TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2950080.2.1
(687 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AL504465.1|AL504465 AL504465 Hordeum vulgare Barke roots... 346 1e-094
gb|BI957056.1|BI957056 HVSMEn0007D01f Hordeum vulgare rachi... 149 4e-050
>gb|AL504465.1|AL504465 AL504465 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW05F23V 5', mRNA sequence
Length = 579
Score = 346 bits (749), Expect = 1e-094
Identities = 136/168 (80%), Positives = 146/168 (86%)
Frame = +2 / +3
Query: 38 ARPNVLVXXXXXXXXXXXCSLLADAAGLRHVNIGDLVREKSLHDGWDADLECHVINEDLV 217
ARPNVL+ CSLLADAAG+ HVNIGDLVREK LHDGWD DL+CHVINEDLV
Sbjct: 69 ARPNVLMTGTPGTGKTTTCSLLADAAGVAHVNIGDLVREKGLHDGWDEDLDCHVINEDLV 248
Query: 218 CDELEDMMEEGGILVDYHGCDFFPERWFDLVVVLQTDNSILHDRLTGRGYMGSKLANNIE 397
CDELED MEEGG+LVDYHGCDFFPERWFDLV LQTDNSILHDRLT RGYMG+KL NNIE
Sbjct: 249 CDELEDRMEEGGVLVDYHGCDFFPERWFDLVXXLQTDNSILHDRLTSRGYMGAKLTNNIE 428
Query: 398 CEIFQVLLEEARESYREDIVMPLRSDNVEDISRNVGALTDWVNNWRPS 541
CEIFQVLLEEARESY+EDIVMPLRSD+V DIS+NV LT+W+NNWRPS
Sbjct: 429 CEIFQVLLEEARESYKEDIVMPLRSDSVXDISKNVSTLTEWINNWRPS 572
Score = 187 bits (403), Expect(2) = 2e-062
Identities = 81/118 (68%), Positives = 95/118 (80%)
Frame = -3 / -2
Query: 535 SPIVYPVCQCTYVPTNILHVVTSQWHHDVLSVALPRLLEEHLEDFALYVVGELGTHVTSA 356
SP+VYP+CQ +V +IL VTSQ HH +L VALPRLL EHLED LYVV ELGTHV SA
Sbjct: 566 SPVVYPLCQRAHVLADILDAVTSQRHHYILLVALPRLL*EHLEDLTLYVVCELGTHVASA 387
Query: 355 RQAIMQN*VVSLEHNYEVKPTFWKEITSVVIY*YTTFFHHVLKLVAHQVLVDDMALEV 182
QAIMQN*VV L+ ++VKPT WKEITSV+IY YT FFH VL+L+ +QVLVDD+A+EV
Sbjct: 386 SQAIMQN*VVGLKXXHKVKPTLWKEITSVIIYQYTAFFHPVLELITNQVLVDDVAVEV 213
Score = 156 bits (336), Expect = 9e-038
Identities = 78/113 (69%), Positives = 83/113 (73%)
Frame = +1 / +2
Query: 199 HQRGPGVRRA*GHDGRRWYTSRLPRM*FLSRTLV*PRSCAPD*QLNSA*SPDGQRLHGFQ 378
HQRGPG+ A G DGRR T RL RM*FLSR LV*P A D QLNSA*S D QRLHG Q
Sbjct: 230 HQRGPGL**ARGQDGRRRCTGRLSRM*FLSRALV*PCGRASDRQLNSA*SLD*QRLHGCQ 409
Query: 379 ARQQHRVRNLPSAPRGGEGELQRGHRDAIAK*QRGGY**ERRCIDRLGKQLET 537
A +QHRV +LP APRGGEGELQ G+ DA AK*QR GY ER DR+ KQLET
Sbjct: 410 AHKQHRV*DLPGAPRGGEGELQGGYSDAAAK*QRXGYQQEREHADRVDKQLET 568
Score = 142 bits (304), Expect(2) = 4e-034
Identities = 62/104 (59%), Positives = 71/104 (68%)
Frame = -2 / -1
Query: 536 VSNCLPSLSMHLRSY*YPPRCHFAMAXXXXXXXXXXXXRGALGRFRTLCCWRAWNPCNLC 357
VS+CL +LS RS *YP RCHFA A GA GR TLCC AW+PC+LC
Sbjct: 567 VSSCLSTLSACSRSC*YPXRCHFAAASLYPPCSSPSPPLGAPGRSHTLCCL*AWHPCSLC 388
Query: 356 PSGDHAELSCQSGAQLRGQTNVLERNHIRGNLLVYHLLPSCPQA 225
S DHAELSC+S A+ +GQTN LERNHIR NL V+ LLPSCP+A
Sbjct: 387 *SSDHAELSCRSEARPQGQTNALERNHIRDNLPVHRLLPSCPRA 256
Score = 113 bits (242), Expect = 8e-025
Identities = 54/79 (68%), Positives = 58/79 (73%)
Frame = -1 / -3
Query: 516 SVNAPTFLLISSTLSLRNGITMXXXXXXXXXXXSTWKISHSMLLASLEPM*PLPVRRSCR 337
SV+ TFLLIS TLSLR+GIT+ STWKISHSML SL PM*PL V+RSCR
Sbjct: 547 SVSVLTFLLISXTLSLRSGITISSL*LSLASSRSTWKISHSMLFVSLAPM*PLLVKRSCR 368
Query: 336 IELSVWSTTTRSNQRSGKK 280
IELSV S TRSNQRSGKK
Sbjct: 367 IELSV*SXXTRSNQRSGKK 311
Score = 95.0 bits (201), Expect(2) = 1e-024
Identities = 48/92 (52%), Positives = 50/92 (54%)
Frame = +3 / +1
Query: 99 SLPTPQXXXXXXXXXXXXXXXXXXXXXPTSSAMSSTRTWCATSLRT*WKKVVY**ITTDV 278
S PT TS+A SSTRTW S RT WKK VY *I TDV
Sbjct: 130 SSPTRPAWPTSTSATSSARRGSTTAGTRTSTATSSTRTWFVMSSRTGWKKAVYW*IITDV 309
Query: 279 ISFQNVGLTS*LCSRLTTQFCMIA*RAEVTWV 374
ISFQ+VGLT R TTQFCMIA* AE TWV
Sbjct: 310 ISFQSVGLTLWXXFRPTTQFCMIA*LAEATWV 405
Score = 71.6 bits (150), Expect(2) = 2e-062
Identities = 35/56 (62%), Positives = 36/56 (64%)
Frame = -1 / -3
Query: 204 LMTWHSRSASQPS*RLFSRTRSPMLTWRRPAASARSEHXXXXXXXXXXVTSTFGRA 37
LMTW SRS+SQPS FSRTRSPMLTW PAASAR E V ST GRA
Sbjct: 235 LMTWQSRSSSQPSWSPFSRTRSPMLTWATPAASARREXVVVFPVPGVPVISTLGRA 68
Score = 37.7 bits (76), Expect(2) = 1e-024
Identities = 19/32 (59%), Positives = 20/32 (62%)
Frame = +3 / +1
Query: 441 TERTS*CHCEVTTWRILVGT*VH*QTG*TIGD 536
T R *C CEVT RI T* *Q+G*T GD
Sbjct: 472 TRRI**CRCEVTASRISART*AR*QSG*TTGD 567
Score = 22.6 bits (43), Expect(2) = 4e-034
Identities = 8/19 (42%), Positives = 14/19 (73%)
Frame = -1 / -3
Query: 153 SRTRSPMLTWRRPAASARS 97
S+TRS ++TW+ ++S S
Sbjct: 253 SQTRSSLMTWQSRSSSQPS 197
>gb|BI957056.1|BI957056 HVSMEn0007D01f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0007D01f, mRNA sequence
Length = 626
Score = 149 bits (320), Expect(2) = 4e-050
Identities = 59/71 (83%), Positives = 66/71 (92%)
Frame = +2 / -2
Query: 329 NSILHDRLTGRGYMGSKLANNIECEIFQVLLEEARESYREDIVMPLRSDNVEDISRNVGA 508
NSILHDRLT RGYMG+KL NNIECEIFQVLLE+ARESY+EDIVM LRSD+VEDIS+NV
Sbjct: 541 NSILHDRLTSRGYMGAKLTNNIECEIFQVLLEKARESYKEDIVMLLRSDSVEDISKNVST 362
Query: 509 LTDWVNNWRPS 541
LT+W+NNWRPS
Sbjct: 361 LTEWINNWRPS 329
Score = 111 bits (238), Expect(2) = 4e-033
Identities = 50/70 (71%), Positives = 55/70 (78%)
Frame = -3 / +2
Query: 535 SPIVYPVCQCTYVPTNILHVVTSQWHHDVLSVALPRLLEEHLEDFALYVVGELGTHVTSA 356
SP+VYP+CQ +V +IL VTSQ HH +L VALPRLL EHLED LYVV ELGTHV SA
Sbjct: 335 SPVVYPLCQRAHVLADILDAVTSQQHHYILLVALPRLL*EHLEDLTLYVVCELGTHVASA 514
Query: 355 RQAIMQN*VV 326
QAIMQN*VV
Sbjct: 515 SQAIMQN*VV 544
Score = 99.1 bits (210), Expect(2) = 1e-025
Identities = 48/70 (68%), Positives = 52/70 (74%)
Frame = +1 / -1
Query: 328 QLNSA*SPDGQRLHGFQARQQHRVRNLPSAPRGGEGELQRGHRDAIAK*QRGGY**ERRC 507
QLN A*S D QRLHG QA +QHRV +LP APR GEGELQ G+ DA AK*QR GY ER
Sbjct: 542 QLNFA*SLD*QRLHGCQAHKQHRV*DLPGAPREGEGELQGGYSDAAAK*QRRGYQQEREH 363
Query: 508 IDRLGKQLET 537
DR+ KQLET
Sbjct: 362 ADRVDKQLET 333
Score = 87.7 bits (185), Expect(2) = 2e-023
Identities = 38/70 (54%), Positives = 43/70 (61%)
Frame = -2 / +1
Query: 536 VSNCLPSLSMHLRSY*YPPRCHFAMAXXXXXXXXXXXXRGALGRFRTLCCWRAWNPCNLC 357
VS+CL +LS RS *YP RCHFA A GA GR TLCC AW+PC+LC
Sbjct: 334 VSSCLSTLSACSRSC*YPRRCHFAAASLYPPCSSPSPSLGAPGRSHTLCCL*AWHPCSLC 513
Query: 356 PSGDHAELSC 327
S DHA+LSC
Sbjct: 514 *SSDHAKLSC 543
Score = 68.9 bits (144), Expect(2) = 4e-050
Identities = 25/27 (92%), Positives = 26/27 (96%)
Frame = +2 / -3
Query: 245 EGGILVDYHGCDFFPERWFDLVVVLQT 325
EGG+LVDYHGCDF PERWFDLVVVLQT
Sbjct: 624 EGGVLVDYHGCDFSPERWFDLVVVLQT 544
Score = 49.2 bits (101), Expect(2) = 4e-033
Identities = 17/26 (65%), Positives = 23/26 (88%)
Frame = -3 / +1
Query: 322 LEHNYEVKPTFWKEITSVVIY*YTTF 245
L+H+++VKPT W+EITSV+IY YT F
Sbjct: 547 LKHDHKVKPTLWREITSVIIYQYTAF 624
Score = 40.9 bits (83), Expect(2) = 2e-023
Identities = 17/27 (62%), Positives = 21/27 (77%)
Frame = -2 / +3
Query: 323 SGAQLRGQTNVLERNHIRGNLLVYHLL 243
S A+ +GQTN LERNHIR NL ++ LL
Sbjct: 546 SEARPQGQTNALERNHIRDNLPIHRLL 626
Score = 36.8 bits (74), Expect(2) = 1e-025
Identities = 19/32 (59%), Positives = 20/32 (62%)
Frame = +3 / -1
Query: 243 KKVVY**ITTDVISFQNVGLTS*LCSRLTTQF 338
KK VY *I TDVIS Q+VGLT C R F
Sbjct: 626 KKAVYW*IITDVISLQSVGLTLWSCFRPQLNF 531
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 269,943,248
Number of Sequences: 312970
Number of extensions: 3876662
Number of successful extensions: 45896
Number of sequences better than 1.0e-020: 4
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7507757493
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)