TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2969966.2.1
(1029 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA022161.1|CA022161 HZ42F09r HZ Hordeum vulgare subsp. v... 317 2e-097
gb|BQ472196.1|BQ472196 HV04L02r HV Hordeum vulgare subsp. v... 270 9e-072
gb|CA009554.1|CA009554 HU14H09r HU Hordeum vulgare subsp. v... 270 9e-072
gb|AV934271.1|AV934271 AV934271 K. Sato unpublished cDNA li... 113 6e-043
gb|BF621467.2|BF621467 HVSMEa0011B10f Hordeum vulgare seedl... 137 6e-034
gb|CA030942.1|CA030942 HX08J07r HX Hordeum vulgare subsp. v... 121 2e-032
gb|CA032804.1|CA032804 HX14E02r HX Hordeum vulgare subsp. v... 71 3e-030
gb|BU987774.1|BU987774 HF15L16r HF Hordeum vulgare subsp. v... 71 3e-030
gb|AV933277.1|AV933277 AV933277 K. Sato unpublished cDNA li... 121 8e-027
gb|BJ472357.1|BJ472357 BJ472357 K. Sato unpublished cDNA li... 64 3e-025
gb|CA008952.1|CA008952 HU12K18r HU Hordeum vulgare subsp. v... 112 3e-024
>gb|CA022161.1|CA022161 HZ42F09r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ42F09
5-PRIME, mRNA sequence
Length = 576
Score = 317 bits (686), Expect(2) = 2e-097
Identities = 124/134 (92%), Positives = 128/134 (95%)
Frame = +3 / +1
Query: 336 RMLFETEESSVRRYMGTITEVSDADPVRWPSSYWRSVKVGWDESTAGERPPRVSLWEIEP 515
RMLFETEESSVRRYMGTITEVSDADPVRW SSYWRSVKVGWDESTAGERPPRVSLWEIEP
Sbjct: 1 RMLFETEESSVRRYMGTITEVSDADPVRWASSYWRSVKVGWDESTAGERPPRVSLWEIEP 180
Query: 516 LTTFPMYPSLFPLRVKHPWYSGLAALHDDSNALMWLRGVTGEGGFHSSNFQSPGIGSWGQ 695
LTTFPMYPSLFPLRVKHPWYSG+A L DDSNALMWLRGV G+GG+ S NFQSPGIGSWGQ
Sbjct: 181 LTTFPMYPSLFPLRVKHPWYSGVAGLQDDSNALMWLRGVAGDGGYQSMNFQSPGIGSWGQ 360
Query: 696 QRLHPSLLSNDHDQ 737
QRLHPSLLS+DHDQ
Sbjct: 361 QRLHPSLLSSDHDQ 402
Score = 273 bits (591), Expect(2) = 2e-077
Identities = 116/134 (86%), Positives = 125/134 (93%)
Frame = -3 / -2
Query: 739 Y*SWSLLSKDGWSLCCPQEPIPGDWKFEE*KPPSPVTPLSHIKALLSS*RAASPEYQGCL 560
Y*SWSLLSK+GWSLCCPQEP+PGD KF +* PPSP TPLSHIKALLSS*R A+PEYQGCL
Sbjct: 404 Y*SWSLLSKEGWSLCCPQEPMPGD*KFID**PPSPATPLSHIKALLSS*RPATPEYQGCL 225
Query: 559 TRSGNRDGYIGKVVSGSISHRETLGGLSPAVDSSQPTFTDLQ*ELGQRTGSASLTSVIVP 380
T SGN++GYIGKVV+GSISH+ETLGGLSPAVDSSQPT TDLQ*EL QRTGSASLTSVIVP
Sbjct: 224 TLSGNKEGYIGKVVNGSISHKETLGGLSPAVDSSQPTLTDLQ*ELAQRTGSASLTSVIVP 45
Query: 379 MYLRTLDSSVSNNI 338
MYLRTLDSSVS +I
Sbjct: 44 MYLRTLDSSVSKSI 3
Score = 210 bits (452), Expect = 1e-053
Identities = 90/145 (62%), Positives = 102/145 (70%)
Frame = -2 / -1
Query: 770 GGSCGSNHCLILIMVIAK*GWMESLLSPGTYTR*LEVRRMKAPFTGYSSQPHQSIAVIMK 591
GG SN+CL+LIMV + ME LLSPG R*L+V R+ T SSQPHQSIAVI+K
Sbjct: 435 GGGSSSNNCLVLIMVATQQRRMEPLLSPGANAR*LKVHRLITSITSNSSQPHQSIAVILK 256
Query: 590 SSKS*IPRMLNSQWEQGWIHRKGCQWLNFPQRNSRWPFSCG*FIPTNLH*SPIGTRPTHW 411
+ + IP MLN QW+Q IHRKGCQW N P RNSR F *FIPTNL SP+GT PTHW
Sbjct: 255 ACYTRIPGMLNPQWKQRGIHRKGCQWFNLP*RNSRRSFPSS*FIPTNLDRSPVGTSPTHW 76
Query: 410 VSITHFCYCPHVPADT*FLSLK*HP 336
+ IT FCY PHVPA+T FL LK HP
Sbjct: 75 IGITDFCYRPHVPANTRFLGLKEHP 1
Score = 173 bits (373), Expect = 1e-042
Identities = 85/144 (59%), Positives = 100/144 (69%)
Frame = -1 / -3
Query: 768 RQLRQQPLLDTDHGHC*VRMDGVSVVPRNLYQVTGSSKNESPLHRLLLSATSKHCCHHEE 589
R+ +QQ LL TDHG + DG SVVPR+ QVT SS ++ H+ LLSATSKHCCH E
Sbjct: 433 RRQQQQQLLGTDHGRYSAKKDGASVVPRSQCQVTESS*IDNLHHQQLLSATSKHCCHLEG 254
Query: 588 QQVLNTKDA*LAVGTGMDT*ERLSVAQFPTEKL*VAFLLRLIHPNQPSLISNRN*ANALG 409
NT+DA* +V T DT*ERLS+ Q P +KL* F +LIHPNQP IS+RN* NAL
Sbjct: 253 LLHQNTRDA*PSVETKRDT*ERLSMVQSPIKKL*AVFPQQLIHPNQP*PISSRN*PNALD 74
Query: 408 QHHSLLLLSPCTCGHLIPQSQITS 337
+HH LLL SPCTC H IP+SQ S
Sbjct: 73 RHH*LLLSSPCTCEH*IPRSQRAS 2
Score = 163 bits (351), Expect(2) = 1e-047
Identities = 86/137 (62%), Positives = 90/137 (65%)
Frame = +1 / +2
Query: 337 GCYLRLRNQVSAGTWGQ*QK*VMLTQCVGLVPIGDQ*RLVGMNQPQEKGHLEFLCGKLSH 516
GC LR RN V AGTWG+*QK VM QCVGLVP GD+ RLVGMNQ K LEFL G+L+H
Sbjct: 2 GCSLRPRNLVFAGTWGR*QKSVMPIQCVGLVPTGDRSRLVGMNQLLGKDRLEFLYGRLNH 181
Query: 517 *QPFLCIHPCSHCELSILGIQDLLLFMMTAML*CG*EE*PVKGAFILRTSSHLV*VPGDN 696
*QPFLCI C H LSI GI F MTAML*CG*EE V L T SHL PGDN
Sbjct: 182 *QPFLCIPLCFH*GLSIPGILV*QAFKMTAML*CG*EELLVMEVINL*TFSHLALAPGDN 361
Query: 697 RDSIHPYLAMTMISIKQ 747
R SI TMIS KQ
Sbjct: 362 RGSILLC*VATMISTKQ 412
Score = 158 bits (339), Expect(2) = 2e-042
Identities = 80/124 (64%), Positives = 89/124 (71%)
Frame = +2 / +3
Query: 338 DVI*D*GIKCPQVHGDNNRSE*C*PSALA*FLLEISEGWLG*INRRRKAT*SFSVGN*AT 517
D +*D GI C QVHGD+NRS+*C SAL *FLLEI +GWLG*IN K *SF +G+*
Sbjct: 3 DAL*DRGI*CSQVHGDDNRSQ*CRSSALG*FLLEIGQGWLG*INCWGKTA*SFFMGD*TI 182
Query: 518 DNLSYVSIPVPTAS*ASLVFRTCCSS**QQCFDVAERSNR*RGLSFFELPVTWYRFLGTT 697
DNLSYVS+ V T *ASLVF S *QQCFDVAERS * LS +EL VTW+ LGTT
Sbjct: 183 DNLSYVSLFVSTEG*ASLVFWCSRPSR*QQCFDVAERSCW*WRLSIYELSVTWHWLLGTT 362
Query: 698 ETPS 709
E PS
Sbjct: 363 EAPS 374
Score = 59.7 bits (124), Expect(2) = 2e-097
Identities = 26/46 (56%), Positives = 30/46 (65%)
Frame = +2 / +3
Query: 767 LPVRWLSEAAIPTPSTTYAVPTGAVQSQSAVAATNTTASEPTADG* 904
LP+ WL E AIP PS YAV + +Q Q VAATN AS+PTA *
Sbjct: 435 LPIWWLYETAIPKPSAAYAVASRTLQPQPTVAATNPAASKPTASS* 572
Score = 46.9 bits (96), Expect(2) = 1e-047
Identities = 19/34 (55%), Positives = 26/34 (76%)
Frame = +1 / +2
Query: 805 TFNNLCSPHRSSAISIRCCSNKYYSKRANSRWLV 906
TF++LCS +++A S CCSNK SK+ANS+ LV
Sbjct: 473 TFSSLCSRLKNTATSTHCCSNKSCSKQANSKQLV 574
Score = 36.8 bits (74), Expect(2) = 2e-077
Identities = 20/51 (39%), Positives = 24/51 (47%)
Frame = -1 / -3
Query: 894 AVGSLXXXXXXXXXXXXXXXXVGTA*VVEGVGIAASDNHRTGRQLRQQPLL 742
AVG L TA* EG+GIA S NH+ GR+ +QQ L
Sbjct: 562 AVGLLAAGFVAATVG*GCSVLEATA*AAEGLGIAVSYNHQIGRRRQQQQQL 410
Score = 35.0 bits (70), Expect(2) = 2e-042
Identities = 13/15 (86%), Positives = 15/15 (100%)
Frame = +3 / +1
Query: 786 LKQQFLHLQQPMQSP 830
+KQQFL+LQQPMQSP
Sbjct: 454 MKQQFLNLQQPMQSP 498
>gb|BQ472196.1|BQ472196 HV04L02r HV Hordeum vulgare subsp. vulgare cDNA clone HV04L02
5-PRIME, mRNA sequence
Length = 566
Score = 270 bits (584), Expect = 9e-072
Identities = 109/166 (65%), Positives = 131/166 (78%)
Frame = +3 / +1
Query: 21 TGWSVFVSAKRLVAGDSVLFIWNEKNQLLLGIRHATRPQTVMPSSVLSSDSMHIGLLXXX 200
TGWS+F+S KRL+AGDSVLFI + K QLLLGIR A R T + SSVLSSDSMHIG+L
Sbjct: 31 TGWSLFISGKRLIAGDSVLFIRDAKQQLLLGIRRANRQPTNLSSSVLSSDSMHIGVLAAA 210
Query: 201 XXXXXTNSRFTIFYNPRASPTEFVIPLSKYIKAVFHTRISVGMRFRMLFETEESSVRRYM 380
NS+FTIFYNPRASP+EFVIP +KY KAV+ ++S+GMRFRM+FETEES RRYM
Sbjct: 211 AHAAANNSQFTIFYNPRASPSEFVIPFAKYQKAVYGNQLSLGMRFRMMFETEESGTRRYM 390
Query: 381 GTITEVSDADPVRWPSSYWRSVKVGWDESTAGERPPRVSLWEIEPL 518
GTIT +SD DPVRW +S WR+++V WDE+ ER RVSLW+IEP+
Sbjct: 391 GTITGISDLDPVRWKNSQWRNIQVAWDEAAPSERRTRVSLWDIEPV 528
Score = 155 bits (332), Expect = 5e-037
Identities = 88/172 (51%), Positives = 103/172 (59%)
Frame = -3 / -1
Query: 520 VSGSISHRETLGGLSPAVDSSQPTFTDLQ*ELGQRTGSASLTSVIVPMYLRTLDSSVSNN 341
++GS+S RETL LS SS T *E TGS SL VIVPMYL DSSVSN
Sbjct: 530 MTGSMSQRETLVRLSLGAASSHAT*ILRH*EFFHLTGSRSLMPVIVPMYLLVPDSSVSNI 351
Query: 340 ILNRIPTDIRV*KTALMYFERGITNSVGLALGL*KIVKRLFVXXXXXXXARSPMCMLSLE 161
ILNR+P D + TA Y +GITNS GLALGL* IV L AR+P+CMLSL+
Sbjct: 350 ILNRMPRDNWLP*TAF*YLAKGITNSEGLALGL*NIVNWLLFAAA*AAAARTPICMLSLD 171
Query: 160 RTEEGITVCGRVACLIPSKSWFFSFHMNSTESPATSLLALTNTLQPVSCRIL 5
+T++ V A LIP +S F+ +N TESPA SL L N LQPV R L
Sbjct: 170 KTDDERLVGCLFARLIPKRSCCFASLINKTESPAISLFPLINKLQPVVSRCL 15
Score = 73.5 bits (154), Expect(2) = 8e-023
Identities = 40/90 (44%), Positives = 53/90 (58%)
Frame = -2 / -3
Query: 290 VFRKGYNKFCWTCPRIIENCEAAVXXXXXXXXCKESDVHAVTRKNRRGHHSLWPGSVSDS 111
+ K +KF T P +IE CE AV K SD+HA+TR++R S P D
Sbjct: 300 ILGKRNHKF*RTGPWVIEYCELAVICCCMSCSSKNSDMHAITRQDR**EVSRLPICSPDP 121
Query: 110 KQKLVFLIPYE*HRISSNKSLSTDKHTPAS 21
+++L+F IP +* I+SNKSLSTDK PAS
Sbjct: 120 QEELLFCIPNK*D*ITSNKSLSTDK*APAS 31
Score = 53.8 bits (111), Expect(2) = 8e-023
Identities = 24/62 (38%), Positives = 35/62 (56%)
Frame = -2 / -3
Query: 512 LNFPQRNSRWPFSCG*FIPTNLH*SPIGTRPTHWVSITHFCYCPHVPADT*FLSLK*HPE 333
LN P+ N F+ FIP NL+ +P+ P + + IT+ YC HVP+ + F LK H +
Sbjct: 522 LNVPKGNPGASFTWCCFIPCNLNIAPLRVFPPYRIQITYASYCAHVPSCSRFFGLKHHSK 343
Query: 332 SH 327
H
Sbjct: 342 PH 337
>gb|CA009554.1|CA009554 HU14H09r HU Hordeum vulgare subsp. vulgare cDNA clone HU14H09
5-PRIME, mRNA sequence
Length = 628
Score = 270 bits (584), Expect = 9e-072
Identities = 109/166 (65%), Positives = 131/166 (78%)
Frame = +3 / +1
Query: 21 TGWSVFVSAKRLVAGDSVLFIWNEKNQLLLGIRHATRPQTVMPSSVLSSDSMHIGLLXXX 200
TGWS+F+S KRL+AGDSVLFI + K QLLLGIR A R T + SSVLSSDSMHIG+L
Sbjct: 31 TGWSLFISGKRLIAGDSVLFIRDAKQQLLLGIRRANRQPTNLSSSVLSSDSMHIGVLAAA 210
Query: 201 XXXXXTNSRFTIFYNPRASPTEFVIPLSKYIKAVFHTRISVGMRFRMLFETEESSVRRYM 380
NS+FTIFYNPRASP+EFVIP +KY KAV+ ++S+GMRFRM+FETEES RRYM
Sbjct: 211 AHAAANNSQFTIFYNPRASPSEFVIPFAKYQKAVYGNQLSLGMRFRMMFETEESGTRRYM 390
Query: 381 GTITEVSDADPVRWPSSYWRSVKVGWDESTAGERPPRVSLWEIEPL 518
GTIT +SD DPVRW +S WR+++V WDE+ ER RVSLW+IEP+
Sbjct: 391 GTITGISDLDPVRWKNSQWRNIQVAWDEAAPSERRTRVSLWDIEPV 528
Score = 155 bits (332), Expect = 5e-037
Identities = 88/172 (51%), Positives = 103/172 (59%)
Frame = -3 / -3
Query: 520 VSGSISHRETLGGLSPAVDSSQPTFTDLQ*ELGQRTGSASLTSVIVPMYLRTLDSSVSNN 341
++GS+S RETL LS SS T *E TGS SL VIVPMYL DSSVSN
Sbjct: 530 MTGSMSQRETLVRLSLGAASSHAT*ILRH*EFFHLTGSRSLMPVIVPMYLLVPDSSVSNI 351
Query: 340 ILNRIPTDIRV*KTALMYFERGITNSVGLALGL*KIVKRLFVXXXXXXXARSPMCMLSLE 161
ILNR+P D + TA Y +GITNS GLALGL* IV L AR+P+CMLSL+
Sbjct: 350 ILNRMPRDNWLP*TAF*YLAKGITNSEGLALGL*NIVNWLLFAAA*AAAARTPICMLSLD 171
Query: 160 RTEEGITVCGRVACLIPSKSWFFSFHMNSTESPATSLLALTNTLQPVSCRIL 5
+T++ V A LIP +S F+ +N TESPA SL L N LQPV R L
Sbjct: 170 KTDDERLVGCLFARLIPKRSCCFASLINKTESPAISLFPLINKLQPVVSRCL 15
Score = 73.5 bits (154), Expect(2) = 8e-023
Identities = 40/90 (44%), Positives = 53/90 (58%)
Frame = -2 / -2
Query: 290 VFRKGYNKFCWTCPRIIENCEAAVXXXXXXXXCKESDVHAVTRKNRRGHHSLWPGSVSDS 111
+ K +KF T P +IE CE AV K SD+HA+TR++R S P D
Sbjct: 300 ILGKRNHKF*RTGPWVIEYCELAVICCCMSCSSKNSDMHAITRQDR**EVSRLPICSPDP 121
Query: 110 KQKLVFLIPYE*HRISSNKSLSTDKHTPAS 21
+++L+F IP +* I+SNKSLSTDK PAS
Sbjct: 120 QEELLFCIPNK*D*ITSNKSLSTDK*APAS 31
Score = 53.8 bits (111), Expect(2) = 8e-023
Identities = 24/62 (38%), Positives = 35/62 (56%)
Frame = -2 / -2
Query: 512 LNFPQRNSRWPFSCG*FIPTNLH*SPIGTRPTHWVSITHFCYCPHVPADT*FLSLK*HPE 333
LN P+ N F+ FIP NL+ +P+ P + + IT+ YC HVP+ + F LK H +
Sbjct: 522 LNVPKGNPGASFTWCCFIPCNLNIAPLRVFPPYRIQITYASYCAHVPSCSRFFGLKHHSK 343
Query: 332 SH 327
H
Sbjct: 342 PH 337
>gb|AV934271.1|AV934271 AV934271 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal11l09 5', mRNA sequence
Length = 582
Score = 113 bits (241), Expect(3) = 6e-043
Identities = 46/98 (46%), Positives = 66/98 (67%)
Frame = +3 / +3
Query: 9 IRHDTGWSVFVSAKRLVAGDSVLFIWNEKNQLLLGIRHATRPQTVMPSSVLSSDSMHIGL 188
I +GWSVFVS+KRLVAGD+ +F+ E +L +G+R A R + + SSV+SS SMH+G+
Sbjct: 39 ISFXSGWSVFVSSKRLVAGDAFIFLRGESGELRVGVRRAMRQLSNIASSVISSHSMHLGV 218
Query: 189 LXXXXXXXXTNSRFTIFYNPRASPTEFVIPLSKYIKAV 302
L T + FT++Y PR S +EF+IP KY ++V
Sbjct: 219 LATAWHAINTKTMFTVYYKPRTSRSEFIIPYDKYTESV 332
Score = 63.8 bits (133), Expect(3) = 6e-043
Identities = 24/40 (60%), Positives = 29/40 (72%)
Frame = +3 / +3
Query: 420 WPSSYWRSVKVGWDESTAGERPPRVSLWEIEPLTTFPMYP 539
WP S WRS+KV WDES+ RP RVS WEIEP ++ P+ P
Sbjct: 444 WPESSWRSLKVRWDESSTIPRPDRVSPWEIEPASSPPVNP 563
Score = 35.9 bits (72), Expect(3) = 6e-043
Identities = 16/39 (41%), Positives = 24/39 (61%)
Frame = +3 / +3
Query: 318 SVGMRFRMLFETEESSVRRYMGTITEVSDADPVRWPSSY 434
S+G RF+M FE EE+ +R+ GTI + D + SS+
Sbjct: 345 SIGTRFKMRFEGEEAPEQRFTGTIVGSDNLDQLWPESSW 461
>gb|BF621467.2|BF621467 HVSMEa0011B10f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0011B10f, mRNA sequence
Length = 815
Score = 137 bits (294), Expect(2) = 6e-034
Identities = 51/73 (69%), Positives = 64/73 (87%)
Frame = +3 / +2
Query: 306 HTRISVGMRFRMLFETEESSVRRYMGTITEVSDADPVRWPSSYWRSVKVGWDESTAGERP 485
+T++S+GMRFRMLFETE+S VRRYMGTIT + D DPVRW +S+WR+++VGWDESTA ER
Sbjct: 44 YTQVSLGMRFRMLFETEDSGVRRYMGTITGIGDLDPVRWKNSHWRNLQVGWDESTASERR 223
Query: 486 PRVSLWEIEPLTT 524
RVS+WEIEP+ T
Sbjct: 224 TRVSIWEIEPVAT 262
Score = 27.2 bits (53), Expect(2) = 6e-034
Identities = 10/14 (71%), Positives = 13/14 (92%)
Frame = +3 / +2
Query: 264 EFVIPLSKYIKAVF 305
EFVIPL+KY KA++
Sbjct: 5 EFVIPLAKYNKALY 46
>gb|CA030942.1|CA030942 HX08J07r HX Hordeum vulgare subsp. vulgare cDNA clone HX08J07
5-PRIME, mRNA sequence
Length = 622
Score = 121 bits (258), Expect(2) = 2e-032
Identities = 47/94 (50%), Positives = 68/94 (72%)
Frame = +3 / +2
Query: 21 TGWSVFVSAKRLVAGDSVLFIWNEKNQLLLGIRHATRPQTVMPSSVLSSDSMHIGLLXXX 200
+GWSVFVS+KRLVAGD+ +F+ E +L +G+R A R + +PSSV+SS SMH+G+L
Sbjct: 251 SGWSVFVSSKRLVAGDAFIFLRGESGELRVGVRRAMRQLSNVPSSVISSHSMHLGVLATA 430
Query: 201 XXXXXTNSRFTIFYNPRASPTEFVIPLSKYIKAV 302
T S FT++Y PR SP+EF+IP +Y+++V
Sbjct: 431 WHAINTKSMFTVYYKPRTSPSEFIIPYDQYMESV 532
Score = 38.2 bits (77), Expect(2) = 2e-032
Identities = 15/24 (62%), Positives = 19/24 (79%)
Frame = +3 / +2
Query: 318 SVGMRFRMLFETEESSVRRYMGTI 389
S+GMRFRM FE EE+ +R+ GTI
Sbjct: 545 SIGMRFRMRFEGEEAPEQRFTGTI 616
>gb|CA032804.1|CA032804 HX14E02r HX Hordeum vulgare subsp. vulgare cDNA clone HX14E02
5-PRIME, mRNA sequence
Length = 655
Score = 71.2 bits (149), Expect(3) = 3e-030
Identities = 26/53 (49%), Positives = 38/53 (71%)
Frame = +3 / +3
Query: 144 MPSSVLSSDSMHIGLLXXXXXXXXTNSRFTIFYNPRASPTEFVIPLSKYIKAV 302
+PSSV+SS SMH+G+L T S FT++Y PR SP+EF+IP +Y+++V
Sbjct: 57 VPSSVISSHSMHLGVLATAWHAINTKSMFTVYYKPRTSPSEFIIPYDQYMESV 215
Score = 59.7 bits (124), Expect(3) = 3e-030
Identities = 22/40 (55%), Positives = 28/40 (70%)
Frame = +3 / +3
Query: 420 WPSSYWRSVKVGWDESTAGERPPRVSLWEIEPLTTFPMYP 539
WP S WRS+KV WDE + RP RVS W+IEP ++ P+ P
Sbjct: 327 WPESNWRSLKVRWDEPSTIPRPDRVSPWKIEPASSPPVNP 446
Score = 39.1 bits (79), Expect(3) = 3e-030
Identities = 16/31 (51%), Positives = 21/31 (67%)
Frame = +3 / +3
Query: 318 SVGMRFRMLFETEESSVRRYMGTITEVSDAD 410
S+GMRFRM FE EE+ +R+ GTI + D
Sbjct: 228 SIGMRFRMRFEGEEAPEQRFTGTIVGSENLD 320
>gb|BU987774.1|BU987774 HF15L16r HF Hordeum vulgare subsp. vulgare cDNA clone HF15L16
5-PRIME, mRNA sequence
Length = 591
Score = 71.2 bits (149), Expect(3) = 3e-030
Identities = 26/53 (49%), Positives = 38/53 (71%)
Frame = +3 / +1
Query: 144 MPSSVLSSDSMHIGLLXXXXXXXXTNSRFTIFYNPRASPTEFVIPLSKYIKAV 302
+PSSV+SS SMH+G+L T S FT++Y PR SP+EF+IP +Y+++V
Sbjct: 58 VPSSVISSHSMHLGVLATAWHAINTKSMFTVYYKPRTSPSEFIIPYDQYMESV 216
Score = 59.7 bits (124), Expect(3) = 3e-030
Identities = 22/40 (55%), Positives = 28/40 (70%)
Frame = +3 / +1
Query: 420 WPSSYWRSVKVGWDESTAGERPPRVSLWEIEPLTTFPMYP 539
WP S WRS+KV WDE + RP RVS W+IEP ++ P+ P
Sbjct: 328 WPESNWRSLKVRWDEPSTIPRPDRVSPWKIEPASSPPVNP 447
Score = 39.1 bits (79), Expect(3) = 3e-030
Identities = 16/31 (51%), Positives = 21/31 (67%)
Frame = +3 / +1
Query: 318 SVGMRFRMLFETEESSVRRYMGTITEVSDAD 410
S+GMRFRM FE EE+ +R+ GTI + D
Sbjct: 229 SIGMRFRMRFEGEEAPEQRFTGTIVGSENLD 321
>gb|AV933277.1|AV933277 AV933277 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal6b18 5', mRNA sequence
Length = 667
Score = 121 bits (258), Expect = 8e-027
Identities = 47/94 (50%), Positives = 68/94 (72%)
Frame = +3 / +2
Query: 21 TGWSVFVSAKRLVAGDSVLFIWNEKNQLLLGIRHATRPQTVMPSSVLSSDSMHIGLLXXX 200
+GWSVFVS+KRLVAGD+ +F+ E +L +G+R A R + +PSSV+SS SMH+G+L
Sbjct: 386 SGWSVFVSSKRLVAGDAFIFLRGESGELRVGVRRAMRQLSNVPSSVISSHSMHLGVLATA 565
Query: 201 XXXXXTNSRFTIFYNPRASPTEFVIPLSKYIKAV 302
T S FT++Y PR SP+EF+IP +Y+++V
Sbjct: 566 WHAINTKSMFTVYYKPRTSPSEFIIPYDQYMESV 667
>gb|BJ472357.1|BJ472357 BJ472357 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal33n21 5', mRNA sequence
Length = 684
Score = 63.8 bits (133), Expect(3) = 3e-025
Identities = 24/40 (60%), Positives = 29/40 (72%)
Frame = +3 / +1
Query: 420 WPSSYWRSVKVGWDESTAGERPPRVSLWEIEPLTTFPMYP 539
WP S WRS+KV WDES+ RP RVS WEIEP ++ P+ P
Sbjct: 271 WPESSWRSLKVRWDESSTIPRPDRVSPWEIEPASSPPVNP 390
Score = 53.3 bits (110), Expect(3) = 3e-025
Identities = 19/44 (43%), Positives = 28/44 (63%)
Frame = +3 / +1
Query: 171 SMHIGLLXXXXXXXXTNSRFTIFYNPRASPTEFVIPLSKYIKAV 302
SMH+G+L T + FT++Y PR S +EF+IP KY ++V
Sbjct: 28 SMHLGVLATAWHAINTKTMFTVYYKPRTSRSEFIIPYDKYTESV 159
Score = 35.9 bits (72), Expect(3) = 3e-025
Identities = 16/39 (41%), Positives = 24/39 (61%)
Frame = +3 / +1
Query: 318 SVGMRFRMLFETEESSVRRYMGTITEVSDADPVRWPSSY 434
S+G RF+M FE EE+ +R+ GTI + D + SS+
Sbjct: 172 SIGTRFKMRFEGEEAPEQRFTGTIVGSDNLDQLWPESSW 288
>gb|CA008952.1|CA008952 HU12K18r HU Hordeum vulgare subsp. vulgare cDNA clone HU12K18
5-PRIME, mRNA sequence
Length = 609
Score = 112 bits (240), Expect = 3e-024
Identities = 45/94 (47%), Positives = 65/94 (69%)
Frame = +3 / +2
Query: 21 TGWSVFVSAKRLVAGDSVLFIWNEKNQLLLGIRHATRPQTVMPSSVLSSDSMHIGLLXXX 200
+GWSVFVS+KRLVAGD+ +F+ E +L +G+R A R + + SSV+SS SMH+G+L
Sbjct: 320 SGWSVFVSSKRLVAGDAFIFLRGESGELRVGVRRAMRQLSNIASSVISSHSMHLGVLATA 499
Query: 201 XXXXXTNSRFTIFYNPRASPTEFVIPLSKYIKAV 302
T + FT++Y PR S +EF+IP KY ++V
Sbjct: 500 WHAINTKTMFTVYYKPRTSRSEFIIPYDKYTESV 601
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 463,836,074
Number of Sequences: 312970
Number of extensions: 7196299
Number of successful extensions: 47384
Number of sequences better than 1.0e-020: 14
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 12210084310
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)