TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2921718.2.1
(649 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CV059284.1|CV059284 BNEL46d8 Barley EST endosperm librar... 271 3e-072
gb|CA031834.1|CA031834 HX11E06r HX Hordeum vulgare subsp. v... 256 7e-068
gb|AV925892.1|AV925892 AV925892 K. Sato unpublished cDNA li... 252 2e-066
gb|AV928662.1|AV928662 AV928662 K. Sato unpublished cDNA li... 252 2e-066
gb|AV935887.1|AV935887 AV935887 K. Sato unpublished cDNA li... 252 2e-066
gb|CV055547.1|CV055547 BNEL120h12 Barley EST endosperm libr... 223 1e-058
gb|BU976140.1|BU976140 HA03D09u HA Hordeum vulgare subsp. v... 223 8e-058
gb|CV062825.1|CV062825 BNEL83e1 Barley EST endosperm librar... 223 8e-058
gb|CV062563.1|CV062563 BNEL80d8 Barley EST endosperm librar... 151 5e-042
gb|BU972274.1|BU972274 HB21C22r BC Hordeum vulgare subsp. v... 169 2e-041
gb|BQ657408.1|BQ657408 HA08H22u HA Hordeum vulgare subsp. v... 160 9e-039
gb|CV059060.1|CV059060 BNEL43h6 Barley EST endosperm librar... 153 6e-037
gb|BI777049.2|BI777049 EBpi03_SQ001_M01_R pistil, 4 DPA, no... 88 7e-032
gb|CV056920.1|CV056920 BNEL22a9 Barley EST endosperm librar... 136 7e-032
gb|BU985075.1|BU985075 HF06A23r HF Hordeum vulgare subsp. v... 136 1e-031
gb|BQ760241.1|BQ760241 EBpi07_SQ003_K16_R pistil, 12 DPA, n... 78 2e-027
gb|CV055258.1|CV055258 BNEL118f1 Barley EST endosperm libra... 119 2e-026
>gb|CV059284.1|CV059284 BNEL46d8 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL46d8 5' similar to COG1208, GCD1,
Nucleoside-diphosphate-sugar pyrophosphorylase involved
in lipopolysaccharide biosynthesis/translation
initiation factor 2B, gamma/epsilon subunits, mRNA
sequence
Length = 637
Score = 271 bits (586), Expect = 3e-072
Identities = 111/123 (90%), Positives = 116/123 (94%)
Frame = +2 / +2
Query: 5 RHEARLASGAHVLGNVLVHETAVIGDGCLIGPDVAVGPGCVVEAGVRLSRCTVMRAARVK 184
R ARLASGAHVL NVLVHETA IG+GCLIGPDVAVGP CVVEAGVRLSRCTVMR ARVK
Sbjct: 257 RSPARLASGAHVLXNVLVHETATIGEGCLIGPDVAVGPRCVVEAGVRLSRCTVMRGARVK 436
Query: 185 QHACVSSSIIGWHSTVGKWARVENMTILGEDVHVCDEIYSNGGVVLPHKEIKSSILKPEI 364
+HAC+S SI+GWHSTVGKWARVENMTILGEDVHVCDE+YSNGGVVLPHKEIKSSILKPEI
Sbjct: 437 KHACISGSIVGWHSTVGKWARVENMTILGEDVHVCDEVYSNGGVVLPHKEIKSSILKPEI 616
Query: 365 VM* 373
VM*
Sbjct: 617 VM* 625
Score = 221 bits (478), Expect = 2e-057
Identities = 97/120 (80%), Positives = 101/120 (84%)
Frame = -2 / -2
Query: 372 HMTISGLRMLDLISLCGSTTPPLL*ISSQTWTSSPRMVMFSTRAHLPTVECQPMMLLETQ 193
HMTISGLRMLDLISLCGSTTPPLL SSQT TSSPRMVMFSTRAHLPTVEC P ML E
Sbjct: 624 HMTISGLRMLDLISLCGSTTPPLLYTSSQTCTSSPRMVMFSTRAHLPTVECHPTMLPEMH 445
Query: 192 ACCLTRAARMTVQRDRRTPASTTQPGPTATSGPMRQPSPMTAVSCTSTLPRTCAPDASLA 13
AC TRA R+TV D RTPASTTQ GPTATSGP+R PSP+ AVS TSTL RTCAP+ASLA
Sbjct: 444 ACFFTRAPRITVHLDSRTPASTTQRGPTATSGPIRHPSPIVAVSWTSTLXRTCAPEASLA 265
Score = 68.9 bits (144), Expect(2) = 8e-022
Identities = 30/50 (60%), Positives = 32/50 (64%)
Frame = -1 / -1
Query: 166 HDGAAGQAHPGLHHAAWPDRDVGPDEAAVPDDRGLVHQHVAQDVRARREP 17
H GA Q H GLHHA D V PD+A PD R LV QHVAQDVRA +P
Sbjct: 418 HHGAPRQPHXGLHHATGADGHVRPDQAPFPDRRRLVDQHVAQDVRAGGQP 269
Score = 54.2 bits (112), Expect(2) = 8e-022
Identities = 25/44 (56%), Positives = 25/44 (56%)
Frame = -1 / -1
Query: 373 SHDDLGLEDAGFDLLVREHXXXXXXXXXXXXXXXXXDGHVLDAR 242
SHDDLGLEDAG DLLVREH DGHVL AR
Sbjct: 625 SHDDLGLEDAGLDLLVREHDAAVAVHLVADVHVLAEDGHVLHAR 494
>gb|CA031834.1|CA031834 HX11E06r HX Hordeum vulgare subsp. vulgare cDNA clone HX11E06
5-PRIME, mRNA sequence
Length = 620
Score = 256 bits (554), Expect = 7e-068
Identities = 102/112 (91%), Positives = 107/112 (95%)
Frame = +2 / +3
Query: 5 RHEARLASGAHVLGNVLVHETAVIGDGCLIGPDVAVGPGCVVEAGVRLSRCTVMRAARVK 184
R ARLASGAHVLGNVLVHETA IG+GCLIGPDVAVGPGCVVEAGVRLSRCTVMR ARVK
Sbjct: 285 RSPARLASGAHVLGNVLVHETATIGEGCLIGPDVAVGPGCVVEAGVRLSRCTVMRGARVK 464
Query: 185 QHACVSSSIIGWHSTVGKWARVENMTILGEDVHVCDEIYSNGGVVLPHKEIK 340
+HAC+S SI+GWHSTVGKWARVENMTILGEDVHVCDE+YSNGGVVLPHKEIK
Sbjct: 465 KHACISGSIVGWHSTVGKWARVENMTILGEDVHVCDEVYSNGGVVLPHKEIK 620
Score = 202 bits (435), Expect = 2e-051
Identities = 87/109 (79%), Positives = 91/109 (83%)
Frame = -2 / -2
Query: 339 LISLCGSTTPPLL*ISSQTWTSSPRMVMFSTRAHLPTVECQPMMLLETQACCLTRAARMT 160
LISLCGSTTPPLL SSQT TSSPRMVMFSTRAHLPTVEC P ML E AC TRA R+T
Sbjct: 619 LISLCGSTTPPLLYTSSQTCTSSPRMVMFSTRAHLPTVECHPTMLPEMHACFFTRAPRIT 440
Query: 159 VQRDRRTPASTTQPGPTATSGPMRQPSPMTAVSCTSTLPRTCAPDASLA 13
V D RTPASTT PGPTATSGP+R PSP+ AVS TSTLPRTCAP+ASLA
Sbjct: 439 VHLDSRTPASTTHPGPTATSGPIRHPSPIVAVSWTSTLPRTCAPEASLA 293
>gb|AV925892.1|AV925892 AV925892 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basdd19 5', mRNA sequence
Length = 612
Score = 252 bits (544), Expect = 2e-066
Identities = 100/121 (82%), Positives = 113/121 (93%)
Frame = +2 / +1
Query: 14 ARLASGAHVLGNVLVHETAVIGDGCLIGPDVAVGPGCVVEAGVRLSRCTVMRAARVKQHA 193
A+LA G H +GNVLVHE+A IG+GCLIGPDVA+GPGCVVE GVRLSRCTVMR R+K+HA
Sbjct: 217 AKLAVGEHFVGNVLVHESAKIGEGCLIGPDVAIGPGCVVEDGVRLSRCTVMRGVRIKKHA 396
Query: 194 CVSSSIIGWHSTVGKWARVENMTILGEDVHVCDEIYSNGGVVLPHKEIKSSILKPEIVM* 373
C+S+SIIGWHSTVG+WAR+ENMTILGEDVHV DE+YSNGGVVLPHKEIKSSILKPEIVM*
Sbjct: 397 CISNSIIGWHSTVGQWARIENMTILGEDVHVGDEVYSNGGVVLPHKEIKSSILKPEIVM* 576
Query: 374 S 376
+
Sbjct: 577 A 579
Score = 207 bits (447), Expect = 4e-053
Identities = 87/121 (71%), Positives = 97/121 (80%)
Frame = -2 / -2
Query: 372 HMTISGLRMLDLISLCGSTTPPLL*ISSQTWTSSPRMVMFSTRAHLPTVECQPMMLLETQ 193
HMTISGLRMLDLISLCG TTPPLL* SS T TSSPR+V+FS RAH PTVECQPMML E
Sbjct: 575 HMTISGLRMLDLISLCGRTTPPLL*TSSPTCTSSPRIVIFSMRAHCPTVECQPMMLFEMH 396
Query: 192 ACCLTRAARMTVQRDRRTPASTTQPGPTATSGPMRQPSPMTAVSCTSTLPRTCAPDASLA 13
AC L R R+TV R+ TP+STT PGP ATSGP+RQPSP+ A+SCT T P C+P ASLA
Sbjct: 395 ACFLMRTPRITVHRESLTPSSTTHPGPIATSGPIRQPSPILALSCTRTFPTKCSPTASLA 216
Query: 12 S 10
+
Sbjct: 215 A 213
>gb|AV928662.1|AV928662 AV928662 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd15d19 3', mRNA sequence
Length = 673
Score = 252 bits (544), Expect = 2e-066
Identities = 100/121 (82%), Positives = 113/121 (93%)
Frame = +2 / -1
Query: 14 ARLASGAHVLGNVLVHETAVIGDGCLIGPDVAVGPGCVVEAGVRLSRCTVMRAARVKQHA 193
A+LA G H +GNVLVHE+A IG+GCLIGPDVA+GPGCVVE GVRLSRCTVMR R+K+HA
Sbjct: 466 AKLAVGEHFVGNVLVHESAKIGEGCLIGPDVAIGPGCVVEDGVRLSRCTVMRGVRIKKHA 287
Query: 194 CVSSSIIGWHSTVGKWARVENMTILGEDVHVCDEIYSNGGVVLPHKEIKSSILKPEIVM* 373
C+S+SIIGWHSTVG+WAR+ENMTILGEDVHV DE+YSNGGVVLPHKEIKSSILKPEIVM*
Sbjct: 286 CISNSIIGWHSTVGQWARIENMTILGEDVHVGDEVYSNGGVVLPHKEIKSSILKPEIVM* 107
Query: 374 S 376
+
Sbjct: 106 A 104
Score = 207 bits (447), Expect = 4e-053
Identities = 87/121 (71%), Positives = 97/121 (80%)
Frame = -2 / +3
Query: 372 HMTISGLRMLDLISLCGSTTPPLL*ISSQTWTSSPRMVMFSTRAHLPTVECQPMMLLETQ 193
HMTISGLRMLDLISLCG TTPPLL* SS T TSSPR+V+FS RAH PTVECQPMML E
Sbjct: 108 HMTISGLRMLDLISLCGRTTPPLL*TSSPTCTSSPRIVIFSMRAHCPTVECQPMMLFEMH 287
Query: 192 ACCLTRAARMTVQRDRRTPASTTQPGPTATSGPMRQPSPMTAVSCTSTLPRTCAPDASLA 13
AC L R R+TV R+ TP+STT PGP ATSGP+RQPSP+ A+SCT T P C+P ASLA
Sbjct: 288 ACFLMRTPRITVHRESLTPSSTTHPGPIATSGPIRQPSPILALSCTRTFPTKCSPTASLA 467
Query: 12 S 10
+
Sbjct: 468 A 470
>gb|AV935887.1|AV935887 AV935887 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal6n22 3', mRNA sequence
Length = 651
Score = 252 bits (544), Expect = 2e-066
Identities = 100/121 (82%), Positives = 113/121 (93%)
Frame = +2 / -2
Query: 14 ARLASGAHVLGNVLVHETAVIGDGCLIGPDVAVGPGCVVEAGVRLSRCTVMRAARVKQHA 193
A+LA G H +GNVLVHE+A IG+GCLIGPDVA+GPGCVVE GVRLSRCTVMR R+K+HA
Sbjct: 434 AKLAVGEHFVGNVLVHESAKIGEGCLIGPDVAIGPGCVVEDGVRLSRCTVMRGVRIKKHA 255
Query: 194 CVSSSIIGWHSTVGKWARVENMTILGEDVHVCDEIYSNGGVVLPHKEIKSSILKPEIVM* 373
C+S+SIIGWHSTVG+WAR+ENMTILGEDVHV DE+YSNGGVVLPHKEIKSSILKPEIVM*
Sbjct: 254 CISNSIIGWHSTVGQWARIENMTILGEDVHVGDEVYSNGGVVLPHKEIKSSILKPEIVM* 75
Query: 374 S 376
+
Sbjct: 74 A 72
Score = 207 bits (447), Expect = 4e-053
Identities = 87/121 (71%), Positives = 97/121 (80%)
Frame = -2 / +1
Query: 372 HMTISGLRMLDLISLCGSTTPPLL*ISSQTWTSSPRMVMFSTRAHLPTVECQPMMLLETQ 193
HMTISGLRMLDLISLCG TTPPLL* SS T TSSPR+V+FS RAH PTVECQPMML E
Sbjct: 76 HMTISGLRMLDLISLCGRTTPPLL*TSSPTCTSSPRIVIFSMRAHCPTVECQPMMLFEMH 255
Query: 192 ACCLTRAARMTVQRDRRTPASTTQPGPTATSGPMRQPSPMTAVSCTSTLPRTCAPDASLA 13
AC L R R+TV R+ TP+STT PGP ATSGP+RQPSP+ A+SCT T P C+P ASLA
Sbjct: 256 ACFLMRTPRITVHRESLTPSSTTHPGPIATSGPIRQPSPILALSCTRTFPTKCSPTASLA 435
Query: 12 S 10
+
Sbjct: 436 A 438
>gb|CV055547.1|CV055547 BNEL120h12 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL120h12 5' similar to GDP-mannose
pyrophosphorylase, mRNA sequence
Length = 730
Score = 223 bits (482), Expect(3) = 1e-058
Identities = 89/99 (89%), Positives = 94/99 (94%)
Frame = +2 / -3
Query: 77 GDGCLIGPDVAVGPGCVVEAGVRLSRCTVMRAARVKQHACVSSSIIGWHSTVGKWARVEN 256
G GCLIGP VAVGPGCVV+ GVRLSRCTVMR ARVK+HAC+S SI+GWHSTVGKWARVEN
Sbjct: 605 GXGCLIGPXVAVGPGCVVKPGVRLSRCTVMRGARVKKHACISGSIVGWHSTVGKWARVEN 426
Query: 257 MTILGEDVHVCDEIYSNGGVVLPHKEIKSSILKPEIVM* 373
MTILGEDVHVCDE+YSNGGVVLPHKEIKSSILKPEIVM*
Sbjct: 425 MTILGEDVHVCDEVYSNGGVVLPHKEIKSSILKPEIVM* 309
Score = 183 bits (395), Expect = 6e-046
Identities = 78/99 (78%), Positives = 80/99 (80%)
Frame = -2 / +1
Query: 372 HMTISGLRMLDLISLCGSTTPPLL*ISSQTWTSSPRMVMFSTRAHLPTVECQPMMLLETQ 193
HMTISGLRMLDLISLCGSTTPPLL SSQT TSSPRMVMFSTRAHLPTVEC P ML E
Sbjct: 310 HMTISGLRMLDLISLCGSTTPPLLYTSSQTCTSSPRMVMFSTRAHLPTVECHPTMLPEMH 489
Query: 192 ACCLTRAARMTVQRDRRTPASTTQPGPTATSGPMRQPSP 76
AC TRA R+TV DRRTP TT PGPTAT GP+R P P
Sbjct: 490 ACFFTRAPRITVHLDRRTPGFTTHPGPTATXGPIRHPXP 606
Score = 22.6 bits (43), Expect(3) = 1e-058
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = +3 / -2
Query: 30 ARTSWATCW 56
ARTSWA W
Sbjct: 654 ARTSWANVW 628
Score = 21.7 bits (41), Expect(3) = 1e-058
Identities = 8/15 (53%), Positives = 11/15 (73%)
Frame = +3 / -1
Query: 603 LSDEWK*QLLFV*KK 647
+SDEW+* + F KK
Sbjct: 58 VSDEWE*SISFTTKK 14
>gb|BU976140.1|BU976140 HA03D09u HA Hordeum vulgare subsp. vulgare cDNA clone HA03D09
3-PRIME, mRNA sequence
Length = 485
Score = 223 bits (481), Expect = 8e-058
Identities = 88/103 (85%), Positives = 98/103 (95%)
Frame = +2 / -1
Query: 68 AVIGDGCLIGPDVAVGPGCVVEAGVRLSRCTVMRAARVKQHACVSSSIIGWHSTVGKWAR 247
A IG+GCLIGPDVA+GPGCVVE GVRLSRCTVMR R+K+HAC+S+SIIGWHSTVG+WAR
Sbjct: 485 AKIGEGCLIGPDVAIGPGCVVEDGVRLSRCTVMRGVRIKKHACISNSIIGWHSTVGQWAR 306
Query: 248 VENMTILGEDVHVCDEIYSNGGVVLPHKEIKSSILKPEIVM*S 376
+ENMTILGEDVHV DE+YSNGGVVLPHKEIKSSILKPEIVM*+
Sbjct: 305 IENMTILGEDVHVGDEVYSNGGVVLPHKEIKSSILKPEIVM*A 177
Score = 178 bits (383), Expect = 3e-044
Identities = 75/100 (75%), Positives = 82/100 (82%)
Frame = -2 / +1
Query: 372 HMTISGLRMLDLISLCGSTTPPLL*ISSQTWTSSPRMVMFSTRAHLPTVECQPMMLLETQ 193
HMTISGLRMLDLISLCG TTPPLL* SS T TSSPR+V+FS RAH PTVECQPMML E
Sbjct: 181 HMTISGLRMLDLISLCGRTTPPLL*TSSPTCTSSPRIVIFSMRAHCPTVECQPMMLFEMH 360
Query: 192 ACCLTRAARMTVQRDRRTPASTTQPGPTATSGPMRQPSPM 73
AC L R R+TV R+ TP+STT PGP ATSGP+RQPSP+
Sbjct: 361 ACFLMRTPRITVHRESLTPSSTTHPGPIATSGPIRQPSPI 480
>gb|CV062825.1|CV062825 BNEL83e1 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL83e1 5' similar to GDP-mannose
pyrophosphorylase, mRNA sequence
Length = 595
Score = 223 bits (481), Expect = 8e-058
Identities = 89/98 (90%), Positives = 94/98 (95%)
Frame = +2 / -3
Query: 80 DGCLIGPDVAVGPGCVVEAGVRLSRCTVMRAARVKQHACVSSSIIGWHSTVGKWARVENM 259
+GCLIGPDVAVG GCVV AGVRLSRCTVMR ARVK+HAC+S SI+GWHSTVGKWARVENM
Sbjct: 593 EGCLIGPDVAVGTGCVVXAGVRLSRCTVMRGARVKKHACISGSIVGWHSTVGKWARVENM 414
Query: 260 TILGEDVHVCDEIYSNGGVVLPHKEIKSSILKPEIVM* 373
TILGEDVHVCDE+YSNGGVVLPHKEIKSSILKPEIVM*
Sbjct: 413 TILGEDVHVCDEVYSNGGVVLPHKEIKSSILKPEIVM* 300
Score = 180 bits (387), Expect = 8e-045
Identities = 78/98 (79%), Positives = 80/98 (81%)
Frame = -2 / +1
Query: 372 HMTISGLRMLDLISLCGSTTPPLL*ISSQTWTSSPRMVMFSTRAHLPTVECQPMMLLETQ 193
HMTISGLRMLDLISLCGSTTPPLL SSQT TSSPRMVMFSTRAHLPTVEC P ML E
Sbjct: 301 HMTISGLRMLDLISLCGSTTPPLLYTSSQTCTSSPRMVMFSTRAHLPTVECHPTMLPEMH 480
Query: 192 ACCLTRAARMTVQRDRRTPASTTQPGPTATSGPMRQPS 79
AC TRA R+TV D RTPA TT P PTATSGP+R PS
Sbjct: 481 ACFFTRAPRITVHLDSRTPAXTTHPVPTATSGPIRHPS 594
>gb|CV062563.1|CV062563 BNEL80d8 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL80d8 5' similar to COG1208, GCD1,
Nucleoside-diphosphate-sugar pyrophosphorylase involved
in lipopolysaccharide biosynthesis/translation
initiation factor 2B, gamma/epsilon subunits, mRNA
sequence
Length = 702
Score = 151 bits (324), Expect(2) = 5e-042
Identities = 62/77 (80%), Positives = 66/77 (85%)
Frame = +2 / +3
Query: 5 RHEARLASGAHVLGNVLVHETAVIGDGCLIGPDVAVGPGCVVEAGVRLSRCTVMRAARVK 184
R ARLASGAHVL NVLVHETA IG+GCLIGPDVAVGPGCVV+A VRL RCTVMR ARVK
Sbjct: 372 RSPARLASGAHVLXNVLVHETATIGEGCLIGPDVAVGPGCVVDARVRLVRCTVMRGARVK 551
Query: 185 QHACVSSSIIGWHSTVG 235
HAC+S SI GWHS +G
Sbjct: 552 MHACISGSIGGWHSRLG 602
Score = 118 bits (253), Expect(2) = 6e-030
Identities = 51/75 (68%), Positives = 56/75 (74%)
Frame = -2 / -3
Query: 237 LPTVECQPMMLLETQACCLTRAARMTVQRDRRTPASTTQPGPTATSGPMRQPSPMTAVSC 58
LP +EC P ML E AC TRA R+TV RT ASTT PGPTATSGP+R PSP+ AVS
Sbjct: 604 LPNLECHPPMLPEMHACIFTRAPRITVHLTSRTRASTTHPGPTATSGPIRHPSPIVAVSW 425
Query: 57 TSTLPRTCAPDASLA 13
TSTL RTCAP+ASLA
Sbjct: 424 TSTLXRTCAPEASLA 380
Score = 39.6 bits (80), Expect(2) = 5e-042
Identities = 16/26 (61%), Positives = 18/26 (69%)
Frame = +2 / +1
Query: 254 NMTILGEDVHVCDEIYSNGGVVLPHK 331
+MTILG VHV +Y NG VLPHK
Sbjct: 619 DMTILGVGVHVFXRVYKNGRRVLPHK 696
Score = 31.8 bits (63), Expect(2) = 6e-030
Identities = 14/25 (56%), Positives = 16/25 (64%)
Frame = -2 / -2
Query: 330 LCGSTTPPLL*ISSQTWTSSPRMVM 256
LCGST P L +T T +PRMVM
Sbjct: 695 LCGSTRRPFLYTRXKT*TPTPRMVM 621
>gb|BU972274.1|BU972274 HB21C22r BC Hordeum vulgare subsp. vulgare cDNA clone HB21C22
5-PRIME, mRNA sequence
Length = 433
Score = 169 bits (363), Expect = 2e-041
Identities = 68/78 (87%), Positives = 72/78 (92%)
Frame = +2 / +1
Query: 5 RHEARLASGAHVLGNVLVHETAVIGDGCLIGPDVAVGPGCVVEAGVRLSRCTVMRAARVK 184
R ARLASGAHVLGNVLVHETA IG+GCLIGPDVAVGPGCVVEAGVRLSRCTVMR ARVK
Sbjct: 199 RSPARLASGAHVLGNVLVHETATIGEGCLIGPDVAVGPGCVVEAGVRLSRCTVMRGARVK 378
Query: 185 QHACVSSSIIGWHSTVGK 238
+HAC+S SI+ WHSTVGK
Sbjct: 379 KHACISGSIVXWHSTVGK 432
Score = 129 bits (277), Expect = 1e-029
Identities = 55/75 (73%), Positives = 59/75 (78%)
Frame = -2 / -3
Query: 237 LPTVECQPMMLLETQACCLTRAARMTVQRDRRTPASTTQPGPTATSGPMRQPSPMTAVSC 58
LPTVEC ML E AC TRA R+TV D RTPASTT PGPTATSGP+R PSP+ AVS
Sbjct: 431 LPTVECHXTMLPEMHACFFTRAPRITVHLDSRTPASTTHPGPTATSGPIRHPSPIVAVSW 252
Query: 57 TSTLPRTCAPDASLA 13
TSTLPRTCAP+ASLA
Sbjct: 251 TSTLPRTCAPEASLA 207
>gb|BQ657408.1|BQ657408 HA08H22u HA Hordeum vulgare subsp. vulgare cDNA clone HA08H22
3-PRIME, mRNA sequence
Length = 497
Score = 160 bits (343), Expect = 9e-039
Identities = 63/69 (91%), Positives = 67/69 (97%)
Frame = +2 / -2
Query: 167 RAARVKQHACVSSSIIGWHSTVGKWARVENMTILGEDVHVCDEIYSNGGVVLPHKEIKSS 346
R ARVK+HAC+S SI+GWHSTVGKWARVENMTILGEDVHVCDE+YSNGGVVLPHKEIKSS
Sbjct: 496 RGARVKKHACISGSIVGWHSTVGKWARVENMTILGEDVHVCDEVYSNGGVVLPHKEIKSS 317
Query: 347 ILKPEIVM* 373
ILKPEIVM*
Sbjct: 316 ILKPEIVM* 290
Score = 133 bits (284), Expect = 1e-030
Identities = 58/69 (84%), Positives = 58/69 (84%)
Frame = -2 / +3
Query: 372 HMTISGLRMLDLISLCGSTTPPLL*ISSQTWTSSPRMVMFSTRAHLPTVECQPMMLLETQ 193
HMTISGLRMLDLISLCGSTTPPLL SSQT TSSPRMVMFSTRAHLPTVEC P ML E
Sbjct: 291 HMTISGLRMLDLISLCGSTTPPLLYTSSQTCTSSPRMVMFSTRAHLPTVECHPTMLPEMH 470
Query: 192 ACCLTRAAR 166
AC TRA R
Sbjct: 471 ACFFTRAPR 497
>gb|CV059060.1|CV059060 BNEL43h6 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL43h6 5' similar to GDP-mannose
pyrophosphorylase, mRNA sequence
Length = 500
Score = 153 bits (329), Expect(2) = 6e-037
Identities = 60/65 (92%), Positives = 64/65 (98%)
Frame = +2 / -3
Query: 179 VKQHACVSSSIIGWHSTVGKWARVENMTILGEDVHVCDEIYSNGGVVLPHKEIKSSILKP 358
VK+HAC+S SI+GWHSTVGKWARVENMTILGEDVHVCDE+YSNGGVVLPHKEIKSSILKP
Sbjct: 498 VKKHACISGSIVGWHSTVGKWARVENMTILGEDVHVCDEVYSNGGVVLPHKEIKSSILKP 319
Query: 359 EIVM* 373
EIVM*
Sbjct: 318 EIVM* 304
Score = 127 bits (271), Expect(2) = 1e-029
Identities = 55/65 (84%), Positives = 55/65 (84%)
Frame = -2 / +2
Query: 372 HMTISGLRMLDLISLCGSTTPPLL*ISSQTWTSSPRMVMFSTRAHLPTVECQPMMLLETQ 193
HMTISGLRMLDLISLCGSTTPPLL SSQT TSSPRMVMFSTRAHLPTVEC P ML E
Sbjct: 305 HMTISGLRMLDLISLCGSTTPPLLYTSSQTCTSSPRMVMFSTRAHLPTVECHPTMLPEMH 484
Query: 192 ACCLT 178
AC T
Sbjct: 485 ACFFT 499
Score = 24.0 bits (46), Expect(2) = 1e-029
Identities = 10/18 (55%), Positives = 10/18 (55%)
Frame = -1 / +3
Query: 649 FFFYTKRSCYFHSSDRRL 596
FFF K Y HSSD L
Sbjct: 6 FFFLVKLMLYSHSSDTYL 59
Score = 21.7 bits (41), Expect(2) = 6e-037
Identities = 8/15 (53%), Positives = 11/15 (73%)
Frame = +3 / -2
Query: 603 LSDEWK*QLLFV*KK 647
+SDEW+* + F KK
Sbjct: 52 VSDEWE*SISFTKKK 8
>gb|BI777049.2|BI777049 EBpi03_SQ001_M01_R pistil, 4 DPA, no treatment, cv Optic, EBpi03
Hordeum vulgare subsp. vulgare cDNA clone
EBpi03_SQ001_M01 5', mRNA sequence
Length = 245
Score = 87.7 bits (185), Expect(2) = 7e-032
Identities = 37/40 (92%), Positives = 39/40 (97%)
Frame = +2 / +3
Query: 257 MTILGEDVHVCDEIYSNGGVVLPHKEIKSSILKPEIVM*S 376
MTILGEDVHV DE+YSNGGVVLPHKEIKSSILKPEIVM*+
Sbjct: 96 MTILGEDVHVGDEVYSNGGVVLPHKEIKSSILKPEIVM*A 215
Score = 75.8 bits (159), Expect(2) = 3e-023
Identities = 33/39 (84%), Positives = 35/39 (89%)
Frame = -2 / -2
Query: 372 HMTISGLRMLDLISLCGSTTPPLL*ISSQTWTSSPRMVM 256
HMTISGLRMLDLISLCG TTPPLL* SS T TSSPR+V+
Sbjct: 211 HMTISGLRMLDLISLCGRTTPPLL*TSSPTCTSSPRIVI 95
Score = 68.4 bits (143), Expect(2) = 7e-032
Identities = 23/32 (71%), Positives = 28/32 (87%)
Frame = +2 / +2
Query: 161 VMRAARVKQHACVSSSIIGWHSTVGKWARVEN 256
VMR R+K+HAC+ SIIGWHSTVG+WAR+EN
Sbjct: 2 VMRGVRIKKHACIGDSIIGWHSTVGQWARIEN 97
Score = 51.5 bits (106), Expect(2) = 3e-023
Identities = 21/32 (65%), Positives = 22/32 (68%)
Frame = -2 / -3
Query: 255 FSTRAHLPTVECQPMMLLETQACCLTRAARMT 160
FS RAH PTVECQPMML AC L R R+T
Sbjct: 96 FSMRAHCPTVECQPMMLSPMHACFLMRTPRIT 1
>gb|CV056920.1|CV056920 BNEL22a9 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL22a9 5' similar to GDP-D-mannose
pyrophosphorylase, mRNA sequence
Length = 555
Score = 136 bits (292), Expect(2) = 7e-032
Identities = 54/57 (94%), Positives = 57/57 (100%)
Frame = +2 / -2
Query: 203 SSIIGWHSTVGKWARVENMTILGEDVHVCDEIYSNGGVVLPHKEIKSSILKPEIVM* 373
+SI+GWHSTVGKWARVENMTILGEDVHVCDE+YSNGGVVLPHKEIKSSILKPEIVM*
Sbjct: 479 ASIVGWHSTVGKWARVENMTILGEDVHVCDEVYSNGGVVLPHKEIKSSILKPEIVM* 309
Score = 119 bits (254), Expect = 2e-026
Identities = 51/56 (91%), Positives = 51/56 (91%)
Frame = -2 / +1
Query: 372 HMTISGLRMLDLISLCGSTTPPLL*ISSQTWTSSPRMVMFSTRAHLPTVECQPMML 205
HMTISGLRMLDLISLCGSTTPPLL SSQT TSSPRMVMFSTRAHLPTVEC P ML
Sbjct: 310 HMTISGLRMLDLISLCGSTTPPLLYTSSQTCTSSPRMVMFSTRAHLPTVECHPTML 477
Score = 21.7 bits (41), Expect(2) = 7e-032
Identities = 8/15 (53%), Positives = 11/15 (73%)
Frame = +3 / -3
Query: 603 LSDEWK*QLLFV*KK 647
+SDEW+* + F KK
Sbjct: 58 VSDEWE*SISFTTKK 14
>gb|BU985075.1|BU985075 HF06A23r HF Hordeum vulgare subsp. vulgare cDNA clone HF06A23
5-PRIME, mRNA sequence
Length = 455
Score = 136 bits (291), Expect = 1e-031
Identities = 54/56 (96%), Positives = 56/56 (100%)
Frame = +2 / -1
Query: 206 SIIGWHSTVGKWARVENMTILGEDVHVCDEIYSNGGVVLPHKEIKSSILKPEIVM* 373
SI+GWHSTVGKWARVENMTILGEDVHVCDE+YSNGGVVLPHKEIKSSILKPEIVM*
Sbjct: 455 SIVGWHSTVGKWARVENMTILGEDVHVCDEVYSNGGVVLPHKEIKSSILKPEIVM* 288
Score = 117 bits (250), Expect = 6e-026
Identities = 50/55 (90%), Positives = 50/55 (90%)
Frame = -2 / +1
Query: 372 HMTISGLRMLDLISLCGSTTPPLL*ISSQTWTSSPRMVMFSTRAHLPTVECQPMM 208
HMTISGLRMLDLISLCGSTTPPLL SSQT TSSPRMVMFSTRAHLPTVEC P M
Sbjct: 289 HMTISGLRMLDLISLCGSTTPPLLYTSSQTCTSSPRMVMFSTRAHLPTVECHPTM 453
>gb|BQ760241.1|BQ760241 EBpi07_SQ003_K16_R pistil, 12 DPA, no treatment, cv Optic, EBpi07
Hordeum vulgare subsp. vulgare cDNA clone
EBpi07_SQ003_K16 5', mRNA sequence
Length = 357
Score = 77.6 bits (163), Expect(2) = 2e-027
Identities = 32/36 (88%), Positives = 35/36 (97%)
Frame = +2 / +1
Query: 269 GEDVHVCDEIYSNGGVVLPHKEIKSSILKPEIVM*S 376
G+DVHV DE+YSNGGVVLPHKEIKSSILKPEIVM*+
Sbjct: 76 GKDVHVGDEVYSNGGVVLPHKEIKSSILKPEIVM*A 183
Score = 63.8 bits (133), Expect(2) = 2e-027
Identities = 22/27 (81%), Positives = 27/27 (100%)
Frame = +2 / +2
Query: 194 CVSSSIIGWHSTVGKWARVENMTILGE 274
C+S+SIIGWHSTVG+WAR+ENMTILG+
Sbjct: 2 CISNSIIGWHSTVGQWARIENMTILGK 82
>gb|CV055258.1|CV055258 BNEL118f1 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL118f1 5' similar to COG1208,
GCD1, Nucleoside-diphosphate-sugar pyrophosphorylase
involved in lipopolysaccharide biosynthesis/translation
initiation factor 2B, gamma/epsilon subunits, mRNA
sequence
Length = 778
Score = 119 bits (254), Expect = 2e-026
Identities = 48/64 (75%), Positives = 53/64 (82%)
Frame = +2 / -1
Query: 182 KQHACVSSSIIGWHSTVGKWARVENMTILGEDVHVCDEIYSNGGVVLPHKEIKSSILKPE 361
+ HAC+S SI+GWH V KWARVENMTI GEDVHV DE+Y GV LPHKEI+SSILKPE
Sbjct: 511 RSHACISDSIVGWHFAVDKWARVENMTIQGEDVHVFDEVYIIAGVGLPHKEIESSILKPE 332
Query: 362 IVM* 373
IVM*
Sbjct: 331 IVM* 320
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 253,329,057
Number of Sequences: 312970
Number of extensions: 3930044
Number of successful extensions: 70264
Number of sequences better than 1.0e-020: 25
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6956340276
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)