAISFCYNI*LGLGILNFHGFVIEGEI 295
Query: 319 LHPLQSLSS*CDIFEDNPSLTFMLQSLESKHIKNFPXXXXXXXXXXXKFFELDLLVEVVD 140
LHPLQSL *C+IFE +P L FMLQSL+SKH+ +FP K E DLLVEV+D
Sbjct: 294 LHPLQSLGC*CNIFEHDPCLPFMLQSLKSKHLNDFPKLGENRIEGLLKCVEFDLLVEVID 115
Query: 139 IDGDVRRDAIRLSFAS 92
+DG V DA+RL A+
Sbjct: 114 VDGHVWLDAVRLPAAA 67
Score = 157 bits (338), Expect = 8e-038
Identities = 76/120 (63%), Positives = 84/120 (70%)
Frame = +1 / +3
Query: 118 PAERHHLYQQPQREDQARRT*EVPNSRFFPVWENS*CACFQDFEAXXXXXXXXXXXXISY 297
PA+R HL+Q PQREDQ RRT*EVP F PVWEN * ACF DFEA IS
Sbjct: 93 PAKRDHLHQ*PQREDQTRRT*EVPQCGFLPVWENH*GACF*DFEA*RASMGRVRRCCISN 272
Query: 298 *GSEEDAGLSLL*QNYENSICQDQIRYYSKG*WHLCTSRKKKED**KI*KEAEARASP*C 477
G EEDA LL*Q +ENSICQDQ+R SK *WHL TSRKK+E+**+ *KEAEA A+P C
Sbjct: 273 RGFEEDARFPLL*QTHENSICQDQVRCCSKS*WHLRTSRKKEEE**ET*KEAEA*AAPRC 452
Score = 150 bits (323), Expect = 1e-035
Identities = 76/135 (56%), Positives = 86/135 (63%)
Frame = -3 / -2
Query: 521 HQGKQLHPCQFD*HQHHGDARXXXXXXXXXXXXXXFLEVQRCHQPLL*YLIWSWHIEFS* 342
H+ + H C H G A FLEV+RCHQ LL +L WSWHIEFS
Sbjct: 496 HKHQDRHACPRQPG*HRGAAHASASFQVSHHSSSFFLEVRRCHQLLLQHLTWSWHIEFSW 317
Query: 341 FCHRRESPASSSEPQ*LMRHLRRQPKLDLYASKS*KQAHQEFSQTGKKRLLGTS*VLRA* 162
CHRR + ASSS+P+ LM+HLR +P L LYASKS*KQA Q FSQTG+K GTS*V R *
Sbjct: 316 VCHRRGNLASSSKPRLLMQHLRTRPMLALYASKS*KQAPQ*FSQTGRKPH*GTS*VRRV* 137
Query: 161 SSR*GC*YRW*RSAG 117
SSR*G * RW R AG
Sbjct: 136 SSR*GY*CRWSRLAG 92
Score = 149 bits (319), Expect(2) = 7e-041
Identities = 70/97 (72%), Positives = 73/97 (75%)
Frame = -2 / -1
Query: 411 RGTKVPSAFAIISDLVLAY*ILIVLS*KGKPCILFRASVADATSSKTTQA*PLCFKVLKA 232
RGTKVPSAFA SDLVLAY*I + LS*KGK CILF+ASVADATSS TT A PLCFKVLKA
Sbjct: 386 RGTKVPSAFATTSDLVLAY*IFMGLS*KGKSCILFKASVADATSSNTTHACPLCFKVLKA 207
Query: 231 STSRIFPNWEKTAVRDXXXXXXXXXXXXXXX*MVTFG 121
STS IFPNWEKTA+RD *MVTFG
Sbjct: 206 STSMIFPNWEKTALRDFLSASSLIFSLRLLM*MVTFG 96
Score = 146 bits (313), Expect = 2e-034
Identities = 72/124 (58%), Positives = 81/124 (65%)
Frame = +3 / +2
Query: 105 RRMAXXXXXXXXXXXXXXXXXXKNLRSP*QPFFPSLGKFLMCLLSRL*SIKVKLGLSSKM 284
RR A +LRSP F PSLGK L CLL RL*SIK K G SKM
Sbjct: 80 RRTASSQT*PSTSITSTRRSNSTHLRSPSMRFSPSLGKSLRCLLLRL*SIKGKHGSCSKM 259
Query: 285 SHQLLRL*RGCRAFPSMTKL*EFNMPRPNQIL*QRLMAPLYLEKEERGLMKNLKRSRSAS 464
HQ RL*RGC+ PSMT *+FNMPRP+Q+L*Q+LMAP YLEK+ RG+M+NLKRSRS S
Sbjct: 260 LHQQPRL*RGCKISPSMTNP*KFNMPRPSQML*QKLMAPSYLEKKGRGMMRNLKRSRSVS 439
Query: 465 ITMM 476
T M
Sbjct: 440 STTM 451
Score = 49.6 bits (102), Expect(2) = 2e-066
Identities = 18/33 (54%), Positives = 20/33 (60%)
Frame = +1 / +3
Query: 481 C*SNWHGCNCLPWCLWGSTAHPDTYSRRTKGHD 579
C* W G CL WCLWGS A T+ T+GHD
Sbjct: 450 C*PGWRGHACLSWCLWGSAALSATFWCGTEGHD 548
Score = 39.1 bits (79), Expect(2) = 7e-041
Identities = 20/54 (37%), Positives = 28/54 (51%)
Frame = -1 / -3
Query: 577 HDPLSSCYRYLGELWSPINTRVSSYTHANLTSTSIMVMLALLLLFRFFISPLSS 416
HD S R L E SPI+TR+ + HA+L + +++ L LL F P S
Sbjct: 546 HDLRSRTKR*LRERRSPISTRIGMHAHASLVNIVVLLTLLLLFRFLIIPLPFFS 385
Score = 29.9 bits (59), Expect(2) = 2e-048
Identities = 16/29 (55%), Positives = 16/29 (55%)
Frame = -2 / -1
Query: 579 IMTLCPPAIGIWVSCGAP*TPG*AVTPMP 493
IMT P G S GAP* PG*A P P
Sbjct: 548 IMTFGPAPKGS*ESGGAP*APG*ACMPTP 462
>gb|AL503599.1|AL503599 AL503599 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW02L23T 5', mRNA sequence
Length = 675
Score = 221 bits (478), Expect(2) = 1e-064
Identities = 92/128 (71%), Positives = 101/128 (78%)
Frame = +2 / +1
Query: 116 IPPNVTIYXXXXXXXXXXXXXXXSLTAVFSQFGKILDVLAFKTLKHKGQAWVVFEDVASA 295
+ PNVTIY SL AVFSQFGKI++VLAFKTLKHKGQAWVVFEDVASA
Sbjct: 112 VQPNVTIYINNLNEKIKLDALKKSLNAVFSQFGKIIEVLAFKTLKHKGQAWVVFEDVASA 291
Query: 296 TEALKRMQGFPFYDKTMRIQYAKTKSDIIAKADGTFVPRERRKRTDEKSEKKQKREHHHD 475
TEALKRMQ FPFYDK M+IQYAKTKSD++AKADGTFVPRE+RKR DEK EKK KRE HHD
Sbjct: 292 TEALKRMQDFPFYDKPMKIQYAKTKSDVVAKADGTFVPREKRKRNDEKPEKKHKREQHHD 471
Query: 476 AGASQIGM 499
+ +GM
Sbjct: 472 VNQAGVGM 495
Score = 182 bits (392), Expect(3) = 1e-051
Identities = 81/136 (59%), Positives = 98/136 (72%)
Frame = -1 / -1
Query: 499 HANLTSTSIMVMLALLLLFRFFISPLSSFSRYKGAISLCYNI*FGLGILNSHSFVIEGKA 320
HA+ + +I+V+L L+LLFRF I PL FSRY+GAIS CYNI* GLGILN H FVIEG+
Sbjct: 495 HAHASLVNIVVLLTLMLLFRFLIIPLPFFSRYEGAISFCYNI*LGLGILNFHGFVIEGEI 316
Query: 319 LHPLQSLSS*CDIFEDNPSLTFMLQSLESKHIKNFPXXXXXXXXXXXKFFELDLLVEVVD 140
LHPLQSL *C+IFE +P L FMLQSL+SKH+ +FP K E DLLVEV+D
Sbjct: 315 LHPLQSLGC*CNIFEHDPCLPFMLQSLKSKHLNDFPKLGENRIEGLLKCVEFDLLVEVID 136
Query: 139 IDGDVRRDAIRLSFAS 92
+DG V DA+RL A+
Sbjct: 135 VDGHVWLDAVRLPAAA 88
Score = 153 bits (329), Expect(2) = 6e-043
Identities = 75/120 (62%), Positives = 83/120 (69%)
Frame = +1 / +3
Query: 118 PAERHHLYQQPQREDQARRT*EVPNSRFFPVWENS*CACFQDFEAXXXXXXXXXXXXISY 297
PA+R HL+Q PQREDQ RRT*EVP F PVWEN * ACF DFEA IS
Sbjct: 114 PAKRDHLHQ*PQREDQTRRT*EVPQCGFLPVWENH*GACF*DFEA*RASMGRVRRCCISN 293
Query: 298 *GSEEDAGLSLL*QNYENSICQDQIRYYSKG*WHLCTSRKKKED**KI*KEAEARASP*C 477
G EEDA LL*Q +ENSICQDQ+R SK *WHL TSRKK+E+**+ *KEA A A+P C
Sbjct: 294 RGFEEDARFPLL*QTHENSICQDQVRCCSKS*WHLRTSRKKEEE**ET*KEA*A*AAPRC 473
Score = 150 bits (322), Expect = 1e-035
Identities = 76/135 (56%), Positives = 86/135 (63%)
Frame = -3 / -3
Query: 521 HQGKQLHPCQFD*HQHHGDARXXXXXXXXXXXXXXFLEVQRCHQPLL*YLIWSWHIEFS* 342
H+ + H C H G A FLEV+RCHQ LL +L WSWHIEFS
Sbjct: 517 HKHQDRHACPRQPG*HRGAAHAYASFQVSHHSSSFFLEVRRCHQLLLQHLTWSWHIEFSW 338
Query: 341 FCHRRESPASSSEPQ*LMRHLRRQPKLDLYASKS*KQAHQEFSQTGKKRLLGTS*VLRA* 162
CHRR + ASSS+P+ LM+HLR +P L LYASKS*KQA Q FSQTG+K GTS*V R *
Sbjct: 337 VCHRRGNLASSSKPRLLMQHLRTRPMLALYASKS*KQAPQ*FSQTGRKPH*GTS*VRRV* 158
Query: 161 SSR*GC*YRW*RSAG 117
SSR*G * RW R AG
Sbjct: 157 SSR*GY*CRWSRLAG 113
Score = 149 bits (319), Expect(3) = 4e-044
Identities = 70/97 (72%), Positives = 73/97 (75%)
Frame = -2 / -2
Query: 411 RGTKVPSAFAIISDLVLAY*ILIVLS*KGKPCILFRASVADATSSKTTQA*PLCFKVLKA 232
RGTKVPSAFA SDLVLAY*I + LS*KGK CILF+ASVADATSS TT A PLCFKVLKA
Sbjct: 407 RGTKVPSAFATTSDLVLAY*IFMGLS*KGKSCILFKASVADATSSNTTHACPLCFKVLKA 228
Query: 231 STSRIFPNWEKTAVRDXXXXXXXXXXXXXXX*MVTFG 121
STS IFPNWEKTA+RD *MVTFG
Sbjct: 227 STSMIFPNWEKTALRDFLSASSLIFSLRLLM*MVTFG 117
Score = 142 bits (305), Expect = 3e-033
Identities = 71/124 (57%), Positives = 80/124 (64%)
Frame = +3 / +2
Query: 105 RRMAXXXXXXXXXXXXXXXXXXKNLRSP*QPFFPSLGKFLMCLLSRL*SIKVKLGLSSKM 284
RR A +LRSP F PSLGK L CLL RL*SIK K G SKM
Sbjct: 101 RRTASSQT*PSTSITSTRRSNSTHLRSPSMRFSPSLGKSLRCLLLRL*SIKGKHGSCSKM 280
Query: 285 SHQLLRL*RGCRAFPSMTKL*EFNMPRPNQIL*QRLMAPLYLEKEERGLMKNLKRSRSAS 464
HQ RL*RGC+ PSMT *+FNMPRP+Q+L*Q+LMAP YLEK+ RG+M+NLKRS S S
Sbjct: 281 LHQQPRL*RGCKISPSMTNP*KFNMPRPSQML*QKLMAPSYLEKKGRGMMRNLKRSISVS 460
Query: 465 ITMM 476
T M
Sbjct: 461 STTM 472
Score = 46.0 bits (94), Expect(2) = 1e-064
Identities = 20/44 (45%), Positives = 28/44 (63%)
Frame = +2 / +3
Query: 521 VYGAPQLTQIPIAGGQRVMMPEIIVPNNILFVQNLPHETTPMML 652
++G+ L+ G +R +IIVPNNILFVQ LPHE P ++
Sbjct: 513 LWGSAALSATFWCGTRRS*CQKIIVPNNILFVQELPHERDPFLM 644
Score = 41.4 bits (84), Expect(2) = 6e-043
Identities = 14/25 (56%), Positives = 15/25 (60%)
Frame = +1 / +3
Query: 481 C*SNWHGCNCLPWCLWGSTAHPDTY 555
C* W G CL WCLWGS A T+
Sbjct: 471 C*PGWRGHACLSWCLWGSAALSATF 545
Score = 35.0 bits (70), Expect(3) = 4e-044
Identities = 17/46 (36%), Positives = 25/46 (54%)
Frame = -1 / -1
Query: 553 RYLGELWSPINTRVSSYTHANLTSTSIMVMLALLLLFRFFISPLSS 416
R L E SPI+TR+ + HA+L + +++ L LL F P S
Sbjct: 543 R*LRERRSPISTRIGMHAHASLVNIVVLLTLMLLFRFLIIPLPFFS 406
Score = 33.1 bits (66), Expect(3) = 4e-044
Identities = 9/16 (56%), Positives = 15/16 (93%)
Frame = -1 / -2
Query: 631 VWQVLDKQDVVWYDNL 584
VW+ LD++++VWY+NL
Sbjct: 623 VWEFLDEENIVWYNNL 576
Score = 33.1 bits (66), Expect(3) = 1e-051
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = -2 / -3
Query: 642 GVVSCGKFWTNKMLFGTII 586
G SCG WT ++LFGTII
Sbjct: 634 GSRSCGSSWTKRILFGTII 578
Score = 27.2 bits (53), Expect(3) = 1e-051
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = -3 / -3
Query: 539 AVEPHKHQGKQLHPCQFD*HQ 477
A EPHKHQ + P Q *H+
Sbjct: 529 AAEPHKHQDRHACPRQPG*HR 467
>gb|BU967835.1|BU967835 HB05I11r BC Hordeum vulgare subsp. vulgare cDNA clone HB05I11
5-PRIME, mRNA sequence
Length = 468
Score = 171 bits (367), Expect(2) = 5e-064
Identities = 67/84 (79%), Positives = 75/84 (89%)
Frame = +2 / +1
Query: 248 KHKGQAWVVFEDVASATEALKRMQGFPFYDKTMRIQYAKTKSDIIAKADGTFVPRERRKR 427
+H+GQAWVVFEDVASATEALKRMQ FPFYDK M+IQYAKTKSD++AKADGTFVPRE+RKR
Sbjct: 1 RHEGQAWVVFEDVASATEALKRMQDFPFYDKPMKIQYAKTKSDVVAKADGTFVPREKRKR 180
Query: 428 TDEKSEKKQKREHHHDAGASQIGM 499
DEK EKKQKRE HHD + +GM
Sbjct: 181 NDEKPEKKQKREQHHDVNQAGVGM 252
Score = 159 bits (341), Expect = 3e-038
Identities = 63/102 (61%), Positives = 76/102 (74%)
Frame = +2 / +1
Query: 416 RRKRTDEKSEKKQKREHHHDAGASQIGMGVTAYPGVYGAPQLTQIPIAGGQRVMMPEIIV 595
R KR + ++K++ +Q G+G+ AYPG YGAP L+Q+P G +VMMPEIIV
Sbjct: 163 REKRKRNDEKPEKKQKREQHHDVNQAGVGMHAYPGAYGAPPLSQLPFGAGPKVMMPEIIV 342
Query: 596 PNNILFVQNLPHETTPMMLQMLFCQYPGFKEVRMIEAKPGIA 721
PNNILFVQ LPH+ T +MLQM FCQYPGFKEVRMIEAKPGIA
Sbjct: 343 PNNILFVQELPHDATALMLQMFFCQYPGFKEVRMIEAKPGIA 468
Score = 126 bits (270), Expect(2) = 1e-057
Identities = 54/80 (67%), Positives = 63/80 (78%)
Frame = -1 / -1
Query: 499 HANLTSTSIMVMLALLLLFRFFISPLSSFSRYKGAISLCYNI*FGLGILNSHSFVIEGKA 320
HA+ + +I+V+L LLLLFRF I PL FSRY+GAIS CYNI* GLGILN H FVIEG+
Sbjct: 252 HAHASLVNIVVLLTLLLLFRFLIIPLPFFSRYEGAISFCYNI*LGLGILNFHGFVIEGEI 73
Query: 319 LHPLQSLSS*CDIFEDNPSL 260
LHPLQSL *C+IFE +P L
Sbjct: 72 LHPLQSLGC*CNIFEHDPCL 13
Score = 119 bits (254), Expect(2) = 5e-048
Identities = 49/102 (48%), Positives = 66/102 (64%)
Frame = -1 / -1
Query: 721 SNPRLRLDHPNLLEARVLAEEHLEHHGRRLVWQVLDKQDVVWYDNLWHHDPLSSCYRYLG 542
SN RL DHP+ LE VLAEEHL+H R+VW+ LD++++VWY+NLWHHD S R L
Sbjct: 468 SNTRLCFDHPHFLEPWVLAEEHLQHQSGRVVWEFLDEENIVWYNNLWHHDLRSRTKR*LR 289
Query: 541 ELWSPINTRVSSYTHANLTSTSIMVMLALLLLFRFFISPLSS 416
E SPI+TR+ + HA+L + +++ L LL F P S
Sbjct: 288 ERRSPISTRIGMHAHASLVNIVVLLTLLLLFRFLIIPLPFFS 163
Score = 117 bits (250), Expect(2) = 1e-057
Identities = 52/76 (68%), Positives = 55/76 (72%)
Frame = -2 / -2
Query: 720 AIPGFASIIRTSLKPGYWQKSIWSIMGVVSCGKFWTNKMLFGTIISGIMTLCPPAIGIWV 541
AIPGFASIIRTSL PGYWQK+I SI V SCG WT ++LFGTIISGIMT P G
Sbjct: 467 AIPGFASIIRTSLNPGYWQKNICSIKAVASCGSSWTKRILFGTIISGIMTFGPAPKGS*E 288
Query: 540 SCGAP*TPG*AVTPMP 493
S GAP* PG*A P P
Sbjct: 287 SGGAP*APG*ACMPTP 240
Score = 108 bits (231), Expect(2) = 8e-028
Identities = 49/72 (68%), Positives = 57/72 (79%)
Frame = +3 / +2
Query: 261 KLGLSSKMSHQLLRL*RGCRAFPSMTKL*EFNMPRPNQIL*QRLMAPLYLEKEERGLMKN 440
K G SKM HQ RL*RGC+ PSMT *+FNMPRP+Q+L*Q+LMAP YLEK+ RG+M+N
Sbjct: 14 KHGSCSKMLHQQPRL*RGCKISPSMTNP*KFNMPRPSQML*QKLMAPSYLEKKGRGMMRN 193
Query: 441 LKRSRSASITMM 476
LKRSRS S T M
Sbjct: 194 LKRSRSVSSTTM 229
Score = 94.5 bits (200), Expect(2) = 5e-064
Identities = 38/80 (47%), Positives = 46/80 (57%)
Frame = +1 / +3
Query: 481 C*SNWHGCNCLPWCLWGSTAHPDTYSRRTKGHDARDYRTKQHLVCPKLATRXXXXXXXXX 660
C* W G CL WCLWGS A T+ T+GHDARDY TKQ+ + P+ TR
Sbjct: 228 C*PGWRGHACLSWCLWGSAALSATFWCGTEGHDARDYCTKQYSLRPRTPTRRDRFDAADV 407
Query: 661 XXXVPWLQGGSDDRGEAWDC 720
VP +QG +DDR +AW C
Sbjct: 408 LLPVPRVQGSADDRSKAWYC 467
Score = 92.7 bits (196), Expect(2) = 5e-048
Identities = 43/54 (79%), Positives = 45/54 (83%)
Frame = -2 / -2
Query: 411 RGTKVPSAFAIISDLVLAY*ILIVLS*KGKPCILFRASVADATSSKTTQA*PLC 250
RGTKVPSAFA SDLVLAY*I + LS*KGK CILF+ASVADATSS TT A P C
Sbjct: 164 RGTKVPSAFATTSDLVLAY*IFMGLS*KGKSCILFKASVADATSSNTTHACPSC 3
Score = 35.4 bits (71), Expect(2) = 8e-028
Identities = 18/32 (56%), Positives = 19/32 (59%)
Frame = +3 / +2
Query: 525 MGLHSSPRYL*QEDKGS*CQRLSYQTTSCLSK 620
MGL S YL D+ S*CQRL YQT SK
Sbjct: 272 MGLRRSLSYLLVRDRRS*CQRLLYQTIFSSSK 367
>gb|BF619204.2|BF619204 HVSMEc0015B21f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0015B21f, mRNA sequence
Length = 566
Score = 194 bits (419), Expect(4) = 2e-062
Identities = 82/113 (72%), Positives = 90/113 (79%)
Frame = +2 / +3
Query: 116 IPPNVTIYXXXXXXXXXXXXXXXSLTAVFSQFGKILDVLAFKTLKHKGQAWVVFEDVASA 295
+ PNVTIY SL AVFSQFGKI++VLAFKTLKHKGQAWVVFEDVASA
Sbjct: 84 VQPNVTIYINNLNEKIKLDALKKSLNAVFSQFGKIIEVLAFKTLKHKGQAWVVFEDVASA 263
Query: 296 TEALKRMQGFPFYDKTMRIQYAKTKSDIIAKADGTFVPRERRKRTDEKSEKKQ 454
TEALKRMQ FPFYDK M+IQYAKTKSD++AKADG FVPRE+RKR DEK EK +
Sbjct: 264 TEALKRMQDFPFYDKPMKIQYAKTKSDVVAKADGPFVPREKRKRNDEKPEKNR 422
Score = 166 bits (356), Expect(3) = 9e-048
Identities = 74/120 (61%), Positives = 86/120 (71%)
Frame = -1 / -1
Query: 451 LLFRFFISPLSSFSRYKGAISLCYNI*FGLGILNSHSFVIEGKALHPLQSLSS*CDIFED 272
+LFRF I PL FSRY+GAIS CYNI* GLGILN H FVIEG+ LHPLQSL *C+IFE
Sbjct: 419 VLFRFLIIPLPFFSRYEGAISFCYNI*LGLGILNFHGFVIEGEILHPLQSLGC*CNIFEH 240
Query: 271 NPSLTFMLQSLESKHIKNFPXXXXXXXXXXXKFFELDLLVEVVDIDGDVRRDAIRLSFAS 92
+P L FMLQSL+SKH+ +FP K E DLLVEV+D+DG V DA+RL A+
Sbjct: 239 DPCLPFMLQSLKSKHLNDFPKLGENRIEGLLKCVEFDLLVEVIDVDGHVWLDAVRLPAAA 60
Score = 145 bits (312), Expect(3) = 6e-039
Identities = 69/97 (71%), Positives = 72/97 (74%)
Frame = -2 / -2
Query: 411 RGTKVPSAFAIISDLVLAY*ILIVLS*KGKPCILFRASVADATSSKTTQA*PLCFKVLKA 232
RGTK PSAFA SDLVLAY*I + LS*KGK CILF+ASVADATSS TT A PLCFKVLKA
Sbjct: 379 RGTKGPSAFATTSDLVLAY*IFMGLS*KGKSCILFKASVADATSSNTTHACPLCFKVLKA 200
Query: 231 STSRIFPNWEKTAVRDXXXXXXXXXXXXXXX*MVTFG 121
STS IFPNWEKTA+RD *MVTFG
Sbjct: 199 STSMIFPNWEKTALRDFLSASSLIFSLRLLM*MVTFG 89
Score = 141 bits (302), Expect(2) = 3e-035
Identities = 69/111 (62%), Positives = 76/111 (68%)
Frame = +1 / +2
Query: 118 PAERHHLYQQPQREDQARRT*EVPNSRFFPVWENS*CACFQDFEAXXXXXXXXXXXXISY 297
PA+R HL+Q PQREDQ RRT*EVP F PVWEN * ACF DFEA IS
Sbjct: 86 PAKRDHLHQ*PQREDQTRRT*EVPQCGFLPVWENH*GACF*DFEA*RASMGRVRRCCISN 265
Query: 298 *GSEEDAGLSLL*QNYENSICQDQIRYYSKG*WHLCTSRKKKED**KI*KE 450
G EEDA LL*Q +ENSICQDQ+R SK *W L TSRKK+E+**+ *KE
Sbjct: 266 RGFEEDARFPLL*QTHENSICQDQVRCCSKS*WPLRTSRKKEEE**ET*KE 418
Score = 137 bits (294), Expect = 1e-031
Identities = 68/99 (68%), Positives = 76/99 (76%)
Frame = -3 / -3
Query: 413 LEVQRCHQPLL*YLIWSWHIEFS*FCHRRESPASSSEPQ*LMRHLRRQPKLDLYASKS*K 234
LEV+R HQ LL +L WSWHIEFS CHRR + ASSS+P+ LM+HLR +P L LYASKS*K
Sbjct: 381 LEVRRGHQLLLQHLTWSWHIEFSWVCHRRGNLASSSKPRLLMQHLRTRPMLALYASKS*K 202
Query: 233 QAHQEFSQTGKKRLLGTS*VLRA*SSR*GC*YRW*RSAG 117
QA Q FSQTG+K GTS*V R *SSR*G * RW R AG
Sbjct: 201 QAPQ*FSQTGRKPH*GTS*VRRV*SSR*GY*CRWSRLAG 85
Score = 136 bits (291), Expect(2) = 2e-033
Identities = 66/118 (55%), Positives = 77/118 (65%)
Frame = +3 / +1
Query: 105 RRMAXXXXXXXXXXXXXXXXXXKNLRSP*QPFFPSLGKFLMCLLSRL*SIKVKLGLSSKM 284
RR A +LRSP F PSLGK L CLL RL*SIK K G SKM
Sbjct: 73 RRTASSQT*PSTSITSTRRSNSTHLRSPSMRFSPSLGKSLRCLLLRL*SIKGKHGSCSKM 252
Query: 285 SHQLLRL*RGCRAFPSMTKL*EFNMPRPNQIL*QRLMAPLYLEKEERGLMKNLKRSRS 458
HQ RL*RGC+ PSMT *+FNMPRP+Q+L*Q+LMAP YLEK+ RG+M+NLKR+ +
Sbjct: 253 LHQQPRL*RGCKISPSMTNP*KFNMPRPSQML*QKLMAPSYLEKKGRGMMRNLKRTEA 426
Score = 37.7 bits (76), Expect(4) = 2e-062
Identities = 12/20 (60%), Positives = 12/20 (60%)
Frame = +1 / +1
Query: 481 C*SNWHGCNCLPWCLWGSTA 540
C* W G CL WCLWG A
Sbjct: 442 C*PGWRGHACLSWCLWGFAA 501
Score = 34.1 bits (68), Expect(4) = 2e-062
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = +2 / +3
Query: 527 GAPQLTQIPIAGGQRVMMPEIIVPN 601
G + +P G +VMMPEIIVPN
Sbjct: 489 GLRRSLSLPFGAGPKVMMPEIIVPN 563
Score = 33.1 bits (66), Expect(3) = 9e-048
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = -1 / -1
Query: 601 VWYDNLWHHD 572
VWY+NLWHHD
Sbjct: 563 VWYNNLWHHD 534
Score = 30.4 bits (60), Expect(4) = 2e-062
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = +2 / +2
Query: 446 KKQKREHHHDAGASQIGM 499
K+QKRE HHD + +GM
Sbjct: 413 KEQKREQHHDVNQAGVGM 466
Score = 30.4 bits (60), Expect(3) = 9e-048
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = -1 / -2
Query: 547 LGELWSPINTRVSSYTHANLTSTSIMVMLALLLLFRFFI 431
L E SPI+TR+ + HA+L + +++ L F F+
Sbjct: 508 LRERRSPISTRIGMHAHASLVNIVVLLTLLFFSGFSSFL 392
Score = 28.6 bits (56), Expect(3) = 6e-039
Identities = 12/18 (66%), Positives = 13/18 (72%)
Frame = -2 / -2
Query: 603 LFGTIISGIMTLCPPAIG 550
+FGTIISGIMT P G
Sbjct: 565 MFGTIISGIMTFGPAPKG 512
Score = 28.1 bits (55), Expect(2) = 3e-035
Identities = 13/25 (52%), Positives = 15/25 (60%)
Frame = +3 / +1
Query: 528 GLHSSPRYL*QEDKGS*CQRLSYQT 602
G + YL D+ S*CQRL YQT
Sbjct: 490 GFAALSAYLLGRDRKS*CQRLLYQT 564
Score = 27.2 bits (53), Expect(2) = 2e-033
Identities = 13/34 (38%), Positives = 16/34 (47%)
Frame = +1 / +2
Query: 499 GCNCLPWCLWGSTAHPDTYSRRTKGHDARDYRTK 600
G + P S T+ T+ HDARDY TK
Sbjct: 461 GMHAYPGAYGASPLSQLTFWGGTESHDARDYCTK 562
Score = 25.4 bits (49), Expect(3) = 6e-039
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = -3 / -1
Query: 539 AVEPHKHQGKQLHPCQFD*HQ 477
A +PHKHQ + P Q *H+
Sbjct: 500 AAKPHKHQDRHACPRQPG*HR 438
>gb|BM816161.1|BM816161 HC108D04_SK.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC108D04_SK.ab1 similar to (AC067971) Strong similarity
to a small nuclear ribonucleoprotein U2B'' - potato from
Solanum tuberosum gb|M72892. It contains an RNA
recognition motif PF|00076. ESTs gb|AA041158 and
gb|AI992475 come from this gene. [Arabidopsis thaliana],
mRNA sequence
Length = 929
Score = 139 bits (298), Expect(3) = 4e-059
Identities = 55/115 (47%), Positives = 79/115 (68%)
Frame = +2 / +1
Query: 116 IPPNVTIYXXXXXXXXXXXXXXXSLTAVFSQFGKILDVLAFKTLKHKGQAWVVFEDVASA 295
+PPN T+Y SL A+ SQ+G+I+DV+A KT K +GQAWV F ++ +A
Sbjct: 100 VPPNQTVYFKNLNEKVKKEELKRSLYALCSQYGRIVDVVALKTHKLRGQAWVAFSEITAA 279
Query: 296 TEALKRMQGFPFYDKTMRIQYAKTKSDIIAKADGTFVPRERRKRTDEKSEKKQKR 460
T A + +Q F FY K MR+QYAKTKSD AKADG++ P+E+RK+ +EK+ +K++R
Sbjct: 280 TNAFRGLQDFDFYGKKMRVQYAKTKSDCFAKADGSYAPKEKRKKQEEKAAEKKRR 444
Score = 99.6 bits (211), Expect(3) = 4e-059
Identities = 40/56 (71%), Positives = 48/56 (85%)
Frame = +2 / +1
Query: 596 PNNILFVQNLPHETTPMMLQMLFCQYPGFKEVRMIEAKPGIAFVEYGDETQATAAM 763
PNNILF+QNLP +TT +MLQ+LF QYPGF+EVRMIEA+PGIAF EY DE Q+ A+
Sbjct: 553 PNNILFLQNLPDQTTSVMLQILFQQYPGFREVRMIEAQPGIAFGEYEDENQSMVAL 720
Score = 85.4 bits (180), Expect(2) = 7e-026
Identities = 38/69 (55%), Positives = 44/69 (63%)
Frame = -2 / -1
Query: 801 WFSFVILKL*RLFMAAVAWVSSPYSTKAIPGFASIIRTSLKPGYWQKSIWSIMGVVSCGK 622
W + L+ F A + W SS YS KA+PG+ASIIRTS KPGY IWSI VV GK
Sbjct: 758 WVLWADLETLEGFNATMDWFSSSYSPKAMPGWASIIRTSRKPGYCWNRIWSITLVVWSGK 579
Query: 621 FWTNKMLFG 595
FW +MLFG
Sbjct: 578 FWRKRMLFG 552
Score = 55.6 bits (115), Expect(2) = 7e-026
Identities = 39/93 (41%), Positives = 41/93 (44%)
Frame = -2 / -1
Query: 396 PSAFAIISDLVLAY*ILIVLS*KGKPCILFRASVADATSSKTTQA*PLCFKVLKASTSRI 217
PSA A SD V AY I L *K K C +A VA S TQA PL V KA+TS I
Sbjct: 380 PSALAKQSDFVFAYCTRIFLP*KSKSCRPRKALVAAVISLNATQACPLSL*VFKATTSTI 201
Query: 216 FPNWEKTAVRDXXXXXXXXXXXXXXX*MVTFGG 118
P E A D * V FGG
Sbjct: 200 LPY*EHNA*SDLFSSSFLTFSLRFLK*TVWFGG 102
Score = 34.1 bits (68), Expect(3) = 4e-059
Identities = 15/23 (65%), Positives = 17/23 (73%)
Frame = +2 / +3
Query: 770 LQSFKITKENQMVITYAKK**AP 838
LQ FKI+ EN M I+YAKK* P
Sbjct: 726 LQGFKISPENPMAISYAKK*TIP 794
>gb|BU979480.1|BU979480 HA16E20r HA Hordeum vulgare subsp. vulgare cDNA clone HA16E20
5-PRIME, mRNA sequence
Length = 506
Score = 224 bits (483), Expect = 8e-058
Identities = 93/128 (72%), Positives = 102/128 (79%)
Frame = +2 / +1
Query: 116 IPPNVTIYXXXXXXXXXXXXXXXSLTAVFSQFGKILDVLAFKTLKHKGQAWVVFEDVASA 295
+ PNVTIY SL AVFSQFGKI++VLAFKTLKHKGQAWVVFEDVASA
Sbjct: 97 VQPNVTIYINNLNEKIKLDALKKSLNAVFSQFGKIIEVLAFKTLKHKGQAWVVFEDVASA 276
Query: 296 TEALKRMQGFPFYDKTMRIQYAKTKSDIIAKADGTFVPRERRKRTDEKSEKKQKREHHHD 475
TEALKRMQ FPFYDK M+IQYAKTKSD++AKADGTFVPRE+RKR DEK EKKQKRE HHD
Sbjct: 277 TEALKRMQDFPFYDKPMKIQYAKTKSDVVAKADGTFVPREKRKRNDEKPEKKQKREQHHD 456
Query: 476 AGASQIGM 499
+ +GM
Sbjct: 457 VNQAGVGM 480
Score = 183 bits (394), Expect = 2e-045
Identities = 82/136 (60%), Positives = 98/136 (72%)
Frame = -1 / -3
Query: 499 HANLTSTSIMVMLALLLLFRFFISPLSSFSRYKGAISLCYNI*FGLGILNSHSFVIEGKA 320
HA+ + +I+V+L LLLLFRF I PL FSRY+GAIS CYNI* GLGILN H FVIEG+
Sbjct: 480 HAHASLVNIVVLLTLLLLFRFLIIPLPFFSRYEGAISFCYNI*LGLGILNFHGFVIEGEI 301
Query: 319 LHPLQSLSS*CDIFEDNPSLTFMLQSLESKHIKNFPXXXXXXXXXXXKFFELDLLVEVVD 140
LHPLQSL *C+IFE +P L FMLQSL+SKH+ +FP K E DLLVEV+D
Sbjct: 300 LHPLQSLGC*CNIFEHDPCLPFMLQSLKSKHLNDFPKLGENRIEGLLKCVEFDLLVEVID 121
Query: 139 IDGDVRRDAIRLSFAS 92
+DG V DA+RL A+
Sbjct: 120 VDGHVWLDAVRLPAAA 73
Score = 157 bits (338), Expect(2) = 8e-043
Identities = 76/120 (63%), Positives = 84/120 (70%)
Frame = +1 / +3
Query: 118 PAERHHLYQQPQREDQARRT*EVPNSRFFPVWENS*CACFQDFEAXXXXXXXXXXXXISY 297
PA+R HL+Q PQREDQ RRT*EVP F PVWEN * ACF DFEA IS
Sbjct: 99 PAKRDHLHQ*PQREDQTRRT*EVPQCGFLPVWENH*GACF*DFEA*RASMGRVRRCCISN 278
Query: 298 *GSEEDAGLSLL*QNYENSICQDQIRYYSKG*WHLCTSRKKKED**KI*KEAEARASP*C 477
G EEDA LL*Q +ENSICQDQ+R SK *WHL TSRKK+E+**+ *KEAEA A+P C
Sbjct: 279 RGFEEDARFPLL*QTHENSICQDQVRCCSKS*WHLRTSRKKEEE**ET*KEAEA*AAPRC 458
Score = 150 bits (323), Expect = 1e-035
Identities = 76/135 (56%), Positives = 86/135 (63%)
Frame = -3 / -2
Query: 521 HQGKQLHPCQFD*HQHHGDARXXXXXXXXXXXXXXFLEVQRCHQPLL*YLIWSWHIEFS* 342
H+ + H C H G A FLEV+RCHQ LL +L WSWHIEFS
Sbjct: 502 HKHQDRHACPRQPG*HRGAAHASASFQVSHHSSSFFLEVRRCHQLLLQHLTWSWHIEFSW 323
Query: 341 FCHRRESPASSSEPQ*LMRHLRRQPKLDLYASKS*KQAHQEFSQTGKKRLLGTS*VLRA* 162
CHRR + ASSS+P+ LM+HLR +P L LYASKS*KQA Q FSQTG+K GTS*V R *
Sbjct: 322 VCHRRGNLASSSKPRLLMQHLRTRPMLALYASKS*KQAPQ*FSQTGRKPH*GTS*VRRV* 143
Query: 161 SSR*GC*YRW*RSAG 117
SSR*G * RW R AG
Sbjct: 142 SSR*GY*CRWSRLAG 98
Score = 149 bits (319), Expect(2) = 1e-038
Identities = 70/97 (72%), Positives = 73/97 (75%)
Frame = -2 / -1
Query: 411 RGTKVPSAFAIISDLVLAY*ILIVLS*KGKPCILFRASVADATSSKTTQA*PLCFKVLKA 232
RGTKVPSAFA SDLVLAY*I + LS*KGK CILF+ASVADATSS TT A PLCFKVLKA
Sbjct: 392 RGTKVPSAFATTSDLVLAY*IFMGLS*KGKSCILFKASVADATSSNTTHACPLCFKVLKA 213
Query: 231 STSRIFPNWEKTAVRDXXXXXXXXXXXXXXX*MVTFG 121
STS IFPNWEKTA+RD *MVTFG
Sbjct: 212 STSMIFPNWEKTALRDFLSASSLIFSLRLLM*MVTFG 102
Score = 146 bits (313), Expect = 2e-034
Identities = 72/124 (58%), Positives = 81/124 (65%)
Frame = +3 / +2
Query: 105 RRMAXXXXXXXXXXXXXXXXXXKNLRSP*QPFFPSLGKFLMCLLSRL*SIKVKLGLSSKM 284
RR A +LRSP F PSLGK L CLL RL*SIK K G SKM
Sbjct: 86 RRTASSQT*PSTSITSTRRSNSTHLRSPSMRFSPSLGKSLRCLLLRL*SIKGKHGSCSKM 265
Query: 285 SHQLLRL*RGCRAFPSMTKL*EFNMPRPNQIL*QRLMAPLYLEKEERGLMKNLKRSRSAS 464
HQ RL*RGC+ PSMT *+FNMPRP+Q+L*Q+LMAP YLEK+ RG+M+NLKRSRS S
Sbjct: 266 LHQQPRL*RGCKISPSMTNP*KFNMPRPSQML*QKLMAPSYLEKKGRGMMRNLKRSRSVS 445
Query: 465 ITMM 476
T M
Sbjct: 446 STTM 457
Score = 36.8 bits (74), Expect(2) = 8e-043
Identities = 11/17 (64%), Positives = 11/17 (64%)
Frame = +1 / +3
Query: 481 C*SNWHGCNCLPWCLWG 531
C* W G CL WCLWG
Sbjct: 456 C*PGWRGHACLSWCLWG 506
Score = 31.8 bits (63), Expect(2) = 1e-038
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = -1 / -3
Query: 529 PINTRVSSYTHANLTSTSIMVMLALLLLFRFFISPLSS 416
PI+TR+ + HA+L + +++ L LL F P S
Sbjct: 504 PISTRIGMHAHASLVNIVVLLTLLLLFRFLIIPLPFFS 391
>gb|BM816160.1|BM816160 HC108D03_SK.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC108D03_SK.ab1 similar to (AC067971) Strong similarity
to a small nuclear ribonucleoprotein U2B'' - potato from
Solanum tuberosum gb|M72892. It contains an RNA
recognition motif PF|00076. ESTs gb|AA041158 and
gb|AI992475 come from this gene. [Arabidopsis thaliana],
mRNA sequence
Length = 881
Score = 139 bits (298), Expect(2) = 3e-057
Identities = 55/115 (47%), Positives = 79/115 (68%)
Frame = +2 / +3
Query: 116 IPPNVTIYXXXXXXXXXXXXXXXSLTAVFSQFGKILDVLAFKTLKHKGQAWVVFEDVASA 295
+PPN T+Y SL A+ SQ+G+I+DV+A KT K +GQAWV F ++ +A
Sbjct: 99 VPPNQTVYFKNLNEKVKKEELKRSLYALCSQYGRIVDVVALKTHKLRGQAWVAFSEITAA 278
Query: 296 TEALKRMQGFPFYDKTMRIQYAKTKSDIIAKADGTFVPRERRKRTDEKSEKKQKR 460
T A + +Q F FY K MR+QYAKTKSD AKADG++ P+E+RK+ +EK+ +K++R
Sbjct: 279 TNAFRGLQDFDFYGKKMRVQYAKTKSDCFAKADGSYAPKEKRKKQEEKAAEKKRR 443
Score = 106 bits (226), Expect(2) = 3e-057
Identities = 46/82 (56%), Positives = 57/82 (69%)
Frame = +2 / +3
Query: 596 PNNILFVQNLPHETTPMMLQMLFCQYPGFKEVRMIEAKPGIAFVEYGDETQATAAMNNLQ 775
PNNILF+QNLP +TT +MLQ+LF QYPGF+EVRMIEAKPGIAFVEY Q+ M + Q
Sbjct: 552 PNNILFLQNLPDQTTSVMLQILFQQYPGFREVRMIEAKPGIAFVEYXGRDQSMGCMESXQ 731
Query: 776 SFKITKENQMVITYAKK**APE 841
+I+ E + K +PE
Sbjct: 732 GXQISPETPWLYHTPKSERSPE 797
Score = 80.3 bits (169), Expect(2) = 2e-024
Identities = 36/60 (60%), Positives = 39/60 (65%)
Frame = -2 / -2
Query: 774 *RLFMAAVAWVSSPYSTKAIPGFASIIRTSLKPGYWQKSIWSIMGVVSCGKFWTNKMLFG 595
* L M + W YSTKA+PG ASIIRTS KPGY IWSI VV GKFW +MLFG
Sbjct: 730 *XLSMQPMDWSRPSYSTKAMPGLASIIRTSRKPGYCWNRIWSITLVVWSGKFWRKRMLFG 551
Score = 55.6 bits (115), Expect(2) = 2e-024
Identities = 39/93 (41%), Positives = 41/93 (44%)
Frame = -2 / -2
Query: 396 PSAFAIISDLVLAY*ILIVLS*KGKPCILFRASVADATSSKTTQA*PLCFKVLKASTSRI 217
PSA A SD V AY I L *K K C +A VA S TQA PL V KA+TS I
Sbjct: 379 PSALAKQSDFVFAYCTRIFLP*KSKSCRPRKALVAAVISLNATQACPLSL*VFKATTSTI 200
Query: 216 FPNWEKTAVRDXXXXXXXXXXXXXXX*MVTFGG 118
P E A D * V FGG
Sbjct: 199 LPY*EHNA*SDLFSSSFLTFSLRFLK*TVWFGG 101
>gb|BE601776.2|BE601776 HVSMEh0099I05f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0099I05f, mRNA sequence
Length = 502
Score = 221 bits (478), Expect = 4e-057
Identities = 92/127 (72%), Positives = 101/127 (79%)
Frame = +2 / +1
Query: 116 IPPNVTIYXXXXXXXXXXXXXXXSLTAVFSQFGKILDVLAFKTLKHKGQAWVVFEDVASA 295
+ PNVTIY SL AVFSQFGKI++VLAFKTLKHKGQAWVVFEDVASA
Sbjct: 85 VKPNVTIYINNLNEKIKLDALKKSLNAVFSQFGKIIEVLAFKTLKHKGQAWVVFEDVASA 264
Query: 296 TEALKRMQGFPFYDKTMRIQYAKTKSDIIAKADGTFVPRERRKRTDEKSEKKQKREHHHD 475
TEALKRMQ FPFYDK M+IQYAKTKSD++AKADGTFVPRE+RKR DEK EKKQKRE HHD
Sbjct: 265 TEALKRMQDFPFYDKPMKIQYAKTKSDVVAKADGTFVPREKRKRNDEKPEKKQKREQHHD 444
Query: 476 AGASQIG 496
+ +G
Sbjct: 445 VNQAGVG 465
Score = 176 bits (379), Expect = 2e-043
Identities = 80/134 (59%), Positives = 95/134 (70%)
Frame = -1 / -2
Query: 493 NLTSTSIMVMLALLLLFRFFISPLSSFSRYKGAISLCYNI*FGLGILNSHSFVIEGKALH 314
N + +I+V+L LLLLFRF I PL FSRY+GAIS CYNI* GLGILN H FVIEG+ LH
Sbjct: 462 NASLVNIVVLLTLLLLFRFLIIPLPFFSRYEGAISFCYNI*LGLGILNFHGFVIEGEILH 283
Query: 313 PLQSLSS*CDIFEDNPSLTFMLQSLESKHIKNFPXXXXXXXXXXXKFFELDLLVEVVDID 134
PLQSL *C+IFE +P L FMLQSL+SKH+ +FP K E DLLVEV+D+D
Sbjct: 282 PLQSLGC*CNIFEHDPCLPFMLQSLKSKHLNDFPKLGENRIEGLLKCVEFDLLVEVIDVD 103
Query: 133 GDVRRDAIRLSFAS 92
G V DA+ L A+
Sbjct: 102 GHVWLDAVCLPAAA 61
Score = 156 bits (335), Expect = 2e-037
Identities = 77/128 (60%), Positives = 85/128 (66%)
Frame = +1 / +3
Query: 121 AERHHLYQQPQREDQARRT*EVPNSRFFPVWENS*CACFQDFEAXXXXXXXXXXXXISY* 300
A+R HL+Q PQREDQ RRT*EVP F PVWEN * ACF DFEA IS
Sbjct: 90 AKRDHLHQ*PQREDQTRRT*EVPQCGFLPVWENH*GACF*DFEA*RASMGRVRRCCISNR 269
Query: 301 GSEEDAGLSLL*QNYENSICQDQIRYYSKG*WHLCTSRKKKED**KI*KEAEARASP*CW 480
G EEDA LL*Q +ENSICQDQ+R SK *WHL TSRKK+E+**+ *KEAEA A+P C
Sbjct: 270 GFEEDARFPLL*QTHENSICQDQVRCCSKS*WHLRTSRKKEEE**ET*KEAEA*AAPRC* 449
Query: 481 C*SNWHGC 504
W C
Sbjct: 450 PGWRWGSC 473
Score = 149 bits (319), Expect = 3e-035
Identities = 70/97 (72%), Positives = 73/97 (75%)
Frame = -2 / -3
Query: 411 RGTKVPSAFAIISDLVLAY*ILIVLS*KGKPCILFRASVADATSSKTTQA*PLCFKVLKA 232
RGTKVPSAFA SDLVLAY*I + LS*KGK CILF+ASVADATSS TT A PLCFKVLKA
Sbjct: 380 RGTKVPSAFATTSDLVLAY*IFMGLS*KGKSCILFKASVADATSSNTTHACPLCFKVLKA 201
Query: 231 STSRIFPNWEKTAVRDXXXXXXXXXXXXXXX*MVTFG 121
STS IFPNWEKTA+RD *MVTFG
Sbjct: 200 STSMIFPNWEKTALRDFLSASSLIFSLRLLM*MVTFG 90
Score = 145 bits (312), Expect = 3e-034
Identities = 69/101 (68%), Positives = 78/101 (77%)
Frame = +3 / +2
Query: 174 NLRSP*QPFFPSLGKFLMCLLSRL*SIKVKLGLSSKMSHQLLRL*RGCRAFPSMTKL*EF 353
+LRSP F PSLGK L CLL RL*SIK K G SKM HQ RL*RGC+ PSMT *+F
Sbjct: 143 HLRSPSMRFSPSLGKSLRCLLLRL*SIKGKHGSCSKMLHQQPRL*RGCKISPSMTNP*KF 322
Query: 354 NMPRPNQIL*QRLMAPLYLEKEERGLMKNLKRSRSASITMM 476
NMPRP+Q+L*Q+LMAP YLEK+ RG+M+NLKRSRS S T M
Sbjct: 323 NMPRPSQML*QKLMAPSYLEKKGRGMMRNLKRSRSVSSTTM 445
Score = 144 bits (309), Expect = 8e-034
Identities = 72/119 (60%), Positives = 80/119 (67%)
Frame = -3 / -1
Query: 476 HHGDARXXXXXXXXXXXXXXFLEVQRCHQPLL*YLIWSWHIEFS*FCHRRESPASSSEPQ 297
H G A FLEV+RCHQ LL +L WSWHIEFS CHRR + ASSS+P+
Sbjct: 445 HRGAAHASASFQVSHHSSSFFLEVRRCHQLLLQHLTWSWHIEFSWVCHRRGNLASSSKPR 266
Query: 296 *LMRHLRRQPKLDLYASKS*KQAHQEFSQTGKKRLLGTS*VLRA*SSR*GC*YRW*RSA 120
LM+HLR +P L LYASKS*KQA Q FSQTG+K GTS*V R *SSR*G * RW R A
Sbjct: 265 LLMQHLRTRPMLALYASKS*KQAPQ*FSQTGRKPH*GTS*VRRV*SSR*GY*CRWSRLA 89
>gb|CA001791.1|CA001791 HS05J13r HS Hordeum vulgare subsp. vulgare cDNA clone HS05J13
5-PRIME, mRNA sequence
Length = 416
Score = 218 bits (471), Expect = 4e-056
Identities = 91/120 (75%), Positives = 98/120 (81%)
Frame = +2 / +2
Query: 116 IPPNVTIYXXXXXXXXXXXXXXXSLTAVFSQFGKILDVLAFKTLKHKGQAWVVFEDVASA 295
+ PNVTIY SL AVFSQFGKI++VLAFKTLKHKGQAWVVFEDVASA
Sbjct: 50 VQPNVTIYINNLNEKIKLDALKKSLNAVFSQFGKIIEVLAFKTLKHKGQAWVVFEDVASA 229
Query: 296 TEALKRMQGFPFYDKTMRIQYAKTKSDIIAKADGTFVPRERRKRTDEKSEKKQKREHHHD 475
TEALKRMQ FPFYDK M+IQYAKTKSD++AKADGTFVPRE+RKR DEK EKKQKRE HHD
Sbjct: 230 TEALKRMQDFPFYDKPMKIQYAKTKSDVVAKADGTFVPREKRKRNDEKPEKKQKREQHHD 409
Score = 178 bits (383), Expect = 5e-044
Identities = 80/129 (62%), Positives = 94/129 (72%)
Frame = -1 / -2
Query: 478 SIMVMLALLLLFRFFISPLSSFSRYKGAISLCYNI*FGLGILNSHSFVIEGKALHPLQSL 299
+I+V+L LLLLFRF I PL FSRY+GAIS CYNI* GLGILN H FVIEG+ LHPLQSL
Sbjct: 412 NIVVLLTLLLLFRFLIIPLPFFSRYEGAISFCYNI*LGLGILNFHGFVIEGEILHPLQSL 233
Query: 298 SS*CDIFEDNPSLTFMLQSLESKHIKNFPXXXXXXXXXXXKFFELDLLVEVVDIDGDVRR 119
*C+IFE +P L FMLQSL+SKH+ +FP K E DLLVEV+D+DG V
Sbjct: 232 GC*CNIFEHDPCLPFMLQSLKSKHLNDFPKLGENRIEGLLKCVEFDLLVEVIDVDGHVWL 53
Query: 118 DAIRLSFAS 92
DA+RL A+
Sbjct: 52 DAVRLPAAA 26
Score = 157 bits (338), Expect = 8e-038
Identities = 76/120 (63%), Positives = 84/120 (70%)
Frame = +1 / +1
Query: 118 PAERHHLYQQPQREDQARRT*EVPNSRFFPVWENS*CACFQDFEAXXXXXXXXXXXXISY 297
PA+R HL+Q PQREDQ RRT*EVP F PVWEN * ACF DFEA IS
Sbjct: 52 PAKRDHLHQ*PQREDQTRRT*EVPQCGFLPVWENH*GACF*DFEA*RASMGRVRRCCISN 231
Query: 298 *GSEEDAGLSLL*QNYENSICQDQIRYYSKG*WHLCTSRKKKED**KI*KEAEARASP*C 477
G EEDA LL*Q +ENSICQDQ+R SK *WHL TSRKK+E+**+ *KEAEA A+P C
Sbjct: 232 RGFEEDARFPLL*QTHENSICQDQVRCCSKS*WHLRTSRKKEEE**ET*KEAEA*AAPRC 411
Score = 149 bits (319), Expect = 3e-035
Identities = 70/97 (72%), Positives = 73/97 (75%)
Frame = -2 / -3
Query: 411 RGTKVPSAFAIISDLVLAY*ILIVLS*KGKPCILFRASVADATSSKTTQA*PLCFKVLKA 232
RGTKVPSAFA SDLVLAY*I + LS*KGK CILF+ASVADATSS TT A PLCFKVLKA
Sbjct: 345 RGTKVPSAFATTSDLVLAY*IFMGLS*KGKSCILFKASVADATSSNTTHACPLCFKVLKA 166
Query: 231 STSRIFPNWEKTAVRDXXXXXXXXXXXXXXX*MVTFG 121
STS IFPNWEKTA+RD *MVTFG
Sbjct: 165 STSMIFPNWEKTALRDFLSASSLIFSLRLLM*MVTFG 55
Score = 147 bits (315), Expect = 1e-034
Identities = 73/120 (60%), Positives = 81/120 (67%)
Frame = -3 / -1
Query: 476 HHGDARXXXXXXXXXXXXXXFLEVQRCHQPLL*YLIWSWHIEFS*FCHRRESPASSSEPQ 297
H G A FLEV+RCHQ LL +L WSWHIEFS CHRR + ASSS+P+
Sbjct: 410 HRGAAHASASFQVSHHSSSFFLEVRRCHQLLLQHLTWSWHIEFSWVCHRRGNLASSSKPR 231
Query: 296 *LMRHLRRQPKLDLYASKS*KQAHQEFSQTGKKRLLGTS*VLRA*SSR*GC*YRW*RSAG 117
LM+HLR +P L LYASKS*KQA Q FSQTG+K GTS*V R *SSR*G * RW R AG
Sbjct: 230 LLMQHLRTRPMLALYASKS*KQAPQ*FSQTGRKPH*GTS*VRRV*SSR*GY*CRWSRLAG 51
Score = 146 bits (313), Expect = 2e-034
Identities = 72/124 (58%), Positives = 81/124 (65%)
Frame = +3 / +3
Query: 105 RRMAXXXXXXXXXXXXXXXXXXKNLRSP*QPFFPSLGKFLMCLLSRL*SIKVKLGLSSKM 284
RR A +LRSP F PSLGK L CLL RL*SIK K G SKM
Sbjct: 39 RRTASSQT*PSTSITSTRRSNSTHLRSPSMRFSPSLGKSLRCLLLRL*SIKGKHGSCSKM 218
Query: 285 SHQLLRL*RGCRAFPSMTKL*EFNMPRPNQIL*QRLMAPLYLEKEERGLMKNLKRSRSAS 464
HQ RL*RGC+ PSMT *+FNMPRP+Q+L*Q+LMAP YLEK+ RG+M+NLKRSRS S
Sbjct: 219 LHQQPRL*RGCKISPSMTNP*KFNMPRPSQML*QKLMAPSYLEKKGRGMMRNLKRSRSVS 398
Query: 465 ITMM 476
T M
Sbjct: 399 STTM 410
>gb|BF623183.2|BF623183 HVSMEa0015A20f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0015A20f, mRNA sequence
Length = 830
Score = 138 bits (297), Expect(3) = 7e-052
Identities = 54/78 (69%), Positives = 63/78 (80%)
Frame = +2 / +1
Query: 266 WVVFEDVASATEALKRMQGFPFYDKTMRIQYAKTKSDIIAKADGTFVPRERRKRTDEKSE 445
W +DVA ATEAL RM FPFYDK M+IQYAKTKS+++AKADGTFVPRE+RKR D+K E
Sbjct: 247 WFALQDVALATEALNRMHYFPFYDKPMKIQYAKTKSNVVAKADGTFVPREKRKRNDDKPE 426
Query: 446 KKQKREHHHDAGASQIGM 499
KKQKRE HHD + +GM
Sbjct: 427 KKQKREQHHDVNQAGVGM 480
Score = 129 bits (277), Expect = 2e-029
Identities = 53/98 (54%), Positives = 68/98 (69%)
Frame = +2 / +1
Query: 416 RRKRTDEKSEKKQKREHHHDAGASQIGMGVTAYPGVYGAPQLTQIPIAGGQRVMMPEIIV 595
R KR + ++K++ +Q G+G+ AYPG YGAP L+Q+P +VMMP+IIV
Sbjct: 391 REKRKRNDDKPEKKQKREQHHDVNQAGVGMHAYPGAYGAPPLSQLPFGAVPKVMMPDIIV 570
Query: 596 PNNILFVQNLPHETTPMMLQMLFCQYPGFKEVRMIEAK 709
PN+ILFVQ LPH+ T +MLQM F QYP F EVRMIEAK
Sbjct: 571 PNSILFVQELPHDATALMLQMFF*QYPWFNEVRMIEAK 684
Score = 116 bits (249), Expect(2) = 2e-046
Identities = 53/96 (55%), Positives = 66/96 (68%)
Frame = -1 / -3
Query: 499 HANLTSTSIMVMLALLLLFRFFISPLSSFSRYKGAISLCYNI*FGLGILNSHSFVIEGKA 320
HA+ + +I+V+L LLLLFRF I PL FSRY+GAIS CYNI* GLGILN H FVIEG+
Sbjct: 480 HAHASLVNIVVLLTLLLLFRFVIIPLPFFSRYEGAISFCYNI*LGLGILNFHGFVIEGEI 301
Query: 319 LHPLQSLSS*CDIFEDNPSLTFMLQSLESKHIKNFP 212
+HP+ SL *C+I P L L+ L++ FP
Sbjct: 300 VHPI*SLGC*CNIL*REPWLPLCLEVLKASPSMLFP 193
Score = 103 bits (220), Expect(3) = 3e-041
Identities = 42/99 (42%), Positives = 61/99 (61%)
Frame = -1 / -3
Query: 712 RLRLDHPNLLEARVLAEEHLEHHGRRLVWQVLDKQDVVWYDNLWHHDPLSSCYRYLGELW 533
+L DHP+ +E VL EEHL+H R+VW+ LD+++ VWY+N+WHHD R L E
Sbjct: 687 QLCFDHPHFVEPWVLLEEHLQHQSGRVVWEFLDEENTVWYNNIWHHDFRYRTKR*LRERR 508
Query: 532 SPINTRVSSYTHANLTSTSIMVMLALLLLFRFFISPLSS 416
SPI+TR+ + HA+L + +++ L LL F P S
Sbjct: 507 SPISTRIGMHAHASLVNIVVLLTLLLLFRFVIIPLPFFS 391
Score = 94.5 bits (200), Expect(2) = 2e-021
Identities = 45/70 (64%), Positives = 51/70 (72%)
Frame = +3 / +2
Query: 267 GLSSKMSHQLLRL*RGCRAFPSMTKL*EFNMPRPNQIL*QRLMAPLYLEKEERGLMKNLK 446
G KM H RL* GC PSMT *+FNMPRP+Q+L*Q+LMAP YLEK+ RG+M NLK
Sbjct: 248 GSRYKMLH*QPRL*IGCTISPSMTNP*KFNMPRPSQML*QKLMAPSYLEKKGRGMMTNLK 427
Query: 447 RSRSASITMM 476
RSRS S T M
Sbjct: 428 RSRSVSSTTM 457
Score = 90.0 bits (190), Expect(2) = 2e-046
Identities = 44/72 (61%), Positives = 47/72 (65%)
Frame = -2 / -1
Query: 708 FASIIRTSLKPGYWQKSIWSIMGVVSCGKFWTNKMLFGTIISGIMTLCPPAIGIWVSCGA 529
FASIIRTSL GY K+I SI V SCG WT ++LFGTIISGIMT G S GA
Sbjct: 683 FASIIRTSLNHGYC*KNICSIKAVASCGSSWTKRILFGTIISGIMTFGTAPKGS*ESGGA 504
Query: 528 P*TPG*AVTPMP 493
P* PG*A P P
Sbjct: 503 P*APG*ACMPTP 468
Score = 78.0 bits (164), Expect(3) = 7e-052
Identities = 33/77 (42%), Positives = 41/77 (53%)
Frame = +1 / +3
Query: 481 C*SNWHGCNCLPWCLWGSTAHPDTYSRRTKGHDARDYRTKQHLVCPKLATRXXXXXXXXX 660
C* W G CL WCLWGS A T+ T+ HDAR Y TKQ+ + P+ TR
Sbjct: 456 C*PGWRGHACLSWCLWGSAALSATFWCGTESHDARYYCTKQYSLRPRTPTRRDRFDAADV 635
Query: 661 XXXVPWLQGGSDDRGEA 711
VP +Q +DDR +A
Sbjct: 636 LLAVPMVQRSADDRSKA 686
Score = 73.5 bits (154), Expect(3) = 3e-041
Identities = 35/44 (79%), Positives = 38/44 (86%)
Frame = -2 / -1
Query: 411 RGTKVPSAFAIISDLVLAY*ILIVLS*KGKPCILFRASVADATS 280
RGTKVPSAFA DLVLAY*I + LS*KGK CILF+ASVA+ATS
Sbjct: 392 RGTKVPSAFATTFDLVLAY*IFMGLS*KGK*CILFKASVANATS 261
Score = 30.4 bits (60), Expect(3) = 3e-041
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = -2 / -3
Query: 258 PLCFKVLKASTSRIFPNWEKTA 193
PLC +VLKAS S +FP + A
Sbjct: 240 PLCLEVLKASPSMLFPTAREPA 175
Score = 28.1 bits (55), Expect(2) = 2e-021
Identities = 16/32 (50%), Positives = 17/32 (53%)
Frame = +3 / +2
Query: 525 MGLHSSPRYL*QEDKGS*CQRLSYQTTSCLSK 620
MGL S YL + S*CQ L YQT SK
Sbjct: 500 MGLRRSLSYLLVRYRKS*CQILLYQTVFSSSK 595
Score = 26.3 bits (51), Expect(3) = 7e-052
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = +2 / +2
Query: 212 GKILDVLAFKTLKHKG 259
G ++ LAFKT KHKG
Sbjct: 194 GNSIEGLAFKTSKHKG 241
>gb|AL501018.1|AL501018 AL501018 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW02L23u 3', mRNA sequence
Length = 658
Score = 193 bits (416), Expect(2) = 7e-052
Identities = 78/101 (77%), Positives = 86/101 (85%)
Frame = +2 / -2
Query: 485 SQIGMGVTAYPGVYGAPQLTQIPIAGGQRVMMPEIIVPNNILFVQNLPHETTPMMLQMLF 664
+Q G+G+ AYPG YGAP L+Q+P G +VMMPEIIVPNNILFVQ LPH+ T +MLQM F
Sbjct: 594 TQAGVGMHAYPGAYGAPPLSQLPFGAGPKVMMPEIIVPNNILFVQELPHDATALMLQMFF 415
Query: 665 CQYPGFKEVRMIEAKPGIAFVEYGDETQATAAMNNLQSFKI 787
CQYPGFKEVRMIEAKPGIAFVEYGDE QATAAMN LQ FKI
Sbjct: 414 CQYPGFKEVRMIEAKPGIAFVEYGDEGQATAAMNLLQGFKI 292
Score = 151 bits (324), Expect(2) = 5e-039
Identities = 70/99 (70%), Positives = 74/99 (74%)
Frame = -2 / +2
Query: 789 VILKL*RLFMAAVAWVSSPYSTKAIPGFASIIRTSLKPGYWQKSIWSIMGVVSCGKFWTN 610
+ILK *R FMAAVA SSPYSTKAIPGFASIIRTSL PGYWQK+I SI V SCG WT
Sbjct: 290 LILKP*RRFMAAVACPSSPYSTKAIPGFASIIRTSLNPGYWQKNICSIKAVASCGSSWTK 469
Query: 609 KMLFGTIISGIMTLCPPAIGIWVSCGAP*TPG*AVTPMP 493
++LFGTIISGIMT P G S GAP* PG*A P P
Sbjct: 470 RILFGTIISGIMTFGPAPKGS*ESGGAP*APG*ACMPTP 586
Score = 140 bits (301), Expect = 1e-032
Identities = 57/100 (57%), Positives = 72/100 (72%)
Frame = -1 / +1
Query: 787 NFEALKVVHGSSGLGLVSILHESNPRLRLDHPNLLEARVLAEEHLEHHGRRLVWQVLDKQ 608
+FE LK VHG L LVSILH+SN RL DHP+ LE VLAEEHL+H R+VW+ LD++
Sbjct: 292 DFETLKKVHGCGRLSLVSILHKSNTRLCFDHPHFLEPWVLAEEHLQHQSGRVVWEFLDEE 471
Query: 607 DVVWYDNLWHHDPLSSCYRYLGELWSPINTRVSSYTHANL 488
++VWY+NLWHHD S R L E SPI+TR+ + HA+L
Sbjct: 472 NIVWYNNLWHHDLRSRTKR*LRERRSPISTRIGMHAHASL 591
Score = 117 bits (250), Expect = 1e-025
Identities = 49/106 (46%), Positives = 58/106 (54%)
Frame = +1 / -1
Query: 493 WHGCNCLPWCLWGSTAHPDTYSRRTKGHDARDYRTKQHLVCPKLATRXXXXXXXXXXXXV 672
W G CL WCLWGS A T+ T+GHDARDY TKQ+ + P+ TR V
Sbjct: 586 WRGHACLSWCLWGSAALSATFWCGTEGHDARDYCTKQYSLRPRTPTRRDRFDAADVLLPV 407
Query: 673 PWLQGGSDDRGEAWDCFRGVWRRDPGHCCHEQPSKLQNYKRKPDGH 810
P +QG +DDR +AW CF GVWRR G HE S QN + H
Sbjct: 406 PRVQGSADDRSKAWYCFCGVWRRGTGDRSHEPSSGFQNQREPNAHH 269
Score = 31.3 bits (62), Expect(2) = 7e-052
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +1 / -1
Query: 766 QPSKLQNYKRKPDGHHICEEV 828
+PS +R+P+ HHICEEV
Sbjct: 316 EPSSGFQNQREPNAHHICEEV 254
Score = 30.4 bits (60), Expect(2) = 5e-039
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = -2 / +2
Query: 876 RNTTSHTSGKHCSGAHYFFAYVMTIWFSFVI 784
+N + + S + YFFAYVM I FS ++
Sbjct: 206 QNQGYNINSSQYSSSSYFFAYVMRIGFSLIL 298
>gb|CA001055.1|CA001055 HS05J13u HS Hordeum vulgare subsp. vulgare cDNA clone HS05J13
3-PRIME, mRNA sequence
Length = 502
Score = 193 bits (415), Expect(2) = 1e-051
Identities = 78/111 (70%), Positives = 88/111 (79%)
Frame = +2 / -3
Query: 455 KREHHHDAGASQIGMGVTAYPGVYGAPQLTQIPIAGGQRVMMPEIIVPNNILFVQNLPHE 634
K++ +Q G+G+ AYPG YG P L+Q+P G +VMMPEIIVPNNILFVQ LPH+
Sbjct: 500 KQKREQHHDVNQAGVGMHAYPGAYGXPPLSQLPFGAGPKVMMPEIIVPNNILFVQELPHD 321
Query: 635 TTPMMLQMLFCQYPGFKEVRMIEAKPGIAFVEYGDETQATAAMNNLQSFKI 787
T +MLQM FCQYPGFKEVRMIEAKPGIAFVEYGDE QATAAMN LQ FKI
Sbjct: 320 ATALMLQMFFCQYPGFKEVRMIEAKPGIAFVEYGDEGQATAAMNLLQGFKI 168
Score = 149 bits (320), Expect(2) = 2e-038
Identities = 69/99 (69%), Positives = 73/99 (73%)
Frame = -2 / +1
Query: 789 VILKL*RLFMAAVAWVSSPYSTKAIPGFASIIRTSLKPGYWQKSIWSIMGVVSCGKFWTN 610
+ILK *R FMAAVA SSPYSTKAIPGFASIIRTSL PGYWQK+I SI V SCG WT
Sbjct: 166 LILKP*RRFMAAVACPSSPYSTKAIPGFASIIRTSLNPGYWQKNICSIKAVASCGSSWTK 345
Query: 609 KMLFGTIISGIMTLCPPAIGIWVSCGAP*TPG*AVTPMP 493
++LFGTIISGIMT P G S G P* PG*A P P
Sbjct: 346 RILFGTIISGIMTFGPAPKGS*ESGGXP*APG*ACMPTP 462
Score = 143 bits (307), Expect = 2e-033
Identities = 58/111 (52%), Positives = 77/111 (69%)
Frame = -1 / +3
Query: 787 NFEALKVVHGSSGLGLVSILHESNPRLRLDHPNLLEARVLAEEHLEHHGRRLVWQVLDKQ 608
+FE LK VHG L LVSILH+SN RL DHP+ LE VLAEEHL+H R+VW+ LD++
Sbjct: 168 DFETLKKVHGCGRLSLVSILHKSNTRLCFDHPHFLEPWVLAEEHLQHQSGRVVWEFLDEE 347
Query: 607 DVVWYDNLWHHDPLSSCYRYLGELWSPINTRVSSYTHANLTSTSIMVMLAL 455
++VWY+NLWHHD S R L E PI+TR+ + HA+L + +++ L L
Sbjct: 348 NIVWYNNLWHHDLRSRTKR*LRERRXPISTRIGMHAHASLVNIVVLLTLLL 500
Score = 121 bits (259), Expect(2) = 5e-030
Identities = 51/110 (46%), Positives = 60/110 (54%)
Frame = +1 / -2
Query: 481 C*SNWHGCNCLPWCLWGSTAHPDTYSRRTKGHDARDYRTKQHLVCPKLATRXXXXXXXXX 660
C* W G CL WCLWGS A T+ T+GHDARDY TKQ+ + P+ TR
Sbjct: 474 C*PGWRGHACLSWCLWGSAALSATFWCGTEGHDARDYCTKQYSLRPRTPTRRDRFDAADV 295
Query: 661 XXXVPWLQGGSDDRGEAWDCFRGVWRRDPGHCCHEQPSKLQNYKRKPDGH 810
VP +QG +DDR +AW CF GVWRR G HE S QN + H
Sbjct: 294 LLPVPRVQGSADDRSKAWYCFCGVWRRGTGDRSHEPSSGFQNQREPNAHH 145
Score = 31.3 bits (62), Expect(2) = 1e-051
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +1 / -2
Query: 766 QPSKLQNYKRKPDGHHICEEV 828
+PS +R+P+ HHICEEV
Sbjct: 192 EPSSGFQNQREPNAHHICEEV 130
Score = 30.4 bits (60), Expect(2) = 2e-038
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = -2 / +1
Query: 876 RNTTSHTSGKHCSGAHYFFAYVMTIWFSFVI 784
+N + + S + YFFAYVM I FS ++
Sbjct: 82 QNQGYNINSSQYSSSSYFFAYVMRIGFSLIL 174
Score = 29.9 bits (59), Expect(2) = 5e-030
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +2 / -3
Query: 449 KQKREHHHDAGASQIGM 499
KQKRE HHD + +GM
Sbjct: 500 KQKREQHHDVNQAGVGM 450
>gb|BM368467.2|BM368467 EBem08_SQ002_G06_R embryo, 40 DPA, no treatment, cv Optic, EBem08
Hordeum vulgare subsp. vulgare cDNA clone
EBem08_SQ002_G06 5', mRNA sequence
Length = 505
Score = 132 bits (282), Expect(2) = 2e-046
Identities = 57/81 (70%), Positives = 65/81 (80%)
Frame = +2 / +1
Query: 596 PNNILFVQNLPHETTPMMLQMLFCQYPGFKEVRMIEAKPGIAFVEYGDETQATAAMNNLQ 775
PNNILF+QNLP +TT +MLQ+LF QYPGF+EVRMIEAKPGIAFVEY DE Q+ AM LQ
Sbjct: 259 PNNILFLQNLPDQTTSVMLQILFQQYPGFREVRMIEAKPGIAFVEYEDENQSMVAMEALQ 438
Query: 776 SFKITKENQMVITYAKK**AP 838
FKI+ N M I+YAKK* P
Sbjct: 439 GFKISP*NPMAISYAKK*TIP 501
Score = 77.1 bits (162), Expect(2) = 2e-046
Identities = 28/49 (57%), Positives = 40/49 (81%)
Frame = +2 / +1
Query: 311 RMQGFPFYDKTMRIQYAKTKSDIIAKADGTFVPRERRKRTDEKSEKKQK 457
R +GF FY K MR+QYAKTKSD AKADG++ P+E+RK+ +EK+ +K++
Sbjct: 1 RTRGFDFYGKKMRVQYAKTKSDCFAKADGSYAPKEKRKKQEEKAAEKKR 147
>gb|CA025379.1|CA025379 HZ51P24r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ51P24
5-PRIME, mRNA sequence
Length = 631
Score = 139 bits (298), Expect(2) = 3e-046
Identities = 55/115 (47%), Positives = 79/115 (68%)
Frame = +2 / +1
Query: 116 IPPNVTIYXXXXXXXXXXXXXXXSLTAVFSQFGKILDVLAFKTLKHKGQAWVVFEDVASA 295
+PPN T+Y SL A+ SQ+G+I+DV+A KT K +GQAWV F ++ +A
Sbjct: 73 VPPNQTVYFKNLNEKVKKEELKRSLYALCSQYGRIVDVVALKTHKLRGQAWVAFSEITAA 252
Query: 296 TEALKRMQGFPFYDKTMRIQYAKTKSDIIAKADGTFVPRERRKRTDEKSEKKQKR 460
T A + +Q F FY K MR+QYAKTKSD AKADG++ P+E+RK+ +EK+ +K++R
Sbjct: 253 TNAFRGLQDFDFYGKKMRVQYAKTKSDCFAKADGSYAPKEKRKKQEEKAAEKKRR 417
Score = 69.3 bits (145), Expect(2) = 3e-046
Identities = 27/35 (77%), Positives = 32/35 (91%)
Frame = +2 / +1
Query: 596 PNNILFVQNLPHETTPMMLQMLFCQYPGFKEVRMI 700
PNNILF+QNLP +TT +MLQ+LF QYPGF+EVRMI
Sbjct: 526 PNNILFLQNLPDQTTSVMLQILFQQYPGFREVRMI 630
>gb|AV920834.1|AV920834 AV920834 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags15m05 3', mRNA sequence
Length = 560
Score = 136 bits (291), Expect(2) = 5e-044
Identities = 58/81 (71%), Positives = 66/81 (81%)
Frame = +2 / -1
Query: 596 PNNILFVQNLPHETTPMMLQMLFCQYPGFKEVRMIEAKPGIAFVEYGDETQATAAMNNLQ 775
PNNILF+QNLP +TT +MLQ+LF QYPGF+EVRMIEAKPGIAFVEY DE Q+ AM LQ
Sbjct: 332 PNNILFLQNLPDQTTSVMLQILFQQYPGFREVRMIEAKPGIAFVEYEDENQSMVAMEALQ 153
Query: 776 SFKITKENQMVITYAKK**AP 838
FKI+ EN M I+YAKK* P
Sbjct: 152 GFKISPENPMAISYAKK*TIP 90
Score = 102 bits (217), Expect = 4e-021
Identities = 47/81 (58%), Positives = 54/81 (66%)
Frame = -2 / +1
Query: 837 GAHYFFAYVMTIWFSFVILKL*RLFMAAVAWVSSPYSTKAIPGFASIIRTSLKPGYWQKS 658
G +F AY + I FS +ILK R +A + W SS YSTKA+PG ASIIRTS KPGY
Sbjct: 91 GIVHFLAYDIAIGFSGLILKPWRASIATMDWFSSSYSTKAMPGLASIIRTSRKPGYCWNR 270
Query: 657 IWSIMGVVSCGKFWTNKMLFG 595
IWSI VV GKFW +MLFG
Sbjct: 271 IWSITLVVWSGKFWRKRMLFG 333
Score = 64.8 bits (135), Expect(2) = 5e-044
Identities = 23/39 (58%), Positives = 34/39 (87%)
Frame = +2 / -1
Query: 344 MRIQYAKTKSDIIAKADGTFVPRERRKRTDEKSEKKQKR 460
MR+QYAKTKSD AKADG++ P+E+RK+ +EK+ +K++R
Sbjct: 557 MRVQYAKTKSDCFAKADGSYAPKEKRKKQEEKAAEKKRR 441
>gb|BU974881.1|BU974881 HB29G07r BC Hordeum vulgare subsp. vulgare cDNA clone HB29G07
5-PRIME, mRNA sequence
Length = 410
Score = 173 bits (372), Expect = 2e-042
Identities = 73/98 (74%), Positives = 79/98 (80%)
Frame = +2 / +2
Query: 116 IPPNVTIYXXXXXXXXXXXXXXXSLTAVFSQFGKILDVLAFKTLKHKGQAWVVFEDVASA 295
+ PNVTIY SL AVFSQFGKI++VLAFKTLKHKGQAWVVFEDVASA
Sbjct: 116 VQPNVTIYINNLNEKIKLDALKKSLNAVFSQFGKIIEVLAFKTLKHKGQAWVVFEDVASA 295
Query: 296 TEALKRMQGFPFYDKTMRIQYAKTKSDIIAKADGTFVP 409
TEALKRMQ FPFYDK M+IQYAKTKSD++AKADGTFVP
Sbjct: 296 TEALKRMQDFPFYDKPMKIQYAKTKSDVVAKADGTFVP 409
Score = 146 bits (314), Expect = 2e-034
Identities = 65/106 (61%), Positives = 76/106 (71%)
Frame = -1 / -2
Query: 409 RYKGAISLCYNI*FGLGILNSHSFVIEGKALHPLQSLSS*CDIFEDNPSLTFMLQSLESK 230
RY+GAIS CYNI* GLGILN H FVIEG+ LHPLQSL *C+IFE +P L FMLQSL+SK
Sbjct: 409 RYEGAISFCYNI*LGLGILNFHGFVIEGEILHPLQSLGC*CNIFEHDPCLPFMLQSLKSK 230
Query: 229 HIKNFPXXXXXXXXXXXKFFELDLLVEVVDIDGDVRRDAIRLSFAS 92
H+ +FP K E DLLVEV+D+DG V DA+RL A+
Sbjct: 229 HLNDFPKLGENRIEGLLKCVEFDLLVEVIDVDGHVWLDAVRLPAAA 92
Score = 146 bits (314), Expect = 2e-034
Identities = 69/96 (71%), Positives = 72/96 (75%)
Frame = -2 / -3
Query: 408 GTKVPSAFAIISDLVLAY*ILIVLS*KGKPCILFRASVADATSSKTTQA*PLCFKVLKAS 229
GTKVPSAFA SDLVLAY*I + LS*KGK CILF+ASVADATSS TT A PLCFKVLKAS
Sbjct: 408 GTKVPSAFATTSDLVLAY*IFMGLS*KGKSCILFKASVADATSSNTTHACPLCFKVLKAS 229
Query: 228 TSRIFPNWEKTAVRDXXXXXXXXXXXXXXX*MVTFG 121
TS IFPNWEKTA+RD *MVTFG
Sbjct: 228 TSMIFPNWEKTALRDFLSASSLIFSLRLLM*MVTFG 121
Score = 141 bits (302), Expect = 8e-033
Identities = 68/98 (69%), Positives = 76/98 (77%)
Frame = -3 / -1
Query: 410 EVQRCHQPLL*YLIWSWHIEFS*FCHRRESPASSSEPQ*LMRHLRRQPKLDLYASKS*KQ 231
EV+RCHQ LL +L WSWHIEFS CHRR + ASSS+P+ LM+HLR +P L LYASKS*KQ
Sbjct: 410 EVRRCHQLLLQHLTWSWHIEFSWVCHRRGNLASSSKPRLLMQHLRTRPMLALYASKS*KQ 231
Query: 230 AHQEFSQTGKKRLLGTS*VLRA*SSR*GC*YRW*RSAG 117
A Q FSQTG+K GTS*V R *SSR*G * RW R AG
Sbjct: 230 APQ*FSQTGRKPH*GTS*VRRV*SSR*GY*CRWSRLAG 117
Score = 127 bits (272), Expect = 1e-028
Identities = 60/97 (61%), Positives = 64/97 (65%)
Frame = +1 / +1
Query: 118 PAERHHLYQQPQREDQARRT*EVPNSRFFPVWENS*CACFQDFEAXXXXXXXXXXXXISY 297
PA+R HL+Q PQREDQ RRT*EVP F PVWEN * ACF DFEA IS
Sbjct: 118 PAKRDHLHQ*PQREDQTRRT*EVPQCGFLPVWENH*GACF*DFEA*RASMGRVRRCCISN 297
Query: 298 *GSEEDAGLSLL*QNYENSICQDQIRYYSKG*WHLCT 408
G EEDA LL*Q +ENSICQDQ+R SK *WHL T
Sbjct: 298 RGFEEDARFPLL*QTHENSICQDQVRCCSKS*WHLRT 408
Score = 112 bits (240), Expect = 3e-024
Identities = 57/102 (55%), Positives = 63/102 (61%)
Frame = +3 / +3
Query: 105 RRMAXXXXXXXXXXXXXXXXXXKNLRSP*QPFFPSLGKFLMCLLSRL*SIKVKLGLSSKM 284
RR A +LRSP F PSLGK L CLL RL*SIK K G SKM
Sbjct: 105 RRTASSQT*PSTSITSTRRSNSTHLRSPSMRFSPSLGKSLRCLLLRL*SIKGKHGSCSKM 284
Query: 285 SHQLLRL*RGCRAFPSMTKL*EFNMPRPNQIL*QRLMAPLYL 410
HQ RL*RGC+ PSMT *+FNMPRP+Q+L*Q+LMAP YL
Sbjct: 285 LHQQPRL*RGCKISPSMTNP*KFNMPRPSQML*QKLMAPSYL 410
>gb|BM097565.2|BM097565 EBem04_SQ002_B01_R embryo, 12 DPA, no treatment, cv Optic, EBem04
Hordeum vulgare subsp. vulgare cDNA clone
EBem04_SQ002_B01 5', mRNA sequence
Length = 581
Score = 139 bits (298), Expect(2) = 1e-037
Identities = 55/115 (47%), Positives = 79/115 (68%)
Frame = +2 / +3
Query: 116 IPPNVTIYXXXXXXXXXXXXXXXSLTAVFSQFGKILDVLAFKTLKHKGQAWVVFEDVASA 295
+PPN T+Y SL A+ SQ+G+I+DV+A KT K +GQAWV F ++ +A
Sbjct: 69 VPPNQTVYFKNLNEKVKKEELKRSLYALCSQYGRIVDVVALKTHKLRGQAWVAFSEITAA 248
Query: 296 TEALKRMQGFPFYDKTMRIQYAKTKSDIIAKADGTFVPRERRKRTDEKSEKKQKR 460
T A + +Q F FY K MR+QYAKTKSD AKADG++ P+E+RK+ +EK+ +K++R
Sbjct: 249 TNAFRGLQDFDFYGKKMRVQYAKTKSDCFAKADGSYAPKEKRKKQEEKAAEKKRR 413
Score = 40.0 bits (81), Expect(2) = 1e-037
Identities = 15/20 (75%), Positives = 18/20 (90%)
Frame = +2 / +3
Query: 596 PNNILFVQNLPHETTPMMLQ 655
PNNILF+QNLP +TT +MLQ
Sbjct: 522 PNNILFLQNLPDQTTSVMLQ 581
>gb|BG299708.1|BG299708 HVSMEa0021H20f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0021H20f, mRNA sequence
Length = 799
Score = 132 bits (283), Expect(2) = 4e-037
Identities = 56/83 (67%), Positives = 61/83 (73%)
Frame = +2 / +1
Query: 116 IPPNVTIYXXXXXXXXXXXXXXXSLTAVFSQFGKILDVLAFKTLKHKGQAWVVFEDVASA 295
+ PNVTIY SL AVF QFGKI++VLAFKTLKHKGQAWVVFEDVASA
Sbjct: 100 VQPNVTIYINNLNEKIKLDALKKSLNAVFCQFGKIIEVLAFKTLKHKGQAWVVFEDVASA 279
Query: 296 TEALKRMQGFPFYDKTMRIQYAK 364
TEA KRMQ FPFYDK MRI+Y +
Sbjct: 280 TEAFKRMQDFPFYDKPMRIEYGR 348
Score = 112 bits (239), Expect(2) = 2e-027
Identities = 50/89 (56%), Positives = 61/89 (68%)
Frame = -1 / -2
Query: 358 ILNSHSFVIEGKALHPLQSLSS*CDIFEDNPSLTFMLQSLESKHIKNFPXXXXXXXXXXX 179
I NSH FVIEG+ LHPL+SL *C+IFE +P L FMLQSL+SKH+ +FP
Sbjct: 342 IFNSHGFVIEGEILHPLKSLGC*CNIFEHDPCLPFMLQSLKSKHLNDFPKLAENRIEGLL 163
Query: 178 KFFELDLLVEVVDIDGDVRRDAIRLSFAS 92
K E DLLVEV+D+DG V DA+RL A+
Sbjct: 162 KCVEFDLLVEVIDVDGHVWLDAVRLPAAA 76
Score = 110 bits (235), Expect(2) = 2e-026
Identities = 52/79 (65%), Positives = 56/79 (70%)
Frame = -2 / -3
Query: 357 Y*ILIVLS*KGKPCILFRASVADATSSKTTQA*PLCFKVLKASTSRIFPNWEKTAVRDXX 178
Y IL+ LS*KGK CIL +ASVADATSS TT A PLCFKVLKASTS IFPNW+KTA+RD
Sbjct: 341 YSILMGLS*KGKSCILLKASVADATSSNTTHACPLCFKVLKASTSMIFPNWQKTALRDFL 162
Query: 177 XXXXXXXXXXXXX*MVTFG 121
*MVTFG
Sbjct: 161 SASSLIFSLRLLM*MVTFG 105
Score = 104 bits (222), Expect(2) = 3e-024
Identities = 54/81 (66%), Positives = 61/81 (75%)
Frame = -3 / -1
Query: 359 HIEFS*FCHRRESPASSSEPQ*LMRHLRRQPKLDLYASKS*KQAHQEFSQTGKKRLLGTS 180
HI+FS CHRR + ASS +P+ LM+HLR +P L LYASKS*KQA Q FSQTG+K GTS
Sbjct: 343 HIQFSWVCHRRGNLASS*KPRLLMQHLRTRPMLALYASKS*KQAPQ*FSQTGRKPH*GTS 164
Query: 179 *VLRA*SSR*GC*YRW*RSAG 117
*V R *SSR*G * RW R AG
Sbjct: 163 *VRRV*SSR*GY*CRWSRLAG 101
Score = 90.4 bits (191), Expect(2) = 2e-022
Identities = 45/78 (57%), Positives = 48/78 (61%)
Frame = +1 / +3
Query: 118 PAERHHLYQQPQREDQARRT*EVPNSRFFPVWENS*CACFQDFEAXXXXXXXXXXXXISY 297
PA+R HL+Q PQREDQ RRT*EVP F PVWEN * ACF DFEA IS
Sbjct: 102 PAKRDHLHQ*PQREDQTRRT*EVPQCGFLPVWENH*GACF*DFEA*RASMGRVRRCCISN 281
Query: 298 *GSEEDAGLSLL*QNYEN 351
G EDA LL*Q +EN
Sbjct: 282 RGF*EDARFPLL*QTHEN 335
Score = 42.8 bits (87), Expect(2) = 4e-037
Identities = 17/40 (42%), Positives = 27/40 (67%)
Frame = +2 / +2
Query: 350 IQYAKTKSDIIAKADGTFVPRERRKRTDEKSEKKQKREHH 469
+ A+TKSD+++KADGTFVP E+ R ++ ++ HH
Sbjct: 335 LNMAETKSDVVSKADGTFVPPEKGTRR*YT*KESKR*AHH 454
Score = 35.4 bits (71), Expect(2) = 2e-022
Identities = 13/21 (61%), Positives = 19/21 (90%)
Frame = +3 / +3
Query: 360 PRPNQIL*QRLMAPLYLEKEE 422
PRP+Q+L Q+LMAP +L+K+E
Sbjct: 345 PRPSQMLYQKLMAPSFLQKKE 407
Score = 30.9 bits (61), Expect(2) = 2e-027
Identities = 18/37 (48%), Positives = 21/37 (56%)
Frame = -1 / -1
Query: 469 VMLALLLLFRFFISPLSSFSRYKGAISLCYNI*FGLG 359
V+ + L F S S F R +GAIS YNI* GLG
Sbjct: 454 VVSSTL*FFSGVSSSCSFFWRNEGAISF*YNI*LGLG 344
Score = 29.0 bits (57), Expect(2) = 2e-026
Identities = 13/17 (76%), Positives = 13/17 (76%)
Frame = -2 / -2
Query: 408 GTKVPSAFAIISDLVLA 358
GTKVPSAF SDLV A
Sbjct: 393 GTKVPSAFDTTSDLVSA 343
Score = 27.6 bits (54), Expect(2) = 3e-024
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = -3 / -3
Query: 413 LEVQRCHQPLL*YLIWS 363
LE +RCHQ L+ +L WS
Sbjct: 398 LEERRCHQLLIQHLTWS 348
>gb|AL505772.1|AL505772 AL505772 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW09H18V 5', mRNA sequence
Length = 500
Score = 139 bits (298), Expect = 3e-032
Identities = 55/115 (47%), Positives = 79/115 (68%)
Frame = +2 / +2
Query: 116 IPPNVTIYXXXXXXXXXXXXXXXSLTAVFSQFGKILDVLAFKTLKHKGQAWVVFEDVASA 295
+PPN T+Y SL A+ SQ+G+I+DV+A KT K +GQAWV F ++ +A
Sbjct: 65 VPPNQTVYFKNLNEKVKKEELKRSLYALCSQYGRIVDVVALKTHKLRGQAWVAFSEITAA 244
Query: 296 TEALKRMQGFPFYDKTMRIQYAKTKSDIIAKADGTFVPRERRKRTDEKSEKKQKR 460
T A + +Q F FY K MR+QYAKTKSD AKADG++ P+E+RK+ +EK+ +K++R
Sbjct: 245 TNAFRGLQDFDFYGKKMRVQYAKTKSDCFAKADGSYAPKEKRKKQEEKAAEKKRR 409
>gb|AV915725.1|AV915725 AV915725 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags15m05 5', mRNA sequence
Length = 510
Score = 139 bits (298), Expect = 3e-032
Identities = 55/115 (47%), Positives = 79/115 (68%)
Frame = +2 / +3
Query: 116 IPPNVTIYXXXXXXXXXXXXXXXSLTAVFSQFGKILDVLAFKTLKHKGQAWVVFEDVASA 295
+PPN T+Y SL A+ SQ+G+I+DV+A KT K +GQAWV F ++ +A
Sbjct: 75 VPPNQTVYFKNLNEKVKKEELKRSLYALCSQYGRIVDVVALKTHKLRGQAWVAFSEITAA 254
Query: 296 TEALKRMQGFPFYDKTMRIQYAKTKSDIIAKADGTFVPRERRKRTDEKSEKKQKR 460
T A + +Q F FY K MR+QYAKTKSD AKADG++ P+E+RK+ +EK+ +K++R
Sbjct: 255 TNAFRGLQDFDFYGKKMRVQYAKTKSDCFAKADGSYAPKEKRKKQEEKAAEKKRR 419
>gb|CA000585.1|CA000585 HS08C04u HS Hordeum vulgare subsp. vulgare cDNA clone HS08C04
3-PRIME, mRNA sequence
Length = 468
Score = 136 bits (291), Expect = 3e-031
Identities = 58/81 (71%), Positives = 66/81 (81%)
Frame = +2 / -3
Query: 596 PNNILFVQNLPHETTPMMLQMLFCQYPGFKEVRMIEAKPGIAFVEYGDETQATAAMNNLQ 775
PNNILF+QNLP +TT +MLQ+LF QYPGF+EVRMIEAKPGIAFVEY DE Q+ AM LQ
Sbjct: 382 PNNILFLQNLPDQTTSVMLQILFQQYPGFREVRMIEAKPGIAFVEYEDENQSMVAMEALQ 203
Query: 776 SFKITKENQMVITYAKK**AP 838
FKI+ EN M I+YAKK* P
Sbjct: 202 GFKISPENPMAISYAKK*TIP 140
Score = 102 bits (217), Expect = 4e-021
Identities = 47/81 (58%), Positives = 54/81 (66%)
Frame = -2 / +3
Query: 837 GAHYFFAYVMTIWFSFVILKL*RLFMAAVAWVSSPYSTKAIPGFASIIRTSLKPGYWQKS 658
G +F AY + I FS +ILK R +A + W SS YSTKA+PG ASIIRTS KPGY
Sbjct: 141 GIVHFLAYDIAIGFSGLILKPWRASIATMDWFSSSYSTKAMPGLASIIRTSRKPGYCWNR 320
Query: 657 IWSIMGVVSCGKFWTNKMLFG 595
IWSI VV GKFW +MLFG
Sbjct: 321 IWSITLVVWSGKFWRKRMLFG 383
>gb|BQ761571.1|BQ761571 EBem10_SQ001_K04_R embryo, 2 Day germination, no treatment, cv
Optic, EBem10 Hordeum vulgare subsp. vulgare cDNA clone
EBem10_SQ001_K04 5', mRNA sequence
Length = 342
Score = 115 bits (245), Expect = 6e-025
Identities = 47/96 (48%), Positives = 63/96 (65%)
Frame = +2 / +2
Query: 116 IPPNVTIYXXXXXXXXXXXXXXXSLTAVFSQFGKILDVLAFKTLKHKGQAWVVFEDVASA 295
+PPN T+Y SL A+ SQ+G+I+DV+A KT K +GQAWV F ++ +A
Sbjct: 50 VPPNQTVYFKNLNEKVKKEELKRSLYALCSQYGRIVDVVALKTHKLRGQAWVAFSEITAA 229
Query: 296 TEALKRMQGFPFYDKTMRIQYAKTKSDIIAKADGTF 403
T A + +Q F FY K MR+QYAKTKSD AKADG++
Sbjct: 230 TNAFRGLQDFDFYGKKMRVQYAKTKSDCFAKADGSY 337
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 429,623,375
Number of Sequences: 312970
Number of extensions: 6605402
Number of successful extensions: 43618
Number of sequences better than 1.0e-020: 38
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 12996929159
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)