TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2950225.2.1
(3330 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV836714.1|AV836714 AV836714 K. Sato unpublished cDNA li... 170 2e-057
gb|BI951758.1|BI951758 HVSMEm0002J04f Hordeum vulgare green... 217 2e-055
gb|BJ469841.1|BJ469841 BJ469841 K. Sato unpublished cDNA li... 216 6e-055
gb|BJ473254.1|BJ473254 BJ473254 K. Sato unpublished cDNA li... 216 6e-055
gb|CA015467.1|CA015467 HT14G12r HT Hordeum vulgare subsp. v... 211 2e-053
gb|BM372123.2|BM372123 EBro03_SQ004_B12_R root, 3 week, wat... 210 4e-053
gb|CA028449.1|CA028449 HZ62A02r HZ Hordeum vulgare subsp. v... 207 4e-053
gb|AV926843.1|AV926843 AV926843 K. Sato unpublished cDNA li... 142 1e-050
gb|BI954155.1|BI954155 HVSMEm0016G19f Hordeum vulgare green... 139 2e-048
gb|BJ477369.1|BJ477369 BJ477369 K. Sato unpublished cDNA li... 115 3e-045
gb|AV923902.1|AV923902 AV923902 K. Sato unpublished cDNA li... 179 7e-045
gb|CA019492.1|CA019492 HV12C14r HV Hordeum vulgare subsp. v... 127 2e-044
gb|BJ473186.1|BJ473186 BJ473186 K. Sato unpublished cDNA li... 125 3e-040
gb|BQ766121.1|BQ766121 EBro08_SQ005_I02_R root, 3 week, dro... 154 9e-039
gb|BJ471300.1|BJ471300 BJ471300 K. Sato unpublished cDNA li... 116 1e-037
gb|CA007616.1|CA007616 HU08I04r HU Hordeum vulgare subsp. v... 120 4e-037
gb|BJ473916.1|BJ473916 BJ473916 K. Sato unpublished cDNA li... 90 2e-032
gb|BF627502.2|BF627502 HVSMEb0005A12f Hordeum vulgare seedl... 135 1e-030
gb|AV832952.1|AV832952 AV832952 K. Sato unpublished cDNA li... 90 2e-025
gb|BJ475454.1|BJ475454 BJ475454 K. Sato unpublished cDNA li... 90 8e-025
gb|BJ454304.1|BJ454304 BJ454304 K. Sato unpublished cDNA li... 112 9e-024
gb|CA015224.1|CA015224 HT13K15r HT Hordeum vulgare subsp. v... 111 2e-023
gb|BE215258.1|BE215258 HV_CEb0006E21f Hordeum vulgare seedl... 67 8e-023
emb|AJ234550.1|HVU234550 Hordeum vulgare genomic DNA fragme... 106 6e-022
>gb|AV836714.1|AV836714 AV836714 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum vulgare
subsp. vulgare cDNA clone basd26p01, mRNA sequence
Length = 635
Score = 170 bits (365), Expect(3) = 2e-057
Identities = 67/111 (60%), Positives = 94/111 (84%)
Frame = -2 / +3
Query: 2075 QKDYAQTAQMCGRALITLINDVLDRAKIEAGKLELEAVPFDLRSLMDDVVSLFSSKSREK 1896
Q+DY +TAQ G+AL++LIN+VLD+AKIE+GKLEL+AVP D+R + DD++SLF K++EK
Sbjct: 42 QQDYVRTAQASGKALVSLINEVLDQAKIESGKLELDAVPLDVRLVCDDILSLFCGKAQEK 221
Query: 1895 CIELAVFVCDNVPKVVIGDPWRFRQILTNLVGNAVKFTERGHVFVRVCLAE 1743
+ELAV+V D VP+ +IGDP R RQI+TNL+GN++KFTERGH+++ V + E
Sbjct: 222 GLELAVYVSDQVPQTLIGDPNRMRQIITNLMGNSIKFTERGHIYLTVHVVE 374
Score = 98.2 bits (208), Expect(3) = 9e-026
Identities = 54/107 (50%), Positives = 64/107 (59%)
Frame = +1 / -2
Query: 1774 PRSVNLTAFPTKFVSICRNLQGSPITTFGTLSHTNTASSMHFSRDFEENKETTSSMRERR 1953
P SVN FP +FV IC L GSPI+ GT S T TA+S FS F + ++ SS
Sbjct: 343 PLSVNFMEFPMRFVMICLILFGSPISVCGT*SETYTANSSPFS*AFPQKRDKMSSQTSLT 164
Query: 1954 SKGTASSSNFPASIFARSRTSFISVINALPHI*AV*A*SFCVIVRSV 2094
S+GTASSS+ P SIFA SRTSF+ A P AV *S V+ RSV
Sbjct: 163 SRGTASSSSLPDSIFA*SRTSFMRDTKAFPLAWAVLT*SC*VVSRSV 23
Score = 57.4 bits (119), Expect(3) = 2e-057
Identities = 22/34 (64%), Positives = 27/34 (79%)
Frame = -2 / +3
Query: 1529 DRVTLAISIEDTGVGIPLQAQDRVFTPFMQADSS 1428
+ + L IS+EDTGVGIPL+AQ R+FTPFMQ S
Sbjct: 534 ETIRLIISVEDTGVGIPLEAQSRIFTPFMQVGPS 635
Score = 36.3 bits (73), Expect(3) = 2e-057
Identities = 15/27 (55%), Positives = 17/27 (62%)
Frame = -2 / +3
Query: 1682 VESTANGAFNTLSGFEAADRRNSWQYF 1602
VE+ +NTLSG E A RR SWQ F
Sbjct: 396 VETEIQNTYNTLSGCEVAKRRLSWQSF 476
Score = 33.6 bits (67), Expect(3) = 9e-026
Identities = 13/21 (61%), Positives = 15/21 (71%)
Frame = +1 / -2
Query: 1441 CINGVKTRSCACSGIPTPVSS 1503
C+ GV R A SG+PTPVSS
Sbjct: 622 CMKGVNMRDWASSGMPTPVSS 560
Score = 24.9 bits (48), Expect(3) = 9e-026
Identities = 11/16 (68%), Positives = 11/16 (68%)
Frame = +1 / -2
Query: 1609 CQLFRLSAASNPLKVL 1656
CQL L A S PLKVL
Sbjct: 469 CQLSLLLATSQPLKVL 422
>gb|BI951758.1|BI951758 HVSMEm0002J04f Hordeum vulgare green seedling EST library HVcDNA0014
(Blumeria infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEm0002J04f, mRNA sequence
Length = 841
Score = 217 bits (469), Expect = 2e-055
Identities = 85/128 (66%), Positives = 110/128 (85%)
Frame = -2 / +2
Query: 3155 HWTLRRDSMDRAEERLVSMCEERARMLQEQFGVTVNHVHAIAILISTFNFEKSPPAIDQD 2976
H +LR +++ +AEE +V MCEERARMLQ+QF V+VNHVHA+AIL++TF++EK P A+DQD
Sbjct: 2 HRSLRHNALVKAEEGIVCMCEERARMLQDQFAVSVNHVHALAILVATFHYEKQPAALDQD 181
Query: 2975 TFAKYTARTSFERPLLNGVAFAQRVFHHEREMFESQQGWVMNTMQREPAPPQVEYAPVIF 2796
TFA YTARTSFERPLL+GVA+AQRV H +RE FE QQGW++ TM+ EP+P Q EYAP I+
Sbjct: 182 TFADYTARTSFERPLLSGVAYAQRVMHADRETFERQQGWIIKTMKHEPSPAQDEYAPGIY 361
Query: 2795 SQDTVSYL 2772
SQ+T+SY+
Sbjct: 362 SQETLSYI 385
Score = 137 bits (294), Expect = 3e-031
Identities = 68/127 (53%), Positives = 77/127 (60%)
Frame = +1 / -2
Query: 2776 *ETVS*EKITGAYSTCGGAGSRCIVFITHPCWLSNISLS*WNTRCANATPLSSGRSNDVL 2955
*+ VS E + GAYS+C G GS +V + PC S +S S TRCA ATPLSSGRS DV
Sbjct: 381 *DRVSWE*MPGAYSSCAGEGSCFMVLMIQPCCRSKVSRSACITRCAYATPLSSGRSKDVR 202
Query: 2956 AVYFAKVSWSMAGGDFSKLKVEMRMAMAWTWLTVTPNCSCSILALSSHMLTXXXXXXXXX 3135
AV AKVSWS A G FS KV RMA AWTWLT T N SCS+ A SSHM T
Sbjct: 201 AV*SAKVSWSSAAGCFS*WKVATRMARAWTWLTETANWSCSMRARSSHMHTMPSSALTSA 22
Query: 3136 XXXXVQC 3156
++C
Sbjct: 21 LWRRLRC 1
>gb|BJ469841.1|BJ469841 BJ469841 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal37n04 5', mRNA sequence
Length = 655
Score = 216 bits (466), Expect = 6e-055
Identities = 93/145 (64%), Positives = 110/145 (75%)
Frame = -2 / +2
Query: 797 SFLRSLLVGKNILVVDDNKVNLRVAAAALKKYGANVSCVESGKDAISLLQPPHRFDACFM 618
S L SLL + I+VVDDN VNL+VAA ALKKYGA V C +SGK AI+LL+PPH FDACFM
Sbjct: 35 SALGSLLHKRKIIVVDDNAVNLKVAAGALKKYGAEVICADSGKKAIALLKPPHSFDACFM 214
Query: 617 DVQMPEMDGFEATGQIRQMELKANEERKNKLASIEGSTTAEYHLPVLAMTADVIQATYEE 438
D+QMPEMDGFEAT +IR ME + NE + A E + + P+LAMTADVIQATYE
Sbjct: 215 DIQMPEMDGFEATKRIRMMERELNERVERGEAPPECANVRRWRTPILAMTADVIQATYEH 394
Query: 437 CIKSGMDGYVSKPFDEEQLYQAVSR 363
C+K MDGYVSKPF+ EQLY+ V+R
Sbjct: 395 CLKCEMDGYVSKPFEGEQLYREVTR 469
>gb|BJ473254.1|BJ473254 BJ473254 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal41f18 5', mRNA sequence
Length = 621
Score = 216 bits (466), Expect = 6e-055
Identities = 93/145 (64%), Positives = 110/145 (75%)
Frame = -2 / +3
Query: 797 SFLRSLLVGKNILVVDDNKVNLRVAAAALKKYGANVSCVESGKDAISLLQPPHRFDACFM 618
S L SLL + I+VVDDN VNL+VAA ALKKYGA V C +SGK AI+LL+PPH FDACFM
Sbjct: 15 SALGSLLHKRKIIVVDDNAVNLKVAAGALKKYGAEVICADSGKKAIALLKPPHSFDACFM 194
Query: 617 DVQMPEMDGFEATGQIRQMELKANEERKNKLASIEGSTTAEYHLPVLAMTADVIQATYEE 438
D+QMPEMDGFEAT +IR ME + NE + A E + + P+LAMTADVIQATYE
Sbjct: 195 DIQMPEMDGFEATKRIRMMERELNERVERGEAPPECANVRRWRTPILAMTADVIQATYEH 374
Query: 437 CIKSGMDGYVSKPFDEEQLYQAVSR 363
C+K MDGYVSKPF+ EQLY+ V+R
Sbjct: 375 CLKCEMDGYVSKPFEGEQLYREVTR 449
>gb|CA015467.1|CA015467 HT14G12r HT Hordeum vulgare subsp. vulgare cDNA clone HT14G12
5-PRIME, mRNA sequence
Length = 599
Score = 211 bits (455), Expect = 2e-053
Identities = 88/136 (64%), Positives = 105/136 (77%)
Frame = -2 / +3
Query: 770 KNILVVDDNKVNLRVAAAALKKYGANVSCVESGKDAISLLQPPHRFDACFMDVQMPEMDG 591
+ I+VVDDN VNL+VAA ALKKYGA V C +SGK AI+LL+PPH FDACFMD+QMPEMDG
Sbjct: 108 RKIIVVDDNAVNLKVAAGALKKYGAEVICADSGKKAIALLKPPHSFDACFMDIQMPEMDG 287
Query: 590 FEATGQIRQMELKANEERKNKLASIEGSTTAEYHLPVLAMTADVIQATYEECIKSGMDGY 411
FEAT +IR ME + NE + A E + + P+LAMTADVIQATYE C+K MDGY
Sbjct: 288 FEATKRIRMMERELNERVERGEAPPECANVRRWRTPILAMTADVIQATYEHCLKCEMDGY 467
Query: 410 VSKPFDEEQLYQAVSR 363
VSKPF+ EQLY+ V+R
Sbjct: 468 VSKPFEGEQLYREVTR 515
>gb|BM372123.2|BM372123 EBro03_SQ004_B12_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ004_B12 5', mRNA sequence
Length = 436
Score = 210 bits (453), Expect = 4e-053
Identities = 88/134 (65%), Positives = 104/134 (77%)
Frame = -2 / +3
Query: 764 ILVVDDNKVNLRVAAAALKKYGANVSCVESGKDAISLLQPPHRFDACFMDVQMPEMDGFE 585
I+VVDDN VNL+VAA ALKKYGA V C +SGK AI+LL+PPH FDACFMD+QMPEMDGFE
Sbjct: 6 IIVVDDNAVNLKVAAGALKKYGAEVICADSGKKAIALLKPPHSFDACFMDIQMPEMDGFE 185
Query: 584 ATGQIRQMELKANEERKNKLASIEGSTTAEYHLPVLAMTADVIQATYEECIKSGMDGYVS 405
AT +IR ME + NE + A E + + P+LAMTADVIQATYE C+K MDGYVS
Sbjct: 186 ATKRIRMMERELNERVERGEAPPECANVRRWRTPILAMTADVIQATYEHCLKCEMDGYVS 365
Query: 404 KPFDEEQLYQAVSR 363
KPF+ EQLY+ V+R
Sbjct: 366 KPFEGEQLYREVTR 407
>gb|CA028449.1|CA028449 HZ62A02r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ62A02
5-PRIME, mRNA sequence
Length = 629
Score = 207 bits (446), Expect(2) = 4e-053
Identities = 92/151 (60%), Positives = 109/151 (72%)
Frame = -2 / +3
Query: 836 VEIAERKDNQNRPSFLRSLLVGKNILVVDDNKVNLRVAAAALKKYGANVSCVESGKDAIS 657
V+ A K+ S L SLL + I+VVDDN VNL+VAA ALKKYGA V C +SGK AI+
Sbjct: 177 VDKAHVKNGLVGNSALGSLLHKRKIIVVDDNAVNLKVAAGALKKYGAEVICADSGKKAIA 356
Query: 656 LLQPPHRFDACFMDVQMPEMDGFEATGQIRQMELKANEERKNKLASIEGSTTAEYHLPVL 477
LL+PPH FDACFMD+QMPEMDGFEAT +IR ME + NE + A E + + P+L
Sbjct: 357 LLKPPHSFDACFMDIQMPEMDGFEATKRIRMMERELNERVERGEAPPECANVRRWRTPIL 536
Query: 476 AMTADVIQATYEECIKSGMDGYVSKPFDEEQ 384
AMTADVIQATYE C+K MDGYVSKPF+ EQ
Sbjct: 537 AMTADVIQATYEHCLKCEMDGYVSKPFEGEQ 629
Score = 23.5 bits (45), Expect(2) = 4e-053
Identities = 8/19 (42%), Positives = 16/19 (84%)
Frame = -2 / +3
Query: 896 VMCKPIRASTIASSIQQLL 840
V+ KP+RAS + +S++++L
Sbjct: 114 VIMKPLRASMLEASLRRVL 170
>gb|AV926843.1|AV926843 AV926843 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd23p17 5', mRNA sequence
Length = 609
Score = 142 bits (305), Expect(3) = 1e-050
Identities = 63/98 (64%), Positives = 74/98 (75%)
Frame = -2 / +2
Query: 836 VEIAERKDNQNRPSFLRSLLVGKNILVVDDNKVNLRVAAAALKKYGANVSCVESGKDAIS 657
V+ A K+ S L SLL + I+VVDDN VNL+VAA ALKKYGA V C +SGK AI+
Sbjct: 149 VDKAHVKNGLVGNSALGSLLHKRKIIVVDDNAVNLKVAAGALKKYGAEVICADSGKKAIA 328
Query: 656 LLQPPHRFDACFMDVQMPEMDGFEATGQIRQMELKANE 543
LL+PPH FDACFMD+QMPEMDGFEAT +IR ME + NE
Sbjct: 329 LLKPPHSFDACFMDIQMPEMDGFEATKRIRMMERELNE 442
Score = 74.8 bits (157), Expect(3) = 1e-050
Identities = 29/39 (74%), Positives = 33/39 (84%)
Frame = -2 / +3
Query: 494 YHLPVLAMTADVIQATYEECIKSGMDGYVSKPFDEEQLY 378
+ P+LAMTADVIQATYE C+K MDGYVSKPF+ EQLY
Sbjct: 492 WRTPILAMTADVIQATYEHCLKCEMDGYVSKPFEGEQLY 608
Score = 23.5 bits (45), Expect(3) = 1e-050
Identities = 8/19 (42%), Positives = 16/19 (84%)
Frame = -2 / +2
Query: 896 VMCKPIRASTIASSIQQLL 840
V+ KP+RAS + +S++++L
Sbjct: 86 VIMKPLRASMLEASLRRVL 142
>gb|BI954155.1|BI954155 HVSMEm0016G19f Hordeum vulgare green seedling EST library HVcDNA0014
(Blumeria infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEm0016G19f, mRNA sequence
Length = 648
Score = 139 bits (299), Expect(2) = 2e-048
Identities = 54/102 (52%), Positives = 77/102 (75%)
Frame = -2 / +1
Query: 3134 SMDRAEERLVSMCEERARMLQEQFGVTVNHVHAIAILISTFNFEKSPPAIDQDTFAKYTA 2955
++++ + L SMC+ERARMLQ+QF V++NH+ A+AIL++TF+ ++P AI Q TFA+Y
Sbjct: 73 AVEKRRDSLASMCDERARMLQDQFNVSMNHLQALAILVNTFHHSQTPSAITQATFARYAE 252
Query: 2954 RTSFERPLLNGVAFAQRVFHHEREMFESQQGWVMNTMQREPA 2829
RT+FERPL +GVA+A RV H ER+ FE QQGW + M P+
Sbjct: 253 RTAFERPLTSGVAYAVRVTHAERDQFERQQGWSIKKMYSSPS 378
Score = 95.5 bits (202), Expect(2) = 7e-027
Identities = 51/123 (41%), Positives = 60/123 (48%)
Frame = +1 / -2
Query: 2833 GSRCIVFITHPCWLSNISLS*WNTRCANATPLSSGRSNDVLAVYFAKVSWSMAGGDFSKL 3012
G I + HPC SN S S TR A ATPL SGRSN VL+ Y A V+W MA G
Sbjct: 374 GEEYIFLMLHPCCRSNWSRSACVTRTAYATPLVSGRSNAVLSAYLANVAWVMAEGVCEWW 195
Query: 3013 KVEMRMAMAWTWLTVTPNCSCSILALSSHMLTXXXXXXXXXXXXXVQCITAEQTTAVSTH 3192
KV RMA AW W +T N S S+ A SSHML + + + +TH
Sbjct: 194 KVLTRMASAWRWFMLTLNWSWSMRARSSHMLASESRRFSTAPELEYRKTCRQMSVQPTTH 15
Query: 3193 PAA 3201
A
Sbjct: 14 SIA 6
Score = 75.3 bits (158), Expect(2) = 2e-048
Identities = 33/64 (51%), Positives = 41/64 (64%)
Frame = -2 / +1
Query: 2825 PQVEYAPVIFSQDTVSYLARIDMMSGEEDRENIFRARTTGKAVLTNPFRLLGSNHLGVVL 2646
P EYAPVIF+QD + DM+SG +DRENI RAR +GK VLT PF+LL ++
Sbjct: 421 PADEYAPVIFAQDAYKNVISFDMLSGADDRENIIRARESGKGVLTAPFQLLNGRLRVILT 600
Query: 2645 TFAV 2634
F V
Sbjct: 601 YFRV 612
Score = 47.3 bits (97), Expect(2) = 7e-027
Identities = 25/51 (49%), Positives = 30/51 (58%)
Frame = +1 / -2
Query: 2674 SNRNGFVNTALPVVLARKMFSRSSSPDIMSMRAR*ETVS*EKITGAYSTCG 2826
S+ G V T P LAR MFSRSS+P+ MS S K+TGAYS+ G
Sbjct: 572 SSWKGAVRTPFPDSLARMMFSRSSAPESMSKEMTFL*ASCAKMTGAYSSAG 420
>gb|BJ477369.1|BJ477369 BJ477369 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal38p18 3', mRNA sequence
Length = 731
Score = 115 bits (245), Expect(2) = 3e-045
Identities = 46/62 (74%), Positives = 51/62 (82%)
Frame = -2 / -2
Query: 731 RVAAAALKKYGANVSCVESGKDAISLLQPPHRFDACFMDVQMPEMDGFEATGQIRQMELK 552
RVAA LKKYGA V CVESGKDA+ LLQ PH+FD C MD+QMPEMDGFEAT QIR +E K
Sbjct: 730 RVAAGTLKKYGAKVXCVESGKDALELLQAPHKFDLCLMDIQMPEMDGFEATRQIRAIEAK 551
Query: 551 AN 546
A+
Sbjct: 550 AH 545
Score = 89.5 bits (189), Expect(2) = 3e-045
Identities = 34/46 (73%), Positives = 43/46 (93%)
Frame = -2 / -2
Query: 494 YHLPVLAMTADVIQATYEECIKSGMDGYVSKPFDEEQLYQAVSRLV 357
+HLPVLAMTADVIQAT+EEC K+GMDGYVSKPF+E+QL++AV + +
Sbjct: 499 WHLPVLAMTADVIQATHEECTKNGMDGYVSKPFEEKQLFKAVQKFL 362
Score = 75.3 bits (158), Expect(2) = 7e-027
Identities = 29/59 (49%), Positives = 41/59 (69%)
Frame = +3 / +2
Query: 555 QLHLPYLSGCLKPVHLWHLNIHKTCIEAMRGLK*TDSILAAFNTANISTIFLECSRSNS 731
+ + PYL GCLKP+HLWHLNIH T I+ + GL+ SI + + ++ IFL+C+ SNS
Sbjct: 554 RFNCPYLPGCLKPIHLWHLNIH*TEIKLVWGLQKLKSIFSTLHALHLCPIFLQCTSSNS 730
Score = 67.5 bits (141), Expect(2) = 7e-027
Identities = 30/43 (69%), Positives = 34/43 (79%)
Frame = +1 / +3
Query: 361 NLETAWYSCSSSKGLDTYPSIPDFMHSS*VAWITSAVIARTGR 489
N TA +C SSKG +T PSIP F+HSS VAW+TSAVIARTGR
Sbjct: 366 NFCTALNNCFSSKGFET*PSIPFFVHSSWVAWMTSAVIARTGR 494
>gb|AV923902.1|AV923902 AV923902 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd20a22 5', mRNA sequence
Length = 566
Score = 179 bits (386), Expect(2) = 7e-045
Identities = 82/140 (58%), Positives = 98/140 (70%)
Frame = -2 / +2
Query: 836 VEIAERKDNQNRPSFLRSLLVGKNILVVDDNKVNLRVAAAALKKYGANVSCVESGKDAIS 657
V+ A K+ S L SLL + I+VVDDN VNL+VAA ALKKYGA V C +SGK AI+
Sbjct: 146 VDKAHVKNGLVGNSALGSLLHKRKIIVVDDNAVNLKVAAGALKKYGAEVICADSGKKAIA 325
Query: 656 LLQPPHRFDACFMDVQMPEMDGFEATGQIRQMELKANEERKNKLASIEGSTTAEYHLPVL 477
LL+PPH FDACFMD+QMPEMDGFEAT +IR ME + NE + A E + + P+L
Sbjct: 326 LLKPPHSFDACFMDIQMPEMDGFEATKRIRMMERELNERVERGEAPPECANVRRWRTPIL 505
Query: 476 AMTADVIQATYEECIKSGMD 417
AMTADVIQATYE +K MD
Sbjct: 506 AMTADVIQATYEHXLKCEMD 565
Score = 23.5 bits (45), Expect(2) = 7e-045
Identities = 8/19 (42%), Positives = 16/19 (84%)
Frame = -2 / +2
Query: 896 VMCKPIRASTIASSIQQLL 840
V+ KP+RAS + +S++++L
Sbjct: 83 VIMKPLRASMLEASLRRVL 139
>gb|CA019492.1|CA019492 HV12C14r HV Hordeum vulgare subsp. vulgare cDNA clone HV12C14
5-PRIME, mRNA sequence
Length = 478
Score = 127 bits (271), Expect(2) = 2e-044
Identities = 50/89 (56%), Positives = 68/89 (76%)
Frame = -2 / +1
Query: 3095 EERARMLQEQFGVTVNHVHAIAILISTFNFEKSPPAIDQDTFAKYTARTSFERPLLNGVA 2916
+ERARMLQ+QF V++NH+ A+AIL++TF+ ++P AI Q TFA+Y RT+FERPL +GVA
Sbjct: 13 DERARMLQDQFNVSMNHLQALAILVNTFHHSQTPSAITQATFARYAERTAFERPLTSGVA 192
Query: 2915 FAQRVFHHEREMFESQQGWVMNTMQREPA 2829
+A RV H ER+ FE QQGW + M P+
Sbjct: 193 YAVRVTHAERDQFERQQGWSIKKMYSSPS 279
Score = 87.2 bits (184), Expect(2) = 2e-024
Identities = 45/88 (51%), Positives = 50/88 (56%)
Frame = +1 / -3
Query: 2833 GSRCIVFITHPCWLSNISLS*WNTRCANATPLSSGRSNDVLAVYFAKVSWSMAGGDFSKL 3012
G I + HPC SN S S TR A ATPL SGRSN VL+ Y A V+W MA G
Sbjct: 275 GEEYIFLMLHPCCRSNWSRSACVTRTAYATPLVSGRSNAVLSAYLANVAWVMAEGVCEWW 96
Query: 3013 KVEMRMAMAWTWLTVTPNCSCSILALSS 3096
KV RMA AW W +T N S S+ A SS
Sbjct: 95 KVLTRMASAWRWFMLTLNWSWSMRARSS 12
Score = 74.4 bits (156), Expect(2) = 2e-044
Identities = 31/51 (60%), Positives = 37/51 (72%)
Frame = -2 / +1
Query: 2825 PQVEYAPVIFSQDTVSYLARIDMMSGEEDRENIFRARTTGKAVLTNPFRLL 2673
P EYAPVIF+QD + DM+SG +DRENI RAR +GK VLT PF+LL
Sbjct: 322 PADEYAPVIFAQDAYKNVISFDMLSGADDRENIIRARESGKGVLTAPFQLL 474
Score = 47.3 bits (97), Expect(2) = 2e-024
Identities = 25/51 (49%), Positives = 30/51 (58%)
Frame = +1 / -3
Query: 2674 SNRNGFVNTALPVVLARKMFSRSSSPDIMSMRAR*ETVS*EKITGAYSTCG 2826
S+ G V T P LAR MFSRSS+P+ MS S K+TGAYS+ G
Sbjct: 473 SSWKGAVRTPFPDSLARMMFSRSSAPESMSKEMTFL*ASCAKMTGAYSSAG 321
>gb|BJ473186.1|BJ473186 BJ473186 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal38p18 5', mRNA sequence
Length = 721
Score = 125 bits (268), Expect(2) = 3e-040
Identities = 51/80 (63%), Positives = 60/80 (75%)
Frame = -2 / +1
Query: 842 LKVEIAERKDNQNRPSFLRSLLVGKNILVVDDNKVNLRVAAAALKKYGANVSCVESGKDA 663
+ + A + + P L +LL+GKNILVVDDNKVNLRVAA LKKYGA V CVESGKDA
Sbjct: 481 ISITQANNEKKHHGPDSLYALLLGKNILVVDDNKVNLRVAAGTLKKYGAKVECVESGKDA 660
Query: 662 ISLLQPPHRFDACFMDVQMP 603
+ LLQ PH+FD C MD+QMP
Sbjct: 661 LELLQAPHKFDLCLMDIQMP 720
Score = 66.6 bits (139), Expect(2) = 3e-040
Identities = 28/75 (37%), Positives = 44/75 (58%)
Frame = -2 / +1
Query: 1304 ATLKHSYKDISGDSSRSLTEALPTAFKGMKAILVDGRPVRSAVTRYHLKRLGILLQVVNN 1125
A L+ ++ S +L LP++F+G+ +LVD RPVR+ VT YHL+RLGI + V
Sbjct: 1 AVLQRCERNAINVSKSALLHPLPSSFQGLSTLLVDKRPVRATVTEYHLQRLGIASKSVRT 180
Query: 1124 MNAVVKAFPGQNGAA 1080
+ + G+NG +
Sbjct: 181 IEQALSVLLGRNGTS 225
>gb|BQ766121.1|BQ766121 EBro08_SQ005_I02_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ005_I02 5', mRNA sequence
Length = 685
Score = 154 bits (330), Expect(2) = 9e-039
Identities = 64/99 (64%), Positives = 75/99 (75%)
Frame = -2 / +3
Query: 842 LKVEIAERKDNQNRPSFLRSLLVGKNILVVDDNKVNLRVAAAALKKYGANVSCVESGKDA 663
+ + A + + P L +LL+GKNILVVDDNKVNLRVAA LKKYGA V CVESGKDA
Sbjct: 357 ISITQANNEKKHHGPDSLYALLLGKNILVVDDNKVNLRVAAGTLKKYGAKVECVESGKDA 536
Query: 662 ISLLQPPHRFDACFMDVQMPEMDGFEATGQIRQMELKAN 546
+ LLQ PH+FD C MD+QMPEMDGFEAT IR +E KA+
Sbjct: 537 LELLQAPHKFDLCLMDIQMPEMDGFEATRXIRAIEAKAH 653
Score = 33.1 bits (66), Expect(2) = 9e-039
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = -2 / +3
Query: 1178 VTRYHLKRLGILLQVVNNMNAVVKAFPGQNGAA 1080
VT YHL+RLGI + V + + G+NG +
Sbjct: 3 VTEYHLQRLGIASKSVRTIEQALSVLLGRNGTS 101
>gb|BJ471300.1|BJ471300 BJ471300 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal17l08 5', mRNA sequence
Length = 723
Score = 116 bits (248), Expect(2) = 1e-037
Identities = 48/76 (63%), Positives = 56/76 (73%)
Frame = -2 / +1
Query: 842 LKVEIAERKDNQNRPSFLRSLLVGKNILVVDDNKVNLRVAAAALKKYGANVSCVESGKDA 663
+ + A + + P L +LL+GKNILVVDDNKVNLRVAA LKKYGA V CVESGKDA
Sbjct: 496 ISITQANNEKKHHGPDSLYALLLGKNILVVDDNKVNLRVAAGTLKKYGAKVECVESGKDA 675
Query: 662 ISLLQPPHRFDACFMD 615
+ LLQ PH+FD C MD
Sbjct: 676 LELLQAPHKFDLCLMD 723
Score = 67.0 bits (140), Expect(2) = 1e-037
Identities = 28/76 (36%), Positives = 45/76 (59%)
Frame = -2 / +1
Query: 1307 SATLKHSYKDISGDSSRSLTEALPTAFKGMKAILVDGRPVRSAVTRYHLKRLGILLQVVN 1128
+A L+ ++ S +L LP++F+G+ +LVD RPVR+ VT YHL+RLGI + V
Sbjct: 13 AAVLQRCERNAINVSKSALLHPLPSSFQGLSTLLVDKRPVRATVTEYHLQRLGIASKSVR 192
Query: 1127 NMNAVVKAFPGQNGAA 1080
+ + G+NG +
Sbjct: 193 TIEQALSVLLGRNGTS 240
>gb|CA007616.1|CA007616 HU08I04r HU Hordeum vulgare subsp. vulgare cDNA clone HU08I04
5-PRIME, mRNA sequence
Length = 622
Score = 120 bits (257), Expect(2) = 4e-037
Identities = 50/81 (61%), Positives = 61/81 (75%)
Frame = -2 / +1
Query: 1529 DRVTLAISIEDTGVGIPLQAQDRVFTPFMQADSSTSRNYGGTGIGLSISKCLAELMGGQI 1350
+ + L IS+EDTGVGIPL+AQ R+FTPFMQ S +R +GGTGIGLSISKCL LM G+I
Sbjct: 67 ETIRLIISVEDTGVGIPLEAQSRIFTPFMQVGPSIARIHGGTGIGLSISKCLVHLMKGEI 246
Query: 1349 SFTSHPSVGSTFTFSATLKHS 1287
F S VGSTF+F+A L +
Sbjct: 247 GFVSKHHVGSTFSFTAVLSRA 309
Score = 56.5 bits (117), Expect(2) = 4e-037
Identities = 20/40 (50%), Positives = 33/40 (82%)
Frame = -2 / +1
Query: 1229 FKGMKAILVDGRPVRSAVTRYHLKRLGILLQVVNNMNAVV 1110
FKG+ A++VD RPVR+ VT+YHL+RLG+ ++ N+++ V+
Sbjct: 343 FKGVSALVVDHRPVRANVTKYHLQRLGVKTELTNDVSQVI 462
>gb|BJ473916.1|BJ473916 BJ473916 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal17l08 3', mRNA sequence
Length = 650
Score = 89.5 bits (189), Expect(2) = 2e-032
Identities = 34/46 (73%), Positives = 43/46 (93%)
Frame = -2 / -2
Query: 494 YHLPVLAMTADVIQATYEECIKSGMDGYVSKPFDEEQLYQAVSRLV 357
+HLPVLAMTADVIQAT+EEC K+GMDGYVSKPF+E+QL++AV + +
Sbjct: 487 WHLPVLAMTADVIQATHEECTKNGMDGYVSKPFEEKQLFKAVQKFL 350
Score = 71.6 bits (150), Expect(2) = 2e-032
Identities = 27/39 (69%), Positives = 32/39 (82%)
Frame = -2 / -2
Query: 662 ISLLQPPHRFDACFMDVQMPEMDGFEATGQIRQMELKAN 546
+ LLQ PH+FD C MD+QMPEMDGFEAT QIR +E KA+
Sbjct: 649 LELLQAPHKFDLCLMDIQMPEMDGFEATRQIRAIEAKAH 533
Score = 67.5 bits (141), Expect(2) = 5e-021
Identities = 30/43 (69%), Positives = 34/43 (79%)
Frame = +1 / +3
Query: 361 NLETAWYSCSSSKGLDTYPSIPDFMHSS*VAWITSAVIARTGR 489
N TA +C SSKG +T PSIP F+HSS VAW+TSAVIARTGR
Sbjct: 354 NFCTALNNCFSSKGFET*PSIPFFVHSSWVAWMTSAVIARTGR 482
Score = 55.6 bits (115), Expect(2) = 5e-021
Identities = 20/33 (60%), Positives = 26/33 (78%)
Frame = +3 / +2
Query: 555 QLHLPYLSGCLKPVHLWHLNIHKTCIEAMRGLK 653
+ + PYL GCLKP+HLWHLNIH T I+ + GL+
Sbjct: 542 RFNCPYLPGCLKPIHLWHLNIH*TEIKLVWGLQ 640
>gb|BF627502.2|BF627502 HVSMEb0005A12f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0005A12f, mRNA sequence
Length = 773
Score = 135 bits (290), Expect = 1e-030
Identities = 55/84 (65%), Positives = 64/84 (76%)
Frame = -2 / +3
Query: 842 LKVEIAERKDNQNRPSFLRSLLVGKNILVVDDNKVNLRVAAAALKKYGANVSCVESGKDA 663
+ + A + + P L +LL+GKNILVVDDNKVNLRVAA LKKYGA V CVESGKDA
Sbjct: 231 ISITQANNEKKHHGPDSLYALLLGKNILVVDDNKVNLRVAAGTLKKYGAKVECVESGKDA 410
Query: 662 ISLLQPPHRFDACFMDVQMPEMDG 591
+ LLQ PH+FD C MD+QMPEMDG
Sbjct: 411 LELLQAPHKFDLCLMDIQMPEMDG 482
>gb|AV832952.1|AV832952 AV832952 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare top three leaves adult, heading stage Hordeum
vulgare subsp. vulgare cDNA clone baal4h11, mRNA
sequence
Length = 622
Score = 89.5 bits (189), Expect(2) = 2e-025
Identities = 34/46 (73%), Positives = 43/46 (93%)
Frame = -2 / -3
Query: 494 YHLPVLAMTADVIQATYEECIKSGMDGYVSKPFDEEQLYQAVSRLV 357
+HLPVLAMTADVIQAT+EEC K+GMDGYVSKPF+E+QL++AV + +
Sbjct: 500 WHLPVLAMTADVIQATHEECTKNGMDGYVSKPFEEKQLFKAVQKFL 363
Score = 48.3 bits (99), Expect(2) = 2e-025
Identities = 19/25 (76%), Positives = 22/25 (88%)
Frame = -2 / -3
Query: 620 MDVQMPEMDGFEATGQIRQMELKAN 546
MD+QMPEMDGFEAT QIR +E KA+
Sbjct: 620 MDIQMPEMDGFEATRQIRAIEAKAH 546
>gb|BJ475454.1|BJ475454 BJ475454 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal25l05 3', mRNA sequence
Length = 606
Score = 89.5 bits (189), Expect(2) = 8e-025
Identities = 34/46 (73%), Positives = 43/46 (93%)
Frame = -2 / -2
Query: 494 YHLPVLAMTADVIQATYEECIKSGMDGYVSKPFDEEQLYQAVSRLV 357
+HLPVLAMTADVIQAT+EEC K+GMDGYVSKPF+E+QL++AV + +
Sbjct: 485 WHLPVLAMTADVIQATHEECTKNGMDGYVSKPFEEKQLFKAVQKFL 348
Score = 46.4 bits (95), Expect(2) = 8e-025
Identities = 18/25 (72%), Positives = 21/25 (84%)
Frame = -2 / -2
Query: 620 MDVQMPEMDGFEATGQIRQMELKAN 546
MD+QMPEMDGFE T QIR +E KA+
Sbjct: 605 MDIQMPEMDGFEXTRQIRAIEAKAH 531
>gb|BJ454304.1|BJ454304 BJ454304 K. Sato unpublished cDNA library, cv. Akashinriki vegetative
stage leaves Hordeum vulgare subsp. vulgare cDNA clone
baak46o20 5', mRNA sequence
Length = 648
Score = 112 bits (240), Expect = 9e-024
Identities = 54/160 (33%), Positives = 83/160 (51%)
Frame = -2 / +2
Query: 2246 DKVTEDCRKMEELKTQAEAADVAKSQFLATASHEIRTPMNGVXXXXXXXXXXXXXXTQKD 2067
D + E ++ + AE A A++ FLA +HE+RTPMN + Q+
Sbjct: 131 DLLMEQNVALDLARRDAETAIRARNDFLAVMNHEMRTPMNAIIALSSLLLETELTPEQRL 310
Query: 2066 YAQTAQMCGRALITLINDVLDRAKIEAGKLELEAVPFDLRSLMDDVVSLFSSKSREKCIE 1887
+T L TLINDVLD +K+E LELE F+L ++ +V+ + K +
Sbjct: 311 MVETVLKSSNLLATLINDVLDLSKLEDRSLELEIRAFNLHAVFKEVMGFIRPIAAIKRLS 490
Query: 1886 LAVFVCDNVPKVVIGDPWRFRQILTNLVGNAVKFTERGHV 1767
++V + ++P IGD R Q + N+ GNAVKFT+ GH+
Sbjct: 491 MSVMLAPDLPLCAIGDEKRLMQTILNISGNAVKFTKEGHI 610
>gb|CA015224.1|CA015224 HT13K15r HT Hordeum vulgare subsp. vulgare cDNA clone HT13K15
5-PRIME, mRNA sequence
Length = 647
Score = 111 bits (238), Expect = 2e-023
Identities = 53/160 (33%), Positives = 83/160 (51%)
Frame = -2 / +1
Query: 2246 DKVTEDCRKMEELKTQAEAADVAKSQFLATASHEIRTPMNGVXXXXXXXXXXXXXXTQKD 2067
D + E ++ + AE A A++ FLA +HE+RTPMN + Q+
Sbjct: 55 DLLMEQNVALDLARRDAETAIRARNDFLAVMNHEMRTPMNAIIALSSLLLETELTPEQRL 234
Query: 2066 YAQTAQMCGRALITLINDVLDRAKIEAGKLELEAVPFDLRSLMDDVVSLFSSKSREKCIE 1887
+T L TLINDVLD +K+E G EL+ F+L ++ +V+S + K +
Sbjct: 235 MVETVLKSSNLLATLINDVLDLSKLEDGSFELDISAFNLHAVFKEVMSFVKPIAAIKKLS 414
Query: 1886 LAVFVCDNVPKVVIGDPWRFRQILTNLVGNAVKFTERGHV 1767
++ + ++P IGD R Q + N+ GNAVKFT+ GH+
Sbjct: 415 VSAMLSPDLPLSAIGDEKRLMQTVLNVCGNAVKFTKEGHI 534
>gb|BE215258.1|BE215258 HV_CEb0006E21f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0006E21f, mRNA sequence
Length = 677
Score = 66.6 bits (139), Expect(4) = 8e-023
Identities = 32/58 (55%), Positives = 40/58 (68%)
Frame = -2 / +1
Query: 836 VEIAERKDNQNRPSFLRSLLVGKNILVVDDNKVNLRVAAAALKKYGANVSCVESGKDA 663
V+ A K+ S L SLL + I+VVDDN VNL+VAA ALKKYGA V C +SGK++
Sbjct: 451 VDKAHVKNGLVGNSALGSLLHKRKIIVVDDNAVNLKVAAGALKKYGAEVICADSGKES 624
Score = 56.5 bits (117), Expect(4) = 8e-023
Identities = 20/40 (50%), Positives = 33/40 (82%)
Frame = -2 / +1
Query: 1229 FKGMKAILVDGRPVRSAVTRYHLKRLGILLQVVNNMNAVV 1110
FKG+ A++VD RPVR+ VT+YHL+RLG+ ++ N+++ V+
Sbjct: 64 FKGVSALVVDHRPVRANVTKYHLQRLGVKTELTNDVSQVI 183
Score = 27.2 bits (53), Expect(4) = 8e-023
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = -2 / +2
Query: 674 GKDAISLLQPPHRF 633
GK AI+LL PPH F
Sbjct: 614 GKKAIALLPPPHSF 655
Score = 23.5 bits (45), Expect(4) = 8e-023
Identities = 8/19 (42%), Positives = 16/19 (84%)
Frame = -2 / +1
Query: 896 VMCKPIRASTIASSIQQLL 840
V+ KP+RAS + +S++++L
Sbjct: 388 VIMKPLRASMLEASLRRVL 444
>emb|AJ234550.1|HVU234550 Hordeum vulgare genomic DNA fragment; clone MWG0635.uni
Length = 255
Score = 106 bits (227), Expect = 6e-022
Identities = 41/62 (66%), Positives = 52/62 (83%)
Frame = -2 / -1
Query: 2930 LNGVAFAQRVFHHEREMFESQQGWVMNTMQREPAPPQVEYAPVIFSQDTVSYLARIDMMS 2751
L+GVA+AQRV H +RE FE QQGW++ TM+ EP+P Q EYAPVI+SQ+TVSY+ +DMMS
Sbjct: 255 LSGVAYAQRVMHADRETFERQQGWIIKTMKHEPSPAQDEYAPVIYSQETVSYIEGLDMMS 76
Query: 2750 GE 2745
GE
Sbjct: 75 GE 70
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 1,557,322,405
Number of Sequences: 312970
Number of extensions: 25240996
Number of successful extensions: 134223
Number of sequences better than 1.0e-020: 33
length of database: 58,378,179
effective HSP length: 56
effective length of database: 40,851,859
effective search space used: 43017007527
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)